AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0035980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035980 MtrunA17_Chr1g0198131 96.721 305 10 0 1 305 1 305 0.0 615
Msa0035980 MtrunA17_Chr1g0174241 53.254 169 71 2 142 304 161 327 4.98e-58 189
Msa0035980 MtrunA17_Chr3g0122741 49.007 151 73 2 153 303 2 148 1.13e-48 159
Msa0035980 MtrunA17_Chr1g0174221 47.945 146 73 2 159 303 91 234 1.37e-42 147
Msa0035980 MtrunA17_Chr4g0005721 35.294 153 83 5 165 302 77 228 6.08e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0035980 AT3G57810.2 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0035980 AT3G57810.4 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0035980 AT3G57810.3 58.571 140 56 1 163 302 166 303 1.50e-55 182
Msa0035980 AT2G38025.2 32.075 159 86 4 165 302 53 210 4.69e-15 73.2
Msa0035980 AT2G38025.1 32.075 159 86 4 165 302 76 233 8.56e-15 72.8
Msa0035980 AT3G57810.1 39.362 94 52 2 123 211 121 214 2.68e-14 71.2

Find 129 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACTTGCCGACGAATTA+AGG 0.213414 1_1:+64302886 Msa0035980:CDS
ATCATGCGGTATAAGTCTTC+TGG 0.238749 1_1:+64303899 Msa0035980:three_prime_UTR
CTTAATAATAAAACCCGTTA+TGG 0.281750 1_1:+64301581 Msa0035980:five_prime_UTR
CTCCACCGCCGGTTGAGATT+TGG 0.298384 1_1:-64301818 None:intergenic
AACATAAGTCTGTTGAATTC+TGG 0.316175 1_1:-64303202 None:intergenic
TGGAATGGTGGCGGCGGGCG+GGG 0.325826 1_1:-64301779 None:intergenic
TGAGATTTGGAGGTGGTTGC+AGG 0.327466 1_1:-64301805 None:intergenic
GGTGGAATGGTGGCGGCGGG+CGG 0.330689 1_1:-64301781 None:intergenic
AATTCAACAGACTTATGTTT+GGG 0.341402 1_1:+64303206 Msa0035980:CDS
TCTCTCATGAAGACATATAT+TGG 0.349253 1_1:-64303691 None:intergenic
GTGGAATGGTGGCGGCGGGC+GGG 0.352247 1_1:-64301780 None:intergenic
GCGCCACTCGCTCTCGTCCT+TGG 0.358149 1_1:+64301688 Msa0035980:CDS
AGATGCAAGCTCCATGGATT+TGG 0.364917 1_1:+64303711 Msa0035980:CDS
AGGAGAGCCTGAGCTATTAA+TGG 0.369529 1_1:+64303230 Msa0035980:CDS
AGAGAAGCGTGGGTGTTATA+AGG 0.369781 1_1:-64301485 None:intergenic
CTGCGCTTGCCTTGCGCCGC+CGG 0.373717 1_1:+64302016 Msa0035980:CDS
ACTGCAAAACAAAAGTTCAT+TGG 0.380213 1_1:-64304020 None:intergenic
GAAAGGCGTGGTGGTGGTGG+TGG 0.384833 1_1:-64301722 None:intergenic
ACATGGTCACTACGAACTAT+TGG 0.387672 1_1:+64303804 Msa0035980:CDS
TTCCATTCCAAGAAAAGAAA+AGG 0.389727 1_1:+64301451 Msa0035980:five_prime_UTR
TGGAGTCGGCGGCGGCGCCT+CGG 0.390531 1_1:-64302057 None:intergenic
GAATTCAACAGACTTATGTT+TGG 0.396680 1_1:+64303205 Msa0035980:CDS
GCTACTGGCATTTGAGCTGC+AGG 0.412012 1_1:+64303982 Msa0035980:three_prime_UTR
CAAAAGTGGACATTGTTGTT+TGG 0.413224 1_1:+64301428 Msa0035980:five_prime_UTR
TGTCAGCATAAGAACTTCAT+TGG 0.413335 1_1:-64304199 None:intergenic
TCGATGAAATAGCACATTGA+TGG 0.414542 1_1:-64303782 None:intergenic
GGCGGTGGAATGGTGGCGGC+GGG 0.418826 1_1:-64301784 None:intergenic
GGAAGGTTTAGGTGAGTTGG+TGG 0.420220 1_1:+64304063 Msa0035980:three_prime_UTR
ATCTTTCACTTCACGTCCCT+CGG 0.423234 1_1:-64302102 None:intergenic
GAAGCATCACACCGCTGCTT+CGG 0.424353 1_1:-64301887 None:intergenic
TCCCTCTGTCGACTTTCATT+AGG 0.424371 1_1:-64302867 None:intergenic
GCACTGTGTATGTGAGTGTA+TGG 0.425283 1_1:-64301626 None:intergenic
CTCTCGTCCGGGAAAACAGC+TGG 0.428780 1_1:-64302077 None:intergenic
TTGGAGGTGGTTGCAGGCGG+TGG 0.429035 1_1:-64301799 None:intergenic
AATGGAAGGTTTAGGTGAGT+TGG 0.429406 1_1:+64304060 Msa0035980:three_prime_UTR
TGCTCGATTCTTGATACTCA+TGG 0.435244 1_1:+64304118 Msa0035980:three_prime_UTR
ATTACTCCTTGCGGCGGCGA+TGG 0.436846 1_1:+64301861 Msa0035980:CDS
