AlfalfaGEDB | Msa0072530

M. sativa cultivar zhongmu-4 / Msa0072530

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0072530	XP_003590392.1	99.023	512	2	1	1	509	1	512	0.0	1056

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0072530	sp\|Q9LSA9\|PUMPK_ARATH	53.906	256	113	2	252	505	78	330	9.80e-90	281

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0072530	A7UQU8	99.023	512	2	1	1	509	1	512	0.0	1056

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TFs/TRs:

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Gene ID	Type	Classification
Msa0072530	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0072530	Msa0120800	0.906546	1.322297e-80	-8.615850e-47
Msa0072530	Msa0910620	0.801896	7.252794e-49	-8.615850e-47
Msa0072530	Msa1042660	0.801138	1.037132e-48	-8.615850e-47
Msa0072530	Msa1400930	0.806667	7.359428e-50	-8.615850e-47
Msa0072530	Msa1428780	0.800640	1.310737e-48	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0072530	MtrunA17_Chr1g0179531	99.023	512	2	1	1	509	1	512	0.0	1056
Msa0072530	MtrunA17_Chr8g0384081	50.758	264	120	3	249	505	56	316	1.62e-87	272
Msa0072530	MtrunA17_Chr2g0283871	35.644	101	59	3	130	225	23	122	1.13e-15	78.2
Msa0072530	MtrunA17_Chr4g0041151	36.275	102	58	3	130	225	31	131	1.28e-15	77.8
Msa0072530	MtrunA17_Chr1g0164461	41.346	104	51	4	118	218	27	123	1.58e-15	75.1
Msa0072530	MtrunA17_Chr6g0485241	37.255	102	61	2	128	227	128	228	2.43e-15	77.8
Msa0072530	MtrunA17_Chr3g0082261	41.346	104	51	4	118	218	27	123	1.76e-14	74.7
Msa0072530	MtrunA17_Chr5g0402321	34.000	100	60	2	130	223	22	121	1.40e-13	70.5
Msa0072530	MtrunA17_Chr4g0066271	38.000	100	58	2	130	225	117	216	3.48e-12	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0072530	AT3G10030.1	56.364	550	184	7	1	506	1	538	0.0	590
Msa0072530	AT3G10030.2	55.086	521	178	7	1	477	1	509	0.0	542
Msa0072530	AT3G18680.1	53.906	256	113	2	252	505	78	330	9.97e-91	281
Msa0072530	AT3G18680.2	47.751	289	113	3	252	505	78	363	1.64e-83	263
Msa0072530	AT3G54390.1	36.441	118	65	3	124	239	32	141	3.35e-17	82.8
Msa0072530	AT3G54390.2	36.441	118	65	3	124	239	48	157	3.76e-17	82.8
Msa0072530	AT3G58630.1	30.872	149	78	3	130	253	26	174	2.71e-16	80.5
Msa0072530	AT3G58630.2	30.872	149	78	3	130	253	26	174	3.07e-16	80.1
Msa0072530	AT3G14180.1	37.607	117	66	4	109	221	63	176	5.91e-16	80.5
Msa0072530	AT3G24490.1	31.667	120	73	3	102	218	64	177	1.42e-12	69.3

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Find 174 sgRNAs with CRISPR-Local

