Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043790 | XP_003589208.1 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 3.79e-57 | 181 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043790 | G7I3M4 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 1.81e-57 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0026470 | Msa1043790 | 0.815033 | 1.137181e-51 | -8.615850e-47 |
| Msa0026490 | Msa1043790 | 0.934710 | 2.646474e-96 | -8.615850e-47 |
| Msa0631200 | Msa1043790 | 0.821181 | 4.627919e-53 | -8.615850e-47 |
| Msa0669440 | Msa1043790 | 0.800991 | 1.111669e-48 | -8.615850e-47 |
| Msa0145280 | Msa1043790 | 0.820967 | 5.185674e-53 | -8.615850e-47 |
| Msa0145310 | Msa1043790 | 0.927347 | 1.333201e-91 | -8.615850e-47 |
| Msa0145410 | Msa1043790 | 0.947658 | 4.377110e-106 | -8.615850e-47 |
| Msa0165540 | Msa1043790 | 0.824177 | 9.295675e-54 | -8.615850e-47 |
| Msa0389810 | Msa1043790 | 0.804681 | 1.922428e-49 | -8.615850e-47 |
| Msa0466060 | Msa1043790 | 0.802829 | 4.658434e-49 | -8.615850e-47 |
| Msa0982270 | Msa1043790 | 0.800800 | 1.216202e-48 | -8.615850e-47 |
| Msa1043790 | Msa1183100 | -0.803282 | 3.755944e-49 | -8.615850e-47 |
| Msa1043790 | Msa1322190 | 0.821988 | 3.012782e-53 | -8.615850e-47 |
| Msa0309550 | Msa1043790 | 0.813492 | 2.490823e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1043790 | MtrunA17_Chr1g0153581 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 3.49e-61 | 181 |
| Msa1043790 | MtrunA17_Chr1g0153601 | 89.474 | 95 | 6 | 1 | 1 | 91 | 1 | 95 | 3.08e-55 | 166 |
| Msa1043790 | MtrunA17_Chr1g0153561 | 86.316 | 95 | 9 | 1 | 1 | 91 | 1 | 95 | 1.54e-54 | 164 |
| Msa1043790 | MtrunA17_Chr1g0153611 | 65.591 | 93 | 29 | 1 | 1 | 90 | 1 | 93 | 1.16e-38 | 124 |
| Msa1043790 | MtrunA17_Chr1g0153551 | 45.000 | 100 | 46 | 1 | 1 | 91 | 1 | 100 | 2.50e-19 | 75.5 |
| Msa1043790 | MtrunA17_Chr1g0153541 | 43.678 | 87 | 45 | 1 | 5 | 91 | 21 | 103 | 2.01e-16 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGATTAGTCCGTCTTTAT+TGG | 0.241517 | 7_2:-1955872 | None:intergenic |
| TATCTCCTTACCCGTGGTTT+TGG | 0.273881 | 7_2:+1955922 | Msa1043790:CDS |
| TGATCCATAGACACCCTCTT+TGG | 0.332546 | 7_2:-1955694 | None:intergenic |
| TATCCCAAAGAGGGTGTCTA+TGG | 0.383107 | 7_2:+1955690 | Msa1043790:CDS |
| GATCCATAGACACCCTCTTT+GGG | 0.423736 | 7_2:-1955693 | None:intergenic |
| CAACGATTGGCTCTCCATCC+AGG | 0.437823 | 7_2:-1955832 | None:intergenic |
| AGCTTTCATCAAACAACGAT+TGG | 0.498009 | 7_2:-1955845 | None:intergenic |
| TCTTGCCAAAACCACGGGTA+AGG | 0.519368 | 7_2:-1955927 | None:intergenic |
| CGATGGTCGTTACACAAAGA+AGG | 0.521782 | 7_2:+1955771 | Msa1043790:CDS |
| TAGGTTCTTACTACCCTGGA+TGG | 0.528156 | 7_2:+1955818 | Msa1043790:CDS |
| TTAGTTCTTGCCAAAACCAC+GGG | 0.528157 | 7_2:-1955932 | None:intergenic |
| TGGATCAAATCAACTTTGAA+AGG | 0.536831 | 7_2:+1955710 | Msa1043790:CDS |
| GATTTAGGTTCTTACTACCC+TGG | 0.543944 | 7_2:+1955814 | Msa1043790:CDS |
| AACGATTGGCTCTCCATCCA+GGG | 0.580860 | 7_2:-1955831 | None:intergenic |
| ATTAGTTCTTGCCAAAACCA+CGG | 0.585282 | 7_2:-1955933 | None:intergenic |
| GCCTGTGTTTATCCCAAAGA+GGG | 0.602626 | 7_2:+1955681 | Msa1043790:CDS |
| ATTGATTATCTCCTTACCCG+TGG | 0.619921 | 7_2:+1955916 | Msa1043790:CDS |
| AAAGCTGATGCCAATAAAGA+CGG | 0.628000 | 7_2:+1955862 | Msa1043790:CDS |
