AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0036060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036060 MtrunA17_Chr1g0198131 97.049 305 9 0 1 305 1 305 0.0 618
Msa0036060 MtrunA17_Chr1g0174241 46.411 209 90 4 102 304 135 327 2.79e-58 190
Msa0036060 MtrunA17_Chr3g0122741 49.007 151 73 2 153 303 2 148 8.37e-49 159
Msa0036060 MtrunA17_Chr1g0174221 47.945 146 73 2 159 303 91 234 8.85e-43 147
Msa0036060 MtrunA17_Chr4g0005721 35.294 153 83 5 165 302 77 228 5.84e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0036060 AT3G57810.2 58.571 140 56 1 163 302 166 303 1.16e-55 183
Msa0036060 AT3G57810.4 58.571 140 56 1 163 302 166 303 1.16e-55 183
Msa0036060 AT3G57810.3 58.571 140 56 1 163 302 166 303 1.16e-55 183
Msa0036060 AT2G38025.2 32.075 159 86 4 165 302 53 210 4.38e-15 73.2
Msa0036060 AT2G38025.1 32.075 159 86 4 165 302 76 233 7.91e-15 72.8
Msa0036060 AT3G57810.1 39.362 94 52 2 123 211 121 214 1.74e-14 71.6

Find 129 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACTTGCCGACGAATTA+AGG 0.213414 1_1:+64396415 Msa0036060:CDS
ATCATGCGGTATAAGTCTTC+TGG 0.238749 1_1:+64397423 Msa0036060:three_prime_UTR
CTTAATAATAAAACCCGTTA+TGG 0.281750 1_1:+64395118 Msa0036060:five_prime_UTR
CTCCACCGCCGGTTGAGATT+TGG 0.298384 1_1:-64395355 None:intergenic
AACATAAGTCTGTTGAATTC+TGG 0.316175 1_1:-64396731 None:intergenic
TGGAATGGTGGCGGCGGGCG+GGG 0.325826 1_1:-64395316 None:intergenic
TGAGATTTGGAGGTGGTTGC+AGG 0.327466 1_1:-64395342 None:intergenic
GGTGGAATGGTGGCGGCGGG+CGG 0.330689 1_1:-64395318 None:intergenic
AATTCAACAGACTTATGTTT+GGG 0.341402 1_1:+64396735 Msa0036060:CDS
TCTCTCATGAAGACATATAT+TGG 0.349253 1_1:-64397215 None:intergenic
GTGGAATGGTGGCGGCGGGC+GGG 0.352247 1_1:-64395317 None:intergenic
GCGCCACTCGCTCTCGTCCT+TGG 0.358149 1_1:+64395225 Msa0036060:CDS
AGATGCAAGCTCCATGGATT+TGG 0.364917 1_1:+64397235 Msa0036060:CDS
AGGAGAGCCTGAGCTATTAA+TGG 0.369529 1_1:+64396759 Msa0036060:CDS
AGAGAAGCGTGGGTGTTATA+AGG 0.369781 1_1:-64395022 None:intergenic
CTGCGCTTGCCTTGCGCCGC+CGG 0.373717 1_1:+64395553 Msa0036060:CDS
ACTGCAAAACAAAAGTTCAT+TGG 0.380213 1_1:-64397544 None:intergenic
GAAAGGCGTGGTGGTGGTGG+TGG 0.384833 1_1:-64395259 None:intergenic
ACATGGTCACTACGAACTAT+TGG 0.387672 1_1:+64397328 Msa0036060:CDS
TTCCATTCCAAGAAAAGAAA+AGG 0.389727 1_1:+64394988 Msa0036060:five_prime_UTR
TGGAGTCGGCGGCGCCGCCT+CGG 0.392242 1_1:-64395594 None:intergenic
GAATTCAACAGACTTATGTT+TGG 0.396680 1_1:+64396734 Msa0036060:CDS
GCTACTGGCATTTGAGCTGC+AGG 0.412012 1_1:+64397506 Msa0036060:three_prime_UTR
CAAAAGTGGACATTGTTGTT+TGG 0.413224 1_1:+64394965 Msa0036060:five_prime_UTR
TCGATGAAATAGCACATTGA+TGG 0.414542 1_1:-64397306 None:intergenic
GGCGGTGGAATGGTGGCGGC+GGG 0.418826 1_1:-64395321 None:intergenic
GGAAGGTTTAGGTGAGTTGG+TGG 0.420220 1_1:+64397587 Msa0036060:three_prime_UTR
ATCTTTCACTTCACGTCCCT+CGG 0.423234 1_1:-64395639 None:intergenic
GAAGCATCACACCGCTGCTT+CGG 0.424353 1_1:-64395424 None:intergenic
TCCCTCTGTCGACTTTCATT+AGG 0.424371 1_1:-64396396 None:intergenic
GCACTGTGTATGTGAGTGTA+TGG 0.425283 1_1:-64395163 None:intergenic
CTCTCGTCCGGGAAAACAGC+TGG 0.428780 1_1:-64395614 None:intergenic
TTGGAGGTGGTTGCAGGCGG+TGG 0.429035 1_1:-64395336 None:intergenic
AATGGAAGGTTTAGGTGAGT+TGG 0.429406 1_1:+64397584 Msa0036060:three_prime_UTR
TGCTCGATTCTTGATACTCA+TGG 0.435244 1_1:+64397643 Msa0036060:three_prime_UTR
ATTACTCCTTGCGGCGGCGA+TGG 0.436846 1_1:+64395398 Msa0036060:CDS
TTAATAATAAAACCCGTTAT+GGG 0.437129 1_1:+64395119 Msa0036060:five_prime_UTR
