AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0169730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0169730 MtrunA17_Chr1g0186561 95.798 357 14 1 1 356 1 357 0.0 664
Msa0169730 MtrunA17_Chr1g0186601 86.842 342 44 1 1 342 1 341 0.0 614
Msa0169730 MtrunA17_Chr1g0186571 79.832 357 67 3 1 356 1 353 0.0 582
Msa0169730 MtrunA17_Chr1g0186611 79.943 349 69 1 1 349 1 348 0.0 578
Msa0169730 MtrunA17_Chr1g0186641 79.213 356 61 3 1 356 1 343 0.0 576
Msa0169730 MtrunA17_Chr1g0171301 75.350 357 85 2 1 356 1 355 0.0 547
Msa0169730 MtrunA17_Chr1g0184221 52.890 346 143 4 11 342 11 350 6.00e-128 371
Msa0169730 MtrunA17_Chr7g0255561 44.277 332 175 4 12 336 10 338 1.41e-93 284
Msa0169730 MtrunA17_Chr7g0255581 44.277 332 175 4 12 336 10 338 1.41e-93 284
Msa0169730 MtrunA17_Chr7g0263101 44.545 330 174 5 12 336 9 334 3.62e-92 280
Msa0169730 MtrunA17_Chr1g0182291 42.547 322 184 1 13 334 12 332 1.72e-90 275
Msa0169730 MtrunA17_Chr1g0160171 41.389 360 185 4 13 355 13 363 2.24e-89 273
Msa0169730 MtrunA17_Chr3g0114691 38.372 344 200 3 13 349 17 355 7.77e-85 262
Msa0169730 MtrunA17_Chr1g0160181 40.854 328 191 1 12 336 14 341 1.11e-84 261
Msa0169730 MtrunA17_Chr3g0114731 40.798 326 190 3 16 340 18 341 3.13e-83 258
Msa0169730 MtrunA17_Chr3g0081421 42.202 327 178 5 16 334 17 340 5.48e-82 255
Msa0169730 MtrunA17_Chr3g0081511 41.284 327 188 3 12 335 13 338 2.22e-80 251
Msa0169730 MtrunA17_Chr3g0142131 39.941 338 188 3 13 336 17 353 5.38e-80 249
Msa0169730 MtrunA17_Chr8g0354121 40.351 342 182 8 12 338 9 343 2.79e-79 248
Msa0169730 MtrunA17_Chr3g0114711 34.530 362 201 3 16 341 14 375 2.39e-76 240
Msa0169730 MtrunA17_Chr6g0483401 39.877 326 176 5 25 338 19 336 1.23e-75 237
Msa0169730 MtrunA17_Chr1g0155761 36.494 348 191 5 13 336 18 359 1.46e-75 238
Msa0169730 MtrunA17_Chr3g0114701 39.077 325 195 1 13 334 6 330 2.28e-72 229
Msa0169730 MtrunA17_Chr6g0463971 36.986 365 211 8 1 354 1 357 5.77e-72 228
Msa0169730 MtrunA17_Chr8g0334761 38.462 338 200 4 15 345 9 345 1.31e-69 222
Msa0169730 MtrunA17_Chr2g0278541 38.485 330 191 6 15 334 11 338 5.95e-68 218
Msa0169730 MtrunA17_Chr2g0290611 38.416 341 202 7 10 345 6 343 6.06e-68 218
Msa0169730 MtrunA17_Chr3g0129601 34.571 350 214 3 11 356 24 362 9.04e-67 214
Msa0169730 MtrunA17_Chr1g0164831 34.302 344 217 6 15 356 2 338 2.22e-66 213
Msa0169730 MtrunA17_Chr2g0278561 34.412 340 212 4 10 343 9 343 1.93e-63 207
Msa0169730 MtrunA17_Chr2g0278551 35.277 343 212 7 11 346 7 346 2.26e-63 207
Msa0169730 MtrunA17_Chr8g0334771 37.764 331 202 4 17 344 11 340 1.90e-62 204
Msa0169730 MtrunA17_Chr2g0332001 35.061 328 207 3 7 331 29 353 4.21e-62 203
Msa0169730 MtrunA17_Chr2g0284261 33.540 322 212 2 13 334 5 324 4.69e-62 202
Msa0169730 MtrunA17_Chr4g0050891 33.639 327 202 5 13 329 10 331 1.38e-61 201
Msa0169730 MtrunA17_Chr3g0126101 35.312 320 204 3 17 336 7 323 1.61e-61 200
Msa0169730 MtrunA17_Chr4g0059311 34.251 327 207 5 15 338 13 334 3.05e-61 200
Msa0169730 MtrunA17_Chr2g0310441 34.431 334 215 3 15 346 9 340 3.75e-61 200
Msa0169730 MtrunA17_Chr8g0383731 35.455 330 205 3 13 335 2 330 1.97e-59 195
Msa0169730 MtrunA17_Chr1g0177221 36.000 250 160 0 13 262 15 264 5.05e-59 191
Msa0169730 MtrunA17_Chr7g0271561 33.333 330 209 4 17 338 8 334 9.12e-58 192
Msa0169730 MtrunA17_Chr3g0129571 34.756 328 206 4 14 334 5 331 1.48e-57 191
Msa0169730 MtrunA17_Chr4g0073241 34.188 351 212 7 17 355 9 352 1.98e-57 191
Msa0169730 MtrunA17_Chr4g0050881 34.808 339 203 8 13 340 6 337 9.97e-57 188
Msa0169730 MtrunA17_Chr4g0050941 34.943 352 206 8 13 346 9 355 2.65e-56 188
Msa0169730 MtrunA17_Chr8g0334751 34.758 351 210 6 15 355 10 351 9.86e-56 186
Msa0169730 MtrunA17_Chr3g0129591 32.081 346 227 4 14 352 3 347 4.14e-54 181
Msa0169730 MtrunA17_Chr5g0413961 33.025 324 210 4 15 338 16 332 1.12e-53 180
Msa0169730 MtrunA17_Chr1g0160111 29.345 351 223 5 25 354 16 362 4.83e-53 179
Msa0169730 MtrunA17_Chr4g0051021 32.615 325 213 4 16 336 14 336 6.78e-53 179
Msa0169730 MtrunA17_Chr5g0408001 31.765 340 214 5 13 336 7 344 1.57e-47 165
Msa0169730 MtrunA17_Chr5g0407991 31.858 339 211 5 15 336 8 343 3.75e-47 164
Msa0169730 MtrunA17_Chr5g0407971 31.965 341 212 6 13 336 8 345 5.36e-47 164
Msa0169730 MtrunA17_Chr1g0150151 33.828 337 210 7 10 338 5 336 5.79e-47 163
Msa0169730 MtrunA17_Chr4g0050931 31.471 340 181 7 13 343 9 305 7.13e-47 161
Msa0169730 MtrunA17_Chr5g0408031 30.836 347 220 5 13 341 7 351 2.93e-46 162
Msa0169730 MtrunA17_Chr3g0126091 31.098 328 220 4 14 336 9 335 7.61e-46 160
Msa0169730 MtrunA17_Chr6g0451171 32.951 349 224 6 12 356 8 350 1.52e-45 159
