AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0054330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054330 MtrunA17_Chr1g0150281 96.250 240 8 1 1 239 46 285 4.67e-176 485
Msa0054330 MtrunA17_Chr3g0136961 57.959 245 79 8 1 230 51 286 2.50e-81 245
Msa0054330 MtrunA17_Chr2g0333851 45.382 249 102 5 1 230 80 313 3.64e-58 187
Msa0054330 MtrunA17_Chr4g0001361 49.419 172 69 4 76 230 144 314 1.83e-45 154
Msa0054330 MtrunA17_Chr1g0150271 38.306 248 122 6 1 231 5 238 1.99e-42 144
Msa0054330 MtrunA17_Chr7g0270831 31.336 217 97 4 63 229 187 401 4.72e-26 105
Msa0054330 MtrunA17_Chr3g0099641 33.010 206 80 5 79 226 236 441 8.10e-25 102
Msa0054330 MtrunA17_Chr4g0051181 52.564 78 37 0 74 151 223 300 5.31e-24 99.8
Msa0054330 MtrunA17_Chr4g0060271 56.000 75 33 0 77 151 238 312 9.48e-24 99.4
Msa0054330 MtrunA17_Chr2g0288871 57.534 73 31 0 79 151 290 362 1.74e-23 98.6
Msa0054330 MtrunA17_Chr2g0322391 48.889 90 46 0 79 168 334 423 1.94e-23 98.6
Msa0054330 MtrunA17_Chr7g0216891 53.333 75 35 0 77 151 202 276 2.36e-23 98.2
Msa0054330 MtrunA17_Chr2g0302391 41.739 115 56 3 46 151 156 268 6.74e-23 96.3
Msa0054330 MtrunA17_Chr4g0075201 50.000 82 41 0 79 160 252 333 3.50e-22 94.7
Msa0054330 MtrunA17_Chr4g0025061 36.184 152 82 4 20 170 299 436 9.90e-21 90.5
Msa0054330 MtrunA17_Chr4g0025051 48.148 81 42 0 71 151 312 392 1.30e-20 90.1
Msa0054330 MtrunA17_Chr3g0101131 39.286 112 59 4 46 151 363 471 1.12e-19 87.4
Msa0054330 MtrunA17_Chr7g0270191 49.351 77 36 2 75 151 128 201 1.43e-19 85.5
Msa0054330 MtrunA17_Chr5g0445311 57.895 57 23 1 95 151 365 420 1.59e-19 87.0
Msa0054330 MtrunA17_Chr5g0445311 34.862 109 62 3 50 151 149 255 5.51e-11 62.0
Msa0054330 MtrunA17_Chr1g0194481 48.649 74 33 2 78 151 146 214 1.82e-19 85.5
Msa0054330 MtrunA17_Chr5g0447701 52.703 74 32 2 79 152 239 309 1.92e-19 86.3
Msa0054330 MtrunA17_Chr5g0401821 54.386 57 26 0 95 151 200 256 1.94e-19 84.7
Msa0054330 MtrunA17_Chr5g0443131 47.297 74 34 2 78 151 145 213 3.61e-19 84.3
Msa0054330 MtrunA17_Chr5g0418781 42.308 104 48 4 54 151 83 180 3.65e-19 84.0
Msa0054330 MtrunA17_Chr4g0053281 50.769 65 32 0 95 159 267 331 3.76e-19 84.7
Msa0054330 MtrunA17_Chr4g0051881 57.895 57 23 1 95 151 179 234 4.23e-19 84.7
Msa0054330 MtrunA17_Chr1g0184691 49.315 73 35 1 82 152 279 351 4.95e-19 84.7
Msa0054330 MtrunA17_Chr1g0147881 36.538 104 55 1 48 151 227 319 1.25e-18 83.6
Msa0054330 MtrunA17_Chr5g0432161 41.935 93 47 4 60 151 391 477 2.19e-18 83.6
Msa0054330 MtrunA17_Chr5g0432161 50.877 57 26 2 95 151 245 299 1.15e-13 69.7
Msa0054330 MtrunA17_Chr7g0263531 50.746 67 32 1 85 151 352 417 2.94e-18 83.2
Msa0054330 MtrunA17_Chr7g0263531 58.621 58 22 2 95 152 190 245 1.55e-16 78.2
Msa0054330 MtrunA17_Chr2g0294231 47.297 74 34 2 78 151 191 259 3.36e-18 82.4
Msa0054330 MtrunA17_Chr5g0418191 45.122 82 39 3 70 151 301 376 3.54e-18 82.8
Msa0054330 MtrunA17_Chr5g0418191 54.386 57 24 2 95 151 152 206 3.56e-14 71.2
Msa0054330 MtrunA17_Chr4g0042831 46.341 82 38 3 70 151 259 334 3.59e-18 82.4
Msa0054330 MtrunA17_Chr4g0042831 47.368 57 29 1 95 151 146 201 6.69e-11 61.6
Msa0054330 MtrunA17_Chr7g0218081 34.677 124 75 3 28 151 349 466 5.85e-18 82.4
Msa0054330 MtrunA17_Chr7g0218081 49.123 57 27 2 95 151 229 283 1.85e-11 63.2
Msa0054330 MtrunA17_Chr7g0228941 52.632 57 27 0 95 151 53 109 6.22e-18 77.0
Msa0054330 MtrunA17_Chr1g0188941 50.877 57 27 1 95 151 52 107 1.13e-17 76.3
Msa0054330 MtrunA17_Chr7g0238921 49.333 75 37 1 95 169 125 198 3.53e-17 79.0
Msa0054330 MtrunA17_Chr3g0106491 46.575 73 36 2 79 151 352 421 3.53e-17 80.1
Msa0054330 MtrunA17_Chr3g0106491 52.632 57 25 2 95 151 199 253 7.67e-14 70.5
Msa0054330 MtrunA17_Chr8g0379461 51.724 58 28 0 95 152 77 134 6.59e-17 77.4
Msa0054330 MtrunA17_Chr4g0004911 41.304 92 48 3 60 151 307 392 8.61e-17 79.0
