AlfalfaGEDB | Msa0936060

M. sativa cultivar zhongmu-4 / Msa0936060

KEGG: K05658
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF00664.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936060	sp\|Q9LHD1\|AB15B_ARATH	68.038	1264	369	8	1	1254	1	1239	0.0	1744

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936060	G7KJG7	92.885	1265	87	1	1	1265	1	1262	0.0	2386

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010890	Msa0936060	0.812751	3.621310e-51	-8.615850e-47
Msa0029240	Msa0936060	0.809659	1.696475e-50	-8.615850e-47
Msa0036140	Msa0936060	0.815980	7.001149e-52	-8.615850e-47
Msa0051560	Msa0936060	0.803965	2.709228e-49	-8.615850e-47
Msa0051680	Msa0936060	0.800331	1.515123e-48	-8.615850e-47
Msa0079070	Msa0936060	0.806853	6.722044e-50	-8.615850e-47
Msa0108410	Msa0936060	0.801653	8.136493e-49	-8.615850e-47
Msa0160960	Msa0936060	0.802053	6.733947e-49	-8.615850e-47
Msa0172080	Msa0936060	0.805224	1.480309e-49	-8.615850e-47
Msa0195100	Msa0936060	0.814655	1.379108e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0936060	MtrunA17_Chr6g0451881	92.885	1265	87	1	1	1265	1	1262	2386
Msa0936060	MtrunA17_Chr6g0452041	82.745	1275	209	3	1	1265	1	1274	2174
Msa0936060	MtrunA17_Chr6g0452171	83.777	1276	195	3	1	1265	1	1275	2144
Msa0936060	MtrunA17_Chr6g0452141	81.348	1276	223	5	1	1265	1	1272	2103
Msa0936060	MtrunA17_Chr6g0452111	80.584	1267	235	4	6	1266	13	1274	2100
Msa0936060	MtrunA17_Chr6g0451911	79.416	1268	255	4	1	1263	1	1267	2041
Msa0936060	MtrunA17_Chr7g0233901	66.241	1253	401	6	7	1253	3	1239	1711
Msa0936060	MtrunA17_Chr4g0041871	52.195	1253	571	10	23	1255	5	1249	1289
Msa0936060	MtrunA17_Chr6g0486841	49.841	1254	611	7	1	1251	1	1239	1236
Msa0936060	MtrunA17_Chr6g0452061	77.564	780	161	5	492	1265	13	784	1217

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0936060	AT3G28345.1	68.038	1264	369	8	1	1254	1	1239	0.0	1744
Msa0936060	AT3G28390.1	65.643	1237	408	5	16	1252	3	1222	0.0	1696
Msa0936060	AT3G28415.2	65.319	1237	417	4	16	1252	2	1226	0.0	1687
Msa0936060	AT3G28380.1	64.326	1253	430	7	1	1252	1	1237	0.0	1685
Msa0936060	AT3G28380.1	36.738	607	367	10	658	1255	3	601	2.51e-105	362
Msa0936060	AT3G28360.1	64.701	1238	422	5	16	1253	2	1224	0.0	1660
Msa0936060	AT3G28415.1	64.673	1237	417	5	16	1252	2	1218	0.0	1658
Msa0936060	AT3G28860.1	44.480	1259	669	14	5	1251	8	1248	0.0	1074
Msa0936060	AT2G36910.1	43.331	1267	695	10	2	1252	5	1264	0.0	1030
Msa0936060	AT2G36910.1	38.219	539	329	3	717	1255	77	611	8.35e-112	381

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Find 207 sgRNAs with CRISPR-Local