Msa0169730 MtrunA17_Chr3g0129581 31.792 346 228 4 14 352 3 347 1.17e-44 157
Msa0169730 MtrunA17_Chr5g0408041 30.321 343 219 6 16 340 11 351 1.37e-44 157
Msa0169730 MtrunA17_Chr5g0428881 27.976 336 237 3 3 334 5 339 3.68e-44 156
Msa0169730 MtrunA17_Chr5g0413941 30.719 306 202 5 36 336 27 327 9.52e-44 154
Msa0169730 MtrunA17_Chr5g0413921 29.524 315 206 6 36 342 27 333 1.44e-42 151
Msa0169730 MtrunA17_Chr5g0413931 31.935 310 203 5 36 339 26 333 2.73e-42 150
Msa0169730 MtrunA17_Chr7g0227921 30.000 330 228 3 10 338 5 332 6.95e-42 149
Msa0169730 MtrunA17_Chr6g0451211 32.102 352 226 8 12 356 8 353 7.10e-42 149
Msa0169730 MtrunA17_Chr5g0441761 26.994 326 233 2 21 346 19 339 2.36e-41 148
Msa0169730 MtrunA17_Chr4g0051001 30.820 305 204 3 13 312 28 330 3.28e-40 144
Msa0169730 MtrunA17_Chr5g0404811 37.674 215 128 3 126 336 3 215 1.08e-39 140
Msa0169730 MtrunA17_Chr5g0413951 29.193 322 216 6 21 336 12 327 1.50e-39 143
Msa0169730 MtrunA17_Chr6g0451201 31.124 347 229 7 12 355 8 347 2.20e-38 140
Msa0169730 MtrunA17_Chr8g0369841 28.909 339 223 5 15 337 8 344 4.15e-38 140
Msa0169730 MtrunA17_Chr3g0084091 29.545 264 165 3 91 343 5 258 2.78e-36 133
Msa0169730 MtrunA17_Chr6g0451251 33.667 300 187 8 41 335 37 329 1.28e-35 133
Msa0169730 MtrunA17_Chr6g0451261 30.503 318 213 7 12 325 7 320 1.23e-34 135
Msa0169730 MtrunA17_Chr1g0211801 30.233 258 171 4 9 262 4 256 2.51e-34 127
Msa0169730 MtrunA17_Chr5g0429001 27.839 273 190 2 68 334 3 274 1.92e-31 120
Msa0169730 MtrunA17_Chr6g0451191 34.199 231 142 5 130 356 1 225 7.36e-31 117
Msa0169730 MtrunA17_Chr5g0399281 29.129 333 209 8 26 340 14 337 5.16e-30 117
Msa0169730 MtrunA17_Chr2g0278021 33.191 235 145 5 130 355 1 232 8.19e-29 112
Msa0169730 MtrunA17_Chr4g0056081 27.187 320 214 7 42 349 40 352 2.15e-28 113
Msa0169730 MtrunA17_Chr1g0172881 36.552 145 79 4 122 261 12 148 2.85e-25 99.8
Msa0169730 MtrunA17_Chr8g0334721 43.697 119 65 2 225 343 1 117 8.54e-25 99.0
Msa0169730 MtrunA17_Chr8g0334711 33.673 196 127 3 15 208 9 203 2.60e-23 96.3
Msa0169730 MtrunA17_Chr1g0182261 35.443 158 94 3 200 355 74 225 5.75e-23 95.9
Msa0169730 MtrunA17_Chr1g0211811 47.368 76 38 1 264 337 1 76 2.01e-18 79.3
Msa0169730 MtrunA17_Chr5g0404801 34.454 119 78 0 11 129 2 120 2.98e-17 77.0
Msa0169730 MtrunA17_Chr4g0050921 37.864 103 63 1 213 315 3 104 6.33e-17 76.6
Msa0169730 MtrunA17_Chr2g0278031 34.483 116 76 0 13 128 11 126 1.39e-16 75.5
Msa0169730 MtrunA17_Chr8g0334731 35.135 148 92 3 65 208 40 187 1.75e-16 77.0
Msa0169730 MtrunA17_Chr1g0177231 46.753 77 41 0 260 336 26 102 2.95e-15 71.6
Msa0169730 MtrunA17_Chr1g0172891 42.857 77 44 0 272 348 16 92 2.23e-14 68.9
Msa0169730 MtrunA17_Chr4g0050911 28.244 131 80 3 92 210 7 135 6.86e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0169730 AT5G07050.1 41.690 355 187 5 16 352 17 369 1.16e-89 275
Msa0169730 AT2G39510.1 41.667 324 188 1 13 335 4 327 4.81e-87 267
Msa0169730 AT2G37460.1 40.062 322 192 1 13 334 11 331 1.56e-86 266
Msa0169730 AT4G08300.1 38.757 338 202 3 13 346 7 343 1.55e-82 256
Msa0169730 AT1G44800.1 40.237 338 196 4 13 346 7 342 2.11e-81 253
Msa0169730 AT2G40900.1 37.805 328 199 2 9 336 3 325 7.11e-80 249
Msa0169730 AT1G75500.1 36.695 357 211 3 11 356 14 366 1.88e-78 246
Msa0169730 AT1G75500.2 36.695 357 211 3 11 356 14 366 1.88e-78 246
Msa0169730 AT3G18200.2 38.235 340 206 3 2 340 20 356 1.24e-76 241
Msa0169730 AT5G13670.1 37.048 332 196 4 12 335 3 329 4.15e-76 239
Msa0169730 AT1G21890.1 36.119 335 203 2 13 337 7 340 1.85e-75 238
Msa0169730 AT4G08290.1 37.576 330 198 4 14 338 10 336 5.24e-75 237
Msa0169730 AT3G56620.1 38.650 326 194 3 9 333 3 323 1.68e-74 235
Msa0169730 AT2G37450.2 37.654 324 180 2 13 336 5 306 3.85e-70 223
Msa0169730 AT1G43650.1 36.036 333 210 2 14 346 4 333 3.28e-69 221
Msa0169730 AT4G30420.2 35.278 360 211 5 13 356 5 358 7.98e-68 218
Msa0169730 AT4G30420.1 35.028 354 208 5 19 356 1 348 5.20e-65 211
Msa0169730 AT1G21890.2 35.161 310 190 2 13 312 7 315 6.87e-65 209
Msa0169730 AT3G30340.1 33.815 346 222 3 13 356 7 347 3.47e-64 208
Msa0169730 AT5G64700.1 34.211 342 216 3 16 349 7 347 8.56e-64 207
Msa0169730 AT3G53210.1 33.523 352 214 6 16 355 9 352 6.85e-63 205
Msa0169730 AT1G09380.1 32.710 321 210 2 17 332 8 327 1.80e-60 199
Msa0169730 AT2G37450.3 40.426 235 139 1 102 336 1 234 4.89e-55 181
Msa0169730 AT3G18200.1 40.870 230 130 4 113 340 1 226 4.36e-53 176
Msa0169730 AT4G01440.1 29.154 319 224 2 14 331 7 324 1.21e-51 176
Msa0169730 AT1G11450.3 30.357 336 232 2 11 345 10 344 1.43e-48 167
Msa0169730 AT5G40210.1 30.218 321 206 5 36 355 32 335 4.57e-48 166
Msa0169730 AT1G68170.2 30.422 332 224 4 10 336 6 335 1.07e-47 165