GGAACTTGCCGACGAATTAA+GGG 0.438594 1_1:+64396416 Msa0036060:CDS
CGTCCCTCGGAACTCTCGTC+CGG 0.439061 1_1:-64395626 None:intergenic
TTATCCTACGCACCCATAAC+GGG 0.440177 1_1:-64395131 None:intergenic
GAGTTCATCGTCACGTTCAT+CGG 0.445917 1_1:-64395669 None:intergenic
AAGGATCGTGGAACGTTGCT+TGG 0.447140 1_1:+64395471 Msa0036060:CDS
TTCATCGTTCTGACTCAGCT+TGG 0.448002 1_1:+64395516 Msa0036060:CDS
TGCTATATTTACCAAATCCA+TGG 0.450384 1_1:-64397246 None:intergenic
TGTGTCTATAGGTTTATAGA+AGG 0.457572 1_1:+64396688 Msa0036060:intron
TCATGCGGTATAAGTCTTCT+GGG 0.458739 1_1:+64397424 Msa0036060:three_prime_UTR
GCGGTGGAGCTGCTTCGATA+TGG 0.467969 1_1:+64395369 Msa0036060:CDS
GACGAAAGGCGTGGTGGTGG+TGG 0.470943 1_1:-64395262 None:intergenic
AGGCGGTGGAATGGTGGCGG+CGG 0.476240 1_1:-64395322 None:intergenic
CACCGCCGGTTGAGATTTGG+AGG 0.479044 1_1:-64395352 None:intergenic
GTATCAACGTCAACAATAAC+CGG 0.479760 1_1:-64395572 None:intergenic
CTTGGGATGCTCGTCCTGCT+AGG 0.480235 1_1:+64395489 Msa0036060:CDS
TACTTTAACCCTTAATTCGT+CGG 0.484358 1_1:-64396424 None:intergenic
ATATGCAATCAATGTACAAA+AGG 0.492528 1_1:-64397811 None:intergenic
AAAGGCGTGGTGGTGGTGGT+GGG 0.493789 1_1:-64395258 None:intergenic
CTCCAAATCTCAACCGGCGG+TGG 0.496363 1_1:+64395353 Msa0036060:CDS
GGTGGTTGCAGGCGGTGGAA+TGG 0.499927 1_1:-64395331 None:intergenic
GCCACTCACTCATCCATGCT+TGG 0.503058 1_1:+64395194 Msa0036060:exon
ATCCTTCACCTTTGAGCTCG+TGG 0.505031 1_1:-64395454 None:intergenic
TTGGTAAATATAGCAAAATA+CGG 0.508333 1_1:+64397254 Msa0036060:CDS
AGAGAAGAGAGCAATAAGAA+TGG 0.511242 1_1:-64395071 None:intergenic
CGCCGGTTGAGATTTGGAGG+TGG 0.513387 1_1:-64395349 None:intergenic
CACTGTGTATGTGAGTGTAT+GGG 0.514314 1_1:-64395162 None:intergenic
TGAGAAGCCATTAATAGCTC+AGG 0.515628 1_1:-64396766 None:intergenic
AACCACCTCCAAATCTCAAC+CGG 0.518771 1_1:+64395347 Msa0036060:CDS
GTTATCCTACGCACCCATAA+CGG 0.524419 1_1:-64395132 None:intergenic
AGGATCGTGGAACGTTGCTT+GGG 0.525778 1_1:+64395472 Msa0036060:CDS
CTACTGGCATTTGAGCTGCA+GGG 0.526098 1_1:+64397507 Msa0036060:three_prime_UTR
TGCAGGCGGTGGAATGGTGG+CGG 0.528482 1_1:-64395325 None:intergenic
CGGCGGGCGGGGAAAGTGAG+AGG 0.534229 1_1:-64395305 None:intergenic
GCTCGATTCTTGATACTCAT+GGG 0.538567 1_1:+64397644 Msa0036060:three_prime_UTR
GTCCCTCGGAACTCTCGTCC+GGG 0.539858 1_1:-64395625 None:intergenic
CTGTTCAGAGCAATAGCTCA+TGG 0.539965 1_1:+64396345 Msa0036060:CDS
CAAAAGGAGCCAACGAATGT+TGG 0.540663 1_1:-64397795 None:intergenic
TTAAGTGAACCAACATTCGT+TGG 0.542574 1_1:+64397786 Msa0036060:three_prime_UTR
GTGTCTATAGGTTTATAGAA+GGG 0.543445 1_1:+64396689 Msa0036060:intron
TGTCTATAGGTTTATAGAAG+GGG 0.544260 1_1:+64396690 Msa0036060:intron
GAACGATGAAGCCACCTAGC+AGG 0.545883 1_1:-64395503 None:intergenic
GGTTGCAGGCGGTGGAATGG+TGG 0.547561 1_1:-64395328 None:intergenic
TAGGGGTGCTTGCTGATGGT+CGG 0.550798 1_1:+64396319 Msa0036060:intron
TCCATTCCAAGAAAAGAAAA+GGG 0.553262 1_1:+64394989 Msa0036060:five_prime_UTR
TGTCAGCATAAGAACTTCGT+TGG 0.553366 1_1:-64397724 None:intergenic
CAACAATAACCGGCGGCGCA+AGG 0.554275 1_1:-64395562 None:intergenic
CGGAATCCATCGCCGCCGCA+AGG 0.559044 1_1:-64395404 None:intergenic
GGCGCAGAGTACACCAAGCA+TGG 0.563817 1_1:-64395207 None:intergenic
AGCTCAAAGGTGAAGGATCG+TGG 0.569548 1_1:+64395459 Msa0036060:CDS
AACGCTGATTACAGAGTCAC+GGG 0.570421 1_1:+64395692 Msa0036060:CDS
CATGAGAGATGCAAGCTCCA+TGG 0.570844 1_1:+64397229 Msa0036060:CDS
GAAGAGAAAGAGAGAAGCGT+GGG 0.573463 1_1:-64395032 None:intergenic
TCCGGGAAAACAGCTGGAGT+CGG 0.574081 1_1:-64395608 None:intergenic
