AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0096560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096560 MtrunA17_Chr1g0150561 98.015 403 8 0 1 403 1 403 0.0 811
Msa0096560 MtrunA17_Chr3g0122401 25.258 388 241 13 21 401 118 463 3.08e-30 121
Msa0096560 MtrunA17_Chr8g0347881 25.304 411 236 18 17 402 39 403 2.05e-20 92.4
Msa0096560 MtrunA17_Chr1g0161891 22.192 365 221 11 20 378 19 326 4.09e-18 85.1
Msa0096560 MtrunA17_Chr8g0347851 25.441 397 238 19 22 402 44 398 3.55e-13 70.5
Msa0096560 MtrunA17_Chr1g0161871 26.201 229 137 8 20 230 30 244 2.16e-12 68.2
Msa0096560 MtrunA17_Chr7g0220421 22.506 391 243 12 22 403 98 437 2.61e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0096560 AT4G33160.1 57.179 397 156 12 17 403 65 457 8.41e-146 422
Msa0096560 AT4G33160.2 57.179 397 156 12 17 403 126 518 3.19e-145 423
Msa0096560 AT1G27340.1 24.528 371 228 12 20 382 118 444 1.23e-26 111
Msa0096560 AT3G61590.4 24.941 421 246 22 11 402 67 446 4.08e-12 67.8
Msa0096560 AT3G61590.1 24.941 421 246 22 11 402 31 410 5.35e-12 67.4
Msa0096560 AT3G61590.2 24.941 421 246 22 11 402 39 418 5.85e-12 67.4
Msa0096560 AT3G61590.3 24.941 421 246 22 11 402 39 418 5.85e-12 67.4

Find 78 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAAAATGTTGCATTAA+TGG 0.164880 1_3:-2244544 Msa0096560:CDS
ATACAATCTTGTAGGATATT+TGG 0.194528 1_3:+2245178 None:intergenic
CAAACAGAAGCTTCTATTCT+TGG 0.227716 1_3:+2244484 None:intergenic
TCTTGGAAACCACAAGTCTT+AGG 0.228546 1_3:-2244762 Msa0096560:CDS
TCTCCATTAATGCAACATTT+TGG 0.236954 1_3:+2244541 None:intergenic
CACTTATGTTCTTTGTGATT+TGG 0.296690 1_3:-2244587 Msa0096560:CDS
ATTCCTGTTGCAGCTTCTAA+TGG 0.302810 1_3:-2245345 Msa0096560:CDS
ATTGTGTTGGTGCCGGTTCT+CGG 0.340920 1_3:-2244642 Msa0096560:CDS
GTGATTTGGTAACCAACAAC+TGG 0.347069 1_3:-2244573 Msa0096560:CDS
ATAGACTTTAAACGAAAGAT+TGG 0.375910 1_3:+2245137 None:intergenic
GAAGACTAATACAATCTTGT+AGG 0.389829 1_3:+2245170 None:intergenic
ATAACTCAAGCTCTTCTTGT+TGG 0.420790 1_3:-2245116 Msa0096560:CDS
TGTTGCAGCTTCTAATGGCC+TGG 0.422631 1_3:-2245340 Msa0096560:CDS
GAATAGAAGCTTCTGTTTGA+CGG 0.426490 1_3:-2244480 None:intergenic
TAACTCAAGCTCTTCTTGTT+GGG 0.426988 1_3:-2245115 Msa0096560:CDS
TACAAGATTGTATTAGTCTT+CGG 0.428289 1_3:-2245168 Msa0096560:CDS
GAAATTCAACGGAGGTAGTT+CGG 0.429403 1_3:+2245257 None:intergenic
AAACTATTGAATCCATTCTC+TGG 0.430777 1_3:+2245645 None:intergenic
CTTGGAAACCACAAGTCTTA+GGG 0.431925 1_3:-2244761 Msa0096560:CDS
TGAAGTTTCCTGACGAAGTT+CGG 0.432189 1_3:+2245310 None:intergenic
TGGGAAGACGAGAATGCTCT+TGG 0.432787 1_3:-2245096 Msa0096560:CDS
GTTTGCTACCGAACTTCGTC+AGG 0.434074 1_3:-2245318 Msa0096560:CDS
AGTGTTTCTTTGAGTACCCT+AGG 0.452698 1_3:-2244864 Msa0096560:CDS
AAAAGAAAGTCTCCAGAGAA+TGG 0.455501 1_3:-2245657 Msa0096560:CDS
ACATCCCCACAAGGGATCCT+TGG 0.462778 1_3:-2245482 Msa0096560:CDS
TGGCAATTCAACCCAGTTGT+TGG 0.463420 1_3:+2244561 None:intergenic
AGAAGATCCGGTCACAGGAT+TGG 0.466882 1_3:+2245281 None:intergenic
GAATCTTCGAGCAGAAGATT+CGG 0.467832 1_3:+2245381 None:intergenic
TAGACTTTAAACGAAAGATT+GGG 0.470551 1_3:+2245138 None:intergenic
TCGATGAGACCAACCGATGC+TGG 0.476739 1_3:-2244729 Msa0096560:CDS
TTACTTCCGTGTGCAAAAGA+TGG 0.481747 1_3:-2245548 Msa0096560:CDS
ATGACAGACTGGTTGAGATC+GGG 0.482940 1_3:+2245444 None:intergenic
GATATCAATTGTGTTGGTGC+CGG 0.486697 1_3:-2244649 Msa0096560:CDS
GTACTCAAAGAAACACTTGC+AGG 0.487063 1_3:+2244870 None:intergenic
TTGACTCGGCTGAAAACAGT+TGG 0.492513 1_3:-2245419 Msa0096560:CDS
GAATGAAGATGTCTTTGAAA+AGG 0.497404 1_3:-2245607 Msa0096560:CDS
GATGACAGACTGGTTGAGAT+CGG 0.499005 1_3:+2245443 None:intergenic
AGGCCATTAGAAGCTGCAAC+AGG 0.501616 1_3:+2245342 None:intergenic
GCACCAACACAATTGATATC+CGG 0.505766 1_3:+2244652 None:intergenic
AGGTAGTTCGGTAGAAGATC+CGG 0.510241 1_3:+2245269 None:intergenic
CGAGAATGCTCTTGGGAGGA+AGG 0.510339 1_3:-2245088 Msa0096560:CDS
