AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0174830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174830 MtrunA17_Chr1g0195681 99.412 170 1 0 1 170 1 170 7.63e-123 343
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174830 AT1G02870.1 56.477 193 60 4 1 169 1 193 6.17e-68 205

Find 58 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATAGCTTTCTAAAGTTAAT+TGG 0.136445 1_4:-60596182 Msa0174830:three_prime_UTR
CGAGTATGAGATTAGGGTTT+AGG 0.234991 1_4:+60597703 None:intergenic
AAAGCGGGTTTCAGAACTTT+AGG 0.241847 1_4:+60597562 None:intergenic
CCCTTGCAGAAGATCCTAAA+TGG 0.241943 1_4:-60597513 Msa0174830:CDS
TTAATAGATGAAAGTAGTTT+TGG 0.244162 1_4:-60595990 Msa0174830:three_prime_UTR
GAGCCTTTATCGTCCCATTT+AGG 0.265744 1_4:+60597499 None:intergenic
TGAAATCGGCGTTGGGTAAA+AGG 0.283106 1_4:-60597247 Msa0174830:CDS
GCAAGGGATTGTAACAGTTT+TGG 0.284108 1_4:+60597529 None:intergenic
CGCCGAAAGAGGAATAGTTT+TGG 0.317209 1_4:+60597470 None:intergenic
AGAACTTTAGGGTTCTTCTT+TGG 0.324436 1_4:+60597574 None:intergenic
TTGGGAAAGATGTAGTATTC+TGG 0.325763 1_4:-60596040 Msa0174830:three_prime_UTR
GAGTATGAGATTAGGGTTTA+GGG 0.339426 1_4:+60597704 None:intergenic
ACCCAAAACTATTCCTCTTT+CGG 0.353542 1_4:-60597472 Msa0174830:CDS
GGAAGACCAACACGTACCTT+TGG 0.363935 1_4:+60597595 None:intergenic
TGCAACTGCGTAAGCAAGAT+AGG 0.370438 1_4:+60597674 None:intergenic
AGAGAATCAGTAAGGGAGTT+AGG 0.373552 1_4:+60597436 None:intergenic
CACAAAGAGAGAAGAAGAAT+GGG 0.408439 1_4:-60597649 Msa0174830:exon
GACAAGAATCCGCTCATTGT+TGG 0.413347 1_4:-60596278 Msa0174830:CDS
CTATGGACAAATTTACAATC+TGG 0.413457 1_4:-60596238 Msa0174830:three_prime_UTR
ATAGCTTTCTAAAGTTAATT+GGG 0.414342 1_4:-60596181 Msa0174830:three_prime_UTR
AAGCGGGTTTCAGAACTTTA+GGG 0.416206 1_4:+60597563 None:intergenic
GAAGACCAACACGTACCTTT+GGG 0.427653 1_4:+60597596 None:intergenic
GATCCTAAATGGGACGATAA+AGG 0.428418 1_4:-60597502 Msa0174830:CDS
ACACAAAGAGAGAAGAAGAA+TGG 0.467361 1_4:-60597650 Msa0174830:exon
TTGTGGTGTAGATTTGAAAT+CGG 0.469021 1_4:-60597261 Msa0174830:intron
GAATTTGAAGAGAATCAGTA+AGG 0.477015 1_4:+60597428 None:intergenic
GTCTTCTTCAAGATCACTGC+CGG 0.487579 1_4:+60597381 None:intergenic
GTAGATTTGAAATCGGCGTT+GGG 0.505626 1_4:-60597254 Msa0174830:intron
TCCATGCAAAATTCAAAAGC+AGG 0.510757 1_4:+60596069 None:intergenic
TATGGACAAATTTACAATCT+GGG 0.511461 1_4:-60596237 Msa0174830:three_prime_UTR
TGAATTGAAGTCAAAGGTTG+AGG 0.526816 1_4:+60597200 None:intergenic
TGTAGATTTGAAATCGGCGT+TGG 0.527237 1_4:-60597255 Msa0174830:intron
ACAAATTTACAATCTGGGCG+TGG 0.531570 1_4:-60596232 Msa0174830:three_prime_UTR
ATCAGAAACAACGCCGAAAG+AGG 0.531608 1_4:+60597459 None:intergenic
GCGTGGGTTGTGAGCTGCAA+CGG 0.531681 1_4:-60596215 Msa0174830:three_prime_UTR
GTTGCAACTGAATGCTGCAT+AGG 0.533068 1_4:+60596338 None:intergenic
CAGATTGTAAATTTGTCCAT+AGG 0.535480 1_4:+60596239 None:intergenic
TTGCAACTGAATGCTGCATA+GGG 0.539277 1_4:+60596339 None:intergenic
CCTTGCAGAAGATCCTAAAT+GGG 0.546570 1_4:-60597512 Msa0174830:CDS
CCATTTAGGATCTTCTGCAA+GGG 0.547386 1_4:+60597513 None:intergenic
TAACTTTAGAAAGCTATCCA+TGG 0.552988 1_4:+60596187 None:intergenic
CCCATTTAGGATCTTCTGCA+AGG 0.554925 1_4:+60597512 None:intergenic
CAAATTTACAATCTGGGCGT+GGG 0.557585 1_4:-60596231 Msa0174830:three_prime_UTR
GTTGCCGATGATAACGATCC+CGG 0.559862 1_4:-60597400 Msa0174830:CDS
TTTGGAGGAATAGTGAAAGC+GGG 0.577554 1_4:+60597547 None:intergenic
