AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0053990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053990 MtrunA17_Chr1g0150561 97.767 403 9 0 13 415 1 403 0.0 809
Msa0053990 MtrunA17_Chr3g0122401 25.258 388 241 13 33 413 118 463 3.47e-30 121
Msa0053990 MtrunA17_Chr8g0347881 25.304 411 236 18 29 414 39 403 2.98e-20 92.0
Msa0053990 MtrunA17_Chr1g0161891 22.192 365 221 11 32 390 19 326 5.24e-18 84.7
Msa0053990 MtrunA17_Chr8g0347851 25.441 397 238 19 34 414 44 398 5.85e-13 70.1
Msa0053990 MtrunA17_Chr1g0161871 26.201 229 137 8 32 242 30 244 2.46e-12 68.2
Msa0053990 MtrunA17_Chr7g0220421 22.506 391 243 12 34 415 98 437 4.45e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0053990 AT4G33160.1 57.143 399 157 12 27 415 63 457 4.38e-146 423
Msa0053990 AT4G33160.2 57.143 399 157 12 27 415 124 518 1.35e-145 424
Msa0053990 AT1G27340.1 24.528 371 228 12 32 394 118 444 2.44e-26 110
Msa0053990 AT3G61590.4 24.651 430 254 22 14 414 58 446 4.60e-12 67.8
Msa0053990 AT3G61590.1 24.651 430 254 22 14 414 22 410 6.17e-12 67.4
Msa0053990 AT3G61590.2 23.953 430 257 20 14 414 30 418 7.19e-12 67.0
Msa0053990 AT3G61590.3 23.953 430 257 20 14 414 30 418 7.19e-12 67.0

Find 90 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAAAATGTTGCATTAA+TGG 0.164880 1_2:-8536104 Msa0053990:CDS
ATACAATCTTGTAGGATATT+TGG 0.194528 1_2:+8536738 None:intergenic
CAAACAGAAGCTTCTATTCT+TGG 0.227716 1_2:+8536044 None:intergenic
TCTTGGAAACCACAAGTCTT+AGG 0.228546 1_2:-8536322 Msa0053990:CDS
TCTCCATTAATGCAACATTT+TGG 0.236954 1_2:+8536101 None:intergenic
CACTTATGTTCTTTGTGATT+TGG 0.296690 1_2:-8536147 Msa0053990:CDS
ATTCCTGTTGCAGCTTCTAA+TGG 0.302810 1_2:-8536905 Msa0053990:CDS
CTAGCCGGATATCTTGGTTT+TGG 0.307552 1_2:-8536008 Msa0053990:three_prime_UTR
TGTTCTAAATTTCTGTTCAA+TGG 0.332421 1_2:-8537251 Msa0053990:CDS
ATTGTGTTGGTGCCGGTTCT+CGG 0.340920 1_2:-8536202 Msa0053990:CDS
GTAACCTTGTTCTCATTTAG+TGG 0.345733 1_2:-8535791 Msa0053990:three_prime_UTR
GTGATTTGGTAACCAACAAC+TGG 0.347069 1_2:-8536133 Msa0053990:CDS
CTGTCAACAATCAAATCATT+TGG 0.364285 1_2:+8535848 None:intergenic
ATAGACTTTAAACGAAAGAT+TGG 0.375910 1_2:+8536697 None:intergenic
GAAGACTAATACAATCTTGT+AGG 0.389829 1_2:+8536730 None:intergenic
TCATGCAACATAGCTAGATA+TGG 0.407514 1_2:-8535878 Msa0053990:three_prime_UTR
ATAACTCAAGCTCTTCTTGT+TGG 0.420790 1_2:-8536676 Msa0053990:CDS
TGTTGCAGCTTCTAATGGCC+TGG 0.422631 1_2:-8536900 Msa0053990:CDS
GAATAGAAGCTTCTGTTTGA+CGG 0.426490 1_2:-8536040 Msa0053990:exon
TAACTCAAGCTCTTCTTGTT+GGG 0.426988 1_2:-8536675 Msa0053990:CDS
TACAAGATTGTATTAGTCTT+CGG 0.428289 1_2:-8536728 Msa0053990:CDS
GAAATTCAACGGAGGTAGTT+CGG 0.429403 1_2:+8536817 None:intergenic
CTTGGAAACCACAAGTCTTA+GGG 0.431925 1_2:-8536321 Msa0053990:CDS
TGAAGTTTCCTGACGAAGTT+CGG 0.432189 1_2:+8536870 None:intergenic
TGGGAAGACGAGAATGCTCT+TGG 0.432787 1_2:-8536656 Msa0053990:CDS
GTTTGCTACCGAACTTCGTC+AGG 0.434074 1_2:-8536878 Msa0053990:CDS
AGTGTTTCTTTGAGTACCCT+AGG 0.452698 1_2:-8536424 Msa0053990:CDS
AATGCCCTAGCCGGATATCT+TGG 0.453017 1_2:-8536014 Msa0053990:three_prime_UTR
AAAAGAAAGTCTCCAGAGAA+TGG 0.455501 1_2:-8537217 Msa0053990:CDS
ACATCCCCACAAGGGATCCT+TGG 0.462778 1_2:-8537042 Msa0053990:CDS
TGGCAATTCAACCCAGTTGT+TGG 0.463420 1_2:+8536121 None:intergenic
AAACTACTGAATCCATTCTC+TGG 0.465062 1_2:+8537205 None:intergenic
AGAAGATCCGGTCACAGGAT+TGG 0.466882 1_2:+8536841 None:intergenic
GAATCTTCGAGCAGAAGATT+CGG 0.467832 1_2:+8536941 None:intergenic
TAGACTTTAAACGAAAGATT+GGG 0.470551 1_2:+8536698 None:intergenic
TCGATGAGACCAACCGATGC+TGG 0.476739 1_2:-8536289 Msa0053990:CDS
TTACTTCTGTGTGCAAAAGA+TGG 0.479574 1_2:-8537108 Msa0053990:CDS
ATGACAGACTGGTTGAGATC+GGG 0.482940 1_2:+8537004 None:intergenic