Msa0169730 AT1G01070.1 29.630 351 233 6 6 349 5 348 1.51e-47 165
Msa0169730 AT4G08290.2 34.783 253 157 4 14 261 10 259 1.84e-46 160
Msa0169730 AT1G68170.1 30.841 321 215 4 21 336 7 325 6.85e-46 160
Msa0169730 AT3G28050.1 30.904 343 220 9 19 355 17 348 2.00e-45 159
Msa0169730 AT1G11450.5 32.095 296 199 2 51 345 6 300 2.82e-45 157
Msa0169730 AT2G37450.1 39.904 208 124 1 129 336 61 267 9.07e-45 155
Msa0169730 AT4G01430.1 28.571 350 241 7 13 355 5 352 1.02e-44 157
Msa0169730 AT4G01440.3 27.667 300 215 2 14 312 7 305 1.88e-44 155
Msa0169730 AT3G53210.2 32.491 277 167 6 91 355 27 295 3.18e-44 155
Msa0169730 AT1G70260.1 27.119 354 226 4 13 346 8 349 8.11e-44 155
Msa0169730 AT1G11450.2 31.849 292 197 2 55 345 3 293 1.06e-43 153
Msa0169730 AT1G11460.1 29.394 330 230 3 9 336 8 336 3.37e-43 153
Msa0169730 AT4G28040.1 27.168 346 236 5 13 353 4 338 3.96e-43 153
Msa0169730 AT4G28040.5 27.168 346 236 5 13 353 4 338 3.96e-43 153
Msa0169730 AT4G28040.3 27.168 346 236 5 13 353 4 338 3.96e-43 153
Msa0169730 AT4G28040.4 27.168 346 236 5 13 353 4 338 3.96e-43 153
Msa0169730 AT4G28040.2 27.168 346 236 5 13 353 4 338 3.96e-43 153
Msa0169730 AT1G25270.1 28.986 345 238 4 15 354 1 343 1.08e-42 152
Msa0169730 AT1G43650.2 33.871 248 161 2 14 261 4 248 1.11e-42 149
Msa0169730 AT5G64700.2 33.071 254 161 3 16 261 7 259 1.96e-41 146
Msa0169730 AT1G60050.1 27.557 352 237 6 17 355 13 359 1.01e-40 147
Msa0169730 AT5G40230.1 31.579 304 199 6 41 338 45 345 1.16e-40 147
Msa0169730 AT3G28130.2 29.755 326 217 6 21 339 17 337 2.14e-40 145
Msa0169730 AT3G53210.3 32.411 253 153 4 16 263 9 248 7.09e-40 142
Msa0169730 AT5G40240.1 29.605 304 205 5 41 338 44 344 6.06e-39 142
Msa0169730 AT5G40240.3 29.605 304 205 5 41 338 44 344 6.06e-39 142
Msa0169730 AT5G40240.2 29.605 304 205 5 41 338 58 358 7.52e-39 142
Msa0169730 AT5G45370.2 28.358 335 222 5 18 336 21 353 1.62e-38 141
Msa0169730 AT5G45370.4 28.358 335 222 5 18 336 21 353 1.62e-38 141
Msa0169730 AT5G45370.1 27.566 341 226 5 18 354 21 344 1.79e-38 140
Msa0169730 AT3G28130.5 32.245 245 160 3 99 339 9 251 1.25e-37 136
Msa0169730 AT3G28130.1 32.245 245 160 3 99 339 9 251 1.25e-37 136
Msa0169730 AT1G01070.2 30.952 294 189 6 63 349 15 301 2.68e-37 137
Msa0169730 AT1G11450.4 28.713 303 214 2 11 312 10 311 2.92e-37 137
Msa0169730 AT3G28100.1 29.114 316 213 7 26 336 26 335 1.49e-36 135
Msa0169730 AT4G24980.1 28.146 302 151 6 34 335 4 239 4.86e-36 132
Msa0169730 AT4G01440.5 30.841 214 146 2 119 331 4 216 9.56e-36 131
Msa0169730 AT4G01440.2 30.841 214 146 2 119 331 4 216 9.56e-36 131
Msa0169730 AT3G28070.2 30.677 251 168 4 99 344 7 256 4.87e-35 129
Msa0169730 AT4G15540.1 28.824 340 224 6 1 336 1 326 5.70e-35 131
Msa0169730 AT4G15540.2 28.824 340 224 6 1 336 1 326 5.70e-35 131
Msa0169730 AT3G30340.2 30.556 252 172 2 13 262 7 257 6.82e-35 129
Msa0169730 AT3G28070.1 30.677 251 168 4 99 344 99 348 4.77e-34 129
Msa0169730 AT3G28080.1 28.819 288 194 7 65 344 62 346 9.43e-34 128
Msa0169730 AT3G28080.5 29.200 250 168 5 102 344 10 257 9.45e-34 126
Msa0169730 AT3G28080.7 29.200 250 168 5 102 344 10 257 9.45e-34 126
Msa0169730 AT3G28080.8 29.200 250 168 5 102 344 10 257 9.45e-34 126
Msa0169730 AT3G28080.6 29.200 250 168 5 102 344 10 257 9.45e-34 126
Msa0169730 AT4G01430.2 26.885 305 217 5 13 312 5 308 1.63e-33 126
Msa0169730 AT1G11450.1 31.325 249 169 2 98 345 5 252 2.90e-33 124
Msa0169730 AT3G28080.2 29.200 250 168 5 102 344 91 338 6.14e-33 125
Msa0169730 AT3G45870.1 28.916 332 216 6 13 329 8 334 7.75e-32 123
Msa0169730 AT4G01450.2 27.864 323 221 3 14 332 7 321 1.58e-31 122
Msa0169730 AT4G19185.1 27.515 338 221 6 18 336 22 354 1.61e-31 123
Msa0169730 AT4G01440.4 27.344 256 172 4 14 262 7 255 1.00e-30 118
Msa0169730 AT4G01450.1 26.733 303 210 3 14 312 7 301 1.09e-30 119
Msa0169730 AT5G47470.3 27.723 303 206 5 42 338 83 378 1.17e-29 117
Msa0169730 AT5G47470.2 27.723 303 206 5 42 338 83 378 1.41e-29 117
Msa0169730 AT5G47470.1 27.723 303 206 5 42 338 55 350 1.42e-29 117
Msa0169730 AT3G28130.3 31.776 214 140 3 130 339 1 212 2.41e-29 113
Msa0169730 AT3G28070.5 30.455 220 147 4 130 344 1 219 5.87e-27 107
Msa0169730 AT3G45870.8 27.937 315 207 6 13 312 8 317 2.67e-26 107
Msa0169730 AT3G28070.3 30.841 214 144 3 102 312 102 314 3.93e-26 106
Msa0169730 AT3G28080.9 28.378 222 150 5 130 344 1 220 1.19e-25 103
Msa0169730 AT4G01450.4 27.801 241 162 3 14 250 7 239 1.00e-24 101
Msa0169730 AT5G45370.6 27.888 251 163 5 102 336 3 251 8.05e-24 99.8
Msa0169730 AT5G45370.5 27.888 251 163 5 102 336 3 251 8.05e-24 99.8
Msa0169730 AT4G19185.2 27.413 259 164 6 97 336 23 276 1.07e-23 100
