AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054320 MtrunA17_Chr1g0150281 94.737 285 14 1 1 284 1 285 0.0 543
Msa0054320 MtrunA17_Chr3g0136961 58.108 296 93 9 1 275 1 286 3.15e-101 298
Msa0054320 MtrunA17_Chr2g0333851 46.067 267 118 7 24 275 58 313 9.20e-65 205
Msa0054320 MtrunA17_Chr4g0001361 38.390 323 137 10 3 275 4 314 2.09e-51 171
Msa0054320 MtrunA17_Chr1g0150271 35.741 263 130 5 21 276 8 238 1.83e-41 143
Msa0054320 MtrunA17_Chr7g0270831 33.333 216 106 5 6 196 71 273 1.59e-26 107
Msa0054320 MtrunA17_Chr2g0288871 32.864 213 99 4 28 196 150 362 4.35e-26 107
Msa0054320 MtrunA17_Chr3g0099641 33.010 206 80 5 124 271 236 441 3.07e-24 101
Msa0054320 MtrunA17_Chr4g0060271 57.333 75 32 0 122 196 238 312 7.05e-24 101
Msa0054320 MtrunA17_Chr4g0051181 52.564 78 37 0 119 196 223 300 7.35e-24 100
Msa0054320 MtrunA17_Chr7g0216891 46.739 92 49 0 122 213 202 293 1.97e-23 99.8
Msa0054320 MtrunA17_Chr4g0075201 30.144 209 101 5 25 196 124 324 5.44e-23 98.2
Msa0054320 MtrunA17_Chr2g0322391 47.778 90 47 0 124 213 334 423 6.16e-23 98.2
Msa0054320 MtrunA17_Chr2g0302391 41.739 115 56 3 91 196 156 268 7.77e-23 97.4
Msa0054320 MtrunA17_Chr4g0025051 42.000 100 58 0 116 215 312 411 1.53e-20 91.3
Msa0054320 MtrunA17_Chr3g0101131 34.483 145 82 5 62 196 330 471 2.59e-20 90.5
Msa0054320 MtrunA17_Chr5g0445311 57.895 57 23 1 140 196 365 420 1.74e-19 88.2
Msa0054320 MtrunA17_Chr5g0445311 31.788 151 85 5 79 213 129 277 8.88e-11 62.0
Msa0054320 MtrunA17_Chr1g0194481 48.649 74 33 2 123 196 146 214 2.15e-19 86.3
Msa0054320 MtrunA17_Chr4g0025061 50.685 73 36 0 124 196 345 417 2.64e-19 87.8
Msa0054320 MtrunA17_Chr5g0447701 52.703 74 32 2 124 197 239 309 2.90e-19 86.7
Msa0054320 MtrunA17_Chr5g0443131 47.297 74 34 2 123 196 145 213 3.84e-19 85.5
Msa0054320 MtrunA17_Chr4g0051881 57.895 57 23 1 140 196 179 234 4.52e-19 85.5
Msa0054320 MtrunA17_Chr7g0270191 48.052 77 37 2 120 196 128 201 5.28e-19 84.7
Msa0054320 MtrunA17_Chr5g0401821 52.632 57 27 0 140 196 200 256 6.66e-19 84.3
Msa0054320 MtrunA17_Chr1g0147881 36.538 104 55 1 93 196 227 319 1.12e-18 84.7
Msa0054320 MtrunA17_Chr5g0418781 47.297 74 34 2 123 196 112 180 1.82e-18 83.2
Msa0054320 MtrunA17_Chr1g0184691 47.945 73 36 1 127 197 279 351 2.54e-18 84.0
Msa0054320 MtrunA17_Chr2g0294231 47.297 74 34 2 123 196 191 259 3.48e-18 83.6
Msa0054320 MtrunA17_Chr5g0432161 47.945 73 35 2 124 196 408 477 3.59e-18 84.3
Msa0054320 MtrunA17_Chr5g0432161 50.877 57 26 2 140 196 245 299 1.25e-13 70.9
Msa0054320 MtrunA17_Chr7g0263531 50.746 67 32 1 130 196 352 417 3.70e-18 84.0
Msa0054320 MtrunA17_Chr7g0263531 56.897 58 23 2 140 197 190 245 6.43e-16 77.4
Msa0054320 MtrunA17_Chr4g0053281 47.692 65 34 0 140 204 267 331 4.46e-18 82.8
Msa0054320 MtrunA17_Chr5g0418191 47.945 73 35 2 124 196 307 376 4.78e-18 83.6
Msa0054320 MtrunA17_Chr5g0418191 52.632 57 25 2 140 196 152 206 1.36e-13 70.5
Msa0054320 MtrunA17_Chr4g0042831 46.341 82 38 3 115 196 259 334 7.40e-18 82.8
Msa0054320 MtrunA17_Chr4g0042831 47.368 57 29 1 140 196 146 201 7.62e-11 62.0
Msa0054320 MtrunA17_Chr7g0228941 52.632 57 27 0 140 196 53 109 8.26e-18 77.8
Msa0054320 MtrunA17_Chr7g0218081 47.945 73 35 2 124 196 397 466 1.98e-17 82.0
Msa0054320 MtrunA17_Chr7g0218081 49.123 57 27 2 140 196 229 283 1.91e-11 64.3
Msa0054320 MtrunA17_Chr1g0188941 50.877 57 27 1 140 196 52 107 2.48e-17 76.3
Msa0054320 MtrunA17_Chr7g0238921 49.333 75 37 1 140 214 125 198 3.01e-17 80.1
Msa0054320 MtrunA17_Chr3g0106491 46.575 73 36 2 124 196 352 421 4.23e-17 80.9
Msa0054320 MtrunA17_Chr3g0106491 50.877 57 26 2 140 196 199 253 3.23e-13 69.3
Msa0054320 MtrunA17_Chr8g0379461 51.724 58 28 0 140 197 77 134 7.10e-17 78.2
Msa0054320 MtrunA17_Chr1g0150801 50.877 57 27 1 140 196 103 158 1.35e-16 75.5
Msa0054320 MtrunA17_Chr4g0004911 41.304 92 48 3 105 196 307 392 1.75e-16 79.3
Msa0054320 MtrunA17_Chr4g0004911 47.368 57 29 1 140 196 185 240 7.15e-13 68.6
Msa0054320 MtrunA17_Chr3g0139021 52.632 57 27 0 140 196 315 371 2.15e-16 78.6
Msa0054320 MtrunA17_Chr7g0224801 51.613 62 29 1 140 201 94 154 2.43e-16 75.1
Msa0054320 MtrunA17_Chr7g0249301 44.737 76 39 2 121 196 276 348 2.84e-16 78.2
Msa0054320 MtrunA17_Chr4g0071521 50.000 58 29 0 140 197 246 303 2.91e-16 77.4
Msa0054320 MtrunA17_Chr8g0383351 52.632 57 26 1 140 196 161 216 2.95e-16 76.3
Msa0054320 MtrunA17_Chr2g0284321 49.123 57 29 0 140 196 36 92 3.00e-16 75.9
Msa0054320 MtrunA17_Chr3g0129361 52.632 57 26 1 140 196 145 200 3.03e-16 75.9
Msa0054320 MtrunA17_Chr7g0249311 33.540 161 89 7 113 262 365 518 4.47e-16 78.2
Msa0054320 MtrunA17_Chr3g0079691 46.575 73 36 2 124 196 482 551 6.07e-16 77.8