GATATCAATTGTGTTGGTGC+CGG 0.486697 1_2:-8536209 Msa0053990:CDS
GTACTCAAAGAAACACTTGC+AGG 0.487063 1_2:+8536430 None:intergenic
TTGACTCGGCTGAAAACAGT+TGG 0.492513 1_2:-8536979 Msa0053990:CDS
ATAACCAAAACCAAGATATC+CGG 0.496003 1_2:+8536004 None:intergenic
GAATGAAGATGTCTTTGAAA+AGG 0.497404 1_2:-8537167 Msa0053990:CDS
GATGACAGACTGGTTGAGAT+CGG 0.499005 1_2:+8537003 None:intergenic
AGGCCATTAGAAGCTGCAAC+AGG 0.501616 1_2:+8536902 None:intergenic
GCACCAACACAATTGATATC+CGG 0.505766 1_2:+8536212 None:intergenic
AGGTAGTTCGGTAGAAGATC+CGG 0.510241 1_2:+8536829 None:intergenic
CGAGAATGCTCTTGGGAGGA+AGG 0.510339 1_2:-8536648 Msa0053990:CDS
ACCGACGAATATTGCTTGGT+TGG 0.512080 1_2:+8536527 None:intergenic
AATGACCGACGAATATTGCT+TGG 0.513209 1_2:+8536523 None:intergenic
GTGTACTTTCTGAGCAAGGC+TGG 0.518608 1_2:-8536581 Msa0053990:CDS
ATTGATATCGTCGAGTGTAA+CGG 0.526717 1_2:-8536374 Msa0053990:CDS
TCTTTGAAAAGGTACTTTCG+TGG 0.527524 1_2:-8537156 Msa0053990:CDS
ACGAAGTTCGGTAGCAAACC+AGG 0.530457 1_2:+8536882 None:intergenic
CAGCAGCAATGTCTCATGAA+TGG 0.530978 1_2:-8536247 Msa0053990:CDS
AGAACATAAGTGCATAGCTC+AGG 0.532205 1_2:+8536158 None:intergenic
TGTTTGCAATTGAGGTCTCA+AGG 0.532935 1_2:-8537580 Msa0053990:intron
GGGAAGACGAGAATGCTCTT+GGG 0.541458 1_2:-8536655 Msa0053990:CDS
CTGCAATCTGTTGCCAGCAT+CGG 0.543898 1_2:+8536276 None:intergenic
TTGCATTAATGGAGAAGTCA+TGG 0.546480 1_2:-8536093 Msa0053990:CDS
TGACGGTGAAATGCCCTAGC+CGG 0.554753 1_2:-8536023 Msa0053990:three_prime_UTR
AATCTGTTGCCAGCATCGGT+TGG 0.557369 1_2:+8536280 None:intergenic
CCAGTCTGTCATCTTTGACT+CGG 0.557680 1_2:-8536993 Msa0053990:CDS
CAAAACCAAGATATCCGGCT+AGG 0.558173 1_2:+8536009 None:intergenic
TCGGTAGAAGATCCGGTCAC+AGG 0.558565 1_2:+8536836 None:intergenic
ATTGTGTCCAATCCTGTGAC+CGG 0.560669 1_2:-8536848 Msa0053990:CDS
CATTCATGAGACATTGCTGC+TGG 0.561199 1_2:+8536248 None:intergenic
AAACCACAAGTCTTAGGGTG+TGG 0.562665 1_2:-8536316 Msa0053990:CDS
ATTGCAAGCTACAACGGTCC+CGG 0.576687 1_2:+8536457 None:intergenic
AGGCGAAAGAAACTCGAAGT+AGG 0.584846 1_2:+8537130 None:intergenic
CTTGCTCACACATCCCCACA+AGG 0.585521 1_2:-8537051 Msa0053990:CDS
TGAATGGTACAGAAAGAAGC+CGG 0.596645 1_2:-8536231 Msa0053990:CDS
TCTGAGCAAGGCTGGAAATG+TGG 0.601419 1_2:-8536573 Msa0053990:CDS
AAGCCGGATATCAATTGTGT+TGG 0.603699 1_2:-8536215 Msa0053990:CDS
AAAACCAAGATATCCGGCTA+GGG 0.604515 1_2:+8536010 None:intergenic
CCGAGTCAAAGATGACAGAC+TGG 0.609122 1_2:+8536993 None:intergenic
AGAAGTCATGGAGTTTATGT+CGG 0.612236 1_2:-8536081 Msa0053990:CDS
AAAGTACACAACATTGTCAT+CGG 0.617043 1_2:+8536595 None:intergenic
TCTCCACACCCTAAGACTTG+TGG 0.621668 1_2:+8536313 None:intergenic
TACTAACAAAGACGGTCAAG+AGG 0.624609 1_2:-8536501 Msa0053990:CDS
TGTTGTGTACTTTCTGAGCA+AGG 0.626293 1_2:-8536585 Msa0053990:CDS
TCCAACCAAGCAATATTCGT+CGG 0.630854 1_2:-8536528 Msa0053990:CDS
GGTCAAATTGCAAGCTACAA+CGG 0.643110 1_2:+8536451 None:intergenic
AAGACGAGAATGCTCTTGGG+AGG 0.645387 1_2:-8536652 Msa0053990:CDS
AAAACCAAGGATCCCTTGTG+GGG 0.654526 1_2:+8537038 None:intergenic
AATACCACTAAATGAGAACA+AGG 0.660886 1_2:+8535787 None:intergenic
TGATTTGGTAACCAACAACT+GGG 0.681708 1_2:-8536132 Msa0053990:CDS
TCGGTCATTACTAACAAAGA+CGG 0.685648 1_2:-8536509 Msa0053990:CDS
TTGCTCACACATCCCCACAA+GGG 0.708158 1_2:-8537050 Msa0053990:CDS
TCTGAGGCGAAATTCAACGG+AGG 0.746256 1_2:+8536809 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATAACAAAAAAAATTAA+AGG + chr1_2:8536006-8536025 None:intergenic 10.0%
!!! ATTTTTATACTGAAATTAAA+AGG - chr1_2:8536044-8536063 Msa0053990:CDS 10.0%
!!! TTTTTATACTGAAATTAAAA+GGG - chr1_2:8536045-8536064 Msa0053990:CDS 10.0%
!!! TTGATAACAAAAAAAATTAA+AGG + chr1_2:8536006-8536025 None:intergenic 10.0%
!!! ATTTTTATACTGAAATTAAA+AGG - chr1_2:8536044-8536063 Msa0053990:CDS 10.0%
