AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0791630


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0791630 MtrunA17_Chr1g0185331 93.305 239 14 1 1 239 1 237 3.45e-166 458
Msa0791630 MtrunA17_Chr7g0260011 56.371 259 90 8 1 238 1 257 9.03e-82 245
Msa0791630 MtrunA17_Chr1g0205211 55.140 107 36 2 143 237 180 286 1.06e-31 117
Msa0791630 MtrunA17_Chr1g0205211 43.590 117 50 7 30 138 12 120 1.15e-14 71.6
Msa0791630 MtrunA17_Chr3g0138651 47.826 115 36 3 146 236 280 394 2.01e-24 100
Msa0791630 MtrunA17_Chr1g0148411 48.214 112 37 3 146 236 204 315 6.56e-24 97.8
Msa0791630 MtrunA17_Chr2g0331941 47.321 112 48 4 127 228 240 350 6.12e-21 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0791630 AT3G63210.1 40.075 267 125 11 2 238 1 262 2.64e-47 157
Msa0791630 AT3G22550.1 37.456 283 108 11 2 236 1 262 3.34e-44 149
Msa0791630 AT5G11460.1 28.253 269 100 9 52 232 44 307 1.20e-22 94.7
Msa0791630 AT2G25690.2 44.565 92 42 3 155 238 218 308 2.33e-17 80.1
Msa0791630 AT2G25690.1 44.565 92 42 3 155 238 218 308 2.33e-17 80.1
Msa0791630 AT5G20700.1 32.227 211 93 11 54 236 33 221 2.82e-11 62.0
Msa0791630 AT1G53885.1 37.500 64 40 0 173 236 19 82 4.64e-11 59.3
Msa0791630 AT1G53903.1 37.500 64 40 0 173 236 19 82 4.64e-11 59.3
Msa0791630 AT5G49120.1 48.889 45 23 0 195 239 67 111 9.67e-11 58.9

Find 33 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTACATGTACTACAATATT+TGG 0.212149 5_3:-2684437 None:intergenic
ACTACAATACCATCTCTATT+TGG 0.228214 5_3:+2684695 Msa0791630:CDS
CAAAAGACATCTTCATTTAC+AGG 0.305275 5_3:+2685248 Msa0791630:CDS
GCTACAAACTTTGTTCCTAT+TGG 0.308716 5_3:-2684846 None:intergenic
CACACAAATAGAACTTAAAA+AGG 0.313516 5_3:-2685483 None:intergenic
GGTCTTGGTCTTGGTCTTGT+TGG 0.321698 5_3:+2684899 Msa0791630:CDS
TTCACCCATCAAACTTTGTT+TGG 0.353951 5_3:-2684651 None:intergenic
AAAACCATTGGTCTTGGTCT+TGG 0.366469 5_3:+2684890 Msa0791630:CDS
AAATGAAAATCTCTATAGTT+TGG 0.385441 5_3:-2684722 None:intergenic
ACATGACTGAATAATATATA+TGG 0.397378 5_3:-2684319 None:intergenic
TTCCTATTGGTAATTGAGAA+AGG 0.411279 5_3:-2684833 None:intergenic
ATCTCTATTTGCAAGGTTGA+AGG 0.413036 5_3:-2685515 None:intergenic
ACCCTTTCTCAATTACCAAT+AGG 0.433358 5_3:+2684831 Msa0791630:CDS
AATTCAGTACACTTTCACTT+AGG 0.454438 5_3:+2684511 Msa0791630:five_prime_UTR
TGTTCTTAGTTTGAGTGAGA+TGG 0.477236 5_3:+2685051 Msa0791630:CDS
AGTCACCAAACAAAGTTTGA+TGG 0.487954 5_3:+2684646 Msa0791630:CDS
TCCTATTGGTAATTGAGAAA+GGG 0.489680 5_3:-2684832 None:intergenic
ATTGAAAAGTGTTTCACTTG+AGG 0.495448 5_3:-2684552 None:intergenic
AAAGTGTACTGAATTGTGTT+TGG 0.502524 5_3:-2684503 None:intergenic
TCAAACATGGAAGGTGGTAG+AGG 0.507899 5_3:-2685016 None:intergenic
GGAACAAAGTTTGTAGCTCA+AGG 0.520520 5_3:+2684852 Msa0791630:CDS
TACACATGTGTGATATCTCA+TGG 0.544339 5_3:+2685088 Msa0791630:CDS
CTTGCAAATAGAGATAAAAG+AGG 0.555159 5_3:+2685523 Msa0791630:three_prime_UTR