TCTTCTTCAAGATCACTGCC+GGG 0.597126 1_4:+60597382 None:intergenic
TCGTTATCATCGGCAACAGA+AGG 0.598477 1_4:+60597406 None:intergenic
GCAACTGCGTAAGCAAGATA+GGG 0.606004 1_4:+60597675 None:intergenic
AGACGCTGAATTGAAGTCAA+AGG 0.608242 1_4:+60597194 None:intergenic
GAGCTGCAACGGTTGATCCA+TGG 0.610724 1_4:-60596204 Msa0174830:three_prime_UTR
AATTTGAAGAGAATCAGTAA+GGG 0.613175 1_4:+60597429 None:intergenic
TTGGGTAAAAGGAGAAGAGA+TGG 0.616129 1_4:-60597236 Msa0174830:CDS
TTTCATTTACCAACAATGAG+CGG 0.625317 1_4:+60596269 None:intergenic
GGCAGTGATCTTGAAGAAGA+CGG 0.629513 1_4:-60597379 Msa0174830:intron
TCTCGCCCAAAGGTACGTGT+TGG 0.632503 1_4:-60597601 Msa0174830:CDS
GTATAAGCAATCTCGCCCAA+AGG 0.632811 1_4:-60597611 Msa0174830:CDS
ACTGCCGGGATCGTTATCAT+CGG 0.664050 1_4:+60597396 None:intergenic
AGCAAAATGTTGTTACAATG+AGG 0.667627 1_4:-60596151 Msa0174830:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_4:60597334-60597353 Msa0174830:intron 10.0%
!!! TTATTGTTTTGTTTTTATTT+TGG - chr1_4:60597334-60597353 Msa0174830:intron 10.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_4:60596925-60596944 None:intergenic 15.0%
!! AGTAAAATGTTTATGATTAA+GGG + chr1_4:60597114-60597133 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG + chr1_4:60597729-60597748 None:intergenic 15.0%
!! AACTAATTTATTTGCATAAA+CGG + chr1_4:60596925-60596944 None:intergenic 15.0%
!! AGTAAAATGTTTATGATTAA+GGG + chr1_4:60597114-60597133 None:intergenic 15.0%
!!! CTTTCATCTATTAATTTTTT+TGG + chr1_4:60597729-60597748 None:intergenic 15.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_4:60596405-60596424 Msa0174830:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_4:60596439-60596458 Msa0174830:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG + chr1_4:60596741-60596760 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG + chr1_4:60596855-60596874 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG + chr1_4:60596856-60596875 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG + chr1_4:60596972-60596991 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+AGG + chr1_4:60597115-60597134 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG - chr1_4:60597125-60597144 Msa0174830:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG - chr1_4:60597182-60597201 Msa0174830:CDS 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_4:60597544-60597563 Msa0174830:CDS 20.0%
!!! TTAATAGATGAAAGTAGTTT+TGG - chr1_4:60597735-60597754 Msa0174830:five_prime_UTR 20.0%
!! GTTGAAATTAGTATTAATCT+TGG - chr1_4:60596405-60596424 Msa0174830:intron 20.0%
!! GAATGTAAATAATTTGAGTA+TGG - chr1_4:60596439-60596458 Msa0174830:intron 20.0%
!!! TTTATCTTTTTCTATAGAAG+TGG + chr1_4:60596741-60596760 None:intergenic 20.0%
!!! TAAATTTTGTGAGATTGTTT+GGG + chr1_4:60596855-60596874 None:intergenic 20.0%
!!! ATAAATTTTGTGAGATTGTT+TGG + chr1_4:60596856-60596875 None:intergenic 20.0%
!!! TGTTTTTTCATAAATAACCT+TGG + chr1_4:60596972-60596991 None:intergenic 20.0%
!! GAGTAAAATGTTTATGATTA+AGG + chr1_4:60597115-60597134 None:intergenic 20.0%
!!! CATTTTACTCATATACTATA+TGG - chr1_4:60597125-60597144 Msa0174830:intron 20.0%
!! TATCATAATCTTGAAAACAT+CGG - chr1_4:60597182-60597201 Msa0174830:CDS 20.0%
