AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0082220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082220 MtrunA17_Chr1g0195681 99.412 170 1 0 1 170 1 170 7.63e-123 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0082220 AT1G02870.1 56.477 193 60 4 1 169 1 193 6.17e-68 205

Find 59 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 1_2:+61026296 Msa0082220:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 1_2:-61024831 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 1_2:-61024970 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 1_2:+61025019 Msa0082220:CDS
GAGCCTTTATCGTCCCATTT+AGG 0.265744 1_2:-61025033 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 1_2:+61025287 Msa0082220:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 1_2:-61025003 None:intergenic
CGCCCAGATTGTAAATTTGA+TGG 0.304006 1_2:-61026243 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 1_2:-61025062 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 1_2:-61024958 None:intergenic
TTGGGAAAGATGTAGTATTC+TGG 0.325763 1_2:+61026438 Msa0082220:three_prime_UTR
GAGTATGAGATTAGGGTTTA+GGG 0.339426 1_2:-61024830 None:intergenic
TGGAATGTGATAAGTTGTTT+TGG 0.344430 1_2:+61026512 Msa0082220:three_prime_UTR
ACCCAAAACTATTCCTCTTT+CGG 0.353542 1_2:+61025060 Msa0082220:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 1_2:-61024937 None:intergenic
ATGCCATCAAATTTACAATC+TGG 0.368534 1_2:+61026240 Msa0082220:three_prime_UTR
TGCAACTGCGTAAGCAAGAT+AGG 0.370438 1_2:-61024860 None:intergenic
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 1_2:-61025096 None:intergenic
GACAAGAATCCGCTCATTGT+TGG 0.413347 1_2:+61026185 Msa0082220:CDS
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 1_2:+61026297 Msa0082220:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 1_2:-61024969 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 1_2:-61024936 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 1_2:+61025030 Msa0082220:CDS
GACACAGAGAGAAGAAGAAT+GGG 0.476563 1_2:+61024883 Msa0082220:exon
GAATTTGAAGAGAATCAGTA+AGG 0.477015 1_2:-61025104 None:intergenic
TGCCATCAAATTTACAATCT+GGG 0.479882 1_2:+61026241 Msa0082220:three_prime_UTR
GTCTTCTTCAAGATCACTGC+CGG 0.487579 1_2:-61025151 None:intergenic
TTGTGGTGTAGATTTGAAAT+CGG 0.497171 1_2:+61025273 Msa0082220:intron
GTAGATTTGAAATCGGCGTT+GGG 0.505626 1_2:+61025280 Msa0082220:intron
AGACACAGAGAGAAGAAGAA+TGG 0.507644 1_2:+61024882 Msa0082220:exon
TCCATGCAAAATTCAAAAGC+AGG 0.510757 1_2:-61026409 None:intergenic
TCAAATTTACAATCTGGGCG+TGG 0.510966 1_2:+61026246 Msa0082220:three_prime_UTR
ATGGCATTGCACTTGTCCAT+AGG 0.520593 1_2:-61026224 None:intergenic
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 1_2:-61025334 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 1_2:+61025279 Msa0082220:intron
ATCAGAAACAACGCCGAAAG+AGG 0.531608 1_2:-61025073 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 1_2:+61026263 Msa0082220:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 1_2:-61026125 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 1_2:-61026124 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 1_2:+61025020 Msa0082220:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 1_2:-61025019 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 1_2:-61026291 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 1_2:-61025020 None:intergenic
GTTGCCGATGATAACGATCC+CGG 0.559862 1_2:+61025132 Msa0082220:CDS
CAAATTTACAATCTGGGCGT+GGG 0.560830 1_2:+61026247 Msa0082220:three_prime_UTR
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 1_2:-61024985 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 1_2:-61025150 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 1_2:-61025126 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.606004 1_2:-61024859 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 1_2:-61025340 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 1_2:+61026274 Msa0082220:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 1_2:-61025103 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 1_2:+61025298 Msa0082220:CDS
TTTCATTTACCAACAATGAG+CGG 0.625317 1_2:-61026194 None:intergenic
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 1_2:+61025153 Msa0082220:CDS
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 1_2:+61024931 Msa0082220:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 1_2:+61024921 Msa0082220:CDS
AGCAAAATGTTGTTGCAATG+AGG 0.658596 1_2:+61026327 Msa0082220:three_prime_UTR
ACTGCCGGGATCGTTATCAT+CGG 0.664050 1_2:-61025136 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG + chr1_2:61026072-61026091 Msa0082220:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG + chr1_2:61026072-61026091 Msa0082220:intron 10.0%
!!! TTAAAACTTTTGAAAATGAA+CGG + chr1_2:61025610-61025629 Msa0082220:intron 15.0%
!! AACTAATTTATTTGCATAAA+CGG - chr1_2:61025734-61025753 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - chr1_2:61026482-61026501 None:intergenic 15.0%
