AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0083980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0083980 MtrunA17_Chr1g0198131 96.721 305 10 0 1 305 1 305 0.0 615
Msa0083980 MtrunA17_Chr1g0174241 53.254 169 71 2 142 304 161 327 4.98e-58 189
Msa0083980 MtrunA17_Chr3g0122741 49.007 151 73 2 153 303 2 148 1.13e-48 159
Msa0083980 MtrunA17_Chr1g0174221 47.945 146 73 2 159 303 91 234 1.37e-42 147
Msa0083980 MtrunA17_Chr4g0005721 35.294 153 83 5 165 302 77 228 6.08e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0083980 AT3G57810.2 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0083980 AT3G57810.4 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0083980 AT3G57810.3 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0083980 AT2G38025.2 32.075 159 86 4 165 302 53 210 4.69e-15 73.2
Msa0083980 AT2G38025.1 32.075 159 86 4 165 302 76 233 8.56e-15 72.8
Msa0083980 AT3G57810.1 39.362 94 52 2 123 211 121 214 2.68e-14 71.2

Find 130 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACTTGCCGACGAATTA+AGG 0.213414 1_2:+63144313 Msa0083980:CDS
ATCATGCGGTATAAGTCTTC+TGG 0.238749 1_2:+63145331 Msa0083980:three_prime_UTR
CTTAATAATAAAACCCGTTA+TGG 0.284416 1_2:+63143008 Msa0083980:five_prime_UTR
CTCCACCGCCGGTTGAGATT+TGG 0.298384 1_2:-63143245 None:intergenic
AACATAAGTCTGTTGAATTC+TGG 0.316175 1_2:-63144630 None:intergenic
TGGAATGGTGGCGGCGGGCG+GGG 0.325826 1_2:-63143206 None:intergenic
TGAGATTTGGAGGTGGTTGC+AGG 0.327466 1_2:-63143232 None:intergenic
GGTGGAATGGTGGCGGCGGG+CGG 0.330689 1_2:-63143208 None:intergenic
AATTCAACAGACTTATGTTT+GGG 0.341402 1_2:+63144634 Msa0083980:CDS
TCTCTCATGAAGACATATAT+TGG 0.349253 1_2:-63145123 None:intergenic
GTGGAATGGTGGCGGCGGGC+GGG 0.352247 1_2:-63143207 None:intergenic
GCGCCACTCGCTCTCGTCCT+TGG 0.358149 1_2:+63143115 Msa0083980:CDS
AGATGCAAGCTCCATGGATT+TGG 0.364917 1_2:+63145143 Msa0083980:CDS
AGGAGAGCCTGAGCTATTAA+TGG 0.369529 1_2:+63144658 Msa0083980:CDS
AGAGAAGCGTGGGTGTTATA+AGG 0.369781 1_2:-63142900 None:intergenic
ACTGCAAAACAAAAGTTCAT+TGG 0.380213 1_2:-63145452 None:intergenic
GAAAGGCGTGGTGGTGGTGG+TGG 0.384833 1_2:-63143149 None:intergenic
ACATGGTCACTACGAACTAT+TGG 0.387672 1_2:+63145236 Msa0083980:CDS
TTCCATTCCAAGAAAAGAAA+AGG 0.389727 1_2:+63142866 Msa0083980:five_prime_UTR
TGGAGTCGGCGGCGGCGCCT+CGG 0.390531 1_2:-63143484 None:intergenic
GAATTCAACAGACTTATGTT+TGG 0.396680 1_2:+63144633 Msa0083980:CDS
CTGCGCTTGCCTTGCCCCGC+CGG 0.407300 1_2:+63143443 Msa0083980:CDS
GCTACTGGCATTTGAGCTGC+AGG 0.412012 1_2:+63145414 Msa0083980:three_prime_UTR
CAAAAGTGGACATTGTTGTT+TGG 0.413224 1_2:+63142843 Msa0083980:five_prime_UTR
TCGATGAAATAGCACATTGA+TGG 0.414542 1_2:-63145214 None:intergenic
GGCGGTGGAATGGTGGCGGC+GGG 0.418826 1_2:-63143211 None:intergenic
GGAAGGTTTAGGTGAGTTGG+TGG 0.420220 1_2:+63145495 Msa0083980:three_prime_UTR
ATCTTTCACTTCACGTCCCT+CGG 0.423234 1_2:-63143529 None:intergenic
GAAGCATCACACCGCTGCTT+CGG 0.424353 1_2:-63143314 None:intergenic
TCCCTCTGTCGACTTTCATT+AGG 0.424371 1_2:-63144294 None:intergenic
GCACTGTGTATGTGAGTGTA+TGG 0.425283 1_2:-63143053 None:intergenic
CTCTCGTCCGGGAAAACAGC+TGG 0.428780 1_2:-63143504 None:intergenic
TTGGAGGTGGTTGCAGGCGG+TGG 0.429035 1_2:-63143226 None:intergenic
AATGGAAGGTTTAGGTGAGT+TGG 0.429406 1_2:+63145492 Msa0083980:three_prime_UTR
TGCTCGATTCTTGATACTCA+TGG 0.435244 1_2:+63145551 Msa0083980:three_prime_UTR
ATTACTCCTTGCGGCGGCGA+TGG 0.436846 1_2:+63143288 Msa0083980:CDS
GGAACTTGCCGACGAATTAA+GGG 0.438594 1_2:+63144314 Msa0083980:CDS