!!! ATAGCTTTCTAAAGTTAATT+GGG - chr1_4:60597544-60597563 Msa0174830:CDS 20.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_4:60596299-60596318 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_4:60596378-60596397 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_4:60596417-60596436 Msa0174830:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_4:60596447-60596466 Msa0174830:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_4:60596561-60596580 Msa0174830:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_4:60596575-60596594 Msa0174830:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_4:60596674-60596693 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_4:60596980-60596999 Msa0174830:intron 25.0%
! TACTCATATACTATATGGTT+AGG - chr1_4:60597130-60597149 Msa0174830:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG - chr1_4:60597470-60597489 Msa0174830:CDS 25.0%
! TATGGACAAATTTACAATCT+GGG - chr1_4:60597488-60597507 Msa0174830:CDS 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_4:60597543-60597562 Msa0174830:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_4:60597666-60597685 Msa0174830:five_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_4:60597667-60597686 Msa0174830:five_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_4:60597714-60597733 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG + chr1_4:60597715-60597734 None:intergenic 25.0%
! AATTTGAAGAGAATCAGTAA+GGG + chr1_4:60596299-60596318 None:intergenic 25.0%
! TAACAATTTACGATGAATTG+AGG + chr1_4:60596378-60596397 None:intergenic 25.0%
!! ATTAATCTTGGTAATTCTGA+AGG - chr1_4:60596417-60596436 Msa0174830:intron 25.0%
!!! ATAATTTGAGTATGGTTTTG+TGG - chr1_4:60596447-60596466 Msa0174830:intron 25.0%
! AGTCGTTTAGTTGAAAAATA+TGG - chr1_4:60596561-60596580 Msa0174830:intron 25.0%
!! AAAATATGGAGATGATTTTC+AGG - chr1_4:60596575-60596594 Msa0174830:intron 25.0%
!!! GATAATTTGTTTATCCAAAC+AGG + chr1_4:60596674-60596693 None:intergenic 25.0%
! TATGAAAAAACAGCTTATGA+AGG - chr1_4:60596980-60596999 Msa0174830:intron 25.0%
! TACTCATATACTATATGGTT+AGG - chr1_4:60597130-60597149 Msa0174830:intron 25.0%
!! TAAATGAAATGTTTTGCCTA+TGG - chr1_4:60597470-60597489 Msa0174830:CDS 25.0%
! TATGGACAAATTTACAATCT+GGG - chr1_4:60597488-60597507 Msa0174830:CDS 25.0%
!!! GATAGCTTTCTAAAGTTAAT+TGG - chr1_4:60597543-60597562 Msa0174830:CDS 25.0%
!! AATTTTGCATGGATTTTTGT+TGG - chr1_4:60597666-60597685 Msa0174830:five_prime_UTR 25.0%
!! ATTTTGCATGGATTTTTGTT+GGG - chr1_4:60597667-60597686 Msa0174830:five_prime_UTR 25.0%
!!! TTTTTTGGCAACAACAATTT+GGG + chr1_4:60597714-60597733 None:intergenic 25.0%
!!! TTTTTTTGGCAACAACAATT+TGG + chr1_4:60597715-60597734 None:intergenic 25.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_4:60596031-60596050 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_4:60596300-60596319 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_4:60596464-60596483 Msa0174830:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG - chr1_4:60596861-60596880 Msa0174830:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_4:60596952-60596971 Msa0174830:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_4:60597221-60597240 Msa0174830:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_4:60597285-60597304 Msa0174830:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_4:60597292-60597311 Msa0174830:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_4:60597307-60597326 Msa0174830:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_4:60597350-60597369 Msa0174830:intron 30.0%