!!! TTAAAACTTTTGAAAATGAA+CGG + chr1_2:61025610-61025629 Msa0082220:intron 15.0%
!! AACTAATTTATTTGCATAAA+CGG - chr1_2:61025734-61025753 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - chr1_2:61026482-61026501 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG + chr1_2:61025212-61025231 Msa0082220:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + chr1_2:61025246-61025265 Msa0082220:intron 20.0%
!!! TGTTTTTTCATAAATTACCT+TGG - chr1_2:61025780-61025799 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + chr1_2:61026297-61026316 Msa0082220:three_prime_UTR 20.0%
!! GTTGAAATTAGTATTAATCT+TGG + chr1_2:61025212-61025231 Msa0082220:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + chr1_2:61025246-61025265 Msa0082220:intron 20.0%
!!! TGTTTTTTCATAAATTACCT+TGG - chr1_2:61025780-61025799 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + chr1_2:61026297-61026316 Msa0082220:three_prime_UTR 20.0%
! AATTTGAAGAGAATCAGTAA+GGG - chr1_2:61025106-61025125 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - chr1_2:61025185-61025204 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + chr1_2:61025224-61025243 Msa0082220:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + chr1_2:61025370-61025389 Msa0082220:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + chr1_2:61025384-61025403 Msa0082220:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - chr1_2:61025483-61025502 None:intergenic 25.0%
!!! TTTATCTTTTGCTATAGAAG+TGG - chr1_2:61025550-61025569 None:intergenic 25.0%
!!! TAAGTTTTGTGAGATTGTTT+GGG - chr1_2:61025664-61025683 None:intergenic 25.0%
!!! ATAAGTTTTGTGAGATTGTT+TGG - chr1_2:61025665-61025684 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + chr1_2:61025788-61025807 Msa0082220:intron 25.0%
!! CATTTTACTCATATACTACA+TGG + chr1_2:61025864-61025883 Msa0082220:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG + chr1_2:61025920-61025939 Msa0082220:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + chr1_2:61026208-61026227 Msa0082220:exon 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + chr1_2:61026296-61026315 Msa0082220:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + chr1_2:61026419-61026438 Msa0082220:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + chr1_2:61026420-61026439 Msa0082220:three_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG - chr1_2:61026467-61026486 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG - chr1_2:61026468-61026487 None:intergenic 25.0%
!! TAGATGAAAGTAGTTTTTGA+TGG + chr1_2:61026492-61026511 Msa0082220:three_prime_UTR 25.0%
! AATTTGAAGAGAATCAGTAA+GGG - chr1_2:61025106-61025125 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - chr1_2:61025185-61025204 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + chr1_2:61025224-61025243 Msa0082220:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + chr1_2:61025370-61025389 Msa0082220:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + chr1_2:61025384-61025403 Msa0082220:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - chr1_2:61025483-61025502 None:intergenic 25.0%
!!! TTTATCTTTTGCTATAGAAG+TGG - chr1_2:61025550-61025569 None:intergenic 25.0%
!!! TAAGTTTTGTGAGATTGTTT+GGG - chr1_2:61025664-61025683 None:intergenic 25.0%
!!! ATAAGTTTTGTGAGATTGTT+TGG - chr1_2:61025665-61025684 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + chr1_2:61025788-61025807 Msa0082220:intron 25.0%
!! CATTTTACTCATATACTACA+TGG + chr1_2:61025864-61025883 Msa0082220:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG + chr1_2:61025920-61025939 Msa0082220:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + chr1_2:61026208-61026227 Msa0082220:exon 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + chr1_2:61026296-61026315 Msa0082220:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + chr1_2:61026419-61026438 Msa0082220:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + chr1_2:61026420-61026439 Msa0082220:three_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG - chr1_2:61026467-61026486 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG - chr1_2:61026468-61026487 None:intergenic 25.0%
!! TAGATGAAAGTAGTTTTTGA+TGG + chr1_2:61026492-61026511 Msa0082220:three_prime_UTR 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - chr1_2:61024840-61024859 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - chr1_2:61025107-61025126 None:intergenic 30.0%
! AATTTGAGTATGGTGTTTTG+TGG + chr1_2:61025256-61025275 Msa0082220:intron 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + chr1_2:61025273-61025292 Msa0082220:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + chr1_2:61025760-61025779 Msa0082220:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG + chr1_2:61025803-61025822 Msa0082220:intron 30.0%