CGTCCCTCGGAACTCTCGTC+CGG 0.439061 1_2:-63143516 None:intergenic
TTATCCTACGCACCCATAAC+GGG 0.440177 1_2:-63143021 None:intergenic
GAGTTCATCGTCACGTTCAT+CGG 0.445917 1_2:-63143559 None:intergenic
AAGGATCGTGGAACGTTGCT+TGG 0.447140 1_2:+63143361 Msa0083980:CDS
TTCATCGTTCTGACTCAGCT+TGG 0.448002 1_2:+63143406 Msa0083980:CDS
TGCTATATTTACCAAATCCA+TGG 0.450384 1_2:-63145154 None:intergenic
TTAATAATAAAACCCGTTAT+GGG 0.452990 1_2:+63143009 Msa0083980:five_prime_UTR
TGTGTCTATAGGTTTATAGA+AGG 0.457572 1_2:+63144587 Msa0083980:intron
TCATGCGGTATAAGTCTTCT+GGG 0.458739 1_2:+63145332 Msa0083980:three_prime_UTR
GCGGTGGAGCTGCTTCGATA+TGG 0.467969 1_2:+63143259 Msa0083980:CDS
GACGAAAGGCGTGGTGGTGG+TGG 0.470943 1_2:-63143152 None:intergenic
AGGCGGTGGAATGGTGGCGG+CGG 0.476240 1_2:-63143212 None:intergenic
CACCGCCGGTTGAGATTTGG+AGG 0.479044 1_2:-63143242 None:intergenic
GTATCAACGTCAACAATAAC+CGG 0.479760 1_2:-63143462 None:intergenic
CTTGGGATGCTCGTCCTGCT+AGG 0.480235 1_2:+63143379 Msa0083980:CDS
TACTTTAACCCTTAATTCGT+CGG 0.484358 1_2:-63144322 None:intergenic
ATATGCAATCAATGTACAAA+AGG 0.492528 1_2:-63145719 None:intergenic
AAAGGCGTGGTGGTGGTGGT+GGG 0.493789 1_2:-63143148 None:intergenic
CTCCAAATCTCAACCGGCGG+TGG 0.496363 1_2:+63143243 Msa0083980:CDS
GGTGGTTGCAGGCGGTGGAA+TGG 0.499927 1_2:-63143221 None:intergenic
GCCACTCACTCATCCATGCT+TGG 0.503058 1_2:+63143084 Msa0083980:exon
ATCCTTCACCTTTGAGCTCG+TGG 0.505031 1_2:-63143344 None:intergenic
TTGGTAAATATAGCAAAATA+CGG 0.508333 1_2:+63145162 Msa0083980:CDS
AGAGAAGAGAGCAATAAGAA+TGG 0.511242 1_2:-63142949 None:intergenic
CGCCGGTTGAGATTTGGAGG+TGG 0.513387 1_2:-63143239 None:intergenic
CACTGTGTATGTGAGTGTAT+GGG 0.514314 1_2:-63143052 None:intergenic
TGAGAAGCCATTAATAGCTC+AGG 0.515628 1_2:-63144665 None:intergenic
AAAACAGCTGGAGTCGGCGG+CGG 0.517656 1_2:-63143492 None:intergenic
AACCACCTCCAAATCTCAAC+CGG 0.518771 1_2:+63143237 Msa0083980:CDS
GTTATCCTACGCACCCATAA+CGG 0.524419 1_2:-63143022 None:intergenic
AGGATCGTGGAACGTTGCTT+GGG 0.525778 1_2:+63143362 Msa0083980:CDS
CTACTGGCATTTGAGCTGCA+GGG 0.526098 1_2:+63145415 Msa0083980:three_prime_UTR
TGCAGGCGGTGGAATGGTGG+CGG 0.528482 1_2:-63143215 None:intergenic
CGGCGGGCGGGGAAAGTGAG+AGG 0.534229 1_2:-63143195 None:intergenic
GCTCGATTCTTGATACTCAT+GGG 0.538567 1_2:+63145552 Msa0083980:three_prime_UTR
GTCCCTCGGAACTCTCGTCC+GGG 0.539858 1_2:-63143515 None:intergenic
CTGTTCAGAGCAATAGCTCA+TGG 0.539965 1_2:+63144243 Msa0083980:CDS
CAAAAGGAGCCAACGAATGT+TGG 0.540663 1_2:-63145703 None:intergenic
TTAAGTGAACCAACATTCGT+TGG 0.542574 1_2:+63145694 Msa0083980:three_prime_UTR
GTGTCTATAGGTTTATAGAA+GGG 0.543445 1_2:+63144588 Msa0083980:intron
TGTCTATAGGTTTATAGAAG+GGG 0.544260 1_2:+63144589 Msa0083980:intron
GAACGATGAAGCCACCTAGC+AGG 0.545883 1_2:-63143393 None:intergenic
GGTTGCAGGCGGTGGAATGG+TGG 0.547561 1_2:-63143218 None:intergenic
CAACAATAACCGGCGGGGCA+AGG 0.548314 1_2:-63143452 None:intergenic
TAGGGGTGCTTGCTGATGGT+CGG 0.550798 1_2:+63144217 Msa0083980:intron
TCCATTCCAAGAAAAGAAAA+GGG 0.553262 1_2:+63142867 Msa0083980:five_prime_UTR
CGGAATCCATCGCCGCCGCA+AGG 0.559044 1_2:-63143294 None:intergenic
GGCGCAGAGTACACCAAGCA+TGG 0.563817 1_2:-63143097 None:intergenic
AGCTCAAAGGTGAAGGATCG+TGG 0.569548 1_2:+63143349 Msa0083980:CDS
AACGCTGATTACAGAGTCAC+GGG 0.570421 1_2:+63143582 Msa0083980:CDS
CATGAGAGATGCAAGCTCCA+TGG 0.570844 1_2:+63145137 Msa0083980:CDS
GAAGAGAAAGAGAGAAGCGT+GGG 0.573463 1_2:-63142910 None:intergenic
TCCGGGAAAACAGCTGGAGT+CGG 0.574081 1_2:-63143498 None:intergenic