Find 483 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAGTGGCATTAGTAGG+AGG	0.609878	6_3:+6688442	Msa0936060:CDS
AAACACACTTGCTTCCACTC+TGG	0.531701	6_3:-6690535	None:intergenic
AAAGATAGATTCTGAAAACA+TGG	0.593592	6_3:+6688300	Msa0936060:CDS
AAAGGGATGGTGACAATTGA+TGG	0.431866	6_3:+6692042	Msa0936060:CDS
AAATTATAGAGAAAGTCTTG+GGG	0.685754	6_3:+6688329	Msa0936060:CDS
AACAACACCACTGCTTCCTA+TGG	0.481536	6_3:-6687807	None:intergenic
AACAATACAGGTAAGATCTT+TGG	0.298105	6_3:+6691231	Msa0936060:intron
AACTCAGTTGGTAGTGCTTC+TGG	0.221752	6_3:+6685073	Msa0936060:CDS
AAGATCAAACAATATAATTG+CGG	0.615473	6_3:+6692602	Msa0936060:CDS
AAGATCATTAGTCATGCTAC+TGG	0.524550	6_3:-6691743	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTGATAAACAACACCAAA+GGG	-	chr6_3:6685042-6685061	None:intergenic	30.0%
TGTAAAATAGTCTCCGTATT+TGG	-	chr6_3:6685460-6685479	None:intergenic	30.0%
AACAAAAAGAAGAATGGGAA+AGG	+	chr6_3:6685745-6685764	Msa0936060:intron	30.0%
ACAAAAAGAAGAATGGGAAA+GGG	+	chr6_3:6685746-6685765	Msa0936060:intron	30.0%
AAATAAAGTCATAGGCTCAT+AGG	-	chr6_3:6685789-6685808	None:intergenic	30.0%
CAAAATTTATAAAGGGAGAG+TGG	-	chr6_3:6685872-6685891	None:intergenic	30.0%
TAAGACACCCTCAAATTTAT+CGG	-	chr6_3:6685939-6685958	None:intergenic	30.0%
ACATATTTCCCTTAAGTTAG+GGG	-	chr6_3:6686097-6686116	None:intergenic	30.0%
GACATATTTCCCTTAAGTTA+GGG	-	chr6_3:6686098-6686117	None:intergenic	30.0%
AGACATATTTCCCTTAAGTT+AGG	-	chr6_3:6686099-6686118	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6_3	gene	6684890	6692659	6684890	ID=Msa0936060;Name=Msa0936060
chr6_3	mRNA	6684890	6692659	6684890	ID=Msa0936060-mRNA-1;Parent=Msa0936060;Name=Msa0936060-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|0.16\|0\|1\|1\|1\|7\|0\|1270
chr6_3	exon	6684890	6685132	6684890	ID=Msa0936060-mRNA-1:exon:2153;Parent=Msa0936060-mRNA-1
chr6_3	exon	6686819	6686873	6686819	ID=Msa0936060-mRNA-1:exon:2154;Parent=Msa0936060-mRNA-1
chr6_3	exon	6686967	6687145	6686967	ID=Msa0936060-mRNA-1:exon:2155;Parent=Msa0936060-mRNA-1
chr6_3	exon	6687482	6687939	6687482	ID=Msa0936060-mRNA-1:exon:2156;Parent=Msa0936060-mRNA-1
chr6_3	exon	6688210	6688750	6688210	ID=Msa0936060-mRNA-1:exon:2157;Parent=Msa0936060-mRNA-1
chr6_3	exon	6689971	6690888	6689971	ID=Msa0936060-mRNA-1:exon:2158;Parent=Msa0936060-mRNA-1
chr6_3	exon	6691241	6692659	6691241	ID=Msa0936060-mRNA-1:exon:2159;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6684890	6685132	6684890	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6686819	6686873	6686819	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6686967	6687145	6686967	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6687482	6687939	6687482	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6688210	6688750	6688210	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6689971	6690888	6689971	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1
chr6_3	CDS	6691241	6692659	6691241	ID=Msa0936060-mRNA-1:cds;Parent=Msa0936060-mRNA-1

