AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1370800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370800 MtrunA17_Chr1g0195681 99.412 170 1 0 1 170 1 170 7.63e-123 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1370800 AT1G02870.1 56.477 193 60 4 1 169 1 193 6.17e-68 205

Find 58 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 tig0019143:+117820 Msa1370800:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 tig0019143:-116372 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 tig0019143:-116511 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 tig0019143:+116560 Msa1370800:CDS
TTAATAGATGAAAGTAGTTT+TGG 0.244162 tig0019143:+118012 Msa1370800:three_prime_UTR
GAGCCTTTATCGTCCCATTT+AGG 0.265744 tig0019143:-116574 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 tig0019143:+116826 Msa1370800:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 tig0019143:-116544 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 tig0019143:-116603 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 tig0019143:-116499 None:intergenic
TTGGGAAAGATGTAGTATTC+TGG 0.325763 tig0019143:+117962 Msa1370800:three_prime_UTR
GAGTATGAGATTAGGGTTTA+GGG 0.339426 tig0019143:-116371 None:intergenic
ACCCAAAACTATTCCTCTTT+CGG 0.353542 tig0019143:+116601 Msa1370800:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 tig0019143:-116478 None:intergenic
TGCAACTGCGTAAGCAAGAT+AGG 0.370438 tig0019143:-116401 None:intergenic
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 tig0019143:-116637 None:intergenic
GACAAGAATCCGCTCATTGT+TGG 0.413347 tig0019143:+117724 Msa1370800:CDS
CTATGGACAAATTTACAATC+TGG 0.413457 tig0019143:+117764 Msa1370800:three_prime_UTR
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 tig0019143:+117821 Msa1370800:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 tig0019143:-116510 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 tig0019143:-116477 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 tig0019143:+116571 Msa1370800:CDS
GACACAAAGAGAAGAAGAAT+GGG 0.456584 tig0019143:+116424 Msa1370800:exon
TTGTGGTGTAGATTTGAAAT+CGG 0.469021 tig0019143:+116812 Msa1370800:intron
GAATTTGAAGAGAATCAGTA+AGG 0.477015 tig0019143:-116645 None:intergenic
GTCTTCTTCAAGATCACTGC+CGG 0.487579 tig0019143:-116692 None:intergenic
AGACACAAAGAGAAGAAGAA+TGG 0.504926 tig0019143:+116423 Msa1370800:exon
GTAGATTTGAAATCGGCGTT+GGG 0.505626 tig0019143:+116819 Msa1370800:intron
TCCATGCAAAATTCAAAAGC+AGG 0.510757 tig0019143:-117933 None:intergenic
TATGGACAAATTTACAATCT+GGG 0.511461 tig0019143:+117765 Msa1370800:three_prime_UTR
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 tig0019143:-116873 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 tig0019143:+116818 Msa1370800:intron
ACAAATTTACAATCTGGGCG+TGG 0.531570 tig0019143:+117770 Msa1370800:three_prime_UTR
ATCAGAAACAACGCCGAAAG+AGG 0.531608 tig0019143:-116614 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 tig0019143:+117787 Msa1370800:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 tig0019143:-117664 None:intergenic
CAGATTGTAAATTTGTCCAT+AGG 0.535480 tig0019143:-117763 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 tig0019143:-117663 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 tig0019143:+116561 Msa1370800:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 tig0019143:-116560 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 tig0019143:-117815 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 tig0019143:-116561 None:intergenic
CAAATTTACAATCTGGGCGT+GGG 0.557585 tig0019143:+117771 Msa1370800:three_prime_UTR
GTTGCCGATGATAACGATCC+CGG 0.559862 tig0019143:+116673 Msa1370800:CDS
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 tig0019143:-116526 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 tig0019143:-116691 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 tig0019143:-116667 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.606004 tig0019143:-116400 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 tig0019143:-116879 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 tig0019143:+117798 Msa1370800:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 tig0019143:-116644 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 tig0019143:+116837 Msa1370800:CDS
TTTCATTTACCAACAATGAG+CGG 0.625317 tig0019143:-117733 None:intergenic
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 tig0019143:+116694 Msa1370800:CDS
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 tig0019143:+116472 Msa1370800:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 tig0019143:+116462 Msa1370800:CDS
ACTGCCGGGATCGTTATCAT+CGG 0.664050 tig0019143:-116677 None:intergenic
AGCAAAATGTTGTTACAATG+AGG 0.667627 tig0019143:+117851 Msa1370800:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG + tig0019143:117611-117630 Msa1370800:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG + tig0019143:117611-117630 Msa1370800:intron 10.0%
!! AACTAATTTATTTGCATAAA+CGG - tig0019143:117273-117292 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - tig0019143:118006-118025 None:intergenic 15.0%
!! AACTAATTTATTTGCATAAA+CGG - tig0019143:117273-117292 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG - tig0019143:118006-118025 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG + tig0019143:116753-116772 Msa1370800:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + tig0019143:116787-116806 Msa1370800:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG - tig0019143:117089-117108 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG - tig0019143:117203-117222 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG - tig0019143:117204-117223 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG - tig0019143:117319-117338 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + tig0019143:117821-117840 Msa1370800:three_prime_UTR 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG + tig0019143:118012-118031 Msa1370800:three_prime_UTR 20.0%
!! GTTGAAATTAGTATTAATCT+TGG + tig0019143:116753-116772 Msa1370800:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG + tig0019143:116787-116806 Msa1370800:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG - tig0019143:117089-117108 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG - tig0019143:117203-117222 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG - tig0019143:117204-117223 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG - tig0019143:117319-117338 None:intergenic 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG + tig0019143:117821-117840 Msa1370800:three_prime_UTR 20.0%
! AATTTGAAGAGAATCAGTAA+GGG - tig0019143:116647-116666 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - tig0019143:116726-116745 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + tig0019143:116765-116784 Msa1370800:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG + tig0019143:116795-116814 Msa1370800:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + tig0019143:116909-116928 Msa1370800:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + tig0019143:116923-116942 Msa1370800:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - tig0019143:117022-117041 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + tig0019143:117327-117346 Msa1370800:intron 25.0%
!! CATTTTACTCATATACTACA+TGG + tig0019143:117403-117422 Msa1370800:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG + tig0019143:117459-117478 Msa1370800:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + tig0019143:117747-117766 Msa1370800:exon 25.0%
! TATGGACAAATTTACAATCT+GGG + tig0019143:117765-117784 Msa1370800:three_prime_UTR 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + tig0019143:117820-117839 Msa1370800:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + tig0019143:117943-117962 Msa1370800:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + tig0019143:117944-117963 Msa1370800:three_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG - tig0019143:117991-118010 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG - tig0019143:117992-118011 None:intergenic 25.0%
! AATTTGAAGAGAATCAGTAA+GGG - tig0019143:116647-116666 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG - tig0019143:116726-116745 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG + tig0019143:116765-116784 Msa1370800:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG + tig0019143:116795-116814 Msa1370800:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG + tig0019143:116909-116928 Msa1370800:CDS 25.0%
!! AAAATATGGAGATGATTTTC+AGG + tig0019143:116923-116942 Msa1370800:CDS 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG - tig0019143:117022-117041 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG + tig0019143:117327-117346 Msa1370800:intron 25.0%
!! CATTTTACTCATATACTACA+TGG + tig0019143:117403-117422 Msa1370800:intron 25.0%
! GTATCATAATCTTGAAACAT+CGG + tig0019143:117459-117478 Msa1370800:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG + tig0019143:117747-117766 Msa1370800:exon 25.0%
! TATGGACAAATTTACAATCT+GGG + tig0019143:117765-117784 Msa1370800:three_prime_UTR 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG + tig0019143:117820-117839 Msa1370800:three_prime_UTR 25.0%
!! AATTTTGCATGGATTTTTGT+TGG + tig0019143:117943-117962 Msa1370800:three_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG + tig0019143:117944-117963 Msa1370800:three_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG - tig0019143:117991-118010 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG - tig0019143:117992-118011 None:intergenic 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - tig0019143:116381-116400 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - tig0019143:116648-116667 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + tig0019143:116812-116831 Msa1370800:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG + tig0019143:117209-117228 Msa1370800:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + tig0019143:117299-117318 Msa1370800:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG + tig0019143:117342-117361 Msa1370800:intron 30.0%
TACTCATATACTACATGGTT+AGG + tig0019143:117408-117427 Msa1370800:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + tig0019143:117498-117517 Msa1370800:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + tig0019143:117562-117581 Msa1370800:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + tig0019143:117569-117588 Msa1370800:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + tig0019143:117584-117603 Msa1370800:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + tig0019143:117627-117646 Msa1370800:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - tig0019143:117736-117755 None:intergenic 30.0%
CAGATTGTAAATTTGTCCAT+AGG - tig0019143:117766-117785 None:intergenic 30.0%
CTATGGACAAATTTACAATC+TGG + tig0019143:117764-117783 Msa1370800:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - tig0019143:117818-117837 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG + tig0019143:117851-117870 Msa1370800:three_prime_UTR 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG - tig0019143:116381-116400 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG - tig0019143:116648-116667 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG + tig0019143:116812-116831 Msa1370800:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG + tig0019143:117209-117228 Msa1370800:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG + tig0019143:117299-117318 Msa1370800:intron 30.0%
!! TATGAAGGTACAATTTTCGT+CGG + tig0019143:117342-117361 Msa1370800:intron 30.0%
TACTCATATACTACATGGTT+AGG + tig0019143:117408-117427 Msa1370800:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG + tig0019143:117498-117517 Msa1370800:intron 30.0%
! CAAATGTTACTCTGTTTTCT+TGG + tig0019143:117562-117581 Msa1370800:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG + tig0019143:117569-117588 Msa1370800:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG + tig0019143:117584-117603 Msa1370800:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG + tig0019143:117627-117646 Msa1370800:intron 30.0%
TTTCATTTACCAACAATGAG+CGG - tig0019143:117736-117755 None:intergenic 30.0%
CAGATTGTAAATTTGTCCAT+AGG - tig0019143:117766-117785 None:intergenic 30.0%
CTATGGACAAATTTACAATC+TGG + tig0019143:117764-117783 Msa1370800:three_prime_UTR 30.0%
! TAACTTTAGAAAGCTATCCA+TGG - tig0019143:117818-117837 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG + tig0019143:117851-117870 Msa1370800:three_prime_UTR 30.0%
GAGTATGAGATTAGGGTTTA+GGG - tig0019143:116374-116393 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - tig0019143:116382-116401 None:intergenic 35.0%
AGACACAAAGAGAAGAAGAA+TGG + tig0019143:116423-116442 Msa1370800:exon 35.0%
GACACAAAGAGAAGAAGAAT+GGG + tig0019143:116424-116443 Msa1370800:exon 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - tig0019143:116502-116521 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - tig0019143:116530-116549 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + tig0019143:116601-116620 Msa1370800:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - tig0019143:116876-116895 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + tig0019143:116948-116967 Msa1370800:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG + tig0019143:116995-117014 Msa1370800:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG + tig0019143:117247-117266 Msa1370800:intron 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + tig0019143:117932-117951 Msa1370800:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - tig0019143:117936-117955 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + tig0019143:117962-117981 Msa1370800:three_prime_UTR 35.0%
GAGTATGAGATTAGGGTTTA+GGG - tig0019143:116374-116393 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG - tig0019143:116382-116401 None:intergenic 35.0%
AGACACAAAGAGAAGAAGAA+TGG + tig0019143:116423-116442 Msa1370800:exon 35.0%
GACACAAAGAGAAGAAGAAT+GGG + tig0019143:116424-116443 Msa1370800:exon 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG - tig0019143:116502-116521 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG - tig0019143:116530-116549 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG + tig0019143:116601-116620 Msa1370800:CDS 35.0%
TGAATTGAAGTCAAAGGTTG+AGG - tig0019143:116876-116895 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG + tig0019143:116948-116967 Msa1370800:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG + tig0019143:116995-117014 Msa1370800:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG + tig0019143:117247-117266 Msa1370800:intron 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG + tig0019143:117932-117951 Msa1370800:three_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG - tig0019143:117936-117955 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG + tig0019143:117962-117981 Msa1370800:three_prime_UTR 35.0%
CGAGTATGAGATTAGGGTTT+AGG - tig0019143:116375-116394 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - tig0019143:116513-116532 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - tig0019143:116514-116533 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - tig0019143:116529-116548 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - tig0019143:116544-116563 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - tig0019143:116547-116566 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - tig0019143:116563-116582 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + tig0019143:116561-116580 Msa1370800:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + tig0019143:116571-116590 Msa1370800:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - tig0019143:116605-116624 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - tig0019143:116640-116659 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + tig0019143:116818-116837 Msa1370800:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + tig0019143:116819-116838 Msa1370800:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + tig0019143:116837-116856 Msa1370800:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - tig0019143:116882-116901 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG + tig0019143:117246-117265 Msa1370800:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - tig0019143:117255-117274 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - tig0019143:117256-117275 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - tig0019143:117264-117283 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + tig0019143:117514-117533 Msa1370800:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - tig0019143:117666-117685 None:intergenic 40.0%
ACAAATTTACAATCTGGGCG+TGG + tig0019143:117770-117789 Msa1370800:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + tig0019143:117771-117790 Msa1370800:three_prime_UTR 40.0%
CGAGTATGAGATTAGGGTTT+AGG - tig0019143:116375-116394 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG - tig0019143:116513-116532 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG - tig0019143:116514-116533 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG - tig0019143:116529-116548 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG - tig0019143:116544-116563 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG - tig0019143:116547-116566 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG - tig0019143:116563-116582 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG + tig0019143:116561-116580 Msa1370800:CDS 40.0%
GATCCTAAATGGGACGATAA+AGG + tig0019143:116571-116590 Msa1370800:CDS 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG - tig0019143:116605-116624 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG - tig0019143:116640-116659 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG + tig0019143:116818-116837 Msa1370800:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG + tig0019143:116819-116838 Msa1370800:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG + tig0019143:116837-116856 Msa1370800:CDS 40.0%
AGACGCTGAATTGAAGTCAA+AGG - tig0019143:116882-116901 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG + tig0019143:117246-117265 Msa1370800:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG - tig0019143:117255-117274 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG - tig0019143:117256-117275 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG - tig0019143:117264-117283 None:intergenic 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG + tig0019143:117514-117533 Msa1370800:intron 40.0%
TTGCAACTGAATGCTGCATA+GGG - tig0019143:117666-117685 None:intergenic 40.0%
ACAAATTTACAATCTGGGCG+TGG + tig0019143:117770-117789 Msa1370800:three_prime_UTR 40.0%
CAAATTTACAATCTGGGCGT+GGG + tig0019143:117771-117790 Msa1370800:three_prime_UTR 40.0%
GCAACTGCGTAAGCAAGATA+GGG - tig0019143:116403-116422 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - tig0019143:116404-116423 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + tig0019143:116462-116481 Msa1370800:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - tig0019143:116480-116499 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + tig0019143:116560-116579 Msa1370800:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - tig0019143:116564-116583 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - tig0019143:116577-116596 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - tig0019143:116606-116625 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - tig0019143:116617-116636 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - tig0019143:116670-116689 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - tig0019143:116694-116713 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - tig0019143:116695-116714 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + tig0019143:116694-116713 Msa1370800:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + tig0019143:116826-116845 Msa1370800:CDS 45.0%
GTTCACTTCTAGGTCCTGTT+TGG + tig0019143:117005-117024 Msa1370800:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG - tig0019143:117667-117686 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + tig0019143:117724-117743 Msa1370800:CDS 45.0%
GCAACTGCGTAAGCAAGATA+GGG - tig0019143:116403-116422 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG - tig0019143:116404-116423 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG + tig0019143:116462-116481 Msa1370800:CDS 45.0%
GAAGACCAACACGTACCTTT+GGG - tig0019143:116480-116499 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG + tig0019143:116560-116579 Msa1370800:CDS 45.0%
! CCCATTTAGGATCTTCTGCA+AGG - tig0019143:116564-116583 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG - tig0019143:116577-116596 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG - tig0019143:116606-116625 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG - tig0019143:116617-116636 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG - tig0019143:116670-116689 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG - tig0019143:116694-116713 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG - tig0019143:116695-116714 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG + tig0019143:116694-116713 Msa1370800:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG + tig0019143:116826-116845 Msa1370800:CDS 45.0%
GTTCACTTCTAGGTCCTGTT+TGG + tig0019143:117005-117024 Msa1370800:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG - tig0019143:117667-117686 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG + tig0019143:117724-117743 Msa1370800:CDS 45.0%
GGAAGACCAACACGTACCTT+TGG - tig0019143:116481-116500 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + tig0019143:116673-116692 Msa1370800:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - tig0019143:116680-116699 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG - tig0019143:116481-116500 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG + tig0019143:116673-116692 Msa1370800:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG - tig0019143:116680-116699 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG + tig0019143:116472-116491 Msa1370800:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + tig0019143:117798-117817 Msa1370800:three_prime_UTR 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG + tig0019143:116472-116491 Msa1370800:CDS 55.0%
GAGCTGCAACGGTTGATCCA+TGG + tig0019143:117798-117817 Msa1370800:three_prime_UTR 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + tig0019143:117787-117806 Msa1370800:three_prime_UTR 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG + tig0019143:117787-117806 Msa1370800:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0019143 gene 116340 118034 116340 ID=Msa1370800;Name=Msa1370800
tig0019143 mRNA 116340 118034 116340 ID=Msa1370800-mRNA-1;Parent=Msa1370800;Name=Msa1370800-mRNA-1;_AED=0.03;_eAED=0.03;_QI=102|1|1|1|1|1|3|281|170
tig0019143 exon 116340 116715 116340 ID=Msa1370800-mRNA-1:exon:1787;Parent=Msa1370800-mRNA-1
tig0019143 exon 116823 116944 116823 ID=Msa1370800-mRNA-1:exon:1788;Parent=Msa1370800-mRNA-1
tig0019143 exon 117637 118034 117637 ID=Msa1370800-mRNA-1:exon:1789;Parent=Msa1370800-mRNA-1
tig0019143 five_prime_UTR 116340 116441 116340 ID=Msa1370800-mRNA-1:five_prime_utr;Parent=Msa1370800-mRNA-1
tig0019143 CDS 116442 116715 116442 ID=Msa1370800-mRNA-1:cds;Parent=Msa1370800-mRNA-1
tig0019143 CDS 116823 116944 116823 ID=Msa1370800-mRNA-1:cds;Parent=Msa1370800-mRNA-1
tig0019143 CDS 117637 117753 117637 ID=Msa1370800-mRNA-1:cds;Parent=Msa1370800-mRNA-1
tig0019143 three_prime_UTR 117754 118034 117754 ID=Msa1370800-mRNA-1:three_prime_utr;Parent=Msa1370800-mRNA-1
Gene Sequence

>Msa1370800

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATAAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa1370800

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYKDKNPLIVGK*