AlfalfaGEDB | Msa0817460

M. sativa cultivar zhongmu-4 / Msa0817460

KEGG: -
GO: -
PFAM: PF04788.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817460	XP_003616538.1	98.578	422	6	0	1	422	1	422	0.0	861

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817460	G7KBU0	98.578	422	6	0	1	422	1	422	0.0	861

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002680	Msa0817460	0.801049	1.081376e-48	-8.615850e-47
Msa0002710	Msa0817460	0.826620	2.454297e-54	-8.615850e-47
Msa0003400	Msa0817460	0.813916	2.009257e-51	-8.615850e-47
Msa0003720	Msa0817460	0.803728	3.034701e-49	-8.615850e-47
Msa0003890	Msa0817460	0.847092	1.439348e-59	-8.615850e-47
Msa0005270	Msa0817460	0.833103	6.462192e-56	-8.615850e-47
Msa0006110	Msa0817460	0.854487	1.191681e-61	-8.615850e-47
Msa0007440	Msa0817460	0.848247	6.922965e-60	-8.615850e-47
Msa0007990	Msa0817460	0.844063	9.534463e-59	-8.615850e-47
Msa0008220	Msa0817460	0.802008	6.878121e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817460	MtrunA17_Chr5g0436191	98.578	422	6	0	1	422	1	422	0.0	861
Msa0817460	MtrunA17_Chr3g0112781	71.264	435	105	6	1	416	1	434	0.0	623
Msa0817460	MtrunA17_Chr1g0154851	52.344	384	163	8	1	378	1	370	1.23e-135	394
Msa0817460	MtrunA17_Chr3g0141481	52.442	389	157	11	1	378	8	379	5.11e-134	390
Msa0817460	MtrunA17_Chr5g0403841	50.262	382	165	6	1	373	10	375	1.79e-131	383
Msa0817460	MtrunA17_Chr1g0207581	43.627	408	210	7	14	419	74	463	4.49e-113	339
Msa0817460	MtrunA17_Chr1g0152841	47.950	317	157	2	58	374	113	421	1.15e-107	325
Msa0817460	MtrunA17_Chr3g0134521	46.906	307	155	2	68	374	93	391	7.91e-103	311
Msa0817460	MtrunA17_Chr2g0309941	42.857	371	194	6	14	373	17	380	2.59e-98	299
Msa0817460	MtrunA17_Chr1g0176101	40.938	320	169	4	58	376	50	350	2.23e-82	257

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0817460	AT5G05840.1	61.827	427	143	6	1	408	1	426	0.0	514
Msa0817460	AT3G55720.1	53.935	432	175	11	1	409	1	431	1.48e-147	427
Msa0817460	AT1G75160.1	52.239	402	158	9	1	377	3	395	2.63e-131	384
Msa0817460	AT5G66740.1	50.526	380	169	6	1	374	1	367	4.23e-131	382
Msa0817460	AT3G19540.1	49.684	316	150	2	59	374	129	435	1.00e-107	327
Msa0817460	AT1G49840.1	41.063	414	223	6	6	409	78	480	3.54e-107	325
Msa0817460	AT1G27690.1	43.717	382	198	6	6	378	41	414	2.46e-105	318
Msa0817460	AT1G79420.1	38.978	372	198	8	29	376	36	402	2.29e-90	280
Msa0817460	AT5G06610.1	41.121	321	168	8	59	376	51	353	3.36e-78	247

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Find 107 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAATATGTGGCAGCTG+TGG	0.539484	5_3:-52761764	Msa0817460:CDS
AAAATTAGTGCAGGGAGTGA+TGG	0.488027	5_3:-52761526	Msa0817460:CDS
AAACAATATGTGGCAGCTGT+GGG	0.574423	5_3:-52761763	Msa0817460:CDS
AAACGTTTAAGTGGCCTAGG+TGG	0.667507	5_3:+52761451	None:intergenic
AAACTTGGGTCACTTTATCA+AGG	0.484471	5_3:-52759824	Msa0817460:CDS
AAATGAGGTTTGGAAAATTG+AGG	0.496792	5_3:-52759763	Msa0817460:CDS
AACTCTAAACACCACAATTC+TGG	0.410084	5_3:+52761565	None:intergenic
AAGAGTTGAATATTTGTGTT+TGG	0.407599	5_3:+52762214	None:intergenic
AAGGACCTGAGAATCATTCA+AGG	0.576245	5_3:-52759794	Msa0817460:CDS
AATAATGAGAATATGTATCT+TGG	0.376796	5_3:+52762455	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGTCTAGTTAAGATCGAA+AGG	+	chr5_3:52759477-52759496	None:intergenic	30.0%
TGTGTCATCACATCATATTT+AGG	+	chr5_3:52759589-52759608	None:intergenic	30.0%
GATGATCTTGATTCATTGTT+TGG	+	chr5_3:52759888-52759907	None:intergenic	30.0%
ACAAATATTCAACTCTTGCT+TGG	-	chr5_3:52759927-52759946	Msa0817460:CDS	30.0%
TTAATGTTACGAGTGATGAT+GGG	+	chr5_3:52760002-52760021	None:intergenic	30.0%
TTTAATGTTACGAGTGATGA+TGG	+	chr5_3:52760003-52760022	None:intergenic	30.0%
ATCATCACTCGTAACATTAA+AGG	-	chr5_3:52760002-52760021	Msa0817460:CDS	30.0%
ATTATTAGGGTTATGCTAAC+CGG	+	chr5_3:52760789-52760808	None:intergenic	30.0%
AAGGGAAAACAAAGTGAAAT+GGG	+	chr5_3:52761097-52761116	None:intergenic	30.0%
TAAGGGAAAACAAAGTGAAA+TGG	+	chr5_3:52761098-52761117	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	52759464	52762693	52759464	ID=Msa0817460;Name=Msa0817460
chr5_3	mRNA	52759464	52762693	52759464	ID=Msa0817460-mRNA-1;Parent=Msa0817460;Name=Msa0817460-mRNA-1;_AED=0.09;_eAED=0.09;_QI=241\|1\|1\|1\|0.66\|0.5\|4\|90\|422
chr5_3	exon	52759464	52760174	52759464	ID=Msa0817460-mRNA-1:exon:15862;Parent=Msa0817460-mRNA-1
chr5_3	exon	52761444	52761812	52761444	ID=Msa0817460-mRNA-1:exon:15861;Parent=Msa0817460-mRNA-1
chr5_3	exon	52762123	52762571	52762123	ID=Msa0817460-mRNA-1:exon:15860;Parent=Msa0817460-mRNA-1
chr5_3	exon	52762623	52762693	52762623	ID=Msa0817460-mRNA-1:exon:15859;Parent=Msa0817460-mRNA-1
chr5_3	five_prime_UTR	52762623	52762693	52762623	ID=Msa0817460-mRNA-1:five_prime_utr;Parent=Msa0817460-mRNA-1
chr5_3	five_prime_UTR	52762402	52762571	52762402	ID=Msa0817460-mRNA-1:five_prime_utr;Parent=Msa0817460-mRNA-1
chr5_3	CDS	52762123	52762401	52762123	ID=Msa0817460-mRNA-1:cds;Parent=Msa0817460-mRNA-1
chr5_3	CDS	52761444	52761812	52761444	ID=Msa0817460-mRNA-1:cds;Parent=Msa0817460-mRNA-1
chr5_3	CDS	52759554	52760174	52759554	ID=Msa0817460-mRNA-1:cds;Parent=Msa0817460-mRNA-1
chr5_3	three_prime_UTR	52759464	52759553	52759464	ID=Msa0817460-mRNA-1:three_prime_utr;Parent=Msa0817460-mRNA-1