CAACAGACTTATGTTTGGGG+AGG 0.576052 1_1:+64396739 Msa0036060:CDS
GACCACGAGCTCAAAGGTGA+AGG 0.577751 1_1:+64395452 Msa0036060:CDS
AAAACCCGTTATGGGTGCGT+AGG 0.577803 1_1:+64395127 Msa0036060:five_prime_UTR
GATCCAAGGACGAGAGCGAG+TGG 0.579496 1_1:-64395228 None:intergenic
GGTATAGGGGTGCTTGCTGA+TGG 0.584649 1_1:+64396315 Msa0036060:intron
TATCGAAGCAGCTCCACCGC+CGG 0.586713 1_1:-64395366 None:intergenic
CATCATGACCACGAGCTCAA+AGG 0.587256 1_1:+64395446 Msa0036060:CDS
TTCCCGGACGAGAGTTCCGA+GGG 0.588950 1_1:+64395623 Msa0036060:CDS
AGAGACGAAAGGCGTGGTGG+TGG 0.589008 1_1:-64395265 None:intergenic
AGTCTTAAAGAACACTTGCA+TGG 0.592866 1_1:+64397683 Msa0036060:three_prime_UTR
ATTTAGTTTCTTTAATCATG+CGG 0.595658 1_1:+64397409 Msa0036060:three_prime_UTR
GAAAGTGAGAGGAGCGACAG+TGG 0.606183 1_1:-64395294 None:intergenic
CAACGCTGATTACAGAGTCA+CGG 0.607136 1_1:+64395691 Msa0036060:CDS
CACGCGATTACTCCTTGCGG+CGG 0.611520 1_1:+64395392 Msa0036060:CDS
TTTCCCGGACGAGAGTTCCG+AGG 0.612131 1_1:+64395622 Msa0036060:CDS
GCCTAATGAAAGTCGACAGA+GGG 0.612158 1_1:+64396395 Msa0036060:CDS
CGAAGAGAAAGAGAGAAGCG+TGG 0.621048 1_1:-64395033 None:intergenic
GGCGATGGATTCCGAAGCAG+CGG 0.621773 1_1:+64395413 Msa0036060:CDS
TCCAAGAAAAGAAAAGGGTG+AGG 0.622110 1_1:+64394994 Msa0036060:five_prime_UTR
TATTGTTGACGTTGATACCG+AGG 0.628764 1_1:+64395577 Msa0036060:CDS
GGGAAAACAGCTGGAGTCGG+CGG 0.628808 1_1:-64395605 None:intergenic
TGAAGAATATATGAATGATG+AGG 0.630448 1_1:+64397277 Msa0036060:CDS
ACCAAGCATGGATGAGTGAG+TGG 0.631102 1_1:-64395195 None:intergenic
GATTTGGAGGTGGTTGCAGG+CGG 0.637060 1_1:-64395339 None:intergenic
ACTGTGTATGTGAGTGTATG+GGG 0.640581 1_1:-64395161 None:intergenic
TGGTGGGAAGAGAAGATCCA+AGG 0.650314 1_1:-64395242 None:intergenic
CGCCTAATGAAAGTCGACAG+AGG 0.650904 1_1:+64396394 Msa0036060:CDS
CACCTCCAAATCTCAACCGG+CGG 0.653859 1_1:+64395350 Msa0036060:CDS
GGGATGCTCGTCCTGCTAGG+TGG 0.655002 1_1:+64395492 Msa0036060:CDS
ACAGGCGCAAGGAAACAGAA+TGG 0.661069 1_1:+64396577 Msa0036060:CDS
ATTCAACAGACTTATGTTTG+GGG 0.663997 1_1:+64396736 Msa0036060:CDS
AATGTGCTATTTCATCGACA+TGG 0.668256 1_1:+64397311 Msa0036060:CDS
GAAAAGAAAAGGGTGAGGTG+AGG 0.669407 1_1:+64394999 Msa0036060:five_prime_UTR
GAGAGAGACGAAAGGCGTGG+TGG 0.678901 1_1:-64395268 None:intergenic
CGTGAGAGAGACGAAAGGCG+TGG 0.686067 1_1:-64395271 None:intergenic
AGTGGCGTGAGAGAGACGAA+AGG 0.697149 1_1:-64395276 None:intergenic
TGGCACGCGATTACTCCTTG+CGG 0.725561 1_1:+64395389 Msa0036060:CDS
TGTTGACGTTGATACCGAGG+CGG 0.731095 1_1:+64395580 Msa0036060:CDS
TCAACGTCAACAATAACCGG+CGG 0.746562 1_1:-64395569 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAACATAACAAAAATT+GGG - chr1_1:64396535-64396554 None:intergenic 10.0%
!! AAATAAAACATAACAAAAAT+TGG - chr1_1:64396536-64396555 None:intergenic 10.0%
!!! ATTTTTGTTATGTTTTATTT+AGG + chr1_1:64396536-64396555 Msa0036060:intron 10.0%
!!! TTATTTTACTTTTATTTACA+TGG + chr1_1:64396644-64396663 Msa0036060:intron 10.0%
!!! TTTTTGTATTTTCTTTTTAA+TGG + chr1_1:64397566-64397585 Msa0036060:three_prime_UTR 10.0%
!!! ATTTCATTTTTTGTCTTTAA+TGG + chr1_1:64395872-64395891 Msa0036060:intron 15.0%
!!! TATTTTTTTCATATAAGTAC+AGG + chr1_1:64395896-64395915 Msa0036060:intron 15.0%
!!! ATTTTTTTCATATAAGTACA+GGG + chr1_1:64395897-64395916 Msa0036060:intron 15.0%
!!! TTTTTATTTTTTTTGAAAGG+AGG + chr1_1:64397615-64397634 Msa0036060:three_prime_UTR 15.0%
!! TTAATAATAAAACCCGTTAT+GGG + chr1_1:64395119-64395138 Msa0036060:five_prime_UTR 20.0%
!!! TTATTGTTCAGTTATTTTGA+TGG + chr1_1:64396223-64396242 Msa0036060:intron 20.0%