Gene Sequence

>Msa0936060

ATGGGTGGTGGTGATGAGAAAAATGTTTCCATTAATGTTAAGAAAAAGAAAAATGGTTCTTTAAAGTCTATTTTCATGCATGCTGATGTTCTAGATTGGTTTTTCATGGCTTTTGGTTTTTTTGGGGCTATTGGTGATGGCATGATGGTCCCTTTGGTGTTGTTTATCACAAGCAAAATTTTGAACTCAGTTGGTAGTGCTTCTGGAACATCAAGCAGCAATTTTGTCCATGACATCAATAAGAATGCAGTTGTTGTGTTATACATGGCTTGTGCTTCTTTTTTTGTTTGCTTCCTAGAGGGTTATTGTTGGACAAGGACAGGTGAAAGACAAGCAGCAAGAATGAGAGCTAGGTATCTAAAAGCAGTTCTAAGACAAGAAGTAGCATACTTTGATTTGCATGTTACAAGCACAACAGATGTCATCACTAGTGTATCTAGTGATAGCCTAGTAATTCAAGATGTTCTTAGTGACAAGGTTCCAAATTTTTTGATGAATGCATCTAGGTTCCTTGCAAGCAATATAGTGGCATTTGCATTATTATGGAGATTGGCTATTGTTGGATTCCCTTTTATGGTTCTACTTGTGATTCCTGGTTACATGTATAAGAAGACTTCGATGAGGTTGGCTAGAAAGCTCAGAGAAGAGTACAATCAAGCTGGTACAATAGCAGAACAAGCAATATCTTCTATAAGAACTGTTTATTCTTTTGTAGGGGAAAGCAAAACTGTAGCAGCTTTCTCTAATGCTTTGGAAGGGTCAGTGAAATTAGGTTTGAAACAAGGTTTGGCTAAAGGATTTGCCATAGGAAGCAGTGGTGTTGTTTTTGCTATATGGTCTTTAATATTCTATTATGGTAGCATAATGGTCATGTATCATGGTGCTAAAGGTGGTACTGTATTTGCAGTTGGAATAACATTAGCAATTGGTGGATTGGCTTTTGGATCTTGTTTCTCAAATGTGAGGTACTTCGCAGAAGCAAGTGTTGCAGGAGAAAGAATAATGGAAGTGATAAAAAGAGTTCCAAAGATAGATTCTGAAAACATGGAAGGTGAAATTATAGAGAAAGTCTTGGGGGAAGTTGAGTTCAAAAATGTTGAATTTGTGTATCCATCAAGACCAGAAAGTGTGATCTTAAATGATTTTTGCTTAAAGGTTCCATCAGGGAAAACAGTGGCATTAGTAGGAGGAAGTGGTTCAGGAAAATCAACAGTTGTATCACTTTTGCAAAGATTTTATGATCCAATTGGTGGAGAGATTCTTCTTGATCGAGTTGCTATTCATAAGTTGCAACTTAAGTGGCTTAGGTCACAAATGGGTTTAGTGAGTCAAGAACCTGCTTTGTTTGCAACAAGCATCAAGGAGAATATACTTTTTGGTAGAGAGGATGCTACATTTGAAGATGTTGTTGATGCTGCTAAAGCTTCAAATGCTCATAATTTCATTTCAATGTTGCCACAAGGATATGATACTCAGGTTGGGGAGAGGGGAATTCAAATGTCTGGGGGACAAAAGCAAAGGATTGCCATTGCAAGAGCCATAGTAAAAATGCCAAAAATTCTATTGCTAGACGAAGCAACAAGTGCACTCGATTCTGAATCCGAACGAGTTGTTCAAGAAGCTCTAGACAAAGCTGTTGTTGGACGCACCACCATCATCATCGCTCACCGCTTATCCACCATCCAAAATGCAGACATCATTGCAGTTGTTCAAAATGGTAAGATCATGGAAATAGGATCTCACGAAAGCCTAATGCAAAATGACAATTCCATTTACACATCCCTAGTTCATCTCCAACACACCAAAAATGACCAAAACGACGACACCCTATCTATCATGAGTAGACATCACATTAGTTGTAGATTTATGAGTCATTCTAGCTCTTTCAACTCAATGACACATGGTGGTGATGATGTTGTTAATTATGACAATGATGTTGAAGATGTTGTTGACGATGTTGATAATAATAATAAGAAGAAGAGGAAGAAGAAGAAAAAGGTGAAGGTTCCTTCATTTCGAAGATTGTTGGCAATGAATGTTCCAGAGTGGAAGCAAGTGTGTTTGGGGTGTTTGAATGCGGTTTTATTCGGTGCGGTTCAACCGATATCGGCATTTGCAACGGGGGCAATTGCATCAGTTTATTTTCTGAATGACCATGATGAGATGAAGAAGCAGATTAGGATATATGCTTTTACTTTTCTAGGGTTGGCTTTGGTCTCTATTGTTTCCAATATGCTTCAACATTATAGCTTTGCTTACATGGGAGAGTACTTGACTAAGAGGATTAGAGAAAGAATGTTTTACAAAATACTCACTTTTGAAGTTGGATGGTTTGATGAGGATCAAAATTCTACGGGTGCTATTTGCTCTAGACTTGCCAAAGAAGGCAACGTGGTAAGATCTTTGGTGGGTGATAGTTTGTCACTAGTGGTACAAACTATATCAGCAATGGTAGTAACTTTTACAATAGGTCTAATCATTACGTGGAGACTATCCATTGTTATGATAGTAGTTCAACCAATTACCATTTTTTGCTTCTACACAAGACGTGTTCTTCTTAATAACATGTCAAGTAAGGCCATTAAGGCTCAAAATGAAAGTAGTAAAATTGCTGCTGAAGCTGTTTCAAATCTAAGAACCATCACATCTTTTTCATCACAAAATAGAATCCTCAAAATGCTTAAAAAAGCCCAACAAGGACCACGTCATGAAAGTATTAGACAATCTTGGTTTGCAGGTATTGGGCTTGCATGTGCACAAAGCCTCATTTTTTGTACTAGAGCCTTAAACTTTTGGTATGGTGGAAAACTTGTCTCACATGGATATATAACAAAAAATCAATTTTTTGAGACAGTTATGATTTGGATAAGCATTGGAAAAGTTATTGCAGATGCTGCCAGTAGCATGACTAATGATCTTGCTAAAGGTTCTGATGCCGTCGGATCGGTTTTTGCAATTCTTGATAGGGATACGAAAATCAAGTCGGACGACTTAGAAGGTTTCAAAGCTGAAAAGTTGATAGGAAAAATTGTATTTCATGATGTACATTTTGCATATCCGGCTAGGCCTAGTGCGATGGTATTTCAAGGATTCTCTATTGAAATTGACGCTGGGAAATCAACGGCTTTGGTTGGGGAAAGTGGTTCTGGAAAATCAACAATCATAGGACTAATTGAGAGGTTCTATGATCCACTTAAAGGGATGGTGACAATTGATGGTAGAGACATAAAGACTTATAATCTAAGGTCATTAAGGAAACATATTGCACTTGTGAGTCAAGAACCAACATTGTTTGGTGGTACCATAAGAGAAAATATTATATATGGAGCATATGATGATAAAGTTGATGAGAGTGAGATACTTGAGGCATCAAAGGCTGCAAATGCTCACGATTTCATATCAAGCTTAAAGGATGGTTATGACACACTGTGTGGTGATAGAGGAGTTCAACTTAGTGGTGGTCAAAAACAAAGAATTGCAATAGCTAGAGCTATATTGAAGAATCCAGAGGTGTTGCTTTTGGATGAAGCAACAAGTGCACTTGATAGTCAATCAGAGAAATTAGTCCAAGAAGCTTTAGAAAGGGTTATGGTTGGAAGGACAAGTGTGGTTGTAGCACATAGGTTGAGTACTATACAAAATTGTGATTTGATTGCTGTTTTGGATAAGGGAAGTGTTGTTGAGAAAGGAACACATTCATCTTTGTTGTCTAAAGGGTCAAGTGGAGCATATTACACTTTGGTAAGCCTACAAAGAAGATCAAACAATATAATTGCGGATTCTTCGCATGAAATTAAAAGTTCCCATGTTTGA

