AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0148460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148460 MtrunA17_Chr1g0150561 98.015 403 8 0 13 415 1 403 0.0 811
Msa0148460 MtrunA17_Chr3g0122401 25.258 388 241 13 33 413 118 463 3.72e-30 121
Msa0148460 MtrunA17_Chr8g0347881 25.304 411 236 18 29 414 39 403 2.98e-20 92.0
Msa0148460 MtrunA17_Chr1g0161891 22.192 365 221 11 32 390 19 326 5.19e-18 84.7
Msa0148460 MtrunA17_Chr8g0347851 25.441 397 238 19 34 414 44 398 5.90e-13 70.1
Msa0148460 MtrunA17_Chr1g0161871 26.201 229 137 8 32 242 30 244 2.77e-12 67.8
Msa0148460 MtrunA17_Chr7g0220421 22.506 391 243 12 34 415 98 437 3.89e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148460 AT4G33160.1 57.179 397 156 12 29 415 65 457 1.06e-145 422
Msa0148460 AT4G33160.2 57.179 397 156 12 29 415 126 518 4.89e-145 423
Msa0148460 AT1G27340.1 24.528 371 228 12 32 394 118 444 2.44e-26 110
Msa0148460 AT3G61590.4 24.651 430 254 22 14 414 58 446 3.95e-12 68.2
Msa0148460 AT3G61590.2 23.953 430 257 20 14 414 30 418 6.57e-12 67.4
Msa0148460 AT3G61590.3 23.953 430 257 20 14 414 30 418 6.57e-12 67.4
Msa0148460 AT3G61590.1 24.651 430 254 22 14 414 22 410 6.81e-12 67.4

Find 90 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCAAAATGTTGCATTAA+TGG 0.164880 1_4:-8776421 Msa0148460:CDS
ATACAATCTTGTAGGATATT+TGG 0.194528 1_4:+8777055 None:intergenic
CAAACAGAAGCTTCTATTCT+TGG 0.227716 1_4:+8776361 None:intergenic
TCTTGGAAACCACAAGTCTT+AGG 0.228546 1_4:-8776639 Msa0148460:CDS
TCTCCATTAATGCAACATTT+TGG 0.236954 1_4:+8776418 None:intergenic
CACTTATGTTCTTTGTGATT+TGG 0.296690 1_4:-8776464 Msa0148460:CDS
ATTCCTGTTGCAGCTTCTAA+TGG 0.302810 1_4:-8777222 Msa0148460:CDS
CTAGCCGGATATCTTGGTTT+TGG 0.307552 1_4:-8776325 Msa0148460:three_prime_UTR
TGTTCTAAATTTCTGTTCAA+TGG 0.332421 1_4:-8777568 Msa0148460:CDS
ATTGTGTTGGTGCCGGTTCT+CGG 0.340920 1_4:-8776519 Msa0148460:CDS
GTAACCTTGTTCTCATTTAG+TGG 0.345733 1_4:-8776107 Msa0148460:three_prime_UTR
GTGATTTGGTAACCAACAAC+TGG 0.347069 1_4:-8776450 Msa0148460:CDS
CTGTCAACAATCAAATCATT+TGG 0.364285 1_4:+8776164 None:intergenic
ATAGACTTTAAACGAAAGAT+TGG 0.382730 1_4:+8777014 None:intergenic
GAAGACTAATACAATCTTGT+AGG 0.389829 1_4:+8777047 None:intergenic
TCATGCAACATAGCTAGATA+TGG 0.407514 1_4:-8776194 Msa0148460:three_prime_UTR
ATAACTCAAGCTCTTCTTGT+TGG 0.420790 1_4:-8776993 Msa0148460:CDS
TGTTGCAGCTTCTAATGGCC+TGG 0.422631 1_4:-8777217 Msa0148460:CDS
GAATAGAAGCTTCTGTTTGA+CGG 0.426490 1_4:-8776357 Msa0148460:exon
TAACTCAAGCTCTTCTTGTT+GGG 0.426988 1_4:-8776992 Msa0148460:CDS
TACAAGATTGTATTAGTCTT+CGG 0.428289 1_4:-8777045 Msa0148460:CDS
GAAATTCAACGGAGGTAGTT+CGG 0.429403 1_4:+8777134 None:intergenic
AAACTATTGAATCCATTCTC+TGG 0.430777 1_4:+8777522 None:intergenic
CTTGGAAACCACAAGTCTTA+GGG 0.431925 1_4:-8776638 Msa0148460:CDS
TGAAGTTTCCTGACGAAGTT+CGG 0.432189 1_4:+8777187 None:intergenic
TGGGAAGACGAGAATGCTCT+TGG 0.432787 1_4:-8776973 Msa0148460:CDS
GTTTGCTACCGAACTTCGTC+AGG 0.434074 1_4:-8777195 Msa0148460:CDS
AGTGTTTCTTTGAGTACCCT+AGG 0.452698 1_4:-8776741 Msa0148460:CDS
AATGCCCTAGCCGGATATCT+TGG 0.453017 1_4:-8776331 Msa0148460:three_prime_UTR
AAAAGAAAGTCTCCAGAGAA+TGG 0.455501 1_4:-8777534 Msa0148460:CDS
ACATCCCCACAAGGGATCCT+TGG 0.462778 1_4:-8777359 Msa0148460:CDS
TGGCAATTCAACCCAGTTGT+TGG 0.463420 1_4:+8776438 None:intergenic
AGAAGATCCGGTCACAGGAT+TGG 0.466882 1_4:+8777158 None:intergenic
GAATCTTCGAGCAGAAGATT+CGG 0.467832 1_4:+8777258 None:intergenic
TCGATGAGACCAACCGATGC+TGG 0.476739 1_4:-8776606 Msa0148460:CDS
TAGACTTTAAACGAAAGATT+GGG 0.477371 1_4:+8777015 None:intergenic
TTACTTCCGTGTGCAAAAGA+TGG 0.481747 1_4:-8777425 Msa0148460:CDS
