AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0077300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077300 MtrunA17_Chr1g0186561 96.359 357 13 0 1 357 1 357 0.0 702
Msa0077300 MtrunA17_Chr1g0186601 87.135 342 43 1 1 342 1 341 0.0 614
Msa0077300 MtrunA17_Chr1g0186571 79.272 357 70 2 1 357 1 353 0.0 583
Msa0077300 MtrunA17_Chr1g0186611 79.772 351 70 1 1 351 1 350 0.0 580
Msa0077300 MtrunA17_Chr1g0186641 80.925 346 62 2 1 346 1 342 0.0 575
Msa0077300 MtrunA17_Chr1g0171301 74.581 358 87 2 1 357 1 355 0.0 545
Msa0077300 MtrunA17_Chr1g0184221 52.312 346 145 4 11 342 11 350 2.33e-127 370
Msa0077300 MtrunA17_Chr7g0255561 44.578 332 174 4 12 336 10 338 4.25e-94 286
Msa0077300 MtrunA17_Chr7g0255581 44.578 332 174 4 12 336 10 338 4.25e-94 286
Msa0077300 MtrunA17_Chr7g0263101 44.848 330 173 5 12 336 9 334 7.96e-93 282
Msa0077300 MtrunA17_Chr1g0182291 42.547 322 184 1 13 334 12 332 1.42e-90 276
Msa0077300 MtrunA17_Chr1g0160171 43.807 331 168 3 13 334 13 334 1.69e-89 273
Msa0077300 MtrunA17_Chr3g0114691 38.372 344 200 3 13 349 17 355 3.73e-85 263
Msa0077300 MtrunA17_Chr1g0160181 40.854 328 191 1 12 336 14 341 1.52e-84 261
Msa0077300 MtrunA17_Chr3g0114731 40.356 337 196 4 16 351 18 350 2.50e-83 258
Msa0077300 MtrunA17_Chr3g0081421 42.202 327 178 5 16 334 17 340 4.93e-82 255
Msa0077300 MtrunA17_Chr3g0081511 39.886 351 201 4 12 353 13 362 5.84e-81 252
Msa0077300 MtrunA17_Chr3g0142131 39.941 338 188 3 13 336 17 353 3.31e-80 250
Msa0077300 MtrunA17_Chr8g0354121 38.997 359 196 8 12 354 9 360 2.13e-79 248
Msa0077300 MtrunA17_Chr3g0114711 34.530 362 201 3 16 341 14 375 4.05e-76 239
Msa0077300 MtrunA17_Chr1g0155761 36.494 348 191 5 13 336 18 359 1.11e-75 239
Msa0077300 MtrunA17_Chr6g0483401 39.877 326 176 5 25 338 19 336 1.37e-75 237
Msa0077300 MtrunA17_Chr3g0114701 38.769 325 196 1 13 334 6 330 3.86e-72 229
Msa0077300 MtrunA17_Chr6g0463971 37.322 351 204 7 1 340 1 346 6.92e-71 225
Msa0077300 MtrunA17_Chr8g0334761 38.462 338 200 4 15 345 9 345 6.71e-70 223
Msa0077300 MtrunA17_Chr2g0278541 38.485 330 191 6 15 334 11 338 4.80e-68 219
Msa0077300 MtrunA17_Chr2g0290611 38.416 341 202 7 10 345 6 343 5.68e-68 218
Msa0077300 MtrunA17_Chr3g0129601 35.866 329 206 2 11 335 24 351 6.47e-67 215
Msa0077300 MtrunA17_Chr1g0164831 33.913 345 218 6 15 357 2 338 1.32e-65 211
Msa0077300 MtrunA17_Chr2g0278551 35.277 343 212 7 11 346 7 346 1.52e-63 207
Msa0077300 MtrunA17_Chr2g0278561 34.412 340 212 4 10 343 9 343 1.64e-63 207
Msa0077300 MtrunA17_Chr8g0334771 37.536 349 209 6 17 357 11 358 5.97e-63 205
Msa0077300 MtrunA17_Chr2g0332001 35.061 328 207 3 7 331 29 353 2.74e-62 204
Msa0077300 MtrunA17_Chr2g0284261 33.540 322 212 2 13 334 5 324 3.05e-62 203
Msa0077300 MtrunA17_Chr4g0050891 33.639 327 202 5 13 329 10 331 9.19e-62 201
Msa0077300 MtrunA17_Chr3g0126101 35.312 320 204 3 17 336 7 323 9.43e-62 201
Msa0077300 MtrunA17_Chr4g0059311 33.623 345 220 6 15 356 13 351 1.82e-61 201
Msa0077300 MtrunA17_Chr2g0310441 34.431 334 215 3 15 346 9 340 2.36e-61 201
Msa0077300 MtrunA17_Chr8g0383731 35.758 330 204 3 13 335 2 330 6.26e-60 196
Msa0077300 MtrunA17_Chr1g0177221 36.400 250 159 0 13 262 15 264 7.03e-59 191
Msa0077300 MtrunA17_Chr7g0271561 33.636 330 208 4 17 338 8 334 2.73e-58 193
Msa0077300 MtrunA17_Chr3g0129571 35.061 328 205 4 14 334 5 331 1.15e-57 191
Msa0077300 MtrunA17_Chr4g0073241 33.626 342 213 6 17 351 9 343 7.40e-57 189
Msa0077300 MtrunA17_Chr4g0050941 34.943 352 206 8 13 346 9 355 1.57e-56 189
Msa0077300 MtrunA17_Chr4g0050881 34.513 339 204 8 13 340 6 337 1.69e-56 187
Msa0077300 MtrunA17_Chr8g0334751 34.188 351 213 5 15 356 10 351 2.25e-55 185
Msa0077300 MtrunA17_Chr3g0129591 33.841 328 209 4 14 334 3 329 4.74e-54 181
Msa0077300 MtrunA17_Chr5g0413961 33.025 324 210 4 15 338 16 332 8.23e-54 181
Msa0077300 MtrunA17_Chr4g0051021 31.686 344 224 5 16 355 14 350 1.23e-53 181
Msa0077300 MtrunA17_Chr1g0160111 31.056 322 210 4 25 338 16 333 2.71e-53 180
Msa0077300 MtrunA17_Chr5g0408001 31.765 340 214 5 13 336 7 344 1.14e-47 166
Msa0077300 MtrunA17_Chr5g0407991 31.858 339 211 5 15 336 8 343 2.56e-47 164
Msa0077300 MtrunA17_Chr4g0050931 31.765 340 180 7 13 343 9 305 2.85e-47 162
Msa0077300 MtrunA17_Chr5g0407971 31.965 341 212 6 13 336 8 345 4.46e-47 164
Msa0077300 MtrunA17_Chr1g0150151 33.828 337 210 7 10 338 5 336 4.76e-47 163
Msa0077300 MtrunA17_Chr5g0408031 30.836 347 220 5 13 341 7 351 1.64e-46 162
Msa0077300 MtrunA17_Chr3g0126091 31.402 328 219 4 14 336 9 335 5.75e-46 160
