AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0033430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033430 MtrunA17_Chr1g0182381 95.720 257 11 0 1 257 1 257 1.26e-175 483
Msa0033430 MtrunA17_Chr7g0263011 44.706 255 124 5 1 243 1 250 5.85e-37 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033430 AT3G53670.1 41.007 139 76 4 78 215 66 199 2.90e-15 72.8
Msa0033430 AT3G53670.2 41.007 139 76 4 78 215 66 199 2.90e-15 72.8
Msa0033430 AT2G37480.1 42.520 127 54 4 73 193 58 171 2.83e-14 69.7
Msa0033430 AT2G37480.2 41.732 127 54 4 73 193 58 170 2.20e-13 67.4

Find 90 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTACAAACGTGTCGTTTA+AGG 0.134390 1_1:+60933180 Msa0033430:five_prime_UTR
AAGCGATTTCAGATCGAAAT+TGG 0.164469 1_1:-60932832 None:intergenic
AAATGAAATTGTCTGTTAAA+TGG 0.168633 1_1:+60935042 Msa0033430:CDS
TTATTTATGCAGTCTTGATA+TGG 0.236888 1_1:-60935482 None:intergenic
TGATGATAAGAAAGCGAAAT+TGG 0.267644 1_1:-60932618 None:intergenic
AATCCTTTGCCACAAGATTC+TGG 0.274308 1_1:-60935239 None:intergenic
GGTAGTAGTAACTGTAATAT+TGG 0.282358 1_1:+60934069 Msa0033430:CDS
AGTAAACTTGAAATTACTTT+TGG 0.301816 1_1:+60933985 Msa0033430:CDS
CTTGAAGGCAACACCGTATC+AGG 0.316264 1_1:-60934177 None:intergenic
GTAGTAGTAACTGTAATATT+GGG 0.335267 1_1:+60934070 Msa0033430:CDS
TTTCTCGCGGCTACCCTTCA+TGG 0.337552 1_1:-60935006 None:intergenic
TCCGACATGGCAAGGACTTT+CGG 0.354930 1_1:-60935345 None:intergenic
TTAAATAATCTCGACGTTGC+TGG 0.364240 1_1:+60935278 Msa0033430:CDS
TGCAATTTGATTCCGTTTCA+CGG 0.366326 1_1:+60932910 Msa0033430:five_prime_UTR
CACTCTCCTGTGAATGTTGT+CGG 0.383816 1_1:+60933949 Msa0033430:CDS
TATTTATGCAGTCTTGATAT+GGG 0.394789 1_1:-60935481 None:intergenic
AGATCGAAGAACATGCGAAA+TGG 0.409219 1_1:-60932779 None:intergenic
AATGAAATTGTCTGTTAAAT+GGG 0.413068 1_1:+60935043 Msa0033430:CDS
CAAAGGATTTGATGCTTCAA+TGG 0.415687 1_1:+60935253 Msa0033430:CDS
CGAAATGGATCTGAGAGTTA+TGG 0.419952 1_1:-60932764 None:intergenic
TTGATTCACGGTTGAATTTG+AGG 0.426338 1_1:+60933078 Msa0033430:five_prime_UTR
ATTCCAAGAGATACATTGAT+TGG 0.426598 1_1:-60935571 None:intergenic
AACAAAACAAAACTCTCATA+TGG 0.430420 1_1:+60932663 Msa0033430:five_prime_UTR
AACCTTTCCTTATCCTGATA+CGG 0.431985 1_1:+60934164 Msa0033430:CDS
AATTGCGAAAATCTCAAAAC+AGG 0.441311 1_1:-60933154 None:intergenic
TAAATAATTAGTTTCCTGTT+CGG 0.446370 1_1:+60935498 Msa0033430:three_prime_UTR
TCGTCATCAGAAGAGCTTGA+AGG 0.452918 1_1:-60934192 None:intergenic
TGGGAAATCCTTTGTTCGAT+CGG 0.453138 1_1:-60932812 None:intergenic
GACTTATGCAGTCTTGATAT+AGG 0.453851 1_1:-60935440 None:intergenic
AGCGATTTCAGATCGAAATT+GGG 0.457477 1_1:-60932831 None:intergenic
TCTTCTTCACACAACCGAAC+AGG 0.460962 1_1:-60935512 None:intergenic
CATATGTGGTGGGTTTGAAC+AGG 0.469286 1_1:+60934101 Msa0033430:CDS
TCCAGACTCGGTTTCTCAGC+CGG 0.470198 1_1:+60934257 Msa0033430:CDS
ACCGGCTGAGAAACCGAGTC+TGG 0.472530 1_1:-60934258 None:intergenic