!!! TTTTTATACTGAAATTAAAA+GGG - chr1_2:8536045-8536064 Msa0053990:CDS 10.0%
!!! TTTTATACTGAAATTAAAAG+GGG - chr1_2:8536046-8536065 Msa0053990:CDS 15.0%
!!! AAAATTGATTTTTTTGTTGT+TGG - chr1_2:8536174-8536193 Msa0053990:CDS 15.0%
!!! TTTTATACTGAAATTAAAAG+GGG - chr1_2:8536046-8536065 Msa0053990:CDS 15.0%
!!! AAAATTGATTTTTTTGTTGT+TGG - chr1_2:8536174-8536193 Msa0053990:CDS 15.0%
!! CATTAACATATCATGAAAAA+GGG + chr1_2:8536114-8536133 None:intergenic 20.0%
!! ACATTAACATATCATGAAAA+AGG + chr1_2:8536115-8536134 None:intergenic 20.0%
!! CATTAACATATCATGAAAAA+GGG + chr1_2:8536114-8536133 None:intergenic 20.0%
!! ACATTAACATATCATGAAAA+AGG + chr1_2:8536115-8536134 None:intergenic 20.0%
!!! TTTTTACTAGTTCTTGTTTG+AGG - chr1_2:8535849-8535868 Msa0053990:three_prime_UTR 25.0%
!!! TTTTACTAGTTCTTGTTTGA+GGG - chr1_2:8535850-8535869 Msa0053990:three_prime_UTR 25.0%
!!! GAAAGTTTTGAACTTTTTCA+TGG - chr1_2:8536084-8536103 Msa0053990:CDS 25.0%
!! TGTTCTAAATTTCTGTTCAA+TGG - chr1_2:8536233-8536252 Msa0053990:CDS 25.0%
!! ATGAGTACTACATCTTTTAA+TGG - chr1_2:8536723-8536742 Msa0053990:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_2:8536749-8536768 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_2:8536756-8536775 Msa0053990:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_2:8536789-8536808 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_2:8536790-8536809 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_2:8537145-8537164 Msa0053990:CDS 25.0%
! GCTAACAGAACATTAAAAAA+TGG + chr1_2:8537548-8537567 None:intergenic 25.0%
!!! TTTTTACTAGTTCTTGTTTG+AGG - chr1_2:8535849-8535868 Msa0053990:three_prime_UTR 25.0%
!!! TTTTACTAGTTCTTGTTTGA+GGG - chr1_2:8535850-8535869 Msa0053990:three_prime_UTR 25.0%
!!! GAAAGTTTTGAACTTTTTCA+TGG - chr1_2:8536084-8536103 Msa0053990:CDS 25.0%
!! TGTTCTAAATTTCTGTTCAA+TGG - chr1_2:8536233-8536252 Msa0053990:CDS 25.0%
!! ATGAGTACTACATCTTTTAA+TGG - chr1_2:8536723-8536742 Msa0053990:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_2:8536749-8536768 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_2:8536756-8536775 Msa0053990:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_2:8536789-8536808 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_2:8536790-8536809 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_2:8537145-8537164 Msa0053990:CDS 25.0%
! GCTAACAGAACATTAAAAAA+TGG + chr1_2:8537548-8537567 None:intergenic 25.0%
TTTGAGGGTTTGTAGTAAAA+GGG - chr1_2:8535865-8535884 Msa0053990:three_prime_UTR 30.0%
TTGATTACTGTTTGCAATTG+AGG - chr1_2:8535896-8535915 Msa0053990:three_prime_UTR 30.0%
AATAGCTTCTTAAGCTAAGA+GGG - chr1_2:8535953-8535972 Msa0053990:three_prime_UTR 30.0%
ATACTGAAATTAAAAGGGGT+TGG - chr1_2:8536050-8536069 Msa0053990:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_2:8536317-8536336 Msa0053990:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_2:8536694-8536713 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_2:8536757-8536776 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_2:8536892-8536911 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_2:8537092-8537111 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_2:8537337-8537356 Msa0053990:intron 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_2:8537380-8537399 Msa0053990:intron 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_2:8537386-8537405 None:intergenic 30.0%
ATAACCAAAACCAAGATATC+CGG + chr1_2:8537483-8537502 None:intergenic 30.0%
CTGTCAACAATCAAATCATT+TGG + chr1_2:8537639-8537658 None:intergenic 30.0%
TTTGAGGGTTTGTAGTAAAA+GGG - chr1_2:8535865-8535884 Msa0053990:three_prime_UTR 30.0%
TTGATTACTGTTTGCAATTG+AGG - chr1_2:8535896-8535915 Msa0053990:three_prime_UTR 30.0%
AATAGCTTCTTAAGCTAAGA+GGG - chr1_2:8535953-8535972 Msa0053990:three_prime_UTR 30.0%