!! AAATTCATTGAAGGAAAAAA+AGG - chr1_1:64396507-64396526 None:intergenic 20.0%
!! TTGGTAAATATAGCAAAATA+CGG + chr1_1:64397254-64397273 Msa0036060:CDS 20.0%
!!! ATTTAGTTTCTTTAATCATG+CGG + chr1_1:64397409-64397428 Msa0036060:three_prime_UTR 20.0%
!!! TGTATTTTCTTTTTAATGGA+AGG + chr1_1:64397570-64397589 Msa0036060:three_prime_UTR 20.0%
!!! TTCTCTTCGTTCTTTTTTAT+TGG + chr1_1:64395047-64395066 Msa0036060:five_prime_UTR 25.0%
!!! ACGGGTTTTATTATTAAGAT+TGG - chr1_1:64395116-64395135 None:intergenic 25.0%
! CTTAATAATAAAACCCGTTA+TGG + chr1_1:64395118-64395137 Msa0036060:five_prime_UTR 25.0%
! AACAAGAACTATTGTATCAT+TGG - chr1_1:64396137-64396156 None:intergenic 25.0%
! TATAAGTTTGTATCACTGAA+TGG + chr1_1:64396294-64396313 Msa0036060:intron 25.0%
! GGAAACTACATACAATTATT+TGG + chr1_1:64396618-64396637 Msa0036060:intron 25.0%
! AATTCAACAGACTTATGTTT+GGG + chr1_1:64396735-64396754 Msa0036060:CDS 25.0%
! AATATCATATCCTACAACAA+CGG + chr1_1:64396940-64396959 Msa0036060:intron 25.0%
! TAATGAAAGATGATAATCGA+TGG + chr1_1:64397007-64397026 Msa0036060:intron 25.0%
! AATCGATGGATATTAATCTA+CGG + chr1_1:64397021-64397040 Msa0036060:intron 25.0%
! TAAGTTATGAAGACAATGTT+AGG - chr1_1:64397187-64397206 None:intergenic 25.0%
! TGAAGAATATATGAATGATG+AGG + chr1_1:64397277-64397296 Msa0036060:CDS 25.0%
!!! GTTATTTTTGAGACAACTTT+TGG - chr1_1:64397365-64397384 None:intergenic 25.0%
!!! AAGAGCTTTTTTTGTCATTT+GGG + chr1_1:64397452-64397471 Msa0036060:three_prime_UTR 25.0%
!!! TTTAGTAGTTTTTTTGCTAC+TGG + chr1_1:64397491-64397510 Msa0036060:three_prime_UTR 25.0%
!! TTCTTTTTAATGGAAGGTTT+AGG + chr1_1:64397576-64397595 Msa0036060:three_prime_UTR 25.0%
! ATATGCAATCAATGTACAAA+AGG - chr1_1:64397814-64397833 None:intergenic 25.0%
TTCCATTCCAAGAAAAGAAA+AGG + chr1_1:64394988-64395007 Msa0036060:five_prime_UTR 30.0%
TCCATTCCAAGAAAAGAAAA+GGG + chr1_1:64394989-64395008 Msa0036060:five_prime_UTR 30.0%
TTTCATATAAGTACAGGGAT+TGG + chr1_1:64395902-64395921 Msa0036060:intron 30.0%
ACTTGTTCAATATGAAGTGT+CGG + chr1_1:64396174-64396193 Msa0036060:intron 30.0%
AACATGAGTTAGAGATTATG+TGG + chr1_1:64396264-64396283 Msa0036060:intron 30.0%
ACATGAGTTAGAGATTATGT+GGG + chr1_1:64396265-64396284 Msa0036060:intron 30.0%
TTTGTATCACTGAATGGTAT+AGG + chr1_1:64396300-64396319 Msa0036060:intron 30.0%
TTGTATCACTGAATGGTATA+GGG + chr1_1:64396301-64396320 Msa0036060:intron 30.0%
TACTTTAACCCTTAATTCGT+CGG - chr1_1:64396427-64396446 None:intergenic 30.0%
!! TCTTGCTAGAACTTTTGAAA+CGG + chr1_1:64396453-64396472 Msa0036060:intron 30.0%
TGGGTAACAAAATTCATTGA+AGG - chr1_1:64396516-64396535 None:intergenic 30.0%
!! GTTATATTTGCTGTGTCTAT+AGG + chr1_1:64396677-64396696 Msa0036060:intron 30.0%
TGTGTCTATAGGTTTATAGA+AGG + chr1_1:64396688-64396707 Msa0036060:intron 30.0%
GTGTCTATAGGTTTATAGAA+GGG + chr1_1:64396689-64396708 Msa0036060:intron 30.0%
TGTCTATAGGTTTATAGAAG+GGG + chr1_1:64396690-64396709 Msa0036060:intron 30.0%
AACATAAGTCTGTTGAATTC+TGG - chr1_1:64396734-64396753 None:intergenic 30.0%
GAATTCAACAGACTTATGTT+TGG + chr1_1:64396734-64396753 Msa0036060:CDS 30.0%
ATTCAACAGACTTATGTTTG+GGG + chr1_1:64396736-64396755 Msa0036060:CDS 30.0%
ATCGATGGATATTAATCTAC+GGG + chr1_1:64397022-64397041 Msa0036060:intron 30.0%
! TTCATTTTGTGGTAAATCGT+TGG + chr1_1:64397096-64397115 Msa0036060:intron 30.0%
! TCATTTTGTGGTAAATCGTT+GGG + chr1_1:64397097-64397116 Msa0036060:intron 30.0%
CATTGTCTTCATAACTTACA+AGG + chr1_1:64397189-64397208 Msa0036060:intron 30.0%
