AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0145280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0145280 MtrunA17_Chr1g0153601 91.304 69 6 0 62 130 27 95 4.20e-41 132
Msa0145280 MtrunA17_Chr1g0153581 86.957 69 9 0 62 130 27 95 8.44e-38 123
Msa0145280 MtrunA17_Chr1g0153561 75.325 77 19 0 54 130 19 95 4.28e-37 122
Msa0145280 MtrunA17_Chr1g0153611 75.385 65 16 0 65 129 29 93 3.07e-30 104
Msa0145280 MtrunA17_Chr1g0153551 53.030 66 31 0 65 130 35 100 2.26e-16 69.3
Msa0145280 MtrunA17_Chr1g0153541 51.515 66 32 0 65 130 38 103 4.21e-15 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCCTTAGCCGTGGTTT+TGG 0.295002 1_4:-4954019 Msa0145280:CDS
TGTTCCTTTCGAAGTTGATT+TGG 0.344741 1_4:+4954226 None:intergenic
ATTACTTTGGTGTTATTAGT+AGG 0.353215 1_4:-4955001 Msa0145280:CDS
AGCTTTCATGAAACAAAATA+TGG 0.357016 1_4:+4954096 None:intergenic
ACTGATTTGTCCGTCTTTGT+TGG 0.364609 1_4:+4954069 None:intergenic
CAAAATATGGCTCTCCATCC+AGG 0.411421 1_4:+4954109 None:intergenic
TTAGATTACTTAGATTACTT+TGG 0.425054 1_4:-4955014 Msa0145280:CDS
TGGTCCATAGGCACCGGCTT+TGG 0.436522 1_4:+4954246 None:intergenic
AGTATTGGAAATGGATAGTA+AGG 0.441337 1_4:-4955054 None:intergenic
CAAACCAAAGCCGGTGCCTA+TGG 0.455404 1_4:-4954250 Msa0145280:CDS
AATGCCTGTGTTTATCCCTT+CGG 0.462697 1_4:-4954274 Msa0145280:CDS
ACCGGCTTTGGTTTGTCCGA+AGG 0.472684 1_4:+4954258 None:intergenic
GAAATGGATAGTAAGGATGT+TGG 0.477479 1_4:-4955047 Msa0145280:CDS
CCGGCTTTGGTTTGTCCGAA+GGG 0.495436 1_4:+4954259 None:intergenic
AAATGGATAGTAAGGATGTT+GGG 0.498325 1_4:-4955046 Msa0145280:CDS
TAGGTTCTTACTACCCTGGA+TGG 0.528156 1_4:-4954123 Msa0145280:CDS
TCGAAGTTGATTTGGTCCAT+AGG 0.530320 1_4:+4954234 None:intergenic
GATTTAGGTTCTTACTACCC+TGG 0.543944 1_4:-4954127 Msa0145280:CDS
TCTTGCCAAAACCACGGCTA+AGG 0.544398 1_4:+4954014 None:intergenic
CCCTTCGGACAAACCAAAGC+CGG 0.550311 1_4:-4954259 Msa0145280:CDS
TTGATTTGGTCCATAGGCAC+CGG 0.550954 1_4:+4954240 None:intergenic
AAAATATGGCTCTCCATCCA+GGG 0.594858 1_4:+4954110 None:intergenic
TGGACCAAATCAACTTCGAA+AGG 0.599482 1_4:-4954230 Msa0145280:CDS
GTTGATTATCTCCTTAGCCG+TGG 0.610542 1_4:-4954025 Msa0145280:CDS
AAAGCTGATGCCAACAAAGA+CGG 0.620958 1_4:-4954079 Msa0145280:CDS
ATTAGTTCTTGCCAAAACCA+CGG 0.621014 1_4:+4954008 None:intergenic
AACAAAGACGGACAAATCAG+TGG 0.638335 1_4:-4954067 Msa0145280:CDS
AAGGCAGATATCAATAAAGA+CGG 0.642966 1_4:-4954187 Msa0145280:CDS
TTGTCCGAAGGGATAAACAC+AGG 0.660183 1_4:+4954270 None:intergenic
AGACGGACAAATCAGTGGCG+AGG 0.670203 1_4:-4954062 Msa0145280:CDS
TAAAGACGGTCGTTACACAA+AGG 0.678917 1_4:-4954173 Msa0145280:CDS
AGACGGTCGTTACACAAAGG+AGG 0.680084 1_4:-4954170 Msa0145280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGCTTTTATATTTTAAGAT+AGG + chr1_4:4954586-4954605 None:intergenic 15.0%