TACTCATATACTACATGGTT+AGG + chr1_2:61025869-61025888 Msa0082220:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + chr1_2:61025959-61025978 Msa0082220:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + chr1_2:61026023-61026042 Msa0082220:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + chr1_2:61026030-61026049 Msa0082220:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + chr1_2:61026045-61026064 Msa0082220:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + chr1_2:61026088-61026107 Msa0082220:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - chr1_2:61026197-61026216 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG + chr1_2:61026240-61026259 Msa0082220:three_prime_UTR 30.0%
TGCCATCAAATTTACAATCT+GGG + chr1_2:61026241-61026260 Msa0082220:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - chr1_2:61026294-61026313 None:intergenic 30.0%
! TGGAATGTGATAAGTTGTTT+TGG + chr1_2:61026512-61026531 Msa0082220:three_prime_UTR 30.0%
!! GGAATGTGATAAGTTGTTTT+GGG + chr1_2:61026513-61026532 Msa0082220:three_prime_UTR 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - chr1_2:61024840-61024859 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - chr1_2:61025107-61025126 None:intergenic 30.0%
! AATTTGAGTATGGTGTTTTG+TGG + chr1_2:61025256-61025275 Msa0082220:intron 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + chr1_2:61025273-61025292 Msa0082220:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + chr1_2:61025760-61025779 Msa0082220:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG + chr1_2:61025803-61025822 Msa0082220:intron 30.0%
TACTCATATACTACATGGTT+AGG + chr1_2:61025869-61025888 Msa0082220:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + chr1_2:61025959-61025978 Msa0082220:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + chr1_2:61026023-61026042 Msa0082220:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + chr1_2:61026030-61026049 Msa0082220:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + chr1_2:61026045-61026064 Msa0082220:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + chr1_2:61026088-61026107 Msa0082220:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - chr1_2:61026197-61026216 None:intergenic 30.0%
ATGCCATCAAATTTACAATC+TGG + chr1_2:61026240-61026259 Msa0082220:three_prime_UTR 30.0%
TGCCATCAAATTTACAATCT+GGG + chr1_2:61026241-61026260 Msa0082220:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - chr1_2:61026294-61026313 None:intergenic 30.0%
! TGGAATGTGATAAGTTGTTT+TGG + chr1_2:61026512-61026531 Msa0082220:three_prime_UTR 30.0%
GAGTATGAGATTAGGGTTTA+GGG - chr1_2:61024833-61024852 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - chr1_2:61024841-61024860 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - chr1_2:61024961-61024980 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - chr1_2:61024989-61025008 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + chr1_2:61025060-61025079 Msa0082220:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - chr1_2:61025337-61025356 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + chr1_2:61025409-61025428 Msa0082220:intron 35.0%
! TGCTTTGATAGTTCACTTCT+AGG + chr1_2:61025456-61025475 Msa0082220:intron 35.0%
TCTCACAAAACTTATGTCAG+TGG + chr1_2:61025670-61025689 Msa0082220:intron 35.0%
AGCAAAATGTTGTTGCAATG+AGG + chr1_2:61026327-61026346 Msa0082220:three_prime_UTR 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + chr1_2:61026408-61026427 Msa0082220:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - chr1_2:61026412-61026431 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + chr1_2:61026438-61026457 Msa0082220:three_prime_UTR 35.0%
GAGTATGAGATTAGGGTTTA+GGG - chr1_2:61024833-61024852 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - chr1_2:61024841-61024860 None:intergenic 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - chr1_2:61024961-61024980 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - chr1_2:61024989-61025008 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + chr1_2:61025060-61025079 Msa0082220:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - chr1_2:61025337-61025356 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + chr1_2:61025409-61025428 Msa0082220:intron 35.0%
! TGCTTTGATAGTTCACTTCT+AGG + chr1_2:61025456-61025475 Msa0082220:intron 35.0%
TCTCACAAAACTTATGTCAG+TGG + chr1_2:61025670-61025689 Msa0082220:intron 35.0%
AGCAAAATGTTGTTGCAATG+AGG + chr1_2:61026327-61026346 Msa0082220:three_prime_UTR 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + chr1_2:61026408-61026427 Msa0082220:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - chr1_2:61026412-61026431 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + chr1_2:61026438-61026457 Msa0082220:three_prime_UTR 35.0%
CGAGTATGAGATTAGGGTTT+AGG - chr1_2:61024834-61024853 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG + chr1_2:61024882-61024901 Msa0082220:exon 40.0%