Find 338 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCGGTTTCAAGCGGTAA+CGG	0.521565	1_2:-45557015	Msa0072530:CDS
AAAGGAAGAGTTGTTATATT+TGG	0.225653	1_2:-45554243	Msa0072530:CDS
AAATTTACGCAGCTGAATCG+AGG	0.599706	1_2:-45556898	Msa0072530:CDS
AACATTTCAAAAGCTTTGTG+TGG	0.537883	1_2:-45553476	Msa0072530:CDS
AACCTTAGGGTCAATATTGC+AGG	0.385341	1_2:+45555669	None:intergenic
AACTTCATTTGAAACTAACA+TGG	0.586306	1_2:+45555497	None:intergenic
AAGACGGTGAAAGGGTTTGA+AGG	0.545461	1_2:+45557369	None:intergenic
AAGCAGATGGAACATATTGT+TGG	0.431908	1_2:-45556697	Msa0072530:CDS
AAGCATACGAAAACGGAATT+TGG	0.135590	1_2:+45553312	None:intergenic
AAGCATTGTCAAACCTTAGA+TGG	0.420124	1_2:-45555741	Msa0072530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGAGACATAGAATGAATAA+TGG	-	chr1_2:45553901-45553920	Msa0072530:CDS	30.0%
TTTATCATCATCAGCAAACT+TGG	+	chr1_2:45553995-45554014	None:intergenic	30.0%
AAAGTAAACATGTAAGTCCT+GGG	+	chr1_2:45554417-45554436	None:intergenic	30.0%
TAAAGTAAACATGTAAGTCC+TGG	+	chr1_2:45554418-45554437	None:intergenic	30.0%
TTGATTGTGTTGTCATTGTT+TGG	-	chr1_2:45554738-45554757	Msa0072530:intron	30.0%
AGTTTCAAATGAAGTTGCAA+TGG	-	chr1_2:45555162-45555181	Msa0072530:intron	30.0%
ATTTGTAATCAGGATGTTCA+TGG	+	chr1_2:45555498-45555517	None:intergenic	30.0%
ATGAACATCCTGATTACAAA+TGG	-	chr1_2:45555497-45555516	Msa0072530:CDS	30.0%
TGAACATCCTGATTACAAAT+GGG	-	chr1_2:45555498-45555517	Msa0072530:CDS	30.0%
TTCAATATGCTGCTAAACAA+AGG	+	chr1_2:45555895-45555914	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	45553122	45557552	45553122	ID=Msa0072530;Name=Msa0072530
chr1_2	mRNA	45553122	45557552	45553122	ID=Msa0072530-mRNA-1;Parent=Msa0072530;Name=Msa0072530-mRNA-1;_AED=0.07;_eAED=0.07;_QI=200\|1\|1\|1\|1\|1\|7\|308\|509
chr1_2	exon	45553122	45553527	45553122	ID=Msa0072530-mRNA-1:exon:13751;Parent=Msa0072530-mRNA-1
chr1_2	exon	45553872	45554042	45553872	ID=Msa0072530-mRNA-1:exon:13750;Parent=Msa0072530-mRNA-1
chr1_2	exon	45554172	45554407	45554172	ID=Msa0072530-mRNA-1:exon:13749;Parent=Msa0072530-mRNA-1
chr1_2	exon	45555288	45555379	45555288	ID=Msa0072530-mRNA-1:exon:13748;Parent=Msa0072530-mRNA-1
chr1_2	exon	45555470	45555523	45555470	ID=Msa0072530-mRNA-1:exon:13747;Parent=Msa0072530-mRNA-1
chr1_2	exon	45555672	45555816	45555672	ID=Msa0072530-mRNA-1:exon:13746;Parent=Msa0072530-mRNA-1
chr1_2	exon	45556619	45557552	45556619	ID=Msa0072530-mRNA-1:exon:13745;Parent=Msa0072530-mRNA-1
chr1_2	five_prime_UTR	45557353	45557552	45557353	ID=Msa0072530-mRNA-1:five_prime_utr;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45556619	45557352	45556619	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45555672	45555816	45555672	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45555470	45555523	45555470	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45555288	45555379	45555288	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45554172	45554407	45554172	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45553872	45554042	45553872	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	CDS	45553430	45553527	45553430	ID=Msa0072530-mRNA-1:cds;Parent=Msa0072530-mRNA-1
chr1_2	three_prime_UTR	45553122	45553429	45553122	ID=Msa0072530-mRNA-1:three_prime_utr;Parent=Msa0072530-mRNA-1