TTAATAATAAAACCCGTTAT+GGG 0.437129 1_1:+64301582 Msa0035980:five_prime_UTR
GGAACTTGCCGACGAATTAA+GGG 0.438594 1_1:+64302887 Msa0035980:CDS
CGTCCCTCGGAACTCTCGTC+CGG 0.439061 1_1:-64302089 None:intergenic
TTATCCTACGCACCCATAAC+GGG 0.440177 1_1:-64301594 None:intergenic
GAGTTCATCGTCACGTTCAT+CGG 0.445917 1_1:-64302132 None:intergenic
AAGGATCGTGGAACGTTGCT+TGG 0.447140 1_1:+64301934 Msa0035980:CDS
TTCATCGTTCTGACTCAGCT+TGG 0.448002 1_1:+64301979 Msa0035980:CDS
TGCTATATTTACCAAATCCA+TGG 0.450384 1_1:-64303722 None:intergenic
TGTGTCTATAGGTTTATAGA+AGG 0.457572 1_1:+64303159 Msa0035980:intron
TCATGCGGTATAAGTCTTCT+GGG 0.458739 1_1:+64303900 Msa0035980:three_prime_UTR
GCGGTGGAGCTGCTTCGATA+TGG 0.467969 1_1:+64301832 Msa0035980:CDS
GACGAAAGGCGTGGTGGTGG+TGG 0.470943 1_1:-64301725 None:intergenic
AGGCGGTGGAATGGTGGCGG+CGG 0.476240 1_1:-64301785 None:intergenic
CACCGCCGGTTGAGATTTGG+AGG 0.479044 1_1:-64301815 None:intergenic
GTATCAACGTCAACAATAAC+CGG 0.479760 1_1:-64302035 None:intergenic
CTTGGGATGCTCGTCCTGCT+AGG 0.480235 1_1:+64301952 Msa0035980:CDS
TACTTTAACCCTTAATTCGT+CGG 0.484358 1_1:-64302895 None:intergenic
ATATGCAATCAATGTACAAA+AGG 0.492528 1_1:-64304286 None:intergenic
AAAGGCGTGGTGGTGGTGGT+GGG 0.493789 1_1:-64301721 None:intergenic
CTCCAAATCTCAACCGGCGG+TGG 0.496363 1_1:+64301816 Msa0035980:CDS
GGTGGTTGCAGGCGGTGGAA+TGG 0.499927 1_1:-64301794 None:intergenic
GCCACTCACTCATCCATGCT+TGG 0.503058 1_1:+64301657 Msa0035980:exon
ATCCTTCACCTTTGAGCTCG+TGG 0.505031 1_1:-64301917 None:intergenic
TTGGTAAATATAGCAAAATA+CGG 0.508333 1_1:+64303730 Msa0035980:CDS
AGAGAAGAGAGCAATAAGAA+TGG 0.511242 1_1:-64301534 None:intergenic
CGCCGGTTGAGATTTGGAGG+TGG 0.513387 1_1:-64301812 None:intergenic
CACTGTGTATGTGAGTGTAT+GGG 0.514314 1_1:-64301625 None:intergenic
TGAGAAGCCATTAATAGCTC+AGG 0.515628 1_1:-64303237 None:intergenic
AAAACAGCTGGAGTCGGCGG+CGG 0.517656 1_1:-64302065 None:intergenic
AACCACCTCCAAATCTCAAC+CGG 0.518771 1_1:+64301810 Msa0035980:CDS
GTTATCCTACGCACCCATAA+CGG 0.524419 1_1:-64301595 None:intergenic
AGGATCGTGGAACGTTGCTT+GGG 0.525778 1_1:+64301935 Msa0035980:CDS
CTACTGGCATTTGAGCTGCA+GGG 0.526098 1_1:+64303983 Msa0035980:three_prime_UTR
TGCAGGCGGTGGAATGGTGG+CGG 0.528482 1_1:-64301788 None:intergenic
CGGCGGGCGGGGAAAGTGAG+AGG 0.534229 1_1:-64301768 None:intergenic
GCTCGATTCTTGATACTCAT+GGG 0.538567 1_1:+64304119 Msa0035980:three_prime_UTR
GTCCCTCGGAACTCTCGTCC+GGG 0.539858 1_1:-64302088 None:intergenic
CTGTTCAGAGCAATAGCTCA+TGG 0.539965 1_1:+64302816 Msa0035980:CDS
CAAAAGGAGCCAACGAATGT+TGG 0.540663 1_1:-64304270 None:intergenic
TTAAGTGAACCAACATTCGT+TGG 0.542574 1_1:+64304261 Msa0035980:three_prime_UTR
GTGTCTATAGGTTTATAGAA+GGG 0.543445 1_1:+64303160 Msa0035980:intron
TGTCTATAGGTTTATAGAAG+GGG 0.544260 1_1:+64303161 Msa0035980:intron
GAACGATGAAGCCACCTAGC+AGG 0.545883 1_1:-64301966 None:intergenic
GGTTGCAGGCGGTGGAATGG+TGG 0.547561 1_1:-64301791 None:intergenic
TAGGGGTGCTTGCTGATGGT+CGG 0.550798 1_1:+64302790 Msa0035980:intron
TCCATTCCAAGAAAAGAAAA+GGG 0.553262 1_1:+64301452 Msa0035980:five_prime_UTR
CAACAATAACCGGCGGCGCA+AGG 0.554275 1_1:-64302025 None:intergenic
CGGAATCCATCGCCGCCGCA+AGG 0.559044 1_1:-64301867 None:intergenic
GGCGCAGAGTACACCAAGCA+TGG 0.563817 1_1:-64301670 None:intergenic
AGCTCAAAGGTGAAGGATCG+TGG 0.569548 1_1:+64301922 Msa0035980:CDS
AACGCTGATTACAGAGTCAC+GGG 0.570421 1_1:+64302155 Msa0035980:CDS
CATGAGAGATGCAAGCTCCA+TGG 0.570844 1_1:+64303705 Msa0035980:CDS
GAAGAGAAAGAGAGAAGCGT+GGG 0.573463 1_1:-64301495 None:intergenic
TCCGGGAAAACAGCTGGAGT+CGG 0.574081 1_1:-64302071 None:intergenic