GACTTATGCAGTCTTGATAT+AGG 0.475805 1_1:-60935399 None:intergenic
TGGCAAGGACTTTCGGTAGC+TGG 0.476961 1_1:-60935338 None:intergenic
AGAAGCAGTATCGCTATAGA+TGG 0.477970 1_1:+60935216 Msa0033430:CDS
GTGTGATCTTATACGATTGA+TGG 0.483125 1_1:+60933921 Msa0033430:intron
TCTGATGACGAGGAGGCTGA+TGG 0.485008 1_1:+60934204 Msa0033430:CDS
CTCTACACAACTTCCAGACT+CGG 0.490684 1_1:+60934245 Msa0033430:CDS
GGCAACACCGTATCAGGATA+AGG 0.496540 1_1:-60934171 None:intergenic
CATGTTCTTCGATCTTCGAT+CGG 0.503699 1_1:+60932787 Msa0033430:five_prime_UTR
GGCACCGGATCATACACATC+AGG 0.503927 1_1:-60935068 None:intergenic
CAGAAGGGCAGCTATTCTAA+AGG 0.505063 1_1:+60935176 Msa0033430:CDS
TGGCCAATCAATGTATCTCT+TGG 0.506192 1_1:+60935568 Msa0033430:three_prime_UTR
ATGTTGTTTCTTAGTTCTCA+CGG 0.517557 1_1:-60935108 None:intergenic
AAAGAAGATCCACCGTGAAA+CGG 0.518001 1_1:-60932922 None:intergenic
ACTCTCCTGTGAATGTTGTC+GGG 0.518111 1_1:+60933950 Msa0033430:CDS
TTGACTTCAAAGATTGTGAA+AGG 0.525582 1_1:+60933115 Msa0033430:five_prime_UTR
ACTAAAAGTGAGGAGCTTGA+AGG 0.525677 1_1:+60934134 Msa0033430:CDS
AGAAGGGCAGCTATTCTAAA+GGG 0.525902 1_1:+60935177 Msa0033430:CDS
AAAACTTGTATCTGCCATGA+AGG 0.537609 1_1:+60934992 Msa0033430:CDS
TGCTCAAAGCTTCTCCGACA+TGG 0.545181 1_1:-60935358 None:intergenic
GATCGGTTCCGATCGAACAA+AGG 0.548374 1_1:+60932804 Msa0033430:five_prime_UTR
GAATTAGAATCGAATCGAAT+CGG 0.550153 1_1:-60932733 None:intergenic
GGGTAGCCGCGAGAAACACT+TGG 0.556191 1_1:+60935013 Msa0033430:CDS
TGGCCAGAATCTTGTGGCAA+AGG 0.557338 1_1:+60935236 Msa0033430:CDS
AAAGCTTCTCCGACATGGCA+AGG 0.559480 1_1:-60935353 None:intergenic
AGGTGTAAAGAAAAGTCAGA+AGG 0.565051 1_1:+60935160 Msa0033430:CDS
TCGCACCCGACAACATTCAC+AGG 0.571872 1_1:-60933955 None:intergenic
CTAAGAAACAACATAAATCC+CGG 0.580812 1_1:+60935117 Msa0033430:CDS
ATTGGGGAGGGACACATATG+TGG 0.592835 1_1:+60934087 Msa0033430:CDS
AGTAACTGTAATATTGGGGA+GGG 0.594128 1_1:+60934075 Msa0033430:CDS
CGACAACATTCACAGGAGAG+TGG 0.598576 1_1:-60933948 None:intergenic
GGCCCCTGATGTGTATGATC+CGG 0.600130 1_1:+60935064 Msa0033430:CDS
ATCAAGAAGAGTGAAAAGAA+AGG 0.600790 1_1:+60935140 Msa0033430:CDS
TGTGACGACAATGTTGGCAC+CGG 0.602355 1_1:-60935083 None:intergenic
TAGTAGTAACTGTAATATTG+GGG 0.605374 1_1:+60934071 Msa0033430:CDS
TGATATAGGAGACAAAATCA+AGG 0.608502 1_1:-60935385 None:intergenic
TTCAAGCTCTTCTGATGACG+AGG 0.614405 1_1:+60934194 Msa0033430:CDS
GGTGTAAAGAAAAGTCAGAA+GGG 0.615586 1_1:+60935161 Msa0033430:CDS
GGGGAGGGACACATATGTGG+TGG 0.617375 1_1:+60934090 Msa0033430:CDS
ACGGTATGTGACGACAATGT+TGG 0.618744 1_1:-60935089 None:intergenic
GGGAGGGACACATATGTGGT+GGG 0.620596 1_1:+60934091 Msa0033430:CDS
GAAACGGAATCAAATTGCAA+AGG 0.620899 1_1:-60932906 None:intergenic
AAACTTGTATCTGCCATGAA+GGG 0.622758 1_1:+60934993 Msa0033430:CDS
GCACCGGATCATACACATCA+GGG 0.623292 1_1:-60935067 None:intergenic
ACCGAAAGTCCTTGCCATGT+CGG 0.626598 1_1:+60935344 Msa0033430:CDS