Msa0054320 MtrunA17_Chr3g0079691 50.877 57 26 2 140 196 281 335 5.58e-12 65.9
Msa0054320 MtrunA17_Chr7g0243891 45.205 73 37 2 124 196 361 430 7.12e-16 77.4
Msa0054320 MtrunA17_Chr7g0243891 47.368 57 28 2 140 196 217 271 6.69e-11 62.4
Msa0054320 MtrunA17_Chr4g0068541 45.205 73 37 2 124 196 195 264 7.41e-16 77.0
Msa0054320 MtrunA17_Chr2g0289181 34.426 122 73 3 140 254 15 136 8.36e-16 75.1
Msa0054320 MtrunA17_Chr8g0335811 52.632 57 26 1 140 196 155 210 1.62e-15 73.9
Msa0054320 MtrunA17_Chr2g0319961 52.632 57 27 0 140 196 81 137 2.43e-15 73.9
Msa0054320 MtrunA17_Chr4g0074001 50.877 57 27 1 140 196 360 415 5.66e-15 74.7
Msa0054320 MtrunA17_Chr8g0386241 46.774 62 32 1 140 201 104 164 6.34e-15 71.2
Msa0054320 MtrunA17_Chr2g0293481 50.877 57 28 0 140 196 268 324 1.42e-14 73.6
Msa0054320 MtrunA17_Chr8g0387501 49.123 57 29 0 140 196 130 186 2.13e-14 71.6
Msa0054320 MtrunA17_Chr3g0129321 49.123 57 28 1 140 196 119 174 3.21e-14 70.1
Msa0054320 MtrunA17_Chr8g0348311 45.614 57 31 0 140 196 156 212 5.77e-14 71.2
Msa0054320 MtrunA17_Chr7g0248721 52.459 61 27 2 140 200 142 200 6.58e-14 69.3
Msa0054320 MtrunA17_Chr7g0270051 47.368 57 30 0 140 196 54 110 7.41e-14 70.1
Msa0054320 MtrunA17_Chr7g0248691 47.368 57 30 0 140 196 155 211 1.39e-13 69.7
Msa0054320 MtrunA17_Chr8g0383251 45.614 57 30 1 140 196 109 164 1.41e-13 67.8
Msa0054320 MtrunA17_Chr4g0024391 34.783 92 60 0 124 215 214 305 1.68e-13 70.5
Msa0054320 MtrunA17_Chr6g0485431 38.636 88 48 4 111 197 130 212 3.10e-13 68.9
Msa0054320 MtrunA17_Chr1g0202461 36.842 76 48 0 140 215 262 337 4.94e-13 68.6
Msa0054320 MtrunA17_Chr1g0189391 41.333 75 40 3 124 197 84 155 6.38e-13 68.2
Msa0054320 MtrunA17_Chr8g0387981 42.667 75 41 1 140 212 133 207 2.88e-12 66.2
Msa0054320 MtrunA17_Chr3g0126391 50.877 57 26 1 140 194 134 190 6.84e-12 65.1
Msa0054320 MtrunA17_Chr5g0447711 46.479 71 36 2 126 196 218 286 7.41e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054320 AT1G80840.1 41.967 305 138 9 3 278 6 300 2.28e-67 212
Msa0054320 AT2G25000.1 40.636 283 122 7 6 278 23 269 1.63e-59 191
Msa0054320 AT2G25000.2 41.603 262 110 6 24 278 1 226 5.00e-59 188
Msa0054320 AT2G25000.4 41.603 262 110 6 24 278 1 226 5.00e-59 188
Msa0054320 AT2G25000.3 41.509 265 112 6 21 278 20 248 1.36e-58 187
Msa0054320 AT4G31800.1 40.379 317 136 10 3 278 4 308 2.25e-58 189
Msa0054320 AT4G31800.2 53.529 170 69 4 111 278 82 243 4.55e-52 171
Msa0054320 AT4G31800.3 39.720 214 85 7 3 176 4 213 3.77e-32 119
Msa0054320 AT4G04450.2 47.826 92 48 0 124 215 183 274 6.59e-24 100
Msa0054320 AT4G04450.1 47.826 92 48 0 124 215 277 368 1.62e-23 100
Msa0054320 AT4G22070.4 54.795 73 33 0 124 196 282 354 5.07e-23 99.0
Msa0054320 AT4G22070.3 54.795 73 33 0 124 196 282 354 5.07e-23 99.0
Msa0054320 AT4G22070.2 54.795 73 33 0 124 196 282 354 5.07e-23 99.0
Msa0054320 AT4G22070.1 54.795 73 33 0 124 196 282 354 5.07e-23 99.0
Msa0054320 AT4G01720.1 32.995 197 99 4 28 196 105 296 5.21e-23 98.6
Msa0054320 AT1G68150.1 49.425 87 44 0 124 210 220 306 5.67e-23 97.4
Msa0054320 AT1G62300.1 54.795 73 33 0 124 196 297 369 7.94e-23 98.2
Msa0054320 AT5G15130.2 54.054 74 34 0 140 213 186 259 1.53e-22 97.4
Msa0054320 AT5G15130.1 54.054 74 34 0 140 213 228 301 2.04e-22 97.1
Msa0054320 AT1G18860.1 64.912 57 20 0 140 196 240 296 1.97e-21 94.4
Msa0054320 AT1G64000.1 57.377 61 25 1 140 200 115 174 3.47e-19 83.6
Msa0054320 AT4G18170.1 53.333 60 27 1 137 196 170 228 7.18e-19 85.1
Msa0054320 AT1G29860.1 53.333 60 27 1 137 196 134 192 7.37e-19 84.7
Msa0054320 AT2G04880.2 57.627 59 24 1 138 196 282 339 1.07e-18 85.9
Msa0054320 AT2G04880.1 57.627 59 24 1 138 196 306 363 1.18e-18 85.9
Msa0054320 AT5G41570.1 57.377 61 25 1 140 200 99 158 1.48e-18 81.6
Msa0054320 AT2G47260.1 55.000 60 26 1 137 196 172 230 1.90e-18 84.3
Msa0054320 AT4G26640.5 49.315 73 34 2 124 196 192 261 2.25e-18 84.7
Msa0054320 AT4G26640.6 49.315 73 34 2 124 196 192 261 2.25e-18 84.7
Msa0054320 AT4G26640.4 49.315 73 34 2 124 196 192 261 2.25e-18 84.7
Msa0054320 AT4G26640.3 49.315 73 34 2 124 196 192 261 2.25e-18 84.7
Msa0054320 AT5G07100.2 38.614 101 58 3 96 196 101 197 2.27e-18 82.0
Msa0054320 AT5G07100.2 45.070 71 38 1 126 196 11 80 2.32e-13 68.2
Msa0054320 AT5G07100.4 38.614 101 58 3 96 196 101 197 2.27e-18 82.0
Msa0054320 AT5G07100.4 45.070 71 38 1 126 196 11 80 2.32e-13 68.2
Msa0054320 AT5G07100.5 38.614 101 58 3 96 196 101 197 2.27e-18 82.0
Msa0054320 AT5G07100.5 45.070 71 38 1 126 196 11 80 2.32e-13 68.2
Msa0054320 AT5G07100.3 38.614 101 58 3 96 196 101 197 2.27e-18 82.0
Msa0054320 AT5G07100.3 45.070 71 38 1 126 196 11 80 2.32e-13 68.2