ACCGACGAATATTGCTTGGT+TGG 0.512080 1_3:+2244967 None:intergenic
AATGACCGACGAATATTGCT+TGG 0.513209 1_3:+2244963 None:intergenic
GTGTACTTTCTGAGCAAGGC+TGG 0.518608 1_3:-2245021 Msa0096560:CDS
ATTGATATCGTCGAGTGTAA+CGG 0.526717 1_3:-2244814 Msa0096560:CDS
TCTTTGAAAAGGTACTTTCG+TGG 0.527524 1_3:-2245596 Msa0096560:CDS
ACGAAGTTCGGTAGCAAACC+AGG 0.530457 1_3:+2245322 None:intergenic
CAGCAGCAATGTCTCATGAA+TGG 0.530978 1_3:-2244687 Msa0096560:CDS
AGAACATAAGTGCATAGCTC+AGG 0.532205 1_3:+2244598 None:intergenic
GGGAAGACGAGAATGCTCTT+GGG 0.541458 1_3:-2245095 Msa0096560:CDS
CTGCAATCTGTTGCCAGCAT+CGG 0.543898 1_3:+2244716 None:intergenic
TTGCATTAATGGAGAAGTCA+TGG 0.546480 1_3:-2244533 Msa0096560:CDS
AATCTGTTGCCAGCATCGGT+TGG 0.557369 1_3:+2244720 None:intergenic
CCAGTCTGTCATCTTTGACT+CGG 0.557680 1_3:-2245433 Msa0096560:CDS
TCGGTAGAAGATCCGGTCAC+AGG 0.558565 1_3:+2245276 None:intergenic
ATTGTGTCCAATCCTGTGAC+CGG 0.560669 1_3:-2245288 Msa0096560:CDS
CATTCATGAGACATTGCTGC+TGG 0.561199 1_3:+2244688 None:intergenic
AAACCACAAGTCTTAGGGTG+TGG 0.562665 1_3:-2244756 Msa0096560:CDS
ATTGCAAGCTACAACGGTCC+CGG 0.576687 1_3:+2244897 None:intergenic
CTTGCTCACACATCCCCACA+AGG 0.585521 1_3:-2245491 Msa0096560:CDS
AGACGAAAGAAACTCGAAGT+AGG 0.593990 1_3:+2245570 None:intergenic
TGAATGGTACAGAAAGAAGC+CGG 0.596645 1_3:-2244671 Msa0096560:CDS
TCTGAGCAAGGCTGGAAATG+TGG 0.601419 1_3:-2245013 Msa0096560:CDS
AAGCCGGATATCAATTGTGT+TGG 0.603699 1_3:-2244655 Msa0096560:CDS
CCGAGTCAAAGATGACAGAC+TGG 0.609122 1_3:+2245433 None:intergenic
AGAAGTCATGGAGTTTATGT+CGG 0.612236 1_3:-2244521 Msa0096560:CDS
AAAGTACACAACATTGTCAT+CGG 0.617043 1_3:+2245035 None:intergenic
TCTCCACACCCTAAGACTTG+TGG 0.621668 1_3:+2244753 None:intergenic
TACTAACAAAGACGGTCAAG+AGG 0.624609 1_3:-2244941 Msa0096560:CDS
TGTTGTGTACTTTCTGAGCA+AGG 0.626293 1_3:-2245025 Msa0096560:CDS
TCCAACCAAGCAATATTCGT+CGG 0.630854 1_3:-2244968 Msa0096560:CDS
GGTCAAATTGCAAGCTACAA+CGG 0.643110 1_3:+2244891 None:intergenic
AAGACGAGAATGCTCTTGGG+AGG 0.645387 1_3:-2245092 Msa0096560:CDS
AAAACCAAGGATCCCTTGTG+GGG 0.654526 1_3:+2245478 None:intergenic
TGATTTGGTAACCAACAACT+GGG 0.681708 1_3:-2244572 Msa0096560:CDS
TCGGTCATTACTAACAAAGA+CGG 0.685648 1_3:-2244949 Msa0096560:CDS
TTGCTCACACATCCCCACAA+GGG 0.708158 1_3:-2245490 Msa0096560:CDS
TCTGAGGCGAAATTCAACGG+AGG 0.746256 1_3:+2245249 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAGTACTACATCTTTTAA+TGG - chr1_3:2244954-2244973 Msa0096560:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_3:2244980-2244999 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_3:2244987-2245006 Msa0096560:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_3:2245020-2245039 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_3:2245021-2245040 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_3:2245376-2245395 Msa0096560:CDS 25.0%
!! ATGAGTACTACATCTTTTAA+TGG - chr1_3:2244954-2244973 Msa0096560:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_3:2244980-2244999 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_3:2244987-2245006 Msa0096560:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_3:2245020-2245039 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_3:2245021-2245040 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_3:2245376-2245395 Msa0096560:CDS 25.0%
AAACTATTGAATCCATTCTC+TGG + chr1_3:2244513-2244532 None:intergenic 30.0%
! TGGATTCAATAGTTTTTCAC+TGG - chr1_3:2244518-2244537 Msa0096560:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_3:2244548-2244567 Msa0096560:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_3:2244925-2244944 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_3:2244988-2245007 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_3:2245123-2245142 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_3:2245323-2245342 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_3:2245568-2245587 Msa0096560:CDS 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_3:2245611-2245630 Msa0096560:CDS 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_3:2245617-2245636 None:intergenic 30.0%