Msa0077300 MtrunA17_Chr3g0129581 33.537 328 210 4 14 334 3 329 8.24e-45 157
Msa0077300 MtrunA17_Chr5g0408041 30.321 343 219 6 16 340 11 351 8.66e-45 158
Msa0077300 MtrunA17_Chr5g0413941 30.719 306 202 5 36 336 27 327 4.58e-44 155
Msa0077300 MtrunA17_Chr5g0428881 27.976 336 237 3 3 334 5 339 5.34e-44 155
Msa0077300 MtrunA17_Chr6g0451171 32.571 350 225 7 12 357 8 350 6.97e-44 155
Msa0077300 MtrunA17_Chr5g0413921 29.524 315 206 6 36 342 27 333 7.64e-43 152
Msa0077300 MtrunA17_Chr5g0413931 32.258 310 202 5 36 339 26 333 8.73e-43 152
Msa0077300 MtrunA17_Chr7g0227921 29.697 330 229 3 10 338 5 332 9.84e-42 149
Msa0077300 MtrunA17_Chr6g0451211 33.030 330 209 7 12 335 8 331 1.41e-41 149
Msa0077300 MtrunA17_Chr5g0441761 26.994 326 233 2 21 346 19 339 3.83e-41 147
Msa0077300 MtrunA17_Chr4g0051001 30.820 305 204 3 13 312 28 330 2.59e-40 144
Msa0077300 MtrunA17_Chr5g0413951 29.193 322 216 6 21 336 12 327 1.06e-39 143
Msa0077300 MtrunA17_Chr5g0404811 37.674 215 128 3 126 336 3 215 1.65e-39 140
Msa0077300 MtrunA17_Chr6g0451201 31.902 326 213 6 12 335 8 326 2.95e-38 140
Msa0077300 MtrunA17_Chr8g0369841 28.994 338 222 5 15 336 8 343 4.33e-38 140
Msa0077300 MtrunA17_Chr3g0084091 29.545 264 165 3 91 343 5 258 2.41e-36 133
Msa0077300 MtrunA17_Chr6g0451251 33.333 300 188 8 41 335 37 329 1.49e-35 133
Msa0077300 MtrunA17_Chr6g0451261 30.503 318 213 7 12 325 7 320 7.42e-35 135
Msa0077300 MtrunA17_Chr1g0211801 30.233 258 171 4 9 262 4 256 1.96e-34 127
Msa0077300 MtrunA17_Chr5g0429001 27.839 273 190 2 68 334 3 274 2.33e-31 120
Msa0077300 MtrunA17_Chr5g0399281 29.129 333 209 8 26 340 14 337 3.88e-30 118
Msa0077300 MtrunA17_Chr6g0451191 34.222 225 138 5 130 350 1 219 2.39e-29 113
Msa0077300 MtrunA17_Chr2g0278021 33.333 213 133 3 130 334 1 212 4.55e-29 113
Msa0077300 MtrunA17_Chr4g0056081 27.187 320 214 7 42 349 40 352 1.48e-28 114
Msa0077300 MtrunA17_Chr1g0172881 37.241 145 78 4 122 261 12 148 3.14e-25 99.8
Msa0077300 MtrunA17_Chr8g0334721 43.697 119 65 2 225 343 1 117 4.77e-25 99.8
Msa0077300 MtrunA17_Chr8g0334711 33.673 196 127 3 15 208 9 203 3.37e-23 95.9
Msa0077300 MtrunA17_Chr1g0182261 35.220 159 94 3 200 356 74 225 1.69e-22 94.7
Msa0077300 MtrunA17_Chr1g0211811 47.368 76 38 1 264 337 1 76 2.33e-18 79.0
Msa0077300 MtrunA17_Chr4g0050921 38.835 103 62 1 213 315 3 104 2.86e-17 77.4
Msa0077300 MtrunA17_Chr5g0404801 34.454 119 78 0 11 129 2 120 3.10e-17 77.0
Msa0077300 MtrunA17_Chr2g0278031 34.483 116 76 0 13 128 11 126 1.48e-16 75.5
Msa0077300 MtrunA17_Chr8g0334731 35.135 148 92 3 65 208 40 187 1.99e-16 77.0
Msa0077300 MtrunA17_Chr1g0177231 46.753 77 41 0 260 336 26 102 2.88e-15 71.6
Msa0077300 MtrunA17_Chr1g0172891 42.857 77 44 0 272 348 16 92 2.50e-14 68.9
Msa0077300 MtrunA17_Chr4g0050911 28.244 131 80 3 92 210 7 135 7.90e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0077300 AT5G07050.1 43.072 332 177 4 16 337 17 346 8.77e-89 273
Msa0077300 AT2G39510.1 40.597 335 198 1 13 346 4 338 1.40e-87 269
Msa0077300 AT2G37460.1 40.062 322 192 1 13 334 11 331 7.56e-87 267
Msa0077300 AT4G08300.1 38.757 338 202 3 13 346 7 343 8.56e-83 256
Msa0077300 AT1G44800.1 39.941 338 197 4 13 346 7 342 3.36e-81 252
Msa0077300 AT2G40900.1 37.805 328 199 2 9 336 3 325 2.88e-80 251
Msa0077300 AT1G75500.1 37.151 358 209 4 11 357 14 366 3.85e-79 248
Msa0077300 AT1G75500.2 37.151 358 209 4 11 357 14 366 3.85e-79 248
Msa0077300 AT3G18200.2 38.235 340 206 3 2 340 20 356 6.50e-77 242
Msa0077300 AT5G13670.1 37.048 332 196 4 12 335 3 329 2.39e-76 240
Msa0077300 AT1G21890.1 36.119 335 203 2 13 337 7 340 1.15e-75 239
Msa0077300 AT4G08290.1 37.500 328 201 3 14 338 10 336 3.67e-75 237
Msa0077300 AT3G56620.1 38.650 326 194 3 9 333 3 323 9.73e-75 236
Msa0077300 AT2G37450.2 37.346 324 181 2 13 336 5 306 6.52e-70 222
Msa0077300 AT1G43650.1 35.736 333 211 2 14 346 4 333 5.66e-69 220
Msa0077300 AT4G30420.2 35.393 356 208 5 13 352 5 354 4.15e-67 216
Msa0077300 AT1G21890.2 35.161 310 190 2 13 312 7 315 5.84e-65 210
Msa0077300 AT4G30420.1 35.143 350 205 5 19 352 1 344 2.70e-64 209
Msa0077300 AT5G64700.1 34.795 342 214 3 16 349 7 347 3.75e-64 208
Msa0077300 AT3G30340.1 34.146 328 213 2 13 338 7 333 4.83e-63 205
Msa0077300 AT3G53210.1 34.848 330 198 5 16 336 9 330 1.79e-62 204
Msa0077300 AT1G09380.1 32.308 325 214 2 13 332 4 327 9.08e-61 200
Msa0077300 AT2G37450.3 40.000 235 140 1 102 336 1 234 8.68e-55 181
Msa0077300 AT3G18200.1 40.870 230 130 4 113 340 1 226 2.89e-53 176
Msa0077300 AT4G01440.1 29.154 319 224 2 14 331 7 324 7.41e-52 176
Msa0077300 AT1G11450.3 29.825 342 238 2 11 351 10 350 6.78e-49 168