ATGACAGACTGGTTGAGATC+GGG 0.482940 1_4:+8777321 None:intergenic
GATATCAATTGTGTTGGTGC+CGG 0.486697 1_4:-8776526 Msa0148460:CDS
GTACTCAAAGAAACACTTGC+AGG 0.487063 1_4:+8776747 None:intergenic
TTGACTCGGCTGAAAACAGT+TGG 0.492513 1_4:-8777296 Msa0148460:CDS
ATAACCAAAACCAAGATATC+CGG 0.496003 1_4:+8776321 None:intergenic
GAATGAAGATGTCTTTGAAA+AGG 0.497404 1_4:-8777484 Msa0148460:CDS
GATGACAGACTGGTTGAGAT+CGG 0.499005 1_4:+8777320 None:intergenic
AGGCCATTAGAAGCTGCAAC+AGG 0.501616 1_4:+8777219 None:intergenic
GCACCAACACAATTGATATC+CGG 0.505766 1_4:+8776529 None:intergenic
AGGTAGTTCGGTAGAAGATC+CGG 0.510241 1_4:+8777146 None:intergenic
CGAGAATGCTCTTGGGAGGA+AGG 0.510339 1_4:-8776965 Msa0148460:CDS
ACCGACGAATATTGCTTGGT+TGG 0.512080 1_4:+8776844 None:intergenic
AATGACCGACGAATATTGCT+TGG 0.513209 1_4:+8776840 None:intergenic
GTGTACTTTCTGAGCAAGGC+TGG 0.518608 1_4:-8776898 Msa0148460:CDS
ATTGATATCGTCGAGTGTAA+CGG 0.526717 1_4:-8776691 Msa0148460:CDS
TCTTTGAAAAGGTACTTTCG+TGG 0.527524 1_4:-8777473 Msa0148460:CDS
ACGAAGTTCGGTAGCAAACC+AGG 0.530457 1_4:+8777199 None:intergenic
CAGCAGCAATGTCTCATGAA+TGG 0.530978 1_4:-8776564 Msa0148460:CDS
AGAACATAAGTGCATAGCTC+AGG 0.532205 1_4:+8776475 None:intergenic
TGTTTGCAATTGAGGTCTCA+AGG 0.532935 1_4:-8777897 Msa0148460:intron
GGGAAGACGAGAATGCTCTT+GGG 0.541458 1_4:-8776972 Msa0148460:CDS
CTGCAATCTGTTGCCAGCAT+CGG 0.543898 1_4:+8776593 None:intergenic
TTGCATTAATGGAGAAGTCA+TGG 0.546480 1_4:-8776410 Msa0148460:CDS
TGACGGTGAAATGCCCTAGC+CGG 0.554753 1_4:-8776340 Msa0148460:three_prime_UTR
AATCTGTTGCCAGCATCGGT+TGG 0.557369 1_4:+8776597 None:intergenic
CCAGTCTGTCATCTTTGACT+CGG 0.557680 1_4:-8777310 Msa0148460:CDS
CAAAACCAAGATATCCGGCT+AGG 0.558173 1_4:+8776326 None:intergenic
TCGGTAGAAGATCCGGTCAC+AGG 0.558565 1_4:+8777153 None:intergenic
ATTGTGTCCAATCCTGTGAC+CGG 0.560669 1_4:-8777165 Msa0148460:CDS
CATTCATGAGACATTGCTGC+TGG 0.561199 1_4:+8776565 None:intergenic
AAACCACAAGTCTTAGGGTG+TGG 0.562665 1_4:-8776633 Msa0148460:CDS
ATTGCAAGCTACAACGGTCC+CGG 0.576687 1_4:+8776774 None:intergenic
CTTGCTCACACATCCCCACA+AGG 0.585521 1_4:-8777368 Msa0148460:CDS
AGACGAAAGAAACTCGAAGT+AGG 0.593990 1_4:+8777447 None:intergenic
TGAATGGTACAGAAAGAAGC+CGG 0.596645 1_4:-8776548 Msa0148460:CDS
TCTGAGCAAGGCTGGAAATG+TGG 0.601419 1_4:-8776890 Msa0148460:CDS
AAGCCGGATATCAATTGTGT+TGG 0.603699 1_4:-8776532 Msa0148460:CDS
AAAACCAAGATATCCGGCTA+GGG 0.604515 1_4:+8776327 None:intergenic
CCGAGTCAAAGATGACAGAC+TGG 0.609122 1_4:+8777310 None:intergenic
AGAAGTCATGGAGTTTATGT+CGG 0.612236 1_4:-8776398 Msa0148460:CDS
AAAGTACACAACATTGTCAT+CGG 0.617043 1_4:+8776912 None:intergenic
TCTCCACACCCTAAGACTTG+TGG 0.621668 1_4:+8776630 None:intergenic
TACTAACAAAGACGGTCAAG+AGG 0.624609 1_4:-8776818 Msa0148460:CDS
TGTTGTGTACTTTCTGAGCA+AGG 0.626293 1_4:-8776902 Msa0148460:CDS
TCCAACCAAGCAATATTCGT+CGG 0.630854 1_4:-8776845 Msa0148460:CDS
GGTCAAATTGCAAGCTACAA+CGG 0.643110 1_4:+8776768 None:intergenic
AAGACGAGAATGCTCTTGGG+AGG 0.645387 1_4:-8776969 Msa0148460:CDS
AAAACCAAGGATCCCTTGTG+GGG 0.654526 1_4:+8777355 None:intergenic
AATACCACTAAATGAGAACA+AGG 0.660886 1_4:+8776103 None:intergenic
TGATTTGGTAACCAACAACT+GGG 0.681708 1_4:-8776449 Msa0148460:CDS
TCGGTCATTACTAACAAAGA+CGG 0.685648 1_4:-8776826 Msa0148460:CDS
TTGCTCACACATCCCCACAA+GGG 0.708158 1_4:-8777367 Msa0148460:CDS
TCTGAGGCGAAATTCAACGG+AGG 0.746256 1_4:+8777126 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGATAACAAAAAAAATTAA+AGG + chr1_4:8776322-8776341 None:intergenic 10.0%
!!! ATTTTTATACTGAAATTAAA+AGG - chr1_4:8776360-8776379 Msa0148460:CDS 10.0%
!!! TTTTTATACTGAAATTAAAA+GGG - chr1_4:8776361-8776380 Msa0148460:CDS 10.0%
!! AAAAAACATAACATAAAAAA+TGG + chr1_4:8777825-8777844 None:intergenic 10.0%