Msa0169730 AT4G16620.1 25.915 328 220 6 24 338 19 336 2.97e-23 99.8
Msa0169730 AT4G01450.3 27.928 222 149 2 14 231 7 221 5.89e-23 96.7
Msa0169730 AT4G01430.3 29.954 217 144 6 145 355 1 215 7.77e-22 93.2
Msa0169730 AT3G28100.3 29.583 240 159 6 26 260 26 260 1.25e-20 90.5
Msa0169730 AT3G28130.4 28.112 249 168 5 21 262 17 261 4.41e-20 89.0
Msa0169730 AT3G45870.9 28.821 229 151 3 13 229 8 236 9.95e-18 82.8
Msa0169730 AT3G45870.5 29.279 222 137 6 123 329 43 259 2.02e-17 82.4
Msa0169730 AT5G45370.3 26.577 222 145 5 131 336 74 293 8.42e-17 80.5
Msa0169730 AT4G16620.4 26.877 253 155 6 102 338 1 239 4.89e-16 77.4
Msa0169730 AT4G16620.2 26.877 253 155 6 102 338 1 239 4.89e-16 77.4
Msa0169730 AT3G45870.2 30.769 143 91 3 190 329 46 183 1.72e-14 72.4
Msa0169730 AT3G28060.1 32.432 111 74 1 202 312 45 154 1.68e-13 67.8
Msa0169730 AT3G28100.2 32.374 139 89 3 26 163 26 160 1.16e-12 67.8
Msa0169730 AT3G45870.6 27.805 205 128 6 123 312 43 242 2.30e-12 66.6
Msa0169730 AT3G45870.7 27.805 205 128 6 123 312 43 242 2.30e-12 66.6
Msa0169730 AT3G28130.6 28.767 146 98 3 21 163 17 159 1.18e-11 63.9

Find 91 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAAGTGTCCTATAATT+AGG 0.139339 1_4:-53014742 None:intergenic
ATGTAACTGATTTGTCTAAA+TGG 0.187646 1_4:-53014619 None:intergenic
ACTACATGGATGTGCTTTAT+TGG 0.204661 1_4:+53013781 Msa0169730:CDS
AAACCAACCCTCTCTCTTAA+AGG 0.205488 1_4:-53014839 None:intergenic
GACTTGTGGTCCATAATATA+TGG 0.217089 1_4:+53013880 Msa0169730:CDS
ATATTTGTGCTTTCTTTGTT+AGG 0.235092 1_4:+53012895 Msa0169730:CDS
TATTGATCTACATTCAATTA+TGG 0.238153 1_4:+53014022 Msa0169730:CDS
GAGGATTTGTAGGTTTATTC+TGG 0.257153 1_4:+53014505 Msa0169730:three_prime_UTR
TATCAACGAGAGTGGATTAA+AGG 0.268996 1_4:+53013575 Msa0169730:CDS
GCTATATTCAGCATTCTTCT+AGG 0.280481 1_4:-53014426 None:intergenic
TCAAAAGTGTCCTATAATTA+GGG 0.284980 1_4:-53014741 None:intergenic
AAACCTAACCCCATATATTA+TGG 0.288350 1_4:-53013890 None:intergenic
AACCCTTCAGCATGGATTAT+AGG 0.289438 1_4:+53013847 Msa0169730:CDS
CTACCTCCGTCCCTAATTAT+AGG 0.295420 1_4:+53014731 Msa0169730:three_prime_UTR
TTCAGAGATAAAATATAAAA+AGG 0.306735 1_4:-53012353 None:intergenic
ATTTCAAGACCAAAGTTAAA+AGG 0.310975 1_4:+53014383 Msa0169730:CDS
ACCCTTCAGCATGGATTATA+GGG 0.326309 1_4:+53013848 Msa0169730:CDS
CCCTCTTTGTACTATATTTG+TGG 0.328346 1_4:+53014086 Msa0169730:CDS
AATATGTTTGTAATTACTAT+TGG 0.348055 1_4:+53014802 Msa0169730:three_prime_UTR
ATGTAATTCTTTAGCATCAT+AGG 0.352746 1_4:+53014295 Msa0169730:intron
AAACATCATCACTAAACTTA+TGG 0.354829 1_4:+53012249 Msa0169730:exon
TGCCACAAGTTTCTCATAAC+AGG 0.377701 1_4:-53013516 None:intergenic
TGCACATCATGAGGATTTGT+AGG 0.395629 1_4:+53014495 Msa0169730:three_prime_UTR
ACTTGTGGTCCATAATATAT+GGG 0.400807 1_4:+53013881 Msa0169730:CDS
GGTTGAATAATATGGATTAG+AGG 0.401678 1_4:-53013540 None:intergenic
TATTAATGACAGGGAATTGT+TGG 0.403386 1_4:+53013991 Msa0169730:intron
AGGTGCATTTATTGTCATCA+TGG 0.421517 1_4:+53014315 Msa0169730:CDS
GGGTTTGTATCTGTTGCTGT+GGG 0.425250 1_4:+53014336 Msa0169730:CDS
TATTTGTGCTTTCTTTGTTA+GGG 0.426849 1_4:+53012896 Msa0169730:CDS
ACCTATCGGCATGTTCTCGC+CGG 0.429528 1_4:+53012415 Msa0169730:CDS
CCTATCGGCATGTTCTCGCC+GGG 0.430648 1_4:+53012416 Msa0169730:CDS
TTCTATATGGTACATTATGC+AGG 0.432883 1_4:+53013632 Msa0169730:CDS
CTCAATTATCACTCACTACA+TGG 0.439857 1_4:+53013767 Msa0169730:CDS
ATCAACGAGAGTGGATTAAA+GGG 0.446252 1_4:+53013576 Msa0169730:CDS
TGTAACTGATTTGTCTAAAT+GGG 0.458412 1_4:-53014618 None:intergenic
AAAAGAACAAGTGTCAGCTT+TGG 0.474816 1_4:-53012865 None:intergenic
TCTGAATCTTCCTTCAATCA+TGG 0.477623 1_4:+53012370 Msa0169730:CDS
TGGGTTTGTATCTGTTGCTG+TGG 0.482889 1_4:+53014335 Msa0169730:CDS
GTTCAAGCCACACCTTATCA+TGG 0.485715 1_4:+53012309 Msa0169730:CDS
GGGACCTTGATATCCTTAGC+TGG 0.486715 1_4:+53013465 Msa0169730:CDS
CTCTCAAGAAAGTATGCAAA+AGG 0.494726 1_4:-53012451 None:intergenic
AACACCAGCTAAGGATATCA+AGG 0.497528 1_4:-53013469 None:intergenic
GTTTGTATCTGTTGCTGTGG+GGG 0.500052 1_4:+53014338 Msa0169730:CDS
AACATGGAAGCAAGAAATGT+TGG 0.500297 1_4:-53013048 None:intergenic
GGTGCATTTATTGTCATCAT+GGG 0.503602 1_4:+53014316 Msa0169730:CDS
GTAGTGAGTGATAATTGAGC+AGG 0.509226 1_4:-53013763 None:intergenic
CTTATGTTTATGTTACCTAT+CGG 0.512879 1_4:+53012401 Msa0169730:CDS
TGACAGGGAATTGTTGGTGG+TGG 0.516639 1_4:+53013997 Msa0169730:intron
ATACTAGCATACTTCGTCCT+TGG 0.517108 1_4:+53014111 Msa0169730:CDS
TTGACTTGAATGCAGGATTG+AGG 0.518118 1_4:+53013401 Msa0169730:intron
AATAAAATTTGCACATCATG+AGG 0.520081 1_4:+53014486 Msa0169730:three_prime_UTR
TTTCACCATGACACTATACA+AGG 0.524518 1_4:+53013491 Msa0169730:CDS