Msa0077300 AT5G40210.1 31.579 304 190 5 36 338 32 318 4.70e-48 166
Msa0077300 AT1G68170.2 30.422 332 224 4 10 336 6 335 7.34e-48 166
Msa0077300 AT1G01070.1 29.885 348 230 6 9 349 8 348 1.85e-47 165
Msa0077300 AT4G08290.2 34.661 251 160 3 14 261 10 259 1.99e-46 160
Msa0077300 AT1G68170.1 30.841 321 215 4 21 336 7 325 4.33e-46 161
Msa0077300 AT1G11450.5 31.457 302 205 2 51 351 6 306 1.35e-45 158
Msa0077300 AT4G01440.3 27.667 300 215 2 14 312 7 305 1.16e-44 156
Msa0077300 AT2G37450.1 39.423 208 125 1 129 336 61 267 1.39e-44 155
Msa0077300 AT1G11450.2 31.208 298 203 2 55 351 3 299 5.36e-44 154
Msa0077300 AT3G53210.2 34.118 255 151 5 91 336 27 273 6.08e-44 154
Msa0077300 AT1G70260.1 27.119 354 226 4 13 346 8 349 1.15e-43 155
Msa0077300 AT3G28050.1 30.523 344 221 9 19 356 17 348 1.30e-43 154
Msa0077300 AT4G01430.1 27.920 351 244 7 13 356 5 353 2.55e-43 154
Msa0077300 AT1G11460.1 29.394 330 230 3 9 336 8 336 2.71e-43 153
Msa0077300 AT1G43650.2 33.468 248 162 2 14 261 4 248 1.82e-42 149
Msa0077300 AT4G28040.1 26.901 342 236 5 13 351 4 334 2.73e-42 151
Msa0077300 AT4G28040.5 26.901 342 236 5 13 351 4 334 2.73e-42 151
Msa0077300 AT4G28040.3 26.901 342 236 5 13 351 4 334 2.73e-42 151
Msa0077300 AT4G28040.4 26.901 342 236 5 13 351 4 334 2.73e-42 151
Msa0077300 AT4G28040.2 26.901 342 236 5 13 351 4 334 2.73e-42 151
Msa0077300 AT1G25270.1 29.448 326 224 3 15 336 1 324 3.97e-42 150
Msa0077300 AT5G64700.2 33.858 254 159 3 16 261 7 259 5.88e-42 147
Msa0077300 AT5G40230.1 31.579 304 199 6 41 338 45 345 8.63e-41 147
Msa0077300 AT1G60050.1 27.429 350 236 6 17 351 13 359 3.66e-40 145
Msa0077300 AT3G28130.2 29.755 326 217 6 21 339 17 337 4.06e-40 145
Msa0077300 AT3G53210.3 32.411 253 153 4 16 263 9 248 8.87e-40 142
Msa0077300 AT5G40240.1 29.605 304 205 5 41 338 44 344 3.62e-39 143
Msa0077300 AT5G40240.3 29.605 304 205 5 41 338 44 344 3.62e-39 143
Msa0077300 AT5G40240.2 29.605 304 205 5 41 338 58 358 5.58e-39 142
Msa0077300 AT5G45370.2 28.358 335 222 5 18 336 21 353 2.29e-38 141
Msa0077300 AT5G45370.4 28.358 335 222 5 18 336 21 353 2.29e-38 141
Msa0077300 AT5G45370.1 28.483 323 213 4 18 336 21 329 2.35e-38 140
Msa0077300 AT3G28130.5 32.245 245 160 3 99 339 9 251 1.77e-37 136
Msa0077300 AT3G28130.1 32.245 245 160 3 99 339 9 251 1.77e-37 136
Msa0077300 AT1G11450.4 28.713 303 214 2 11 312 10 311 2.28e-37 137
Msa0077300 AT1G01070.2 30.952 294 189 6 63 349 15 301 2.66e-37 137
Msa0077300 AT3G28100.1 29.114 316 213 7 26 336 26 335 9.97e-37 136
Msa0077300 AT4G24980.1 28.477 302 150 6 34 335 4 239 1.74e-36 133
Msa0077300 AT4G01440.5 30.841 214 146 2 119 331 4 216 5.67e-36 131
Msa0077300 AT4G01440.2 30.841 214 146 2 119 331 4 216 5.67e-36 131
Msa0077300 AT3G28070.2 30.677 251 168 4 99 344 7 256 4.26e-35 129
Msa0077300 AT4G15540.1 28.824 340 224 6 1 336 1 326 4.46e-35 131
Msa0077300 AT4G15540.2 28.824 340 224 6 1 336 1 326 4.46e-35 131
Msa0077300 AT3G30340.2 30.556 252 172 2 13 262 7 257 6.21e-35 129
Msa0077300 AT3G28070.1 30.677 251 168 4 99 344 99 348 3.54e-34 129
Msa0077300 AT3G28080.1 28.819 288 194 7 65 344 62 346 6.00e-34 129
Msa0077300 AT3G28080.5 29.200 250 168 5 102 344 10 257 7.44e-34 126
Msa0077300 AT3G28080.7 29.200 250 168 5 102 344 10 257 7.44e-34 126
Msa0077300 AT3G28080.8 29.200 250 168 5 102 344 10 257 7.44e-34 126
Msa0077300 AT3G28080.6 29.200 250 168 5 102 344 10 257 7.44e-34 126
Msa0077300 AT4G01430.2 26.885 305 217 5 13 312 5 308 1.48e-33 127
Msa0077300 AT1G11450.1 30.588 255 175 2 98 351 5 258 1.72e-33 125
Msa0077300 AT3G28080.2 29.200 250 168 5 102 344 91 338 4.29e-33 126
Msa0077300 AT3G45870.1 28.916 332 216 6 13 329 8 334 7.91e-32 123
Msa0077300 AT4G01450.2 27.864 323 221 3 14 332 7 321 9.66e-32 123
Msa0077300 AT4G19185.1 27.219 338 222 6 18 336 22 354 2.39e-31 122
Msa0077300 AT4G01450.1 26.733 303 210 3 14 312 7 301 6.57e-31 120
Msa0077300 AT4G01440.4 27.344 256 172 4 14 262 7 255 6.57e-31 118
Msa0077300 AT5G47470.2 28.053 303 205 5 42 338 83 378 4.24e-30 119
Msa0077300 AT5G47470.3 28.053 303 205 5 42 338 83 378 5.17e-30 118
Msa0077300 AT5G47470.1 28.053 303 205 5 42 338 55 350 5.41e-30 118
Msa0077300 AT3G28130.3 31.776 214 140 3 130 339 1 212 3.35e-29 113
Msa0077300 AT3G28070.5 30.455 220 147 4 130 344 1 219 4.57e-27 107
Msa0077300 AT3G28070.3 30.841 214 144 3 102 312 102 314 2.54e-26 107
Msa0077300 AT3G45870.8 27.937 315 207 6 13 312 8 317 2.86e-26 107
Msa0077300 AT3G28080.9 28.378 222 150 5 130 344 1 220 9.25e-26 103