CAACAGACTTATGTTTGGGG+AGG 0.576052 1_1:+64303210 Msa0035980:CDS
GACCACGAGCTCAAAGGTGA+AGG 0.577751 1_1:+64301915 Msa0035980:CDS
AAAACCCGTTATGGGTGCGT+AGG 0.577803 1_1:+64301590 Msa0035980:five_prime_UTR
GATCCAAGGACGAGAGCGAG+TGG 0.579496 1_1:-64301691 None:intergenic
GGTATAGGGGTGCTTGCTGA+TGG 0.584649 1_1:+64302786 Msa0035980:intron
TATCGAAGCAGCTCCACCGC+CGG 0.586713 1_1:-64301829 None:intergenic
CATCATGACCACGAGCTCAA+AGG 0.587256 1_1:+64301909 Msa0035980:CDS
TTCCCGGACGAGAGTTCCGA+GGG 0.588950 1_1:+64302086 Msa0035980:CDS
AGAGACGAAAGGCGTGGTGG+TGG 0.589008 1_1:-64301728 None:intergenic
AGTCTTAAAGAACACTTGCA+TGG 0.592866 1_1:+64304158 Msa0035980:three_prime_UTR
ATTTAGTTTCTTTAATCATG+CGG 0.595658 1_1:+64303885 Msa0035980:three_prime_UTR
GAAAGTGAGAGGAGCGACAG+TGG 0.606183 1_1:-64301757 None:intergenic
CAACGCTGATTACAGAGTCA+CGG 0.607136 1_1:+64302154 Msa0035980:CDS
CACGCGATTACTCCTTGCGG+CGG 0.611520 1_1:+64301855 Msa0035980:CDS
TTTCCCGGACGAGAGTTCCG+AGG 0.612131 1_1:+64302085 Msa0035980:CDS
GCCTAATGAAAGTCGACAGA+GGG 0.612158 1_1:+64302866 Msa0035980:CDS
CGAAGAGAAAGAGAGAAGCG+TGG 0.621048 1_1:-64301496 None:intergenic
GGCGATGGATTCCGAAGCAG+CGG 0.621773 1_1:+64301876 Msa0035980:CDS
TCCAAGAAAAGAAAAGGGTG+AGG 0.622110 1_1:+64301457 Msa0035980:five_prime_UTR
TATTGTTGACGTTGATACCG+AGG 0.628764 1_1:+64302040 Msa0035980:CDS
GGGAAAACAGCTGGAGTCGG+CGG 0.628808 1_1:-64302068 None:intergenic
TGAAGAATATATGAATGATG+AGG 0.630448 1_1:+64303753 Msa0035980:CDS
ACCAAGCATGGATGAGTGAG+TGG 0.631102 1_1:-64301658 None:intergenic
GATTTGGAGGTGGTTGCAGG+CGG 0.637060 1_1:-64301802 None:intergenic
ACTGTGTATGTGAGTGTATG+GGG 0.640581 1_1:-64301624 None:intergenic
TGGTGGGAAGAGAAGATCCA+AGG 0.650314 1_1:-64301705 None:intergenic
CGCCTAATGAAAGTCGACAG+AGG 0.650904 1_1:+64302865 Msa0035980:CDS
CACCTCCAAATCTCAACCGG+CGG 0.653859 1_1:+64301813 Msa0035980:CDS
GGGATGCTCGTCCTGCTAGG+TGG 0.655002 1_1:+64301955 Msa0035980:CDS
ACAGGCGCAAGGAAACAGAA+TGG 0.661069 1_1:+64303048 Msa0035980:CDS
ATTCAACAGACTTATGTTTG+GGG 0.663997 1_1:+64303207 Msa0035980:CDS
AATGTGCTATTTCATCGACA+TGG 0.668256 1_1:+64303787 Msa0035980:CDS
GAAAAGAAAAGGGTGAGGTG+AGG 0.669407 1_1:+64301462 Msa0035980:five_prime_UTR
GAGAGAGACGAAAGGCGTGG+TGG 0.678901 1_1:-64301731 None:intergenic
CGTGAGAGAGACGAAAGGCG+TGG 0.686067 1_1:-64301734 None:intergenic
AGTGGCGTGAGAGAGACGAA+AGG 0.697149 1_1:-64301739 None:intergenic
TGGCACGCGATTACTCCTTG+CGG 0.725561 1_1:+64301852 Msa0035980:CDS
TCAACGTCAACAATAACCGG+CGG 0.746562 1_1:-64302032 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAACATAACAAAAATT+GGG - chr1_1:64303006-64303025 None:intergenic 10.0%
!! AAATAAAACATAACAAAAAT+TGG - chr1_1:64303007-64303026 None:intergenic 10.0%
!!! ATTTTTGTTATGTTTTATTT+AGG + chr1_1:64303007-64303026 Msa0035980:intron 10.0%
!!! TTATTTTACTTTTATTTACA+TGG + chr1_1:64303115-64303134 Msa0035980:intron 10.0%
!!! TTTTTGTATTTTCTTTTTAA+TGG + chr1_1:64304042-64304061 Msa0035980:three_prime_UTR 10.0%
!!! TATTTTTTTCATATAAGTAC+AGG + chr1_1:64302359-64302378 Msa0035980:intron 15.0%
!!! ATTTTTTTCATATAAGTACA+GGG + chr1_1:64302360-64302379 Msa0035980:intron 15.0%
!!! TTTTTTTTTTTTTTGAAAGG+AGG + chr1_1:64304090-64304109 Msa0035980:three_prime_UTR 15.0%
!! TTAATAATAAAACCCGTTAT+GGG + chr1_1:64301582-64301601 Msa0035980:five_prime_UTR 20.0%
!!! TTATTGTTCAGTTATTTTGA+TGG + chr1_1:64302694-64302713 Msa0035980:intron 20.0%
!! AAATTCATTGAAGGAAAAAA+AGG - chr1_1:64302978-64302997 None:intergenic 20.0%
!! TTGGTAAATATAGCAAAATA+CGG + chr1_1:64303730-64303749 Msa0035980:CDS 20.0%