TTTCATTTACCAACAATGAG+CGG + chr1_4:60597459-60597478 None:intergenic 30.0%
CAGATTGTAAATTTGTCCAT+AGG + chr1_4:60597489-60597508 None:intergenic 30.0%
CTATGGACAAATTTACAATC+TGG - chr1_4:60597487-60597506 Msa0174830:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_4:60597541-60597560 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG - chr1_4:60597574-60597593 Msa0174830:CDS 30.0%
!!! GTTTTTCGAGTATGAGATTA+GGG + chr1_4:60596031-60596050 None:intergenic 30.0%
GAATTTGAAGAGAATCAGTA+AGG + chr1_4:60596300-60596319 None:intergenic 30.0%
! TTGTGGTGTAGATTTGAAAT+CGG - chr1_4:60596464-60596483 Msa0174830:intron 30.0%
TCTCACAAAATTTATGTCAG+TGG - chr1_4:60596861-60596880 Msa0174830:intron 30.0%
TATTATTCATAAGCTTGCCA+AGG - chr1_4:60596952-60596971 Msa0174830:intron 30.0%
! TCTAAAGTAACACAAATGAG+TGG - chr1_4:60597221-60597240 Msa0174830:CDS 30.0%
! CAAATGTTACTCTGTTTTCT+TGG - chr1_4:60597285-60597304 Msa0174830:intron 30.0%
!! TACTCTGTTTTCTTGGTTTT+AGG - chr1_4:60597292-60597311 Msa0174830:intron 30.0%
!!! GTTTTAGGACTTCATTTATG+TGG - chr1_4:60597307-60597326 Msa0174830:intron 30.0%
!! ATTTTGGCAGAAAATGATGA+TGG - chr1_4:60597350-60597369 Msa0174830:intron 30.0%
TTTCATTTACCAACAATGAG+CGG + chr1_4:60597459-60597478 None:intergenic 30.0%
CAGATTGTAAATTTGTCCAT+AGG + chr1_4:60597489-60597508 None:intergenic 30.0%
CTATGGACAAATTTACAATC+TGG - chr1_4:60597487-60597506 Msa0174830:CDS 30.0%
! TAACTTTAGAAAGCTATCCA+TGG + chr1_4:60597541-60597560 None:intergenic 30.0%
AGCAAAATGTTGTTACAATG+AGG - chr1_4:60597574-60597593 Msa0174830:CDS 30.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_4:60596024-60596043 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_4:60596032-60596051 None:intergenic 35.0%
ACACAAAGAGAGAAGAAGAA+TGG - chr1_4:60596075-60596094 Msa0174830:three_prime_UTR 35.0%
CACAAAGAGAGAAGAAGAAT+GGG - chr1_4:60596076-60596095 Msa0174830:three_prime_UTR 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_4:60596154-60596173 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_4:60596182-60596201 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_4:60596253-60596272 Msa0174830:exon 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_4:60596528-60596547 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_4:60596600-60596619 Msa0174830:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_4:60596647-60596666 Msa0174830:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG - chr1_4:60596899-60596918 Msa0174830:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG - chr1_4:60596997-60597016 Msa0174830:intron 35.0%
! GATTAAGGGCTCTTTTTGAT+TGG + chr1_4:60597100-60597119 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_4:60597655-60597674 Msa0174830:five_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_4:60597659-60597678 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG - chr1_4:60597685-60597704 Msa0174830:five_prime_UTR 35.0%
GAGTATGAGATTAGGGTTTA+GGG + chr1_4:60596024-60596043 None:intergenic 35.0%
!!! GGTTTTTCGAGTATGAGATT+AGG + chr1_4:60596032-60596051 None:intergenic 35.0%
ACACAAAGAGAGAAGAAGAA+TGG - chr1_4:60596075-60596094 Msa0174830:three_prime_UTR 35.0%
CACAAAGAGAGAAGAAGAAT+GGG - chr1_4:60596076-60596095 Msa0174830:three_prime_UTR 35.0%
!! AGAACTTTAGGGTTCTTCTT+TGG + chr1_4:60596154-60596173 None:intergenic 35.0%
!! TTTTGGAGGAATAGTGAAAG+CGG + chr1_4:60596182-60596201 None:intergenic 35.0%
ACCCAAAACTATTCCTCTTT+CGG - chr1_4:60596253-60596272 Msa0174830:exon 35.0%
TGAATTGAAGTCAAAGGTTG+AGG + chr1_4:60596528-60596547 None:intergenic 35.0%
!!! CGTTTTTTTCACTGTTGCTT+TGG - chr1_4:60596600-60596619 Msa0174830:intron 35.0%
! AGCTTTGATAGTTCACTTCT+AGG - chr1_4:60596647-60596666 Msa0174830:intron 35.0%
ACTGTCCCAATAAACCTTTA+GGG - chr1_4:60596899-60596918 Msa0174830:intron 35.0%
!! TGAAGGTGTACAATTTTCGT+CGG - chr1_4:60596997-60597016 Msa0174830:intron 35.0%
! GATTAAGGGCTCTTTTTGAT+TGG + chr1_4:60597100-60597119 None:intergenic 35.0%
!! TCCTGCTTTTGAATTTTGCA+TGG - chr1_4:60597655-60597674 Msa0174830:five_prime_UTR 35.0%
TCCATGCAAAATTCAAAAGC+AGG + chr1_4:60597659-60597678 None:intergenic 35.0%
TTGGGAAAGATGTAGTATTC+TGG - chr1_4:60597685-60597704 Msa0174830:five_prime_UTR 35.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_4:60596025-60596044 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_4:60596165-60596184 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_4:60596166-60596185 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_4:60596181-60596200 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_4:60596196-60596215 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_4:60596199-60596218 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_4:60596215-60596234 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_4:60596213-60596232 Msa0174830:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_4:60596223-60596242 Msa0174830:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_4:60596257-60596276 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_4:60596292-60596311 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_4:60596470-60596489 Msa0174830:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_4:60596471-60596490 Msa0174830:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_4:60596489-60596508 Msa0174830:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_4:60596534-60596553 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG - chr1_4:60596898-60596917 Msa0174830:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_4:60596907-60596926 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_4:60596908-60596927 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_4:60596916-60596935 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG - chr1_4:60596998-60597017 Msa0174830:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG - chr1_4:60596999-60597018 Msa0174830:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_4:60597237-60597256 Msa0174830:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_4:60597389-60597408 None:intergenic 40.0%
ACAAATTTACAATCTGGGCG+TGG - chr1_4:60597493-60597512 Msa0174830:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_4:60597494-60597513 Msa0174830:CDS 40.0%
CGAGTATGAGATTAGGGTTT+AGG + chr1_4:60596025-60596044 None:intergenic 40.0%
! AAGCGGGTTTCAGAACTTTA+GGG + chr1_4:60596165-60596184 None:intergenic 40.0%
! AAAGCGGGTTTCAGAACTTT+AGG + chr1_4:60596166-60596185 None:intergenic 40.0%
TTTGGAGGAATAGTGAAAGC+GGG + chr1_4:60596181-60596200 None:intergenic 40.0%
!! AGGGATTGTAACAGTTTTGG+AGG + chr1_4:60596196-60596215 None:intergenic 40.0%
! GCAAGGGATTGTAACAGTTT+TGG + chr1_4:60596199-60596218 None:intergenic 40.0%
! CCATTTAGGATCTTCTGCAA+GGG + chr1_4:60596215-60596234 None:intergenic 40.0%
CCTTGCAGAAGATCCTAAAT+GGG - chr1_4:60596213-60596232 Msa0174830:three_prime_UTR 40.0%
GATCCTAAATGGGACGATAA+AGG - chr1_4:60596223-60596242 Msa0174830:three_prime_UTR 40.0%
! GCCGAAAGAGGAATAGTTTT+GGG + chr1_4:60596257-60596276 None:intergenic 40.0%
AGAGAATCAGTAAGGGAGTT+AGG + chr1_4:60596292-60596311 None:intergenic 40.0%
! TGTAGATTTGAAATCGGCGT+TGG - chr1_4:60596470-60596489 Msa0174830:intron 40.0%
! GTAGATTTGAAATCGGCGTT+GGG - chr1_4:60596471-60596490 Msa0174830:intron 40.0%
TTGGGTAAAAGGAGAAGAGA+TGG - chr1_4:60596489-60596508 Msa0174830:intron 40.0%
AGACGCTGAATTGAAGTCAA+AGG + chr1_4:60596534-60596553 None:intergenic 40.0%
GACTGTCCCAATAAACCTTT+AGG - chr1_4:60596898-60596917 Msa0174830:intron 40.0%
AACGGCCCTAAAGGTTTATT+GGG + chr1_4:60596907-60596926 None:intergenic 40.0%
AAACGGCCCTAAAGGTTTAT+TGG + chr1_4:60596908-60596927 None:intergenic 40.0%
ATTTGCATAAACGGCCCTAA+AGG + chr1_4:60596916-60596935 None:intergenic 40.0%
!! GAAGGTGTACAATTTTCGTC+GGG - chr1_4:60596998-60597017 Msa0174830:intron 40.0%
! AAGGTGTACAATTTTCGTCG+GGG - chr1_4:60596999-60597018 Msa0174830:intron 40.0%
!! TGAGTGGTTGTGATTTGTTG+AGG - chr1_4:60597237-60597256 Msa0174830:CDS 40.0%
TTGCAACTGAATGCTGCATA+GGG + chr1_4:60597389-60597408 None:intergenic 40.0%
ACAAATTTACAATCTGGGCG+TGG - chr1_4:60597493-60597512 Msa0174830:CDS 40.0%
CAAATTTACAATCTGGGCGT+GGG - chr1_4:60597494-60597513 Msa0174830:CDS 40.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_4:60596053-60596072 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_4:60596054-60596073 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_4:60596114-60596133 Msa0174830:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_4:60596132-60596151 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_4:60596212-60596231 Msa0174830:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_4:60596216-60596235 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_4:60596229-60596248 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_4:60596258-60596277 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_4:60596269-60596288 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_4:60596322-60596341 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_4:60596346-60596365 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_4:60596347-60596366 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_4:60596346-60596365 Msa0174830:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_4:60596478-60596497 Msa0174830:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_4:60596657-60596676 Msa0174830:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_4:60597390-60597409 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_4:60597447-60597466 Msa0174830:CDS 45.0%
GCAACTGCGTAAGCAAGATA+GGG + chr1_4:60596053-60596072 None:intergenic 45.0%
TGCAACTGCGTAAGCAAGAT+AGG + chr1_4:60596054-60596073 None:intergenic 45.0%
GTATAAGCAATCTCGCCCAA+AGG - chr1_4:60596114-60596133 Msa0174830:three_prime_UTR 45.0%
GAAGACCAACACGTACCTTT+GGG + chr1_4:60596132-60596151 None:intergenic 45.0%
CCCTTGCAGAAGATCCTAAA+TGG - chr1_4:60596212-60596231 Msa0174830:three_prime_UTR 45.0%
! CCCATTTAGGATCTTCTGCA+AGG + chr1_4:60596216-60596235 None:intergenic 45.0%
GAGCCTTTATCGTCCCATTT+AGG + chr1_4:60596229-60596248 None:intergenic 45.0%
! CGCCGAAAGAGGAATAGTTT+TGG + chr1_4:60596258-60596277 None:intergenic 45.0%
ATCAGAAACAACGCCGAAAG+AGG + chr1_4:60596269-60596288 None:intergenic 45.0%
TCGTTATCATCGGCAACAGA+AGG + chr1_4:60596322-60596341 None:intergenic 45.0%
TCTTCTTCAAGATCACTGCC+GGG + chr1_4:60596346-60596365 None:intergenic 45.0%
GTCTTCTTCAAGATCACTGC+CGG + chr1_4:60596347-60596366 None:intergenic 45.0%
GGCAGTGATCTTGAAGAAGA+CGG - chr1_4:60596346-60596365 Msa0174830:CDS 45.0%
! TGAAATCGGCGTTGGGTAAA+AGG - chr1_4:60596478-60596497 Msa0174830:intron 45.0%
GTTCACTTCTAGGTCCTGTT+TGG - chr1_4:60596657-60596676 Msa0174830:intron 45.0%
GTTGCAACTGAATGCTGCAT+AGG + chr1_4:60597390-60597409 None:intergenic 45.0%
GACAAGAATCCGCTCATTGT+TGG - chr1_4:60597447-60597466 Msa0174830:CDS 45.0%
GGAAGACCAACACGTACCTT+TGG + chr1_4:60596133-60596152 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_4:60596325-60596344 Msa0174830:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_4:60596332-60596351 None:intergenic 50.0%
GGAAGACCAACACGTACCTT+TGG + chr1_4:60596133-60596152 None:intergenic 50.0%
! GTTGCCGATGATAACGATCC+CGG - chr1_4:60596325-60596344 Msa0174830:CDS 50.0%
ACTGCCGGGATCGTTATCAT+CGG + chr1_4:60596332-60596351 None:intergenic 50.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_4:60596124-60596143 Msa0174830:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_4:60597521-60597540 Msa0174830:CDS 55.0%
! TCTCGCCCAAAGGTACGTGT+TGG - chr1_4:60596124-60596143 Msa0174830:three_prime_UTR 55.0%
GAGCTGCAACGGTTGATCCA+TGG - chr1_4:60597521-60597540 Msa0174830:CDS 55.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_4:60597510-60597529 Msa0174830:CDS 60.0%
! GCGTGGGTTGTGAGCTGCAA+CGG - chr1_4:60597510-60597529 Msa0174830:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60595990 60597757 60595990 ID=Msa0174830;Name=Msa0174830
chr1_4 mRNA 60595990 60597757 60595990 ID=Msa0174830-mRNA-1;Parent=Msa0174830;Name=Msa0174830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=104|1|1|1|1|1|3|281|170
chr1_4 exon 60595990 60596387 60595990 ID=Msa0174830-mRNA-1:exon:19229;Parent=Msa0174830-mRNA-1
chr1_4 exon 60597151 60597272 60597151 ID=Msa0174830-mRNA-1:exon:19228;Parent=Msa0174830-mRNA-1
chr1_4 exon 60597380 60597757 60597380 ID=Msa0174830-mRNA-1:exon:19227;Parent=Msa0174830-mRNA-1
chr1_4 five_prime_UTR 60597654 60597757 60597654 ID=Msa0174830-mRNA-1:five_prime_utr;Parent=Msa0174830-mRNA-1
chr1_4 CDS 60597380 60597653 60597380 ID=Msa0174830-mRNA-1:cds;Parent=Msa0174830-mRNA-1
chr1_4 CDS 60597151 60597272 60597151 ID=Msa0174830-mRNA-1:cds;Parent=Msa0174830-mRNA-1
chr1_4 CDS 60596271 60596387 60596271 ID=Msa0174830-mRNA-1:cds;Parent=Msa0174830-mRNA-1
chr1_4 three_prime_UTR 60595990 60596270 60595990 ID=Msa0174830-mRNA-1:three_prime_utr;Parent=Msa0174830-mRNA-1
Gene Sequence

>Msa0174830

ATGGGAAGATCACGAAGAAAGTATAAGCAATCTCGCCCAAAGGTACGTGTTGGTCTTCCAAAGAAGAACCCTAAAGTTCTGAAACCCGCTTTCACTATTCCTCCAAAACTGTTACAATCCCTTGCAGAAGATCCTAAATGGGACGATAAAGGCTCTGTCACCCAAAACTATTCCTCTTTCGGCGTTGTTTCTGATCCTAACTCCCTTACTGATTCTCTTCAAATTCCTTCTGTTGCCGATGATAACGATCCCGGCAGTGATCTTGAAGAAGACGATTTGAAATCGGCGTTGGGTAAAAGGAGAAGAGATGGTAAAAGCTCACTTCCTCAACCTTTGACTTCAATTCAGCGTCTTCATATTAGTCGTTTAGTTGAAAAATATGGAGATGATTTTCAGAAAATGATGATGGATATAAAGCTAAACCCTATGCAGCATTCAGTTGCAACTTTAGAGAAATTGTGCTTCAGCTATTACTTATATAAAGACAAGAATCCGCTCATTGTTGGTAAATGA
Protein sequence

>Msa0174830

MGRSRRKYKQSRPKVRVGLPKKNPKVLKPAFTIPPKLLQSLAEDPKWDDKGSVTQNYSSFGVVSDPNSLTDSLQIPSVADDNDPGSDLEEDDLKSALGKRRRDGKSSLPQPLTSIQRLHISRLVEKYGDDFQKMMMDIKLNPMQHSVATLEKLCFSYYLYKDKNPLIVGK*