Gene Sequence

>Msa0072530

ATGGCTTCTTGCGACGACGACGACGATTTCTCTCTCCTCCACGACGATAACAACCACCACTCTCACCACCACCACCTCCACCAAACCTTCGCACCTCACCACCACCACTCTCACCACCACTTCTCCACAGCTTCAGTCCAATCCAAACACATCACCACCTCCGTCTCCGCCGCCGTCGCCGACGACGACGGAGACGACACCGATGACTACGGTAATCCATTCGACGATGACGCTGGTAGCGAGAAACGTAAAGATCGAGATCGCGATGAGATCTGTGATGCAACGACGTCGTATGGATTCAACAACAAACGGTCAAAACCGGTTTCAAGCGGTAACGGTGGAGGAAGCGCCGGAGTAGGAGCAGAGTATAGAAAGGATCGAGAAGAATGGAGTGATACAGCGATAGTGTGTTTACTCGAAGCTTATACAGAGAAATTTACGCAGCTGAATCGAGGGAATCTAAGAGGAAGAGACTGGGAAGAAGTAGCTTCAATGGTGAGTGAACGGTGTGAGAATCAATCAAAGAGTGTGGAACAGTGTAAGAACAAGGTTGATAATTTGAAGAAAAGGTATAAGCTAGAGAGACATAGAATGAATAATGGTTGCATTTCTGCTAGTCATTGGCCTTGGTTTAAGCAGATGGAACATATTGTTGGGAATTCGCTTCCGGCCAAGTTTGCTGATGATGATAAAGCTATTGTTCCTGCTTCAATTTCACCTAGGCAATCTAAGAGATATGGAGTGGCTACGTCCAGCAGTGGAGGTCAAGTAAACAGCCTGAAGTCCAAAGCATTGTCAAACCTTAGATGGCGGAGAGTTGTTCTAAAAATTAGTGGATCGGCACTTACCGGATCCGATACCTGCAATATTGACCCTAAGGTCGCCATGTTAGTTTCAAATGAAGTTGCAATGGCTTCCCGTCTTGGTGTGGAGGTAGCAATTGTTGTTGGAGGTCGTAATTTCTTCTGTGGTGATGCATGGGTAACTGCAACAGGTTTAGAAAGATGTACTGCATACCAAGTTGGCATGATGGCAACTGTGATGAACTCCTTACTGCTTCAATCAACTTTAGAGAAGATGGGTGTTCAGACTCGTGTACAAACTTCAGTTTCAATGCAGGCGTTTGCTGAACCGTACAATAGGCAACGGGCCATCAGGCATCTTGAGAAAGGAAGAGTTGTTATATTTGGTGGCATTGGTTTTGGTGCTGGTAATCCACTTTTCTCAACCGACATATCTGCTGCTCTTCGGGCTTCAGAGGTTAATGCTCAGGCTGTCCTCAAAGGTACCAACGTAGATGGCGTATATGACTGCAACTCACGAGACAACAATTTCACTTTCGAGCACATATCCTTCAGGGAGCTAGTGTCTAGAGGTGTCACCTCTATGGATATGTCAGCCCTGACATTCTGCGAGGAAAATGCCATTCCTGTTGTAGTTTTTAATCTATTGGCACCCGGGAACATTTCAAAAGCTTTGTGTGGAGAACAAGTTGGCACACTTATTGACCAAACTGGAGCAATAAGCTAA

Protein sequence

>Msa0072530

MASCDDDDDFSLLHDDNNHHSHHHHLHQTFAPHHHHSHHHFSTASVQSKHITTSVSAAVADDDGDDTDDYGNPFDDDAGSEKRKDRDRDEICDATTSYGFNNKRSKPVSSGNGGGSAGVGAEYRKDREEWSDTAIVCLLEAYTEKFTQLNRGNLRGRDWEEVASMVSERCENQSKSVEQCKNKVDNLKKRYKLERHRMNNGCISASHWPWFKQMEHIVGNSLPAKFADDDKAIVPASISPRQSKRYGVATSSSGGQVNSLKSKALSNLRWRRVVLKISGSALTGSDTCNIDPKVAMLVSNEVAMASRLGVEVAIVVGGRNFFCGDAWVTATGLERCTAYQVGMMATVMNSLLLQSTLEKMGVQTRVQTSVSMQAFAEPYNRQRAIRHLEKGRVVIFGGIGFGAGNPLFSTDISAALRASEVNAQAVLKGTNVDGVYDCNSRDNNFTFEHISFRELVSRGVTSMDMSALTFCEENAIPVVVFNLLAPGNISKALCGEQVGTLIDQTGAIS*