Msa0054330 MtrunA17_Chr4g0004911 49.123 57 28 1 95 151 185 240 1.59e-13 69.3
Msa0054330 MtrunA17_Chr1g0150801 50.877 57 27 1 95 151 103 158 9.32e-17 75.1
Msa0054330 MtrunA17_Chr7g0249301 42.857 84 42 3 68 151 271 348 1.14e-16 78.2
Msa0054330 MtrunA17_Chr3g0139021 52.632 57 27 0 95 151 315 371 1.81e-16 77.8
Msa0054330 MtrunA17_Chr3g0079691 34.921 126 70 4 79 195 482 604 1.96e-16 78.2
Msa0054330 MtrunA17_Chr3g0079691 50.877 57 26 2 95 151 281 335 4.87e-12 65.1
Msa0054330 MtrunA17_Chr2g0284321 49.123 57 29 0 95 151 36 92 2.09e-16 75.5
Msa0054330 MtrunA17_Chr4g0071521 50.000 58 29 0 95 152 246 303 2.18e-16 77.0
Msa0054330 MtrunA17_Chr7g0224801 51.613 62 29 1 95 156 94 154 3.09e-16 73.9
Msa0054330 MtrunA17_Chr3g0129361 52.632 57 26 1 95 151 145 200 3.96e-16 74.7
Msa0054330 MtrunA17_Chr8g0383351 52.632 57 26 1 95 151 161 216 4.01e-16 75.1
Msa0054330 MtrunA17_Chr7g0243891 45.205 73 37 2 79 151 361 430 5.83e-16 76.6
Msa0054330 MtrunA17_Chr7g0243891 49.123 57 27 2 95 151 217 271 1.32e-11 63.9
Msa0054330 MtrunA17_Chr7g0249311 32.911 158 88 7 68 217 371 518 6.36e-16 76.6
Msa0054330 MtrunA17_Chr7g0249311 49.123 57 27 2 95 151 180 234 9.97e-11 61.2
Msa0054330 MtrunA17_Chr2g0289181 33.607 122 74 3 95 209 15 136 7.63e-16 74.3
Msa0054330 MtrunA17_Chr4g0068541 45.205 73 37 2 79 151 195 264 9.96e-16 75.9
Msa0054330 MtrunA17_Chr8g0335811 52.632 57 26 1 95 151 155 210 1.11e-15 73.6
Msa0054330 MtrunA17_Chr2g0319961 52.632 57 27 0 95 151 81 137 1.59e-15 73.6
Msa0054330 MtrunA17_Chr4g0074001 49.123 57 28 1 95 151 360 415 1.06e-14 72.8
Msa0054330 MtrunA17_Chr2g0293481 50.877 57 28 0 95 151 268 324 1.09e-14 72.8
Msa0054330 MtrunA17_Chr8g0386241 45.161 62 33 1 95 156 104 164 1.17e-14 69.7
Msa0054330 MtrunA17_Chr8g0387501 47.458 59 31 0 93 151 128 186 1.79e-14 70.9
Msa0054330 MtrunA17_Chr3g0129321 49.123 57 28 1 95 151 119 174 1.97e-14 69.7
Msa0054330 MtrunA17_Chr8g0383251 31.343 134 77 5 21 151 43 164 3.46e-14 68.6
Msa0054330 MtrunA17_Chr4g0024391 35.870 92 59 0 79 170 214 305 4.44e-14 70.9
Msa0054330 MtrunA17_Chr8g0348311 45.614 57 31 0 95 151 156 212 5.34e-14 70.1
Msa0054330 MtrunA17_Chr7g0248721 52.459 61 27 2 95 155 142 200 8.04e-14 68.2
Msa0054330 MtrunA17_Chr6g0485431 39.773 88 47 4 66 152 130 212 8.69e-14 69.7
Msa0054330 MtrunA17_Chr7g0270051 47.368 57 30 0 95 151 54 110 9.83e-14 68.9
Msa0054330 MtrunA17_Chr7g0248691 47.368 57 30 0 95 151 155 211 1.12e-13 69.3
Msa0054330 MtrunA17_Chr1g0202461 36.842 76 48 0 95 170 262 337 4.58e-13 67.8
Msa0054330 MtrunA17_Chr1g0189391 41.333 75 40 3 79 152 84 155 5.12e-13 67.4
Msa0054330 MtrunA17_Chr5g0447711 46.479 71 36 2 81 151 218 286 4.52e-12 64.7
Msa0054330 MtrunA17_Chr8g0387981 50.877 57 26 1 95 149 133 189 4.83e-12 64.7
Msa0054330 MtrunA17_Chr3g0126391 50.877 57 26 1 95 149 134 190 7.19e-12 63.9
Msa0054330 MtrunA17_Chr7g0275531 41.975 81 43 2 79 157 105 183 2.82e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0054330 AT1G80840.1 41.434 251 115 7 1 229 54 294 9.66e-56 180
Msa0054330 AT4G31800.2 54.706 170 67 4 66 233 82 243 1.59e-53 173
Msa0054330 AT4G31800.1 54.706 170 67 4 66 233 147 308 4.31e-53 174
Msa0054330 AT2G25000.2 51.786 168 63 3 68 233 75 226 1.26e-52 170
Msa0054330 AT2G25000.4 51.786 168 63 3 68 233 75 226 1.26e-52 170
Msa0054330 AT2G25000.3 51.786 168 63 3 68 233 97 248 6.98e-52 169
Msa0054330 AT2G25000.1 51.786 168 63 3 68 233 118 269 7.45e-52 169
Msa0054330 AT4G31800.3 71.642 67 18 1 66 131 147 213 6.08e-26 101
Msa0054330 AT4G04450.2 48.913 92 47 0 79 170 183 274 1.88e-24 100
Msa0054330 AT4G04450.1 48.913 92 47 0 79 170 277 368 4.65e-24 100
Msa0054330 AT1G68150.1 49.485 97 48 1 70 165 210 306 5.80e-24 99.0
Msa0054330 AT4G22070.4 54.795 73 33 0 79 151 282 354 4.34e-23 97.8