CACCGTATCAGGATAAGGAA+AGG 0.628831 1_1:-60934166 None:intergenic
TATAGATGGCCAGAATCTTG+TGG 0.629442 1_1:+60935230 Msa0033430:CDS
GGTAGCCGCGAGAAACACTT+GGG 0.637014 1_1:+60935014 Msa0033430:CDS
TAGTAACTGTAATATTGGGG+AGG 0.638150 1_1:+60934074 Msa0033430:CDS
ACATGTATCTGATGATGATG+AGG 0.654147 1_1:+60934044 Msa0033430:CDS
AAGCTCTTCTGATGACGAGG+AGG 0.662047 1_1:+60934197 Msa0033430:CDS
AAGAAACCACACTAAAAGTG+AGG 0.668513 1_1:+60934124 Msa0033430:CDS
GCGATCCCAAGTGTTTCTCG+CGG 0.669443 1_1:-60935019 None:intergenic
CACCGGATCATACACATCAG+GGG 0.699399 1_1:-60935066 None:intergenic
GTATCTGATGATGATGAGGA+TGG 0.713196 1_1:+60934048 Msa0033430:CDS
TTTCTGTGTTAAATAATGTG+TGG 0.717143 1_1:+60935630 Msa0033430:three_prime_UTR
AATTTGATTCCGTTTCACGG+TGG 0.736766 1_1:+60932913 Msa0033430:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAGATGAAAAAACTT+TGG - chr1_1:60933030-60933049 None:intergenic 15.0%
!!! AAGTTTTTTCATCTATTTTT+TGG + chr1_1:60933030-60933049 Msa0033430:five_prime_UTR 15.0%
!! AATACAAAATTGAAAATTCA+TGG - chr1_1:60933287-60933306 None:intergenic 15.0%
!!! AACATACACTTTATTTTAAA+TGG + chr1_1:60934763-60934782 Msa0033430:intron 15.0%
!!! ACATACACTTTATTTTAAAT+GGG + chr1_1:60934764-60934783 Msa0033430:intron 15.0%
!!! ATATTTGCTGACAATTTATA+TGG + chr1_1:60933441-60933460 Msa0033430:intron 20.0%
!! GCTAATTAATGAATATAACA+AGG + chr1_1:60933463-60933482 Msa0033430:intron 20.0%
!! TCAACAAATTAGATAGTTAA+CGG - chr1_1:60933624-60933643 None:intergenic 20.0%
!! TTTACAATATTAGTGTATAG+TGG + chr1_1:60933702-60933721 Msa0033430:intron 20.0%
!!! AGTAAACTTGAAATTACTTT+TGG + chr1_1:60933985-60934004 Msa0033430:CDS 20.0%
!! TAAGAAATGTAGTTTATGTT+TGG + chr1_1:60934332-60934351 Msa0033430:intron 20.0%
!!! ACTTTATTTTAAATGGGAAA+GGG + chr1_1:60934770-60934789 Msa0033430:intron 20.0%
!! AAATGAAATTGTCTGTTAAA+TGG + chr1_1:60935042-60935061 Msa0033430:CDS 20.0%
!! AATGAAATTGTCTGTTAAAT+GGG + chr1_1:60935043-60935062 Msa0033430:CDS 20.0%
!!! TAAATAATTAGTTTCCTGTT+CGG + chr1_1:60935498-60935517 Msa0033430:three_prime_UTR 20.0%
! AACAAAACAAAACTCTCATA+TGG + chr1_1:60932663-60932682 Msa0033430:five_prime_UTR 25.0%
!!! TGTGTTTTTTCTGTTAATTG+AGG - chr1_1:60932699-60932718 None:intergenic 25.0%
!!! AAATCGCTTTTGTAACAATT+AGG + chr1_1:60932846-60932865 Msa0033430:five_prime_UTR 25.0%
!!! AATCGCTTTTGTAACAATTA+GGG + chr1_1:60932847-60932866 Msa0033430:five_prime_UTR 25.0%
! AACATCTTGAAAAAAGTGAT+AGG + chr1_1:60932956-60932975 Msa0033430:five_prime_UTR 25.0%
!!! ATTTTTTGGAGTATTTCTTC+TGG + chr1_1:60933044-60933063 Msa0033430:five_prime_UTR 25.0%
!!! GTTTTGTGAATTTTGATTCA+CGG + chr1_1:60933066-60933085 Msa0033430:five_prime_UTR 25.0%
! TATGATTCAACCTAATTTCT+AGG + chr1_1:60933250-60933269 Msa0033430:intron 25.0%
! CTATATTCAACCTAGAAATT+AGG - chr1_1:60933263-60933282 None:intergenic 25.0%
!! CTAATTTCTAGGTTGAATAT+AGG + chr1_1:60933261-60933280 Msa0033430:intron 25.0%