!!! TTGATAACAAAAAAAATTAA+AGG + chr1_4:8776322-8776341 None:intergenic 10.0%
!!! ATTTTTATACTGAAATTAAA+AGG - chr1_4:8776360-8776379 Msa0148460:CDS 10.0%
!!! TTTTTATACTGAAATTAAAA+GGG - chr1_4:8776361-8776380 Msa0148460:CDS 10.0%
!! AAAAAACATAACATAAAAAA+TGG + chr1_4:8777825-8777844 None:intergenic 10.0%
!!! TTTTATACTGAAATTAAAAG+GGG - chr1_4:8776362-8776381 Msa0148460:CDS 15.0%
!!! AAAATTGATTTTTTTGTTGT+TGG - chr1_4:8776490-8776509 Msa0148460:CDS 15.0%
!!! TTTTATACTGAAATTAAAAG+GGG - chr1_4:8776362-8776381 Msa0148460:CDS 15.0%
!!! AAAATTGATTTTTTTGTTGT+TGG - chr1_4:8776490-8776509 Msa0148460:CDS 15.0%
!! CATTAACATATCATGAAAAA+GGG + chr1_4:8776430-8776449 None:intergenic 20.0%
!! ACATTAACATATCATGAAAA+AGG + chr1_4:8776431-8776450 None:intergenic 20.0%
!! CATTAACATATCATGAAAAA+GGG + chr1_4:8776430-8776449 None:intergenic 20.0%
!! ACATTAACATATCATGAAAA+AGG + chr1_4:8776431-8776450 None:intergenic 20.0%
!!! TTTTTACTAGTTCTTGTTTG+AGG - chr1_4:8776165-8776184 Msa0148460:three_prime_UTR 25.0%
!!! TTTTACTAGTTCTTGTTTGA+GGG - chr1_4:8776166-8776185 Msa0148460:three_prime_UTR 25.0%
!!! GAAAGTTTTGAACTTTTTCA+TGG - chr1_4:8776400-8776419 Msa0148460:CDS 25.0%
!! TGTTCTAAATTTCTGTTCAA+TGG - chr1_4:8776549-8776568 Msa0148460:CDS 25.0%
!! ATGAGTACTACATCTTTTAA+TGG - chr1_4:8777039-8777058 Msa0148460:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_4:8777065-8777084 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_4:8777072-8777091 Msa0148460:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_4:8777105-8777124 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_4:8777106-8777125 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_4:8777461-8777480 Msa0148460:CDS 25.0%
! GCTAACAGAACATTAAAAAA+TGG + chr1_4:8777864-8777883 None:intergenic 25.0%
!!! TTTTTACTAGTTCTTGTTTG+AGG - chr1_4:8776165-8776184 Msa0148460:three_prime_UTR 25.0%
!!! TTTTACTAGTTCTTGTTTGA+GGG - chr1_4:8776166-8776185 Msa0148460:three_prime_UTR 25.0%
!!! GAAAGTTTTGAACTTTTTCA+TGG - chr1_4:8776400-8776419 Msa0148460:CDS 25.0%
!! TGTTCTAAATTTCTGTTCAA+TGG - chr1_4:8776549-8776568 Msa0148460:CDS 25.0%
!! ATGAGTACTACATCTTTTAA+TGG - chr1_4:8777039-8777058 Msa0148460:CDS 25.0%
! ATACAATCTTGTAGGATATT+TGG + chr1_4:8777065-8777084 None:intergenic 25.0%
!!! TACAAGATTGTATTAGTCTT+CGG - chr1_4:8777072-8777091 Msa0148460:CDS 25.0%
! TAGACTTTAAACGAAAGATT+GGG + chr1_4:8777105-8777124 None:intergenic 25.0%
! ATAGACTTTAAACGAAAGAT+TGG + chr1_4:8777106-8777125 None:intergenic 25.0%
!!! CGTTTTATTATCAGAATTCT+TGG - chr1_4:8777461-8777480 Msa0148460:CDS 25.0%
! GCTAACAGAACATTAAAAAA+TGG + chr1_4:8777864-8777883 None:intergenic 25.0%
TTTGAGGGTTTGTAGTAAAA+GGG - chr1_4:8776181-8776200 Msa0148460:three_prime_UTR 30.0%
TTGATTACTGTTTGCAATTG+AGG - chr1_4:8776212-8776231 Msa0148460:three_prime_UTR 30.0%
AATAGCTTCTTAAGCTAAGA+GGG - chr1_4:8776269-8776288 Msa0148460:three_prime_UTR 30.0%
ATACTGAAATTAAAAGGGGT+TGG - chr1_4:8776366-8776385 Msa0148460:CDS 30.0%
AAACTATTGAATCCATTCTC+TGG + chr1_4:8776598-8776617 None:intergenic 30.0%
! TGGATTCAATAGTTTTTCAC+TGG - chr1_4:8776603-8776622 Msa0148460:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_4:8776633-8776652 Msa0148460:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_4:8777010-8777029 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_4:8777073-8777092 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_4:8777208-8777227 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_4:8777408-8777427 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_4:8777653-8777672 Msa0148460:intron 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_4:8777696-8777715 Msa0148460:intron 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_4:8777702-8777721 None:intergenic 30.0%