TAGCTAGAAATTCGCATCGA+TGG 0.525618 1_4:-53014701 None:intergenic
ATTTAGACAAATCAGTTACA+TGG 0.531067 1_4:+53014621 Msa0169730:three_prime_UTR
TCATTATTGCAGTGGCACTC+AGG 0.535841 1_4:+53013094 Msa0169730:CDS
CTTGGGCTAAAACCATGATA+AGG 0.551206 1_4:-53012321 None:intergenic
CACTGCAATAATGAAGGTAA+TGG 0.555306 1_4:-53013085 None:intergenic
AACTCTTTGTATACATGTAG+AGG 0.556101 1_4:-53012289 None:intergenic
CATTACCTTCATTATTGCAG+TGG 0.559816 1_4:+53013086 Msa0169730:CDS
AGTTCTTCTATCAACGAGAG+TGG 0.559959 1_4:+53013567 Msa0169730:CDS
CGATAGGTAACATAAACATA+AGG 0.561067 1_4:-53012400 None:intergenic
TTATATATATAGTGGGAATG+TGG 0.561311 1_4:+53012200 None:intergenic
AGCCCTATAATCCATGCTGA+AGG 0.566395 1_4:-53013850 None:intergenic
CATGGTGAAAACACCAGCTA+AGG 0.566514 1_4:-53013478 None:intergenic
GAGTGCCACTGCAATAATGA+AGG 0.571106 1_4:-53013091 None:intergenic
AGGACTCATTCCATGATTGA+AGG 0.576140 1_4:-53012380 None:intergenic
GGTTTGTATCTGTTGCTGTG+GGG 0.597926 1_4:+53014337 Msa0169730:CDS
TAATGACAGGGAATTGTTGG+TGG 0.600813 1_4:+53013994 Msa0169730:intron
GCCACAAATATAGTACAAAG+AGG 0.605176 1_4:-53014087 None:intergenic
GCCCTATAATCCATGCTGAA+GGG 0.606291 1_4:-53013849 None:intergenic
AGGTCCCTTGTATAGTGTCA+TGG 0.606603 1_4:-53013496 None:intergenic
AATAACGGGAATTAAGATGG+TGG 0.609943 1_4:+53014767 Msa0169730:three_prime_UTR
CCACAAATATAGTACAAAGA+GGG 0.611746 1_4:-53014086 None:intergenic
AACATAATAACCCTTCAGCA+TGG 0.615122 1_4:+53013839 Msa0169730:CDS
CTTGTGGTCCATAATATATG+GGG 0.619780 1_4:+53013882 Msa0169730:CDS
AAGTGTCCTATAATTAGGGA+CGG 0.625666 1_4:-53014737 None:intergenic
GGAAGCTATAGTGTTGAACA+TGG 0.625781 1_4:-53013064 None:intergenic
ATAGCATGTAACTTTAGTAG+AGG 0.631961 1_4:+53014444 Msa0169730:three_prime_UTR
TAGGGCTCAACATTGACTTG+TGG 0.638445 1_4:+53013866 Msa0169730:CDS
CCCGGCGAGAACATGCCGAT+AGG 0.651355 1_4:-53012416 None:intergenic
TAGTGAGTGATAATTGAGCA+GGG 0.654187 1_4:-53013762 None:intergenic
AAAATATGGCAGGATCACCA+TGG 0.660749 1_4:+53012987 Msa0169730:intron
CTATCGGCATGTTCTCGCCG+GGG 0.671878 1_4:+53012417 Msa0169730:CDS
GCTAAAACCATGATAAGGTG+TGG 0.672505 1_4:-53012316 None:intergenic
GGGGAAAGAAGGTGACAAAG+AGG 0.675637 1_4:+53014357 Msa0169730:CDS
AAAAGGAAACATCACAACCC+CGG 0.676563 1_4:-53012434 None:intergenic
TTCACCATGACACTATACAA+GGG 0.685500 1_4:+53013492 Msa0169730:CDS
AAAGTACAAATTGAGTGCCA+TGG 0.689345 1_4:-53013004 None:intergenic
TGTCCTATAATTAGGGACGG+AGG 0.704051 1_4:-53014734 None:intergenic
AATGTACCATATAGAAAACG+TGG 0.726303 1_4:-53013625 None:intergenic
GACCTGTTATGAGAAACTTG+TGG 0.744585 1_4:+53013514 Msa0169730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAAAATAGAAATTTTATA+AGG - chr1_4:53013164-53013183 None:intergenic 10.0%
!!! AAATTTCTATTTTTTGTTTT+TGG + chr1_4:53013168-53013187 Msa0169730:intron 10.0%
!!! AAAATTTAATCTATTGATTT+CGG + chr1_4:53013339-53013358 Msa0169730:intron 10.0%
!! TAATGTTAGAAATTTATATT+TGG - chr1_4:53013955-53013974 None:intergenic 10.0%
!! TATTCATAGTAAAAAAAAAT+TGG - chr1_4:53014200-53014219 None:intergenic 10.0%
!! TTCAGAGATAAAATATAAAA+AGG - chr1_4:53012356-53012375 None:intergenic 15.0%
!!! ATGTTTTTAGTTTTGTAATT+TGG - chr1_4:53013219-53013238 None:intergenic 15.0%
!!! ATAATTGTAGTTCTAAAATT+TGG + chr1_4:53014269-53014288 Msa0169730:intron 15.0%
!!! CAAATTTTATTCTAAATACT+TGG - chr1_4:53014477-53014496 None:intergenic 15.0%
!! AATATGTTTGTAATTACTAT+TGG + chr1_4:53014802-53014821 Msa0169730:three_prime_UTR 15.0%
!! ATGTATGTTATTACATTGTA+TGG + chr1_4:53012935-53012954 Msa0169730:intron 20.0%
!!! GTATAATTTTAAAATATGGC+AGG + chr1_4:53012977-53012996 Msa0169730:intron 20.0%
!!! TTTTAGTTTTGTAATTTGGA+AGG - chr1_4:53013215-53013234 None:intergenic 20.0%
!!! AACTTTTTGGTTGAATAATA+TGG - chr1_4:53013551-53013570 None:intergenic 20.0%
!! TAAAAACTAGTTATGAGTTA+AGG - chr1_4:53013664-53013683 None:intergenic 20.0%
!! AACTCTAATATCTTTGTAAT+TGG + chr1_4:53013711-53013730 Msa0169730:intron 20.0%
!! AATCATTCAATATTAATGAC+AGG + chr1_4:53013981-53014000 Msa0169730:intron 20.0%
!! ATCATTCAATATTAATGACA+GGG + chr1_4:53013982-53014001 Msa0169730:intron 20.0%
!! TATTGATCTACATTCAATTA+TGG + chr1_4:53014022-53014041 Msa0169730:CDS 20.0%
!!! TTTTATTCTAAATACTTGGA+TGG - chr1_4:53014473-53014492 None:intergenic 20.0%
! AAACATCATCACTAAACTTA+TGG + chr1_4:53012249-53012268 Msa0169730:exon 25.0%
! CTTATGTTTATGTTACCTAT+CGG + chr1_4:53012401-53012420 Msa0169730:CDS 25.0%
! ATATTTGTGCTTTCTTTGTT+AGG + chr1_4:53012895-53012914 Msa0169730:CDS 25.0%
! TATTTGTGCTTTCTTTGTTA+GGG + chr1_4:53012896-53012915 Msa0169730:CDS 25.0%
! TATTACATTGTATGGAAGTT+TGG + chr1_4:53012943-53012962 Msa0169730:intron 25.0%