AAACTATTGAATCCATTCTC+TGG + chr1_3:2244513-2244532 None:intergenic 30.0%
! TGGATTCAATAGTTTTTCAC+TGG - chr1_3:2244518-2244537 Msa0096560:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_3:2244548-2244567 Msa0096560:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_3:2244925-2244944 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_3:2244988-2245007 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_3:2245123-2245142 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_3:2245323-2245342 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_3:2245568-2245587 Msa0096560:CDS 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_3:2245611-2245630 Msa0096560:CDS 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_3:2245617-2245636 None:intergenic 30.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_3:2244498-2244517 Msa0096560:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_3:2244559-2244578 Msa0096560:CDS 35.0%
! TGATTTCCATCTTTTGCACA+CGG + chr1_3:2244616-2244635 None:intergenic 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_3:2244799-2244818 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_3:2244912-2244931 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_3:2245039-2245058 Msa0096560:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_3:2245040-2245059 Msa0096560:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_3:2245206-2245225 Msa0096560:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_3:2245239-2245258 Msa0096560:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_3:2245240-2245259 Msa0096560:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_3:2245310-2245329 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_3:2245341-2245360 Msa0096560:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_3:2245528-2245547 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_3:2245583-2245602 Msa0096560:CDS 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_3:2245622-2245641 Msa0096560:CDS 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_3:2245634-2245653 Msa0096560:CDS 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_3:2245674-2245693 None:intergenic 35.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_3:2244498-2244517 Msa0096560:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_3:2244559-2244578 Msa0096560:CDS 35.0%
! TGATTTCCATCTTTTGCACA+CGG + chr1_3:2244616-2244635 None:intergenic 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_3:2244799-2244818 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_3:2244912-2244931 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_3:2245039-2245058 Msa0096560:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_3:2245040-2245059 Msa0096560:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_3:2245206-2245225 Msa0096560:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_3:2245239-2245258 Msa0096560:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_3:2245240-2245259 Msa0096560:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_3:2245310-2245329 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_3:2245341-2245360 Msa0096560:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_3:2245528-2245547 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_3:2245583-2245602 Msa0096560:CDS 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_3:2245622-2245641 Msa0096560:CDS 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_3:2245634-2245653 Msa0096560:CDS 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_3:2245674-2245693 None:intergenic 35.0%
AGACGAAAGAAACTCGAAGT+AGG + chr1_3:2244588-2244607 None:intergenic 40.0%