Msa0077300 AT4G01450.4 27.801 241 162 3 14 250 7 239 7.59e-25 102
Msa0077300 AT5G45370.6 27.888 251 163 5 102 336 3 251 9.90e-24 99.4
Msa0077300 AT5G45370.5 27.888 251 163 5 102 336 3 251 9.90e-24 99.4
Msa0077300 AT4G19185.2 27.027 259 165 6 97 336 23 276 1.25e-23 100
Msa0077300 AT4G16620.1 25.915 328 220 6 24 338 19 336 2.38e-23 99.8
Msa0077300 AT4G01450.3 27.928 222 149 2 14 231 7 221 5.08e-23 96.7
Msa0077300 AT3G28100.3 29.583 240 159 6 26 260 26 260 1.03e-20 90.9
Msa0077300 AT4G01430.3 28.899 218 147 6 145 356 1 216 1.79e-20 89.4
Msa0077300 AT3G28130.4 28.112 249 168 5 21 262 17 261 6.91e-20 88.6
Msa0077300 AT3G45870.9 28.821 229 151 3 13 229 8 236 8.69e-18 82.8
Msa0077300 AT3G45870.5 29.279 222 137 6 123 329 43 259 2.53e-17 82.0
Msa0077300 AT5G45370.3 26.577 222 145 5 131 336 74 293 9.36e-17 80.5
Msa0077300 AT4G16620.4 26.104 249 162 5 102 338 1 239 3.52e-16 77.8
Msa0077300 AT4G16620.2 26.104 249 162 5 102 338 1 239 3.52e-16 77.8
Msa0077300 AT3G45870.2 30.769 143 91 3 190 329 46 183 2.05e-14 72.4
Msa0077300 AT3G28060.1 32.432 111 74 1 202 312 45 154 1.26e-13 68.2
Msa0077300 AT3G28100.2 32.374 139 89 3 26 163 26 160 1.18e-12 67.8
Msa0077300 AT3G45870.6 27.805 205 128 6 123 312 43 242 2.62e-12 66.6
Msa0077300 AT3G45870.7 27.805 205 128 6 123 312 43 242 2.62e-12 66.6
Msa0077300 AT3G28130.6 28.767 146 98 3 21 163 17 159 1.22e-11 63.9

Find 82 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTTGTGGTCCATAATATA+TGG 0.217089 1_2:-54176465 Msa0077300:CDS
TGTTGATCTACATTCAATTA+TGG 0.230717 1_2:-54176327 Msa0077300:CDS
ATATTTGTGCTTTCTTTGTT+AGG 0.235092 1_2:-54177446 Msa0077300:CDS
TATCAACGAGAGTGGATTAA+AGG 0.268996 1_2:-54176770 Msa0077300:CDS
TGGGATTTGTAGGTTTATTC+TGG 0.273990 1_2:-54175822 Msa0077300:three_prime_UTR
ATGTAACTGATTTGTCTAAA+TGG 0.284667 1_2:+54175689 None:intergenic
AAACCTAACCCCATATATTA+TGG 0.288350 1_2:+54176455 None:intergenic
CATATATCCTCTATTGTATT+GGG 0.306547 1_2:-54178179 None:intergenic
TTCAGAGATAAAATATAAAA+AGG 0.306735 1_2:+54177988 None:intergenic
TCGTAATTCTTTAGCATCAT+AGG 0.313102 1_2:-54176052 Msa0077300:intron
ATTTCAAGACCAAAGTTAAA+AGG 0.321589 1_2:-54175964 Msa0077300:CDS
CCATATATCCTCTATTGTAT+TGG 0.327582 1_2:-54178180 None:intergenic
AACCCTTCAGCATGGATTAT+AGG 0.328579 1_2:-54176498 Msa0077300:CDS
AAACATCATCACTAAACTTA+TGG 0.354829 1_2:-54178092 Msa0077300:exon
TCCTCTTTGTACTATATTTG+TGG 0.357975 1_2:-54176263 Msa0077300:CDS
GAATAAAATTTGCACATCAA+TGG 0.367395 1_2:-54175842 Msa0077300:three_prime_UTR
TGCCACAAGTTTCTCATAAC+AGG 0.377701 1_2:+54176829 None:intergenic
TGCACATCAATGGGATTTGT+AGG 0.379576 1_2:-54175832 Msa0077300:three_prime_UTR
AATAAAATTTGCACATCAAT+GGG 0.381793 1_2:-54175841 Msa0077300:three_prime_UTR
AGGTGCATTTATTGTGATCA+TGG 0.389621 1_2:-54176032 Msa0077300:CDS
ACTTGTGGTCCATAATATAT+GGG 0.400807 1_2:-54176464 Msa0077300:CDS
GGTTGAATAATATGGATTAG+AGG 0.401678 1_2:+54176805 None:intergenic
TATTAATGACAGGGAATTGT+TGG 0.403386 1_2:-54176358 Msa0077300:intron
GGGTTTGTATCTGTTGCTGT+GGG 0.425250 1_2:-54176011 Msa0077300:CDS
TATTTGTGCTTTCTTTGTTA+GGG 0.426849 1_2:-54177445 Msa0077300:intron
ACCTATCGGCATGTTCTCGC+CGG 0.429528 1_2:-54177926 Msa0077300:CDS
CCTATCGGCATGTTCTCGCC+GGG 0.430648 1_2:-54177925 Msa0077300:CDS
TTCTATATGGTACATTATGC+AGG 0.432883 1_2:-54176713 Msa0077300:intron
CTCAATTATCACTCACTACA+TGG 0.439857 1_2:-54176578 Msa0077300:CDS
ATCAACGAGAGTGGATTAAA+GGG 0.446252 1_2:-54176769 Msa0077300:CDS
AAAAGAACAAGTGTCAGCTT+TGG 0.474816 1_2:+54177476 None:intergenic
TCTGAATCTTCCTTCAATCA+TGG 0.477623 1_2:-54177971 Msa0077300:CDS
TGGGTTTGTATCTGTTGCTG+TGG 0.482889 1_2:-54176012 Msa0077300:CDS
GTTCAAGCCACACCTTATCA+TGG 0.485715 1_2:-54178032 Msa0077300:CDS
GGGACCTTGATATCCTTAGC+TGG 0.486715 1_2:-54176880 Msa0077300:CDS
CTCTCAAGAAAGTATGCAAA+AGG 0.494726 1_2:+54177890 None:intergenic
AACACCAGCTAAGGATATCA+AGG 0.497528 1_2:+54176876 None:intergenic
GTTTGTATCTGTTGCTGTGG+GGG 0.500052 1_2:-54176009 Msa0077300:CDS
AACATGGAAGCAAGAAATGT+TGG 0.500297 1_2:+54177293 None:intergenic
GTAGTGAGTGATAATTGAGC+AGG 0.509226 1_2:+54176582 None:intergenic
CTTATGTTTATGTTACCTAT+CGG 0.512879 1_2:-54177940 Msa0077300:CDS
GGTGCATTTATTGTGATCAT+GGG 0.513185 1_2:-54176031 Msa0077300:CDS
TGACAGGGAATTGTTGGTGG+TGG 0.516639 1_2:-54176352 Msa0077300:intron
ATACTAGCATACTTCGTCCT+TGG 0.517108 1_2:-54176238 Msa0077300:CDS
TTGACTTGAATGCAGGATTG+AGG 0.518118 1_2:-54176944 Msa0077300:intron
TTTCACCATGACACTATACA+AGG 0.524518 1_2:-54176854 Msa0077300:CDS
ATTTAGACAAATCAGTTACA+TGG 0.531067 1_2:-54175687 Msa0077300:three_prime_UTR