!!! ATTTAGTTTCTTTAATCATG+CGG + chr1_1:64303885-64303904 Msa0035980:three_prime_UTR 20.0%
!!! TGTATTTTCTTTTTAATGGA+AGG + chr1_1:64304046-64304065 Msa0035980:three_prime_UTR 20.0%
!!! TTCTCTTCGTTCTTTTTTAT+TGG + chr1_1:64301510-64301529 Msa0035980:five_prime_UTR 25.0%
!!! ACGGGTTTTATTATTAAGAT+TGG - chr1_1:64301579-64301598 None:intergenic 25.0%
! CTTAATAATAAAACCCGTTA+TGG + chr1_1:64301581-64301600 Msa0035980:five_prime_UTR 25.0%
! AACAAGAACTATTGTATCAT+TGG - chr1_1:64302608-64302627 None:intergenic 25.0%
! TATAAGTTTGTATCACTGAA+TGG + chr1_1:64302765-64302784 Msa0035980:intron 25.0%
! GGAAACTACATACAATTATT+TGG + chr1_1:64303089-64303108 Msa0035980:intron 25.0%
! AATTCAACAGACTTATGTTT+GGG + chr1_1:64303206-64303225 Msa0035980:CDS 25.0%
! TAATGAAAGATGATAATCGA+TGG + chr1_1:64303478-64303497 Msa0035980:intron 25.0%
! AATCGATGGATATTAATCTA+CGG + chr1_1:64303492-64303511 Msa0035980:intron 25.0%
! CTTATAGAGAAACAAATTTC+CGG + chr1_1:64303549-64303568 Msa0035980:intron 25.0%
!! TTCATTTTGTGGTAAATCAT+TGG + chr1_1:64303572-64303591 Msa0035980:intron 25.0%
!! TCATTTTGTGGTAAATCATT+GGG + chr1_1:64303573-64303592 Msa0035980:intron 25.0%
! TAAGTTATGAAGACAATGTT+AGG - chr1_1:64303663-64303682 None:intergenic 25.0%
! TGAAGAATATATGAATGATG+AGG + chr1_1:64303753-64303772 Msa0035980:CDS 25.0%
!!! GTTATTTTTGAGACAACTTT+TGG - chr1_1:64303841-64303860 None:intergenic 25.0%
!!! AAGAGCTTTTTTTGTCATTT+GGG + chr1_1:64303928-64303947 Msa0035980:three_prime_UTR 25.0%
!!! TTTAGTAGTTTTTTTGCTAC+TGG + chr1_1:64303967-64303986 Msa0035980:three_prime_UTR 25.0%
!! TTCTTTTTAATGGAAGGTTT+AGG + chr1_1:64304052-64304071 Msa0035980:three_prime_UTR 25.0%
! ATATGCAATCAATGTACAAA+AGG - chr1_1:64304289-64304308 None:intergenic 25.0%
TTCCATTCCAAGAAAAGAAA+AGG + chr1_1:64301451-64301470 Msa0035980:five_prime_UTR 30.0%
TCCATTCCAAGAAAAGAAAA+GGG + chr1_1:64301452-64301471 Msa0035980:five_prime_UTR 30.0%
TTTCATATAAGTACAGGGAT+TGG + chr1_1:64302365-64302384 Msa0035980:intron 30.0%
TTACAACTACGAAACAACAA+CGG + chr1_1:64302523-64302542 Msa0035980:intron 30.0%
ACTTGTTCAATATGAAGTGT+CGG + chr1_1:64302645-64302664 Msa0035980:intron 30.0%
AACATGAGTTAGAGATTATG+TGG + chr1_1:64302735-64302754 Msa0035980:intron 30.0%
ACATGAGTTAGAGATTATGT+GGG + chr1_1:64302736-64302755 Msa0035980:intron 30.0%
TTTGTATCACTGAATGGTAT+AGG + chr1_1:64302771-64302790 Msa0035980:intron 30.0%
TTGTATCACTGAATGGTATA+GGG + chr1_1:64302772-64302791 Msa0035980:intron 30.0%
TACTTTAACCCTTAATTCGT+CGG - chr1_1:64302898-64302917 None:intergenic 30.0%
!! TCTTGCTAGAACTTTTGAAA+CGG + chr1_1:64302924-64302943 Msa0035980:intron 30.0%
TGGGTAACAAAATTCATTGA+AGG - chr1_1:64302987-64303006 None:intergenic 30.0%
!! GTTATATTTGCTGTGTCTAT+AGG + chr1_1:64303148-64303167 Msa0035980:intron 30.0%
TGTGTCTATAGGTTTATAGA+AGG + chr1_1:64303159-64303178 Msa0035980:intron 30.0%
GTGTCTATAGGTTTATAGAA+GGG + chr1_1:64303160-64303179 Msa0035980:intron 30.0%
TGTCTATAGGTTTATAGAAG+GGG + chr1_1:64303161-64303180 Msa0035980:intron 30.0%
AACATAAGTCTGTTGAATTC+TGG - chr1_1:64303205-64303224 None:intergenic 30.0%
GAATTCAACAGACTTATGTT+TGG + chr1_1:64303205-64303224 Msa0035980:CDS 30.0%
ATTCAACAGACTTATGTTTG+GGG + chr1_1:64303207-64303226 Msa0035980:CDS 30.0%
AACATCATATCCTACAACAA+CGG + chr1_1:64303411-64303430 Msa0035980:intron 30.0%
ATCGATGGATATTAATCTAC+GGG + chr1_1:64303493-64303512 Msa0035980:intron 30.0%
TGATTTACCACAAAATGAAC+CGG - chr1_1:64303571-64303590 None:intergenic 30.0%
CATTGTCTTCATAACTTACA+AGG + chr1_1:64303665-64303684 Msa0035980:intron 30.0%