! ATCTTTGTAATTGGAAATTC+AGG + chr1_4:53013720-53013739 Msa0169730:intron 25.0%
! TTAAATACAGTGACAAAAAC+AGG - chr1_4:53014066-53014085 None:intergenic 25.0%
! TACTATGAATATGTAGTAAG+TGG + chr1_4:53014209-53014228 Msa0169730:intron 25.0%
! ATGTAATTCTTTAGCATCAT+AGG + chr1_4:53014295-53014314 Msa0169730:intron 25.0%
! ATTTCAAGACCAAAGTTAAA+AGG + chr1_4:53014383-53014402 Msa0169730:CDS 25.0%
! AATAAAATTTGCACATCATG+AGG + chr1_4:53014486-53014505 Msa0169730:three_prime_UTR 25.0%
! AGACAAAAATTTAGTCTATG+TGG + chr1_4:53014595-53014614 Msa0169730:three_prime_UTR 25.0%
!!! TGTAACTGATTTGTCTAAAT+GGG - chr1_4:53014621-53014640 None:intergenic 25.0%
!!! ATGTAACTGATTTGTCTAAA+TGG - chr1_4:53014622-53014641 None:intergenic 25.0%
! ATTTAGACAAATCAGTTACA+TGG + chr1_4:53014621-53014640 Msa0169730:three_prime_UTR 25.0%
!!! TCAAAAGTGTCCTATAATTA+GGG - chr1_4:53014744-53014763 None:intergenic 25.0%
!!! TTCAAAAGTGTCCTATAATT+AGG - chr1_4:53014745-53014764 None:intergenic 25.0%
!!! GGACACTTTTGAAAAAATAA+CGG + chr1_4:53014752-53014771 Msa0169730:three_prime_UTR 25.0%
!!! GACACTTTTGAAAAAATAAC+GGG + chr1_4:53014753-53014772 Msa0169730:three_prime_UTR 25.0%
!! AAAAATAACGGGAATTAAGA+TGG + chr1_4:53014764-53014783 Msa0169730:three_prime_UTR 25.0%
AACTCTTTGTATACATGTAG+AGG - chr1_4:53012292-53012311 None:intergenic 30.0%
! TAAAAAGGTATAGCCAACTT+GGG - chr1_4:53012341-53012360 None:intergenic 30.0%
! ATAAAAAGGTATAGCCAACT+TGG - chr1_4:53012342-53012361 None:intergenic 30.0%
CGATAGGTAACATAAACATA+AGG - chr1_4:53012403-53012422 None:intergenic 30.0%
! CAAAGTTCATAGTTTGTGTT+TGG + chr1_4:53012503-53012522 Msa0169730:intron 30.0%
!! TTAATCTTCTAGCTTAACCA+AGG - chr1_4:53012690-53012709 None:intergenic 30.0%
GTTCGCAATTCTTGATATAA+TGG + chr1_4:53013116-53013135 Msa0169730:intron 30.0%
GTAGCATAGCAAAAATTCTT+GGG + chr1_4:53013445-53013464 Msa0169730:CDS 30.0%
!! GGTTGAATAATATGGATTAG+AGG - chr1_4:53013543-53013562 None:intergenic 30.0%
!! TCGTTGATAGAAGAACTTTT+TGG - chr1_4:53013564-53013583 None:intergenic 30.0%
AATGTACCATATAGAAAACG+TGG - chr1_4:53013628-53013647 None:intergenic 30.0%
TTCTATATGGTACATTATGC+AGG + chr1_4:53013632-53013651 Msa0169730:CDS 30.0%
ACTTGTGGTCCATAATATAT+GGG + chr1_4:53013881-53013900 Msa0169730:CDS 30.0%
AAACCTAACCCCATATATTA+TGG - chr1_4:53013893-53013912 None:intergenic 30.0%
TATTAATGACAGGGAATTGT+TGG + chr1_4:53013991-53014010 Msa0169730:intron 30.0%
CCACAAATATAGTACAAAGA+GGG - chr1_4:53014089-53014108 None:intergenic 30.0%
CTTGGTGAAAAACTTTATCT+GGG + chr1_4:53014129-53014148 Msa0169730:CDS 30.0%
!!! TTTGTACTGCCTTTTAACTT+TGG - chr1_4:53014395-53014414 None:intergenic 30.0%
GTTAAAAGGCAGTACAAAAA+TGG + chr1_4:53014397-53014416 Msa0169730:CDS 30.0%
ATAGCATGTAACTTTAGTAG+AGG + chr1_4:53014444-53014463 Msa0169730:three_prime_UTR 30.0%
TCTGAATCTTCCTTCAATCA+TGG + chr1_4:53012370-53012389 Msa0169730:CDS 35.0%
! CTCTCAAGAAAGTATGCAAA+AGG - chr1_4:53012454-53012473 None:intergenic 35.0%
! CTTTTGCATACTTTCTTGAG+AGG + chr1_4:53012452-53012471 Msa0169730:CDS 35.0%
! AATCATGATTGTAAGGCACT+CGG - chr1_4:53012529-53012548 None:intergenic 35.0%
TGCCTTACAATCATGATTCT+TGG + chr1_4:53012531-53012550 Msa0169730:intron 35.0%
CACCAAGAATCATGATTGTA+AGG - chr1_4:53012536-53012555 None:intergenic 35.0%
AAAAGAACAAGTGTCAGCTT+TGG - chr1_4:53012868-53012887 None:intergenic 35.0%
! GCTGACACTTGTTCTTTTTA+TGG + chr1_4:53012870-53012889 Msa0169730:CDS 35.0%
AAAGTACAAATTGAGTGCCA+TGG - chr1_4:53013007-53013026 None:intergenic 35.0%
AACATGGAAGCAAGAAATGT+TGG - chr1_4:53013051-53013070 None:intergenic 35.0%
CACTGCAATAATGAAGGTAA+TGG - chr1_4:53013088-53013107 None:intergenic 35.0%
CATTACCTTCATTATTGCAG+TGG + chr1_4:53013086-53013105 Msa0169730:CDS 35.0%
AATTTGGAAGGACACAAAAG+TGG - chr1_4:53013203-53013222 None:intergenic 35.0%
CTAGATTCTTCAACGGATAT+TGG + chr1_4:53013275-53013294 Msa0169730:intron 35.0%
!! AGAATTTTTGCTATGCTACG+TGG - chr1_4:53013444-53013463 None:intergenic 35.0%
CGTAGCATAGCAAAAATTCT+TGG + chr1_4:53013444-53013463 Msa0169730:CDS 35.0%
TTTCACCATGACACTATACA+AGG + chr1_4:53013491-53013510 Msa0169730:CDS 35.0%
TTCACCATGACACTATACAA+GGG + chr1_4:53013492-53013511 Msa0169730:CDS 35.0%
!! TATCAACGAGAGTGGATTAA+AGG + chr1_4:53013575-53013594 Msa0169730:CDS 35.0%
!! ATCAACGAGAGTGGATTAAA+GGG + chr1_4:53013576-53013595 Msa0169730:CDS 35.0%
TAGTGAGTGATAATTGAGCA+GGG - chr1_4:53013765-53013784 None:intergenic 35.0%
CTCAATTATCACTCACTACA+TGG + chr1_4:53013767-53013786 Msa0169730:CDS 35.0%
ACTACATGGATGTGCTTTAT+TGG + chr1_4:53013781-53013800 Msa0169730:CDS 35.0%
AACATAATAACCCTTCAGCA+TGG + chr1_4:53013839-53013858 Msa0169730:CDS 35.0%