! ATATTAGTGTATAGTGGTAT+TGG + chr1_1:60933708-60933727 Msa0033430:intron 25.0%
!!! AAACTTGAAATTACTTTTGG+TGG + chr1_1:60933988-60934007 Msa0033430:CDS 25.0%
!!! AACTTGAAATTACTTTTGGT+GGG + chr1_1:60933989-60934008 Msa0033430:CDS 25.0%
! GTAGTAGTAACTGTAATATT+GGG + chr1_1:60934070-60934089 Msa0033430:CDS 25.0%
! TAGTAGTAACTGTAATATTG+GGG + chr1_1:60934071-60934090 Msa0033430:CDS 25.0%
! AAATGTAGTTTATGTTTGGA+CGG + chr1_1:60934336-60934355 Msa0033430:intron 25.0%
! AAGTGTATGTTTGTATCATA+TGG - chr1_1:60934516-60934535 None:intergenic 25.0%
!!! ACATACACTTTATTTTGAAG+TGG + chr1_1:60934526-60934545 Msa0033430:intron 25.0%
!! TATGTTTTGTATCATATGGA+GGG - chr1_1:60934749-60934768 None:intergenic 25.0%
!! AGTGTATGTTTTGTATCATA+TGG - chr1_1:60934753-60934772 None:intergenic 25.0%
!!! CACTTTATTTTAAATGGGAA+AGG + chr1_1:60934769-60934788 Msa0033430:intron 25.0%
! TATTTATGCAGTCTTGATAT+GGG - chr1_1:60935484-60935503 None:intergenic 25.0%
! TTATTTATGCAGTCTTGATA+TGG - chr1_1:60935485-60935504 None:intergenic 25.0%
!!! GAAGAAGAGTTTTTTGTTTA+CGG + chr1_1:60935527-60935546 Msa0033430:three_prime_UTR 25.0%
! TTTCTGTGTTAAATAATGTG+TGG + chr1_1:60935630-60935649 Msa0033430:three_prime_UTR 25.0%
TGATGATAAGAAAGCGAAAT+TGG - chr1_1:60932621-60932640 None:intergenic 30.0%
GAATTAGAATCGAATCGAAT+CGG - chr1_1:60932736-60932755 None:intergenic 30.0%
! AACGAAACTAAAAAAGCGTA+AGG - chr1_1:60933000-60933019 None:intergenic 30.0%
TTGACTTCAAAGATTGTGAA+AGG + chr1_1:60933115-60933134 Msa0033430:five_prime_UTR 30.0%
AATTGCGAAAATCTCAAAAC+AGG - chr1_1:60933157-60933176 None:intergenic 30.0%
CATGATTTATCAGCTATGAA+TGG + chr1_1:60933389-60933408 Msa0033430:intron 30.0%
GGTTAATTGTGTTCAATTGT+TGG - chr1_1:60933505-60933524 None:intergenic 30.0%
!!! TGTTTTGTCGATTTGTTCTT+TGG + chr1_1:60933892-60933911 Msa0033430:intron 30.0%
!!! GTTTTGTCGATTTGTTCTTT+GGG + chr1_1:60933893-60933912 Msa0033430:intron 30.0%
GGTAGTAGTAACTGTAATAT+TGG + chr1_1:60934069-60934088 Msa0033430:CDS 30.0%
TGAACACAATTAGAACTTAC+CGG - chr1_1:60934279-60934298 None:intergenic 30.0%
GTATGTTTGTATCATATGGT+GGG - chr1_1:60934512-60934531 None:intergenic 30.0%
TGTATGTTTGTATCATATGG+TGG - chr1_1:60934513-60934532 None:intergenic 30.0%
!! GAAAGAACTTTTGACTCATT+CGG + chr1_1:60934548-60934567 Msa0033430:intron 30.0%
! TTATGCTAGATGCATTGTTT+TGG + chr1_1:60934654-60934673 Msa0033430:intron 30.0%
! GTATGTTTTGTATCATATGG+AGG - chr1_1:60934750-60934769 None:intergenic 30.0%
!!! ATAGTTTTGTCGAGCATAAT+TGG + chr1_1:60934903-60934922 Msa0033430:intron 30.0%
! TTTTCTCTGTATTATACCAC+AGG + chr1_1:60934962-60934981 Msa0033430:intron 30.0%
!! ATGTTGTTTCTTAGTTCTCA+CGG - chr1_1:60935111-60935130 None:intergenic 30.0%
CTAAGAAACAACATAAATCC+CGG + chr1_1:60935117-60935136 Msa0033430:CDS 30.0%
ATCAAGAAGAGTGAAAAGAA+AGG + chr1_1:60935140-60935159 Msa0033430:CDS 30.0%
TGATATAGGAGACAAAATCA+AGG - chr1_1:60935388-60935407 None:intergenic 30.0%