!!! TGAATGTTTTTGAAATAAAA+TGG + chr1_4:4954655-4954674 None:intergenic 15.0%
!!! TTGCTTTTATATTTTAAGAT+AGG + chr1_4:4954586-4954605 None:intergenic 15.0%
!!! TGAATGTTTTTGAAATAAAA+TGG + chr1_4:4954655-4954674 None:intergenic 15.0%
!! TTAGATTACTTAGATTACTT+TGG - chr1_4:4954039-4954058 Msa0145280:CDS 20.0%
!!! AAAGGATAAACAATTACTTT+TGG + chr1_4:4954084-4954103 None:intergenic 20.0%
!! ATACATTCATAGAAAAGAAA+AGG + chr1_4:4954102-4954121 None:intergenic 20.0%
!! TCACATATATATACTGAAAA+TGG - chr1_4:4954121-4954140 Msa0145280:CDS 20.0%
!! TATATATACTGAAAATGGAT+TGG - chr1_4:4954126-4954145 Msa0145280:CDS 20.0%
!!! ACAAGTTTTTTTCTACAAAA+AGG - chr1_4:4954442-4954461 Msa0145280:intron 20.0%
!!! TGAGATATTAGCTTTTAAAT+TGG - chr1_4:4954469-4954488 Msa0145280:intron 20.0%
!! TTAGATTACTTAGATTACTT+TGG - chr1_4:4954039-4954058 Msa0145280:CDS 20.0%
!!! AAAGGATAAACAATTACTTT+TGG + chr1_4:4954084-4954103 None:intergenic 20.0%
!! ATACATTCATAGAAAAGAAA+AGG + chr1_4:4954102-4954121 None:intergenic 20.0%
!! TCACATATATATACTGAAAA+TGG - chr1_4:4954121-4954140 Msa0145280:CDS 20.0%
!! TATATATACTGAAAATGGAT+TGG - chr1_4:4954126-4954145 Msa0145280:CDS 20.0%
!!! ACAAGTTTTTTTCTACAAAA+AGG - chr1_4:4954442-4954461 Msa0145280:intron 20.0%
!!! TGAGATATTAGCTTTTAAAT+TGG - chr1_4:4954469-4954488 Msa0145280:intron 20.0%
!!! ATTACTTTGGTGTTATTAGT+AGG - chr1_4:4954052-4954071 Msa0145280:CDS 25.0%
! TCTTATTGGTCAAAAAACAA+TGG + chr1_4:4954351-4954370 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr1_4:4954365-4954384 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr1_4:4954364-4954383 Msa0145280:intron 25.0%
! TGTTCAAGATATTTGACTAA+AGG - chr1_4:4954544-4954563 Msa0145280:intron 25.0%
! GTTCAAGATATTTGACTAAA+GGG - chr1_4:4954545-4954564 Msa0145280:intron 25.0%
! TGTCTATGATTATCATATTG+AGG + chr1_4:4954716-4954735 None:intergenic 25.0%
! TAGACAACTTGATAGAAATT+AGG - chr1_4:4954730-4954749 Msa0145280:intron 25.0%
! AAGGAACATTATCGAAAAAT+TGG - chr1_4:4954842-4954861 Msa0145280:intron 25.0%
! ACATTATCGAAAAATTGGAA+AGG - chr1_4:4954847-4954866 Msa0145280:intron 25.0%
!!! AAAAAAGCTCTAAAGGATTT+AGG - chr1_4:4954911-4954930 Msa0145280:intron 25.0%
! AGCTTTCATGAAACAAAATA+TGG + chr1_4:4954960-4954979 None:intergenic 25.0%
!!! ATTACTTTGGTGTTATTAGT+AGG - chr1_4:4954052-4954071 Msa0145280:CDS 25.0%
! TCTTATTGGTCAAAAAACAA+TGG + chr1_4:4954351-4954370 None:intergenic 25.0%
!!! TTGTTTTTCTCACTTCTTAT+TGG + chr1_4:4954365-4954384 None:intergenic 25.0%
! AATAAGAAGTGAGAAAAACA+AGG - chr1_4:4954364-4954383 Msa0145280:intron 25.0%
! TGTTCAAGATATTTGACTAA+AGG - chr1_4:4954544-4954563 Msa0145280:intron 25.0%
! GTTCAAGATATTTGACTAAA+GGG - chr1_4:4954545-4954564 Msa0145280:intron 25.0%
! TGTCTATGATTATCATATTG+AGG + chr1_4:4954716-4954735 None:intergenic 25.0%
! TAGACAACTTGATAGAAATT+AGG - chr1_4:4954730-4954749 Msa0145280:intron 25.0%
! AAGGAACATTATCGAAAAAT+TGG - chr1_4:4954842-4954861 Msa0145280:intron 25.0%
! ACATTATCGAAAAATTGGAA+AGG - chr1_4:4954847-4954866 Msa0145280:intron 25.0%
!!! AAAAAAGCTCTAAAGGATTT+AGG - chr1_4:4954911-4954930 Msa0145280:intron 25.0%
! AGCTTTCATGAAACAAAATA+TGG + chr1_4:4954960-4954979 None:intergenic 25.0%
TGGATTGAAAATGTGATTGT+TGG - chr1_4:4954167-4954186 Msa0145280:CDS 30.0%
TATATTTAACCAAGACTCAG+AGG + chr1_4:4954227-4954246 None:intergenic 30.0%
GTTCAAAAGTTGTCAAAAAG+TGG + chr1_4:4954271-4954290 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr1_4:4954338-4954357 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+GGG - chr1_4:4954390-4954409 Msa0145280:intron 30.0%
!!! GTTTATTTTTTGAGTGTGCT+TGG + chr1_4:4954519-4954538 None:intergenic 30.0%
! TTTAAGATAGGATTCCAAAC+TGG + chr1_4:4954574-4954593 None:intergenic 30.0%
TGATTATCATATTGAGGAGA+GGG + chr1_4:4954710-4954729 None:intergenic 30.0%
ATGATTATCATATTGAGGAG+AGG + chr1_4:4954711-4954730 None:intergenic 30.0%
AAGGCAGATATCAATAAAGA+CGG - chr1_4:4954866-4954885 Msa0145280:intron 30.0%
TGGATTGAAAATGTGATTGT+TGG - chr1_4:4954167-4954186 Msa0145280:CDS 30.0%
TATATTTAACCAAGACTCAG+AGG + chr1_4:4954227-4954246 None:intergenic 30.0%
GTTCAAAAGTTGTCAAAAAG+TGG + chr1_4:4954271-4954290 None:intergenic 30.0%
AAAACAATGGAGAGAGAAAA+AGG + chr1_4:4954338-4954357 None:intergenic 30.0%
TCAACAAAGTTGTCATCAAA+GGG - chr1_4:4954390-4954409 Msa0145280:intron 30.0%
!!! GTTTATTTTTTGAGTGTGCT+TGG + chr1_4:4954519-4954538 None:intergenic 30.0%
! TTTAAGATAGGATTCCAAAC+TGG + chr1_4:4954574-4954593 None:intergenic 30.0%
TGATTATCATATTGAGGAGA+GGG + chr1_4:4954710-4954729 None:intergenic 30.0%
ATGATTATCATATTGAGGAG+AGG + chr1_4:4954711-4954730 None:intergenic 30.0%
AAGGCAGATATCAATAAAGA+CGG - chr1_4:4954866-4954885 Msa0145280:intron 30.0%
GGAAACAATTCATGCTTGAT+TGG - chr1_4:4954147-4954166 Msa0145280:CDS 35.0%
ATGTGATTGTTGGCAAAACT+AGG - chr1_4:4954177-4954196 Msa0145280:CDS 35.0%
GTCAACAAAGTTGTCATCAA+AGG - chr1_4:4954389-4954408 Msa0145280:intron 35.0%
GATTATCATATTGAGGAGAG+GGG + chr1_4:4954709-4954728 None:intergenic 35.0%
! TGTTCCTTTCGAAGTTGATT+TGG + chr1_4:4954830-4954849 None:intergenic 35.0%
! GGAACTCAAAAAAGCTCTAA+AGG - chr1_4:4954904-4954923 Msa0145280:intron 35.0%
GGAAACAATTCATGCTTGAT+TGG - chr1_4:4954147-4954166 Msa0145280:CDS 35.0%
ATGTGATTGTTGGCAAAACT+AGG - chr1_4:4954177-4954196 Msa0145280:CDS 35.0%
GTCAACAAAGTTGTCATCAA+AGG - chr1_4:4954389-4954408 Msa0145280:intron 35.0%
GATTATCATATTGAGGAGAG+GGG + chr1_4:4954709-4954728 None:intergenic 35.0%
! TGTTCCTTTCGAAGTTGATT+TGG + chr1_4:4954830-4954849 None:intergenic 35.0%
! GGAACTCAAAAAAGCTCTAA+AGG - chr1_4:4954904-4954923 Msa0145280:intron 35.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr1_4:4954328-4954347 None:intergenic 40.0%
ATTGAGGAGAGGGGTTAAAT+AGG + chr1_4:4954700-4954719 None:intergenic 40.0%
AATGCCTGTGTTTATCCCTT+CGG - chr1_4:4954779-4954798 Msa0145280:intron 40.0%
!! TCGAAGTTGATTTGGTCCAT+AGG + chr1_4:4954822-4954841 None:intergenic 40.0%
TGGACCAAATCAACTTCGAA+AGG - chr1_4:4954823-4954842 Msa0145280:intron 40.0%
TAAAGACGGTCGTTACACAA+AGG - chr1_4:4954880-4954899 Msa0145280:intron 40.0%
! GATTTAGGTTCTTACTACCC+TGG - chr1_4:4954926-4954945 Msa0145280:intron 40.0%
AAAATATGGCTCTCCATCCA+GGG + chr1_4:4954946-4954965 None:intergenic 40.0%
AAAGCTGATGCCAACAAAGA+CGG - chr1_4:4954974-4954993 Msa0145280:intron 40.0%
!! ACTGATTTGTCCGTCTTTGT+TGG + chr1_4:4954987-4955006 None:intergenic 40.0%
AACAAAGACGGACAAATCAG+TGG - chr1_4:4954986-4955005 Msa0145280:intron 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG + chr1_4:4954328-4954347 None:intergenic 40.0%
ATTGAGGAGAGGGGTTAAAT+AGG + chr1_4:4954700-4954719 None:intergenic 40.0%
AATGCCTGTGTTTATCCCTT+CGG - chr1_4:4954779-4954798 Msa0145280:intron 40.0%
!! TCGAAGTTGATTTGGTCCAT+AGG + chr1_4:4954822-4954841 None:intergenic 40.0%
TGGACCAAATCAACTTCGAA+AGG - chr1_4:4954823-4954842 Msa0145280:intron 40.0%
TAAAGACGGTCGTTACACAA+AGG - chr1_4:4954880-4954899 Msa0145280:intron 40.0%
! GATTTAGGTTCTTACTACCC+TGG - chr1_4:4954926-4954945 Msa0145280:intron 40.0%
AAAATATGGCTCTCCATCCA+GGG + chr1_4:4954946-4954965 None:intergenic 40.0%
AAAGCTGATGCCAACAAAGA+CGG - chr1_4:4954974-4954993 Msa0145280:intron 40.0%
!! ACTGATTTGTCCGTCTTTGT+TGG + chr1_4:4954987-4955006 None:intergenic 40.0%
AACAAAGACGGACAAATCAG+TGG - chr1_4:4954986-4955005 Msa0145280:intron 40.0%
TAGCTAAGTCCTCTGAGTCT+TGG - chr1_4:4954215-4954234 Msa0145280:CDS 45.0%
TGACTAAAGGGTGACCAGTT+TGG - chr1_4:4954557-4954576 Msa0145280:intron 45.0%
TTGTCCGAAGGGATAAACAC+AGG + chr1_4:4954786-4954805 None:intergenic 45.0%
!! TTGATTTGGTCCATAGGCAC+CGG + chr1_4:4954816-4954835 None:intergenic 45.0%
TAGGTTCTTACTACCCTGGA+TGG - chr1_4:4954930-4954949 Msa0145280:intron 45.0%
CAAAATATGGCTCTCCATCC+AGG + chr1_4:4954947-4954966 None:intergenic 45.0%
GTTGATTATCTCCTTAGCCG+TGG - chr1_4:4955028-4955047 Msa0145280:CDS 45.0%
! TATCTCCTTAGCCGTGGTTT+TGG - chr1_4:4955034-4955053 Msa0145280:CDS 45.0%
TAGCTAAGTCCTCTGAGTCT+TGG - chr1_4:4954215-4954234 Msa0145280:CDS 45.0%
TGACTAAAGGGTGACCAGTT+TGG - chr1_4:4954557-4954576 Msa0145280:intron 45.0%
TTGTCCGAAGGGATAAACAC+AGG + chr1_4:4954786-4954805 None:intergenic 45.0%
!! TTGATTTGGTCCATAGGCAC+CGG + chr1_4:4954816-4954835 None:intergenic 45.0%
TAGGTTCTTACTACCCTGGA+TGG - chr1_4:4954930-4954949 Msa0145280:intron 45.0%
CAAAATATGGCTCTCCATCC+AGG + chr1_4:4954947-4954966 None:intergenic 45.0%
GTTGATTATCTCCTTAGCCG+TGG - chr1_4:4955028-4955047 Msa0145280:CDS 45.0%
! TATCTCCTTAGCCGTGGTTT+TGG - chr1_4:4955034-4955053 Msa0145280:CDS 45.0%
AGACGGTCGTTACACAAAGG+AGG - chr1_4:4954883-4954902 Msa0145280:intron 50.0%
TCTTGCCAAAACCACGGCTA+AGG + chr1_4:4955042-4955061 None:intergenic 50.0%
AGACGGTCGTTACACAAAGG+AGG - chr1_4:4954883-4954902 Msa0145280:intron 50.0%
TCTTGCCAAAACCACGGCTA+AGG + chr1_4:4955042-4955061 None:intergenic 50.0%
!! CCGGCTTTGGTTTGTCCGAA+GGG + chr1_4:4954797-4954816 None:intergenic 55.0%
CCCTTCGGACAAACCAAAGC+CGG - chr1_4:4954794-4954813 Msa0145280:intron 55.0%
!! ACCGGCTTTGGTTTGTCCGA+AGG + chr1_4:4954798-4954817 None:intergenic 55.0%
CAAACCAAAGCCGGTGCCTA+TGG - chr1_4:4954803-4954822 Msa0145280:intron 55.0%
AGACGGACAAATCAGTGGCG+AGG - chr1_4:4954991-4955010 Msa0145280:CDS 55.0%
!! CCGGCTTTGGTTTGTCCGAA+GGG + chr1_4:4954797-4954816 None:intergenic 55.0%
CCCTTCGGACAAACCAAAGC+CGG - chr1_4:4954794-4954813 Msa0145280:intron 55.0%
!! ACCGGCTTTGGTTTGTCCGA+AGG + chr1_4:4954798-4954817 None:intergenic 55.0%
CAAACCAAAGCCGGTGCCTA+TGG - chr1_4:4954803-4954822 Msa0145280:intron 55.0%
AGACGGACAAATCAGTGGCG+AGG - chr1_4:4954991-4955010 Msa0145280:CDS 55.0%
!! TGGTCCATAGGCACCGGCTT+TGG + chr1_4:4954810-4954829 None:intergenic 60.0%
!! TGGTCCATAGGCACCGGCTT+TGG + chr1_4:4954810-4954829 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 4954009 4955066 4954009 ID=Msa0145280;Name=Msa0145280
chr1_4 mRNA 4954009 4955066 4954009 ID=Msa0145280-mRNA-1;Parent=Msa0145280;Name=Msa0145280-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|0.5|1|1|2|0|130
chr1_4 exon 4954989 4955066 4954989 ID=Msa0145280-mRNA-1:exon:2068;Parent=Msa0145280-mRNA-1
chr1_4 exon 4954009 4954323 4954009 ID=Msa0145280-mRNA-1:exon:2067;Parent=Msa0145280-mRNA-1
chr1_4 CDS 4954989 4955066 4954989 ID=Msa0145280-mRNA-1:cds;Parent=Msa0145280-mRNA-1
chr1_4 CDS 4954009 4954323 4954009 ID=Msa0145280-mRNA-1:cds;Parent=Msa0145280-mRNA-1
Gene Sequence

>Msa0145280

ATGGATAGTAAGGATGTTGGGAAACTTGCTTTAGATTACTTAGATTACTTTGGTGTTATTAGTAGGAGTGCTCCAAAAGAGATCAATCAACTCTTAACATTTCAAAATGCCTGTGTTTATCCCTTCGGACAAACCAAAGCCGGTGCCTATGGACCAAATCAACTTCGAAAGGAACATTATCGAAAAATTGGAAAGGCAGATATCAATAAAGACGGTCGTTACACAAAGGAGGAACTCAAAAAAGCTCTAAAGGATTTAGGTTCTTACTACCCTGGATGGAGAGCCATATTTTGTTTCATGAAAGCTGATGCCAACAAAGACGGACAAATCAGTGGCGAGGAAATCGACACTTTAGTTGATTATCTCCTTAGCCGTGGTTTTGGCAAGAACTAA

Protein sequence

>Msa0145280

MDSKDVGKLALDYLDYFGVISRSAPKEINQLLTFQNACVYPFGQTKAGAYGPNQLRKEHYRKIGKADINKDGRYTKEELKKALKDLGSYYPGWRAIFCFMKADANKDGQISGEEIDTLVDYLLSRGFGKN*