AlfalfaGEDB | Msa0148200

M. sativa cultivar zhongmu-4 / Msa0148200

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0010035 GO:0010556 GO:0010558 GO:0010605 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2001141
PFAM: PF12165.11

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148200	XP_013465876.1	99.242	264	1	1	1	264	1	263	0.0	543

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148200	sp\|Q5XEM9\|ALFL5_ARATH	71.591	264	71	2	1	264	1	260	5.97e-123	353

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148200	A0A072VES1	99.242	264	1	1	1	264	1	263	0.0	543

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TFs/TRs:

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Gene ID	Type	Classification
Msa0148200	TF	Alfin-like

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0028800	Msa0148200	0.807028	6.173992e-50	-8.615850e-47
Msa0099470	Msa0148200	0.848489	5.934667e-60	-8.615850e-47
Msa0099590	Msa0148200	0.953658	1.686142e-111	-8.615850e-47
Msa0148200	Msa0791630	0.821605	3.694678e-53	-8.615850e-47
Msa0148200	Msa1043550	0.970050	4.954481e-131	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148200	MtrunA17_Chr1g0150821	99.242	264	1	1	1	264	1	263	0.0	543
Msa0148200	MtrunA17_Chr8g0343441	78.378	259	45	3	8	264	7	256	1.90e-130	369
Msa0148200	MtrunA17_Chr2g0300241	74.809	262	49	3	5	264	7	253	3.98e-125	356
Msa0148200	MtrunA17_Chr4g0076771	70.588	255	64	3	10	263	10	254	2.95e-113	326
Msa0148200	MtrunA17_Chr2g0329821	72.441	254	60	3	11	263	6	250	5.75e-111	320
Msa0148200	MtrunA17_Chr4g0006381	62.500	256	77	4	9	264	6	242	4.22e-94	277
Msa0148200	MtrunA17_Chr7g0242511	62.500	256	77	6	9	264	6	242	1.67e-92	273

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148200	AT5G20510.1	71.591	264	71	2	1	264	1	260	6.07e-124	353
Msa0148200	AT3G42790.1	72.348	264	57	3	1	263	1	249	1.08e-122	350
Msa0148200	AT2G02470.1	70.196	255	69	3	10	264	9	256	9.23e-111	320
Msa0148200	AT2G02470.2	67.578	256	65	3	10	264	9	247	2.32e-109	316
Msa0148200	AT5G26210.1	78.077	260	49	1	4	263	3	254	2.14e-108	314
Msa0148200	AT1G14510.1	67.063	252	71	2	10	261	9	248	6.38e-107	310
Msa0148200	AT5G20510.2	71.364	220	59	2	45	264	4	219	4.27e-98	286
Msa0148200	AT5G05610.2	61.961	255	76	5	9	263	7	240	1.38e-97	286
Msa0148200	AT5G05610.1	61.961	255	76	5	9	263	7	240	1.38e-97	286
Msa0148200	AT3G11200.1	61.977	263	82	3	1	263	1	245	1.32e-96	283

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Find 89 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGCGTGAAGATTACTC+CGG	0.732353	1_4:+8453021	Msa0148200:CDS
AACACGGAGAGACTTTGTGT+GGG	0.622939	1_4:+8452927	Msa0148200:CDS
AAGACTTCTTCGACAGTACG+AGG	0.536014	1_4:-8448973	None:intergenic
AAGATAAGAGAGTGAGATTG+TGG	0.422672	1_4:-8448886	None:intergenic
AAGGAAAATCTTTGCCTTTA+TGG	0.094878	1_4:+8451078	Msa0148200:CDS
AAGTTGTTACAGGGTCTGGC+AGG	0.518216	1_4:+8452233	Msa0148200:CDS
AATGCTGGCTCTGGAAGCTC+AGG	0.453734	1_4:-8451150	None:intergenic
AATTCGGAACAATTCGATAG+TGG	0.501907	1_4:-8453244	None:intergenic
ACAACAACCATGGACGTTGT+AGG	0.512436	1_4:+8448937	Msa0148200:exon
ACAATCTTTGAAGTTGTTAC+AGG	0.306235	1_4:+8452223	Msa0148200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTCAACAACAAAGAGAA+GGG	+	chr1_4:8448842-8448861	Msa0148200:five_prime_UTR	30.0%
TGAAGTGAAACTGAAAGAAA+CGG	-	chr1_4:8448915-8448934	None:intergenic	30.0%
AGAAAAAACAGTTACAGAAG+TGG	+	chr1_4:8449274-8449293	Msa0148200:intron	30.0%
GAAAAAACAGTTACAGAAGT+GGG	+	chr1_4:8449275-8449294	Msa0148200:intron	30.0%
TCAAACACGTGATAAAAACA+AGG	-	chr1_4:8449360-8449379	None:intergenic	30.0%
TCAATTCTAAGTCAAAGTTG+TGG	+	chr1_4:8449388-8449407	Msa0148200:intron	30.0%
TGTTGCATGTGTAACTATAA+GGG	+	chr1_4:8449486-8449505	Msa0148200:intron	30.0%
GTGTAACTATAAGGGAAAAT+TGG	+	chr1_4:8449494-8449513	Msa0148200:intron	30.0%
GCTTGTACTATTGTTCATTT+AGG	+	chr1_4:8449550-8449569	Msa0148200:intron	30.0%
TGCATATGAATTACATTTGG+TGG	+	chr1_4:8449822-8449841	Msa0148200:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	8448780	8453419	8448780	ID=Msa0148200;Name=Msa0148200
chr1_4	mRNA	8448780	8453419	8448780	ID=Msa0148200-mRNA-1;Parent=Msa0148200;Name=Msa0148200-mRNA-1;_AED=0.20;_eAED=0.20;_QI=166\|1\|1\|1\|1\|1\|5\|311\|264
chr1_4	exon	8448780	8449045	8448780	ID=Msa0148200-mRNA-1:exon:3620;Parent=Msa0148200-mRNA-1
chr1_4	exon	8449148	8449180	8449148	ID=Msa0148200-mRNA-1:exon:3621;Parent=Msa0148200-mRNA-1
chr1_4	exon	8451076	8451304	8451076	ID=Msa0148200-mRNA-1:exon:3622;Parent=Msa0148200-mRNA-1
chr1_4	exon	8452189	8452321	8452189	ID=Msa0148200-mRNA-1:exon:3623;Parent=Msa0148200-mRNA-1
chr1_4	exon	8452809	8453419	8452809	ID=Msa0148200-mRNA-1:exon:3624;Parent=Msa0148200-mRNA-1
chr1_4	five_prime_UTR	8448780	8448945	8448780	ID=Msa0148200-mRNA-1:five_prime_utr;Parent=Msa0148200-mRNA-1
chr1_4	CDS	8448946	8449045	8448946	ID=Msa0148200-mRNA-1:cds;Parent=Msa0148200-mRNA-1
chr1_4	CDS	8449148	8449180	8449148	ID=Msa0148200-mRNA-1:cds;Parent=Msa0148200-mRNA-1
chr1_4	CDS	8451076	8451304	8451076	ID=Msa0148200-mRNA-1:cds;Parent=Msa0148200-mRNA-1
chr1_4	CDS	8452189	8452321	8452189	ID=Msa0148200-mRNA-1:cds;Parent=Msa0148200-mRNA-1
chr1_4	CDS	8452809	8453108	8452809	ID=Msa0148200-mRNA-1:cds;Parent=Msa0148200-mRNA-1
chr1_4	three_prime_UTR	8453109	8453419	8453109	ID=Msa0148200-mRNA-1:three_prime_utr;Parent=Msa0148200-mRNA-1

Gene Sequence

>Msa0148200

ATGGACGTTGTAGGTGCTCCGTACAACCCTCGTACTGTCGAAGAAGTCTTTCGAGATTTCAAAGGTCGCAGAGCTGCTTTAATTAAAGCCCTCACCACCGATGTCGATGTATTCTACCAGCAGTGTGATCCCGAGAAGGAAAATCTTTGCCTTTATGGATTTCCGAGCGAGCAGTGGGAGGTGAATCTGCCAGCTGAGGAAGTGCCGCCTGAGCTTCCAGAGCCAGCATTGGGTATTAACTTTGCTAGAGATGGAATGCAAGAAAAGGATTGGCTATCTTTGGTTGCAGTACACAGTGATGCTTGGTTGCTTTCTGTGGCTTTTTATTTCGGTGCTAGGTTTGGATTTGACAAGGCTGATAGGAAGCGACTGTTTAATATGATCAATGATCTTCCAACAATCTTTGAAGTTGTTACAGGGTCTGGCAGGAAACAAGGAAAGGAAAAGTCATCAGTTTCTAATAATAGCAGTACCAAATCCAAATCAAACTCTAAGCGAAGTTCTGAACCTCAGCCAAAGTTTTCAAAACCAATGGCAATGGCAATGCAGATGCAGTCAAAGGATGAGGTTGAGGATGAAGATGGTGTAGGTGTGGACGAGGAAGATGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGACGAGTTCTGGATTTGCTGCGACATATGTGAGAAATGGTTTCACGGAAAATGCGTGAAGATTACTCCGGCAAGGGCTGAGCATATCAAACAGTACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA

Protein sequence

>Msa0148200

MDVVGAPYNPRTVEEVFRDFKGRRAALIKALTTDVDVFYQQCDPEKENLCLYGFPSEQWEVNLPAEEVPPELPEPALGINFARDGMQEKDWLSLVAVHSDAWLLSVAFYFGARFGFDKADRKRLFNMINDLPTIFEVVTGSGRKQGKEKSSVSNNSSTKSKSNSKRSSEPQPKFSKPMAMAMQMQSKDEVEDEDGVGVDEEDEEEHGETLCGACGDSYAADEFWICCDICEKWFHGKCVKITPARAEHIKQYKCPACSNKRARP*