TCATTATTGCAGTGGCACTC+AGG 0.535841 1_2:-54177247 Msa0077300:intron
ACTAGAACCCAATACAATAG+AGG 0.538638 1_2:+54178172 None:intergenic
ATTTGTCTAAATGGCCACAT+AGG 0.538743 1_2:+54175698 None:intergenic
ATATAAGAAATTCGAATCGA+TGG 0.540403 1_2:+54175607 None:intergenic
CTTGGGCTAAAACCATGATA+AGG 0.551206 1_2:+54178020 None:intergenic
CACTGCAATAATGAAGGTAA+TGG 0.555306 1_2:+54177256 None:intergenic
AACTCTTTGTATACATGTAG+AGG 0.556101 1_2:+54178052 None:intergenic
AGTCCTATAATCCATGCTGA+AGG 0.557310 1_2:+54176495 None:intergenic
CATTACCTTCATTATTGCAG+TGG 0.559816 1_2:-54177255 Msa0077300:CDS
AGTTCTTCTATCAACGAGAG+TGG 0.559959 1_2:-54176778 Msa0077300:CDS
CGATAGGTAACATAAACATA+AGG 0.561067 1_2:+54177941 None:intergenic
TTATATATATAGTGGGAATG+TGG 0.561311 1_2:-54178141 Msa0077300:five_prime_UTR
CATGGTGAAAACACCAGCTA+AGG 0.566514 1_2:+54176867 None:intergenic
GAGTGCCACTGCAATAATGA+AGG 0.571106 1_2:+54177250 None:intergenic
AGGACTCATTCCATGATTGA+AGG 0.576140 1_2:+54177961 None:intergenic
GGTTTGTATCTGTTGCTGTG+GGG 0.597926 1_2:-54176010 Msa0077300:CDS
TAATGACAGGGAATTGTTGG+TGG 0.600813 1_2:-54176355 Msa0077300:intron
GCCACAAATATAGTACAAAG+AGG 0.604972 1_2:+54176262 None:intergenic
AGGTCCCTTGTATAGTGTCA+TGG 0.606603 1_2:+54176849 None:intergenic
GTCCTATAATCCATGCTGAA+GGG 0.614393 1_2:+54176496 None:intergenic
AACATAATAACCCTTCAGCA+TGG 0.615122 1_2:-54176506 Msa0077300:CDS
CTTGTGGTCCATAATATATG+GGG 0.619780 1_2:-54176463 Msa0077300:intron
GGGGAAAGAAGGTGACAAAG+AGG 0.625341 1_2:-54175990 Msa0077300:CDS
GGAAGCTATAGTGTTGAACA+TGG 0.625781 1_2:+54177277 None:intergenic
TAGGACTCAACATTGACTTG+TGG 0.640232 1_2:-54176479 Msa0077300:CDS
CCCGGCGAGAACATGCCGAT+AGG 0.651355 1_2:+54177925 None:intergenic
TAGTGAGTGATAATTGAGCA+GGG 0.654187 1_2:+54176583 None:intergenic
AAAATATGGCAGGATCACCA+TGG 0.660749 1_2:-54177354 Msa0077300:intron
CTATCGGCATGTTCTCGCCG+GGG 0.671878 1_2:-54177924 Msa0077300:CDS
GCTAAAACCATGATAAGGTG+TGG 0.672505 1_2:+54178025 None:intergenic
AAAAGGAAACATCACAACCC+CGG 0.676563 1_2:+54177907 None:intergenic
TTCACCATGACACTATACAA+GGG 0.685500 1_2:-54176853 Msa0077300:CDS
AAAGTACAAATTGAGTGCCA+TGG 0.689345 1_2:+54177337 None:intergenic
AATGTACCATATAGAAAACG+TGG 0.726303 1_2:+54176720 None:intergenic
GACCTGTTATGAGAAACTTG+TGG 0.744585 1_2:-54176831 Msa0077300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAAAATAGAAATTTTATA+AGG + chr1_2:54176598-54176617 None:intergenic 10.0%
!!! AAATTTCTATTTTTTGTTTT+TGG - chr1_2:54176602-54176621 Msa0077300:CDS 10.0%
!! TAATGTTAGAAATTTATATT+TGG + chr1_2:54177381-54177400 None:intergenic 10.0%
!!! ACTTTTTCTTATATATATAG+TGG - chr1_2:54175626-54175645 Msa0077300:three_prime_UTR 15.0%
!!! CTTTTTCTTATATATATAGT+GGG - chr1_2:54175627-54175646 Msa0077300:three_prime_UTR 15.0%
!! TTCAGAGATAAAATATAAAA+AGG + chr1_2:54175790-54175809 None:intergenic 15.0%
!!! ATGTTTTTAGTTTTGTAATT+TGG + chr1_2:54176653-54176672 None:intergenic 15.0%
!!! CAAATTTTATTCTAAATACT+TGG + chr1_2:54177925-54177944 None:intergenic 15.0%
!! ATGTATGTTATTACATTGTA+TGG - chr1_2:54176369-54176388 Msa0077300:intron 20.0%
!!! GTATAATTTTAAAATATGGC+AGG - chr1_2:54176411-54176430 Msa0077300:intron 20.0%
!!! TTTTAGTTTTGTAATTTGGA+AGG + chr1_2:54176649-54176668 None:intergenic 20.0%
!!! AAAATCTAACCTATTGATTT+CGG - chr1_2:54176774-54176793 Msa0077300:CDS 20.0%
!!! AACTTTTTGGTTGAATAATA+TGG + chr1_2:54176981-54177000 None:intergenic 20.0%
!! TAAAAACTAGTTATGAGTTA+AGG + chr1_2:54177094-54177113 None:intergenic 20.0%
!! AACTCTAATATCTTTGTAAT+TGG - chr1_2:54177141-54177160 Msa0077300:intron 20.0%
!! AATCATTCAATATTAATGAC+AGG - chr1_2:54177407-54177426 Msa0077300:intron 20.0%
!! ATCATTCAATATTAATGACA+GGG - chr1_2:54177408-54177427 Msa0077300:intron 20.0%
!! TAACTTTAGTTGGAAATTAA+AGG - chr1_2:54177883-54177902 Msa0077300:intron 20.0%
!! AATAAAATTTGCACATCAAT+GGG - chr1_2:54177934-54177953 Msa0077300:CDS 20.0%
!! TTATATATATAGTGGGAATG+TGG - chr1_2:54175634-54175653 Msa0077300:three_prime_UTR 25.0%
! AAACATCATCACTAAACTTA+TGG - chr1_2:54175683-54175702 Msa0077300:three_prime_UTR 25.0%
! CTTATGTTTATGTTACCTAT+CGG - chr1_2:54175835-54175854 Msa0077300:three_prime_UTR 25.0%
! ATATTTGTGCTTTCTTTGTT+AGG - chr1_2:54176329-54176348 Msa0077300:CDS 25.0%
! TATTTGTGCTTTCTTTGTTA+GGG - chr1_2:54176330-54176349 Msa0077300:CDS 25.0%
! TATTACATTGTATGGAAGTT+TGG - chr1_2:54176377-54176396 Msa0077300:intron 25.0%
! ATCTTTGTAATTGGAAATTC+AGG - chr1_2:54177150-54177169 Msa0077300:intron 25.0%
! TGTTGATCTACATTCAATTA+TGG - chr1_2:54177448-54177467 Msa0077300:CDS 25.0%