Msa0054320 AT5G26170.1 50.877 57 27 1 140 196 114 169 2.32e-18 80.9
Msa0054320 AT1G13960.2 47.945 73 35 2 124 196 243 312 3.34e-18 84.0
Msa0054320 AT1G13960.2 50.877 57 26 2 140 196 77 131 2.07e-12 67.0
Msa0054320 AT4G26640.1 56.140 57 24 1 140 196 310 365 3.64e-18 84.3
Msa0054320 AT4G26640.7 56.140 57 24 1 140 196 342 397 3.81e-18 84.7
Msa0054320 AT4G26640.2 56.140 57 24 1 140 196 382 437 3.88e-18 84.7
Msa0054320 AT1G13960.1 47.945 73 35 2 124 196 396 465 4.93e-18 84.3
Msa0054320 AT1G13960.1 50.877 57 26 2 140 196 230 284 2.14e-12 67.4
Msa0054320 AT2G03340.1 47.945 73 35 2 124 196 402 471 5.41e-18 84.0
Msa0054320 AT2G03340.1 50.877 57 26 2 140 196 251 305 3.27e-13 69.7
Msa0054320 AT5G46350.1 47.222 72 33 2 125 196 173 239 6.16e-18 82.8
Msa0054320 AT5G49520.1 47.222 72 34 2 140 208 222 292 7.41e-18 83.2
Msa0054320 AT5G07100.1 38.614 101 58 3 96 196 194 290 9.08e-18 82.0
Msa0054320 AT5G07100.1 45.070 71 38 1 126 196 104 173 8.10e-13 67.8
Msa0054320 AT4G31550.1 50.877 57 28 0 140 196 247 303 1.90e-17 81.3
Msa0054320 AT4G31550.2 50.877 57 28 0 140 196 246 302 1.94e-17 81.3
Msa0054320 AT2G30590.1 52.632 57 27 0 140 196 314 370 2.25e-17 81.6
Msa0054320 AT5G13080.1 54.839 62 27 1 140 201 68 128 2.33e-17 77.4
Msa0054320 AT1G69310.4 54.386 57 25 1 140 196 148 203 2.60e-17 80.5
Msa0054320 AT1G69310.2 54.386 57 25 1 140 196 148 203 2.60e-17 80.5
Msa0054320 AT1G69310.1 54.386 57 25 1 140 196 148 203 2.60e-17 80.5
Msa0054320 AT2G23320.1 49.123 57 29 0 140 196 241 297 2.68e-17 80.9
Msa0054320 AT5G28650.1 44.186 86 40 2 127 210 248 327 3.10e-17 80.9
Msa0054320 AT1G69810.2 50.000 74 36 1 124 196 130 203 3.19e-17 80.9
Msa0054320 AT5G56270.1 47.945 73 35 2 124 196 474 543 3.58e-17 82.0
Msa0054320 AT5G56270.1 50.877 57 26 2 140 196 274 328 3.51e-11 63.9
Msa0054320 AT3G62340.1 51.562 64 30 1 133 196 112 174 4.15e-17 79.7
Msa0054320 AT1G69810.1 50.000 74 36 1 124 196 188 261 4.55e-17 80.9
Msa0054320 AT3G01080.1 46.575 73 36 2 124 196 293 362 6.78e-17 80.5
Msa0054320 AT3G01080.1 49.123 57 27 2 140 196 168 222 9.25e-11 62.4
Msa0054320 AT3G01080.2 46.575 73 36 2 124 196 331 400 7.16e-17 80.5
Msa0054320 AT3G01080.2 49.123 57 27 2 140 196 206 260 8.41e-11 62.4
Msa0054320 AT1G29280.1 50.877 57 28 0 140 196 75 131 1.09e-16 78.2
Msa0054320 AT2G24570.1 50.877 57 28 0 140 196 244 300 1.37e-16 79.0
Msa0054320 AT3G04670.1 44.444 72 38 1 127 196 248 319 1.55e-16 79.0
Msa0054320 AT2G38470.1 45.205 73 37 2 124 196 349 418 2.53e-16 79.3
Msa0054320 AT2G38470.1 50.877 57 26 2 140 196 185 239 4.19e-13 69.7
Msa0054320 AT4G24240.1 46.552 58 31 0 140 197 282 339 4.55e-16 77.8
Msa0054320 AT4G30935.1 52.632 57 26 1 140 196 332 387 8.18e-16 77.4
Msa0054320 AT2G44745.1 52.632 57 26 1 140 196 146 201 9.96e-16 74.7
Msa0054320 AT2G30250.1 45.205 73 37 2 124 196 315 384 2.04e-15 76.3
Msa0054320 AT2G30250.1 46.479 71 34 3 140 210 167 233 2.84e-11 63.9
Msa0054320 AT3G01970.1 49.180 61 30 1 140 200 66 125 3.07e-15 71.6
Msa0054320 AT2G37260.3 45.588 68 36 1 129 196 259 325 3.88e-15 75.1
Msa0054320 AT2G37260.3 46.479 71 36 2 126 196 72 140 5.74e-15 74.3
Msa0054320 AT2G37260.2 45.588 68 36 1 129 196 257 323 4.09e-15 74.7
Msa0054320 AT2G37260.2 46.479 71 36 2 126 196 70 138 5.95e-15 74.3
Msa0054320 AT2G37260.1 45.588 68 36 1 129 196 339 405 4.24e-15 75.5
Msa0054320 AT2G37260.1 46.479 71 36 2 126 196 152 220 6.05e-15 74.7
Msa0054320 AT4G39410.1 50.877 57 27 1 140 196 224 279 5.25e-15 74.3
Msa0054320 AT1G55600.1 52.632 57 26 1 140 196 308 363 5.39e-15 75.1
Msa0054320 AT2G34830.1 47.692 65 34 0 132 196 208 272 8.44e-15 74.3
Msa0054320 AT2G34830.2 47.692 65 34 0 132 196 217 281 8.48e-15 74.3
Msa0054320 AT2G21900.1 44.444 72 38 2 126 196 95 165 8.86e-15 72.0
Msa0054320 AT1G30650.1 47.692 65 34 0 132 196 210 274 1.85e-14 73.6
Msa0054320 AT3G58710.2 49.123 57 29 0 140 196 71 127 3.37e-14 71.2
Msa0054320 AT3G58710.1 49.123 57 29 0 140 196 72 128 3.40e-14 71.2
Msa0054320 AT5G64810.1 37.805 82 46 3 116 196 89 166 4.33e-14 69.7
Msa0054320 AT2G46130.1 50.877 57 27 1 140 196 31 86 6.80e-14 67.0
Msa0054320 AT4G01250.1 45.614 57 31 0 140 196 129 185 1.22e-13 70.1
Msa0054320 AT5G43290.1 40.789 76 43 2 121 196 97 170 1.58e-13 69.7
Msa0054320 AT5G52830.1 43.478 69 39 0 140 208 166 234 1.59e-13 70.1
Msa0054320 AT4G26440.1 43.836 73 38 2 124 196 359 428 1.76e-13 70.9
Msa0054320 AT4G26440.1 47.368 57 28 2 140 196 179 233 7.48e-11 62.8
Msa0054320 AT5G45050.2 34.951 103 64 1 94 196 1110 1209 2.12e-13 70.9
Msa0054320 AT5G45050.1 34.951 103 64 1 94 196 1138 1237 2.19e-13 70.9
Msa0054320 AT5G45050.4 34.951 103 64 1 94 196 999 1098 2.21e-13 70.9
