AlfalfaGEDB | Msa0145280

M. sativa cultivar zhongmu-4 / Msa0145280

KEGG: -
GO: -
PFAM: PF13202.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145280	XP_003589210.1	91.304	69	6	0	62	130	27	95	4.56e-37	132

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145280	G7I3M6	91.304	69	6	0	62	130	27	95	2.18e-37	132

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0026470	Msa0145280	0.854102	1.539475e-61	-8.615850e-47
Msa0026490	Msa0145280	0.816729	4.760035e-52	-8.615850e-47
Msa0145280	Msa0145410	0.810182	1.308802e-50	-8.615850e-47
Msa0145280	Msa1043790	0.820967	5.185674e-53	-8.615850e-47
Msa0145280	Msa1043810	0.872863	2.292848e-67	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145280	MtrunA17_Chr1g0153601	91.304	69	6	62	130	27	95	4.20e-41	132
Msa0145280	MtrunA17_Chr1g0153581	86.957	69	9	62	130	27	95	8.44e-38	123
Msa0145280	MtrunA17_Chr1g0153561	75.325	77	19	54	130	19	95	4.28e-37	122
Msa0145280	MtrunA17_Chr1g0153611	75.385	65	16	65	129	29	93	3.07e-30	104
Msa0145280	MtrunA17_Chr1g0153551	53.030	66	31	65	130	35	100	2.26e-16	69.3
Msa0145280	MtrunA17_Chr1g0153541	51.515	66	32	65	130	38	103	4.21e-15	66.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 32 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATATGGCTCTCCATCCA+GGG	0.594858	1_4:+4954110	None:intergenic
AAAGCTGATGCCAACAAAGA+CGG	0.620958	1_4:-4954079	Msa0145280:CDS
AAATGGATAGTAAGGATGTT+GGG	0.498325	1_4:-4955046	Msa0145280:CDS
AACAAAGACGGACAAATCAG+TGG	0.638335	1_4:-4954067	Msa0145280:CDS
AAGGCAGATATCAATAAAGA+CGG	0.642966	1_4:-4954187	Msa0145280:CDS
AATGCCTGTGTTTATCCCTT+CGG	0.462697	1_4:-4954274	Msa0145280:CDS
ACCGGCTTTGGTTTGTCCGA+AGG	0.472684	1_4:+4954258	None:intergenic
ACTGATTTGTCCGTCTTTGT+TGG	0.364609	1_4:+4954069	None:intergenic
AGACGGACAAATCAGTGGCG+AGG	0.670203	1_4:-4954062	Msa0145280:CDS
AGACGGTCGTTACACAAAGG+AGG	0.680084	1_4:-4954170	Msa0145280:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGATTGAAAATGTGATTGT+TGG	-	chr1_4:4954167-4954186	Msa0145280:CDS	30.0%
TATATTTAACCAAGACTCAG+AGG	+	chr1_4:4954227-4954246	None:intergenic	30.0%
GTTCAAAAGTTGTCAAAAAG+TGG	+	chr1_4:4954271-4954290	None:intergenic	30.0%
AAAACAATGGAGAGAGAAAA+AGG	+	chr1_4:4954338-4954357	None:intergenic	30.0%
TCAACAAAGTTGTCATCAAA+GGG	-	chr1_4:4954390-4954409	Msa0145280:intron	30.0%
TGATTATCATATTGAGGAGA+GGG	+	chr1_4:4954710-4954729	None:intergenic	30.0%
ATGATTATCATATTGAGGAG+AGG	+	chr1_4:4954711-4954730	None:intergenic	30.0%
AAGGCAGATATCAATAAAGA+CGG	-	chr1_4:4954866-4954885	Msa0145280:intron	30.0%
TGGATTGAAAATGTGATTGT+TGG	-	chr1_4:4954167-4954186	Msa0145280:CDS	30.0%
TATATTTAACCAAGACTCAG+AGG	+	chr1_4:4954227-4954246	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	4954009	4955066	4954009	ID=Msa0145280;Name=Msa0145280
chr1_4	mRNA	4954009	4955066	4954009	ID=Msa0145280-mRNA-1;Parent=Msa0145280;Name=Msa0145280-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|130
chr1_4	exon	4954989	4955066	4954989	ID=Msa0145280-mRNA-1:exon:2068;Parent=Msa0145280-mRNA-1
chr1_4	exon	4954009	4954323	4954009	ID=Msa0145280-mRNA-1:exon:2067;Parent=Msa0145280-mRNA-1
chr1_4	CDS	4954989	4955066	4954989	ID=Msa0145280-mRNA-1:cds;Parent=Msa0145280-mRNA-1
chr1_4	CDS	4954009	4954323	4954009	ID=Msa0145280-mRNA-1:cds;Parent=Msa0145280-mRNA-1

Gene Sequence

>Msa0145280

ATGGATAGTAAGGATGTTGGGAAACTTGCTTTAGATTACTTAGATTACTTTGGTGTTATTAGTAGGAGTGCTCCAAAAGAGATCAATCAACTCTTAACATTTCAAAATGCCTGTGTTTATCCCTTCGGACAAACCAAAGCCGGTGCCTATGGACCAAATCAACTTCGAAAGGAACATTATCGAAAAATTGGAAAGGCAGATATCAATAAAGACGGTCGTTACACAAAGGAGGAACTCAAAAAAGCTCTAAAGGATTTAGGTTCTTACTACCCTGGATGGAGAGCCATATTTTGTTTCATGAAAGCTGATGCCAACAAAGACGGACAAATCAGTGGCGAGGAAATCGACACTTTAGTTGATTATCTCCTTAGCCGTGGTTTTGGCAAGAACTAA

Protein sequence

>Msa0145280

MDSKDVGKLALDYLDYFGVISRSAPKEINQLLTFQNACVYPFGQTKAGAYGPNQLRKEHYRKIGKADINKDGRYTKEELKKALKDLGSYYPGWRAIFCFMKADANKDGQISGEEIDTLVDYLLSRGFGKN*