GTCACCAAACAAAGTTTGAT+GGG 0.561348 5_3:+2684647 Msa0791630:CDS
TTTGTGTCAAGAATTGATGT+TGG 0.576565 5_3:-2684788 None:intergenic
TCTATTTGCAAGGTTGAAGG+AGG 0.594942 5_3:-2685512 None:intergenic
TATATTTGATAATTGTGTTG+TGG 0.597437 5_3:+2685132 Msa0791630:CDS
GAACAAAGTTTGTAGCTCAA+GGG 0.606947 5_3:+2684853 Msa0791630:CDS
AACATGGAAGGTGGTAGAGG+AGG 0.614545 5_3:-2685013 None:intergenic
TTTGGTGAACCAAATAGAGA+TGG 0.621510 5_3:-2684704 None:intergenic
TGTGACTCAAACATGGAAGG+TGG 0.643194 5_3:-2685022 None:intergenic
AAGACCAAGACCAAGACCAA+TGG 0.659900 5_3:-2684894 None:intergenic
AGAATGTAGACACAAAGAGA+TGG 0.662022 5_3:+2685400 Msa0791630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATATATGGCTTTTAA+AGG - chr5_3:2684312-2684331 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTTGCAG+AGG + chr5_3:2685356-2685375 Msa0791630:intron 15.0%
!!! ATAATATATATGGCTTTTAA+AGG - chr5_3:2684312-2684331 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTTTGCAG+AGG + chr5_3:2685356-2685375 Msa0791630:intron 15.0%
!! ACATGACTGAATAATATATA+TGG - chr5_3:2684322-2684341 None:intergenic 20.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr5_3:2684683-2684702 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr5_3:2684725-2684744 None:intergenic 20.0%
!! AGTAATAGAAAAATTCTCTT+TGG + chr5_3:2684968-2684987 Msa0791630:CDS 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr5_3:2685120-2685139 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr5_3:2685121-2685140 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr5_3:2685132-2685151 Msa0791630:CDS 20.0%
!! ACATGACTGAATAATATATA+TGG - chr5_3:2684322-2684341 None:intergenic 20.0%
!!! ATTGTAGTGAAATTTTGATT+TGG - chr5_3:2684683-2684702 None:intergenic 20.0%
!! AAATGAAAATCTCTATAGTT+TGG - chr5_3:2684725-2684744 None:intergenic 20.0%
!! AGTAATAGAAAAATTCTCTT+TGG + chr5_3:2684968-2684987 Msa0791630:CDS 20.0%
!!! CAAATATATGAGTTGTTTTT+GGG - chr5_3:2685120-2685139 None:intergenic 20.0%
!!! TCAAATATATGAGTTGTTTT+TGG - chr5_3:2685121-2685140 None:intergenic 20.0%
!!! TATATTTGATAATTGTGTTG+TGG + chr5_3:2685132-2685151 Msa0791630:CDS 20.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr5_3:2684371-2684390 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr5_3:2684878-2684897 Msa0791630:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr5_3:2684941-2684960 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr5_3:2685486-2685505 None:intergenic 25.0%
! AAAGAAAGAGAAGAAAAAGA+AGG - chr5_3:2684371-2684390 None:intergenic 25.0%
! AAAGTAGATTCAAAAACCAT+TGG + chr5_3:2684878-2684897 Msa0791630:CDS 25.0%
!!! TGATCAGAATTTTGATGAAA+AGG - chr5_3:2684941-2684960 None:intergenic 25.0%
! CACACAAATAGAACTTAAAA+AGG - chr5_3:2685486-2685505 None:intergenic 25.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr5_3:2684356-2684375 None:intergenic 30.0%
GGTACATGTACTACAATATT+TGG - chr5_3:2684440-2684459 None:intergenic 30.0%
! GAAAAAGCATGATTTAGTAG+TGG - chr5_3:2684461-2684480 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr5_3:2684506-2684525 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr5_3:2684511-2684530 Msa0791630:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr5_3:2684555-2684574 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr5_3:2684695-2684714 Msa0791630:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr5_3:2684791-2684810 None:intergenic 30.0%
! TCCTATTGGTAATTGAGAAA+GGG - chr5_3:2684835-2684854 None:intergenic 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr5_3:2684836-2684855 None:intergenic 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr5_3:2685032-2685051 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr5_3:2685115-2685134 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr5_3:2685177-2685196 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr5_3:2685187-2685206 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr5_3:2685248-2685267 Msa0791630:CDS 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr5_3:2685525-2685544 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr5_3:2685523-2685542 Msa0791630:three_prime_UTR 30.0%
! AAAGAAGGAAACTTTTGACA+TGG - chr5_3:2684356-2684375 None:intergenic 30.0%
GGTACATGTACTACAATATT+TGG - chr5_3:2684440-2684459 None:intergenic 30.0%
! GAAAAAGCATGATTTAGTAG+TGG - chr5_3:2684461-2684480 None:intergenic 30.0%
AAAGTGTACTGAATTGTGTT+TGG - chr5_3:2684506-2684525 None:intergenic 30.0%
AATTCAGTACACTTTCACTT+AGG + chr5_3:2684511-2684530 Msa0791630:five_prime_UTR 30.0%
!! ATTGAAAAGTGTTTCACTTG+AGG - chr5_3:2684555-2684574 None:intergenic 30.0%
ACTACAATACCATCTCTATT+TGG + chr5_3:2684695-2684714 Msa0791630:CDS 30.0%
TTTGTGTCAAGAATTGATGT+TGG - chr5_3:2684791-2684810 None:intergenic 30.0%
! TCCTATTGGTAATTGAGAAA+GGG - chr5_3:2684835-2684854 None:intergenic 30.0%
! TTCCTATTGGTAATTGAGAA+AGG - chr5_3:2684836-2684855 None:intergenic 30.0%
!!! ACTTTTTTGTGACTCAAACA+TGG - chr5_3:2685032-2685051 None:intergenic 30.0%
!!! ATATGAGTTGTTTTTGGGTT+TGG - chr5_3:2685115-2685134 None:intergenic 30.0%
AAAAAGAGTGAGGACTATTA+GGG - chr5_3:2685177-2685196 None:intergenic 30.0%
AAATTCAGTGAAAAAGAGTG+AGG - chr5_3:2685187-2685206 None:intergenic 30.0%
CAAAAGACATCTTCATTTAC+AGG + chr5_3:2685248-2685267 Msa0791630:CDS 30.0%
! CTCTTTTATCTCTATTTGCA+AGG - chr5_3:2685525-2685544 None:intergenic 30.0%
CTTGCAAATAGAGATAAAAG+AGG + chr5_3:2685523-2685542 Msa0791630:three_prime_UTR 30.0%
AGTCACCAAACAAAGTTTGA+TGG + chr5_3:2684646-2684665 Msa0791630:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr5_3:2684647-2684666 Msa0791630:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr5_3:2684654-2684673 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr5_3:2684707-2684726 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr5_3:2684746-2684765 Msa0791630:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr5_3:2684753-2684772 None:intergenic 35.0%