Msa0054320 AT1G69310.3 50.877 57 23 2 140 196 148 199 7.22e-13 67.8
Msa0054320 AT4G23550.1 30.714 140 88 4 138 275 139 271 1.02e-12 67.8
Msa0054320 AT2G46400.1 40.698 86 46 3 111 194 79 161 2.69e-12 66.2
Msa0054320 AT3G01080.3 49.123 57 27 2 140 196 206 260 8.70e-11 62.0
Msa0054320 AT1G80590.1 44.828 58 30 1 139 194 85 142 9.67e-11 60.8

Find 50 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTATTTCTGGAAGAATTT+TGG 0.107436 1_2:-9087178 Msa0054320:CDS
GCACTTGCCACTGCTATTTC+TGG 0.167473 1_2:-9087189 Msa0054320:CDS
TTTGGACCATACTTCTAATA+AGG 0.232805 1_2:-9087160 Msa0054320:CDS
GTCTTCACCTAAGGCTTATA+AGG 0.247602 1_2:-9088288 Msa0054320:CDS
AATTGCTTGATAGATGATTT+AGG 0.254015 1_2:+9087264 None:intergenic
TGTATAATGTTGTGCATATA+TGG 0.302379 1_2:-9087118 Msa0054320:three_prime_UTR
GCAAGTGCTGTAGTGAAATT+AGG 0.319456 1_2:+9087204 None:intergenic
ATACGAACCTTGAGTATAAA+AGG 0.320556 1_2:+9089363 None:intergenic
CAAAGTGAAGAAGCTCCTTT+AGG 0.328438 1_2:-9087357 Msa0054320:CDS
GGATATCAATGGAGGAAATA+TGG 0.328462 1_2:-9088088 Msa0054320:CDS
ATTTGGCCTTATTAGAAGTA+TGG 0.343403 1_2:+9087154 None:intergenic
AATATGTTTGGTAATGTTAG+AGG 0.347609 1_2:-9088389 Msa0054320:CDS
TACATGGAAATTTCACCATT+TGG 0.351460 1_2:+9087137 None:intergenic
TTGTGAACAGGACGAAGTTT+TGG 0.359854 1_2:-9088585 Msa0054320:intron
CCTCTCCTAGAGCTTACTTT+AGG 0.371319 1_2:-9088042 Msa0054320:CDS
TAAAAGGAACATTAACGTTA+AGG 0.404032 1_2:+9089379 None:intergenic
TATGTTGCAACTAGTATAGT+AGG 0.404321 1_2:+9087435 None:intergenic
GAAAGTTGCATCAATATGTT+TGG 0.406359 1_2:-9088401 Msa0054320:CDS
AGGAACATTATCAAGCTTCA+TGG 0.420222 1_2:+9087284 None:intergenic
ATGAAGAATCATTAATCAAA+AGG 0.454669 1_2:-9088325 Msa0054320:CDS
CTAAAGGAGCTTCTTCACTT+TGG 0.478227 1_2:+9087358 None:intergenic
ACAAGAACCTTATAAGCCTT+AGG 0.486641 1_2:+9088281 None:intergenic
CATTAATCAAAAGGCCATGT+AGG 0.493040 1_2:-9088316 Msa0054320:CDS
ATACTAGTTGCAACATATGA+AGG 0.494693 1_2:-9087429 Msa0054320:CDS
AACTGGGCAGCTTGGAGCAT+AGG 0.506437 1_2:+9088015 None:intergenic
CTTTCAGCAGCAAATTGCAA+TGG 0.512836 1_2:-9089430 None:intergenic
ATACTTCTAATAAGGCCAAA+TGG 0.514058 1_2:-9087152 Msa0054320:CDS
ACATTATCAAGCTTCATGGT+TGG 0.514996 1_2:+9087288 None:intergenic
TAGGGATATGTCTTCACCTA+AGG 0.525286 1_2:-9088297 Msa0054320:CDS
GAGCACCTAAAGTAAGCTCT+AGG 0.538868 1_2:+9088037 None:intergenic
ATTAATCAAAAGGCCATGTA+GGG 0.538909 1_2:-9088315 Msa0054320:CDS
CCTAAAGTAAGCTCTAGGAG+AGG 0.548395 1_2:+9088042 None:intergenic
GTAACATGAACTGAACCTAA+AGG 0.560432 1_2:+9087342 None:intergenic
GTCTTAGTTCTGAGAACAAG+AGG 0.567530 1_2:-9088547 Msa0054320:CDS
CTAAAGTAAGCTCTAGGAGA+GGG 0.569732 1_2:+9088043 None:intergenic
TATATGCACAACATTATACA+TGG 0.580638 1_2:+9087121 None:intergenic
TTGTGGTATAAAGTATGTGA+TGG 0.581181 1_2:-9088113 Msa0054320:intron
ATGGAGGAAATATGGTCAGA+AGG 0.585738 1_2:-9088080 Msa0054320:CDS
TTTCAGATTTCTCATGACCA+TGG 0.592416 1_2:+9087397 None:intergenic
GGTATAAAGTATGTGATGGA+TGG 0.592987 1_2:-9088109 Msa0054320:intron
ATGTGATGGATGGATATCAA+TGG 0.593080 1_2:-9088099 Msa0054320:CDS
ATGTTGCAACTAGTATAGTA+GGG 0.601976 1_2:+9087436 None:intergenic
TGCAATGGAATCAACTTGTG+TGG 0.619908 1_2:-9089415 Msa0054320:exon
GGTGAAGACATATCCCTACA+TGG 0.630918 1_2:+9088302 None:intergenic
GTTCTTTGAATAATTTGCTG+AGG 0.646817 1_2:+9087315 None:intergenic
TAAAGTAAGCTCTAGGAGAG+GGG 0.664718 1_2:+9088044 None:intergenic
TGATGGATGGATATCAATGG+AGG 0.669535 1_2:-9088096 Msa0054320:CDS
AATTCTTCCAGAAATAGCAG+TGG 0.680346 1_2:+9087182 None:intergenic
TATGAAGGAGAGCACAACCA+TGG 0.694590 1_2:-9087414 Msa0054320:CDS
TAACATGTGTGAGAACTGTG+AGG 0.784888 1_2:-9088507 Msa0054320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTAATTCATAATCA+AGG - chr1_2:9087301-9087320 Msa0054320:CDS 10.0%
!! TATACTAATTTCATTAAAAT+TGG - chr1_2:9087742-9087761 Msa0054320:intron 10.0%
!! AAAATATTAATTCATAATCA+AGG - chr1_2:9087301-9087320 Msa0054320:CDS 10.0%
!! TATACTAATTTCATTAAAAT+TGG - chr1_2:9087742-9087761 Msa0054320:intron 10.0%
!! ATATATTAGCGAATATATTA+GGG + chr1_2:9087570-9087589 None:intergenic 15.0%
!! AATATATTAGCGAATATATT+AGG + chr1_2:9087571-9087590 None:intergenic 15.0%
!! ATATATTAGCGAATATATTA+GGG + chr1_2:9087570-9087589 None:intergenic 15.0%
!! AATATATTAGCGAATATATT+AGG + chr1_2:9087571-9087590 None:intergenic 15.0%
!!! TTTAATTTTCATGTATGATC+AGG - chr1_2:9087112-9087131 Msa0054320:three_prime_UTR 20.0%
!!! TTAATTTTCATGTATGATCA+GGG - chr1_2:9087113-9087132 Msa0054320:three_prime_UTR 20.0%
!!! TTGATAATGTAAACCTAAAA+AGG - chr1_2:9087495-9087514 Msa0054320:intron 20.0%
!! ATGAAGAATCATTAATCAAA+AGG - chr1_2:9088099-9088118 Msa0054320:CDS 20.0%
!!! TTATTACCTTCTTTTTAACT+GGG + chr1_2:9088428-9088447 None:intergenic 20.0%