! TTAAATACAGTGACAAAAAC+AGG + chr1_2:54177492-54177511 None:intergenic 25.0%
! TACTATGAATATGTAGTAAG+TGG - chr1_2:54177634-54177653 Msa0077300:intron 25.0%
! ATTTCAAGACCAAAGTTAAA+AGG - chr1_2:54177811-54177830 Msa0077300:intron 25.0%
! ATATAGCATGTAACTTTAGT+TGG - chr1_2:54177873-54177892 Msa0077300:intron 25.0%
! GAATAAAATTTGCACATCAA+TGG - chr1_2:54177933-54177952 Msa0077300:CDS 25.0%
!!! ATTCGTTTTTGTGTAAAACA+AGG - chr1_2:54177985-54178004 Msa0077300:CDS 25.0%
!!! ATGTAACTGATTTGTCTAAA+TGG + chr1_2:54178089-54178108 None:intergenic 25.0%
! ATTTAGACAAATCAGTTACA+TGG - chr1_2:54178088-54178107 Msa0077300:exon 25.0%
! ATATAAGAAATTCGAATCGA+TGG + chr1_2:54178171-54178190 None:intergenic 25.0%
AACTCTTTGTATACATGTAG+AGG + chr1_2:54175726-54175745 None:intergenic 30.0%
! TAAAAAGGTATAGCCAACTT+GGG + chr1_2:54175775-54175794 None:intergenic 30.0%
! ATAAAAAGGTATAGCCAACT+TGG + chr1_2:54175776-54175795 None:intergenic 30.0%
CGATAGGTAACATAAACATA+AGG + chr1_2:54175837-54175856 None:intergenic 30.0%
! CAAAGTTCATAGTTTGTGTT+TGG - chr1_2:54175937-54175956 Msa0077300:CDS 30.0%
!! TTAATCTTCTAGCTTAACCA+AGG + chr1_2:54176124-54176143 None:intergenic 30.0%
GTTCGCAATTCTTGATATAA+TGG - chr1_2:54176550-54176569 Msa0077300:CDS 30.0%
ATAAATGCTCCGAAATCAAT+AGG + chr1_2:54176786-54176805 None:intergenic 30.0%
GTAGCATAGCAAAAATTCTT+GGG - chr1_2:54176875-54176894 Msa0077300:CDS 30.0%
!! GGTTGAATAATATGGATTAG+AGG + chr1_2:54176973-54176992 None:intergenic 30.0%
!! TCGTTGATAGAAGAACTTTT+TGG + chr1_2:54176994-54177013 None:intergenic 30.0%
AATGTACCATATAGAAAACG+TGG + chr1_2:54177058-54177077 None:intergenic 30.0%
TTCTATATGGTACATTATGC+AGG - chr1_2:54177062-54177081 Msa0077300:intron 30.0%
ACTTGTGGTCCATAATATAT+GGG - chr1_2:54177311-54177330 Msa0077300:CDS 30.0%
AAACCTAACCCCATATATTA+TGG + chr1_2:54177323-54177342 None:intergenic 30.0%
TATTAATGACAGGGAATTGT+TGG - chr1_2:54177417-54177436 Msa0077300:intron 30.0%
TCCTCTTTGTACTATATTTG+TGG - chr1_2:54177512-54177531 Msa0077300:intron 30.0%
CTTGGTGAAAAACTTTATCT+GGG - chr1_2:54177555-54177574 Msa0077300:intron 30.0%
TCGTAATTCTTTAGCATCAT+AGG - chr1_2:54177723-54177742 Msa0077300:intron 30.0%
!!! TTTGTACTGCCTTTTAACTT+TGG + chr1_2:54177823-54177842 None:intergenic 30.0%
GTTAAAAGGCAGTACAAAAA+TGG - chr1_2:54177825-54177844 Msa0077300:intron 30.0%
TTGGAAATTAAAGGTAGTAG+AGG - chr1_2:54177892-54177911 Msa0077300:CDS 30.0%
CCACATAGGTTATAAAATTC+TGG + chr1_2:54178066-54178085 None:intergenic 30.0%
! CCAGAATTTTATAACCTATG+TGG - chr1_2:54178063-54178082 Msa0077300:CDS 30.0%
TCTGAATCTTCCTTCAATCA+TGG - chr1_2:54175804-54175823 Msa0077300:three_prime_UTR 35.0%
! CTCTCAAGAAAGTATGCAAA+AGG + chr1_2:54175888-54175907 None:intergenic 35.0%
! CTTTTGCATACTTTCTTGAG+AGG - chr1_2:54175886-54175905 Msa0077300:intron 35.0%
! AATCATGATTGTAAGGCACT+CGG + chr1_2:54175963-54175982 None:intergenic 35.0%
AAAAGAACAAGTGTCAGCTT+TGG + chr1_2:54176302-54176321 None:intergenic 35.0%
! GCTGACACTTGTTCTTTTTA+TGG - chr1_2:54176304-54176323 Msa0077300:CDS 35.0%
AAAGTACAAATTGAGTGCCA+TGG + chr1_2:54176441-54176460 None:intergenic 35.0%
AACATGGAAGCAAGAAATGT+TGG + chr1_2:54176485-54176504 None:intergenic 35.0%
CACTGCAATAATGAAGGTAA+TGG + chr1_2:54176522-54176541 None:intergenic 35.0%
CATTACCTTCATTATTGCAG+TGG - chr1_2:54176520-54176539 Msa0077300:CDS 35.0%
AATTTGGAAGGACACAAAAG+TGG + chr1_2:54176637-54176656 None:intergenic 35.0%
CTAGATTCTTCAACGGATAT+TGG - chr1_2:54176709-54176728 Msa0077300:intron 35.0%
!! AGAATTTTTGCTATGCTACG+TGG + chr1_2:54176874-54176893 None:intergenic 35.0%
CGTAGCATAGCAAAAATTCT+TGG - chr1_2:54176874-54176893 Msa0077300:CDS 35.0%
TTTCACCATGACACTATACA+AGG - chr1_2:54176921-54176940 Msa0077300:CDS 35.0%
TTCACCATGACACTATACAA+GGG - chr1_2:54176922-54176941 Msa0077300:CDS 35.0%
!! TATCAACGAGAGTGGATTAA+AGG - chr1_2:54177005-54177024 Msa0077300:intron 35.0%
!! ATCAACGAGAGTGGATTAAA+GGG - chr1_2:54177006-54177025 Msa0077300:intron 35.0%
TAGTGAGTGATAATTGAGCA+GGG + chr1_2:54177195-54177214 None:intergenic 35.0%
CTCAATTATCACTCACTACA+TGG - chr1_2:54177197-54177216 Msa0077300:intron 35.0%
!! ACTACATGGATGTGCTTTTT+GGG - chr1_2:54177211-54177230 Msa0077300:intron 35.0%
AACATAATAACCCTTCAGCA+TGG - chr1_2:54177269-54177288 Msa0077300:CDS 35.0%
GACTTGTGGTCCATAATATA+TGG - chr1_2:54177310-54177329 Msa0077300:CDS 35.0%
CTTGTGGTCCATAATATATG+GGG - chr1_2:54177312-54177331 Msa0077300:CDS 35.0%
TTATGGTGCACTGAGAAAAA+AGG - chr1_2:54177465-54177484 Msa0077300:CDS 35.0%
TATGGTGCACTGAGAAAAAA+GGG - chr1_2:54177466-54177485 Msa0077300:CDS 35.0%
GCCACAAATATAGTACAAAG+AGG + chr1_2:54177516-54177535 None:intergenic 35.0%