Msa0054330 AT4G22070.3 54.795 73 33 0 79 151 282 354 4.34e-23 97.8
Msa0054330 AT4G22070.2 54.795 73 33 0 79 151 282 354 4.34e-23 97.8
Msa0054330 AT4G22070.1 54.795 73 33 0 79 151 282 354 4.34e-23 97.8
Msa0054330 AT1G62300.1 54.795 73 33 0 79 151 297 369 7.85e-23 97.1
Msa0054330 AT4G01720.1 57.534 73 31 0 79 151 224 296 8.39e-23 96.7
Msa0054330 AT5G15130.2 52.703 74 35 0 95 168 186 259 3.06e-22 95.1
Msa0054330 AT5G15130.1 52.703 74 35 0 95 168 228 301 4.34e-22 94.7
Msa0054330 AT1G18860.1 64.912 57 20 0 95 151 240 296 1.46e-21 93.2
Msa0054330 AT4G18170.1 53.333 60 27 1 92 151 170 228 3.41e-19 85.1
Msa0054330 AT1G29860.1 53.333 60 27 1 92 151 134 192 3.97e-19 84.3
Msa0054330 AT1G64000.1 57.377 61 25 1 95 155 115 174 4.37e-19 82.4
Msa0054330 AT5G07100.2 38.614 101 58 3 51 151 101 197 9.55e-19 82.0
Msa0054330 AT5G07100.2 46.479 71 37 1 81 151 11 80 3.08e-14 69.7
Msa0054330 AT5G07100.4 38.614 101 58 3 51 151 101 197 9.55e-19 82.0
Msa0054330 AT5G07100.4 46.479 71 37 1 81 151 11 80 3.08e-14 69.7
Msa0054330 AT5G07100.5 38.614 101 58 3 51 151 101 197 9.55e-19 82.0
Msa0054330 AT5G07100.5 46.479 71 37 1 81 151 11 80 3.08e-14 69.7
Msa0054330 AT5G07100.3 38.614 101 58 3 51 151 101 197 9.55e-19 82.0
Msa0054330 AT5G07100.3 46.479 71 37 1 81 151 11 80 3.08e-14 69.7
Msa0054330 AT2G47260.1 55.000 60 26 1 92 151 172 230 9.91e-19 84.0
Msa0054330 AT5G26170.1 50.877 57 27 1 95 151 114 169 1.18e-18 80.9
Msa0054330 AT2G04880.2 57.627 59 24 1 93 151 282 339 1.19e-18 84.7
Msa0054330 AT2G04880.1 57.627 59 24 1 93 151 306 363 1.33e-18 84.7
Msa0054330 AT5G41570.1 57.377 61 25 1 95 155 99 158 1.72e-18 80.5
Msa0054330 AT4G26640.5 49.315 73 34 2 79 151 192 261 1.89e-18 83.6
Msa0054330 AT4G26640.6 49.315 73 34 2 79 151 192 261 1.89e-18 83.6
Msa0054330 AT4G26640.4 49.315 73 34 2 79 151 192 261 1.89e-18 83.6
Msa0054330 AT4G26640.3 49.315 73 34 2 79 151 192 261 1.89e-18 83.6
Msa0054330 AT1G13960.2 45.122 82 39 3 70 151 237 312 2.61e-18 83.2
Msa0054330 AT1G13960.2 52.632 57 25 2 95 151 77 131 4.40e-13 68.2
Msa0054330 AT4G26640.1 49.315 73 34 2 79 151 296 365 2.85e-18 83.6
Msa0054330 AT4G26640.7 49.315 73 34 2 79 151 328 397 3.10e-18 83.6
Msa0054330 AT4G26640.2 49.315 73 34 2 79 151 368 437 3.19e-18 83.6
Msa0054330 AT5G07100.1 38.614 101 58 3 51 151 194 290 4.14e-18 82.0
Msa0054330 AT5G07100.1 46.479 71 37 1 81 151 104 173 1.02e-13 69.7
Msa0054330 AT5G46350.1 47.222 72 33 2 80 151 173 239 4.38e-18 82.0
Msa0054330 AT2G03340.1 45.122 82 39 3 70 151 396 471 4.87e-18 83.2
Msa0054330 AT2G03340.1 52.632 57 25 2 95 151 251 305 8.98e-14 70.5
Msa0054330 AT1G13960.1 45.122 82 39 3 70 151 390 465 5.21e-18 82.8
Msa0054330 AT1G13960.1 52.632 57 25 2 95 151 230 284 5.16e-13 68.2
Msa0054330 AT4G31550.1 52.632 57 27 0 95 151 247 303 5.62e-18 81.6
Msa0054330 AT4G31550.2 52.632 57 27 0 95 151 246 302 5.62e-18 81.6
Msa0054330 AT5G49520.1 47.222 72 34 2 95 163 222 292 6.15e-18 82.4
Msa0054330 AT2G30590.1 54.386 57 26 0 95 151 314 370 6.46e-18 82.0
Msa0054330 AT1G69810.2 50.000 74 36 1 79 151 130 203 2.04e-17 80.1
Msa0054330 AT3G62340.1 51.562 64 30 1 88 151 112 174 2.10e-17 79.7
Msa0054330 AT1G69310.4 52.542 59 27 1 93 151 146 203 2.58e-17 79.3
Msa0054330 AT1G69310.2 52.542 59 27 1 93 151 146 203 2.58e-17 79.3
Msa0054330 AT1G69310.1 52.542 59 27 1 93 151 146 203 2.58e-17 79.3
Msa0054330 AT1G69810.1 50.000 74 36 1 79 151 188 261 2.74e-17 80.5
Msa0054330 AT5G56270.1 47.945 73 35 2 79 151 474 543 2.78e-17 80.9
Msa0054330 AT5G56270.1 30.137 146 94 4 6 151 191 328 9.17e-12 64.7
Msa0054330 AT2G24570.1 52.632 57 27 0 95 151 244 300 3.54e-17 79.3
Msa0054330 AT2G23320.1 49.123 57 29 0 95 151 241 297 3.54e-17 79.3
Msa0054330 AT5G13080.1 53.226 62 28 1 95 156 68 128 4.39e-17 75.9
Msa0054330 AT3G01080.1 43.902 82 40 3 70 151 287 362 4.67e-17 80.1