TCTCTCATGAAGACATATAT+TGG - chr1_1:64397218-64397237 None:intergenic 30.0%
TGCTATATTTACCAAATCCA+TGG - chr1_1:64397249-64397268 None:intergenic 30.0%
!!! GAAGAGCTTTTTTTGTCATT+TGG + chr1_1:64397451-64397470 Msa0036060:three_prime_UTR 30.0%
!!! AGAGCTTTTTTTGTCATTTG+GGG + chr1_1:64397453-64397472 Msa0036060:three_prime_UTR 30.0%
ACTGCAAAACAAAAGTTCAT+TGG - chr1_1:64397547-64397566 None:intergenic 30.0%
! CAAAAGTGGACATTGTTGTT+TGG + chr1_1:64394965-64394984 Msa0036060:five_prime_UTR 35.0%
! ACCCTTTTCTTTTCTTGGAA+TGG - chr1_1:64394993-64395012 None:intergenic 35.0%
AGAGAAGAGAGCAATAAGAA+TGG - chr1_1:64395074-64395093 None:intergenic 35.0%
GTATCAACGTCAACAATAAC+CGG - chr1_1:64395575-64395594 None:intergenic 35.0%
! ATATAAGTACAGGGATTGGT+TGG + chr1_1:64395906-64395925 Msa0036060:intron 35.0%
TTACAACTACGAAAGACATC+AGG + chr1_1:64396060-64396079 Msa0036060:intron 35.0%
ATTGAACAAGTGTCTTGAGT+TGG - chr1_1:64396165-64396184 None:intergenic 35.0%
TGTATCACTGAATGGTATAG+GGG + chr1_1:64396302-64396321 Msa0036060:intron 35.0%
AAGGATGTTGACAAAGAAGA+AGG - chr1_1:64396488-64396507 None:intergenic 35.0%
! TTGCTGAAGAACTTTTGAAC+AGG + chr1_1:64396559-64396578 Msa0036060:CDS 35.0%
ACAAGTTAAACCGTTGTTGT+AGG - chr1_1:64396953-64396972 None:intergenic 35.0%
!! AACAACGGTTTAACTTGTTG+TGG + chr1_1:64396955-64396974 Msa0036060:intron 35.0%
! CAAATTTCCGCTTCATTTTG+TGG + chr1_1:64397085-64397104 Msa0036060:intron 35.0%
CGATTTACCACAAAATGAAG+CGG - chr1_1:64397095-64397114 None:intergenic 35.0%
GAAGACAATGTTAGGTACAA+CGG - chr1_1:64397179-64397198 None:intergenic 35.0%
TCGATGAAATAGCACATTGA+TGG - chr1_1:64397309-64397328 None:intergenic 35.0%
!! AATGTGCTATTTCATCGACA+TGG + chr1_1:64397311-64397330 Msa0036060:CDS 35.0%
! TGAGACAACTTTTGGTCATA+AGG - chr1_1:64397357-64397376 None:intergenic 35.0%
AGTCTTAAAGAACACTTGCA+TGG + chr1_1:64397683-64397702 Msa0036060:three_prime_UTR 35.0%
TTAAGTGAACCAACATTCGT+TGG + chr1_1:64397786-64397805 Msa0036060:three_prime_UTR 35.0%
TCCAAGAAAAGAAAAGGGTG+AGG + chr1_1:64394994-64395013 Msa0036060:five_prime_UTR 40.0%
! ACCTCACCCTTTTCTTTTCT+TGG - chr1_1:64394998-64395017 None:intergenic 40.0%
ACTGTGTATGTGAGTGTATG+GGG - chr1_1:64395164-64395183 None:intergenic 40.0%
CACTGTGTATGTGAGTGTAT+GGG - chr1_1:64395165-64395184 None:intergenic 40.0%
TATTGTTGACGTTGATACCG+AGG + chr1_1:64395577-64395596 Msa0036060:CDS 40.0%
GTTTGTGACCAAGTGTTTGA+TGG + chr1_1:64396020-64396039 Msa0036060:intron 40.0%
GTGACAAACCATCAAACACT+TGG - chr1_1:64396031-64396050 None:intergenic 40.0%
! CATGGAGCTTGCTTGAAAAA+TGG + chr1_1:64396363-64396382 Msa0036060:CDS 40.0%
! AGCTTGCTTGAAAAATGGAG+AGG + chr1_1:64396368-64396387 Msa0036060:CDS 40.0%
!!! CTAGAACTTTTGAAACGGAG+AGG + chr1_1:64396458-64396477 Msa0036060:intron 40.0%
! TGGTGAGCTTTGTCATTTGA+AGG + chr1_1:64396597-64396616 Msa0036060:intron 40.0%
TGAGAAGCCATTAATAGCTC+AGG - chr1_1:64396769-64396788 None:intergenic 40.0%
CAATGTTAGGTACAACGGAA+TGG - chr1_1:64397174-64397193 None:intergenic 40.0%
ACATGGTCACTACGAACTAT+TGG + chr1_1:64397328-64397347 Msa0036060:CDS 40.0%
ATCATGCGGTATAAGTCTTC+TGG + chr1_1:64397423-64397442 Msa0036060:three_prime_UTR 40.0%
TCATGCGGTATAAGTCTTCT+GGG + chr1_1:64397424-64397443 Msa0036060:three_prime_UTR 40.0%
AATGGAAGGTTTAGGTGAGT+TGG + chr1_1:64397584-64397603 Msa0036060:three_prime_UTR 40.0%
TGCTCGATTCTTGATACTCA+TGG + chr1_1:64397643-64397662 Msa0036060:three_prime_UTR 40.0%
GCTCGATTCTTGATACTCAT+GGG + chr1_1:64397644-64397663 Msa0036060:three_prime_UTR 40.0%