! CCAGATAAAGTTTTTCACCA+AGG + chr1_2:54177557-54177576 None:intergenic 35.0%
CCTTGGTGAAAAACTTTATC+TGG - chr1_2:54177554-54177573 Msa0077300:intron 35.0%
AGGTGCATTTATTGTGATCA+TGG - chr1_2:54177743-54177762 Msa0077300:intron 35.0%
GGTGCATTTATTGTGATCAT+GGG - chr1_2:54177744-54177763 Msa0077300:intron 35.0%
TGGGATTTGTAGGTTTATTC+TGG - chr1_2:54177953-54177972 Msa0077300:CDS 35.0%
ATTTGTCTAAATGGCCACAT+AGG + chr1_2:54178080-54178099 None:intergenic 35.0%
GCTAAAACCATGATAAGGTG+TGG + chr1_2:54175753-54175772 None:intergenic 40.0%
CTTGGGCTAAAACCATGATA+AGG + chr1_2:54175758-54175777 None:intergenic 40.0%
!!! ATCATGGTTTTAGCCCAAGT+TGG - chr1_2:54175759-54175778 Msa0077300:three_prime_UTR 40.0%
AGGACTCATTCCATGATTGA+AGG + chr1_2:54175817-54175836 None:intergenic 40.0%
AAAAGGAAACATCACAACCC+CGG + chr1_2:54175871-54175890 None:intergenic 40.0%
TGCCTTACAATCATGATTCG+TGG - chr1_2:54175965-54175984 Msa0077300:CDS 40.0%
CACCACGAATCATGATTGTA+AGG + chr1_2:54175970-54175989 None:intergenic 40.0%
TCTTGTGAGATCCAAATGGA+CGG - chr1_2:54176038-54176057 Msa0077300:CDS 40.0%
CAGCAGCATTAAGACTCTTA+GGG + chr1_2:54176072-54176091 None:intergenic 40.0%
! TCTAGCTTAACCAAGGTTCT+GGG + chr1_2:54176117-54176136 None:intergenic 40.0%
!! TTCTAGCTTAACCAAGGTTC+TGG + chr1_2:54176118-54176137 None:intergenic 40.0%
AAAATATGGCAGGATCACCA+TGG - chr1_2:54176421-54176440 Msa0077300:intron 40.0%
! GGAAGCTATAGTGTTGAACA+TGG + chr1_2:54176501-54176520 None:intergenic 40.0%
GTGAGTGCTAGATTCTTCAA+CGG - chr1_2:54176702-54176721 Msa0077300:intron 40.0%
!! AAGAGAGTTGACTTGAATGC+AGG - chr1_2:54176824-54176843 Msa0077300:CDS 40.0%
! TTGACTTGAATGCAGGATTG+AGG - chr1_2:54176831-54176850 Msa0077300:CDS 40.0%
AACACCAGCTAAGGATATCA+AGG + chr1_2:54176902-54176921 None:intergenic 40.0%
GACCTGTTATGAGAAACTTG+TGG - chr1_2:54176944-54176963 Msa0077300:intron 40.0%
TGCCACAAGTTTCTCATAAC+AGG + chr1_2:54176949-54176968 None:intergenic 40.0%
AGTTCTTCTATCAACGAGAG+TGG - chr1_2:54176997-54177016 Msa0077300:intron 40.0%
! GTTGTGCCACGTTTTCTATA+TGG - chr1_2:54177049-54177068 Msa0077300:intron 40.0%
GTAGTGAGTGATAATTGAGC+AGG + chr1_2:54177196-54177215 None:intergenic 40.0%
! CACTACATGGATGTGCTTTT+TGG - chr1_2:54177210-54177229 Msa0077300:intron 40.0%
! AACCCTTCAGCATGGATTAT+AGG - chr1_2:54177277-54177296 Msa0077300:CDS 40.0%
GTCCTATAATCCATGCTGAA+GGG + chr1_2:54177282-54177301 None:intergenic 40.0%
AGTCCTATAATCCATGCTGA+AGG + chr1_2:54177283-54177302 None:intergenic 40.0%
!! TAGGACTCAACATTGACTTG+TGG - chr1_2:54177296-54177315 Msa0077300:CDS 40.0%
GGTCCATAATATATGGGGTT+AGG - chr1_2:54177317-54177336 Msa0077300:CDS 40.0%
TAATGACAGGGAATTGTTGG+TGG - chr1_2:54177420-54177439 Msa0077300:intron 40.0%
ATACTAGCATACTTCGTCCT+TGG - chr1_2:54177537-54177556 Msa0077300:intron 40.0%
GTGAAAAACTTTATCTGGGC+AGG - chr1_2:54177559-54177578 Msa0077300:intron 40.0%
AGGCAGTACAAAAATGGAGA+AGG - chr1_2:54177831-54177850 Msa0077300:intron 40.0%
CAGTACAAAAATGGAGAAGG+AGG - chr1_2:54177834-54177853 Msa0077300:intron 40.0%
TGCACATCAATGGGATTTGT+AGG - chr1_2:54177943-54177962 Msa0077300:CDS 40.0%
GTTCAAGCCACACCTTATCA+TGG - chr1_2:54175743-54175762 Msa0077300:three_prime_UTR 45.0%
TTGGATCTCACAAGACTCGA+GGG + chr1_2:54176033-54176052 None:intergenic 45.0%
TTTGGATCTCACAAGACTCG+AGG + chr1_2:54176034-54176053 None:intergenic 45.0%
! CGAGTCTTGTGAGATCCAAA+TGG - chr1_2:54176034-54176053 Msa0077300:CDS 45.0%
GCAGCAGCATTAAGACTCTT+AGG + chr1_2:54176073-54176092 None:intergenic 45.0%
! GAGTGCCACTGCAATAATGA+AGG + chr1_2:54176528-54176547 None:intergenic 45.0%
TCATTATTGCAGTGGCACTC+AGG - chr1_2:54176528-54176547 Msa0077300:CDS 45.0%
CATGGTGAAAACACCAGCTA+AGG + chr1_2:54176911-54176930 None:intergenic 45.0%
AGGTCCCTTGTATAGTGTCA+TGG + chr1_2:54176929-54176948 None:intergenic 45.0%
TGGGTTTGTATCTGTTGCTG+TGG - chr1_2:54177763-54177782 Msa0077300:intron 45.0%
GGGTTTGTATCTGTTGCTGT+GGG - chr1_2:54177764-54177783 Msa0077300:intron 45.0%
GGTTTGTATCTGTTGCTGTG+GGG - chr1_2:54177765-54177784 Msa0077300:intron 45.0%
GTTTGTATCTGTTGCTGTGG+GGG - chr1_2:54177766-54177785 Msa0077300:intron 45.0%
!!! ATTTGTATAATTTTAAAATA+TGG - chr1_2:54176407-54176426 Msa0077300:intron 5.0%
!! TAGTTTTGCAGTTGCGCACG+TGG + chr1_2:54176008-54176027 None:intergenic 50.0%
TCTGGGTTCGAATCCAGCAT+TGG + chr1_2:54176100-54176119 None:intergenic 50.0%
TGGATTCGAACCCAGAACCT+TGG - chr1_2:54176104-54176123 Msa0077300:intron 50.0%
GGGACCTTGATATCCTTAGC+TGG - chr1_2:54176895-54176914 Msa0077300:CDS 50.0%
! TGACAGGGAATTGTTGGTGG+TGG - chr1_2:54177423-54177442 Msa0077300:intron 50.0%