Msa0054330 AT3G01080.1 49.123 57 27 2 95 151 168 222 8.43e-11 61.6
Msa0054330 AT3G01080.2 43.902 82 40 3 70 151 325 400 5.14e-17 80.1
Msa0054330 AT3G01080.2 49.123 57 27 2 95 151 206 260 7.69e-11 62.0
Msa0054330 AT5G28650.1 43.023 86 41 2 82 165 248 327 5.54e-17 79.0
Msa0054330 AT1G29280.1 50.877 57 28 0 95 151 75 131 9.07e-17 77.4
Msa0054330 AT4G24240.1 48.276 58 30 0 95 152 282 339 1.01e-16 78.6
Msa0054330 AT3G04670.1 44.444 72 38 1 82 151 248 319 1.62e-16 77.8
Msa0054330 AT2G38470.1 45.205 73 37 2 79 151 349 418 1.86e-16 78.6
Msa0054330 AT2G38470.1 52.632 57 25 2 95 151 185 239 8.30e-14 70.5
Msa0054330 AT2G44745.1 52.632 57 26 1 95 151 146 201 7.63e-16 74.3
Msa0054330 AT3G01970.1 49.180 61 30 1 95 155 66 125 1.30e-15 72.0
Msa0054330 AT2G30250.1 45.205 73 37 2 79 151 315 384 1.33e-15 75.5
Msa0054330 AT2G30250.1 46.479 71 34 3 95 165 167 233 2.68e-11 63.2
Msa0054330 AT4G30935.1 50.877 57 27 1 95 151 332 387 1.34e-15 75.9
Msa0054330 AT2G37260.3 45.588 68 36 1 84 151 259 325 3.42e-15 74.3
Msa0054330 AT2G37260.3 45.070 71 37 2 81 151 72 140 1.01e-14 72.8
Msa0054330 AT2G37260.2 45.588 68 36 1 84 151 257 323 3.44e-15 74.3
Msa0054330 AT2G37260.2 45.070 71 37 2 81 151 70 138 9.71e-15 72.8
Msa0054330 AT2G37260.1 45.588 68 36 1 84 151 339 405 3.72e-15 74.3
Msa0054330 AT2G37260.1 45.070 71 37 2 81 151 152 220 1.09e-14 73.2
Msa0054330 AT1G55600.1 52.632 57 26 1 95 151 308 363 4.11e-15 74.3
Msa0054330 AT2G34830.2 47.692 65 34 0 87 151 217 281 5.96e-15 73.9
Msa0054330 AT2G34830.1 47.692 65 34 0 87 151 208 272 6.19e-15 73.9
Msa0054330 AT4G39410.1 50.877 57 27 1 95 151 224 279 7.73e-15 72.8
Msa0054330 AT2G21900.1 44.444 72 38 2 81 151 95 165 9.35e-15 70.9
Msa0054330 AT1G30650.1 47.692 65 34 0 87 151 210 274 1.21e-14 72.8
Msa0054330 AT3G58710.2 49.123 57 29 0 95 151 71 127 2.61e-14 70.9
Msa0054330 AT3G58710.1 49.123 57 29 0 95 151 72 128 2.62e-14 70.9
Msa0054330 AT5G64810.1 45.614 57 30 1 95 151 111 166 6.14e-14 68.6
Msa0054330 AT2G46130.1 50.877 57 27 1 95 151 31 86 7.56e-14 66.2
Msa0054330 AT4G01250.1 45.614 57 31 0 95 151 129 185 8.27e-14 69.7
Msa0054330 AT5G43290.1 40.789 76 43 2 76 151 97 170 1.31e-13 68.9
Msa0054330 AT4G26440.1 43.836 73 38 2 79 151 359 428 1.49e-13 70.1
Msa0054330 AT4G26440.1 47.368 57 28 2 95 151 179 233 5.90e-11 62.4
Msa0054330 AT5G52830.1 49.123 57 29 0 95 151 166 222 1.55e-13 69.3
Msa0054330 AT5G45050.1 34.951 103 64 1 49 151 1138 1237 2.88e-13 69.3
Msa0054330 AT5G45050.2 34.951 103 64 1 49 151 1110 1209 3.04e-13 69.3
Msa0054330 AT5G45050.4 34.951 103 64 1 49 151 999 1098 3.21e-13 69.3
Msa0054330 AT1G69310.3 49.153 59 25 2 93 151 146 199 7.53e-13 67.0
Msa0054330 AT4G23550.1 30.000 140 89 3 93 230 139 271 1.05e-12 66.6
Msa0054330 AT2G46400.1 40.698 86 46 3 66 149 79 161 4.22e-12 64.7
Msa0054330 AT5G24110.1 27.778 162 99 5 5 149 10 170 6.59e-11 61.6
Msa0054330 AT3G01080.3 49.123 57 27 2 95 151 206 260 7.53e-11 61.6

Find 46 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTATTTCTGGAAGAATTT+TGG 0.107436 1_2:-9092031 Msa0054330:CDS
GCACTTGCCACTGCTATTTC+TGG 0.167473 1_2:-9092042 Msa0054330:CDS
AATTGCTGGATAGATGATTT+TGG 0.173303 1_2:+9092117 None:intergenic
TGGAACATTATCAAGCTTAA+TGG 0.220468 1_2:+9092137 None:intergenic
TTCCTCACCTAAGGCTTATA+AGG 0.237117 1_2:-9093227 Msa0054330:CDS
AATGTCATTCTAAGAAATTT+AGG 0.271921 1_2:+9093554 None:intergenic
ATGTCATTCTAAGAAATTTA+GGG 0.314888 1_2:+9093555 None:intergenic
TCTTTCTTGATTGTTGTGTT+TGG 0.315541 1_2:+9093377 None:intergenic
GCAAGTGCTGTAGTGAAATT+AGG 0.319456 1_2:+9092057 None:intergenic
CAAAGTGAAGAAGCTCCTTT+AGG 0.328438 1_2:-9092210 Msa0054330:CDS
GGATATCAATGGAGGAAATA+TGG 0.328462 1_2:-9092993 Msa0054330:CDS
AATATGTTTGATACTGTTAG+AGG 0.330331 1_2:-9093328 Msa0054330:CDS
ATTTGTTCTTATTAGAAGTA+TGG 0.343403 1_2:+9092007 None:intergenic