TTACTTCCGTGTGCAAAAGA+TGG - chr1_3:2244607-2244626 Msa0096560:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_3:2244681-2244700 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_3:2244683-2244702 Msa0096560:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_3:2244777-2244796 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_3:2244810-2244829 Msa0096560:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_3:2244848-2244867 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_3:2244901-2244920 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_3:2245130-2245149 Msa0096560:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_3:2245187-2245206 Msa0096560:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_3:2245195-2245214 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_3:2245214-2245233 Msa0096560:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_3:2245267-2245286 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_3:2245288-2245307 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_3:2245291-2245310 Msa0096560:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_3:2245311-2245330 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_3:2245393-2245412 Msa0096560:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_3:2245394-2245413 Msa0096560:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_3:2245484-2245503 Msa0096560:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_3:2245500-2245519 Msa0096560:CDS 40.0%
GCACCAACACAATTGATATC+CGG + chr1_3:2245506-2245525 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_3:2245506-2245525 Msa0096560:CDS 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_3:2245560-2245579 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_3:2245582-2245601 Msa0096560:CDS 40.0%
AGACGAAAGAAACTCGAAGT+AGG + chr1_3:2244588-2244607 None:intergenic 40.0%
TTACTTCCGTGTGCAAAAGA+TGG - chr1_3:2244607-2244626 Msa0096560:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_3:2244681-2244700 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_3:2244683-2244702 Msa0096560:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_3:2244777-2244796 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_3:2244810-2244829 Msa0096560:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_3:2244848-2244867 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_3:2244901-2244920 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_3:2245130-2245149 Msa0096560:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_3:2245187-2245206 Msa0096560:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_3:2245195-2245214 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_3:2245214-2245233 Msa0096560:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_3:2245267-2245286 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_3:2245288-2245307 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_3:2245291-2245310 Msa0096560:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_3:2245311-2245330 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_3:2245393-2245412 Msa0096560:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_3:2245394-2245413 Msa0096560:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_3:2245484-2245503 Msa0096560:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_3:2245500-2245519 Msa0096560:CDS 40.0%
