AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0028800


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028800 MtrunA17_Chr1g0185331 94.515 237 13 0 1 237 1 237 5.67e-169 465
Msa0028800 MtrunA17_Chr7g0260011 56.977 258 88 8 1 236 1 257 1.48e-82 247
Msa0028800 MtrunA17_Chr1g0205211 55.140 107 36 2 141 235 180 286 1.02e-31 117
Msa0028800 MtrunA17_Chr1g0205211 44.348 115 50 6 30 136 12 120 2.27e-16 76.3
Msa0028800 MtrunA17_Chr3g0138651 47.826 115 36 3 144 234 280 394 1.83e-24 100
Msa0028800 MtrunA17_Chr1g0148411 48.214 112 37 3 144 234 204 315 5.99e-24 98.2
Msa0028800 MtrunA17_Chr2g0331941 47.321 112 48 4 125 226 240 350 5.84e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0028800 AT3G63210.1 40.377 265 125 11 2 236 1 262 1.33e-47 158
Msa0028800 AT3G22550.1 37.943 282 106 11 2 234 1 262 5.21e-45 152
Msa0028800 AT5G11460.1 28.464 267 100 8 52 230 44 307 1.18e-24 100
Msa0028800 AT2G25690.2 44.565 92 42 3 153 236 218 308 2.13e-17 80.1
Msa0028800 AT2G25690.1 44.565 92 42 3 153 236 218 308 2.13e-17 80.1
Msa0028800 AT5G20700.1 31.579 209 95 10 54 234 33 221 1.23e-11 63.2
Msa0028800 AT1G53885.1 37.500 64 40 0 171 234 19 82 4.70e-11 58.9
Msa0028800 AT1G53903.1 37.500 64 40 0 171 234 19 82 4.70e-11 58.9
Msa0028800 AT5G49120.1 48.889 45 23 0 193 237 67 111 8.34e-11 58.9