| TGCCTGTGTTTATCCCAAAG+AGG | 0.630941 | 7_2:+1955680 | Msa1043790:CDS |
| ACCCTCTTTGGGATAAACAC+AGG | 0.645492 | 7_2:-1955682 | None:intergenic |
| AGACGGACTAATCAGTGGCG+AGG | 0.659853 | 7_2:+1955879 | Msa1043790:CDS |
| AATAAAGACGGACTAATCAG+TGG | 0.668016 | 7_2:+1955874 | Msa1043790:CDS |
| AAGGCGGATATCAATAACGA+TGG | 0.697636 | 7_2:+1955754 | Msa1043790:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGAACATTATAGAAAAAT+TGG | + | chr7_2:1955729-1955748 | Msa1043790:CDS | 20.0% |
| ! | CATTATAGAAAAATTGGAGA+AGG | + | chr7_2:1955735-1955754 | Msa1043790:CDS | 25.0% |
| !!! | AAAAAAGCTCTAAAGGATTT+AGG | + | chr7_2:1955799-1955818 | Msa1043790:CDS | 25.0% |
| TGGATCAAATCAACTTTGAA+AGG | + | chr7_2:1955710-1955729 | Msa1043790:CDS | 30.0% | |
| TATAGAAAAATTGGAGAAGG+CGG | + | chr7_2:1955738-1955757 | Msa1043790:CDS | 30.0% | |
| ! | GGAACTCAAAAAAGCTCTAA+AGG | + | chr7_2:1955792-1955811 | Msa1043790:CDS | 35.0% |
| AGCTTTCATCAAACAACGAT+TGG | - | chr7_2:1955848-1955867 | None:intergenic | 35.0% | |
| AAAGCTGATGCCAATAAAGA+CGG | + | chr7_2:1955862-1955881 | Msa1043790:CDS | 35.0% | |
| ! | ACTGATTAGTCCGTCTTTAT+TGG | - | chr7_2:1955875-1955894 | None:intergenic | 35.0% |
| !! | AATAAAGACGGACTAATCAG+TGG | + | chr7_2:1955874-1955893 | Msa1043790:CDS | 35.0% |
| AAGGCGGATATCAATAACGA+TGG | + | chr7_2:1955754-1955773 | Msa1043790:CDS | 40.0% | |
| ! | GATTTAGGTTCTTACTACCC+TGG | + | chr7_2:1955814-1955833 | Msa1043790:CDS | 40.0% |
| ATTGATTATCTCCTTACCCG+TGG | + | chr7_2:1955916-1955935 | Msa1043790:CDS | 40.0% | |
| ! | TTAGTTCTTGCCAAAACCAC+GGG | - | chr7_2:1955935-1955954 | None:intergenic | 40.0% |
| TGCCTGTGTTTATCCCAAAG+AGG | + | chr7_2:1955680-1955699 | Msa1043790:CDS | 45.0% | |
| GCCTGTGTTTATCCCAAAGA+GGG | + | chr7_2:1955681-1955700 | Msa1043790:CDS | 45.0% | |
| !! | ACCCTCTTTGGGATAAACAC+AGG | - | chr7_2:1955685-1955704 | None:intergenic | 45.0% |
| !! | TATCCCAAAGAGGGTGTCTA+TGG | + | chr7_2:1955690-1955709 | Msa1043790:CDS | 45.0% |
| GATCCATAGACACCCTCTTT+GGG | - | chr7_2:1955696-1955715 | None:intergenic | 45.0% | |
| TGATCCATAGACACCCTCTT+TGG | - | chr7_2:1955697-1955716 | None:intergenic | 45.0% | |
| CGATGGTCGTTACACAAAGA+AGG | + | chr7_2:1955771-1955790 | Msa1043790:CDS | 45.0% | |
| TAGGTTCTTACTACCCTGGA+TGG | + | chr7_2:1955818-1955837 | Msa1043790:CDS | 45.0% | |
| ! | TATCTCCTTACCCGTGGTTT+TGG | + | chr7_2:1955922-1955941 | Msa1043790:CDS | 45.0% |
| ! | AACGATTGGCTCTCCATCCA+GGG | - | chr7_2:1955834-1955853 | None:intergenic | 50.0% |
| TCTTGCCAAAACCACGGGTA+AGG | - | chr7_2:1955930-1955949 | None:intergenic | 50.0% | |
| ! | CAACGATTGGCTCTCCATCC+AGG | - | chr7_2:1955835-1955854 | None:intergenic | 55.0% |
| !! | AGACGGACTAATCAGTGGCG+AGG | + | chr7_2:1955879-1955898 | Msa1043790:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 1955679 | 1955954 | 1955679 | ID=Msa1043790;Name=Msa1043790 |
| chr7_2 | mRNA | 1955679 | 1955954 | 1955679 | ID=Msa1043790-mRNA-1;Parent=Msa1043790;Name=Msa1043790-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|91 |
| chr7_2 | exon | 1955679 | 1955954 | 1955679 | ID=Msa1043790-mRNA-1:exon:401;Parent=Msa1043790-mRNA-1 |
| chr7_2 | CDS | 1955679 | 1955954 | 1955679 | ID=Msa1043790-mRNA-1:cds;Parent=Msa1043790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