TCTCTCATGAAGACATATAT+TGG - chr1_1:64303694-64303713 None:intergenic 30.0%
TGCTATATTTACCAAATCCA+TGG - chr1_1:64303725-64303744 None:intergenic 30.0%
!!! GAAGAGCTTTTTTTGTCATT+TGG + chr1_1:64303927-64303946 Msa0035980:three_prime_UTR 30.0%
!!! AGAGCTTTTTTTGTCATTTG+GGG + chr1_1:64303929-64303948 Msa0035980:three_prime_UTR 30.0%
ACTGCAAAACAAAAGTTCAT+TGG - chr1_1:64304023-64304042 None:intergenic 30.0%
! CAAAAGTGGACATTGTTGTT+TGG + chr1_1:64301428-64301447 Msa0035980:five_prime_UTR 35.0%
! ACCCTTTTCTTTTCTTGGAA+TGG - chr1_1:64301456-64301475 None:intergenic 35.0%
AGAGAAGAGAGCAATAAGAA+TGG - chr1_1:64301537-64301556 None:intergenic 35.0%
GTATCAACGTCAACAATAAC+CGG - chr1_1:64302038-64302057 None:intergenic 35.0%
! ATATAAGTACAGGGATTGGT+TGG + chr1_1:64302369-64302388 Msa0035980:intron 35.0%
ATTGAACAAGTGTCTTGAGT+TGG - chr1_1:64302636-64302655 None:intergenic 35.0%
TGTATCACTGAATGGTATAG+GGG + chr1_1:64302773-64302792 Msa0035980:intron 35.0%
AAGGATGTTGACAAAGAAGA+AGG - chr1_1:64302959-64302978 None:intergenic 35.0%
! TTGCTGAAGAACTTTTGAAC+AGG + chr1_1:64303030-64303049 Msa0035980:CDS 35.0%
ACAAGTTAAACCGTTGTTGT+AGG - chr1_1:64303424-64303443 None:intergenic 35.0%
!! AACAACGGTTTAACTTGTTG+TGG + chr1_1:64303426-64303445 Msa0035980:intron 35.0%
! CAAATTTCCGGTTCATTTTG+TGG + chr1_1:64303561-64303580 Msa0035980:intron 35.0%
GAAGACAATGTTAGGTACAA+CGG - chr1_1:64303655-64303674 None:intergenic 35.0%
TCGATGAAATAGCACATTGA+TGG - chr1_1:64303785-64303804 None:intergenic 35.0%
!! AATGTGCTATTTCATCGACA+TGG + chr1_1:64303787-64303806 Msa0035980:CDS 35.0%
! TGAGACAACTTTTGGTCATA+AGG - chr1_1:64303833-64303852 None:intergenic 35.0%
AGTCTTAAAGAACACTTGCA+TGG + chr1_1:64304158-64304177 Msa0035980:three_prime_UTR 35.0%
TGTCAGCATAAGAACTTCAT+TGG - chr1_1:64304202-64304221 None:intergenic 35.0%
TTAAGTGAACCAACATTCGT+TGG + chr1_1:64304261-64304280 Msa0035980:three_prime_UTR 35.0%
TCCAAGAAAAGAAAAGGGTG+AGG + chr1_1:64301457-64301476 Msa0035980:five_prime_UTR 40.0%
! ACCTCACCCTTTTCTTTTCT+TGG - chr1_1:64301461-64301480 None:intergenic 40.0%
ACTGTGTATGTGAGTGTATG+GGG - chr1_1:64301627-64301646 None:intergenic 40.0%
CACTGTGTATGTGAGTGTAT+GGG - chr1_1:64301628-64301647 None:intergenic 40.0%
TATTGTTGACGTTGATACCG+AGG + chr1_1:64302040-64302059 Msa0035980:CDS 40.0%
GTTTGTGACCAACTGTTTGA+TGG + chr1_1:64302483-64302502 Msa0035980:intron 40.0%
GTGACAAACCATCAAACAGT+TGG - chr1_1:64302494-64302513 None:intergenic 40.0%
! CATGGAGCTTGCTTGAAAAA+TGG + chr1_1:64302834-64302853 Msa0035980:CDS 40.0%
! AGCTTGCTTGAAAAATGGAG+AGG + chr1_1:64302839-64302858 Msa0035980:CDS 40.0%
!!! CTAGAACTTTTGAAACGGAG+AGG + chr1_1:64302929-64302948 Msa0035980:intron 40.0%
! TGGTGAGCTTTGTCATTTGA+AGG + chr1_1:64303068-64303087 Msa0035980:intron 40.0%
TGAGAAGCCATTAATAGCTC+AGG - chr1_1:64303240-64303259 None:intergenic 40.0%
TGTGGTAAATCATTGGGACT+TGG + chr1_1:64303579-64303598 Msa0035980:intron 40.0%
GTGGTAAATCATTGGGACTT+GGG + chr1_1:64303580-64303599 Msa0035980:intron 40.0%
TGGTAAATCATTGGGACTTG+GGG + chr1_1:64303581-64303600 Msa0035980:intron 40.0%
CAATGTTAGGTACAACGGAA+TGG - chr1_1:64303650-64303669 None:intergenic 40.0%
ACATGGTCACTACGAACTAT+TGG + chr1_1:64303804-64303823 Msa0035980:CDS 40.0%
ATCATGCGGTATAAGTCTTC+TGG + chr1_1:64303899-64303918 Msa0035980:three_prime_UTR 40.0%
TCATGCGGTATAAGTCTTCT+GGG + chr1_1:64303900-64303919 Msa0035980:three_prime_UTR 40.0%
AATGGAAGGTTTAGGTGAGT+TGG + chr1_1:64304060-64304079 Msa0035980:three_prime_UTR 40.0%