GACACAGAGAGAAGAAGAAT+GGG + chr1_2:61024883-61024902 Msa0082220:exon 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - chr1_2:61024972-61024991 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - chr1_2:61024973-61024992 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - chr1_2:61024988-61025007 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - chr1_2:61025003-61025022 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - chr1_2:61025006-61025025 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - chr1_2:61025022-61025041 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + chr1_2:61025020-61025039 Msa0082220:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + chr1_2:61025030-61025049 Msa0082220:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - chr1_2:61025064-61025083 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - chr1_2:61025099-61025118 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + chr1_2:61025279-61025298 Msa0082220:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + chr1_2:61025280-61025299 Msa0082220:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + chr1_2:61025298-61025317 Msa0082220:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - chr1_2:61025343-61025362 None:intergenic 40.0%
CCTGTCCCAATAAACCTTTA+GGG + chr1_2:61025708-61025727 Msa0082220:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - chr1_2:61025716-61025735 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - chr1_2:61025717-61025736 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - chr1_2:61025725-61025744 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + chr1_2:61025975-61025994 Msa0082220:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - chr1_2:61026127-61026146 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG - chr1_2:61026246-61026265 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG + chr1_2:61026246-61026265 Msa0082220:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + chr1_2:61026247-61026266 Msa0082220:three_prime_UTR 40.0%
CGAGTATGAGATTAGGGTTT+AGG - chr1_2:61024834-61024853 None:intergenic 40.0%
AGACACAGAGAGAAGAAGAA+TGG + chr1_2:61024882-61024901 Msa0082220:exon 40.0%
GACACAGAGAGAAGAAGAAT+GGG + chr1_2:61024883-61024902 Msa0082220:exon 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - chr1_2:61024972-61024991 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - chr1_2:61024973-61024992 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - chr1_2:61024988-61025007 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - chr1_2:61025003-61025022 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - chr1_2:61025006-61025025 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - chr1_2:61025022-61025041 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + chr1_2:61025020-61025039 Msa0082220:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + chr1_2:61025030-61025049 Msa0082220:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - chr1_2:61025064-61025083 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - chr1_2:61025099-61025118 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + chr1_2:61025279-61025298 Msa0082220:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + chr1_2:61025280-61025299 Msa0082220:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + chr1_2:61025298-61025317 Msa0082220:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - chr1_2:61025343-61025362 None:intergenic 40.0%
CCTGTCCCAATAAACCTTTA+GGG + chr1_2:61025708-61025727 Msa0082220:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - chr1_2:61025716-61025735 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - chr1_2:61025717-61025736 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - chr1_2:61025725-61025744 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + chr1_2:61025975-61025994 Msa0082220:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - chr1_2:61026127-61026146 None:intergenic 40.0%
CGCCCAGATTGTAAATTTGA+TGG - chr1_2:61026246-61026265 None:intergenic 40.0%
TCAAATTTACAATCTGGGCG+TGG + chr1_2:61026246-61026265 Msa0082220:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + chr1_2:61026247-61026266 Msa0082220:three_prime_UTR 40.0%
GCAACTGCGTAAGCAAGATA+GGG - chr1_2:61024862-61024881 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - chr1_2:61024863-61024882 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + chr1_2:61024921-61024940 Msa0082220:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - chr1_2:61024939-61024958 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + chr1_2:61025019-61025038 Msa0082220:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - chr1_2:61025023-61025042 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - chr1_2:61025036-61025055 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - chr1_2:61025065-61025084 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - chr1_2:61025076-61025095 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - chr1_2:61025129-61025148 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - chr1_2:61025153-61025172 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - chr1_2:61025154-61025173 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + chr1_2:61025153-61025172 Msa0082220:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + chr1_2:61025287-61025306 Msa0082220:CDS 45.0%
GTTCACTTCTAGGTCCTGTT+TGG + chr1_2:61025466-61025485 Msa0082220:intron 45.0%
GCCTGTCCCAATAAACCTTT+AGG + chr1_2:61025707-61025726 Msa0082220:intron 45.0%
CCCTAAAGGTTTATTGGGAC+AGG - chr1_2:61025711-61025730 None:intergenic 45.0%
GTTGCAACTGAATGCTGCAT+AGG - chr1_2:61026128-61026147 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + chr1_2:61026185-61026204 Msa0082220:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG - chr1_2:61026227-61026246 None:intergenic 45.0%
GCAACTGCGTAAGCAAGATA+GGG - chr1_2:61024862-61024881 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - chr1_2:61024863-61024882 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + chr1_2:61024921-61024940 Msa0082220:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - chr1_2:61024939-61024958 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + chr1_2:61025019-61025038 Msa0082220:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - chr1_2:61025023-61025042 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - chr1_2:61025036-61025055 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - chr1_2:61025065-61025084 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - chr1_2:61025076-61025095 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - chr1_2:61025129-61025148 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - chr1_2:61025153-61025172 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - chr1_2:61025154-61025173 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + chr1_2:61025153-61025172 Msa0082220:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + chr1_2:61025287-61025306 Msa0082220:CDS 45.0%
GTTCACTTCTAGGTCCTGTT+TGG + chr1_2:61025466-61025485 Msa0082220:intron 45.0%
GCCTGTCCCAATAAACCTTT+AGG + chr1_2:61025707-61025726 Msa0082220:intron 45.0%
CCCTAAAGGTTTATTGGGAC+AGG - chr1_2:61025711-61025730 None:intergenic 45.0%
GTTGCAACTGAATGCTGCAT+AGG - chr1_2:61026128-61026147 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + chr1_2:61026185-61026204 Msa0082220:CDS 45.0%
ATGGCATTGCACTTGTCCAT+AGG - chr1_2:61026227-61026246 None:intergenic 45.0%
GGAAGACCAACACGTACCTT+TGG - chr1_2:61024940-61024959 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + chr1_2:61025132-61025151 Msa0082220:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - chr1_2:61025139-61025158 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG - chr1_2:61024940-61024959 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + chr1_2:61025132-61025151 Msa0082220:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - chr1_2:61025139-61025158 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG + chr1_2:61024931-61024950 Msa0082220:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + chr1_2:61026274-61026293 Msa0082220:three_prime_UTR 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG + chr1_2:61024931-61024950 Msa0082220:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + chr1_2:61026274-61026293 Msa0082220:three_prime_UTR 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + chr1_2:61026263-61026282 Msa0082220:three_prime_UTR 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + chr1_2:61026263-61026282 Msa0082220:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 61024799 61026535 61024799 ID=Msa0082220;Name=Msa0082220
chr1_2 mRNA 61024799 61026535 61024799 ID=Msa0082220-mRNA-1;Parent=Msa0082220;Name=Msa0082220-mRNA-1;_AED=0.01;_eAED=0.01;_QI=102|1|1|1|1|1|3|321|170
chr1_2 exon 61024799 61025174 61024799 ID=Msa0082220-mRNA-1:exon:20015;Parent=Msa0082220-mRNA-1
chr1_2 exon 61025284 61025405 61025284 ID=Msa0082220-mRNA-1:exon:20016;Parent=Msa0082220-mRNA-1
chr1_2 exon 61026098 61026535 61026098 ID=Msa0082220-mRNA-1:exon:20017;Parent=Msa0082220-mRNA-1
chr1_2 five_prime_UTR 61024799 61024900 61024799 ID=Msa0082220-mRNA-1:five_prime_utr;Parent=Msa0082220-mRNA-1
chr1_2 CDS 61024901 61025174 61024901 ID=Msa0082220-mRNA-1:cds;Parent=Msa0082220-mRNA-1
chr1_2 CDS 61025284 61025405 61025284 ID=Msa0082220-mRNA-1:cds;Parent=Msa0082220-mRNA-1
chr1_2 CDS 61026098 61026214 61026098 ID=Msa0082220-mRNA-1:cds;Parent=Msa0082220-mRNA-1
chr1_2 three_prime_UTR 61026215 61026535 61026215 ID=Msa0082220-mRNA-1:three_prime_utr;Parent=Msa0082220-mRNA-1
Gene Sequence

>Msa0082220

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATAAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa0082220

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYKDKNPLIVGK*