Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0012060 | Msa0026490 | 0.801061 | 1.075566e-48 | -8.615850e-47 |
| Msa0017510 | Msa0026490 | 0.802502 | 5.443535e-49 | -8.615850e-47 |
| Msa0026470 | Msa0026490 | 0.807288 | 5.439177e-50 | -8.615850e-47 |
| Msa0026490 | Msa0037700 | 0.819299 | 1.249528e-52 | -8.615850e-47 |
| Msa0026490 | Msa0057520 | 0.814889 | 1.223973e-51 | -8.615850e-47 |
| Msa0026490 | Msa0063350 | -0.810904 | 9.141972e-51 | -8.615850e-47 |
| Msa0026490 | Msa0091210 | -0.800202 | 1.609441e-48 | -8.615850e-47 |
| Msa0026490 | Msa0112750 | 0.800269 | 1.559766e-48 | -8.615850e-47 |
| Msa0026490 | Msa0133130 | 0.807211 | 5.646288e-50 | -8.615850e-47 |
| Msa0026490 | Msa0145280 | 0.816729 | 4.760035e-52 | -8.615850e-47 |
| Msa0026490 | Msa0145310 | 0.967127 | 7.503493e-127 | -8.615850e-47 |
| Msa0026490 | Msa0145410 | 0.984195 | 7.380761e-160 | -8.615850e-47 |
| Msa0026490 | Msa0157290 | 0.802980 | 4.336470e-49 | -8.615850e-47 |
| Msa0026490 | Msa0165540 | 0.806808 | 6.870732e-50 | -8.615850e-47 |
| Msa0026490 | Msa0173990 | 0.803916 | 2.773722e-49 | -8.615850e-47 |
| Msa0026490 | Msa0178860 | -0.809001 | 2.348118e-50 | -8.615850e-47 |
| Msa0026490 | Msa0223740 | 0.827878 | 1.226456e-54 | -8.615850e-47 |
| Msa0026490 | Msa0302580 | -0.819288 | 1.256588e-52 | -8.615850e-47 |
| Msa0026490 | Msa0306210 | -0.806096 | 9.712319e-50 | -8.615850e-47 |
| Msa0026490 | Msa0309550 | 0.822758 | 1.995292e-53 | -8.615850e-47 |
| Msa0026490 | Msa0340450 | 0.800610 | 1.329461e-48 | -8.615850e-47 |
| Msa0026490 | Msa0379640 | 0.804122 | 2.513112e-49 | -8.615850e-47 |
| Msa0026490 | Msa0389810 | 0.815328 | 9.781898e-52 | -8.615850e-47 |
| Msa0026490 | Msa0431060 | 0.805465 | 1.317683e-49 | -8.615850e-47 |
| Msa0026490 | Msa0466390 | 0.809391 | 1.937032e-50 | -8.615850e-47 |
| Msa0026490 | Msa0468130 | 0.803481 | 3.415754e-49 | -8.615850e-47 |
| Msa0026490 | Msa0477130 | -0.800424 | 1.450365e-48 | -8.615850e-47 |
| Msa0026490 | Msa0484780 | 0.812303 | 4.536682e-51 | -8.615850e-47 |
| Msa0026490 | Msa0512900 | 0.803162 | 3.975859e-49 | -8.615850e-47 |
| Msa0026490 | Msa0534730 | 0.812866 | 3.417802e-51 | -8.615850e-47 |
| Msa0026490 | Msa0536220 | 0.806570 | 7.714432e-50 | -8.615850e-47 |
| Msa0026490 | Msa0575470 | 0.812249 | 4.661539e-51 | -8.615850e-47 |
| Msa0026490 | Msa0599890 | 0.803112 | 4.072819e-49 | -8.615850e-47 |
| Msa0026490 | Msa0631200 | 0.852227 | 5.300834e-61 | -8.615850e-47 |
| Msa0026490 | Msa0669440 | 0.815801 | 7.675163e-52 | -8.615850e-47 |