ATAACCAAAACCAAGATATC+CGG + chr1_4:8777799-8777818 None:intergenic 30.0%
CTGTCAACAATCAAATCATT+TGG + chr1_4:8777956-8777975 None:intergenic 30.0%
TTTGAGGGTTTGTAGTAAAA+GGG - chr1_4:8776181-8776200 Msa0148460:three_prime_UTR 30.0%
TTGATTACTGTTTGCAATTG+AGG - chr1_4:8776212-8776231 Msa0148460:three_prime_UTR 30.0%
AATAGCTTCTTAAGCTAAGA+GGG - chr1_4:8776269-8776288 Msa0148460:three_prime_UTR 30.0%
ATACTGAAATTAAAAGGGGT+TGG - chr1_4:8776366-8776385 Msa0148460:CDS 30.0%
AAACTATTGAATCCATTCTC+TGG + chr1_4:8776598-8776617 None:intergenic 30.0%
! TGGATTCAATAGTTTTTCAC+TGG - chr1_4:8776603-8776622 Msa0148460:CDS 30.0%
! GAATGAAGATGTCTTTGAAA+AGG - chr1_4:8776633-8776652 Msa0148460:CDS 30.0%
!! ATTGAGAGATTGATTTTCTG+AGG + chr1_4:8777010-8777029 None:intergenic 30.0%
GAAGACTAATACAATCTTGT+AGG + chr1_4:8777073-8777092 None:intergenic 30.0%
AAAGTACACAACATTGTCAT+CGG + chr1_4:8777208-8777227 None:intergenic 30.0%
! ATAGAGTATTCGCAAAAAAC+TGG + chr1_4:8777408-8777427 None:intergenic 30.0%
CACTTATGTTCTTTGTGATT+TGG - chr1_4:8777653-8777672 Msa0148460:intron 30.0%
TTGCCAAAATGTTGCATTAA+TGG - chr1_4:8777696-8777715 Msa0148460:intron 30.0%
! TCTCCATTAATGCAACATTT+TGG + chr1_4:8777702-8777721 None:intergenic 30.0%
ATAACCAAAACCAAGATATC+CGG + chr1_4:8777799-8777818 None:intergenic 30.0%
CTGTCAACAATCAAATCATT+TGG + chr1_4:8777956-8777975 None:intergenic 30.0%
GTTTGAGGGTTTGTAGTAAA+AGG - chr1_4:8776180-8776199 Msa0148460:three_prime_UTR 35.0%
TTGAGGGTTTGTAGTAAAAG+GGG - chr1_4:8776182-8776201 Msa0148460:three_prime_UTR 35.0%
GAATAGCTTCTTAAGCTAAG+AGG - chr1_4:8776268-8776287 Msa0148460:three_prime_UTR 35.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_4:8776583-8776602 Msa0148460:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_4:8776644-8776663 Msa0148460:CDS 35.0%
! TGATTTCCATCTTTTGCACA+CGG + chr1_4:8776701-8776720 None:intergenic 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_4:8776884-8776903 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_4:8776997-8777016 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_4:8777124-8777143 Msa0148460:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_4:8777125-8777144 Msa0148460:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_4:8777291-8777310 Msa0148460:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_4:8777324-8777343 Msa0148460:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_4:8777325-8777344 Msa0148460:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_4:8777395-8777414 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_4:8777426-8777445 Msa0148460:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_4:8777613-8777632 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_4:8777668-8777687 Msa0148460:intron 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_4:8777707-8777726 Msa0148460:intron 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_4:8777719-8777738 Msa0148460:intron 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_4:8777759-8777778 None:intergenic 35.0%
GAATAGAAGCTTCTGTTTGA+CGG - chr1_4:8777760-8777779 Msa0148460:intron 35.0%
TCATGCAACATAGCTAGATA+TGG - chr1_4:8777923-8777942 Msa0148460:intron 35.0%
GTAACCTTGTTCTCATTTAG+TGG - chr1_4:8778010-8778029 Msa0148460:intron 35.0%
GTTTGAGGGTTTGTAGTAAA+AGG - chr1_4:8776180-8776199 Msa0148460:three_prime_UTR 35.0%
TTGAGGGTTTGTAGTAAAAG+GGG - chr1_4:8776182-8776201 Msa0148460:three_prime_UTR 35.0%
GAATAGCTTCTTAAGCTAAG+AGG - chr1_4:8776268-8776287 Msa0148460:three_prime_UTR 35.0%