! TGAGAAAGGGTTTTCAAATG+AGG - chr5_3:2684822-2684841 None:intergenic 35.0%
ACCCTTTCTCAATTACCAAT+AGG + chr5_3:2684831-2684850 Msa0791630:CDS 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr5_3:2684849-2684868 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr5_3:2684853-2684872 Msa0791630:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr5_3:2684884-2684903 Msa0791630:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr5_3:2685028-2685047 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr5_3:2685051-2685070 Msa0791630:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr5_3:2685088-2685107 Msa0791630:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr5_3:2685178-2685197 None:intergenic 35.0%
!! TACTTGAGCAAAGCTTGTTA+AGG + chr5_3:2685316-2685335 Msa0791630:intron 35.0%
!! GTTAAGGAGCATTAACTTGA+TGG + chr5_3:2685332-2685351 Msa0791630:intron 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr5_3:2685400-2685419 Msa0791630:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr5_3:2685518-2685537 None:intergenic 35.0%
TGTGCATGAAAATCAAACGT+TGG - chr5_3:2684268-2684287 None:intergenic 35.0%
AGTCACCAAACAAAGTTTGA+TGG + chr5_3:2684646-2684665 Msa0791630:CDS 35.0%
GTCACCAAACAAAGTTTGAT+GGG + chr5_3:2684647-2684666 Msa0791630:CDS 35.0%
TTCACCCATCAAACTTTGTT+TGG - chr5_3:2684654-2684673 None:intergenic 35.0%
TTTGGTGAACCAAATAGAGA+TGG - chr5_3:2684707-2684726 None:intergenic 35.0%
ACTTCCAAAAAATCTCTCAC+TGG + chr5_3:2684746-2684765 Msa0791630:CDS 35.0%
!!! AGTTCCAGTGAGAGATTTTT+TGG - chr5_3:2684753-2684772 None:intergenic 35.0%
! TGAGAAAGGGTTTTCAAATG+AGG - chr5_3:2684822-2684841 None:intergenic 35.0%
ACCCTTTCTCAATTACCAAT+AGG + chr5_3:2684831-2684850 Msa0791630:CDS 35.0%
GCTACAAACTTTGTTCCTAT+TGG - chr5_3:2684849-2684868 None:intergenic 35.0%
GAACAAAGTTTGTAGCTCAA+GGG + chr5_3:2684853-2684872 Msa0791630:CDS 35.0%
! GATTCAAAAACCATTGGTCT+TGG + chr5_3:2684884-2684903 Msa0791630:CDS 35.0%
! TTTTGTGACTCAAACATGGA+AGG - chr5_3:2685028-2685047 None:intergenic 35.0%
! TGTTCTTAGTTTGAGTGAGA+TGG + chr5_3:2685051-2685070 Msa0791630:CDS 35.0%
TACACATGTGTGATATCTCA+TGG + chr5_3:2685088-2685107 Msa0791630:CDS 35.0%
GAAAAAGAGTGAGGACTATT+AGG - chr5_3:2685178-2685197 None:intergenic 35.0%
!! TACTTGAGCAAAGCTTGTTA+AGG + chr5_3:2685316-2685335 Msa0791630:intron 35.0%
!! GTTAAGGAGCATTAACTTGA+TGG + chr5_3:2685332-2685351 Msa0791630:intron 35.0%
AGAATGTAGACACAAAGAGA+TGG + chr5_3:2685400-2685419 Msa0791630:CDS 35.0%
ATCTCTATTTGCAAGGTTGA+AGG - chr5_3:2685518-2685537 None:intergenic 35.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr5_3:2684852-2684871 Msa0791630:CDS 40.0%
!! AAAACCATTGGTCTTGGTCT+TGG + chr5_3:2684890-2684909 Msa0791630:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr5_3:2685515-2685534 None:intergenic 40.0%