GCACCAACACAATTGATATC+CGG + chr1_3:2245506-2245525 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_3:2245506-2245525 Msa0096560:CDS 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_3:2245560-2245579 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_3:2245582-2245601 Msa0096560:CDS 40.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_3:2244680-2244699 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_3:2244682-2244701 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_3:2244693-2244712 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_3:2244714-2244733 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_3:2244715-2244734 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_3:2244722-2244741 Msa0096560:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_3:2244736-2244755 Msa0096560:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_3:2244867-2244886 Msa0096560:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_3:2244889-2244908 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_3:2245191-2245210 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_3:2245399-2245418 Msa0096560:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_3:2245470-2245489 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_3:2245468-2245487 Msa0096560:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_3:2245597-2245616 None:intergenic 45.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_3:2244680-2244699 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_3:2244682-2244701 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_3:2244693-2244712 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_3:2244714-2244733 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_3:2244715-2244734 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_3:2244722-2244741 Msa0096560:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_3:2244736-2244755 Msa0096560:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_3:2244867-2244886 Msa0096560:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_3:2244889-2244908 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_3:2245191-2245210 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_3:2245399-2245418 Msa0096560:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_3:2245470-2245489 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_3:2245468-2245487 Msa0096560:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_3:2245597-2245616 None:intergenic 45.0%
TTGCTCACACATCCCCACAA+GGG - chr1_3:2244665-2244684 Msa0096560:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_3:2244725-2244744 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_3:2244816-2244835 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_3:2244815-2244834 Msa0096560:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_3:2244836-2244855 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_3:2244837-2244856 Msa0096560:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_3:2244877-2244896 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_3:2244909-2244928 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_3:2245059-2245078 Msa0096560:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_3:2245060-2245079 Msa0096560:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_3:2245063-2245082 Msa0096560:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_3:2245134-2245153 Msa0096560:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_3:2245142-2245161 Msa0096560:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_3:2245261-2245280 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_3:2245405-2245424 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_3:2245438-2245457 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_3:2245442-2245461 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_3:2245513-2245532 Msa0096560:CDS 50.0%