TGTCAGCATAAGAACTTCGT+TGG - chr1_1:64397727-64397746 None:intergenic 40.0%
GAAAAGAAAAGGGTGAGGTG+AGG + chr1_1:64394999-64395018 Msa0036060:five_prime_UTR 45.0%
! AGAGAAGCGTGGGTGTTATA+AGG - chr1_1:64395025-64395044 None:intergenic 45.0%
! GAAGAGAAAGAGAGAAGCGT+GGG - chr1_1:64395035-64395054 None:intergenic 45.0%
TTATCCTACGCACCCATAAC+GGG - chr1_1:64395134-64395153 None:intergenic 45.0%
GTTATCCTACGCACCCATAA+CGG - chr1_1:64395135-64395154 None:intergenic 45.0%
GCACTGTGTATGTGAGTGTA+TGG - chr1_1:64395166-64395185 None:intergenic 45.0%
AACCACCTCCAAATCTCAAC+CGG + chr1_1:64395347-64395366 Msa0036060:CDS 45.0%
TTCATCGTTCTGACTCAGCT+TGG + chr1_1:64395516-64395535 Msa0036060:CDS 45.0%
! GACTCAGCTTGGCTTCTTTT+CGG + chr1_1:64395527-64395546 Msa0036060:CDS 45.0%
TCAACGTCAACAATAACCGG+CGG - chr1_1:64395572-64395591 None:intergenic 45.0%
ATCTTTCACTTCACGTCCCT+CGG - chr1_1:64395642-64395661 None:intergenic 45.0%
GAGTTCATCGTCACGTTCAT+CGG - chr1_1:64395672-64395691 None:intergenic 45.0%
! CAACGCTGATTACAGAGTCA+CGG + chr1_1:64395691-64395710 Msa0036060:CDS 45.0%
! AACGCTGATTACAGAGTCAC+GGG + chr1_1:64395692-64395711 Msa0036060:CDS 45.0%
!! TAAGTACAGGGATTGGTTGG+TGG + chr1_1:64395909-64395928 Msa0036060:intron 45.0%
CTGTTCAGAGCAATAGCTCA+TGG + chr1_1:64396345-64396364 Msa0036060:CDS 45.0%
GCCTAATGAAAGTCGACAGA+GGG + chr1_1:64396395-64396414 Msa0036060:CDS 45.0%
!! TCCCTCTGTCGACTTTCATT+AGG - chr1_1:64396399-64396418 None:intergenic 45.0%
GGAACTTGCCGACGAATTAA+GGG + chr1_1:64396416-64396435 Msa0036060:CDS 45.0%
! AGAACTTTTGAACAGGCGCA+AGG + chr1_1:64396566-64396585 Msa0036060:CDS 45.0%
CAACAGACTTATGTTTGGGG+AGG + chr1_1:64396739-64396758 Msa0036060:CDS 45.0%
AGGAGAGCCTGAGCTATTAA+TGG + chr1_1:64396759-64396778 Msa0036060:CDS 45.0%
TGTGGTAAATCGTTGGGACT+TGG + chr1_1:64397103-64397122 Msa0036060:intron 45.0%
GTGGTAAATCGTTGGGACTT+GGG + chr1_1:64397104-64397123 Msa0036060:intron 45.0%
AGATGCAAGCTCCATGGATT+TGG + chr1_1:64397235-64397254 Msa0036060:CDS 45.0%
CAAAAGGAGCCAACGAATGT+TGG - chr1_1:64397798-64397817 None:intergenic 45.0%
!!! TTTTTTTTATTTTTTTTGAA+AGG + chr1_1:64397612-64397631 Msa0036060:three_prime_UTR 5.0%
CGAAGAGAAAGAGAGAAGCG+TGG - chr1_1:64395036-64395055 None:intergenic 50.0%
AAAACCCGTTATGGGTGCGT+AGG + chr1_1:64395127-64395146 Msa0036060:five_prime_UTR 50.0%
ACCAAGCATGGATGAGTGAG+TGG - chr1_1:64395198-64395217 None:intergenic 50.0%
TGGTGGGAAGAGAAGATCCA+AGG - chr1_1:64395245-64395264 None:intergenic 50.0%
TGAGATTTGGAGGTGGTTGC+AGG - chr1_1:64395345-64395364 None:intergenic 50.0%
CATCATGACCACGAGCTCAA+AGG + chr1_1:64395446-64395465 Msa0036060:CDS 50.0%
ATCCTTCACCTTTGAGCTCG+TGG - chr1_1:64395457-64395476 None:intergenic 50.0%
AGCTCAAAGGTGAAGGATCG+TGG + chr1_1:64395459-64395478 Msa0036060:CDS 50.0%
AAGGATCGTGGAACGTTGCT+TGG + chr1_1:64395471-64395490 Msa0036060:CDS 50.0%
AGGATCGTGGAACGTTGCTT+GGG + chr1_1:64395472-64395491 Msa0036060:CDS 50.0%
TGTTGACGTTGATACCGAGG+CGG + chr1_1:64395580-64395599 Msa0036060:CDS 50.0%
CGCCTAATGAAAGTCGACAG+AGG + chr1_1:64396394-64396413 Msa0036060:CDS 50.0%
GGGAACTTGCCGACGAATTA+AGG + chr1_1:64396415-64396434 Msa0036060:CDS 50.0%
ACAGGCGCAAGGAAACAGAA+TGG + chr1_1:64396577-64396596 Msa0036060:CDS 50.0%
CATGAGAGATGCAAGCTCCA+TGG + chr1_1:64397229-64397248 Msa0036060:CDS 50.0%
CTACTGGCATTTGAGCTGCA+GGG + chr1_1:64397507-64397526 Msa0036060:three_prime_UTR 50.0%
! GGAAGGTTTAGGTGAGTTGG+TGG + chr1_1:64397587-64397606 Msa0036060:three_prime_UTR 50.0%
GCCACTCACTCATCCATGCT+TGG + chr1_1:64395194-64395213 Msa0036060:exon 55.0%