GACTTGTGGTCCATAATATA+TGG + chr1_4:53013880-53013899 Msa0169730:CDS 35.0%
CTTGTGGTCCATAATATATG+GGG + chr1_4:53013882-53013901 Msa0169730:CDS 35.0%
TTATGGTGCACTGAGAAAAA+AGG + chr1_4:53014039-53014058 Msa0169730:CDS 35.0%
TATGGTGCACTGAGAAAAAA+GGG + chr1_4:53014040-53014059 Msa0169730:CDS 35.0%
CCCTCTTTGTACTATATTTG+TGG + chr1_4:53014086-53014105 Msa0169730:CDS 35.0%
GCCACAAATATAGTACAAAG+AGG - chr1_4:53014090-53014109 None:intergenic 35.0%
! CCAGATAAAGTTTTTCACCA+AGG - chr1_4:53014131-53014150 None:intergenic 35.0%
CCTTGGTGAAAAACTTTATC+TGG + chr1_4:53014128-53014147 Msa0169730:CDS 35.0%
! TGGGCAGGTAAAACTTTTAA+CGG + chr1_4:53014148-53014167 Msa0169730:intron 35.0%
AGGTGCATTTATTGTCATCA+TGG + chr1_4:53014315-53014334 Msa0169730:CDS 35.0%
GGTGCATTTATTGTCATCAT+GGG + chr1_4:53014316-53014335 Msa0169730:CDS 35.0%
AAAAGGCAGTACAAAAATGG+AGG + chr1_4:53014400-53014419 Msa0169730:CDS 35.0%
GCTATATTCAGCATTCTTCT+AGG - chr1_4:53014429-53014448 None:intergenic 35.0%
GAGGATTTGTAGGTTTATTC+TGG + chr1_4:53014505-53014524 Msa0169730:three_prime_UTR 35.0%
AAGTGTCCTATAATTAGGGA+CGG - chr1_4:53014740-53014759 None:intergenic 35.0%
! AATAACGGGAATTAAGATGG+TGG + chr1_4:53014767-53014786 Msa0169730:three_prime_UTR 35.0%
GCTAAAACCATGATAAGGTG+TGG - chr1_4:53012319-53012338 None:intergenic 40.0%
CTTGGGCTAAAACCATGATA+AGG - chr1_4:53012324-53012343 None:intergenic 40.0%
!!! ATCATGGTTTTAGCCCAAGT+TGG + chr1_4:53012325-53012344 Msa0169730:CDS 40.0%
AGGACTCATTCCATGATTGA+AGG - chr1_4:53012383-53012402 None:intergenic 40.0%
AAAAGGAAACATCACAACCC+CGG - chr1_4:53012437-53012456 None:intergenic 40.0%
TCTTGTGAGATCCAAATGGA+CGG + chr1_4:53012604-53012623 Msa0169730:intron 40.0%
CAGCAGCATTAAGACTCTTA+GGG - chr1_4:53012638-53012657 None:intergenic 40.0%
! TCTAGCTTAACCAAGGTTCT+GGG - chr1_4:53012683-53012702 None:intergenic 40.0%
!! TTCTAGCTTAACCAAGGTTC+TGG - chr1_4:53012684-53012703 None:intergenic 40.0%
AAAATATGGCAGGATCACCA+TGG + chr1_4:53012987-53013006 Msa0169730:intron 40.0%
! GGAAGCTATAGTGTTGAACA+TGG - chr1_4:53013067-53013086 None:intergenic 40.0%
GTGAGTGCTAGATTCTTCAA+CGG + chr1_4:53013268-53013287 Msa0169730:intron 40.0%
!! AAGAGAGTTGACTTGAATGC+AGG + chr1_4:53013394-53013413 Msa0169730:intron 40.0%
! TTGACTTGAATGCAGGATTG+AGG + chr1_4:53013401-53013420 Msa0169730:intron 40.0%
AACACCAGCTAAGGATATCA+AGG - chr1_4:53013472-53013491 None:intergenic 40.0%
GACCTGTTATGAGAAACTTG+TGG + chr1_4:53013514-53013533 Msa0169730:CDS 40.0%
TGCCACAAGTTTCTCATAAC+AGG - chr1_4:53013519-53013538 None:intergenic 40.0%
AGTTCTTCTATCAACGAGAG+TGG + chr1_4:53013567-53013586 Msa0169730:CDS 40.0%
! GTTGTGCCACGTTTTCTATA+TGG + chr1_4:53013619-53013638 Msa0169730:CDS 40.0%
GTAGTGAGTGATAATTGAGC+AGG - chr1_4:53013766-53013785 None:intergenic 40.0%
! AACCCTTCAGCATGGATTAT+AGG + chr1_4:53013847-53013866 Msa0169730:CDS 40.0%
! ACCCTTCAGCATGGATTATA+GGG + chr1_4:53013848-53013867 Msa0169730:CDS 40.0%
GGTCCATAATATATGGGGTT+AGG + chr1_4:53013887-53013906 Msa0169730:intron 40.0%
TAATGACAGGGAATTGTTGG+TGG + chr1_4:53013994-53014013 Msa0169730:intron 40.0%
ATACTAGCATACTTCGTCCT+TGG + chr1_4:53014111-53014130 Msa0169730:CDS 40.0%
GTGAAAAACTTTATCTGGGC+AGG + chr1_4:53014133-53014152 Msa0169730:CDS 40.0%
TGCACATCATGAGGATTTGT+AGG + chr1_4:53014495-53014514 Msa0169730:three_prime_UTR 40.0%
TAGCTAGAAATTCGCATCGA+TGG - chr1_4:53014704-53014723 None:intergenic 40.0%
GTTCAAGCCACACCTTATCA+TGG + chr1_4:53012309-53012328 Msa0169730:CDS 45.0%
TTGGATCTCACAAGACTCGA+GGG - chr1_4:53012599-53012618 None:intergenic 45.0%
TTTGGATCTCACAAGACTCG+AGG - chr1_4:53012600-53012619 None:intergenic 45.0%
! CGAGTCTTGTGAGATCCAAA+TGG + chr1_4:53012600-53012619 Msa0169730:intron 45.0%
GCAGCAGCATTAAGACTCTT+AGG - chr1_4:53012639-53012658 None:intergenic 45.0%
! GAGTGCCACTGCAATAATGA+AGG - chr1_4:53013094-53013113 None:intergenic 45.0%
TCATTATTGCAGTGGCACTC+AGG + chr1_4:53013094-53013113 Msa0169730:CDS 45.0%
CATGGTGAAAACACCAGCTA+AGG - chr1_4:53013481-53013500 None:intergenic 45.0%
AGGTCCCTTGTATAGTGTCA+TGG - chr1_4:53013499-53013518 None:intergenic 45.0%
GCCCTATAATCCATGCTGAA+GGG - chr1_4:53013852-53013871 None:intergenic 45.0%
AGCCCTATAATCCATGCTGA+AGG - chr1_4:53013853-53013872 None:intergenic 45.0%
!! TAGGGCTCAACATTGACTTG+TGG + chr1_4:53013866-53013885 Msa0169730:CDS 45.0%
TGGGTTTGTATCTGTTGCTG+TGG + chr1_4:53014335-53014354 Msa0169730:CDS 45.0%
GGGTTTGTATCTGTTGCTGT+GGG + chr1_4:53014336-53014355 Msa0169730:CDS 45.0%
GGTTTGTATCTGTTGCTGTG+GGG + chr1_4:53014337-53014356 Msa0169730:CDS 45.0%
GTTTGTATCTGTTGCTGTGG+GGG + chr1_4:53014338-53014357 Msa0169730:CDS 45.0%