AAAAGAAAGTCTCCAGAGAA+TGG - chr1_4:8776583-8776602 Msa0148460:CDS 35.0%
!! TCTTTGAAAAGGTACTTTCG+TGG - chr1_4:8776644-8776663 Msa0148460:CDS 35.0%
! TGATTTCCATCTTTTGCACA+CGG + chr1_4:8776701-8776720 None:intergenic 35.0%
! AACAGGAATGCAACTTTTGT+TGG + chr1_4:8776884-8776903 None:intergenic 35.0%
!!! TTTTCTGAGGCGAAATTCAA+CGG + chr1_4:8776997-8777016 None:intergenic 35.0%
ATAACTCAAGCTCTTCTTGT+TGG - chr1_4:8777124-8777143 Msa0148460:CDS 35.0%
TAACTCAAGCTCTTCTTGTT+GGG - chr1_4:8777125-8777144 Msa0148460:CDS 35.0%
TCGGTCATTACTAACAAAGA+CGG - chr1_4:8777291-8777310 Msa0148460:CDS 35.0%
!!! GTCTATTTTTTGAGTTCGTC+CGG - chr1_4:8777324-8777343 Msa0148460:CDS 35.0%
!!! TCTATTTTTTGAGTTCGTCC+GGG - chr1_4:8777325-8777344 Msa0148460:CDS 35.0%
AAAAAACTGGCAACAACCTA+GGG + chr1_4:8777395-8777414 None:intergenic 35.0%
ATTGATATCGTCGAGTGTAA+CGG - chr1_4:8777426-8777445 Msa0148460:CDS 35.0%
GCATATAAAAATCCGAGAAC+CGG + chr1_4:8777613-8777632 None:intergenic 35.0%
!! TGATTTGGTAACCAACAACT+GGG - chr1_4:8777668-8777687 Msa0148460:intron 35.0%
TTGCATTAATGGAGAAGTCA+TGG - chr1_4:8777707-8777726 Msa0148460:intron 35.0%
AGAAGTCATGGAGTTTATGT+CGG - chr1_4:8777719-8777738 Msa0148460:intron 35.0%
CAAACAGAAGCTTCTATTCT+TGG + chr1_4:8777759-8777778 None:intergenic 35.0%
GAATAGAAGCTTCTGTTTGA+CGG - chr1_4:8777760-8777779 Msa0148460:intron 35.0%
TCATGCAACATAGCTAGATA+TGG - chr1_4:8777923-8777942 Msa0148460:intron 35.0%
TGTTTGCAATTGAGGTCTCA+AGG - chr1_4:8776220-8776239 Msa0148460:three_prime_UTR 40.0%
TAAAAGGGGTTGGAGAATGA+AGG - chr1_4:8776376-8776395 Msa0148460:CDS 40.0%
AAAAGGGGTTGGAGAATGAA+GGG - chr1_4:8776377-8776396 Msa0148460:CDS 40.0%
AGACGAAAGAAACTCGAAGT+AGG + chr1_4:8776673-8776692 None:intergenic 40.0%
TTACTTCCGTGTGCAAAAGA+TGG - chr1_4:8776692-8776711 Msa0148460:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_4:8776766-8776785 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_4:8776768-8776787 Msa0148460:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_4:8776862-8776881 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_4:8776895-8776914 Msa0148460:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_4:8776933-8776952 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_4:8776986-8777005 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_4:8777215-8777234 Msa0148460:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_4:8777272-8777291 Msa0148460:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_4:8777280-8777299 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_4:8777299-8777318 Msa0148460:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_4:8777352-8777371 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_4:8777373-8777392 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_4:8777376-8777395 Msa0148460:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_4:8777396-8777415 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_4:8777478-8777497 Msa0148460:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_4:8777479-8777498 Msa0148460:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_4:8777569-8777588 Msa0148460:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_4:8777585-8777604 Msa0148460:intron 40.0%
GCACCAACACAATTGATATC+CGG + chr1_4:8777591-8777610 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_4:8777591-8777610 Msa0148460:intron 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_4:8777645-8777664 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_4:8777667-8777686 Msa0148460:intron 40.0%
AAAACCAAGATATCCGGCTA+GGG + chr1_4:8777793-8777812 None:intergenic 40.0%
TGTTTGCAATTGAGGTCTCA+AGG - chr1_4:8776220-8776239 Msa0148460:three_prime_UTR 40.0%
TAAAAGGGGTTGGAGAATGA+AGG - chr1_4:8776376-8776395 Msa0148460:CDS 40.0%
AAAAGGGGTTGGAGAATGAA+GGG - chr1_4:8776377-8776396 Msa0148460:CDS 40.0%
AGACGAAAGAAACTCGAAGT+AGG + chr1_4:8776673-8776692 None:intergenic 40.0%
TTACTTCCGTGTGCAAAAGA+TGG - chr1_4:8776692-8776711 Msa0148460:CDS 40.0%
AAAAACCAAGGATCCCTTGT+GGG + chr1_4:8776766-8776785 None:intergenic 40.0%
!! AAGGGATCCTTGGTTTTTCA+TGG - chr1_4:8776768-8776787 Msa0148460:CDS 40.0%
GAATCTTCGAGCAGAAGATT+CGG + chr1_4:8776862-8776881 None:intergenic 40.0%
ATTCCTGTTGCAGCTTCTAA+TGG - chr1_4:8776895-8776914 Msa0148460:CDS 40.0%
TGAAGTTTCCTGACGAAGTT+CGG + chr1_4:8776933-8776952 None:intergenic 40.0%
GAAATTCAACGGAGGTAGTT+CGG + chr1_4:8776986-8777005 None:intergenic 40.0%
TGTTGTGTACTTTCTGAGCA+AGG - chr1_4:8777215-8777234 Msa0148460:CDS 40.0%
TCCAACCAAGCAATATTCGT+CGG - chr1_4:8777272-8777291 Msa0148460:CDS 40.0%
AATGACCGACGAATATTGCT+TGG + chr1_4:8777280-8777299 None:intergenic 40.0%
TACTAACAAAGACGGTCAAG+AGG - chr1_4:8777299-8777318 Msa0148460:CDS 40.0%
GGTCAAATTGCAAGCTACAA+CGG + chr1_4:8777352-8777371 None:intergenic 40.0%
GTACTCAAAGAAACACTTGC+AGG + chr1_4:8777373-8777392 None:intergenic 40.0%
AGTGTTTCTTTGAGTACCCT+AGG - chr1_4:8777376-8777395 Msa0148460:CDS 40.0%
CAAAAAACTGGCAACAACCT+AGG + chr1_4:8777396-8777415 None:intergenic 40.0%
TCTTGGAAACCACAAGTCTT+AGG - chr1_4:8777478-8777497 Msa0148460:CDS 40.0%
CTTGGAAACCACAAGTCTTA+GGG - chr1_4:8777479-8777498 Msa0148460:CDS 40.0%
TGAATGGTACAGAAAGAAGC+CGG - chr1_4:8777569-8777588 Msa0148460:CDS 40.0%
AAGCCGGATATCAATTGTGT+TGG - chr1_4:8777585-8777604 Msa0148460:intron 40.0%
GCACCAACACAATTGATATC+CGG + chr1_4:8777591-8777610 None:intergenic 40.0%
! GATATCAATTGTGTTGGTGC+CGG - chr1_4:8777591-8777610 Msa0148460:intron 40.0%
AGAACATAAGTGCATAGCTC+AGG + chr1_4:8777645-8777664 None:intergenic 40.0%
!! GTGATTTGGTAACCAACAAC+TGG - chr1_4:8777667-8777686 Msa0148460:intron 40.0%
AAAACCAAGATATCCGGCTA+GGG + chr1_4:8777793-8777812 None:intergenic 40.0%
AAAGGGGTTGGAGAATGAAG+GGG - chr1_4:8776378-8776397 Msa0148460:CDS 45.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_4:8776765-8776784 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_4:8776767-8776786 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_4:8776778-8776797 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_4:8776799-8776818 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_4:8776800-8776819 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_4:8776807-8776826 Msa0148460:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_4:8776821-8776840 Msa0148460:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_4:8776952-8776971 Msa0148460:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_4:8776974-8776993 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_4:8777276-8777295 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_4:8777484-8777503 Msa0148460:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_4:8777555-8777574 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_4:8777553-8777572 Msa0148460:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_4:8777682-8777701 None:intergenic 45.0%
CAAAACCAAGATATCCGGCT+AGG + chr1_4:8777794-8777813 None:intergenic 45.0%
! CTAGCCGGATATCTTGGTTT+TGG - chr1_4:8777792-8777811 Msa0148460:intron 45.0%
AAAGGGGTTGGAGAATGAAG+GGG - chr1_4:8776378-8776397 Msa0148460:CDS 45.0%
AAAACCAAGGATCCCTTGTG+GGG + chr1_4:8776765-8776784 None:intergenic 45.0%
GAAAAACCAAGGATCCCTTG+TGG + chr1_4:8776767-8776786 None:intergenic 45.0%
GGAGCAACCATGAAAAACCA+AGG + chr1_4:8776778-8776797 None:intergenic 45.0%
! ATGACAGACTGGTTGAGATC+GGG + chr1_4:8776799-8776818 None:intergenic 45.0%
! GATGACAGACTGGTTGAGAT+CGG + chr1_4:8776800-8776819 None:intergenic 45.0%
CCAGTCTGTCATCTTTGACT+CGG - chr1_4:8776807-8776826 Msa0148460:CDS 45.0%
!! TTGACTCGGCTGAAAACAGT+TGG - chr1_4:8776821-8776840 Msa0148460:CDS 45.0%
ATTGTGTCCAATCCTGTGAC+CGG - chr1_4:8776952-8776971 Msa0148460:CDS 45.0%
AGGTAGTTCGGTAGAAGATC+CGG + chr1_4:8776974-8776993 None:intergenic 45.0%
ACCGACGAATATTGCTTGGT+TGG + chr1_4:8777276-8777295 None:intergenic 45.0%
AAACCACAAGTCTTAGGGTG+TGG - chr1_4:8777484-8777503 Msa0148460:CDS 45.0%
CATTCATGAGACATTGCTGC+TGG + chr1_4:8777555-8777574 None:intergenic 45.0%
CAGCAGCAATGTCTCATGAA+TGG - chr1_4:8777553-8777572 Msa0148460:CDS 45.0%
TGGCAATTCAACCCAGTTGT+TGG + chr1_4:8777682-8777701 None:intergenic 45.0%
CAAAACCAAGATATCCGGCT+AGG + chr1_4:8777794-8777813 None:intergenic 45.0%
! CTAGCCGGATATCTTGGTTT+TGG - chr1_4:8777792-8777811 Msa0148460:intron 45.0%
TTGCTCACACATCCCCACAA+GGG - chr1_4:8776750-8776769 Msa0148460:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_4:8776810-8776829 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_4:8776901-8776920 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_4:8776900-8776919 Msa0148460:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_4:8776921-8776940 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_4:8776922-8776941 Msa0148460:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_4:8776962-8776981 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_4:8776994-8777013 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_4:8777144-8777163 Msa0148460:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_4:8777145-8777164 Msa0148460:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_4:8777148-8777167 Msa0148460:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_4:8777219-8777238 Msa0148460:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_4:8777227-8777246 Msa0148460:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_4:8777346-8777365 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_4:8777490-8777509 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_4:8777523-8777542 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_4:8777527-8777546 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_4:8777598-8777617 Msa0148460:intron 50.0%
AATGCCCTAGCCGGATATCT+TGG - chr1_4:8777786-8777805 Msa0148460:intron 50.0%
TTGCTCACACATCCCCACAA+GGG - chr1_4:8776750-8776769 Msa0148460:CDS 50.0%
CCGAGTCAAAGATGACAGAC+TGG + chr1_4:8776810-8776829 None:intergenic 50.0%
AGGCCATTAGAAGCTGCAAC+AGG + chr1_4:8776901-8776920 None:intergenic 50.0%
TGTTGCAGCTTCTAATGGCC+TGG - chr1_4:8776900-8776919 Msa0148460:CDS 50.0%
ACGAAGTTCGGTAGCAAACC+AGG + chr1_4:8776921-8776940 None:intergenic 50.0%
! GTTTGCTACCGAACTTCGTC+AGG - chr1_4:8776922-8776941 Msa0148460:CDS 50.0%
AGAAGATCCGGTCACAGGAT+TGG + chr1_4:8776962-8776981 None:intergenic 50.0%
! TCTGAGGCGAAATTCAACGG+AGG + chr1_4:8776994-8777013 None:intergenic 50.0%
TGGGAAGACGAGAATGCTCT+TGG - chr1_4:8777144-8777163 Msa0148460:CDS 50.0%
GGGAAGACGAGAATGCTCTT+GGG - chr1_4:8777145-8777164 Msa0148460:CDS 50.0%
AAGACGAGAATGCTCTTGGG+AGG - chr1_4:8777148-8777167 Msa0148460:CDS 50.0%
GTGTACTTTCTGAGCAAGGC+TGG - chr1_4:8777219-8777238 Msa0148460:CDS 50.0%
TCTGAGCAAGGCTGGAAATG+TGG - chr1_4:8777227-8777246 Msa0148460:CDS 50.0%
ATTGCAAGCTACAACGGTCC+CGG + chr1_4:8777346-8777365 None:intergenic 50.0%
TCTCCACACCCTAAGACTTG+TGG + chr1_4:8777490-8777509 None:intergenic 50.0%
!! AATCTGTTGCCAGCATCGGT+TGG + chr1_4:8777523-8777542 None:intergenic 50.0%
CTGCAATCTGTTGCCAGCAT+CGG + chr1_4:8777527-8777546 None:intergenic 50.0%
!! ATTGTGTTGGTGCCGGTTCT+CGG - chr1_4:8777598-8777617 Msa0148460:intron 50.0%
AATGCCCTAGCCGGATATCT+TGG - chr1_4:8777786-8777805 Msa0148460:intron 50.0%
CTTGCTCACACATCCCCACA+AGG - chr1_4:8776749-8776768 Msa0148460:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_4:8776758-8776777 Msa0148460:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_4:8776967-8776986 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_4:8777152-8777171 Msa0148460:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_4:8777511-8777530 Msa0148460:CDS 55.0%
TGACGGTGAAATGCCCTAGC+CGG - chr1_4:8777777-8777796 Msa0148460:intron 55.0%
CTTGCTCACACATCCCCACA+AGG - chr1_4:8776749-8776768 Msa0148460:CDS 55.0%
ACATCCCCACAAGGGATCCT+TGG - chr1_4:8776758-8776777 Msa0148460:CDS 55.0%
TCGGTAGAAGATCCGGTCAC+AGG + chr1_4:8776967-8776986 None:intergenic 55.0%
CGAGAATGCTCTTGGGAGGA+AGG - chr1_4:8777152-8777171 Msa0148460:CDS 55.0%
TCGATGAGACCAACCGATGC+TGG - chr1_4:8777511-8777530 Msa0148460:CDS 55.0%
TGACGGTGAAATGCCCTAGC+CGG - chr1_4:8777777-8777796 Msa0148460:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 8776107 8778032 8776107 ID=Msa0148460;Name=Msa0148460
chr1_4 mRNA 8776107 8778032 8776107 ID=Msa0148460-mRNA-1;Parent=Msa0148460;Name=Msa0148460-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0.33|0.33|1|1|3|253|415
chr1_4 exon 8776107 8777595 8776107 ID=Msa0148460-mRNA-1:exon:3712;Parent=Msa0148460-mRNA-1
chr1_4 exon 8777898 8777905 8777898 ID=Msa0148460-mRNA-1:exon:3711;Parent=Msa0148460-mRNA-1
chr1_4 exon 8778029 8778032 8778029 ID=Msa0148460-mRNA-1:exon:3710;Parent=Msa0148460-mRNA-1
chr1_4 CDS 8778029 8778032 8778029 ID=Msa0148460-mRNA-1:cds;Parent=Msa0148460-mRNA-1
chr1_4 CDS 8777898 8777905 8777898 ID=Msa0148460-mRNA-1:cds;Parent=Msa0148460-mRNA-1
chr1_4 CDS 8776360 8777595 8776360 ID=Msa0148460-mRNA-1:cds;Parent=Msa0148460-mRNA-1
chr1_4 three_prime_UTR 8776107 8776359 8776107 ID=Msa0148460-mRNA-1:three_prime_utr;Parent=Msa0148460-mRNA-1
Gene Sequence

>Msa0148460

ATGTGTCTCAAGTTGTTTGTTCTAAATTTCTGTTCAATGGAACATAGATTGAAAAGAAAGTCTCCAGAGAATGGATTCAATAGTTTTTCACTGGATGATCTGAATGAAGATGTCTTTGAAAAGGTACTTTCGTGGTTACCTACTTCGAGTTTCTTTCGTCTTACTTCCGTGTGCAAAAGATGGAAATCAGCTGCTGATTCTGCGAGTTTCAAGCTCGCTTGCTCACACATCCCCACAAGGGATCCTTGGTTTTTCATGGTTGCTCCCGATCTCAACCAGTCTGTCATCTTTGACTCGGCTGAAAACAGTTGGAAAAGACTCAATCATCCGAATCTTCTGCTCGAAGATTCCAACAAAAGTTGCATTCCTGTTGCAGCTTCTAATGGCCTGGTTTGCTACCGAACTTCGTCAGGAAACTTCATTGTGTCCAATCCTGTGACCGGATCTTCTACCGAACTACCTCCGTTGAATTTCGCCTCAGAAAATCAATCTCTCAATGCTGTTGTTATGAGTACTACATCTTTTAATGGCCAAATATCCTACAAGATTGTATTAGTCTTCGGTGAACTGCCCAATCTTTCGTTTAAAGTCTATAACTCAAGCTCTTCTTGTTGGGAAGACGAGAATGCTCTTGGGAGGAAGGTCGATGATAGTTCAGCTGATTGCGATTCAACCGATGACAATGTTGTGTACTTTCTGAGCAAGGCTGGAAATGTGGTAGCAAGTAGCATGCAGAGAAGTCCAACCAAGCAATATTCGTCGGTCATTACTAACAAAGACGGTCAAGAGGTAGTCTATTTTTTGAGTTCGTCCGGGACCGTTGTAGCTTGCAATTTGACCTGCAAGTGTTTCTTTGAGTACCCTAGGTTGTTGCCAGTTTTTTGCGAATACTCTATTGATATCGTCGAGTGTAACGGTGAGATGTTAGTCGTTTTATTATCAGAATTCTTGGAAACCACAAGTCTTAGGGTGTGGAGATTCGATGAGACCAACCGATGCTGGCAACAGATTGCAGCAATGCCAGCAGCAATGTCTCATGAATGGTACAGAAAGAAGCCGGATATCAATTGTGTTGGTGCCGGTTCTCGGATTTTTATATGCTTGAACTCTCCTGAGCTATGCACTTATGTTCTTTGTGATTTGGTAACCAACAACTGGGTTGAATTGCCAAAATGTTGCATTAATGGAGAAGTCATGGAGTTTATGTCGGCATTTTCTTTCGAGCCAAGAATAGAAGCTTCTGTTTGA
Protein sequence

>Msa0148460

MCLKLFVLNFCSMEHRLKRKSPENGFNSFSLDDLNEDVFEKVLSWLPTSSFFRLTSVCKRWKSAADSASFKLACSHIPTRDPWFFMVAPDLNQSVIFDSAENSWKRLNHPNLLLEDSNKSCIPVAASNGLVCYRTSSGNFIVSNPVTGSSTELPPLNFASENQSLNAVVMSTTSFNGQISYKIVLVFGELPNLSFKVYNSSSSCWEDENALGRKVDDSSADCDSTDDNVVYFLSKAGNVVASSMQRSPTKQYSSVITNKDGQEVVYFLSSSGTVVACNLTCKCFFEYPRLLPVFCEYSIDIVECNGEMLVVLLSEFLETTSLRVWRFDETNRCWQQIAAMPAAMSHEWYRKKPDINCVGAGSRIFICLNSPELCTYVLCDLVTNNWVELPKCCINGEVMEFMSAFSFEPRIEASV*