ATTCCAAGAGATACATTGAT+TGG - chr1_1:60935574-60935593 None:intergenic 30.0%
! TGTGTGGTAGCTTTTATATT+TGG + chr1_1:60935646-60935665 Msa0033430:three_prime_UTR 30.0%
AGCGATTTCAGATCGAAATT+GGG - chr1_1:60932834-60932853 None:intergenic 35.0%
AAGCGATTTCAGATCGAAAT+TGG - chr1_1:60932835-60932854 None:intergenic 35.0%
GAAACGGAATCAAATTGCAA+AGG - chr1_1:60932909-60932928 None:intergenic 35.0%
TGCAATTTGATTCCGTTTCA+CGG + chr1_1:60932910-60932929 Msa0033430:five_prime_UTR 35.0%
TTGATTCACGGTTGAATTTG+AGG + chr1_1:60933078-60933097 Msa0033430:five_prime_UTR 35.0%
!! ATCTACAAACGTGTCGTTTA+AGG + chr1_1:60933180-60933199 Msa0033430:five_prime_UTR 35.0%
! GTTCTGAACAATAAGACACT+AGG - chr1_1:60933320-60933339 None:intergenic 35.0%
ATGGTAAGTGATGTTCCAAA+GGG - chr1_1:60933529-60933548 None:intergenic 35.0%
TTGTGAACACTTGCAAATCA+TGG - chr1_1:60933548-60933567 None:intergenic 35.0%
ACGGTTTGACACATTTAAGT+TGG - chr1_1:60933605-60933624 None:intergenic 35.0%
GAATAGTAGACAATGTTGTC+TGG + chr1_1:60933674-60933693 Msa0033430:intron 35.0%
GGAAGTAATCAACAAGAAGA+AGG - chr1_1:60933776-60933795 None:intergenic 35.0%
AAGTAAAACAGTCGACACAT+AGG - chr1_1:60933818-60933837 None:intergenic 35.0%
GTGTGATCTTATACGATTGA+TGG + chr1_1:60933921-60933940 Msa0033430:intron 35.0%
! ACATGTATCTGATGATGATG+AGG + chr1_1:60934044-60934063 Msa0033430:CDS 35.0%
TAGTAACTGTAATATTGGGG+AGG + chr1_1:60934074-60934093 Msa0033430:CDS 35.0%
AGTAACTGTAATATTGGGGA+GGG + chr1_1:60934075-60934094 Msa0033430:CDS 35.0%
AAGAAACCACACTAAAAGTG+AGG + chr1_1:60934124-60934143 Msa0033430:CDS 35.0%
AACCTTTCCTTATCCTGATA+CGG + chr1_1:60934164-60934183 Msa0033430:CDS 35.0%
! CTATAGCTTTTGCAAAACCA+CGG - chr1_1:60934388-60934407 None:intergenic 35.0%
GAAATTACTGTGAACAACTG+TGG + chr1_1:60934415-60934434 Msa0033430:intron 35.0%
TTGTAGCTTTCACACAATCA+CGG + chr1_1:60934463-60934482 Msa0033430:intron 35.0%
!!! TTGTTTTGGTCGAGCATAAT+TGG + chr1_1:60934668-60934687 Msa0033430:intron 35.0%
ATGCAGCTTTCACATAATCA+CGG + chr1_1:60934701-60934720 Msa0033430:intron 35.0%
CTCATTCAACAAATAGAAGC+TGG + chr1_1:60934799-60934818 Msa0033430:intron 35.0%
AAAACTTGTATCTGCCATGA+AGG + chr1_1:60934992-60935011 Msa0033430:CDS 35.0%
AAACTTGTATCTGCCATGAA+GGG + chr1_1:60934993-60935012 Msa0033430:CDS 35.0%
! TCTTTTCACTCTTCTTGATC+CGG - chr1_1:60935139-60935158 None:intergenic 35.0%
AGGTGTAAAGAAAAGTCAGA+AGG + chr1_1:60935160-60935179 Msa0033430:CDS 35.0%
GGTGTAAAGAAAAGTCAGAA+GGG + chr1_1:60935161-60935180 Msa0033430:CDS 35.0%
CAAAGGATTTGATGCTTCAA+TGG + chr1_1:60935253-60935272 Msa0033430:CDS 35.0%
TTAAATAATCTCGACGTTGC+TGG + chr1_1:60935278-60935297 Msa0033430:CDS 35.0%
!!! TTTTTGAGATGCTAGTTGCA+TGG - chr1_1:60935316-60935335 None:intergenic 35.0%
GACTTATGCAGTCTTGATAT+AGG - chr1_1:60935402-60935421 None:intergenic 35.0%
GACTTATGCAGTCTTGATAT+AGG - chr1_1:60935402-60935421 None:intergenic 35.0%
GGTTAAAAACTATTCAGTGC+TGG + chr1_1:60935548-60935567 Msa0033430:three_prime_UTR 35.0%
CGAAATGGATCTGAGAGTTA+TGG - chr1_1:60932767-60932786 None:intergenic 40.0%
AGATCGAAGAACATGCGAAA+TGG - chr1_1:60932782-60932801 None:intergenic 40.0%
CATGTTCTTCGATCTTCGAT+CGG + chr1_1:60932787-60932806 Msa0033430:five_prime_UTR 40.0%
TGGGAAATCCTTTGTTCGAT+CGG - chr1_1:60932815-60932834 None:intergenic 40.0%
AATTTGATTCCGTTTCACGG+TGG + chr1_1:60932913-60932932 Msa0033430:five_prime_UTR 40.0%
AAAGAAGATCCACCGTGAAA+CGG - chr1_1:60932925-60932944 None:intergenic 40.0%
CTTGAAAAAAGTGATAGGCG+TGG + chr1_1:60932961-60932980 Msa0033430:five_prime_UTR 40.0%
GAACACAATTAACCACCCTT+TGG + chr1_1:60933511-60933530 Msa0033430:intron 40.0%
CATGGTAAGTGATGTTCCAA+AGG - chr1_1:60933530-60933549 None:intergenic 40.0%
! GTATCTGATGATGATGAGGA+TGG + chr1_1:60934048-60934067 Msa0033430:CDS 40.0%
ACTAAAAGTGAGGAGCTTGA+AGG + chr1_1:60934134-60934153 Msa0033430:CDS 40.0%
TGTAGTTTATGTTTGGACGG+TGG + chr1_1:60934339-60934358 Msa0033430:intron 40.0%
GTAGTTTATGTTTGGACGGT+GGG + chr1_1:60934340-60934359 Msa0033430:intron 40.0%
TGGGTTGCCGAAATCATTAT+GGG + chr1_1:60934359-60934378 Msa0033430:intron 40.0%
ACGGTTGCCCATAATGATTT+CGG - chr1_1:60934369-60934388 None:intergenic 40.0%
! ATGGCAGATACAAGTTTTGC+AGG - chr1_1:60934990-60935009 None:intergenic 40.0%
! CTTTTCACTCTTCTTGATCC+GGG - chr1_1:60935138-60935157 None:intergenic 40.0%
AGAAGGGCAGCTATTCTAAA+GGG + chr1_1:60935177-60935196 Msa0033430:CDS 40.0%
AGAAGCAGTATCGCTATAGA+TGG + chr1_1:60935216-60935235 Msa0033430:CDS 40.0%
TATAGATGGCCAGAATCTTG+TGG + chr1_1:60935230-60935249 Msa0033430:CDS 40.0%
AATCCTTTGCCACAAGATTC+TGG - chr1_1:60935242-60935261 None:intergenic 40.0%
TGGCCAATCAATGTATCTCT+TGG + chr1_1:60935568-60935587 Msa0033430:three_prime_UTR 40.0%
GTAAGTGATGTTCCAAAGGG+TGG - chr1_1:60933526-60933545 None:intergenic 45.0%
CACTCTCCTGTGAATGTTGT+CGG + chr1_1:60933949-60933968 Msa0033430:CDS 45.0%
ACTCTCCTGTGAATGTTGTC+GGG + chr1_1:60933950-60933969 Msa0033430:CDS 45.0%
!! CATATGTGGTGGGTTTGAAC+AGG + chr1_1:60934101-60934120 Msa0033430:CDS 45.0%
!! CAAGCTCCTCACTTTTAGTG+TGG - chr1_1:60934133-60934152 None:intergenic 45.0%
CACCGTATCAGGATAAGGAA+AGG - chr1_1:60934169-60934188 None:intergenic 45.0%
TCGTCATCAGAAGAGCTTGA+AGG - chr1_1:60934195-60934214 None:intergenic 45.0%
! TTCAAGCTCTTCTGATGACG+AGG + chr1_1:60934194-60934213 Msa0033430:CDS 45.0%
CTCTACACAACTTCCAGACT+CGG + chr1_1:60934245-60934264 Msa0033430:CDS 45.0%
! GTGGGTTGCCGAAATCATTA+TGG + chr1_1:60934358-60934377 Msa0033430:intron 45.0%
GAAATCATTATGGGCAACCG+TGG + chr1_1:60934368-60934387 Msa0033430:intron 45.0%
ACGGTTGCTAGCCATGATTT+CGG + chr1_1:60934482-60934501 Msa0033430:intron 45.0%
TCATTCGGCAGATAGAAAGC+TGG + chr1_1:60934563-60934582 Msa0033430:intron 45.0%
AACTACTTTCCACCCCCATT+GGG - chr1_1:60934829-60934848 None:intergenic 45.0%
ACGGTATGTGACGACAATGT+TGG - chr1_1:60935092-60935111 None:intergenic 45.0%
CAGAAGGGCAGCTATTCTAA+AGG + chr1_1:60935176-60935195 Msa0033430:CDS 45.0%
TCTTCTTCACACAACCGAAC+AGG - chr1_1:60935515-60935534 None:intergenic 45.0%
! GATCGGTTCCGATCGAACAA+AGG + chr1_1:60932804-60932823 Msa0033430:five_prime_UTR 50.0%
GGTACGCGAGCTCTATGATT+AGG - chr1_1:60933797-60933816 None:intergenic 50.0%
CGACAACATTCACAGGAGAG+TGG - chr1_1:60933951-60933970 None:intergenic 50.0%
ATTGGGGAGGGACACATATG+TGG + chr1_1:60934087-60934106 Msa0033430:CDS 50.0%
GGCAACACCGTATCAGGATA+AGG - chr1_1:60934174-60934193 None:intergenic 50.0%
CTTGAAGGCAACACCGTATC+AGG - chr1_1:60934180-60934199 None:intergenic 50.0%
! AAGCTCTTCTGATGACGAGG+AGG + chr1_1:60934197-60934216 Msa0033430:CDS 50.0%
!! TGGTGGGTTAGCCGAAATCA+TGG - chr1_1:60934496-60934515 None:intergenic 50.0%
ATAGAAAGCTGGTCGCTCCA+AGG + chr1_1:60934574-60934593 Msa0033430:intron 50.0%
TAGAAAGCTGGTCGCTCCAA+GGG + chr1_1:60934575-60934594 Msa0033430:intron 50.0%
ATAGAAGCTGGTCACCCCAA+TGG + chr1_1:60934811-60934830 Msa0033430:intron 50.0%
TAGAAGCTGGTCACCCCAAT+GGG + chr1_1:60934812-60934831 Msa0033430:intron 50.0%
ACTACTTTCCACCCCCATTG+GGG - chr1_1:60934828-60934847 None:intergenic 50.0%
CAACTACTTTCCACCCCCAT+TGG - chr1_1:60934830-60934849 None:intergenic 50.0%
CAATGGGGGTGGAAAGTAGT+TGG + chr1_1:60934828-60934847 Msa0033430:intron 50.0%
! ACAAGTTTTGCAGGTGCCTG+TGG - chr1_1:60934981-60935000 None:intergenic 50.0%
CACCGGATCATACACATCAG+GGG - chr1_1:60935069-60935088 None:intergenic 50.0%
GCACCGGATCATACACATCA+GGG - chr1_1:60935070-60935089 None:intergenic 50.0%
TGTGACGACAATGTTGGCAC+CGG - chr1_1:60935086-60935105 None:intergenic 50.0%
TGGCCAGAATCTTGTGGCAA+AGG + chr1_1:60935236-60935255 Msa0033430:CDS 50.0%
ACCGAAAGTCCTTGCCATGT+CGG + chr1_1:60935344-60935363 Msa0033430:CDS 50.0%
TCCGACATGGCAAGGACTTT+CGG - chr1_1:60935348-60935367 None:intergenic 50.0%
AAAGCTTCTCCGACATGGCA+AGG - chr1_1:60935356-60935375 None:intergenic 50.0%
TGCTCAAAGCTTCTCCGACA+TGG - chr1_1:60935361-60935380 None:intergenic 50.0%
TCGCACCCGACAACATTCAC+AGG - chr1_1:60933958-60933977 None:intergenic 55.0%
GGGAGGGACACATATGTGGT+GGG + chr1_1:60934091-60934110 Msa0033430:CDS 55.0%
! TCTGATGACGAGGAGGCTGA+TGG + chr1_1:60934204-60934223 Msa0033430:CDS 55.0%
! TCCAGACTCGGTTTCTCAGC+CGG + chr1_1:60934257-60934276 Msa0033430:CDS 55.0%
AGAAAGCTGGTCGCTCCAAG+GGG + chr1_1:60934576-60934595 Msa0033430:intron 55.0%
CAACTACTTTCCACCCCCCT+TGG - chr1_1:60934594-60934613 None:intergenic 55.0%
CAAGGGGGGTGGAAAGTAGT+TGG + chr1_1:60934592-60934611 Msa0033430:intron 55.0%
AGAAGCTGGTCACCCCAATG+GGG + chr1_1:60934813-60934832 Msa0033430:intron 55.0%
TTTCTCGCGGCTACCCTTCA+TGG - chr1_1:60935009-60935028 None:intergenic 55.0%
GGTAGCCGCGAGAAACACTT+GGG + chr1_1:60935014-60935033 Msa0033430:CDS 55.0%
GCGATCCCAAGTGTTTCTCG+CGG - chr1_1:60935022-60935041 None:intergenic 55.0%
GGCCCCTGATGTGTATGATC+CGG + chr1_1:60935064-60935083 Msa0033430:CDS 55.0%
! GGCACCGGATCATACACATC+AGG - chr1_1:60935071-60935090 None:intergenic 55.0%
