Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0039170 | sp|Q67ZM4|ADF7_ARATH | 84.559 | 136 | 21 | 0 | 735 | 870 | 2 | 137 | 5.49e-73 | 238 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0039170 | Msa0045490 | 0.808291 | 3.329722e-50 | -8.615850e-47 |
| Msa0039170 | Msa0133630 | 0.960118 | 3.380531e-118 | -8.615850e-47 |
| Msa0039170 | Msa0864420 | 0.802207 | 6.258492e-49 | -8.615850e-47 |
| Msa0039170 | Msa1376200 | 0.814548 | 1.456866e-51 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0039170 | MtrunA17_Chr1g0203191 | 97.854 | 233 | 5 | 0 | 1 | 233 | 1 | 233 | 1.16e-161 | 471 |
| Msa0039170 | MtrunA17_Chr1g0203201 | 64.626 | 441 | 104 | 8 | 230 | 667 | 1 | 392 | 1.22e-143 | 431 |
| Msa0039170 | MtrunA17_Chr1g0203211 | 97.810 | 137 | 3 | 0 | 734 | 870 | 25 | 161 | 1.55e-90 | 283 |
| Msa0039170 | MtrunA17_Chr5g0397641 | 83.088 | 136 | 23 | 0 | 735 | 870 | 2 | 137 | 8.12e-73 | 235 |
| Msa0039170 | MtrunA17_Chr8g0386651 | 78.676 | 136 | 29 | 0 | 735 | 870 | 2 | 137 | 1.29e-68 | 223 |
| Msa0039170 | MtrunA17_Chr1g0182501 | 74.453 | 137 | 33 | 1 | 735 | 869 | 2 | 138 | 6.40e-68 | 221 |
| Msa0039170 | MtrunA17_Chr2g0291571 | 71.533 | 137 | 37 | 1 | 735 | 869 | 2 | 138 | 1.94e-65 | 215 |
| Msa0039170 | MtrunA17_Chr8g0380291 | 70.073 | 137 | 39 | 1 | 735 | 869 | 2 | 138 | 4.59e-64 | 211 |
| Msa0039170 | MtrunA17_Chr7g0261251 | 60.294 | 136 | 52 | 1 | 736 | 869 | 10 | 145 | 9.91e-56 | 188 |
| Msa0039170 | MtrunA17_Chr4g0037251 | 59.259 | 135 | 53 | 1 | 737 | 869 | 8 | 142 | 1.79e-51 | 176 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0039170 | AT5G52380.1 | 57.249 | 269 | 87 | 9 | 1 | 261 | 1 | 249 | 7.81e-82 | 264 |
| Msa0039170 | AT4G25590.1 | 84.559 | 136 | 21 | 0 | 735 | 870 | 2 | 137 | 5.58e-74 | 238 |
| Msa0039170 | AT4G25590.2 | 83.846 | 130 | 21 | 0 | 741 | 870 | 1 | 130 | 7.69e-73 | 235 |
| Msa0039170 | AT1G01750.2 | 77.143 | 140 | 30 | 1 | 732 | 869 | 4 | 143 | 6.93e-71 | 230 |
| Msa0039170 | AT1G01750.1 | 78.832 | 137 | 27 | 1 | 735 | 869 | 2 | 138 | 8.67e-71 | 230 |
| Msa0039170 | AT5G52360.1 | 82.353 | 136 | 24 | 0 | 735 | 870 | 2 | 137 | 1.16e-70 | 229 |
| Msa0039170 | AT3G46010.2 | 75.362 | 138 | 32 | 1 | 734 | 869 | 12 | 149 | 3.08e-70 | 229 |
| Msa0039170 | AT3G46010.1 | 75.912 | 137 | 31 | 1 | 735 | 869 | 2 | 138 | 6.02e-70 | 228 |
| Msa0039170 | AT4G00680.1 | 77.206 | 136 | 29 | 1 | 735 | 868 | 2 | 137 | 3.67e-69 | 225 |
| Msa0039170 | AT3G46000.1 | 74.627 | 134 | 34 | 0 | 735 | 868 | 2 | 135 | 4.95e-69 | 225 |
Find 191 sgRNAs with CRISPR-Local