GGAACAAAGTTTGTAGCTCA+AGG + chr5_3:2684852-2684871 Msa0791630:CDS 40.0%
!! AAAACCATTGGTCTTGGTCT+TGG + chr5_3:2684890-2684909 Msa0791630:CDS 40.0%
TCTATTTGCAAGGTTGAAGG+AGG - chr5_3:2685515-2685534 None:intergenic 40.0%
AAGACCAAGACCAAGACCAA+TGG - chr5_3:2684897-2684916 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr5_3:2685019-2685038 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr5_3:2685025-2685044 None:intergenic 45.0%
AAGACCAAGACCAAGACCAA+TGG - chr5_3:2684897-2684916 None:intergenic 45.0%
TCAAACATGGAAGGTGGTAG+AGG - chr5_3:2685019-2685038 None:intergenic 45.0%
TGTGACTCAAACATGGAAGG+TGG - chr5_3:2685025-2685044 None:intergenic 45.0%
!! GGTCTTGGTCTTGGTCTTGT+TGG + chr5_3:2684899-2684918 Msa0791630:CDS 50.0%
AACATGGAAGGTGGTAGAGG+AGG - chr5_3:2685016-2685035 None:intergenic 50.0%
!! GGTCTTGGTCTTGGTCTTGT+TGG + chr5_3:2684899-2684918 Msa0791630:CDS 50.0%
AACATGGAAGGTGGTAGAGG+AGG - chr5_3:2685016-2685035 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 2684267 2685633 2684267 ID=Msa0791630;Name=Msa0791630
chr5_3 mRNA 2684267 2685633 2684267 ID=Msa0791630-mRNA-1;Parent=Msa0791630;Name=Msa0791630-mRNA-1;_AED=0.01;_eAED=0.01;_QI=353|1|1|1|1|1|2|188|239
chr5_3 exon 2684267 2685269 2684267 ID=Msa0791630-mRNA-1:exon:1113;Parent=Msa0791630-mRNA-1
chr5_3 exon 2685376 2685633 2685376 ID=Msa0791630-mRNA-1:exon:1114;Parent=Msa0791630-mRNA-1
chr5_3 five_prime_UTR 2684267 2684619 2684267 ID=Msa0791630-mRNA-1:five_prime_utr;Parent=Msa0791630-mRNA-1
chr5_3 CDS 2684620 2685269 2684620 ID=Msa0791630-mRNA-1:cds;Parent=Msa0791630-mRNA-1
chr5_3 CDS 2685376 2685445 2685376 ID=Msa0791630-mRNA-1:cds;Parent=Msa0791630-mRNA-1
chr5_3 three_prime_UTR 2685446 2685633 2685446 ID=Msa0791630-mRNA-1:three_prime_utr;Parent=Msa0791630-mRNA-1
Gene Sequence

>Msa0791630

ATGCTACTCAGAAACAGATCAAGAGCAGTCACCAAACAAAGTTTGATGGGTGAACAATCACCAAATCAAAATTTCACTACAATACCATCTCTATTTGGTTCACCAAACTATAGAGATTTTCATTTCACTTCCAAAAAATCTCTCACTGGAACTGAAGCTTTGATAAGTCCAACATCAATTCTTGACACAAAAGCGTTAGCCTCATTTGAAAACCCTTTCTCAATTACCAATAGGAACAAAGTTTGTAGCTCAAGGGACAAAGTAGATTCAAAAACCATTGGTCTTGGTCTTGGTCTTGTTGGTGTTCTAAAAGATGAACCTTTTCATCAAAATTCTGATCATAAACTAAGTAATAGAAAAATTCTCTTTGGAACTGAGCTTAGAGTGAAAATTCCTCCTCTACCACCTTCCATGTTTGAGTCACAAAAAAGTGTTCTTAGTTTGAGTGAGATGGAACTTTCTGAAGAATACACATGTGTGATATCTCATGGACCAAACCCAAAAACAACTCATATATTTGATAATTGTGTTGTGGAAGAAAGTTACTATTCTGTCCCTAATAGTCCTCACTCTTTTTCACTGAATTTTCTTAGCTTTTGTTACACTTGCAAAAATCATCTTGAGCAAACAAAAGACATCTTCATTTACAGAGGAGAAAAAGCTTTTTGCAGTCAAGAATGTAGACACAAAGAGATGGTGTTAGATGAAGCAGAAAATTAA

Protein sequence

>Msa0791630

MLLRNRSRAVTKQSLMGEQSPNQNFTTIPSLFGSPNYRDFHFTSKKSLTGTEALISPTSILDTKALASFENPFSITNRNKVCSSRDKVDSKTIGLGLGLVGVLKDEPFHQNSDHKLSNRKILFGTELRVKIPPLPPSMFESQKSVLSLSEMELSEEYTCVISHGPNPKTTHIFDNCVVEESYYSVPNSPHSFSLNFLSFCYTCKNHLEQTKDIFIYRGEKAFCSQECRHKEMVLDEAEN*