TGGCAAGGACTTTCGGTAGC+TGG - chr1_1:60935341-60935360 None:intergenic 55.0%
GGGGAGGGACACATATGTGG+TGG + chr1_1:60934090-60934109 Msa0033430:CDS 60.0%
ACCGGCTGAGAAACCGAGTC+TGG - chr1_1:60934261-60934280 None:intergenic 60.0%
GAAAGCTGGTCGCTCCAAGG+GGG + chr1_1:60934577-60934596 Msa0033430:intron 60.0%
AAAGCTGGTCGCTCCAAGGG+GGG + chr1_1:60934578-60934597 Msa0033430:intron 60.0%
GAAGCTGGTCACCCCAATGG+GGG + chr1_1:60934814-60934833 Msa0033430:intron 60.0%
GGGTAGCCGCGAGAAACACT+TGG + chr1_1:60935013-60935032 Msa0033430:CDS 60.0%
GCTGGTCACCCCAATGGGGG+TGG + chr1_1:60934817-60934836 Msa0033430:intron 70.0%
GCTGGTCGCTCCAAGGGGGG+TGG + chr1_1:60934581-60934600 Msa0033430:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60932591 60935674 60932591 ID=Msa0033430;Name=Msa0033430
chr1_1 mRNA 60932591 60935674 60932591 ID=Msa0033430-mRNA-1;Parent=Msa0033430;Name=Msa0033430-mRNA-1;_AED=0.01;_eAED=0.01;_QI=629|1|1|1|0.5|0.33|3|256|257
chr1_1 exon 60932591 60933201 60932591 ID=Msa0033430-mRNA-1:exon:19088;Parent=Msa0033430-mRNA-1
chr1_1 exon 60933922 60934278 60933922 ID=Msa0033430-mRNA-1:exon:19089;Parent=Msa0033430-mRNA-1
chr1_1 exon 60934984 60935674 60934984 ID=Msa0033430-mRNA-1:exon:19090;Parent=Msa0033430-mRNA-1
chr1_1 five_prime_UTR 60932591 60933201 60932591 ID=Msa0033430-mRNA-1:five_prime_utr;Parent=Msa0033430-mRNA-1
chr1_1 five_prime_UTR 60933922 60933939 60933922 ID=Msa0033430-mRNA-1:five_prime_utr;Parent=Msa0033430-mRNA-1
chr1_1 CDS 60933940 60934278 60933940 ID=Msa0033430-mRNA-1:cds;Parent=Msa0033430-mRNA-1
chr1_1 CDS 60934984 60935418 60934984 ID=Msa0033430-mRNA-1:cds;Parent=Msa0033430-mRNA-1
chr1_1 three_prime_UTR 60935419 60935674 60935419 ID=Msa0033430-mRNA-1:three_prime_utr;Parent=Msa0033430-mRNA-1
Gene Sequence

>Msa0033430

ATGGACATCCACTCTCCTGTGAATGTTGTCGGGTGCGATATCATAAGTAAACTTGAAATTACTTTTGGTGGGTCATTACATATACAAGATGATCAAAAGTTAGAACATGTATCTGATGATGATGAGGATGGTAGTAGTAACTGTAATATTGGGGAGGGACACATATGTGGTGGGTTTGAACAGGAAGAAACCACACTAAAAGTGAGGAGCTTGAAGGAGTGTTCAACCTTTCCTTATCCTGATACGGTGTTGCCTTCAAGCTCTTCTGATGACGAGGAGGCTGATGGAGCATTGACAGAGTCACTCTCTACACAACTTCCAGACTCGGTTTCTCAGCCGGCACCTGCAAAACTTGTATCTGCCATGAAGGGTAGCCGCGAGAAACACTTGGGATCGCAAATGAAATTGTCTGTTAAATGGGCCCCTGATGTGTATGATCCGGTGCCAACATTGTCGTCACATACCGTGAGAACTAAGAAACAACATAAATCCCGGATCAAGAAGAGTGAAAAGAAAGGTGTAAAGAAAAGTCAGAAGGGCAGCTATTCTAAAGGGTGTAGCAGCAAAGATAAGAAGCAGTATCGCTATAGATGGCCAGAATCTTGTGGCAAAGGATTTGATGCTTCAATGGAATTAAATAATCTCGACGTTGCTGGCTATGATTCATACCATGCAACTAGCATCTCAAAAATACCAGCTACCGAAAGTCCTTGCCATGTCGGAGAAGCTTTGAGCAACTTCCTTGATTTTGTCTCCTATATCAAGACTGCATAA
Protein sequence

>Msa0033430

MDIHSPVNVVGCDIISKLEITFGGSLHIQDDQKLEHVSDDDEDGSSNCNIGEGHICGGFEQEETTLKVRSLKECSTFPYPDTVLPSSSSDDEEADGALTESLSTQLPDSVSQPAPAKLVSAMKGSREKHLGSQMKLSVKWAPDVYDPVPTLSSHTVRTKKQHKSRIKKSEKKGVKKSQKGSYSKGCSSKDKKQYRYRWPESCGKGFDASMELNNLDVAGYDSYHATSISKIPATESPCHVGEALSNFLDFVSYIKTA*