ATACTGAAATTAAAAGGGGT+TGG - chr1_2:8536050-8536069 Msa0053990:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_2:8536317-8536336 Msa0053990:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_2:8536694-8536713 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_2:8536757-8536776 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_2:8536892-8536911 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_2:8537092-8537111 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_2:8537337-8537356 Msa0053990:intron 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_2:8537380-8537399 Msa0053990:intron 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_2:8537386-8537405 None:intergenic 30.0%
ATAACCAAAACCAAGATATC+CGG + chr1_2:8537483-8537502 None:intergenic 30.0%
CTGTCAACAATCAAATCATT+TGG + chr1_2:8537639-8537658 None:intergenic 30.0%
GTTTGAGGGTTTGTAGTAAA+AGG - chr1_2:8535864-8535883 Msa0053990:three_prime_UTR 35.0%
TTGAGGGTTTGTAGTAAAAG+GGG - chr1_2:8535866-8535885 Msa0053990:three_prime_UTR 35.0%
GAATAGCTTCTTAAGCTAAG+AGG - chr1_2:8535952-8535971 Msa0053990:three_prime_UTR 35.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_2:8536267-8536286 Msa0053990:CDS 35.0%
AAACTACTGAATCCATTCTC+TGG + chr1_2:8536282-8536301 None:intergenic 35.0%
! TGGATTCAGTAGTTTTTCAC+TGG - chr1_2:8536287-8536306 Msa0053990:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_2:8536328-8536347 Msa0053990:CDS 35.0%
! ATCTTTTGCACACAGAAGTA+AGG + chr1_2:8536377-8536396 None:intergenic 35.0%
TTACTTCTGTGTGCAAAAGA+TGG - chr1_2:8536376-8536395 Msa0053990:CDS 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_2:8536568-8536587 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_2:8536681-8536700 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_2:8536808-8536827 Msa0053990:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_2:8536809-8536828 Msa0053990:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_2:8536975-8536994 Msa0053990:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_2:8537008-8537027 Msa0053990:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_2:8537009-8537028 Msa0053990:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_2:8537079-8537098 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_2:8537110-8537129 Msa0053990:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_2:8537297-8537316 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_2:8537352-8537371 Msa0053990:intron 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_2:8537391-8537410 Msa0053990:intron 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_2:8537403-8537422 Msa0053990:intron 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_2:8537443-8537462 None:intergenic 35.0%
GAATAGAAGCTTCTGTTTGA+CGG - chr1_2:8537444-8537463 Msa0053990:intron 35.0%
TCATGCAACATAGCTAGATA+TGG - chr1_2:8537606-8537625 Msa0053990:intron 35.0%
GTAACCTTGTTCTCATTTAG+TGG - chr1_2:8537693-8537712 Msa0053990:intron 35.0%
GTTTGAGGGTTTGTAGTAAA+AGG - chr1_2:8535864-8535883 Msa0053990:three_prime_UTR 35.0%
TTGAGGGTTTGTAGTAAAAG+GGG - chr1_2:8535866-8535885 Msa0053990:three_prime_UTR 35.0%
GAATAGCTTCTTAAGCTAAG+AGG - chr1_2:8535952-8535971 Msa0053990:three_prime_UTR 35.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_2:8536267-8536286 Msa0053990:CDS 35.0%
AAACTACTGAATCCATTCTC+TGG + chr1_2:8536282-8536301 None:intergenic 35.0%
! TGGATTCAGTAGTTTTTCAC+TGG - chr1_2:8536287-8536306 Msa0053990:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_2:8536328-8536347 Msa0053990:CDS 35.0%
! ATCTTTTGCACACAGAAGTA+AGG + chr1_2:8536377-8536396 None:intergenic 35.0%