Gene Sequence

>Msa0817460

ATGAAGAAGCTTTGTCCCAACCTTTATCATGAGGATGGCCTTGAAACAGTGCTAGAAGTTCCCATCCCAGAAGATATTTTCATCAACAAGAGTGGAACCACATGGAAGAACATGAAGCAATGGATGAAACCAAACAATGAATCAAGATCATCACGTTTTGAAGATCCAAACACAAATATTCAACTCTTGCTTGGTGTTGTAGGAGCTCCATTGATTCCTTTTCCTATCACCAATGAGTACAAACCCATCATCACTCGTAACATTAAAGGCCATAACATTGAGGCGTCGATGGCGAAGTATATAGTAAAACAATATGTGGCAGCTGTGGGAGGAGAGAGTGTTTTAAATAATGTTGAAAGTATGTATGCAATGGGTGAAGTGAGAATAGGGACCTCAGAGTTTGTTGCAGGGGAAGGGGGTGTGAATAGTAAGAAGATGGTGAAGAAAGTGAAGAAGGTGGATATGAAAGAAGAGTTGGGAGGTTTTGTGTTGTGGCAAAAGAGACCAGAATTGTGGTGTTTAGAGTTGGTGGTTTCTGGGTACAAAATTAGTGCAGGGAGTGATGGAAAAGTGGCTTGGAGGCAAACACCTTGGCATCATTCTCATGCTTCTAGGGGTCCACCTAGGCCACTTAAACGTTTACTTCAGGGTCTTGATCCAAAATCCACTGCCAATCTATTCAACAACTCAACATGCATAGGAGAGAAAGCAGTAAACAACGAAGAATGTTTCATTCTAAAACTTGAAGCAGAATCCACTTCATTACAAACAAGAAGCAGTAGCAACATAGAAATAATTGGTCACACAGTTTTAGGCTATTTCAGCCAAAGAACTGGACTTTTGGTTCAATTAGAAGACACCAACTTGATTAAACTAAAATCTTCTGAAACTGAATTCATATTTTGGGAAACAAATACAGAATCTCTCATACAAGACTATAGAATAGTGGATGGTATTCAAATAGCACATTGTGGGAAAACTTGGGTCACTTTATCAAGGTTTGGTGAAGGACCTGAGAATCATTCAAGGACAAGTATAAATGAGGTTTGGAAAATTGAGGAAGTTGATTTTAATATTAAAGGGTTGTCTTTGGATTGTTTTTTACCTCCTAGTGATTTGAAGAGAGATGAAGAAAAAGGAGTGGTTTCTGTTAGTAATGCTAAGTTACCATATAAAATTCAATCTGCTTCTTTTAAGATTAGTGCTTCCAAAGTAGCTGCTATTGATGTGGATGATTCATGTGTTAGTGATAACATTCAAGATATGTAG

Protein sequence

>Msa0817460

MKKLCPNLYHEDGLETVLEVPIPEDIFINKSGTTWKNMKQWMKPNNESRSSRFEDPNTNIQLLLGVVGAPLIPFPITNEYKPIITRNIKGHNIEASMAKYIVKQYVAAVGGESVLNNVESMYAMGEVRIGTSEFVAGEGGVNSKKMVKKVKKVDMKEELGGFVLWQKRPELWCLELVVSGYKISAGSDGKVAWRQTPWHHSHASRGPPRPLKRLLQGLDPKSTANLFNNSTCIGEKAVNNEECFILKLEAESTSLQTRSSSNIEIIGHTVLGYFSQRTGLLVQLEDTNLIKLKSSETEFIFWETNTESLIQDYRIVDGIQIAHCGKTWVTLSRFGEGPENHSRTSINEVWKIEEVDFNIKGLSLDCFLPPSDLKRDEEKGVVSVSNAKLPYKIQSASFKISASKVAAIDVDDSCVSDNIQDM*