CTACCTCCGTCCCTAATTAT+AGG + chr1_4:53014731-53014750 Msa0169730:three_prime_UTR 45.0%
TGTCCTATAATTAGGGACGG+AGG - chr1_4:53014737-53014756 None:intergenic 45.0%
!!! ATTTGTATAATTTTAAAATA+TGG + chr1_4:53012973-53012992 Msa0169730:intron 5.0%
!! TAGTTTTGCAGTTGCGCACG+TGG - chr1_4:53012574-53012593 None:intergenic 50.0%
TCTGGGTTCGAATCCAGCAT+TGG - chr1_4:53012666-53012685 None:intergenic 50.0%
TGGATTCGAACCCAGAACCT+TGG + chr1_4:53012670-53012689 Msa0169730:intron 50.0%
GGGACCTTGATATCCTTAGC+TGG + chr1_4:53013465-53013484 Msa0169730:CDS 50.0%
! TGACAGGGAATTGTTGGTGG+TGG + chr1_4:53013997-53014016 Msa0169730:intron 50.0%
GGGGAAAGAAGGTGACAAAG+AGG + chr1_4:53014357-53014376 Msa0169730:CDS 50.0%
ACCTATCGGCATGTTCTCGC+CGG + chr1_4:53012415-53012434 Msa0169730:CDS 55.0%
TTGCGCACGTGGAGGATACT+CGG - chr1_4:53012563-53012582 None:intergenic 55.0%
! TTTTGCAGTTGCGCACGTGG+AGG - chr1_4:53012571-53012590 None:intergenic 55.0%
GGGAGAGTTTGCCGTCCATT+TGG - chr1_4:53012618-53012637 None:intergenic 55.0%
TGCTGCTGCATGTCCAATGC+TGG + chr1_4:53012650-53012669 Msa0169730:intron 55.0%
CTGTTGCTGTGGGGGAAAGA+AGG + chr1_4:53014346-53014365 Msa0169730:CDS 55.0%
CCTATCGGCATGTTCTCGCC+GGG + chr1_4:53012416-53012435 Msa0169730:CDS 60.0%
CTATCGGCATGTTCTCGCCG+GGG + chr1_4:53012417-53012436 Msa0169730:CDS 60.0%
CCCGGCGAGAACATGCCGAT+AGG - chr1_4:53012419-53012438 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 53012211 53014853 53012211 ID=Msa0169730;Name=Msa0169730
chr1_4 mRNA 53012211 53014853 53012211 ID=Msa0169730-mRNA-1;Parent=Msa0169730;Name=Msa0169730-mRNA-1;_AED=0.07;_eAED=0.07;_QI=57|1|1|1|1|1|7|412|356
chr1_4 exon 53012211 53012473 53012211 ID=Msa0169730-mRNA-1:exon:15828;Parent=Msa0169730-mRNA-1
chr1_4 exon 53012855 53012917 53012855 ID=Msa0169730-mRNA-1:exon:15829;Parent=Msa0169730-mRNA-1
chr1_4 exon 53012999 53013115 53012999 ID=Msa0169730-mRNA-1:exon:15830;Parent=Msa0169730-mRNA-1
chr1_4 exon 53013416 53013653 53013416 ID=Msa0169730-mRNA-1:exon:15831;Parent=Msa0169730-mRNA-1
chr1_4 exon 53013742 53013903 53013742 ID=Msa0169730-mRNA-1:exon:15832;Parent=Msa0169730-mRNA-1
chr1_4 exon 53014003 53014154 53014003 ID=Msa0169730-mRNA-1:exon:15833;Parent=Msa0169730-mRNA-1
chr1_4 exon 53014309 53014853 53014309 ID=Msa0169730-mRNA-1:exon:15834;Parent=Msa0169730-mRNA-1
chr1_4 five_prime_UTR 53012211 53012267 53012211 ID=Msa0169730-mRNA-1:five_prime_utr;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53012268 53012473 53012268 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53012855 53012917 53012855 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53012999 53013115 53012999 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53013416 53013653 53013416 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53013742 53013903 53013742 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53014003 53014154 53014003 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 CDS 53014309 53014441 53014309 ID=Msa0169730-mRNA-1:cds;Parent=Msa0169730-mRNA-1
chr1_4 three_prime_UTR 53014442 53014853 53014442 ID=Msa0169730-mRNA-1:three_prime_utr;Parent=Msa0169730-mRNA-1
Gene Sequence

>Msa0169730

ATGGAGAAAAAAGTACTTTCTCCTCTACATGTATACAAAGAGTTCAAGCCACACCTTATCATGGTTTTAGCCCAAGTTGGCTATACCTTTTTATATTTTATCTCTGAATCTTCCTTCAATCATGGAATGAGTCCTTATGTTTATGTTACCTATCGGCATGTTCTCGCCGGGGTTGTGATGTTTCCTTTTGCATACTTTCTTGAGAGAAGTTTAAGACCAAAGCTGACACTTGTTCTTTTTATGGAAATATTTGTGCTTTCTTTGTTAGGGATCACCATGGCACTCAATTTGTACTTTGCAAGCTTAAAGTATACTTCTCCAACATTTCTTGCTTCCATGTTCAACACTATAGCTTCCATTACCTTCATTATTGCAGTGGCACTCAGGATTGAGGTTATTGATCTTAGAAATCCACGTAGCATAGCAAAAATTCTTGGGACCTTGATATCCTTAGCTGGTGTTTTCACCATGACACTATACAAGGGACCTGTTATGAGAAACTTGTGGCATCCTCTAATCCATATTATTCAACCAAAAAGTTCTTCTATCAACGAGAGTGGATTAAAGGGTTCAATTCTTACTGTCTCATGTTGTGCCACGTTTTCTATATGGTACATTATGCAGGCATCAACTCTAAAAAGATACCCTGCTCAATTATCACTCACTACATGGATGTGCTTTATTGGAGCAGCACAATCAGCTATTTTCACAGTAATAGTAGAACATAATAACCCTTCAGCATGGATTATAGGGCTCAACATTGACTTGTGGTCCATAATATATGGGGGAATTGTTGGTGGTGGTTTATTGATCTACATTCAATTATGGTGCACTGAGAAAAAAGGGCCTGTTTTTGTCACTGTATTTAACCCTCTTTGTACTATATTTGTGGCAATACTAGCATACTTCGTCCTTGGTGAAAAACTTTATCTGGGCAGCATCATAGGTGCATTTATTGTCATCATGGGTTTGTATCTGTTGCTGTGGGGGAAAGAAGGTGACAAAGAGGTCGATTTCAAGACCAAAGTTAAAAGGCAGTACAAAAATGGAGGAGACCTAGAAGAATGCTGA
Protein sequence

>Msa0169730

MEKKVLSPLHVYKEFKPHLIMVLAQVGYTFLYFISESSFNHGMSPYVYVTYRHVLAGVVMFPFAYFLERSLRPKLTLVLFMEIFVLSLLGITMALNLYFASLKYTSPTFLASMFNTIASITFIIAVALRIEVIDLRNPRSIAKILGTLISLAGVFTMTLYKGPVMRNLWHPLIHIIQPKSSSINESGLKGSILTVSCCATFSIWYIMQASTLKRYPAQLSLTTWMCFIGAAQSAIFTVIVEHNNPSAWIIGLNIDLWSIIYGGIVGGGLLIYIQLWCTEKKGPVFVTVFNPLCTIFVAILAYFVLGEKLYLGSIIGAFIVIMGLYLLLWGKEGDKEVDFKTKVKRQYKNGGDLEEC*