CAACAGACTTATGTTTGGGG+AGG 0.576052 1_2:+63144638 Msa0083980:CDS
CAACGTCAACAATAACCGGC+GGG 0.576870 1_2:-63143458 None:intergenic
GACCACGAGCTCAAAGGTGA+AGG 0.577751 1_2:+63143342 Msa0083980:CDS
AAAACCCGTTATGGGTGCGT+AGG 0.577803 1_2:+63143017 Msa0083980:five_prime_UTR
GATCCAAGGACGAGAGCGAG+TGG 0.579496 1_2:-63143118 None:intergenic
GGTATAGGGGTGCTTGCTGA+TGG 0.584649 1_2:+63144213 Msa0083980:intron
TATCGAAGCAGCTCCACCGC+CGG 0.586713 1_2:-63143256 None:intergenic
CATCATGACCACGAGCTCAA+AGG 0.587256 1_2:+63143336 Msa0083980:CDS
TTCCCGGACGAGAGTTCCGA+GGG 0.588950 1_2:+63143513 Msa0083980:CDS
AGAGACGAAAGGCGTGGTGG+TGG 0.589008 1_2:-63143155 None:intergenic
AGTCTTAAAGAACACTTGCA+TGG 0.592866 1_2:+63145591 Msa0083980:three_prime_UTR
GTTTAGTTTCTTTAATCATG+CGG 0.593638 1_2:+63145317 Msa0083980:three_prime_UTR
GAAAGTGAGAGGAGCGACAG+TGG 0.606183 1_2:-63143184 None:intergenic
CAACGCTGATTACAGAGTCA+CGG 0.607136 1_2:+63143581 Msa0083980:CDS
CACGCGATTACTCCTTGCGG+CGG 0.611520 1_2:+63143282 Msa0083980:CDS
TTTCCCGGACGAGAGTTCCG+AGG 0.612131 1_2:+63143512 Msa0083980:CDS
GCCTAATGAAAGTCGACAGA+GGG 0.612158 1_2:+63144293 Msa0083980:CDS
CGAAGAGAAAGAGAGAAGCG+TGG 0.621048 1_2:-63142911 None:intergenic
GGCGATGGATTCCGAAGCAG+CGG 0.621773 1_2:+63143303 Msa0083980:CDS
TCCAAGAAAAGAAAAGGGTG+AGG 0.622110 1_2:+63142872 Msa0083980:five_prime_UTR
TATTGTTGACGTTGATACCG+AGG 0.628764 1_2:+63143467 Msa0083980:CDS
GGGAAAACAGCTGGAGTCGG+CGG 0.628808 1_2:-63143495 None:intergenic
TGAAGAATATATGAATGATG+AGG 0.630448 1_2:+63145185 Msa0083980:CDS
ACCAAGCATGGATGAGTGAG+TGG 0.631102 1_2:-63143085 None:intergenic
GATTTGGAGGTGGTTGCAGG+CGG 0.637060 1_2:-63143229 None:intergenic
ACTGTGTATGTGAGTGTATG+GGG 0.640581 1_2:-63143051 None:intergenic
TGGTGGGAAGAGAAGATCCA+AGG 0.650314 1_2:-63143132 None:intergenic
CGCCTAATGAAAGTCGACAG+AGG 0.650904 1_2:+63144292 Msa0083980:CDS
CACCTCCAAATCTCAACCGG+CGG 0.653859 1_2:+63143240 Msa0083980:CDS
GGGATGCTCGTCCTGCTAGG+TGG 0.655002 1_2:+63143382 Msa0083980:CDS
ACAGGCGCAAGGAAACAGAA+TGG 0.661069 1_2:+63144476 Msa0083980:CDS
ATTCAACAGACTTATGTTTG+GGG 0.663997 1_2:+63144635 Msa0083980:CDS
AATGTGCTATTTCATCGACA+TGG 0.668256 1_2:+63145219 Msa0083980:CDS
GAAAAGAAAAGGGTGAGGTG+AGG 0.669407 1_2:+63142877 Msa0083980:five_prime_UTR
AACGTCAACAATAACCGGCG+GGG 0.672807 1_2:-63143457 None:intergenic
GAGAGAGACGAAAGGCGTGG+TGG 0.678901 1_2:-63143158 None:intergenic
CGTGAGAGAGACGAAAGGCG+TGG 0.686067 1_2:-63143161 None:intergenic
AGTGGCGTGAGAGAGACGAA+AGG 0.697149 1_2:-63143166 None:intergenic
TCAACGTCAACAATAACCGG+CGG 0.700101 1_2:-63143459 None:intergenic
TGGCACGCGATTACTCCTTG+CGG 0.725561 1_2:+63143279 Msa0083980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTACTTTTATTTACA+TGG + chr1_2:63144543-63144562 Msa0083980:intron 10.0%
!!! TTTTTGTATTTTCTTTTTAA+TGG + chr1_2:63145474-63145493 Msa0083980:three_prime_UTR 10.0%
!!! ATTTCATTTTTTGTCTTTAA+TGG + chr1_2:63143762-63143781 Msa0083980:intron 15.0%
!!! TATTTTTTTCATATAAGTAC+AGG + chr1_2:63143786-63143805 Msa0083980:intron 15.0%
!!! ATTTTTTTCATATAAGTACA+GGG + chr1_2:63143787-63143806 Msa0083980:intron 15.0%
!! AATAAAACATCACAAAAATT+GGG - chr1_2:63144434-63144453 None:intergenic 15.0%
!! AAATAAAACATCACAAAAAT+TGG - chr1_2:63144435-63144454 None:intergenic 15.0%
!!! ATTTTTGTGATGTTTTATTT+AGG + chr1_2:63144435-63144454 Msa0083980:intron 15.0%
!!! TTTTTATTTTTTTTGAAAGG+AGG + chr1_2:63145523-63145542 Msa0083980:three_prime_UTR 15.0%
!! TTAATAATAAAACCCGTTAT+GGG + chr1_2:63143009-63143028 Msa0083980:five_prime_UTR 20.0%