TTATGTGCAGGACGAAGTTT+TGG 0.348061 1_2:-9093524 Msa0054330:five_prime_UTR
CCTCTCCTAGAGCTTACTTT+AGG 0.371319 1_2:-9092947 Msa0054330:CDS
TATGTTGCAACTAGTATAGT+AGG 0.404321 1_2:+9092288 None:intergenic
ATGAAGAATCATTAATCAAA+AGG 0.454669 1_2:-9093264 Msa0054330:CDS
CTAAAGGAGCTTCTTCACTT+TGG 0.478227 1_2:+9092211 None:intergenic
ATACTTCTAATAAGAACAAA+TGG 0.482703 1_2:-9092005 Msa0054330:CDS
ATGGAGGAAATATGGTCAAA+AGG 0.485930 1_2:-9092985 Msa0054330:CDS
ACAAGAACCTTATAAGCCTT+AGG 0.486641 1_2:+9093220 None:intergenic
ACATTATCAAGCTTAATGGT+TGG 0.490629 1_2:+9092141 None:intergenic
CATTAATCAAAAGGCCATGT+AGG 0.493040 1_2:-9093255 Msa0054330:CDS
ATACTAGTTGCAACATATGA+AGG 0.494693 1_2:-9092282 Msa0054330:CDS
TAGGGATATTTCCTCACCTA+AGG 0.500791 1_2:-9093236 Msa0054330:CDS
AACTGGGCAGCTTGGAGCAT+AGG 0.506437 1_2:+9092920 None:intergenic
GAGCACCTAAAGTAAGCTCT+AGG 0.538868 1_2:+9092942 None:intergenic
ATTAATCAAAAGGCCATGTA+GGG 0.538909 1_2:-9093254 Msa0054330:CDS
CCTAAAGTAAGCTCTAGGAG+AGG 0.548395 1_2:+9092947 None:intergenic
CTCATAGTTCTCACACATGT+TGG 0.554828 1_2:+9093447 None:intergenic
GTAACATGAACTGAACCTAA+AGG 0.560432 1_2:+9092195 None:intergenic
CTAAAGTAAGCTCTAGGAGA+GGG 0.569732 1_2:+9092948 None:intergenic
GTCTCAGTTCTGAGAACAAG+AGG 0.575865 1_2:-9093486 Msa0054330:five_prime_UTR
TTTCAGCTTTCTCATGACCA+TGG 0.582497 1_2:+9092250 None:intergenic
GGTATAAAGTATGTGATGGA+TGG 0.592987 1_2:-9093014 Msa0054330:intron
ATGTGATGGATGGATATCAA+TGG 0.593080 1_2:-9093004 Msa0054330:CDS
TTGTGGTATAAAGTATGTGA+TGG 0.595565 1_2:-9093018 Msa0054330:intron
ATGTTGCAACTAGTATAGTA+GGG 0.601976 1_2:+9092289 None:intergenic
TCAATGCACAACATTATACA+TGG 0.615653 1_2:+9091974 None:intergenic
GGTGAGGAAATATCCCTACA+TGG 0.620508 1_2:+9093241 None:intergenic
GTTCTTTGAATAATTTGTTG+AGG 0.631715 1_2:+9092168 None:intergenic
AACCTTATAAGCCTTAGGTG+AGG 0.658225 1_2:+9093225 None:intergenic
TAAAGTAAGCTCTAGGAGAG+GGG 0.664718 1_2:+9092949 None:intergenic
TGATGGATGGATATCAATGG+AGG 0.669535 1_2:-9093001 Msa0054330:CDS
AATTCTTCCAGAAATAGCAG+TGG 0.680346 1_2:+9092035 None:intergenic
TATGAAGGAGAGCACAACCA+TGG 0.694590 1_2:-9092267 Msa0054330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATTACAATAATTTAGT+AGG - chr1_2:9092636-9092655 Msa0054330:intron 10.0%
!!! TTGATTTGAAAAAAATAATT+CGG - chr1_2:9092793-9092812 Msa0054330:intron 10.0%
!! AAATATTACAATAATTTAGT+AGG - chr1_2:9092636-9092655 Msa0054330:intron 10.0%
!!! TTGATTTGAAAAAAATAATT+CGG - chr1_2:9092793-9092812 Msa0054330:intron 10.0%
!! TACAAATTCAAAATTAATCA+AGG + chr1_2:9092366-9092385 None:intergenic 15.0%
!!! TAATTTTGAATTTGTATTTG+TGG - chr1_2:9092370-9092389 Msa0054330:intron 15.0%
!!! ATCAAAATTTGATTTTGTTT+GGG + chr1_2:9093054-9093073 None:intergenic 15.0%
!! TACAAATTCAAAATTAATCA+AGG + chr1_2:9092366-9092385 None:intergenic 15.0%
!!! TAATTTTGAATTTGTATTTG+TGG - chr1_2:9092370-9092389 Msa0054330:intron 15.0%
!!! ATCAAAATTTGATTTTGTTT+GGG + chr1_2:9093054-9093073 None:intergenic 15.0%
!!! ATGTCATTCTAAGAAATTTA+GGG + chr1_2:9091853-9091872 None:intergenic 20.0%
!!! AATGTCATTCTAAGAAATTT+AGG + chr1_2:9091854-9091873 None:intergenic 20.0%
!! ATGAAGAATCATTAATCAAA+AGG - chr1_2:9092141-9092160 Msa0054330:CDS 20.0%
!!! TTATTACCTTCTTTTTAACT+GGG + chr1_2:9092504-9092523 None:intergenic 20.0%
!!! ATTATTACCTTCTTTTTAAC+TGG + chr1_2:9092505-9092524 None:intergenic 20.0%
!!! AGATGATTTTAAAACTTAGA+AGG + chr1_2:9092824-9092843 None:intergenic 20.0%
!!! GATCAAAATTTGATTTTGTT+TGG + chr1_2:9093055-9093074 None:intergenic 20.0%
!! CTCTTATGTCATTATTAATT+AGG - chr1_2:9093074-9093093 Msa0054330:intron 20.0%
!! ATTTGTTCTTATTAGAAGTA+TGG + chr1_2:9093401-9093420 None:intergenic 20.0%
!! ATACTTCTAATAAGAACAAA+TGG - chr1_2:9093400-9093419 Msa0054330:CDS 20.0%
!!! ATGTCATTCTAAGAAATTTA+GGG + chr1_2:9091853-9091872 None:intergenic 20.0%
!!! AATGTCATTCTAAGAAATTT+AGG + chr1_2:9091854-9091873 None:intergenic 20.0%
!! ATGAAGAATCATTAATCAAA+AGG - chr1_2:9092141-9092160 Msa0054330:CDS 20.0%
!!! TTATTACCTTCTTTTTAACT+GGG + chr1_2:9092504-9092523 None:intergenic 20.0%
!!! ATTATTACCTTCTTTTTAAC+TGG + chr1_2:9092505-9092524 None:intergenic 20.0%
!!! AGATGATTTTAAAACTTAGA+AGG + chr1_2:9092824-9092843 None:intergenic 20.0%
!!! GATCAAAATTTGATTTTGTT+TGG + chr1_2:9093055-9093074 None:intergenic 20.0%
!! CTCTTATGTCATTATTAATT+AGG - chr1_2:9093074-9093093 Msa0054330:intron 20.0%
!! ATTTGTTCTTATTAGAAGTA+TGG + chr1_2:9093401-9093420 None:intergenic 20.0%
!! ATACTTCTAATAAGAACAAA+TGG - chr1_2:9093400-9093419 Msa0054330:CDS 20.0%
!! ACATTGAAAATTTTATGTGC+AGG - chr1_2:9091869-9091888 Msa0054330:three_prime_UTR 25.0%
! AATATGTTTGATACTGTTAG+AGG - chr1_2:9092077-9092096 Msa0054330:CDS 25.0%
! CAGTTAATGAAGTAAAATTG+TGG - chr1_2:9092321-9092340 Msa0054330:intron 25.0%
! TTTCGATAGAGATTACTAAT+CGG + chr1_2:9092617-9092636 None:intergenic 25.0%
! AGTCTTTATATGAAAAGATG+TGG + chr1_2:9092675-9092694 None:intergenic 25.0%
! TACTTAGACCAATATATTGT+TGG - chr1_2:9092734-9092753 Msa0054330:intron 25.0%
! CATGTTTACCAACAATATAT+TGG + chr1_2:9092745-9092764 None:intergenic 25.0%
! AGAAACAGAAGTAAAAAGAT+AGG - chr1_2:9092924-9092943 Msa0054330:CDS 25.0%
! TCTTGAATCAAAATCATGAA+TGG + chr1_2:9093027-9093046 None:intergenic 25.0%
!!! GTTCTTTGAATAATTTGTTG+AGG + chr1_2:9093240-9093259 None:intergenic 25.0%
!! ACATTGAAAATTTTATGTGC+AGG - chr1_2:9091869-9091888 Msa0054330:three_prime_UTR 25.0%
! AATATGTTTGATACTGTTAG+AGG - chr1_2:9092077-9092096 Msa0054330:CDS 25.0%
! CAGTTAATGAAGTAAAATTG+TGG - chr1_2:9092321-9092340 Msa0054330:intron 25.0%
! TTTCGATAGAGATTACTAAT+CGG + chr1_2:9092617-9092636 None:intergenic 25.0%
! AGTCTTTATATGAAAAGATG+TGG + chr1_2:9092675-9092694 None:intergenic 25.0%
! TACTTAGACCAATATATTGT+TGG - chr1_2:9092734-9092753 Msa0054330:intron 25.0%
! CATGTTTACCAACAATATAT+TGG + chr1_2:9092745-9092764 None:intergenic 25.0%
! AGAAACAGAAGTAAAAAGAT+AGG - chr1_2:9092924-9092943 Msa0054330:CDS 25.0%
! TCTTGAATCAAAATCATGAA+TGG + chr1_2:9093027-9093046 None:intergenic 25.0%
!!! GTTCTTTGAATAATTTGTTG+AGG + chr1_2:9093240-9093259 None:intergenic 25.0%
TCTTTCTTGATTGTTGTGTT+TGG + chr1_2:9092031-9092050 None:intergenic 30.0%
ATTAATCAAAAGGCCATGTA+GGG - chr1_2:9092151-9092170 Msa0054330:CDS 30.0%
TTGTGGTATAAAGTATGTGA+TGG - chr1_2:9092387-9092406 Msa0054330:intron 30.0%
TATGAAAAGATGTGGTTGAT+AGG + chr1_2:9092667-9092686 None:intergenic 30.0%
ATGTTGCAACTAGTATAGTA+GGG + chr1_2:9093119-9093138 None:intergenic 30.0%
TATGTTGCAACTAGTATAGT+AGG + chr1_2:9093120-9093139 None:intergenic 30.0%
ATACTAGTTGCAACATATGA+AGG - chr1_2:9093123-9093142 Msa0054330:intron 30.0%
ACATTATCAAGCTTAATGGT+TGG + chr1_2:9093267-9093286 None:intergenic 30.0%
TGGAACATTATCAAGCTTAA+TGG + chr1_2:9093271-9093290 None:intergenic 30.0%
! AATTGCTGGATAGATGATTT+TGG + chr1_2:9093291-9093310 None:intergenic 30.0%
! ATCATCTATCCAGCAATTTT+TGG - chr1_2:9093293-9093312 Msa0054330:CDS 30.0%
TTTGTTGAACCAAAAATTGC+TGG + chr1_2:9093305-9093324 None:intergenic 30.0%
!!! GCAATTTTTGGTTCAACAAA+TGG - chr1_2:9093305-9093324 Msa0054330:CDS 30.0%
!!! TGCTATTTCTGGAAGAATTT+TGG - chr1_2:9093374-9093393 Msa0054330:CDS 30.0%
TCAATGCACAACATTATACA+TGG + chr1_2:9093434-9093453 None:intergenic 30.0%
TCTTTCTTGATTGTTGTGTT+TGG + chr1_2:9092031-9092050 None:intergenic 30.0%
ATTAATCAAAAGGCCATGTA+GGG - chr1_2:9092151-9092170 Msa0054330:CDS 30.0%
TTGTGGTATAAAGTATGTGA+TGG - chr1_2:9092387-9092406 Msa0054330:intron 30.0%
TATGAAAAGATGTGGTTGAT+AGG + chr1_2:9092667-9092686 None:intergenic 30.0%
ATGTTGCAACTAGTATAGTA+GGG + chr1_2:9093119-9093138 None:intergenic 30.0%
TATGTTGCAACTAGTATAGT+AGG + chr1_2:9093120-9093139 None:intergenic 30.0%
ATACTAGTTGCAACATATGA+AGG - chr1_2:9093123-9093142 Msa0054330:intron 30.0%
ACATTATCAAGCTTAATGGT+TGG + chr1_2:9093267-9093286 None:intergenic 30.0%
TGGAACATTATCAAGCTTAA+TGG + chr1_2:9093271-9093290 None:intergenic 30.0%
! AATTGCTGGATAGATGATTT+TGG + chr1_2:9093291-9093310 None:intergenic 30.0%
! ATCATCTATCCAGCAATTTT+TGG - chr1_2:9093293-9093312 Msa0054330:CDS 30.0%
TTTGTTGAACCAAAAATTGC+TGG + chr1_2:9093305-9093324 None:intergenic 30.0%
!!! GCAATTTTTGGTTCAACAAA+TGG - chr1_2:9093305-9093324 Msa0054330:CDS 30.0%
!!! TGCTATTTCTGGAAGAATTT+TGG - chr1_2:9093374-9093393 Msa0054330:CDS 30.0%
TCAATGCACAACATTATACA+TGG + chr1_2:9093434-9093453 None:intergenic 30.0%
CATTAATCAAAAGGCCATGT+AGG - chr1_2:9092150-9092169 Msa0054330:CDS 35.0%
ACAAGAACCTTATAAGCCTT+AGG + chr1_2:9092188-9092207 None:intergenic 35.0%
! GGTATAAAGTATGTGATGGA+TGG - chr1_2:9092391-9092410 Msa0054330:intron 35.0%
!! ATGTGATGGATGGATATCAA+TGG - chr1_2:9092401-9092420 Msa0054330:intron 35.0%
GGATATCAATGGAGGAAATA+TGG - chr1_2:9092412-9092431 Msa0054330:intron 35.0%
ATGGAGGAAATATGGTCAAA+AGG - chr1_2:9092420-9092439 Msa0054330:intron 35.0%
AGATTACTAATCGGTGAAGT+AGG + chr1_2:9092608-9092627 None:intergenic 35.0%
GTAACATGAACTGAACCTAA+AGG + chr1_2:9093213-9093232 None:intergenic 35.0%
AATTCTTCCAGAAATAGCAG+TGG + chr1_2:9093373-9093392 None:intergenic 35.0%
CATTAATCAAAAGGCCATGT+AGG - chr1_2:9092150-9092169 Msa0054330:CDS 35.0%
ACAAGAACCTTATAAGCCTT+AGG + chr1_2:9092188-9092207 None:intergenic 35.0%
! GGTATAAAGTATGTGATGGA+TGG - chr1_2:9092391-9092410 Msa0054330:intron 35.0%
!! ATGTGATGGATGGATATCAA+TGG - chr1_2:9092401-9092420 Msa0054330:intron 35.0%
GGATATCAATGGAGGAAATA+TGG - chr1_2:9092412-9092431 Msa0054330:intron 35.0%
ATGGAGGAAATATGGTCAAA+AGG - chr1_2:9092420-9092439 Msa0054330:intron 35.0%
AGATTACTAATCGGTGAAGT+AGG + chr1_2:9092608-9092627 None:intergenic 35.0%
GTAACATGAACTGAACCTAA+AGG + chr1_2:9093213-9093232 None:intergenic 35.0%
AATTCTTCCAGAAATAGCAG+TGG + chr1_2:9093373-9093392 None:intergenic 35.0%
! TTATGTGCAGGACGAAGTTT+TGG - chr1_2:9091881-9091900 Msa0054330:three_prime_UTR 40.0%
CTCATAGTTCTCACACATGT+TGG + chr1_2:9091961-9091980 None:intergenic 40.0%
TAGGGATATTTCCTCACCTA+AGG - chr1_2:9092169-9092188 Msa0054330:CDS 40.0%
TTCCTCACCTAAGGCTTATA+AGG - chr1_2:9092178-9092197 Msa0054330:CDS 40.0%
AACCTTATAAGCCTTAGGTG+AGG + chr1_2:9092183-9092202 None:intergenic 40.0%
! TGATGGATGGATATCAATGG+AGG - chr1_2:9092404-9092423 Msa0054330:intron 40.0%
!! TAAAGTAAGCTCTAGGAGAG+GGG + chr1_2:9092459-9092478 None:intergenic 40.0%
!! CTAAAGTAAGCTCTAGGAGA+GGG + chr1_2:9092460-9092479 None:intergenic 40.0%
!! TTCTTTTTAACTGGGCAGCT+TGG + chr1_2:9092496-9092515 None:intergenic 40.0%
AAGCTGCCCAGTTAAAAAGA+AGG - chr1_2:9092495-9092514 Msa0054330:intron 40.0%
TTACTAATCGGTGAAGTAGG+TGG + chr1_2:9092605-9092624 None:intergenic 40.0%
TTTCAGCTTTCTCATGACCA+TGG + chr1_2:9093158-9093177 None:intergenic 40.0%
CTAAAGGAGCTTCTTCACTT+TGG + chr1_2:9093197-9093216 None:intergenic 40.0%
CAAAGTGAAGAAGCTCCTTT+AGG - chr1_2:9093195-9093214 Msa0054330:CDS 40.0%
! GCAAGTGCTGTAGTGAAATT+AGG + chr1_2:9093351-9093370 None:intergenic 40.0%
! TTATGTGCAGGACGAAGTTT+TGG - chr1_2:9091881-9091900 Msa0054330:three_prime_UTR 40.0%
CTCATAGTTCTCACACATGT+TGG + chr1_2:9091961-9091980 None:intergenic 40.0%
TAGGGATATTTCCTCACCTA+AGG - chr1_2:9092169-9092188 Msa0054330:CDS 40.0%
TTCCTCACCTAAGGCTTATA+AGG - chr1_2:9092178-9092197 Msa0054330:CDS 40.0%
AACCTTATAAGCCTTAGGTG+AGG + chr1_2:9092183-9092202 None:intergenic 40.0%
! TGATGGATGGATATCAATGG+AGG - chr1_2:9092404-9092423 Msa0054330:intron 40.0%
!! TAAAGTAAGCTCTAGGAGAG+GGG + chr1_2:9092459-9092478 None:intergenic 40.0%
!! CTAAAGTAAGCTCTAGGAGA+GGG + chr1_2:9092460-9092479 None:intergenic 40.0%
!! TTCTTTTTAACTGGGCAGCT+TGG + chr1_2:9092496-9092515 None:intergenic 40.0%
AAGCTGCCCAGTTAAAAAGA+AGG - chr1_2:9092495-9092514 Msa0054330:intron 40.0%
TTACTAATCGGTGAAGTAGG+TGG + chr1_2:9092605-9092624 None:intergenic 40.0%
TTTCAGCTTTCTCATGACCA+TGG + chr1_2:9093158-9093177 None:intergenic 40.0%
CTAAAGGAGCTTCTTCACTT+TGG + chr1_2:9093197-9093216 None:intergenic 40.0%
CAAAGTGAAGAAGCTCCTTT+AGG - chr1_2:9093195-9093214 Msa0054330:CDS 40.0%
! GCAAGTGCTGTAGTGAAATT+AGG + chr1_2:9093351-9093370 None:intergenic 40.0%
!! GTCTCAGTTCTGAGAACAAG+AGG - chr1_2:9091919-9091938 Msa0054330:three_prime_UTR 45.0%
GGTGAGGAAATATCCCTACA+TGG + chr1_2:9092167-9092186 None:intergenic 45.0%
!! CCTAAAGTAAGCTCTAGGAG+AGG + chr1_2:9092461-9092480 None:intergenic 45.0%
CCTCTCCTAGAGCTTACTTT+AGG - chr1_2:9092458-9092477 Msa0054330:intron 45.0%
GAGCACCTAAAGTAAGCTCT+AGG + chr1_2:9092466-9092485 None:intergenic 45.0%
! TATGAAGGAGAGCACAACCA+TGG - chr1_2:9093138-9093157 Msa0054330:intron 45.0%
!! GTCTCAGTTCTGAGAACAAG+AGG - chr1_2:9091919-9091938 Msa0054330:three_prime_UTR 45.0%
GGTGAGGAAATATCCCTACA+TGG + chr1_2:9092167-9092186 None:intergenic 45.0%
!! CCTAAAGTAAGCTCTAGGAG+AGG + chr1_2:9092461-9092480 None:intergenic 45.0%
CCTCTCCTAGAGCTTACTTT+AGG - chr1_2:9092458-9092477 Msa0054330:intron 45.0%
GAGCACCTAAAGTAAGCTCT+AGG + chr1_2:9092466-9092485 None:intergenic 45.0%
! TATGAAGGAGAGCACAACCA+TGG - chr1_2:9093138-9093157 Msa0054330:intron 45.0%
!! GCACTTGCCACTGCTATTTC+TGG - chr1_2:9093363-9093382 Msa0054330:CDS 50.0%
!! GCACTTGCCACTGCTATTTC+TGG - chr1_2:9093363-9093382 Msa0054330:CDS 50.0%
AACTGGGCAGCTTGGAGCAT+AGG + chr1_2:9092488-9092507 None:intergenic 55.0%
AACTGGGCAGCTTGGAGCAT+AGG + chr1_2:9092488-9092507 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 9091849 9093578 9091849 ID=Msa0054330;Name=Msa0054330
chr1_2 mRNA 9091849 9093578 9091849 ID=Msa0054330-mRNA-1;Parent=Msa0054330;Name=Msa0054330-mRNA-1;_AED=0.32;_eAED=0.32;_QI=114|1|1|1|1|1|3|153|239
chr1_2 exon 9091849 9092331 9091849 ID=Msa0054330-mRNA-1:exon:3324;Parent=Msa0054330-mRNA-1
chr1_2 exon 9092911 9093027 9092911 ID=Msa0054330-mRNA-1:exon:3323;Parent=Msa0054330-mRNA-1
chr1_2 exon 9093192 9093578 9093192 ID=Msa0054330-mRNA-1:exon:3322;Parent=Msa0054330-mRNA-1
chr1_2 five_prime_UTR 9093465 9093578 9093465 ID=Msa0054330-mRNA-1:five_prime_utr;Parent=Msa0054330-mRNA-1
chr1_2 CDS 9093192 9093464 9093192 ID=Msa0054330-mRNA-1:cds;Parent=Msa0054330-mRNA-1
chr1_2 CDS 9092911 9093027 9092911 ID=Msa0054330-mRNA-1:cds;Parent=Msa0054330-mRNA-1
chr1_2 CDS 9092002 9092331 9092002 ID=Msa0054330-mRNA-1:cds;Parent=Msa0054330-mRNA-1
chr1_2 three_prime_UTR 9091849 9092001 9091849 ID=Msa0054330-mRNA-1:three_prime_utr;Parent=Msa0054330-mRNA-1
Gene Sequence

>Msa0054330

ATGTGTGAGAACTATGAGACTATGCAAAAGCAATTGAATCAATTGATGAATCAAAATTTTGAACACCAAACACAACAATCAAGAAAGAGAAAAGCTGAGAGTGAAAGTTGCATCAATATGTTTGATACTGTTAGAGGCATTAATATTATCAATAATAATGAGTGCAGCACTGTTACTGATGAAGAATCATTAATCAAAAGGCCATGTAGGGATATTTCCTCACCTAAGGCTTATAAGGTTCTTGTGAAGACAGAAGCATCTAGTAATAGCTTATATGTGATGGATGGATATCAATGGAGGAAATATGGTCAAAAGGTTACTAGAGATAACCCCTCTCCTAGAGCTTACTTTAGGTGCTCCTATGCTCCAAGCTGCCCAGTTAAAAAGAAGGTGCAAAAAAGTTTAGAAGACCCTACTATACTAGTTGCAACATATGAAGGAGAGCACAACCATGGTCATGAGAAAGCTGAAATATCAATGATATCAAGCCAAAGTGAAGAAGCTCCTTTAGGTTCAGTTCATGTTACTTCACCTCAACAAATTATTCAAAGAACATGTCCAACCATTAAGCTTGATAATGTTCCAAAATCATCTATCCAGCAATTTTTGGTTCAACAAATGGCTACTTCTTTGACAAATGATCCTAATTTCACTACAGCACTTGCCACTGCTATTTCTGGAAGAATTTTGGACCATACTTCTAATAAGAACAAATGGTGA
Protein sequence

>Msa0054330

MCENYETMQKQLNQLMNQNFEHQTQQSRKRKAESESCINMFDTVRGINIINNNECSTVTDEESLIKRPCRDISSPKAYKVLVKTEASSNSLYVMDGYQWRKYGQKVTRDNPSPRAYFRCSYAPSCPVKKKVQKSLEDPTILVATYEGEHNHGHEKAEISMISSQSEEAPLGSVHVTSPQQIIQRTCPTIKLDNVPKSSIQQFLVQQMATSLTNDPNFTTALATAISGRILDHTSNKNKW*