Find 1106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGGAGTACATGGAG+AGG | 0.583802 | 1_1:+68717124 | Msa0039170:CDS |
| AAAAGAAGGAGTACATGGAG+AGG | 0.583802 | 1_1:+68717304 | Msa0039170:CDS |
| AAAAGAATAAGATATGTTTA+CGG | 0.452361 | 1_1:+68707671 | Msa0039170:CDS |
| AAACCCTAATCCTCAGCCTC+AGG | 0.549833 | 1_1:+68716603 | Msa0039170:CDS |
| AAAGCTTAAATCTCTCTCCA+AGG | 0.608046 | 1_1:+68717906 | Msa0039170:CDS |
| AAATGCTCAGTATATATAGC+AGG | 0.568557 | 1_1:+68717976 | Msa0039170:CDS |
| AAATTTGGTAACCTGACCAC+TGG | 0.493953 | 1_1:-68710633 | None:intergenic |
| AACAGAACCCTCAACAGACC+CGG | 0.570135 | 1_1:+68717419 | Msa0039170:CDS |
| AACAGACCCGGCTGTTGTAA+TGG | 0.439053 | 1_1:+68717431 | Msa0039170:CDS |
| AACCCCAATAGTCACAGATC+TGG | 0.542198 | 1_1:+68717242 | Msa0039170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATAAGATATGTTTACGGTGT+CGG | + | chr1_1:68707677-68707696 | Msa0039170:CDS | 30.0% | |
| GAACACTGACACATTAATAT+TGG | + | chr1_1:68708180-68708199 | Msa0039170:intron | 30.0% | |
| GTTATTTAGATTACACTGAG+TGG | + | chr1_1:68709110-68709129 | Msa0039170:intron | 30.0% | |
| AATTGTGGAATGGAAAATAG+CGG | - | chr1_1:68709350-68709369 | None:intergenic | 30.0% | |
| GTTCAAAATACATACACACA+AGG | - | chr1_1:68709428-68709447 | None:intergenic | 30.0% | |
| AAAAAAAATAGTTGGTTGCC+TGG | - | chr1_1:68709529-68709548 | None:intergenic | 30.0% | |
| AAATAACGAAGAAAAGTGGT+TGG | - | chr1_1:68709571-68709590 | None:intergenic | 30.0% | |
| AACCAAATAACGAAGAAAAG+TGG | - | chr1_1:68709575-68709594 | None:intergenic | 30.0% | |
| AGGAAATCTATCAGTACTTT+CGG | - | chr1_1:68709795-68709814 | None:intergenic | 30.0% | |
| GCACTTTAAATGTAACTTCT+TGG | + | chr1_1:68710362-68710381 | Msa0039170:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 68707325 | 68723058 | 68707325 | ID=Msa0039170;Name=Msa0039170 |
| chr1_1 | mRNA | 68707325 | 68723058 | 68707325 | ID=Msa0039170-mRNA-1;Parent=Msa0039170;Name=Msa0039170-mRNA-1;_AED=0.16;_eAED=0.16;_QI=81|0.44|0.4|0.9|0.66|0.8|10|0|870 |
| chr1_1 | exon | 68707325 | 68707912 | 68707325 | ID=Msa0039170-mRNA-1:exon:22475;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68707993 | 68708101 | 68707993 | ID=Msa0039170-mRNA-1:exon:22476;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68710612 | 68710694 | 68710612 | ID=Msa0039170-mRNA-1:exon:22477;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68716466 | 68717629 | 68716466 | ID=Msa0039170-mRNA-1:exon:22478;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68717859 | 68717997 | 68717859 | ID=Msa0039170-mRNA-1:exon:22479;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68718878 | 68718942 | 68718878 | ID=Msa0039170-mRNA-1:exon:22480;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68719293 | 68719378 | 68719293 | ID=Msa0039170-mRNA-1:exon:22481;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68720289 | 68720337 | 68720289 | ID=Msa0039170-mRNA-1:exon:22482;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68722081 | 68722340 | 68722081 | ID=Msa0039170-mRNA-1:exon:22483;Parent=Msa0039170-mRNA-1 |
| chr1_1 | exon | 68722908 | 68723058 | 68722908 | ID=Msa0039170-mRNA-1:exon:22484;Parent=Msa0039170-mRNA-1 |
| chr1_1 | five_prime_UTR | 68707325 | 68707405 | 68707325 | ID=Msa0039170-mRNA-1:five_prime_utr;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68707406 | 68707912 | 68707406 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68707993 | 68708101 | 68707993 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68710612 | 68710694 | 68710612 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68716466 | 68717629 | 68716466 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68717859 | 68717997 | 68717859 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68718878 | 68718942 | 68718878 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68719293 | 68719378 | 68719293 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68720289 | 68720337 | 68720289 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68722081 | 68722340 | 68722081 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| chr1_1 | CDS | 68722908 | 68723058 | 68722908 | ID=Msa0039170-mRNA-1:cds;Parent=Msa0039170-mRNA-1 |
| Gene Sequence |
>Msa0039170 ATGGTGAGCCAAAGACAACGACTCGCTAAGAAACGCTACAAAGAAGAACACCCCGAACTCTTCCCCAAAGTCGAACCCACTCCACCAAAAGACCCATCAAAAAAGAAGAAAAACAAATTCAAGCGCAAAAAACCCGACTCAAACTCCAAACCCCGAACCGGAAAACGCCCCCTTCGTGTTCCCGGTATGAAACCCGGCGAGAGCTGTTATATTTGCAAGGGATTAGACCACATCGCTAAATTCTGTCCTCAAAAAGCTGAATGGGAAAAGAATAAGATATGTTTACGGTGTCGGCGTCGCGGTCATCGAGCTCAGAATTGTCCTGATGGTGGTTCTAAAGAGGATTTTAAGTATTGTTATAATTGTGGTGATAATGGTCACTCTCTTGCTAACTGTCCTCATCCTCTTCAGGAAGGTGGGACTATGTTTGCTCAGTGTTTTGTTTGTAAGGAACAAGGGCATTTGAGTAAAAATTGCCCTAAAAATGCTCATGGCATTTATCCTAAGGGTGGTTGTTGTAAAATATGTGGTGGTGTCACACATTTGGCAAGGGATTGTCCTGATAAAGGGCAGAATGGATTTGGTGATGGTCGTCCTAAAGGGCCTGTTCATAACTTACTGAGAACCGACGAAAGGCCCAGTGGTCAGGTTACCAAATTTGTGAGTGGAGATGATATGGACGACGACTTCACAACAGAACGAATAGAACAAAAAATGCTGAGAGAAGAGAAACCAGAAGCATTTGCTTCACCGAAACAGAATACTGAAGATGATAGCAGTAAGAAGAAGAAGAAGAAGAGGTGTAGATGGAGAAAAAAGAAGGAGAAAATCAATGAAAACCCTAATCCTCAGCCTCAGGTATGTTCTGAACCCTTAGTAGTAGTTGCCCCAATCTCAGAACTCAAATCAAACGAAGAATCTGTTACTTTACCTGATTCAAATAATATCCCCGCGAAAAAGAATAGGAAGAACAGGAATAGGAATAAGAAGAAGAGCATGATCAATGGTGAAGAACCAAAACCTGCGTTAGAGCTAGGAAAGAATGAAGAACCAAAACCCGCTTTAGAGCTAGAAAAGAATGAAGAACCAAAACCCGCGTTAGAGCTAGAAAAGAATAAAGAAGTAGAATCAATTGAAGTTGCAAAAACTATGACTAGGAAGAGAAAACGAAAAAGCGTGCAAAATGGGGCAGAATCCAATGAGCATAATATTGAACAGGCTGAAACTGCGGTTCAGATGTCGATACTAGATACAGAAGTGCCTCTGATTGACAAAGCAATCGCGGTGAATAATCAACATGTAAGCTTAGATGAACTGGAAGAGAGGTACTTTGAGAGAAAAAAGACGAGGAAGGAAAAAAAGAAGGAGTACATGGAGAGGAAAAAACTGCAGGGTGAAGTCAAAACTAACGGGGAGGAAAAAACCAAGAACGAACCCTCTACAGACCCGTCTGTTGTAATGGACACTCACCCTGCAACCCCAATAGTCACAGATCTGGAAGAGAGGTACTTTGAGAGAAAAAAGACGAGGAAGGAAAAAAAGAAGGAGTACATGGAGAGGAAAAAACTGCAAGGTGAAGTCAAAACTAACGGGGAAGAAAAAACCAAGAACGATACACTTGAAACCCTAGCTCAGAGACCTTTTGACCCGATAACAGAACCCTCAACAGACCCGGCTGTTGTAATGGACACTCACCCTGCAACCCCAATAGTTGCAGAACAGAAGATTGCAAAGAAGAAACACAAGAAGAGGAAAAGGAAGTGTGAAGTGGGACAATATTCAATTGCTGAAACCTCAGTTCAGGGAACTAAAGGAATCACTGTCACTAATGCTCCCAATGATCAACATGGGCAAAGTTCTATGTTATGTTGGGCTTGCCGAGAGACCAATCACACCATACAACAATGCCAAAAGCTTAAATCTCTCTCCAAGGATGAGGAAGTTTGCTTCTTTTGTGGGGAAATCGGGCACTCACTTGGAAAATGCTCAGTATATATAGCAGAAGAGTCTTCCCTAAAAAAAAACTGGCAGAAGAGGCTACTTCCTCCACAAGGGGATTTTATTAGAGTGGAGGAAGACTTGCTAGGTGCTTATTTTGTAATGCACACGGGCACTTCAGCTATAACTGCCCTGGAAATTGCCATGATCCAAAGGGGTTGCCGGTGGAAGCTGAAAGCATGGTCCAGTGCTGAAGCTATAACTGCTAACGCAGCATCTGGAATGGCTGTTAATGATGAATGTAAATTGAAGTTTCTTGAGTTAAAAGCAAAGAGAAACTATCGTTTTATTGTGTTTAAGATTGAGAATCAGGAAGTGGTACTAGAGAAACTTGGAGGTCCAGAAGAAACCTATGAGGACTTTAGTGCATGTCTACCTGCTGATGAGTGTCGTTATGCAGTCTTTGATTTTGATTTCACCACTGCTGAAAACTGCATGAAAAGCAAGATTTTCTTCATTGCATGGTCTCCGGATATATCAAAGGTGAGACATAAGATGGTCTATGCTAGCACCAAGGACAGATTCAAGAGGGAACTTGATGGCATTCAAGTTGAGCTGCAAGCAACTGATCCTAGTGAAATGAGCTTTGATATCATAAAATCACGTGCACTATAA |
| Protein sequence |
>Msa0039170 MVSQRQRLAKKRYKEEHPELFPKVEPTPPKDPSKKKKNKFKRKKPDSNSKPRTGKRPLRVPGMKPGESCYICKGLDHIAKFCPQKAEWEKNKICLRCRRRGHRAQNCPDGGSKEDFKYCYNCGDNGHSLANCPHPLQEGGTMFAQCFVCKEQGHLSKNCPKNAHGIYPKGGCCKICGGVTHLARDCPDKGQNGFGDGRPKGPVHNLLRTDERPSGQVTKFVSGDDMDDDFTTERIEQKMLREEKPEAFASPKQNTEDDSSKKKKKKRCRWRKKKEKINENPNPQPQVCSEPLVVVAPISELKSNEESVTLPDSNNIPAKKNRKNRNRNKKKSMINGEEPKPALELGKNEEPKPALELEKNEEPKPALELEKNKEVESIEVAKTMTRKRKRKSVQNGAESNEHNIEQAETAVQMSILDTEVPLIDKAIAVNNQHVSLDELEERYFERKKTRKEKKKEYMERKKLQGEVKTNGEEKTKNEPSTDPSVVMDTHPATPIVTDLEERYFERKKTRKEKKKEYMERKKLQGEVKTNGEEKTKNDTLETLAQRPFDPITEPSTDPAVVMDTHPATPIVAEQKIAKKKHKKRKRKCEVGQYSIAETSVQGTKGITVTNAPNDQHGQSSMLCWACRETNHTIQQCQKLKSLSKDEEVCFFCGEIGHSLGKCSVYIAEESSLKKNWQKRLLPPQGDFIRVEEDLLGAYFVMHTGTSAITALEIAMIQRGCRWKLKAWSSAEAITANAASGMAVNDECKLKFLELKAKRNYRFIVFKIENQEVVLEKLGGPEETYEDFSACLPADECRYAVFDFDFTTAENCMKSKIFFIAWSPDISKVRHKMVYASTKDRFKRELDGIQVELQATDPSEMSFDIIKSRAL* |