| Msa0026490 | Msa0708860 | 0.805047 | 1.611698e-49 | -8.615850e-47 |
| Msa0026490 | Msa0846810 | 0.800157 | 1.644451e-48 | -8.615850e-47 |
| Msa0026490 | Msa0869120 | 0.800031 | 1.744215e-48 | -8.615850e-47 |
| Msa0026490 | Msa0880140 | 0.809471 | 1.861985e-50 | -8.615850e-47 |
| Msa0026490 | Msa0899230 | 0.806886 | 6.615849e-50 | -8.615850e-47 |
| Msa0026490 | Msa0900180 | 0.800673 | 1.290974e-48 | -8.615850e-47 |
| Msa0026490 | Msa0908920 | -0.808865 | 2.510598e-50 | -8.615850e-47 |
| Msa0026490 | Msa0982270 | 0.811957 | 5.398673e-51 | -8.615850e-47 |
| Msa0026490 | Msa1000020 | 0.813490 | 2.493013e-51 | -8.615850e-47 |
| Msa0026490 | Msa1033410 | -0.800065 | 1.716652e-48 | -8.615850e-47 |
| Msa0026490 | Msa1043790 | 0.934710 | 2.646474e-96 | -8.615850e-47 |
| Msa0026490 | Msa1044420 | 0.813862 | 2.065053e-51 | -8.615850e-47 |
| Msa0026490 | Msa1073070 | -0.827073 | 1.913314e-54 | -8.615850e-47 |
| Msa0026490 | Msa1078710 | 0.818817 | 1.608234e-52 | -8.615850e-47 |
| Msa0026490 | Msa1082500 | 0.810623 | 1.051169e-50 | -8.615850e-47 |
| Msa0026490 | Msa1085300 | 0.807338 | 5.308706e-50 | -8.615850e-47 |
| Msa0026490 | Msa1124540 | -0.813235 | 2.836071e-51 | -8.615850e-47 |
| Msa0026490 | Msa1131220 | 0.808201 | 3.479760e-50 | -8.615850e-47 |
| Msa0026490 | Msa1133710 | 0.829945 | 3.874072e-55 | -8.615850e-47 |
| Msa0026490 | Msa1183100 | -0.849178 | 3.820125e-60 | -8.615850e-47 |
| Msa0026490 | Msa1202770 | -0.800737 | 1.252306e-48 | -8.615850e-47 |
| Msa0026490 | Msa1214880 | 0.805008 | 1.642459e-49 | -8.615850e-47 |
| Msa0026490 | Msa1217810 | 0.804342 | 2.262458e-49 | -8.615850e-47 |
| Msa0026490 | Msa1228160 | -0.800884 | 1.168842e-48 | -8.615850e-47 |
| Msa0026490 | Msa1228620 | -0.813740 | 2.196745e-51 | -8.615850e-47 |
| Msa0026490 | Msa1233500 | 0.809775 | 1.601250e-50 | -8.615850e-47 |
| Msa0026490 | Msa1305190 | 0.845705 | 3.437711e-59 | -8.615850e-47 |
| Msa0026490 | Msa1305340 | 0.843580 | 1.283741e-58 | -8.615850e-47 |
| Msa0026490 | Msa1307740 | 0.801904 | 7.226583e-49 | -8.615850e-47 |
| Msa0026490 | Msa1322190 | 0.831172 | 1.941149e-55 | -8.615850e-47 |
| Msa0026490 | Msa1359650 | -0.808975 | 2.377346e-50 | -8.615850e-47 |
| Msa0026490 | Msa1444250 | -0.805667 | 1.195126e-49 | -8.615850e-47 |
| Msa0026490 | Msa1451620 | -0.812365 | 4.397835e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0026490 | MtrunA17_Chr1g0153581 | 94.737 | 95 | 1 | 1 | 1 | 91 | 1 | 95 | 3.49e-61 | 181 |
| Msa0026490 | MtrunA17_Chr1g0153601 | 89.474 | 95 | 6 | 1 | 1 | 91 | 1 | 95 | 3.08e-55 | 166 |
| Msa0026490 | MtrunA17_Chr1g0153561 | 86.316 | 95 | 9 | 1 | 1 | 91 | 1 | 95 | 1.54e-54 | 164 |
| Msa0026490 | MtrunA17_Chr1g0153611 | 65.591 | 93 | 29 | 1 | 1 | 90 | 1 | 93 | 1.16e-38 | 124 |
| Msa0026490 | MtrunA17_Chr1g0153551 | 45.000 | 100 | 46 | 1 | 1 | 91 | 1 | 100 | 2.50e-19 | 75.5 |
| Msa0026490 | MtrunA17_Chr1g0153541 | 43.678 | 87 | 45 | 1 | 5 | 91 | 21 | 103 | 2.01e-16 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGATTAGTCCGTCTTTAT+TGG | 0.241517 | 1_1:+50323541 | None:intergenic |
| TATCTCCTTACCCGTGGTTT+TGG | 0.273881 | 1_1:-50323491 | Msa0026490:CDS |
| TGATCCATAGACACCCTCTT+TGG | 0.332546 | 1_1:+50323719 | None:intergenic |
| TATCCCAAAGAGGGTGTCTA+TGG | 0.383107 | 1_1:-50323723 | Msa0026490:CDS |
| GATCCATAGACACCCTCTTT+GGG | 0.423736 | 1_1:+50323720 | None:intergenic |
| CAACGATTGGCTCTCCATCC+AGG | 0.437823 | 1_1:+50323581 | None:intergenic |
| AGCTTTCATCAAACAACGAT+TGG | 0.498009 | 1_1:+50323568 | None:intergenic |
| TCTTGCCAAAACCACGGGTA+AGG | 0.519368 | 1_1:+50323486 | None:intergenic |
| CGATGGTCGTTACACAAAGA+AGG | 0.521782 | 1_1:-50323642 | Msa0026490:CDS |
| TAGGTTCTTACTACCCTGGA+TGG | 0.528156 | 1_1:-50323595 | Msa0026490:CDS |
| TTAGTTCTTGCCAAAACCAC+GGG | 0.528157 | 1_1:+50323481 | None:intergenic |
| TGGATCAAATCAACTTTGAA+AGG | 0.536831 | 1_1:-50323703 | Msa0026490:CDS |
| GATTTAGGTTCTTACTACCC+TGG | 0.543944 | 1_1:-50323599 | Msa0026490:CDS |
| AACGATTGGCTCTCCATCCA+GGG | 0.580860 | 1_1:+50323582 | None:intergenic |
| ATTAGTTCTTGCCAAAACCA+CGG | 0.592102 | 1_1:+50323480 | None:intergenic |
| GCCTGTGTTTATCCCAAAGA+GGG | 0.602626 | 1_1:-50323732 | Msa0026490:CDS |
| ATTGATTATCTCCTTACCCG+TGG | 0.619921 | 1_1:-50323497 | Msa0026490:CDS |
| AAAGCTGATGCCAATAAAGA+CGG | 0.628000 | 1_1:-50323551 | Msa0026490:CDS |
| TGCCTGTGTTTATCCCAAAG+AGG | 0.630941 | 1_1:-50323733 | Msa0026490:CDS |
| ACCCTCTTTGGGATAAACAC+AGG | 0.645492 | 1_1:+50323731 | None:intergenic |
| AGACGGACTAATCAGTGGCG+AGG | 0.659853 | 1_1:-50323534 | Msa0026490:CDS |
| AATAAAGACGGACTAATCAG+TGG | 0.668016 | 1_1:-50323539 | Msa0026490:CDS |
| AAGGCGGATATCAATAACGA+TGG | 0.697636 | 1_1:-50323659 | Msa0026490:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGGAACATTATAGAAAAAT+TGG | - | chr1_1:50323531-50323550 | Msa0026490:CDS | 20.0% |
| ! | CATTATAGAAAAATTGGAGA+AGG | - | chr1_1:50323537-50323556 | Msa0026490:CDS | 25.0% |
| !!! | AAAAAAGCTCTAAAGGATTT+AGG | - | chr1_1:50323601-50323620 | Msa0026490:CDS | 25.0% |
| TGGATCAAATCAACTTTGAA+AGG | - | chr1_1:50323512-50323531 | Msa0026490:CDS | 30.0% | |
| TATAGAAAAATTGGAGAAGG+CGG | - | chr1_1:50323540-50323559 | Msa0026490:CDS | 30.0% | |
| ! | GGAACTCAAAAAAGCTCTAA+AGG | - | chr1_1:50323594-50323613 | Msa0026490:CDS | 35.0% |
| AGCTTTCATCAAACAACGAT+TGG | + | chr1_1:50323650-50323669 | None:intergenic | 35.0% | |
| AAAGCTGATGCCAATAAAGA+CGG | - | chr1_1:50323664-50323683 | Msa0026490:CDS | 35.0% | |
| ! | ACTGATTAGTCCGTCTTTAT+TGG | + | chr1_1:50323677-50323696 | None:intergenic | 35.0% |
| !! | AATAAAGACGGACTAATCAG+TGG | - | chr1_1:50323676-50323695 | Msa0026490:CDS | 35.0% |
| AAGGCGGATATCAATAACGA+TGG | - | chr1_1:50323556-50323575 | Msa0026490:CDS | 40.0% | |
| ! | GATTTAGGTTCTTACTACCC+TGG | - | chr1_1:50323616-50323635 | Msa0026490:CDS | 40.0% |
| ATTGATTATCTCCTTACCCG+TGG | - | chr1_1:50323718-50323737 | Msa0026490:CDS | 40.0% | |
| ! | TTAGTTCTTGCCAAAACCAC+GGG | + | chr1_1:50323737-50323756 | None:intergenic | 40.0% |
| TGCCTGTGTTTATCCCAAAG+AGG | - | chr1_1:50323482-50323501 | Msa0026490:CDS | 45.0% | |
| GCCTGTGTTTATCCCAAAGA+GGG | - | chr1_1:50323483-50323502 | Msa0026490:CDS | 45.0% | |
| !! | ACCCTCTTTGGGATAAACAC+AGG | + | chr1_1:50323487-50323506 | None:intergenic | 45.0% |
| !! | TATCCCAAAGAGGGTGTCTA+TGG | - | chr1_1:50323492-50323511 | Msa0026490:CDS | 45.0% |
| GATCCATAGACACCCTCTTT+GGG | + | chr1_1:50323498-50323517 | None:intergenic | 45.0% | |
| TGATCCATAGACACCCTCTT+TGG | + | chr1_1:50323499-50323518 | None:intergenic | 45.0% | |
| CGATGGTCGTTACACAAAGA+AGG | - | chr1_1:50323573-50323592 | Msa0026490:CDS | 45.0% | |
| TAGGTTCTTACTACCCTGGA+TGG | - | chr1_1:50323620-50323639 | Msa0026490:CDS | 45.0% | |
| ! | TATCTCCTTACCCGTGGTTT+TGG | - | chr1_1:50323724-50323743 | Msa0026490:CDS | 45.0% |
| ! | AACGATTGGCTCTCCATCCA+GGG | + | chr1_1:50323636-50323655 | None:intergenic | 50.0% |
| TCTTGCCAAAACCACGGGTA+AGG | + | chr1_1:50323732-50323751 | None:intergenic | 50.0% | |
| ! | CAACGATTGGCTCTCCATCC+AGG | + | chr1_1:50323637-50323656 | None:intergenic | 55.0% |
| !! | AGACGGACTAATCAGTGGCG+AGG | - | chr1_1:50323681-50323700 | Msa0026490:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 50323481 | 50323756 | 50323481 | ID=Msa0026490;Name=Msa0026490 |
| chr1_1 | mRNA | 50323481 | 50323756 | 50323481 | ID=Msa0026490-mRNA-1;Parent=Msa0026490;Name=Msa0026490-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|91 |
| chr1_1 | exon | 50323481 | 50323756 | 50323481 | ID=Msa0026490-mRNA-1:exon:14630;Parent=Msa0026490-mRNA-1 |
| chr1_1 | CDS | 50323481 | 50323756 | 50323481 | ID=Msa0026490-mRNA-1:cds;Parent=Msa0026490-mRNA-1 |
| Gene Sequence |
| Protein sequence |