TGCTCGATTCTTGATACTCA+TGG + chr1_1:64304118-64304137 Msa0035980:three_prime_UTR 40.0%
GCTCGATTCTTGATACTCAT+GGG + chr1_1:64304119-64304138 Msa0035980:three_prime_UTR 40.0%
GAAAAGAAAAGGGTGAGGTG+AGG + chr1_1:64301462-64301481 Msa0035980:five_prime_UTR 45.0%
! AGAGAAGCGTGGGTGTTATA+AGG - chr1_1:64301488-64301507 None:intergenic 45.0%
! GAAGAGAAAGAGAGAAGCGT+GGG - chr1_1:64301498-64301517 None:intergenic 45.0%
TTATCCTACGCACCCATAAC+GGG - chr1_1:64301597-64301616 None:intergenic 45.0%
GTTATCCTACGCACCCATAA+CGG - chr1_1:64301598-64301617 None:intergenic 45.0%
GCACTGTGTATGTGAGTGTA+TGG - chr1_1:64301629-64301648 None:intergenic 45.0%
AACCACCTCCAAATCTCAAC+CGG + chr1_1:64301810-64301829 Msa0035980:CDS 45.0%
TTCATCGTTCTGACTCAGCT+TGG + chr1_1:64301979-64301998 Msa0035980:CDS 45.0%
! GACTCAGCTTGGCTTCTTTT+CGG + chr1_1:64301990-64302009 Msa0035980:CDS 45.0%
TCAACGTCAACAATAACCGG+CGG - chr1_1:64302035-64302054 None:intergenic 45.0%
ATCTTTCACTTCACGTCCCT+CGG - chr1_1:64302105-64302124 None:intergenic 45.0%
GAGTTCATCGTCACGTTCAT+CGG - chr1_1:64302135-64302154 None:intergenic 45.0%
! CAACGCTGATTACAGAGTCA+CGG + chr1_1:64302154-64302173 Msa0035980:CDS 45.0%
! AACGCTGATTACAGAGTCAC+GGG + chr1_1:64302155-64302174 Msa0035980:CDS 45.0%
!! TAAGTACAGGGATTGGTTGG+TGG + chr1_1:64302372-64302391 Msa0035980:intron 45.0%
ACGAAACAACAACGGACATC+AGG + chr1_1:64302531-64302550 Msa0035980:intron 45.0%
CTGTTCAGAGCAATAGCTCA+TGG + chr1_1:64302816-64302835 Msa0035980:CDS 45.0%
GCCTAATGAAAGTCGACAGA+GGG + chr1_1:64302866-64302885 Msa0035980:CDS 45.0%
!! TCCCTCTGTCGACTTTCATT+AGG - chr1_1:64302870-64302889 None:intergenic 45.0%
GGAACTTGCCGACGAATTAA+GGG + chr1_1:64302887-64302906 Msa0035980:CDS 45.0%
! AGAACTTTTGAACAGGCGCA+AGG + chr1_1:64303037-64303056 Msa0035980:CDS 45.0%
CAACAGACTTATGTTTGGGG+AGG + chr1_1:64303210-64303229 Msa0035980:CDS 45.0%
AGGAGAGCCTGAGCTATTAA+TGG + chr1_1:64303230-64303249 Msa0035980:CDS 45.0%
GGTAAATCATTGGGACTTGG+GGG + chr1_1:64303582-64303601 Msa0035980:intron 45.0%
AGATGCAAGCTCCATGGATT+TGG + chr1_1:64303711-64303730 Msa0035980:CDS 45.0%
CAAAAGGAGCCAACGAATGT+TGG - chr1_1:64304273-64304292 None:intergenic 45.0%
!!! TTTTTTTTTTTTTTTTTGAA+AGG + chr1_1:64304087-64304106 Msa0035980:three_prime_UTR 5.0%
CGAAGAGAAAGAGAGAAGCG+TGG - chr1_1:64301499-64301518 None:intergenic 50.0%
AAAACCCGTTATGGGTGCGT+AGG + chr1_1:64301590-64301609 Msa0035980:five_prime_UTR 50.0%
ACCAAGCATGGATGAGTGAG+TGG - chr1_1:64301661-64301680 None:intergenic 50.0%
TGGTGGGAAGAGAAGATCCA+AGG - chr1_1:64301708-64301727 None:intergenic 50.0%
TGAGATTTGGAGGTGGTTGC+AGG - chr1_1:64301808-64301827 None:intergenic 50.0%
CATCATGACCACGAGCTCAA+AGG + chr1_1:64301909-64301928 Msa0035980:CDS 50.0%
ATCCTTCACCTTTGAGCTCG+TGG - chr1_1:64301920-64301939 None:intergenic 50.0%
AGCTCAAAGGTGAAGGATCG+TGG + chr1_1:64301922-64301941 Msa0035980:CDS 50.0%
AAGGATCGTGGAACGTTGCT+TGG + chr1_1:64301934-64301953 Msa0035980:CDS 50.0%
AGGATCGTGGAACGTTGCTT+GGG + chr1_1:64301935-64301954 Msa0035980:CDS 50.0%
CGCCTAATGAAAGTCGACAG+AGG + chr1_1:64302865-64302884 Msa0035980:CDS 50.0%
GGGAACTTGCCGACGAATTA+AGG + chr1_1:64302886-64302905 Msa0035980:CDS 50.0%
ACAGGCGCAAGGAAACAGAA+TGG + chr1_1:64303048-64303067 Msa0035980:CDS 50.0%
CATGAGAGATGCAAGCTCCA+TGG + chr1_1:64303705-64303724 Msa0035980:CDS 50.0%
CTACTGGCATTTGAGCTGCA+GGG + chr1_1:64303983-64304002 Msa0035980:three_prime_UTR 50.0%
! GGAAGGTTTAGGTGAGTTGG+TGG + chr1_1:64304063-64304082 Msa0035980:three_prime_UTR 50.0%
GCCACTCACTCATCCATGCT+TGG + chr1_1:64301657-64301676 Msa0035980:exon 55.0%