TTGCTCACACATCCCCACAA+GGG - chr1_3:2244665-2244684 Msa0096560:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_3:2244725-2244744 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_3:2244816-2244835 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_3:2244815-2244834 Msa0096560:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_3:2244836-2244855 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_3:2244837-2244856 Msa0096560:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_3:2244877-2244896 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_3:2244909-2244928 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_3:2245059-2245078 Msa0096560:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_3:2245060-2245079 Msa0096560:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_3:2245063-2245082 Msa0096560:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_3:2245134-2245153 Msa0096560:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_3:2245142-2245161 Msa0096560:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_3:2245261-2245280 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_3:2245405-2245424 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_3:2245438-2245457 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_3:2245442-2245461 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_3:2245513-2245532 Msa0096560:CDS 50.0%
CTTGCTCACACATCCCCACA+AGG - chr1_3:2244664-2244683 Msa0096560:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_3:2244673-2244692 Msa0096560:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_3:2244882-2244901 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_3:2245067-2245086 Msa0096560:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_3:2245426-2245445 Msa0096560:CDS 55.0%
CTTGCTCACACATCCCCACA+AGG - chr1_3:2244664-2244683 Msa0096560:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_3:2244673-2244692 Msa0096560:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_3:2244882-2244901 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_3:2245067-2245086 Msa0096560:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_3:2245426-2245445 Msa0096560:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 2244483 2245694 2244483 ID=Msa0096560;Name=Msa0096560
chr1_3 mRNA 2244483 2245694 2244483 ID=Msa0096560-mRNA-1;Parent=Msa0096560;Name=Msa0096560-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|403
chr1_3 exon 2244483 2245694 2244483 ID=Msa0096560-mRNA-1:exon:547;Parent=Msa0096560-mRNA-1
chr1_3 CDS 2244483 2245694 2244483 ID=Msa0096560-mRNA-1:cds;Parent=Msa0096560-mRNA-1
Gene Sequence

>Msa0096560

ATGGAACATAGATTGAAAAGAAAGTCTCCAGAGAATGGATTCAATAGTTTTTCACTGGATGATCTGAATGAAGATGTCTTTGAAAAGGTACTTTCGTGGTTACCTACTTCGAGTTTCTTTCGTCTTACTTCCGTGTGCAAAAGATGGAAATCAGCTGCTGATTCTGCGAGTTTCAAGCTCGCTTGCTCACACATCCCCACAAGGGATCCTTGGTTTTTCATGGTTGCTCCCGATCTCAACCAGTCTGTCATCTTTGACTCGGCTGAAAACAGTTGGAAAAGACTCAATCATCCGAATCTTCTGCTCGAAGATTCCAACAAAAGTTGCATTCCTGTTGCAGCTTCTAATGGCCTGGTTTGCTACCGAACTTCGTCAGGAAACTTCATTGTGTCCAATCCTGTGACCGGATCTTCTACCGAACTACCTCCGTTGAATTTCGCCTCAGAAAATCAATCTCTCAATGCTGTTGTTATGAGTACTACATCTTTTAATGGCCAAATATCCTACAAGATTGTATTAGTCTTCGGTGAACTTCCCAATCTTTCGTTTAAAGTCTATAACTCAAGCTCTTCTTGTTGGGAAGACGAGAATGCTCTTGGGAGGAAGGTCGATGATAGTTCAGCTGATTGCGATTCAACCGATGACAATGTTGTGTACTTTCTGAGCAAGGCTGGAAATGTGGTAGCAAGTAGCATGCAGAGAAGTCCAACCAAGCAATATTCGTCGGTCATTACTAACAAAGACGGTCAAGAGGTAGTCTATTTTTTGAGTTCGTCCGGGACCGTTGTAGCTTGCAATTTGACCTGCAAGTGTTTCTTTGAGTACCCTAGGTTGTTGCCAGTTTTTTGCGAATACTCTATTGATATCGTCGAGTGTAACGGTGAGATGTTAGTCGTTTTATTATCAGAATTCTTGGAAACCACAAGTCTTAGGGTGTGGAGATTCGATGAGACCAACCGATGCTGGCAACAGATTGCAGCAATGCCAGCAGCAATGTCTCATGAATGGTACAGAAAGAAGCCGGATATCAATTGTGTTGGTGCCGGTTCTCGGATTTTTATATGCTTGAACTCTCCTGAGCTATGCACTTATGTTCTTTGTGATTTGGTAACCAACAACTGGGTTGAATTGCCAAAATGTTGCATTAATGGAGAAGTCATGGAGTTTATGTCGGCATTTTCTTTCGAGCCAAGAATAGAAGCTTCTGTTTGA
Protein sequence

>Msa0096560

MEHRLKRKSPENGFNSFSLDDLNEDVFEKVLSWLPTSSFFRLTSVCKRWKSAADSASFKLACSHIPTRDPWFFMVAPDLNQSVIFDSAENSWKRLNHPNLLLEDSNKSCIPVAASNGLVCYRTSSGNFIVSNPVTGSSTELPPLNFASENQSLNAVVMSTTSFNGQISYKIVLVFGELPNLSFKVYNSSSSCWEDENALGRKVDDSSADCDSTDDNVVYFLSKAGNVVASSMQRSPTKQYSSVITNKDGQEVVYFLSSSGTVVACNLTCKCFFEYPRLLPVFCEYSIDIVECNGEMLVVLLSEFLETTSLRVWRFDETNRCWQQIAAMPAAMSHEWYRKKPDINCVGAGSRIFICLNSPELCTYVLCDLVTNNWVELPKCCINGEVMEFMSAFSFEPRIEASV*