!!! TTATTGTTCAGTTATTTTGA+TGG + chr1_2:63144121-63144140 Msa0083980:intron 20.0%
!! TTGGTAAATATAGCAAAATA+CGG + chr1_2:63145162-63145181 Msa0083980:CDS 20.0%
!!! TGTATTTTCTTTTTAATGGA+AGG + chr1_2:63145478-63145497 Msa0083980:three_prime_UTR 20.0%
!!! TTCTCTTCGTTCTTTTTTAT+TGG + chr1_2:63142925-63142944 Msa0083980:five_prime_UTR 25.0%
! CTTAATAATAAAACCCGTTA+TGG + chr1_2:63143008-63143027 Msa0083980:five_prime_UTR 25.0%
! AACAAGAACTATTGTATCAT+TGG - chr1_2:63144035-63144054 None:intergenic 25.0%
! TATAAGTTTGTATCACTGAA+TGG + chr1_2:63144192-63144211 Msa0083980:intron 25.0%
! GGAAACTACATACAATTATT+TGG + chr1_2:63144517-63144536 Msa0083980:intron 25.0%
! AATTCAACAGACTTATGTTT+GGG + chr1_2:63144634-63144653 Msa0083980:CDS 25.0%
! TTTCAGTTACATTTGATTGA+TGG + chr1_2:63144742-63144761 Msa0083980:intron 25.0%
! TAATGAAAGATGATAATCGA+TGG + chr1_2:63144910-63144929 Msa0083980:intron 25.0%
! AATCGATGGATATTAATCTA+CGG + chr1_2:63144924-63144943 Msa0083980:intron 25.0%
! CTTATAGAGAAACAAATTTC+CGG + chr1_2:63144981-63145000 Msa0083980:intron 25.0%
! TAAGTTATGAAGACAATGTT+AGG - chr1_2:63145095-63145114 None:intergenic 25.0%
! TGAAGAATATATGAATGATG+AGG + chr1_2:63145185-63145204 Msa0083980:CDS 25.0%
!!! GTTATTTTTGAGACAACTTT+TGG - chr1_2:63145273-63145292 None:intergenic 25.0%
!! GTTTAGTTTCTTTAATCATG+CGG + chr1_2:63145317-63145336 Msa0083980:three_prime_UTR 25.0%
!!! TTTAGTAGTTTTTTTGCTAC+TGG + chr1_2:63145399-63145418 Msa0083980:three_prime_UTR 25.0%
!! TTCTTTTTAATGGAAGGTTT+AGG + chr1_2:63145484-63145503 Msa0083980:three_prime_UTR 25.0%
! ATATGCAATCAATGTACAAA+AGG - chr1_2:63145722-63145741 None:intergenic 25.0%
TTCCATTCCAAGAAAAGAAA+AGG + chr1_2:63142866-63142885 Msa0083980:five_prime_UTR 30.0%
TCCATTCCAAGAAAAGAAAA+GGG + chr1_2:63142867-63142886 Msa0083980:five_prime_UTR 30.0%
!!! ACGGGTTTTATTATTAAGAC+TGG - chr1_2:63143006-63143025 None:intergenic 30.0%
TTTCATATAAGTACAGGGAT+TGG + chr1_2:63143792-63143811 Msa0083980:intron 30.0%
TTACAACTACGAAACAACAA+CGG + chr1_2:63143950-63143969 Msa0083980:intron 30.0%
ACTTGTTCAATATGAAGTGT+CGG + chr1_2:63144072-63144091 Msa0083980:intron 30.0%
AACATGAGTTAGAGATTATG+TGG + chr1_2:63144162-63144181 Msa0083980:intron 30.0%
ACATGAGTTAGAGATTATGT+GGG + chr1_2:63144163-63144182 Msa0083980:intron 30.0%
TTTGTATCACTGAATGGTAT+AGG + chr1_2:63144198-63144217 Msa0083980:intron 30.0%
TTGTATCACTGAATGGTATA+GGG + chr1_2:63144199-63144218 Msa0083980:intron 30.0%
TACTTTAACCCTTAATTCGT+CGG - chr1_2:63144325-63144344 None:intergenic 30.0%
!! TCTTGCTAGAACTTTTGAAA+CGG + chr1_2:63144351-63144370 Msa0083980:intron 30.0%
AAGAATGTTGACAAAGAAGA+AGG - chr1_2:63144386-63144405 None:intergenic 30.0%
TGGGTAACAAAATTCATTGA+AGG - chr1_2:63144415-63144434 None:intergenic 30.0%
!! GTTATATTTGCTGTGTCTAT+AGG + chr1_2:63144576-63144595 Msa0083980:intron 30.0%
TGTGTCTATAGGTTTATAGA+AGG + chr1_2:63144587-63144606 Msa0083980:intron 30.0%
GTGTCTATAGGTTTATAGAA+GGG + chr1_2:63144588-63144607 Msa0083980:intron 30.0%
TGTCTATAGGTTTATAGAAG+GGG + chr1_2:63144589-63144608 Msa0083980:intron 30.0%
AACATAAGTCTGTTGAATTC+TGG - chr1_2:63144633-63144652 None:intergenic 30.0%
GAATTCAACAGACTTATGTT+TGG + chr1_2:63144633-63144652 Msa0083980:CDS 30.0%
ATTCAACAGACTTATGTTTG+GGG + chr1_2:63144635-63144654 Msa0083980:CDS 30.0%
AACATCATATCCTACAACAA+CGG + chr1_2:63144843-63144862 Msa0083980:intron 30.0%
ATCGATGGATATTAATCTAC+GGG + chr1_2:63144925-63144944 Msa0083980:intron 30.0%
! TTCATTTTGTGGTAAATCGT+TGG + chr1_2:63145004-63145023 Msa0083980:intron 30.0%
! TCATTTTGTGGTAAATCGTT+GGG + chr1_2:63145005-63145024 Msa0083980:intron 30.0%
CATTGTCTTCATAACTTACA+AGG + chr1_2:63145097-63145116 Msa0083980:intron 30.0%
TCTCTCATGAAGACATATAT+TGG - chr1_2:63145126-63145145 None:intergenic 30.0%
TGCTATATTTACCAAATCCA+TGG - chr1_2:63145157-63145176 None:intergenic 30.0%
!!! GAAGAGCTTTTTTTGTCATT+TGG + chr1_2:63145359-63145378 Msa0083980:three_prime_UTR 30.0%
ACTGCAAAACAAAAGTTCAT+TGG - chr1_2:63145455-63145474 None:intergenic 30.0%
! CAAAAGTGGACATTGTTGTT+TGG + chr1_2:63142843-63142862 Msa0083980:five_prime_UTR 35.0%
! ACCCTTTTCTTTTCTTGGAA+TGG - chr1_2:63142871-63142890 None:intergenic 35.0%
AGAGAAGAGAGCAATAAGAA+TGG - chr1_2:63142952-63142971 None:intergenic 35.0%
GTATCAACGTCAACAATAAC+CGG - chr1_2:63143465-63143484 None:intergenic 35.0%
! ATATAAGTACAGGGATTGGT+TGG + chr1_2:63143796-63143815 Msa0083980:intron 35.0%
ATTGAACAAGTGTCTTGAGT+TGG - chr1_2:63144063-63144082 None:intergenic 35.0%
TGTATCACTGAATGGTATAG+GGG + chr1_2:63144200-63144219 Msa0083980:intron 35.0%
! TTGCTGAAGAACTTTTGAAC+AGG + chr1_2:63144458-63144477 Msa0083980:CDS 35.0%
ACAAGTTAAACCGTTGTTGT+AGG - chr1_2:63144856-63144875 None:intergenic 35.0%
!! AACAACGGTTTAACTTGTTG+TGG + chr1_2:63144858-63144877 Msa0083980:intron 35.0%
! CAAATTTCCGGTTCATTTTG+TGG + chr1_2:63144993-63145012 Msa0083980:intron 35.0%
CGATTTACCACAAAATGAAC+CGG - chr1_2:63145003-63145022 None:intergenic 35.0%
GAAGACAATGTTAGGTACAA+CGG - chr1_2:63145087-63145106 None:intergenic 35.0%
TCGATGAAATAGCACATTGA+TGG - chr1_2:63145217-63145236 None:intergenic 35.0%
!! AATGTGCTATTTCATCGACA+TGG + chr1_2:63145219-63145238 Msa0083980:CDS 35.0%
! TGAGACAACTTTTGGTCATA+AGG - chr1_2:63145265-63145284 None:intergenic 35.0%
AGTCTTAAAGAACACTTGCA+TGG + chr1_2:63145591-63145610 Msa0083980:three_prime_UTR 35.0%
TGTCAGCATAAAAACTTCGT+TGG - chr1_2:63145635-63145654 None:intergenic 35.0%
TTAAGTGAACCAACATTCGT+TGG + chr1_2:63145694-63145713 Msa0083980:three_prime_UTR 35.0%
TCCAAGAAAAGAAAAGGGTG+AGG + chr1_2:63142872-63142891 Msa0083980:five_prime_UTR 40.0%
! ACCTCACCCTTTTCTTTTCT+TGG - chr1_2:63142876-63142895 None:intergenic 40.0%
ACTGTGTATGTGAGTGTATG+GGG - chr1_2:63143054-63143073 None:intergenic 40.0%
CACTGTGTATGTGAGTGTAT+GGG - chr1_2:63143055-63143074 None:intergenic 40.0%
TATTGTTGACGTTGATACCG+AGG + chr1_2:63143467-63143486 Msa0083980:CDS 40.0%
GTTTGTGACCAAGTGTTTGA+TGG + chr1_2:63143910-63143929 Msa0083980:intron 40.0%
GTGACAAACCATCAAACACT+TGG - chr1_2:63143921-63143940 None:intergenic 40.0%
! CATGGAGCTTGCTTGAAAAA+TGG + chr1_2:63144261-63144280 Msa0083980:CDS 40.0%
! AGCTTGCTTGAAAAATGGAG+AGG + chr1_2:63144266-63144285 Msa0083980:CDS 40.0%
!!! CTAGAACTTTTGAAACGGAG+AGG + chr1_2:63144356-63144375 Msa0083980:intron 40.0%
! TGGTGAGCTTTGTCATTTGA+AGG + chr1_2:63144496-63144515 Msa0083980:intron 40.0%
TGAGAAGCCATTAATAGCTC+AGG - chr1_2:63144668-63144687 None:intergenic 40.0%
CAATGTTAGGTACAACGGAA+TGG - chr1_2:63145082-63145101 None:intergenic 40.0%
ACATGGTCACTACGAACTAT+TGG + chr1_2:63145236-63145255 Msa0083980:CDS 40.0%
ATCATGCGGTATAAGTCTTC+TGG + chr1_2:63145331-63145350 Msa0083980:three_prime_UTR 40.0%
TCATGCGGTATAAGTCTTCT+GGG + chr1_2:63145332-63145351 Msa0083980:three_prime_UTR 40.0%
AATGGAAGGTTTAGGTGAGT+TGG + chr1_2:63145492-63145511 Msa0083980:three_prime_UTR 40.0%
TGCTCGATTCTTGATACTCA+TGG + chr1_2:63145551-63145570 Msa0083980:three_prime_UTR 40.0%
GCTCGATTCTTGATACTCAT+GGG + chr1_2:63145552-63145571 Msa0083980:three_prime_UTR 40.0%
GAAAAGAAAAGGGTGAGGTG+AGG + chr1_2:63142877-63142896 Msa0083980:five_prime_UTR 45.0%