Find 31 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACATGTACTACAATATT+TGG 0.212149 1_1:-53854200 None:intergenic
ACTACAATACCATCTCTATT+TGG 0.228214 1_1:+53854461 Msa0028800:CDS
CAAAAGACATCTTCATTTAC+AGG 0.305275 1_1:+53855008 Msa0028800:CDS
GCTACAAACTTTGTTCCTAT+TGG 0.308716 1_1:-53854612 None:intergenic
CACACAAATAGAACTTAAAA+AGG 0.313516 1_1:-53855245 None:intergenic
TTCACCCATCAAACTTTGTT+TGG 0.353951 1_1:-53854417 None:intergenic
AAATGAAAATCTCTATAGTT+TGG 0.385441 1_1:-53854488 None:intergenic
ACCATTGGTCTTGGTCTTGT+TGG 0.396502 1_1:+53854659 Msa0028800:CDS
ACATGACTGAATAATATATA+TGG 0.397378 1_1:-53854081 None:intergenic
TTCCTATTGGTAATTGAGAA+AGG 0.412049 1_1:-53854599 None:intergenic
ATCTCTATTTGCAAGGTTGA+AGG 0.413036 1_1:-53855277 None:intergenic
ATCCTTTCTCAATTACCAAT+AGG 0.418780 1_1:+53854597 Msa0028800:CDS
AATTCAGTACACTTTCACTT+AGG 0.454438 1_1:+53854274 Msa0028800:five_prime_UTR
TGTTCTTAGTTTGAGTGAGA+TGG 0.477236 1_1:+53854811 Msa0028800:CDS
AGTCACCAAACAAAGTTTGA+TGG 0.487954 1_1:+53854412 Msa0028800:CDS
ATTGAAAAGTGTTTCACTTG+AGG 0.495448 1_1:-53854315 None:intergenic
AAAGTGTACTGAATTGTGTT+TGG 0.502524 1_1:-53854266 None:intergenic
TCAAACATGGAAGGTGGTAG+AGG 0.507899 1_1:-53854776 None:intergenic
GGAACAAAGTTTGTAGCTCA+AGG 0.520520 1_1:+53854618 Msa0028800:CDS
TACACATGTGTGATATCTCA+TGG 0.544339 1_1:+53854848 Msa0028800:CDS
CTTGCAAATAGAGATAAAAG+AGG 0.555159 1_1:+53855285 Msa0028800:three_prime_UTR
GTCACCAAACAAAGTTTGAT+GGG 0.561348 1_1:+53854413 Msa0028800:CDS
TTTGTGTCAAGAATTGATGT+TGG 0.576565 1_1:-53854554 None:intergenic
TCTATTTGCAAGGTTGAAGG+AGG 0.594942 1_1:-53855274 None:intergenic
TATATTTGATAATTGTGTTG+TGG 0.597437 1_1:+53854892 Msa0028800:CDS
GAACAAAGTTTGTAGCTCAA+GGG 0.606947 1_1:+53854619 Msa0028800:CDS
AACATGGAAGGTGGTAGAGG+AGG 0.614545 1_1:-53854773 None:intergenic
TTTGGTGAACCAAATAGAGA+TGG 0.621510 1_1:-53854470 None:intergenic
ACCAACAAGACCAAGACCAA+TGG 0.643171 1_1:-53854660 None:intergenic
TGTGACTCAAACATGGAAGG+TGG 0.643194 1_1:-53854782 None:intergenic
AGAATGTAGACACAAAGAGA+TGG 0.662022 1_1:+53855162 Msa0028800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATATATGGCTTTTAA+AGG - chr1_1:53854074-53854093 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTTGCAG+AGG + chr1_1:53855118-53855137 Msa0028800:intron 15.0%
!!! ATAATATATATGGCTTTTAA+AGG - chr1_1:53854074-53854093 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTTGCAG+AGG + chr1_1:53855118-53855137 Msa0028800:intron 15.0%
!! ACATGACTGAATAATATATA+TGG - chr1_1:53854084-53854103 None:intergenic 20.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr1_1:53854449-53854468 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr1_1:53854491-53854510 None:intergenic 20.0%
!! AGTAATAGAAAAATTCTCTT+TGG + chr1_1:53854728-53854747 Msa0028800:CDS 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr1_1:53854880-53854899 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr1_1:53854881-53854900 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr1_1:53854892-53854911 Msa0028800:CDS 20.0%
!! ACATGACTGAATAATATATA+TGG - chr1_1:53854084-53854103 None:intergenic 20.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr1_1:53854449-53854468 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr1_1:53854491-53854510 None:intergenic 20.0%
!! AGTAATAGAAAAATTCTCTT+TGG + chr1_1:53854728-53854747 Msa0028800:CDS 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr1_1:53854880-53854899 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr1_1:53854881-53854900 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr1_1:53854892-53854911 Msa0028800:CDS 20.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr1_1:53854134-53854153 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr1_1:53854644-53854663 Msa0028800:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr1_1:53854701-53854720 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr1_1:53855248-53855267 None:intergenic 25.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr1_1:53854134-53854153 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr1_1:53854644-53854663 Msa0028800:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr1_1:53854701-53854720 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr1_1:53855248-53855267 None:intergenic 25.0%
! AAGAAGGAAACTTTTGACAT+GGG - chr1_1:53854118-53854137 None:intergenic 30.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr1_1:53854119-53854138 None:intergenic 30.0%
GGTACATGTACTACAATATT+TGG - chr1_1:53854203-53854222 None:intergenic 30.0%
! GAAAAAGCATGATTTAGTAG+TGG - chr1_1:53854224-53854243 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr1_1:53854269-53854288 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr1_1:53854274-53854293 Msa0028800:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr1_1:53854318-53854337 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr1_1:53854461-53854480 Msa0028800:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr1_1:53854557-53854576 None:intergenic 30.0%
! TGAGAAAGGATTTTCAAATG+AGG - chr1_1:53854588-53854607 None:intergenic 30.0%
ATCCTTTCTCAATTACCAAT+AGG + chr1_1:53854597-53854616 Msa0028800:CDS 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr1_1:53854602-53854621 None:intergenic 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr1_1:53854792-53854811 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr1_1:53854875-53854894 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr1_1:53854937-53854956 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr1_1:53854947-53854966 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr1_1:53855008-53855027 Msa0028800:CDS 30.0%
TACTTGAGCAAAGCTTATTA+AGG + chr1_1:53855076-53855095 Msa0028800:intron 30.0%
!! ATTAAGGAGCATTAACTTGA+TGG + chr1_1:53855092-53855111 Msa0028800:intron 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr1_1:53855287-53855306 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr1_1:53855285-53855304 Msa0028800:three_prime_UTR 30.0%
! AAGAAGGAAACTTTTGACAT+GGG - chr1_1:53854118-53854137 None:intergenic 30.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr1_1:53854119-53854138 None:intergenic 30.0%
GGTACATGTACTACAATATT+TGG - chr1_1:53854203-53854222 None:intergenic 30.0%
! GAAAAAGCATGATTTAGTAG+TGG - chr1_1:53854224-53854243 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr1_1:53854269-53854288 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr1_1:53854274-53854293 Msa0028800:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr1_1:53854318-53854337 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr1_1:53854461-53854480 Msa0028800:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr1_1:53854557-53854576 None:intergenic 30.0%
! TGAGAAAGGATTTTCAAATG+AGG - chr1_1:53854588-53854607 None:intergenic 30.0%
ATCCTTTCTCAATTACCAAT+AGG + chr1_1:53854597-53854616 Msa0028800:CDS 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr1_1:53854602-53854621 None:intergenic 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr1_1:53854792-53854811 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr1_1:53854875-53854894 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr1_1:53854937-53854956 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr1_1:53854947-53854966 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr1_1:53855008-53855027 Msa0028800:CDS 30.0%
TACTTGAGCAAAGCTTATTA+AGG + chr1_1:53855076-53855095 Msa0028800:intron 30.0%
!! ATTAAGGAGCATTAACTTGA+TGG + chr1_1:53855092-53855111 Msa0028800:intron 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr1_1:53855287-53855306 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr1_1:53855285-53855304 Msa0028800:three_prime_UTR 30.0%
AGTCACCAAACAAAGTTTGA+TGG + chr1_1:53854412-53854431 Msa0028800:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr1_1:53854413-53854432 Msa0028800:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr1_1:53854420-53854439 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr1_1:53854473-53854492 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr1_1:53854512-53854531 Msa0028800:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr1_1:53854519-53854538 None:intergenic 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr1_1:53854615-53854634 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr1_1:53854619-53854638 Msa0028800:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr1_1:53854650-53854669 Msa0028800:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr1_1:53854788-53854807 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr1_1:53854811-53854830 Msa0028800:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr1_1:53854848-53854867 Msa0028800:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr1_1:53854938-53854957 None:intergenic 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr1_1:53855162-53855181 Msa0028800:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr1_1:53855280-53855299 None:intergenic 35.0%
TGTGCATGAAAATCAAACGT+TGG - chr1_1:53854030-53854049 None:intergenic 35.0%
AGTCACCAAACAAAGTTTGA+TGG + chr1_1:53854412-53854431 Msa0028800:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr1_1:53854413-53854432 Msa0028800:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr1_1:53854420-53854439 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr1_1:53854473-53854492 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr1_1:53854512-53854531 Msa0028800:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr1_1:53854519-53854538 None:intergenic 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr1_1:53854615-53854634 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr1_1:53854619-53854638 Msa0028800:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr1_1:53854650-53854669 Msa0028800:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr1_1:53854788-53854807 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr1_1:53854811-53854830 Msa0028800:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr1_1:53854848-53854867 Msa0028800:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr1_1:53854938-53854957 None:intergenic 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr1_1:53855162-53855181 Msa0028800:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr1_1:53855280-53855299 None:intergenic 35.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr1_1:53854618-53854637 Msa0028800:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr1_1:53855277-53855296 None:intergenic 40.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr1_1:53854618-53854637 Msa0028800:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr1_1:53855277-53855296 None:intergenic 40.0%
!! ACCATTGGTCTTGGTCTTGT+TGG + chr1_1:53854659-53854678 Msa0028800:CDS 45.0%
ACCAACAAGACCAAGACCAA+TGG - chr1_1:53854663-53854682 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr1_1:53854779-53854798 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr1_1:53854785-53854804 None:intergenic 45.0%
!! ACCATTGGTCTTGGTCTTGT+TGG + chr1_1:53854659-53854678 Msa0028800:CDS 45.0%
ACCAACAAGACCAAGACCAA+TGG - chr1_1:53854663-53854682 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr1_1:53854779-53854798 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr1_1:53854785-53854804 None:intergenic 45.0%
AACATGGAAGGTGGTAGAGG+AGG - chr1_1:53854776-53854795 None:intergenic 50.0%
AACATGGAAGGTGGTAGAGG+AGG - chr1_1:53854776-53854795 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 53854029 53855395 53854029 ID=Msa0028800;Name=Msa0028800
chr1_1 mRNA 53854029 53855395 53854029 ID=Msa0028800-mRNA-1;Parent=Msa0028800;Name=Msa0028800-mRNA-1;_AED=0.04;_eAED=0.04;_QI=357|1|1|1|1|1|2|188|237
chr1_1 exon 53854029 53855029 53854029 ID=Msa0028800-mRNA-1:exon:15888;Parent=Msa0028800-mRNA-1
chr1_1 exon 53855138 53855395 53855138 ID=Msa0028800-mRNA-1:exon:15889;Parent=Msa0028800-mRNA-1
chr1_1 five_prime_UTR 53854029 53854385 53854029 ID=Msa0028800-mRNA-1:five_prime_utr;Parent=Msa0028800-mRNA-1
chr1_1 CDS 53854386 53855029 53854386 ID=Msa0028800-mRNA-1:cds;Parent=Msa0028800-mRNA-1
chr1_1 CDS 53855138 53855207 53855138 ID=Msa0028800-mRNA-1:cds;Parent=Msa0028800-mRNA-1
chr1_1 three_prime_UTR 53855208 53855395 53855208 ID=Msa0028800-mRNA-1:three_prime_utr;Parent=Msa0028800-mRNA-1
Gene Sequence

>Msa0028800

ATGCTACTCAGAAACAGATCAAGAGCAGTCACCAAACAAAGTTTGATGGGTGAACAATCACCAAATCAAAATTTCACTACAATACCATCTCTATTTGGTTCACCAAACTATAGAGATTTTCATTTCACTTCCAAAAAATCTCTCACTGGAACTGAAGCTTTGATAAGTCCAACATCAATTCTTGACACAAAAGCGTTAACCTCATTTGAAAATCCTTTCTCAATTACCAATAGGAACAAAGTTTGTAGCTCAAGGGACAAAGTAGATTCAAAAACCATTGGTCTTGGTCTTGTTGGTGTTCTAAAAGATGAACCTTTTCATCAAAATTCTGATCATAAACTAAGTAATAGAAAAATTCTCTTTGGAACTGAGCTTAGAGTGAAAATTCCTCCTCTACCACCTTCCATGTTTGAGTCACAAAAAAGTGTTCTTAGTTTGAGTGAGATGGAACTTTCTGAAGAATACACATGTGTGATATCTCATGGACCAAACCCAAAAACAACTCATATATTTGATAATTGTGTTGTGGAAGAAAGTTACTATTCTGTCCCTAATAGTCCTCACTCTTTTTCACTGAATTTTCTTAGCTTTTGTTACACTTGCAAAAATCATCTTGAGCAAACAAAAGACATCTTCATTTACAGAGGAGAAAAAGCTTTTTGCAGTCAAGAATGTAGACACAAAGAGATGGTGTTAGATGAAGCAGAAAATTAA

Protein sequence

>Msa0028800

MLLRNRSRAVTKQSLMGEQSPNQNFTTIPSLFGSPNYRDFHFTSKKSLTGTEALISPTSILDTKALTSFENPFSITNRNKVCSSRDKVDSKTIGLGLVGVLKDEPFHQNSDHKLSNRKILFGTELRVKIPPLPPSMFESQKSVLSLSEMELSEEYTCVISHGPNPKTTHIFDNCVVEESYYSVPNSPHSFSLNFLSFCYTCKNHLEQTKDIFIYRGEKAFCSQECRHKEMVLDEAEN*