AGTGGCGTGAGAGAGACGAA+AGG - chr1_1:64395279-64395298 None:intergenic 55.0%
GAAAGTGAGAGGAGCGACAG+TGG - chr1_1:64395297-64395316 None:intergenic 55.0%
GATTTGGAGGTGGTTGCAGG+CGG - chr1_1:64395342-64395361 None:intergenic 55.0%
CACCTCCAAATCTCAACCGG+CGG + chr1_1:64395350-64395369 Msa0036060:CDS 55.0%
TGGCACGCGATTACTCCTTG+CGG + chr1_1:64395389-64395408 Msa0036060:CDS 55.0%
! GAAGCATCACACCGCTGCTT+CGG - chr1_1:64395427-64395446 None:intergenic 55.0%
GACCACGAGCTCAAAGGTGA+AGG + chr1_1:64395452-64395471 Msa0036060:CDS 55.0%
GAACGATGAAGCCACCTAGC+AGG - chr1_1:64395506-64395525 None:intergenic 55.0%
TCCGGGAAAACAGCTGGAGT+CGG - chr1_1:64395611-64395630 None:intergenic 55.0%
!! GTACAGGGATTGGTTGGTGG+TGG + chr1_1:64395912-64395931 Msa0036060:intron 55.0%
!! TGGTTGGTGGTGGTGGTGTT+TGG + chr1_1:64395922-64395941 Msa0036060:intron 55.0%
!! GGTATAGGGGTGCTTGCTGA+TGG + chr1_1:64396315-64396334 Msa0036060:intron 55.0%
!! TAGGGGTGCTTGCTGATGGT+CGG + chr1_1:64396319-64396338 Msa0036060:intron 55.0%
GCTACTGGCATTTGAGCTGC+AGG + chr1_1:64397506-64397525 Msa0036060:three_prime_UTR 55.0%
GGCGCAGAGTACACCAAGCA+TGG - chr1_1:64395210-64395229 None:intergenic 60.0%
GATCCAAGGACGAGAGCGAG+TGG - chr1_1:64395231-64395250 None:intergenic 60.0%
!! AAAGGCGTGGTGGTGGTGGT+GGG - chr1_1:64395261-64395280 None:intergenic 60.0%
! AGAGACGAAAGGCGTGGTGG+TGG - chr1_1:64395268-64395287 None:intergenic 60.0%
! GAGAGAGACGAAAGGCGTGG+TGG - chr1_1:64395271-64395290 None:intergenic 60.0%
CGTGAGAGAGACGAAAGGCG+TGG - chr1_1:64395274-64395293 None:intergenic 60.0%
! CGCCGGTTGAGATTTGGAGG+TGG - chr1_1:64395352-64395371 None:intergenic 60.0%
! CACCGCCGGTTGAGATTTGG+AGG - chr1_1:64395355-64395374 None:intergenic 60.0%
CTCCAAATCTCAACCGGCGG+TGG + chr1_1:64395353-64395372 Msa0036060:CDS 60.0%
! CTCCACCGCCGGTTGAGATT+TGG - chr1_1:64395358-64395377 None:intergenic 60.0%
TATCGAAGCAGCTCCACCGC+CGG - chr1_1:64395369-64395388 None:intergenic 60.0%
GCGGTGGAGCTGCTTCGATA+TGG + chr1_1:64395369-64395388 Msa0036060:CDS 60.0%
CACGCGATTACTCCTTGCGG+CGG + chr1_1:64395392-64395411 Msa0036060:CDS 60.0%
ATTACTCCTTGCGGCGGCGA+TGG + chr1_1:64395398-64395417 Msa0036060:CDS 60.0%
! GGCGATGGATTCCGAAGCAG+CGG + chr1_1:64395413-64395432 Msa0036060:CDS 60.0%
CTTGGGATGCTCGTCCTGCT+AGG + chr1_1:64395489-64395508 Msa0036060:CDS 60.0%
CAACAATAACCGGCGGCGCA+AGG - chr1_1:64395565-64395584 None:intergenic 60.0%
GGGAAAACAGCTGGAGTCGG+CGG - chr1_1:64395608-64395627 None:intergenic 60.0%
!! GCCGACTCCAGCTGTTTTCC+CGG + chr1_1:64395607-64395626 Msa0036060:CDS 60.0%
CTCTCGTCCGGGAAAACAGC+TGG - chr1_1:64395617-64395636 None:intergenic 60.0%
TTTCCCGGACGAGAGTTCCG+AGG + chr1_1:64395622-64395641 Msa0036060:CDS 60.0%
TTCCCGGACGAGAGTTCCGA+GGG + chr1_1:64395623-64395642 Msa0036060:CDS 60.0%
!! CAGGGATTGGTTGGTGGTGG+TGG + chr1_1:64395915-64395934 Msa0036060:intron 60.0%
!! GAAAGGCGTGGTGGTGGTGG+TGG - chr1_1:64395262-64395281 None:intergenic 65.0%
!! GACGAAAGGCGTGGTGGTGG+TGG - chr1_1:64395265-64395284 None:intergenic 65.0%
TGCAGGCGGTGGAATGGTGG+CGG - chr1_1:64395328-64395347 None:intergenic 65.0%
GGTTGCAGGCGGTGGAATGG+TGG - chr1_1:64395331-64395350 None:intergenic 65.0%
GGTGGTTGCAGGCGGTGGAA+TGG - chr1_1:64395334-64395353 None:intergenic 65.0%
TTGGAGGTGGTTGCAGGCGG+TGG - chr1_1:64395339-64395358 None:intergenic 65.0%
GGGATGCTCGTCCTGCTAGG+TGG + chr1_1:64395492-64395511 Msa0036060:CDS 65.0%
GTCCCTCGGAACTCTCGTCC+GGG - chr1_1:64395628-64395647 None:intergenic 65.0%
CGTCCCTCGGAACTCTCGTC+CGG - chr1_1:64395629-64395648 None:intergenic 65.0%
GCGCCACTCGCTCTCGTCCT+TGG + chr1_1:64395225-64395244 Msa0036060:CDS 70.0%
!! AGGCGGTGGAATGGTGGCGG+CGG - chr1_1:64395325-64395344 None:intergenic 70.0%
CGGAATCCATCGCCGCCGCA+AGG - chr1_1:64395407-64395426 None:intergenic 70.0%
CGGCGGGCGGGGAAAGTGAG+AGG - chr1_1:64395308-64395327 None:intergenic 75.0%
!! TGGAATGGTGGCGGCGGGCG+GGG - chr1_1:64395319-64395338 None:intergenic 75.0%
!! GTGGAATGGTGGCGGCGGGC+GGG - chr1_1:64395320-64395339 None:intergenic 75.0%
!! GGTGGAATGGTGGCGGCGGG+CGG - chr1_1:64395321-64395340 None:intergenic 75.0%
!! GGCGGTGGAATGGTGGCGGC+GGG - chr1_1:64395324-64395343 None:intergenic 75.0%
CTGCGCTTGCCTTGCGCCGC+CGG + chr1_1:64395553-64395572 Msa0036060:CDS 75.0%
! TGGAGTCGGCGGCGCCGCCT+CGG - chr1_1:64395597-64395616 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 64394964 64397865 64394964 ID=Msa0036060;Name=Msa0036060
chr1_1 mRNA 64394964 64397865 64394964 ID=Msa0036060-mRNA-1;Parent=Msa0036060;Name=Msa0036060-mRNA-1;_AED=0.04;_eAED=0.04;_QI=245|1|1|1|0.75|0.6|5|504|305
chr1_1 exon 64394964 64395713 64394964 ID=Msa0036060-mRNA-1:exon:20615;Parent=Msa0036060-mRNA-1
chr1_1 exon 64396322 64396443 64396322 ID=Msa0036060-mRNA-1:exon:20616;Parent=Msa0036060-mRNA-1
chr1_1 exon 64396558 64396598 64396558 ID=Msa0036060-mRNA-1:exon:20617;Parent=Msa0036060-mRNA-1
chr1_1 exon 64396699 64396797 64396699 ID=Msa0036060-mRNA-1:exon:20618;Parent=Msa0036060-mRNA-1
chr1_1 exon 64397211 64397865 64397211 ID=Msa0036060-mRNA-1:exon:20619;Parent=Msa0036060-mRNA-1
chr1_1 five_prime_UTR 64394964 64395208 64394964 ID=Msa0036060-mRNA-1:five_prime_utr;Parent=Msa0036060-mRNA-1
chr1_1 CDS 64395209 64395713 64395209 ID=Msa0036060-mRNA-1:cds;Parent=Msa0036060-mRNA-1
chr1_1 CDS 64396322 64396443 64396322 ID=Msa0036060-mRNA-1:cds;Parent=Msa0036060-mRNA-1
chr1_1 CDS 64396558 64396598 64396558 ID=Msa0036060-mRNA-1:cds;Parent=Msa0036060-mRNA-1
chr1_1 CDS 64396699 64396797 64396699 ID=Msa0036060-mRNA-1:cds;Parent=Msa0036060-mRNA-1
chr1_1 CDS 64397211 64397361 64397211 ID=Msa0036060-mRNA-1:cds;Parent=Msa0036060-mRNA-1
chr1_1 three_prime_UTR 64397362 64397865 64397362 ID=Msa0036060-mRNA-1:three_prime_utr;Parent=Msa0036060-mRNA-1
Gene Sequence

>Msa0036060

ATGCTTGGTGTACTCTGCGCCACTCGCTCTCGTCCTTGGATCTTCTCTTCCCACCACCACCACCACGCCTTTCGTCTCTCTCACGCCACTGTCGCTCCTCTCACTTTCCCCGCCCGCCGCCACCATTCCACCGCCTGCAACCACCTCCAAATCTCAACCGGCGGTGGAGCTGCTTCGATATGGCACGCGATTACTCCTTGCGGCGGCGATGGATTCCGAAGCAGCGGTGTGATGCTTCATCATGACCACGAGCTCAAAGGTGAAGGATCGTGGAACGTTGCTTGGGATGCTCGTCCTGCTAGGTGGCTTCATCGTTCTGACTCAGCTTGGCTTCTTTTCGGTGTCTGCGCTTGCCTTGCGCCGCCGGTTATTGTTGACGTTGATACCGAGGCGGCGCCGCCGACTCCAGCTGTTTTCCCGGACGAGAGTTCCGAGGGACGTGAAGTGAAAGATGAGCTTTCCGATGAACGTGACGATGAACTCAACGCTGATTACAGAGTCACGGGGGTGCTTGCTGATGGTCGGTGTCTGTTCAGAGCAATAGCTCATGGAGCTTGCTTGAAAAATGGAGAGGAAGCGCCTAATGAAAGTCGACAGAGGGAACTTGCCGACGAATTAAGGGTTAAAGTTGCTGAAGAACTTTTGAACAGGCGCAAGGAAACAGAATGGTTTATAGAAGGGGATTTTGACACATATGTGACCAGAATTCAACAGACTTATGTTTGGGGAGGAGAGCCTGAGCTATTAATGGCTTCTCATGTTTTGAAGACTCCAATATATGTCTTCATGAGAGATGCAAGCTCCATGGATTTGGTAAATATAGCAAAATACGGTGAAGAATATATGAATGATGAGGAGATTTCCATCAATGTGCTATTTCATCGACATGGTCACTACGAACTATTGGAGACCTTATGA
Protein sequence

>Msa0036060

MLGVLCATRSRPWIFSSHHHHHAFRLSHATVAPLTFPARRHHSTACNHLQISTGGGAASIWHAITPCGGDGFRSSGVMLHHDHELKGEGSWNVAWDARPARWLHRSDSAWLLFGVCACLAPPVIVDVDTEAAPPTPAVFPDESSEGREVKDELSDERDDELNADYRVTGVLADGRCLFRAIAHGACLKNGEEAPNESRQRELADELRVKVAEELLNRRKETEWFIEGDFDTYVTRIQQTYVWGGEPELLMASHVLKTPIYVFMRDASSMDLVNIAKYGEEYMNDEEISINVLFHRHGHYELLETL*