! AGAGAAGCGTGGGTGTTATA+AGG - chr1_2:63142903-63142922 None:intergenic 45.0%
! GAAGAGAAAGAGAGAAGCGT+GGG - chr1_2:63142913-63142932 None:intergenic 45.0%
TTATCCTACGCACCCATAAC+GGG - chr1_2:63143024-63143043 None:intergenic 45.0%
GTTATCCTACGCACCCATAA+CGG - chr1_2:63143025-63143044 None:intergenic 45.0%
GCACTGTGTATGTGAGTGTA+TGG - chr1_2:63143056-63143075 None:intergenic 45.0%
AACCACCTCCAAATCTCAAC+CGG + chr1_2:63143237-63143256 Msa0083980:CDS 45.0%
TTCATCGTTCTGACTCAGCT+TGG + chr1_2:63143406-63143425 Msa0083980:CDS 45.0%
! GACTCAGCTTGGCTTCTTTT+CGG + chr1_2:63143417-63143436 Msa0083980:CDS 45.0%
TCAACGTCAACAATAACCGG+CGG - chr1_2:63143462-63143481 None:intergenic 45.0%
ATCTTTCACTTCACGTCCCT+CGG - chr1_2:63143532-63143551 None:intergenic 45.0%
GAGTTCATCGTCACGTTCAT+CGG - chr1_2:63143562-63143581 None:intergenic 45.0%
! CAACGCTGATTACAGAGTCA+CGG + chr1_2:63143581-63143600 Msa0083980:CDS 45.0%
! AACGCTGATTACAGAGTCAC+GGG + chr1_2:63143582-63143601 Msa0083980:CDS 45.0%
!! TAAGTACAGGGATTGGTTGG+TGG + chr1_2:63143799-63143818 Msa0083980:intron 45.0%
CTGTTCAGAGCAATAGCTCA+TGG + chr1_2:63144243-63144262 Msa0083980:CDS 45.0%
GCCTAATGAAAGTCGACAGA+GGG + chr1_2:63144293-63144312 Msa0083980:CDS 45.0%
!! TCCCTCTGTCGACTTTCATT+AGG - chr1_2:63144297-63144316 None:intergenic 45.0%
GGAACTTGCCGACGAATTAA+GGG + chr1_2:63144314-63144333 Msa0083980:CDS 45.0%
! AGAACTTTTGAACAGGCGCA+AGG + chr1_2:63144465-63144484 Msa0083980:CDS 45.0%
CAACAGACTTATGTTTGGGG+AGG + chr1_2:63144638-63144657 Msa0083980:CDS 45.0%
AGGAGAGCCTGAGCTATTAA+TGG + chr1_2:63144658-63144677 Msa0083980:CDS 45.0%
TGTGGTAAATCGTTGGGACT+TGG + chr1_2:63145011-63145030 Msa0083980:intron 45.0%
GTGGTAAATCGTTGGGACTT+GGG + chr1_2:63145012-63145031 Msa0083980:intron 45.0%
AGATGCAAGCTCCATGGATT+TGG + chr1_2:63145143-63145162 Msa0083980:CDS 45.0%
CAAAAGGAGCCAACGAATGT+TGG - chr1_2:63145706-63145725 None:intergenic 45.0%
!!! TTTTTTTTATTTTTTTTGAA+AGG + chr1_2:63145520-63145539 Msa0083980:three_prime_UTR 5.0%
CGAAGAGAAAGAGAGAAGCG+TGG - chr1_2:63142914-63142933 None:intergenic 50.0%
AAAACCCGTTATGGGTGCGT+AGG + chr1_2:63143017-63143036 Msa0083980:five_prime_UTR 50.0%
ACCAAGCATGGATGAGTGAG+TGG - chr1_2:63143088-63143107 None:intergenic 50.0%
TGGTGGGAAGAGAAGATCCA+AGG - chr1_2:63143135-63143154 None:intergenic 50.0%
TGAGATTTGGAGGTGGTTGC+AGG - chr1_2:63143235-63143254 None:intergenic 50.0%
CATCATGACCACGAGCTCAA+AGG + chr1_2:63143336-63143355 Msa0083980:CDS 50.0%
ATCCTTCACCTTTGAGCTCG+TGG - chr1_2:63143347-63143366 None:intergenic 50.0%
AGCTCAAAGGTGAAGGATCG+TGG + chr1_2:63143349-63143368 Msa0083980:CDS 50.0%
AAGGATCGTGGAACGTTGCT+TGG + chr1_2:63143361-63143380 Msa0083980:CDS 50.0%
AGGATCGTGGAACGTTGCTT+GGG + chr1_2:63143362-63143381 Msa0083980:CDS 50.0%
AACGTCAACAATAACCGGCG+GGG - chr1_2:63143460-63143479 None:intergenic 50.0%
CAACGTCAACAATAACCGGC+GGG - chr1_2:63143461-63143480 None:intergenic 50.0%
CGCCTAATGAAAGTCGACAG+AGG + chr1_2:63144292-63144311 Msa0083980:CDS 50.0%
GGGAACTTGCCGACGAATTA+AGG + chr1_2:63144313-63144332 Msa0083980:CDS 50.0%
ACAGGCGCAAGGAAACAGAA+TGG + chr1_2:63144476-63144495 Msa0083980:CDS 50.0%
CATGAGAGATGCAAGCTCCA+TGG + chr1_2:63145137-63145156 Msa0083980:CDS 50.0%
CTACTGGCATTTGAGCTGCA+GGG + chr1_2:63145415-63145434 Msa0083980:three_prime_UTR 50.0%
! GGAAGGTTTAGGTGAGTTGG+TGG + chr1_2:63145495-63145514 Msa0083980:three_prime_UTR 50.0%
GCCACTCACTCATCCATGCT+TGG + chr1_2:63143084-63143103 Msa0083980:exon 55.0%
AGTGGCGTGAGAGAGACGAA+AGG - chr1_2:63143169-63143188 None:intergenic 55.0%