GGGGAAAGAAGGTGACAAAG+AGG - chr1_2:54177785-54177804 Msa0077300:intron 50.0%
ACCTATCGGCATGTTCTCGC+CGG - chr1_2:54175849-54175868 Msa0077300:three_prime_UTR 55.0%
TTGCGCACGTGGAGGATACT+CGG + chr1_2:54175997-54176016 None:intergenic 55.0%
! TTTTGCAGTTGCGCACGTGG+AGG + chr1_2:54176005-54176024 None:intergenic 55.0%
GGGAGAGTTTGCCGTCCATT+TGG + chr1_2:54176052-54176071 None:intergenic 55.0%
TGCTGCTGCATGTCCAATGC+TGG - chr1_2:54176084-54176103 Msa0077300:intron 55.0%
CTGTTGCTGTGGGGGAAAGA+AGG - chr1_2:54177774-54177793 Msa0077300:intron 55.0%
CCTATCGGCATGTTCTCGCC+GGG - chr1_2:54175850-54175869 Msa0077300:three_prime_UTR 60.0%
CTATCGGCATGTTCTCGCCG+GGG - chr1_2:54175851-54175870 Msa0077300:three_prime_UTR 60.0%
CCCGGCGAGAACATGCCGAT+AGG + chr1_2:54175853-54175872 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 54175604 54178193 54175604 ID=Msa0077300;Name=Msa0077300
chr1_2 mRNA 54175604 54178193 54175604 ID=Msa0077300-mRNA-1;Parent=Msa0077300;Name=Msa0077300-mRNA-1;_AED=0.30;_eAED=0.30;_QI=98|1|1|1|0.75|0.66|9|281|357
chr1_2 exon 54175604 54175720 54175604 ID=Msa0077300-mRNA-1:exon:16716;Parent=Msa0077300-mRNA-1
chr1_2 exon 54175741 54175885 54175741 ID=Msa0077300-mRNA-1:exon:16715;Parent=Msa0077300-mRNA-1
chr1_2 exon 54175906 54176060 54175906 ID=Msa0077300-mRNA-1:exon:16714;Parent=Msa0077300-mRNA-1
chr1_2 exon 54176217 54176368 54176217 ID=Msa0077300-mRNA-1:exon:16713;Parent=Msa0077300-mRNA-1
chr1_2 exon 54176464 54176625 54176464 ID=Msa0077300-mRNA-1:exon:16712;Parent=Msa0077300-mRNA-1
chr1_2 exon 54176714 54176951 54176714 ID=Msa0077300-mRNA-1:exon:16711;Parent=Msa0077300-mRNA-1
chr1_2 exon 54177248 54177364 54177248 ID=Msa0077300-mRNA-1:exon:16710;Parent=Msa0077300-mRNA-1
chr1_2 exon 54177446 54177508 54177446 ID=Msa0077300-mRNA-1:exon:16709;Parent=Msa0077300-mRNA-1
chr1_2 exon 54177890 54178193 54177890 ID=Msa0077300-mRNA-1:exon:16708;Parent=Msa0077300-mRNA-1
chr1_2 five_prime_UTR 54178096 54178193 54178096 ID=Msa0077300-mRNA-1:five_prime_utr;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54177890 54178095 54177890 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54177446 54177508 54177446 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54177248 54177364 54177248 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54176714 54176951 54176714 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54176464 54176625 54176464 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54176217 54176368 54176217 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 CDS 54175925 54176060 54175925 ID=Msa0077300-mRNA-1:cds;Parent=Msa0077300-mRNA-1
chr1_2 three_prime_UTR 54175906 54175924 54175906 ID=Msa0077300-mRNA-1:three_prime_utr;Parent=Msa0077300-mRNA-1
chr1_2 three_prime_UTR 54175741 54175885 54175741 ID=Msa0077300-mRNA-1:three_prime_utr;Parent=Msa0077300-mRNA-1
chr1_2 three_prime_UTR 54175604 54175720 54175604 ID=Msa0077300-mRNA-1:three_prime_utr;Parent=Msa0077300-mRNA-1
Gene Sequence

>Msa0077300

ATGGAGAAAAAAGTACTTTCTCCTCTACATGTATACAAAGAGTTCAAGCCACACCTTATCATGGTTTTAGCCCAAGTTGGCTATACCTTTTTATATTTTATCTCTGAATCTTCCTTCAATCATGGAATGAGTCCTTATGTTTATGTTACCTATCGGCATGTTCTCGCCGGGGTTGTGATGTTTCCTTTTGCATACTTTCTTGAGAGAAGTTTAAGACCAAAGCTGACACTTGTTCTTTTTATGGAAATATTTGTGCTTTCTTTGTTAGGGATCACCATGGCACTCAATTTGTACTTTGCAAGCTTAAAGTATACTTCTCCAACATTTCTTGCTTCCATGTTCAACACTATAGCTTCCATTACCTTCATTATTGCAGTGGCACTCAGGATTGAGGTTATTGATCTTAGAAATCCACGTAGCATAGCAAAAATTCTTGGGACCTTGATATCCTTAGCTGGTGTTTTCACCATGACACTATACAAGGGACCTGTTATGAGAAACTTGTGGCATCCTCTAATCCATATTATTCAACCAAAAAGTTCTTCTATCAACGAGAGTGGATTAAAGGGTTCAATTCTTACTGTCTCATGTTGTGCCACGTTTTCTATATGGTACATTATGCAGGCATCAACTCTAAAAAGATACCCTGCTCAATTATCACTCACTACATGGATGTGCTTTTTGGGAGCAGCACAATCAGCTATTTTCACAATAATAGTAGAACATAATAACCCTTCAGCATGGATTATAGGACTCAACATTGACTTGTGGTCCATAATATATGGGGGAATTGTTGGTGGTGGTTTGTTGATCTACATTCAATTATGGTGCACTGAGAAAAAAGGGCCTGTTTTTGTCACTGTATTTAATCCTCTTTGTACTATATTTGTGGCAATACTAGCATACTTCGTCCTTGGTGAAAAACTTTATCTGGGCAGCATCATAGGTGCATTTATTGTGATCATGGGTTTGTATCTGTTGCTGTGGGGGAAAGAAGGTGACAAAGAGGTTGATTTCAAGACCAAAGTTAAAAGGCAGTACAAAAATGGAGAAGGAGGCTTAGAAGAATGCTGA
Protein sequence

>Msa0077300

MEKKVLSPLHVYKEFKPHLIMVLAQVGYTFLYFISESSFNHGMSPYVYVTYRHVLAGVVMFPFAYFLERSLRPKLTLVLFMEIFVLSLLGITMALNLYFASLKYTSPTFLASMFNTIASITFIIAVALRIEVIDLRNPRSIAKILGTLISLAGVFTMTLYKGPVMRNLWHPLIHIIQPKSSSINESGLKGSILTVSCCATFSIWYIMQASTLKRYPAQLSLTTWMCFLGAAQSAIFTIIVEHNNPSAWIIGLNIDLWSIIYGGIVGGGLLIYIQLWCTEKKGPVFVTVFNPLCTIFVAILAYFVLGEKLYLGSIIGAFIVIMGLYLLLWGKEGDKEVDFKTKVKRQYKNGEGGLEEC*