!!! ATTATTACCTTCTTTTTAAC+TGG + chr1_2:9088429-9088448 None:intergenic 20.0%
!! AAAAATTTGACAAGAACTTT+CGG - chr1_2:9088558-9088577 Msa0054320:CDS 20.0%
!! TTTATATGAAAACATGTGAT+TGG + chr1_2:9088597-9088616 None:intergenic 20.0%
!! ATTCTTTCTCATCAATATAT+TGG + chr1_2:9088670-9088689 None:intergenic 20.0%
!!! TATTGATGAGAAAGAATTTT+AGG - chr1_2:9088673-9088692 Msa0054320:intron 20.0%
!!! ATTGATGAGAAAGAATTTTA+GGG - chr1_2:9088674-9088693 Msa0054320:intron 20.0%
!! CGTTGAAAAGAAAAAATAAA+GGG + chr1_2:9088732-9088751 None:intergenic 20.0%
!! TCGTTGAAAAGAAAAAATAA+AGG + chr1_2:9088733-9088752 None:intergenic 20.0%
!!! ATCAAAACTTGATCTTATTT+GGG + chr1_2:9088926-9088945 None:intergenic 20.0%
!! CTCTTATGTCATTATTAATT+AGG - chr1_2:9088946-9088965 Msa0054320:intron 20.0%
!!! TTTAATTTTCATGTATGATC+AGG - chr1_2:9087112-9087131 Msa0054320:three_prime_UTR 20.0%
!!! TTAATTTTCATGTATGATCA+GGG - chr1_2:9087113-9087132 Msa0054320:three_prime_UTR 20.0%
!!! TTGATAATGTAAACCTAAAA+AGG - chr1_2:9087495-9087514 Msa0054320:intron 20.0%
!! ATGAAGAATCATTAATCAAA+AGG - chr1_2:9088099-9088118 Msa0054320:CDS 20.0%
!!! TTATTACCTTCTTTTTAACT+GGG + chr1_2:9088428-9088447 None:intergenic 20.0%
!!! ATTATTACCTTCTTTTTAAC+TGG + chr1_2:9088429-9088448 None:intergenic 20.0%
!! AAAAATTTGACAAGAACTTT+CGG - chr1_2:9088558-9088577 Msa0054320:CDS 20.0%
!! TTTATATGAAAACATGTGAT+TGG + chr1_2:9088597-9088616 None:intergenic 20.0%
!! ATTCTTTCTCATCAATATAT+TGG + chr1_2:9088670-9088689 None:intergenic 20.0%
!!! TATTGATGAGAAAGAATTTT+AGG - chr1_2:9088673-9088692 Msa0054320:intron 20.0%
!!! ATTGATGAGAAAGAATTTTA+GGG - chr1_2:9088674-9088693 Msa0054320:intron 20.0%
!! CGTTGAAAAGAAAAAATAAA+GGG + chr1_2:9088732-9088751 None:intergenic 20.0%
!! TCGTTGAAAAGAAAAAATAA+AGG + chr1_2:9088733-9088752 None:intergenic 20.0%
!!! ATCAAAACTTGATCTTATTT+GGG + chr1_2:9088926-9088945 None:intergenic 20.0%
!! CTCTTATGTCATTATTAATT+AGG - chr1_2:9088946-9088965 Msa0054320:intron 20.0%
! TAAAAGGAACATTAACGTTA+AGG + chr1_2:9087048-9087067 None:intergenic 25.0%
!! TAATGTTCCTTTTATACTCA+AGG - chr1_2:9087054-9087073 Msa0054320:three_prime_UTR 25.0%
! ACTTAAGAAAGTAGTTAACA+AGG - chr1_2:9087183-9087202 Msa0054320:CDS 25.0%
! TCAATCAATCTTAACTACTA+TGG + chr1_2:9087479-9087498 None:intergenic 25.0%
! CACTATATATACACCTAAAA+AGG - chr1_2:9087645-9087664 Msa0054320:intron 25.0%
!!! AATATGTTTGGTAATGTTAG+AGG - chr1_2:9088035-9088054 Msa0054320:CDS 25.0%
! ATGTTAAGAATACACATGTT+TGG + chr1_2:9088488-9088507 None:intergenic 25.0%
! TTTCGATAGAGATTACTAAT+AGG + chr1_2:9088541-9088560 None:intergenic 25.0%
!! GTAAACAAATGCTAAGATTT+TGG - chr1_2:9088876-9088895 Msa0054320:intron 25.0%
! GATCAAAACTTGATCTTATT+TGG + chr1_2:9088927-9088946 None:intergenic 25.0%
! AATTGCTTGATAGATGATTT+AGG + chr1_2:9089163-9089182 None:intergenic 25.0%
!! ATCATCTATCAAGCAATTTT+TGG - chr1_2:9089165-9089184 Msa0054320:intron 25.0%
! TATATGCACAACATTATACA+TGG + chr1_2:9089306-9089325 None:intergenic 25.0%
! TGTATAATGTTGTGCATATA+TGG - chr1_2:9089306-9089325 Msa0054320:intron 25.0%
! TAAAAGGAACATTAACGTTA+AGG + chr1_2:9087048-9087067 None:intergenic 25.0%
!! TAATGTTCCTTTTATACTCA+AGG - chr1_2:9087054-9087073 Msa0054320:three_prime_UTR 25.0%
! ACTTAAGAAAGTAGTTAACA+AGG - chr1_2:9087183-9087202 Msa0054320:CDS 25.0%
! TCAATCAATCTTAACTACTA+TGG + chr1_2:9087479-9087498 None:intergenic 25.0%
! CACTATATATACACCTAAAA+AGG - chr1_2:9087645-9087664 Msa0054320:intron 25.0%
!!! AATATGTTTGGTAATGTTAG+AGG - chr1_2:9088035-9088054 Msa0054320:CDS 25.0%
! ATGTTAAGAATACACATGTT+TGG + chr1_2:9088488-9088507 None:intergenic 25.0%
! TTTCGATAGAGATTACTAAT+AGG + chr1_2:9088541-9088560 None:intergenic 25.0%
!! GTAAACAAATGCTAAGATTT+TGG - chr1_2:9088876-9088895 Msa0054320:intron 25.0%
! GATCAAAACTTGATCTTATT+TGG + chr1_2:9088927-9088946 None:intergenic 25.0%
! AATTGCTTGATAGATGATTT+AGG + chr1_2:9089163-9089182 None:intergenic 25.0%
!! ATCATCTATCAAGCAATTTT+TGG - chr1_2:9089165-9089184 Msa0054320:intron 25.0%
! TATATGCACAACATTATACA+TGG + chr1_2:9089306-9089325 None:intergenic 25.0%
! TGTATAATGTTGTGCATATA+TGG - chr1_2:9089306-9089325 Msa0054320:intron 25.0%
ATACGAACCTTGAGTATAAA+AGG + chr1_2:9087064-9087083 None:intergenic 30.0%
AAGAAAGTAGTTAACAAGGT+TGG - chr1_2:9087187-9087206 Msa0054320:CDS 30.0%
!!! CTTAGGGCACAAATTTTTTT+GGG + chr1_2:9087424-9087443 None:intergenic 30.0%
!!! ACTTAGGGCACAAATTTTTT+TGG + chr1_2:9087425-9087444 None:intergenic 30.0%
TCAATCTTAACTACTATGGA+TGG + chr1_2:9087475-9087494 None:intergenic 30.0%
! ATCGCTAATTCAACCTTTTT+AGG + chr1_2:9087511-9087530 None:intergenic 30.0%
AACCTAAAAATTCAACCTCA+AGG - chr1_2:9087533-9087552 Msa0054320:intron 30.0%
! AACCTTGAGGTTGAATTTTT+AGG + chr1_2:9087538-9087557 None:intergenic 30.0%
! ATCGCTAATTCAACCTTTTT+AGG + chr1_2:9087511-9087530 None:intergenic 30.0%
! CAATGTCACTTAGAAATTCA+GGG + chr1_2:9087813-9087832 None:intergenic 30.0%
! ACATTGGAAATTTTGTGAAC+AGG - chr1_2:9087827-9087846 Msa0054320:intron 30.0%
GAAAGTTGCATCAATATGTT+TGG - chr1_2:9088023-9088042 Msa0054320:CDS 30.0%
ATTAATCAAAAGGCCATGTA+GGG - chr1_2:9088109-9088128 Msa0054320:intron 30.0%
TTGTGGTATAAAGTATGTGA+TGG - chr1_2:9088311-9088330 Msa0054320:CDS 30.0%
AGATTACTAATAGGTGAAGT+AGG + chr1_2:9088532-9088551 None:intergenic 30.0%
TATGAAAACATGTGATTGGT+AGG + chr1_2:9088593-9088612 None:intergenic 30.0%
ATGTTGCAACTAGTATAGTA+GGG + chr1_2:9088991-9089010 None:intergenic 30.0%
TATGTTGCAACTAGTATAGT+AGG + chr1_2:9088992-9089011 None:intergenic 30.0%
ATACTAGTTGCAACATATGA+AGG - chr1_2:9088995-9089014 Msa0054320:intron 30.0%
!! GTTCTTTGAATAATTTGCTG+AGG + chr1_2:9089112-9089131 None:intergenic 30.0%
!!! GCAATTTTTGGTTCAACAAA+TGG - chr1_2:9089177-9089196 Msa0054320:intron 30.0%
!!! TGCTATTTCTGGAAGAATTT+TGG - chr1_2:9089246-9089265 Msa0054320:intron 30.0%
TTTGGACCATACTTCTAATA+AGG - chr1_2:9089264-9089283 Msa0054320:intron 30.0%
!! ATTTGGCCTTATTAGAAGTA+TGG + chr1_2:9089273-9089292 None:intergenic 30.0%
ATACTTCTAATAAGGCCAAA+TGG - chr1_2:9089272-9089291 Msa0054320:intron 30.0%
TACATGGAAATTTCACCATT+TGG + chr1_2:9089290-9089309 None:intergenic 30.0%
ATACGAACCTTGAGTATAAA+AGG + chr1_2:9087064-9087083 None:intergenic 30.0%
AAGAAAGTAGTTAACAAGGT+TGG - chr1_2:9087187-9087206 Msa0054320:CDS 30.0%
!!! CTTAGGGCACAAATTTTTTT+GGG + chr1_2:9087424-9087443 None:intergenic 30.0%
!!! ACTTAGGGCACAAATTTTTT+TGG + chr1_2:9087425-9087444 None:intergenic 30.0%
TCAATCTTAACTACTATGGA+TGG + chr1_2:9087475-9087494 None:intergenic 30.0%
! ATCGCTAATTCAACCTTTTT+AGG + chr1_2:9087511-9087530 None:intergenic 30.0%
AACCTAAAAATTCAACCTCA+AGG - chr1_2:9087533-9087552 Msa0054320:intron 30.0%
! AACCTTGAGGTTGAATTTTT+AGG + chr1_2:9087538-9087557 None:intergenic 30.0%
! ATCGCTAATTCAACCTTTTT+AGG + chr1_2:9087511-9087530 None:intergenic 30.0%
! CAATGTCACTTAGAAATTCA+GGG + chr1_2:9087813-9087832 None:intergenic 30.0%
! ACATTGGAAATTTTGTGAAC+AGG - chr1_2:9087827-9087846 Msa0054320:intron 30.0%
GAAAGTTGCATCAATATGTT+TGG - chr1_2:9088023-9088042 Msa0054320:CDS 30.0%
ATTAATCAAAAGGCCATGTA+GGG - chr1_2:9088109-9088128 Msa0054320:intron 30.0%
TTGTGGTATAAAGTATGTGA+TGG - chr1_2:9088311-9088330 Msa0054320:CDS 30.0%
AGATTACTAATAGGTGAAGT+AGG + chr1_2:9088532-9088551 None:intergenic 30.0%
TATGAAAACATGTGATTGGT+AGG + chr1_2:9088593-9088612 None:intergenic 30.0%
ATGTTGCAACTAGTATAGTA+GGG + chr1_2:9088991-9089010 None:intergenic 30.0%
TATGTTGCAACTAGTATAGT+AGG + chr1_2:9088992-9089011 None:intergenic 30.0%
ATACTAGTTGCAACATATGA+AGG - chr1_2:9088995-9089014 Msa0054320:intron 30.0%
!! GTTCTTTGAATAATTTGCTG+AGG + chr1_2:9089112-9089131 None:intergenic 30.0%
!!! GCAATTTTTGGTTCAACAAA+TGG - chr1_2:9089177-9089196 Msa0054320:intron 30.0%
!!! TGCTATTTCTGGAAGAATTT+TGG - chr1_2:9089246-9089265 Msa0054320:intron 30.0%
TTTGGACCATACTTCTAATA+AGG - chr1_2:9089264-9089283 Msa0054320:intron 30.0%
!! ATTTGGCCTTATTAGAAGTA+TGG + chr1_2:9089273-9089292 None:intergenic 30.0%
ATACTTCTAATAAGGCCAAA+TGG - chr1_2:9089272-9089291 Msa0054320:intron 30.0%
TACATGGAAATTTCACCATT+TGG + chr1_2:9089290-9089309 None:intergenic 30.0%
! CCAATGTCACTTAGAAATTC+AGG + chr1_2:9087814-9087833 None:intergenic 35.0%
CCTGAATTTCTAAGTGACAT+TGG - chr1_2:9087811-9087830 Msa0054320:intron 35.0%
CATTAATCAAAAGGCCATGT+AGG - chr1_2:9088108-9088127 Msa0054320:intron 35.0%
ACAAGAACCTTATAAGCCTT+AGG + chr1_2:9088146-9088165 None:intergenic 35.0%
ACACACATTATTGAAGTTGC+TGG + chr1_2:9088241-9088260 None:intergenic 35.0%
! GGTATAAAGTATGTGATGGA+TGG - chr1_2:9088315-9088334 Msa0054320:CDS 35.0%
!! ATGTGATGGATGGATATCAA+TGG - chr1_2:9088325-9088344 Msa0054320:CDS 35.0%
GGATATCAATGGAGGAAATA+TGG - chr1_2:9088336-9088355 Msa0054320:CDS 35.0%
TTACTAATAGGTGAAGTAGG+CGG + chr1_2:9088529-9088548 None:intergenic 35.0%
AATTTGACAAGAACTTTCGG+TGG - chr1_2:9088561-9088580 Msa0054320:CDS 35.0%
ATTTGACAAGAACTTTCGGT+GGG - chr1_2:9088562-9088581 Msa0054320:CDS 35.0%
! CATCAATATATTGGTCTGAG+TGG + chr1_2:9088661-9088680 None:intergenic 35.0%
TTTCAGATTTCTCATGACCA+TGG + chr1_2:9089030-9089049 None:intergenic 35.0%
GTAACATGAACTGAACCTAA+AGG + chr1_2:9089085-9089104 None:intergenic 35.0%
ACATTATCAAGCTTCATGGT+TGG + chr1_2:9089139-9089158 None:intergenic 35.0%
AGGAACATTATCAAGCTTCA+TGG + chr1_2:9089143-9089162 None:intergenic 35.0%
AATTCTTCCAGAAATAGCAG+TGG + chr1_2:9089245-9089264 None:intergenic 35.0%
! CCAATGTCACTTAGAAATTC+AGG + chr1_2:9087814-9087833 None:intergenic 35.0%
CCTGAATTTCTAAGTGACAT+TGG - chr1_2:9087811-9087830 Msa0054320:intron 35.0%
CATTAATCAAAAGGCCATGT+AGG - chr1_2:9088108-9088127 Msa0054320:intron 35.0%
ACAAGAACCTTATAAGCCTT+AGG + chr1_2:9088146-9088165 None:intergenic 35.0%
ACACACATTATTGAAGTTGC+TGG + chr1_2:9088241-9088260 None:intergenic 35.0%
! GGTATAAAGTATGTGATGGA+TGG - chr1_2:9088315-9088334 Msa0054320:CDS 35.0%
!! ATGTGATGGATGGATATCAA+TGG - chr1_2:9088325-9088344 Msa0054320:CDS 35.0%
GGATATCAATGGAGGAAATA+TGG - chr1_2:9088336-9088355 Msa0054320:CDS 35.0%
TTACTAATAGGTGAAGTAGG+CGG + chr1_2:9088529-9088548 None:intergenic 35.0%
AATTTGACAAGAACTTTCGG+TGG - chr1_2:9088561-9088580 Msa0054320:CDS 35.0%
ATTTGACAAGAACTTTCGGT+GGG - chr1_2:9088562-9088581 Msa0054320:CDS 35.0%
! CATCAATATATTGGTCTGAG+TGG + chr1_2:9088661-9088680 None:intergenic 35.0%
TTTCAGATTTCTCATGACCA+TGG + chr1_2:9089030-9089049 None:intergenic 35.0%
GTAACATGAACTGAACCTAA+AGG + chr1_2:9089085-9089104 None:intergenic 35.0%
ACATTATCAAGCTTCATGGT+TGG + chr1_2:9089139-9089158 None:intergenic 35.0%
AGGAACATTATCAAGCTTCA+TGG + chr1_2:9089143-9089162 None:intergenic 35.0%
AATTCTTCCAGAAATAGCAG+TGG + chr1_2:9089245-9089264 None:intergenic 35.0%
TGCAATGGAATCAACTTGTG+TGG - chr1_2:9087009-9087028 Msa0054320:three_prime_UTR 40.0%
GAACGTGAGTGATGAATTGA+CGG + chr1_2:9087152-9087171 None:intergenic 40.0%
! TTGTGAACAGGACGAAGTTT+TGG - chr1_2:9087839-9087858 Msa0054320:intron 40.0%
!! GTCTTAGTTCTGAGAACAAG+AGG - chr1_2:9087877-9087896 Msa0054320:intron 40.0%
TAACATGTGTGAGAACTGTG+AGG - chr1_2:9087917-9087936 Msa0054320:intron 40.0%
TAGGGATATGTCTTCACCTA+AGG - chr1_2:9088127-9088146 Msa0054320:intron 40.0%
GTCTTCACCTAAGGCTTATA+AGG - chr1_2:9088136-9088155 Msa0054320:intron 40.0%
! TGATGGATGGATATCAATGG+AGG - chr1_2:9088328-9088347 Msa0054320:CDS 40.0%
ATGGAGGAAATATGGTCAGA+AGG - chr1_2:9088344-9088363 Msa0054320:CDS 40.0%
!! TAAAGTAAGCTCTAGGAGAG+GGG + chr1_2:9088383-9088402 None:intergenic 40.0%
!! CTAAAGTAAGCTCTAGGAGA+GGG + chr1_2:9088384-9088403 None:intergenic 40.0%
!! TTCTTTTTAACTGGGCAGCT+TGG + chr1_2:9088420-9088439 None:intergenic 40.0%
AAGCTGCCCAGTTAAAAAGA+AGG - chr1_2:9088419-9088438 Msa0054320:CDS 40.0%
CTAAAGGAGCTTCTTCACTT+TGG + chr1_2:9089069-9089088 None:intergenic 40.0%
CAAAGTGAAGAAGCTCCTTT+AGG - chr1_2:9089067-9089086 Msa0054320:intron 40.0%
! GCAAGTGCTGTAGTGAAATT+AGG + chr1_2:9089223-9089242 None:intergenic 40.0%
TGCAATGGAATCAACTTGTG+TGG - chr1_2:9087009-9087028 Msa0054320:three_prime_UTR 40.0%
GAACGTGAGTGATGAATTGA+CGG + chr1_2:9087152-9087171 None:intergenic 40.0%
! TTGTGAACAGGACGAAGTTT+TGG - chr1_2:9087839-9087858 Msa0054320:intron 40.0%
!! GTCTTAGTTCTGAGAACAAG+AGG - chr1_2:9087877-9087896 Msa0054320:intron 40.0%
TAACATGTGTGAGAACTGTG+AGG - chr1_2:9087917-9087936 Msa0054320:intron 40.0%
TAGGGATATGTCTTCACCTA+AGG - chr1_2:9088127-9088146 Msa0054320:intron 40.0%
GTCTTCACCTAAGGCTTATA+AGG - chr1_2:9088136-9088155 Msa0054320:intron 40.0%
! TGATGGATGGATATCAATGG+AGG - chr1_2:9088328-9088347 Msa0054320:CDS 40.0%
ATGGAGGAAATATGGTCAGA+AGG - chr1_2:9088344-9088363 Msa0054320:CDS 40.0%
!! TAAAGTAAGCTCTAGGAGAG+GGG + chr1_2:9088383-9088402 None:intergenic 40.0%
!! CTAAAGTAAGCTCTAGGAGA+GGG + chr1_2:9088384-9088403 None:intergenic 40.0%
!! TTCTTTTTAACTGGGCAGCT+TGG + chr1_2:9088420-9088439 None:intergenic 40.0%
AAGCTGCCCAGTTAAAAAGA+AGG - chr1_2:9088419-9088438 Msa0054320:CDS 40.0%
CTAAAGGAGCTTCTTCACTT+TGG + chr1_2:9089069-9089088 None:intergenic 40.0%
CAAAGTGAAGAAGCTCCTTT+AGG - chr1_2:9089067-9089086 Msa0054320:intron 40.0%
! GCAAGTGCTGTAGTGAAATT+AGG + chr1_2:9089223-9089242 None:intergenic 40.0%
! AACTACTATGGATGGCCTTG+AGG + chr1_2:9087467-9087486 None:intergenic 45.0%
AGGGCACAAATTCAACCTTG+AGG + chr1_2:9087551-9087570 None:intergenic 45.0%
GGTGAAGACATATCCCTACA+TGG + chr1_2:9088125-9088144 None:intergenic 45.0%
!! CCTAAAGTAAGCTCTAGGAG+AGG + chr1_2:9088385-9088404 None:intergenic 45.0%
CCTCTCCTAGAGCTTACTTT+AGG - chr1_2:9088382-9088401 Msa0054320:CDS 45.0%
GAGCACCTAAAGTAAGCTCT+AGG + chr1_2:9088390-9088409 None:intergenic 45.0%
! TATGAAGGAGAGCACAACCA+TGG - chr1_2:9089010-9089029 Msa0054320:intron 45.0%
! AACTACTATGGATGGCCTTG+AGG + chr1_2:9087467-9087486 None:intergenic 45.0%
AGGGCACAAATTCAACCTTG+AGG + chr1_2:9087551-9087570 None:intergenic 45.0%
GGTGAAGACATATCCCTACA+TGG + chr1_2:9088125-9088144 None:intergenic 45.0%
!! CCTAAAGTAAGCTCTAGGAG+AGG + chr1_2:9088385-9088404 None:intergenic 45.0%
CCTCTCCTAGAGCTTACTTT+AGG - chr1_2:9088382-9088401 Msa0054320:CDS 45.0%
GAGCACCTAAAGTAAGCTCT+AGG + chr1_2:9088390-9088409 None:intergenic 45.0%
! TATGAAGGAGAGCACAACCA+TGG - chr1_2:9089010-9089029 Msa0054320:intron 45.0%
!!! TAAAAATTTAAAATTAATCA+AGG + chr1_2:9088290-9088309 None:intergenic 5.0%
!!! TAATTTTAAATTTTTATTTG+TGG - chr1_2:9088294-9088313 Msa0054320:CDS 5.0%
!!! TAAAAATTTAAAATTAATCA+AGG + chr1_2:9088290-9088309 None:intergenic 5.0%
!!! TAATTTTAAATTTTTATTTG+TGG - chr1_2:9088294-9088313 Msa0054320:CDS 5.0%
! CTATGGATGGCCTTGAGGTT+GGG + chr1_2:9087462-9087481 None:intergenic 50.0%
! ACTATGGATGGCCTTGAGGT+TGG + chr1_2:9087463-9087482 None:intergenic 50.0%
!! GCACTTGCCACTGCTATTTC+TGG - chr1_2:9089235-9089254 Msa0054320:intron 50.0%
! CTATGGATGGCCTTGAGGTT+GGG + chr1_2:9087462-9087481 None:intergenic 50.0%
! ACTATGGATGGCCTTGAGGT+TGG + chr1_2:9087463-9087482 None:intergenic 50.0%
!! GCACTTGCCACTGCTATTTC+TGG - chr1_2:9089235-9089254 Msa0054320:intron 50.0%
AACTGGGCAGCTTGGAGCAT+AGG + chr1_2:9088412-9088431 None:intergenic 55.0%
AACTGGGCAGCTTGGAGCAT+AGG + chr1_2:9088412-9088431 None:intergenic 55.0%
GCAGGAGGGGCGGAAACTTA+GGG + chr1_2:9087440-9087459 None:intergenic 60.0%
GCAGGAGGGGCGGAAACTTA+GGG + chr1_2:9087440-9087459 None:intergenic 60.0%
GGCAGGAGGGGCGGAAACTT+AGG + chr1_2:9087441-9087460 None:intergenic 65.0%
TTGAGGTTGGGCAGGAGGGG+CGG + chr1_2:9087450-9087469 None:intergenic 65.0%
GCCTTGAGGTTGGGCAGGAG+GGG + chr1_2:9087453-9087472 None:intergenic 65.0%
GGCCTTGAGGTTGGGCAGGA+GGG + chr1_2:9087454-9087473 None:intergenic 65.0%
! TGGCCTTGAGGTTGGGCAGG+AGG + chr1_2:9087455-9087474 None:intergenic 65.0%
! GGATGGCCTTGAGGTTGGGC+AGG + chr1_2:9087458-9087477 None:intergenic 65.0%
GGCAGGAGGGGCGGAAACTT+AGG + chr1_2:9087441-9087460 None:intergenic 65.0%
TTGAGGTTGGGCAGGAGGGG+CGG + chr1_2:9087450-9087469 None:intergenic 65.0%
GCCTTGAGGTTGGGCAGGAG+GGG + chr1_2:9087453-9087472 None:intergenic 65.0%
GGCCTTGAGGTTGGGCAGGA+GGG + chr1_2:9087454-9087473 None:intergenic 65.0%
! TGGCCTTGAGGTTGGGCAGG+AGG + chr1_2:9087455-9087474 None:intergenic 65.0%
! GGATGGCCTTGAGGTTGGGC+AGG + chr1_2:9087458-9087477 None:intergenic 65.0%
GCCCCTCCTGCCCAACCTCA+AGG - chr1_2:9087449-9087468 Msa0054320:CDS 70.0%
GCCCCTCCTGCCCAACCTCA+AGG - chr1_2:9087449-9087468 Msa0054320:CDS 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9087003 9089443 9087003 ID=Msa0054320;Name=Msa0054320
chr1_2 mRNA 9087003 9089443 9087003 ID=Msa0054320-mRNA-1;Parent=Msa0054320;Name=Msa0054320-mRNA-1;_AED=0.32;_eAED=0.32;_QI=10|1|1|1|1|1|4|146|284
chr1_2 exon 9087003 9087478 9087003 ID=Msa0054320-mRNA-1:exon:3321;Parent=Msa0054320-mRNA-1
chr1_2 exon 9088006 9088122 9088006 ID=Msa0054320-mRNA-1:exon:3320;Parent=Msa0054320-mRNA-1
chr1_2 exon 9088253 9088597 9088253 ID=Msa0054320-mRNA-1:exon:3319;Parent=Msa0054320-mRNA-1
chr1_2 exon 9089371 9089443 9089371 ID=Msa0054320-mRNA-1:exon:3318;Parent=Msa0054320-mRNA-1
chr1_2 five_prime_UTR 9089434 9089443 9089434 ID=Msa0054320-mRNA-1:five_prime_utr;Parent=Msa0054320-mRNA-1
chr1_2 CDS 9089371 9089433 9089371 ID=Msa0054320-mRNA-1:cds;Parent=Msa0054320-mRNA-1
chr1_2 CDS 9088253 9088597 9088253 ID=Msa0054320-mRNA-1:cds;Parent=Msa0054320-mRNA-1
chr1_2 CDS 9088006 9088122 9088006 ID=Msa0054320-mRNA-1:cds;Parent=Msa0054320-mRNA-1
chr1_2 CDS 9087149 9087478 9087149 ID=Msa0054320-mRNA-1:cds;Parent=Msa0054320-mRNA-1
chr1_2 three_prime_UTR 9087003 9087148 9087003 ID=Msa0054320-mRNA-1:three_prime_utr;Parent=Msa0054320-mRNA-1
Gene Sequence

>Msa0054320

ATGGAATCAACTTGTGTGGATACTTCACTTAACCTTAACGTTAATGTTCCTTTTATACTCAAGGACGAAGTTTTGGTTGAAGAGTTACATCGTCTTAGTTCTGAGAACAAGAGGCTAAATGAAACATTGACTAACATGTGTGAGAACTGTGAGGCTATGCAAAAGCAATTGAATCAATTGATGAATCAAAATTTTGAAAATCAAACACAACAATCAAAGAAAAGAAAAGCTGAGAGTGAAAGTTGCATCAATATGTTTGGTAATGTTAGAGGCATTAATATTATCAATAATAATGAGTGCAGCACTGTTACTGATGAAGAATCATTAATCAAAAGGCCATGTAGGGATATGTCTTCACCTAAGGCTTATAAGGTTCTTGTGAAGACAGAAGCATCTAGTAATAGCTTATATGTGATGGATGGATATCAATGGAGGAAATATGGTCAGAAGGTTACTAGAGATAACCCCTCTCCTAGAGCTTACTTTAGGTGCTCCTATGCTCCAAGCTGCCCAGTTAAAAAGAAGGTGCAAAAAAGTGTAGAAGACCCTACTATACTAGTTGCAACATATGAAGGAGAGCACAACCATGGTCATGAGAAATCTGAAATATCAATGATATCAAGCCAAAGTGAAGAAGCTCCTTTAGGTTCAGTTCATGTTACTTCACCTCAGCAAATTATTCAAAGAACATGTCCAACCATGAAGCTTGATAATGTTCCTAAATCATCTATCAAGCAATTTTTGGTTCAACAAATGGCTACTTCTTTAACAAATGATCCTAATTTCACTACAGCACTTGCCACTGCTATTTCTGGAAGAATTTTGGACCATACTTCTAATAAGGCCAAATGGTGA
Protein sequence

>Msa0054320

MESTCVDTSLNLNVNVPFILKDEVLVEELHRLSSENKRLNETLTNMCENCEAMQKQLNQLMNQNFENQTQQSKKRKAESESCINMFGNVRGINIINNNECSTVTDEESLIKRPCRDMSSPKAYKVLVKTEASSNSLYVMDGYQWRKYGQKVTRDNPSPRAYFRCSYAPSCPVKKKVQKSVEDPTILVATYEGEHNHGHEKSEISMISSQSEEAPLGSVHVTSPQQIIQRTCPTMKLDNVPKSSIKQFLVQQMATSLTNDPNFTTALATAISGRILDHTSNKAKW*