GAAAGTGAGAGGAGCGACAG+TGG - chr1_2:63143187-63143206 None:intergenic 55.0%
GATTTGGAGGTGGTTGCAGG+CGG - chr1_2:63143232-63143251 None:intergenic 55.0%
CACCTCCAAATCTCAACCGG+CGG + chr1_2:63143240-63143259 Msa0083980:CDS 55.0%
TGGCACGCGATTACTCCTTG+CGG + chr1_2:63143279-63143298 Msa0083980:CDS 55.0%
! GAAGCATCACACCGCTGCTT+CGG - chr1_2:63143317-63143336 None:intergenic 55.0%
GACCACGAGCTCAAAGGTGA+AGG + chr1_2:63143342-63143361 Msa0083980:CDS 55.0%
GAACGATGAAGCCACCTAGC+AGG - chr1_2:63143396-63143415 None:intergenic 55.0%
TCCGGGAAAACAGCTGGAGT+CGG - chr1_2:63143501-63143520 None:intergenic 55.0%
!! GTACAGGGATTGGTTGGTGG+TGG + chr1_2:63143802-63143821 Msa0083980:intron 55.0%
!! TGGTTGGTGGTGGTGGTGTT+TGG + chr1_2:63143812-63143831 Msa0083980:intron 55.0%
!! GGTATAGGGGTGCTTGCTGA+TGG + chr1_2:63144213-63144232 Msa0083980:intron 55.0%
!! TAGGGGTGCTTGCTGATGGT+CGG + chr1_2:63144217-63144236 Msa0083980:intron 55.0%
GCTACTGGCATTTGAGCTGC+AGG + chr1_2:63145414-63145433 Msa0083980:three_prime_UTR 55.0%
GGCGCAGAGTACACCAAGCA+TGG - chr1_2:63143100-63143119 None:intergenic 60.0%
GATCCAAGGACGAGAGCGAG+TGG - chr1_2:63143121-63143140 None:intergenic 60.0%
!! AAAGGCGTGGTGGTGGTGGT+GGG - chr1_2:63143151-63143170 None:intergenic 60.0%
! AGAGACGAAAGGCGTGGTGG+TGG - chr1_2:63143158-63143177 None:intergenic 60.0%
! GAGAGAGACGAAAGGCGTGG+TGG - chr1_2:63143161-63143180 None:intergenic 60.0%
CGTGAGAGAGACGAAAGGCG+TGG - chr1_2:63143164-63143183 None:intergenic 60.0%
! CGCCGGTTGAGATTTGGAGG+TGG - chr1_2:63143242-63143261 None:intergenic 60.0%
! CACCGCCGGTTGAGATTTGG+AGG - chr1_2:63143245-63143264 None:intergenic 60.0%
CTCCAAATCTCAACCGGCGG+TGG + chr1_2:63143243-63143262 Msa0083980:CDS 60.0%
! CTCCACCGCCGGTTGAGATT+TGG - chr1_2:63143248-63143267 None:intergenic 60.0%
TATCGAAGCAGCTCCACCGC+CGG - chr1_2:63143259-63143278 None:intergenic 60.0%
GCGGTGGAGCTGCTTCGATA+TGG + chr1_2:63143259-63143278 Msa0083980:CDS 60.0%
CACGCGATTACTCCTTGCGG+CGG + chr1_2:63143282-63143301 Msa0083980:CDS 60.0%
ATTACTCCTTGCGGCGGCGA+TGG + chr1_2:63143288-63143307 Msa0083980:CDS 60.0%
! GGCGATGGATTCCGAAGCAG+CGG + chr1_2:63143303-63143322 Msa0083980:CDS 60.0%
CTTGGGATGCTCGTCCTGCT+AGG + chr1_2:63143379-63143398 Msa0083980:CDS 60.0%
CAACAATAACCGGCGGGGCA+AGG - chr1_2:63143455-63143474 None:intergenic 60.0%
AAAACAGCTGGAGTCGGCGG+CGG - chr1_2:63143495-63143514 None:intergenic 60.0%
GGGAAAACAGCTGGAGTCGG+CGG - chr1_2:63143498-63143517 None:intergenic 60.0%
!! GCCGACTCCAGCTGTTTTCC+CGG + chr1_2:63143497-63143516 Msa0083980:CDS 60.0%
CTCTCGTCCGGGAAAACAGC+TGG - chr1_2:63143507-63143526 None:intergenic 60.0%
TTTCCCGGACGAGAGTTCCG+AGG + chr1_2:63143512-63143531 Msa0083980:CDS 60.0%
TTCCCGGACGAGAGTTCCGA+GGG + chr1_2:63143513-63143532 Msa0083980:CDS 60.0%
!! CAGGGATTGGTTGGTGGTGG+TGG + chr1_2:63143805-63143824 Msa0083980:intron 60.0%
!! GAAAGGCGTGGTGGTGGTGG+TGG - chr1_2:63143152-63143171 None:intergenic 65.0%
!! GACGAAAGGCGTGGTGGTGG+TGG - chr1_2:63143155-63143174 None:intergenic 65.0%
TGCAGGCGGTGGAATGGTGG+CGG - chr1_2:63143218-63143237 None:intergenic 65.0%
GGTTGCAGGCGGTGGAATGG+TGG - chr1_2:63143221-63143240 None:intergenic 65.0%
GGTGGTTGCAGGCGGTGGAA+TGG - chr1_2:63143224-63143243 None:intergenic 65.0%
TTGGAGGTGGTTGCAGGCGG+TGG - chr1_2:63143229-63143248 None:intergenic 65.0%
GGGATGCTCGTCCTGCTAGG+TGG + chr1_2:63143382-63143401 Msa0083980:CDS 65.0%
GTCCCTCGGAACTCTCGTCC+GGG - chr1_2:63143518-63143537 None:intergenic 65.0%
CGTCCCTCGGAACTCTCGTC+CGG - chr1_2:63143519-63143538 None:intergenic 65.0%