TTACTTCTGTGTGCAAAAGA+TGG - chr1_2:8536376-8536395 Msa0053990:CDS 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_2:8536568-8536587 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_2:8536681-8536700 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_2:8536808-8536827 Msa0053990:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_2:8536809-8536828 Msa0053990:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_2:8536975-8536994 Msa0053990:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_2:8537008-8537027 Msa0053990:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_2:8537009-8537028 Msa0053990:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_2:8537079-8537098 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_2:8537110-8537129 Msa0053990:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_2:8537297-8537316 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_2:8537352-8537371 Msa0053990:intron 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_2:8537391-8537410 Msa0053990:intron 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_2:8537403-8537422 Msa0053990:intron 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_2:8537443-8537462 None:intergenic 35.0%
GAATAGAAGCTTCTGTTTGA+CGG - chr1_2:8537444-8537463 Msa0053990:intron 35.0%
TCATGCAACATAGCTAGATA+TGG - chr1_2:8537606-8537625 Msa0053990:intron 35.0%
TGTTTGCAATTGAGGTCTCA+AGG - chr1_2:8535904-8535923 Msa0053990:three_prime_UTR 40.0%
TAAAAGGGGTTGGAGAATGA+AGG - chr1_2:8536060-8536079 Msa0053990:CDS 40.0%
AAAAGGGGTTGGAGAATGAA+GGG - chr1_2:8536061-8536080 Msa0053990:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_2:8536450-8536469 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_2:8536452-8536471 Msa0053990:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_2:8536546-8536565 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_2:8536579-8536598 Msa0053990:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_2:8536617-8536636 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_2:8536670-8536689 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_2:8536899-8536918 Msa0053990:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_2:8536956-8536975 Msa0053990:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_2:8536964-8536983 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_2:8536983-8537002 Msa0053990:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_2:8537036-8537055 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_2:8537057-8537076 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_2:8537060-8537079 Msa0053990:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_2:8537080-8537099 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_2:8537162-8537181 Msa0053990:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_2:8537163-8537182 Msa0053990:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_2:8537253-8537272 Msa0053990:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_2:8537269-8537288 Msa0053990:intron 40.0%
GCACCAACACAATTGATATC+CGG + chr1_2:8537275-8537294 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_2:8537275-8537294 Msa0053990:intron 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_2:8537329-8537348 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_2:8537351-8537370 Msa0053990:intron 40.0%
AAAACCAAGATATCCGGCTA+GGG + chr1_2:8537477-8537496 None:intergenic 40.0%
TGTTTGCAATTGAGGTCTCA+AGG - chr1_2:8535904-8535923 Msa0053990:three_prime_UTR 40.0%
TAAAAGGGGTTGGAGAATGA+AGG - chr1_2:8536060-8536079 Msa0053990:CDS 40.0%
AAAAGGGGTTGGAGAATGAA+GGG - chr1_2:8536061-8536080 Msa0053990:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_2:8536450-8536469 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_2:8536452-8536471 Msa0053990:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_2:8536546-8536565 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_2:8536579-8536598 Msa0053990:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_2:8536617-8536636 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_2:8536670-8536689 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_2:8536899-8536918 Msa0053990:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_2:8536956-8536975 Msa0053990:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_2:8536964-8536983 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_2:8536983-8537002 Msa0053990:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_2:8537036-8537055 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_2:8537057-8537076 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_2:8537060-8537079 Msa0053990:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_2:8537080-8537099 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_2:8537162-8537181 Msa0053990:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_2:8537163-8537182 Msa0053990:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_2:8537253-8537272 Msa0053990:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_2:8537269-8537288 Msa0053990:intron 40.0%
GCACCAACACAATTGATATC+CGG + chr1_2:8537275-8537294 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_2:8537275-8537294 Msa0053990:intron 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_2:8537329-8537348 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_2:8537351-8537370 Msa0053990:intron 40.0%
AAAACCAAGATATCCGGCTA+GGG + chr1_2:8537477-8537496 None:intergenic 40.0%
AAAGGGGTTGGAGAATGAAG+GGG - chr1_2:8536062-8536081 Msa0053990:CDS 45.0%
AGGCGAAAGAAACTCGAAGT+AGG + chr1_2:8536357-8536376 None:intergenic 45.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_2:8536449-8536468 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_2:8536451-8536470 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_2:8536462-8536481 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_2:8536483-8536502 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_2:8536484-8536503 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_2:8536491-8536510 Msa0053990:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_2:8536505-8536524 Msa0053990:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_2:8536636-8536655 Msa0053990:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_2:8536658-8536677 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_2:8536960-8536979 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_2:8537168-8537187 Msa0053990:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_2:8537239-8537258 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_2:8537237-8537256 Msa0053990:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_2:8537366-8537385 None:intergenic 45.0%
CAAAACCAAGATATCCGGCT+AGG + chr1_2:8537478-8537497 None:intergenic 45.0%
! CTAGCCGGATATCTTGGTTT+TGG - chr1_2:8537476-8537495 Msa0053990:intron 45.0%
AAAGGGGTTGGAGAATGAAG+GGG - chr1_2:8536062-8536081 Msa0053990:CDS 45.0%
AGGCGAAAGAAACTCGAAGT+AGG + chr1_2:8536357-8536376 None:intergenic 45.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_2:8536449-8536468 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_2:8536451-8536470 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_2:8536462-8536481 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_2:8536483-8536502 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_2:8536484-8536503 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_2:8536491-8536510 Msa0053990:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_2:8536505-8536524 Msa0053990:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_2:8536636-8536655 Msa0053990:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_2:8536658-8536677 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_2:8536960-8536979 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_2:8537168-8537187 Msa0053990:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_2:8537239-8537258 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_2:8537237-8537256 Msa0053990:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_2:8537366-8537385 None:intergenic 45.0%
CAAAACCAAGATATCCGGCT+AGG + chr1_2:8537478-8537497 None:intergenic 45.0%
! CTAGCCGGATATCTTGGTTT+TGG - chr1_2:8537476-8537495 Msa0053990:intron 45.0%
!! AAAAAAAATAACATAAAAAA+TGG + chr1_2:8537509-8537528 None:intergenic 5.0%
!! AAAAAAAATAACATAAAAAA+TGG + chr1_2:8537509-8537528 None:intergenic 5.0%
TTGCTCACACATCCCCACAA+GGG - chr1_2:8536434-8536453 Msa0053990:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_2:8536494-8536513 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_2:8536585-8536604 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_2:8536584-8536603 Msa0053990:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_2:8536605-8536624 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_2:8536606-8536625 Msa0053990:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_2:8536646-8536665 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_2:8536678-8536697 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_2:8536828-8536847 Msa0053990:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_2:8536829-8536848 Msa0053990:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_2:8536832-8536851 Msa0053990:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_2:8536903-8536922 Msa0053990:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_2:8536911-8536930 Msa0053990:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_2:8537030-8537049 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_2:8537174-8537193 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_2:8537207-8537226 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_2:8537211-8537230 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_2:8537282-8537301 Msa0053990:intron 50.0%
AATGCCCTAGCCGGATATCT+TGG - chr1_2:8537470-8537489 Msa0053990:intron 50.0%
TTGCTCACACATCCCCACAA+GGG - chr1_2:8536434-8536453 Msa0053990:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_2:8536494-8536513 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_2:8536585-8536604 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_2:8536584-8536603 Msa0053990:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_2:8536605-8536624 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_2:8536606-8536625 Msa0053990:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_2:8536646-8536665 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_2:8536678-8536697 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_2:8536828-8536847 Msa0053990:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_2:8536829-8536848 Msa0053990:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_2:8536832-8536851 Msa0053990:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_2:8536903-8536922 Msa0053990:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_2:8536911-8536930 Msa0053990:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_2:8537030-8537049 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_2:8537174-8537193 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_2:8537207-8537226 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_2:8537211-8537230 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_2:8537282-8537301 Msa0053990:intron 50.0%
AATGCCCTAGCCGGATATCT+TGG - chr1_2:8537470-8537489 Msa0053990:intron 50.0%
CTTGCTCACACATCCCCACA+AGG - chr1_2:8536433-8536452 Msa0053990:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_2:8536442-8536461 Msa0053990:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_2:8536651-8536670 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_2:8536836-8536855 Msa0053990:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_2:8537195-8537214 Msa0053990:CDS 55.0%
TGACGGTGAAATGCCCTAGC+CGG - chr1_2:8537461-8537480 Msa0053990:intron 55.0%
CTTGCTCACACATCCCCACA+AGG - chr1_2:8536433-8536452 Msa0053990:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_2:8536442-8536461 Msa0053990:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_2:8536651-8536670 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_2:8536836-8536855 Msa0053990:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_2:8537195-8537214 Msa0053990:CDS 55.0%
TGACGGTGAAATGCCCTAGC+CGG - chr1_2:8537461-8537480 Msa0053990:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 8535791 8537715 8535791 ID=Msa0053990;Name=Msa0053990
chr1_2 mRNA 8535791 8537715 8535791 ID=Msa0053990-mRNA-1;Parent=Msa0053990;Name=Msa0053990-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0.33|0.33|1|1|3|252|415
chr1_2 exon 8535791 8537278 8535791 ID=Msa0053990-mRNA-1:exon:3067;Parent=Msa0053990-mRNA-1
chr1_2 exon 8537581 8537588 8537581 ID=Msa0053990-mRNA-1:exon:3066;Parent=Msa0053990-mRNA-1
chr1_2 exon 8537712 8537715 8537712 ID=Msa0053990-mRNA-1:exon:3065;Parent=Msa0053990-mRNA-1
chr1_2 CDS 8537712 8537715 8537712 ID=Msa0053990-mRNA-1:cds;Parent=Msa0053990-mRNA-1
chr1_2 CDS 8537581 8537588 8537581 ID=Msa0053990-mRNA-1:cds;Parent=Msa0053990-mRNA-1
chr1_2 CDS 8536043 8537278 8536043 ID=Msa0053990-mRNA-1:cds;Parent=Msa0053990-mRNA-1
chr1_2 three_prime_UTR 8535791 8536042 8535791 ID=Msa0053990-mRNA-1:three_prime_utr;Parent=Msa0053990-mRNA-1
Gene Sequence

>Msa0053990

ATGTGTCTCAAGTTGTTTGTTCTAAATTTCTGTTCAATGGAACATAGATTGAAAAGAAAGTCTCCAGAGAATGGATTCAGTAGTTTTTCACTGGATGATCTGAATGAAGATGTCTTTGAAAAGGTACTTTCGTGGTTACCTACTTCGAGTTTCTTTCGCCTTACTTCTGTGTGCAAAAGATGGAAATCAGCTGCTGATTCTGCGAGTTTCAAGCTCGCTTGCTCACACATCCCCACAAGGGATCCTTGGTTTTTCATGGTTGCTCCCGATCTCAACCAGTCTGTCATCTTTGACTCGGCTGAAAACAGTTGGAAAAGACTCAATCATCCGAATCTTCTGCTCGAAGATTCCAACAAAAGTTGCATTCCTGTTGCAGCTTCTAATGGCCTGGTTTGCTACCGAACTTCGTCAGGAAACTTCATTGTGTCCAATCCTGTGACCGGATCTTCTACCGAACTACCTCCGTTGAATTTCGCCTCAGAAAATCAATCTCTCAATGCTGTTGTTATGAGTACTACATCTTTTAATGGCCAAATATCCTACAAGATTGTATTAGTCTTCGGTGAACTTCCCAATCTTTCGTTTAAAGTCTATAACTCAAGCTCTTCTTGTTGGGAAGACGAGAATGCTCTTGGGAGGAAGGTCGATGATAGTTCAGCTGATTGCGATTCAACCGATGACAATGTTGTGTACTTTCTGAGCAAGGCTGGAAATGTGGTAGCAAGTAGCATGCAGAGAAGTCCAACCAAGCAATATTCGTCGGTCATTACTAACAAAGACGGTCAAGAGGTAGTCTATTTTTTGAGTTCGTCCGGGACCGTTGTAGCTTGCAATTTGACCTGCAAGTGTTTCTTTGAGTACCCTAGGTTGTTGCCAGTTTTTTGCGAATACTCTATTGATATCGTCGAGTGTAACGGTGAGATGTTAGTCGTTTTATTATCAGAATTCTTGGAAACCACAAGTCTTAGGGTGTGGAGATTCGATGAGACCAACCGATGCTGGCAACAGATTGCAGCAATGCCAGCAGCAATGTCTCATGAATGGTACAGAAAGAAGCCGGATATCAATTGTGTTGGTGCCGGTTCTCGGATTTTTATATGCTTGAACTCTCCTGAGCTATGCACTTATGTTCTTTGTGATTTGGTAACCAACAACTGGGTTGAATTGCCAAAATGTTGCATTAATGGAGAAGTCATGGAGTTTATGTCGGCATTTTCTTTCGAGCCAAGAATAGAAGCTTCTGTTTGA
Protein sequence

>Msa0053990

MCLKLFVLNFCSMEHRLKRKSPENGFSSFSLDDLNEDVFEKVLSWLPTSSFFRLTSVCKRWKSAADSASFKLACSHIPTRDPWFFMVAPDLNQSVIFDSAENSWKRLNHPNLLLEDSNKSCIPVAASNGLVCYRTSSGNFIVSNPVTGSSTELPPLNFASENQSLNAVVMSTTSFNGQISYKIVLVFGELPNLSFKVYNSSSSCWEDENALGRKVDDSSADCDSTDDNVVYFLSKAGNVVASSMQRSPTKQYSSVITNKDGQEVVYFLSSSGTVVACNLTCKCFFEYPRLLPVFCEYSIDIVECNGEMLVVLLSEFLETTSLRVWRFDETNRCWQQIAAMPAAMSHEWYRKKPDINCVGAGSRIFICLNSPELCTYVLCDLVTNNWVELPKCCINGEVMEFMSAFSFEPRIEASV*