AGTGGCGTGAGAGAGACGAA+AGG - chr1_1:64301742-64301761 None:intergenic 55.0%
GAAAGTGAGAGGAGCGACAG+TGG - chr1_1:64301760-64301779 None:intergenic 55.0%
GATTTGGAGGTGGTTGCAGG+CGG - chr1_1:64301805-64301824 None:intergenic 55.0%
CACCTCCAAATCTCAACCGG+CGG + chr1_1:64301813-64301832 Msa0035980:CDS 55.0%
TGGCACGCGATTACTCCTTG+CGG + chr1_1:64301852-64301871 Msa0035980:CDS 55.0%
! GAAGCATCACACCGCTGCTT+CGG - chr1_1:64301890-64301909 None:intergenic 55.0%
GACCACGAGCTCAAAGGTGA+AGG + chr1_1:64301915-64301934 Msa0035980:CDS 55.0%
GAACGATGAAGCCACCTAGC+AGG - chr1_1:64301969-64301988 None:intergenic 55.0%
TCCGGGAAAACAGCTGGAGT+CGG - chr1_1:64302074-64302093 None:intergenic 55.0%
!! GTACAGGGATTGGTTGGTGG+TGG + chr1_1:64302375-64302394 Msa0035980:intron 55.0%
!! TGGTTGGTGGTGGTGGTGTT+TGG + chr1_1:64302385-64302404 Msa0035980:intron 55.0%
!! GGTATAGGGGTGCTTGCTGA+TGG + chr1_1:64302786-64302805 Msa0035980:intron 55.0%
!! TAGGGGTGCTTGCTGATGGT+CGG + chr1_1:64302790-64302809 Msa0035980:intron 55.0%
GCTACTGGCATTTGAGCTGC+AGG + chr1_1:64303982-64304001 Msa0035980:three_prime_UTR 55.0%
GGCGCAGAGTACACCAAGCA+TGG - chr1_1:64301673-64301692 None:intergenic 60.0%
GATCCAAGGACGAGAGCGAG+TGG - chr1_1:64301694-64301713 None:intergenic 60.0%
!! AAAGGCGTGGTGGTGGTGGT+GGG - chr1_1:64301724-64301743 None:intergenic 60.0%
! AGAGACGAAAGGCGTGGTGG+TGG - chr1_1:64301731-64301750 None:intergenic 60.0%
! GAGAGAGACGAAAGGCGTGG+TGG - chr1_1:64301734-64301753 None:intergenic 60.0%
CGTGAGAGAGACGAAAGGCG+TGG - chr1_1:64301737-64301756 None:intergenic 60.0%
! CGCCGGTTGAGATTTGGAGG+TGG - chr1_1:64301815-64301834 None:intergenic 60.0%
! CACCGCCGGTTGAGATTTGG+AGG - chr1_1:64301818-64301837 None:intergenic 60.0%
CTCCAAATCTCAACCGGCGG+TGG + chr1_1:64301816-64301835 Msa0035980:CDS 60.0%
! CTCCACCGCCGGTTGAGATT+TGG - chr1_1:64301821-64301840 None:intergenic 60.0%
TATCGAAGCAGCTCCACCGC+CGG - chr1_1:64301832-64301851 None:intergenic 60.0%
GCGGTGGAGCTGCTTCGATA+TGG + chr1_1:64301832-64301851 Msa0035980:CDS 60.0%
CACGCGATTACTCCTTGCGG+CGG + chr1_1:64301855-64301874 Msa0035980:CDS 60.0%
ATTACTCCTTGCGGCGGCGA+TGG + chr1_1:64301861-64301880 Msa0035980:CDS 60.0%
! GGCGATGGATTCCGAAGCAG+CGG + chr1_1:64301876-64301895 Msa0035980:CDS 60.0%
CTTGGGATGCTCGTCCTGCT+AGG + chr1_1:64301952-64301971 Msa0035980:CDS 60.0%
CAACAATAACCGGCGGCGCA+AGG - chr1_1:64302028-64302047 None:intergenic 60.0%
AAAACAGCTGGAGTCGGCGG+CGG - chr1_1:64302068-64302087 None:intergenic 60.0%
GGGAAAACAGCTGGAGTCGG+CGG - chr1_1:64302071-64302090 None:intergenic 60.0%
!! GCCGACTCCAGCTGTTTTCC+CGG + chr1_1:64302070-64302089 Msa0035980:CDS 60.0%
CTCTCGTCCGGGAAAACAGC+TGG - chr1_1:64302080-64302099 None:intergenic 60.0%
TTTCCCGGACGAGAGTTCCG+AGG + chr1_1:64302085-64302104 Msa0035980:CDS 60.0%
TTCCCGGACGAGAGTTCCGA+GGG + chr1_1:64302086-64302105 Msa0035980:CDS 60.0%
!! CAGGGATTGGTTGGTGGTGG+TGG + chr1_1:64302378-64302397 Msa0035980:intron 60.0%
!! GAAAGGCGTGGTGGTGGTGG+TGG - chr1_1:64301725-64301744 None:intergenic 65.0%
!! GACGAAAGGCGTGGTGGTGG+TGG - chr1_1:64301728-64301747 None:intergenic 65.0%
TGCAGGCGGTGGAATGGTGG+CGG - chr1_1:64301791-64301810 None:intergenic 65.0%
GGTTGCAGGCGGTGGAATGG+TGG - chr1_1:64301794-64301813 None:intergenic 65.0%
GGTGGTTGCAGGCGGTGGAA+TGG - chr1_1:64301797-64301816 None:intergenic 65.0%
TTGGAGGTGGTTGCAGGCGG+TGG - chr1_1:64301802-64301821 None:intergenic 65.0%
GGGATGCTCGTCCTGCTAGG+TGG + chr1_1:64301955-64301974 Msa0035980:CDS 65.0%
GTCCCTCGGAACTCTCGTCC+GGG - chr1_1:64302091-64302110 None:intergenic 65.0%