Protein sequence

>Msa0936060

MGGGDEKNVSINVKKKKNGSLKSIFMHADVLDWFFMAFGFFGAIGDGMMVPLVLFITSKILNSVGSASGTSSSNFVHDINKNAVVVLYMACASFFVCFLEGYCWTRTGERQAARMRARYLKAVLRQEVAYFDLHVTSTTDVITSVSSDSLVIQDVLSDKVPNFLMNASRFLASNIVAFALLWRLAIVGFPFMVLLVIPGYMYKKTSMRLARKLREEYNQAGTIAEQAISSIRTVYSFVGESKTVAAFSNALEGSVKLGLKQGLAKGFAIGSSGVVFAIWSLIFYYGSIMVMYHGAKGGTVFAVGITLAIGGLAFGSCFSNVRYFAEASVAGERIMEVIKRVPKIDSENMEGEIIEKVLGEVEFKNVEFVYPSRPESVILNDFCLKVPSGKTVALVGGSGSGKSTVVSLLQRFYDPIGGEILLDRVAIHKLQLKWLRSQMGLVSQEPALFATSIKENILFGREDATFEDVVDAAKASNAHNFISMLPQGYDTQVGERGIQMSGGQKQRIAIARAIVKMPKILLLDEATSALDSESERVVQEALDKAVVGRTTIIIAHRLSTIQNADIIAVVQNGKIMEIGSHESLMQNDNSIYTSLVHLQHTKNDQNDDTLSIMSRHHISCRFMSHSSSFNSMTHGGDDVVNYDNDVEDVVDDVDNNNKKKRKKKKKVKVPSFRRLLAMNVPEWKQVCLGCLNAVLFGAVQPISAFATGAIASVYFLNDHDEMKKQIRIYAFTFLGLALVSIVSNMLQHYSFAYMGEYLTKRIRERMFYKILTFEVGWFDEDQNSTGAICSRLAKEGNVVRSLVGDSLSLVVQTISAMVVTFTIGLIITWRLSIVMIVVQPITIFCFYTRRVLLNNMSSKAIKAQNESSKIAAEAVSNLRTITSFSSQNRILKMLKKAQQGPRHESIRQSWFAGIGLACAQSLIFCTRALNFWYGGKLVSHGYITKNQFFETVMIWISIGKVIADAASSMTNDLAKGSDAVGSVFAILDRDTKIKSDDLEGFKAEKLIGKIVFHDVHFAYPARPSAMVFQGFSIEIDAGKSTALVGESGSGKSTIIGLIERFYDPLKGMVTIDGRDIKTYNLRSLRKHIALVSQEPTLFGGTIRENIIYGAYDDKVDESEILEASKAANAHDFISSLKDGYDTLCGDRGVQLSGGQKQRIAIARAILKNPEVLLLDEATSALDSQSEKLVQEALERVMVGRTSVVVAHRLSTIQNCDLIAVLDKGSVVEKGTHSSLLSKGSSGAYYTLVSLQRRSNNIIADSSHEIKSSHV*