GCGCCACTCGCTCTCGTCCT+TGG + chr1_2:63143115-63143134 Msa0083980:CDS 70.0%
!! AGGCGGTGGAATGGTGGCGG+CGG - chr1_2:63143215-63143234 None:intergenic 70.0%
CGGAATCCATCGCCGCCGCA+AGG - chr1_2:63143297-63143316 None:intergenic 70.0%
CGGCGGGCGGGGAAAGTGAG+AGG - chr1_2:63143198-63143217 None:intergenic 75.0%
!! TGGAATGGTGGCGGCGGGCG+GGG - chr1_2:63143209-63143228 None:intergenic 75.0%
!! GTGGAATGGTGGCGGCGGGC+GGG - chr1_2:63143210-63143229 None:intergenic 75.0%
!! GGTGGAATGGTGGCGGCGGG+CGG - chr1_2:63143211-63143230 None:intergenic 75.0%
!! GGCGGTGGAATGGTGGCGGC+GGG - chr1_2:63143214-63143233 None:intergenic 75.0%
CTGCGCTTGCCTTGCCCCGC+CGG + chr1_2:63143443-63143462 Msa0083980:CDS 75.0%
! TGGAGTCGGCGGCGGCGCCT+CGG - chr1_2:63143487-63143506 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 63142842 63145773 63142842 ID=Msa0083980;Name=Msa0083980
chr1_2 mRNA 63142842 63145773 63142842 ID=Msa0083980-mRNA-1;Parent=Msa0083980;Name=Msa0083980-mRNA-1;_AED=0.02;_eAED=0.02;_QI=257|1|1|1|0.75|0.8|5|504|305
chr1_2 exon 63142842 63143603 63142842 ID=Msa0083980-mRNA-1:exon:21103;Parent=Msa0083980-mRNA-1
chr1_2 exon 63144220 63144341 63144220 ID=Msa0083980-mRNA-1:exon:21104;Parent=Msa0083980-mRNA-1
chr1_2 exon 63144457 63144497 63144457 ID=Msa0083980-mRNA-1:exon:21105;Parent=Msa0083980-mRNA-1
chr1_2 exon 63144598 63144696 63144598 ID=Msa0083980-mRNA-1:exon:21106;Parent=Msa0083980-mRNA-1
chr1_2 exon 63145119 63145773 63145119 ID=Msa0083980-mRNA-1:exon:21107;Parent=Msa0083980-mRNA-1
chr1_2 five_prime_UTR 63142842 63143098 63142842 ID=Msa0083980-mRNA-1:five_prime_utr;Parent=Msa0083980-mRNA-1
chr1_2 CDS 63143099 63143603 63143099 ID=Msa0083980-mRNA-1:cds;Parent=Msa0083980-mRNA-1
chr1_2 CDS 63144220 63144341 63144220 ID=Msa0083980-mRNA-1:cds;Parent=Msa0083980-mRNA-1
chr1_2 CDS 63144457 63144497 63144457 ID=Msa0083980-mRNA-1:cds;Parent=Msa0083980-mRNA-1
chr1_2 CDS 63144598 63144696 63144598 ID=Msa0083980-mRNA-1:cds;Parent=Msa0083980-mRNA-1
chr1_2 CDS 63145119 63145269 63145119 ID=Msa0083980-mRNA-1:cds;Parent=Msa0083980-mRNA-1
chr1_2 three_prime_UTR 63145270 63145773 63145270 ID=Msa0083980-mRNA-1:three_prime_utr;Parent=Msa0083980-mRNA-1
Gene Sequence

>Msa0083980

ATGCTTGGTGTACTCTGCGCCACTCGCTCTCGTCCTTGGATCTTCTCTTCCCACCACCACCACCACGCCTTTCGTCTCTCTCACGCCACTGTCGCTCCTCTCACTTTCCCCGCCCGCCGCCACCATTCCACCGCCTGCAACCACCTCCAAATCTCAACCGGCGGTGGAGCTGCTTCGATATGGCACGCGATTACTCCTTGCGGCGGCGATGGATTCCGAAGCAGCGGTGTGATGCTTCATCATGACCACGAGCTCAAAGGTGAAGGATCGTGGAACGTTGCTTGGGATGCTCGTCCTGCTAGGTGGCTTCATCGTTCTGACTCAGCTTGGCTTCTTTTCGGTGTCTGCGCTTGCCTTGCCCCGCCGGTTATTGTTGACGTTGATACCGAGGCGCCGCCGCCGACTCCAGCTGTTTTCCCGGACGAGAGTTCCGAGGGACGTGAAGTGAAAGATGAGCTTTCCGATGAACGTGACGATGAACTCAACGCTGATTACAGAGTCACGGGGGTGCTTGCTGATGGTCGGTGTCTGTTCAGAGCAATAGCTCATGGAGCTTGCTTGAAAAATGGAGAGGAAGCGCCTAATGAAAGTCGACAGAGGGAACTTGCCGACGAATTAAGGGTTAAAGTTGCTGAAGAACTTTTGAACAGGCGCAAGGAAACAGAATGGTTTATAGAAGGGGATTTTGACACATATGTGACCAGAATTCAACAGACTTATGTTTGGGGAGGAGAGCCTGAGCTATTAATGGCTTCTCATGTTTTGAAGACTCCAATATATGTCTTCATGAGAGATGCAAGCTCCATGGATTTGGTAAATATAGCAAAATACGGTGAAGAATATATGAATGATGAGGAGATTTCCATCAATGTGCTATTTCATCGACATGGTCACTACGAACTATTGGAGACCTTATGA
Protein sequence

>Msa0083980

MLGVLCATRSRPWIFSSHHHHHAFRLSHATVAPLTFPARRHHSTACNHLQISTGGGAASIWHAITPCGGDGFRSSGVMLHHDHELKGEGSWNVAWDARPARWLHRSDSAWLLFGVCACLAPPVIVDVDTEAPPPTPAVFPDESSEGREVKDELSDERDDELNADYRVTGVLADGRCLFRAIAHGACLKNGEEAPNESRQRELADELRVKVAEELLNRRKETEWFIEGDFDTYVTRIQQTYVWGGEPELLMASHVLKTPIYVFMRDASSMDLVNIAKYGEEYMNDEEISINVLFHRHGHYELLETL*