CGTCCCTCGGAACTCTCGTC+CGG - chr1_1:64302092-64302111 None:intergenic 65.0%
GCGCCACTCGCTCTCGTCCT+TGG + chr1_1:64301688-64301707 Msa0035980:CDS 70.0%
!! AGGCGGTGGAATGGTGGCGG+CGG - chr1_1:64301788-64301807 None:intergenic 70.0%
CGGAATCCATCGCCGCCGCA+AGG - chr1_1:64301870-64301889 None:intergenic 70.0%
CGGCGGGCGGGGAAAGTGAG+AGG - chr1_1:64301771-64301790 None:intergenic 75.0%
!! TGGAATGGTGGCGGCGGGCG+GGG - chr1_1:64301782-64301801 None:intergenic 75.0%
!! GTGGAATGGTGGCGGCGGGC+GGG - chr1_1:64301783-64301802 None:intergenic 75.0%
!! GGTGGAATGGTGGCGGCGGG+CGG - chr1_1:64301784-64301803 None:intergenic 75.0%
!! GGCGGTGGAATGGTGGCGGC+GGG - chr1_1:64301787-64301806 None:intergenic 75.0%
CTGCGCTTGCCTTGCGCCGC+CGG + chr1_1:64302016-64302035 Msa0035980:CDS 75.0%
! TGGAGTCGGCGGCGGCGCCT+CGG - chr1_1:64302060-64302079 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 64301427 64304340 64301427 ID=Msa0035980;Name=Msa0035980
chr1_1 mRNA 64301427 64304340 64301427 ID=Msa0035980-mRNA-1;Parent=Msa0035980;Name=Msa0035980-mRNA-1;_AED=0.04;_eAED=0.04;_QI=245|1|1|1|0.75|0.6|5|503|305
chr1_1 exon 64301427 64302176 64301427 ID=Msa0035980-mRNA-1:exon:20587;Parent=Msa0035980-mRNA-1
chr1_1 exon 64302793 64302914 64302793 ID=Msa0035980-mRNA-1:exon:20588;Parent=Msa0035980-mRNA-1
chr1_1 exon 64303029 64303069 64303029 ID=Msa0035980-mRNA-1:exon:20589;Parent=Msa0035980-mRNA-1
chr1_1 exon 64303170 64303268 64303170 ID=Msa0035980-mRNA-1:exon:20590;Parent=Msa0035980-mRNA-1
chr1_1 exon 64303687 64304340 64303687 ID=Msa0035980-mRNA-1:exon:20591;Parent=Msa0035980-mRNA-1
chr1_1 five_prime_UTR 64301427 64301671 64301427 ID=Msa0035980-mRNA-1:five_prime_utr;Parent=Msa0035980-mRNA-1
chr1_1 CDS 64301672 64302176 64301672 ID=Msa0035980-mRNA-1:cds;Parent=Msa0035980-mRNA-1
chr1_1 CDS 64302793 64302914 64302793 ID=Msa0035980-mRNA-1:cds;Parent=Msa0035980-mRNA-1
chr1_1 CDS 64303029 64303069 64303029 ID=Msa0035980-mRNA-1:cds;Parent=Msa0035980-mRNA-1
chr1_1 CDS 64303170 64303268 64303170 ID=Msa0035980-mRNA-1:cds;Parent=Msa0035980-mRNA-1
chr1_1 CDS 64303687 64303837 64303687 ID=Msa0035980-mRNA-1:cds;Parent=Msa0035980-mRNA-1
chr1_1 three_prime_UTR 64303838 64304340 64303838 ID=Msa0035980-mRNA-1:three_prime_utr;Parent=Msa0035980-mRNA-1
Gene Sequence

>Msa0035980

ATGCTTGGTGTACTCTGCGCCACTCGCTCTCGTCCTTGGATCTTCTCTTCCCACCACCACCACCACGCCTTTCGTCTCTCTCACGCCACTGTCGCTCCTCTCACTTTCCCCGCCCGCCGCCACCATTCCACCGCCTGCAACCACCTCCAAATCTCAACCGGCGGTGGAGCTGCTTCGATATGGCACGCGATTACTCCTTGCGGCGGCGATGGATTCCGAAGCAGCGGTGTGATGCTTCATCATGACCACGAGCTCAAAGGTGAAGGATCGTGGAACGTTGCTTGGGATGCTCGTCCTGCTAGGTGGCTTCATCGTTCTGACTCAGCTTGGCTTCTTTTCGGTGTCTGCGCTTGCCTTGCGCCGCCGGTTATTGTTGACGTTGATACCGAGGCGCCGCCGCCGACTCCAGCTGTTTTCCCGGACGAGAGTTCCGAGGGACGTGAAGTGAAAGATGAGCTTTCCGATGAACGTGACGATGAACTCAACGCTGATTACAGAGTCACGGGGGTGCTTGCTGATGGTCGGTGTCTGTTCAGAGCAATAGCTCATGGAGCTTGCTTGAAAAATGGAGAGGAAGCGCCTAATGAAAGTCGACAGAGGGAACTTGCCGACGAATTAAGGGTTAAAGTTGCTGAAGAACTTTTGAACAGGCGCAAGGAAACAGAATGGTTTATAGAAGGGGATTTTGACACATATGTGACCAGAATTCAACAGACTTATGTTTGGGGAGGAGAGCCTGAGCTATTAATGGCTTCTCATGTTTTGAAGACTCCAATATATGTCTTCATGAGAGATGCAAGCTCCATGGATTTGGTAAATATAGCAAAATACGGTGAAGAATATATGAATGATGAGGAGATTTCCATCAATGTGCTATTTCATCGACATGGTCACTACGAACTATTGGAGACCTTATGA
Protein sequence

>Msa0035980

MLGVLCATRSRPWIFSSHHHHHAFRLSHATVAPLTFPARRHHSTACNHLQISTGGGAASIWHAITPCGGDGFRSSGVMLHHDHELKGEGSWNVAWDARPARWLHRSDSAWLLFGVCACLAPPVIVDVDTEAPPPTPAVFPDESSEGREVKDELSDERDDELNADYRVTGVLADGRCLFRAIAHGACLKNGEEAPNESRQRELADELRVKVAEELLNRRKETEWFIEGDFDTYVTRIQQTYVWGGEPELLMASHVLKTPIYVFMRDASSMDLVNIAKYGEEYMNDEEISINVLFHRHGHYELLETL*