AlfalfaGEDB | Msa0087360

M. sativa cultivar zhongmu-4 / Msa0087360

KEGG: -
GO: GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0012505 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791
PFAM: PF10250.12

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087360	sp\|Q8W486\|OFUT1_ARATH	77.626	514	108	3	1	508	1	513	0.0	816

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087360	A0A396K1R1	98.431	510	8	0	1	510	1	510	0.0	1051

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0039770	Msa0087360	0.933729	1.203771e-95	-8.615850e-47
Msa0087360	Msa0134110	0.881343	2.597774e-70	-8.615850e-47
Msa0087360	Msa0180990	0.897430	1.417212e-76	-8.615850e-47
Msa0087360	Msa1308330	0.804075	2.570691e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087360	MtrunA17_Chr1g0204121	98.431	510	8	0	1	510	1	510	0.0	1051
Msa0087360	MtrunA17_Chr1g0204141	91.634	514	38	2	1	510	1	513	0.0	980
Msa0087360	MtrunA17_Chr4g0042711	39.355	465	256	12	29	477	38	492	6.00e-98	305
Msa0087360	MtrunA17_Chr5g0417871	37.161	479	264	12	29	485	19	482	4.61e-91	286
Msa0087360	MtrunA17_Chr5g0426591	36.281	441	244	9	61	476	85	513	4.01e-89	283
Msa0087360	MtrunA17_Chr3g0143821	39.302	430	246	10	61	484	82	502	4.06e-89	284
Msa0087360	MtrunA17_Chr3g0129981	39.140	442	233	10	61	479	88	516	1.51e-88	281
Msa0087360	MtrunA17_Chr4g0070551	39.310	435	229	11	61	476	201	619	1.91e-88	283
Msa0087360	MtrunA17_Chr4g0056061	41.176	391	219	6	88	475	108	490	1.08e-86	276
Msa0087360	MtrunA17_Chr2g0307971	38.835	412	237	7	73	473	100	507	1.20e-85	273

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0087360	AT1G04910.1	77.626	514	108	3	1	508	1	513	0.0	816
Msa0087360	AT1G14020.1	39.750	400	223	8	88	475	91	484	1.05e-88	281
Msa0087360	AT5G35570.1	39.955	443	229	14	55	477	218	643	1.62e-87	282
Msa0087360	AT5G64600.1	37.838	444	230	12	61	476	91	516	5.92e-87	277
Msa0087360	AT4G24530.1	36.784	454	241	11	61	477	75	519	6.14e-86	275
Msa0087360	AT2G37980.1	37.808	447	245	11	49	477	187	618	6.76e-86	278
Msa0087360	AT2G03280.1	40.100	399	220	9	88	473	88	480	7.26e-86	273
Msa0087360	AT5G15740.1	40.000	405	223	9	84	473	99	498	8.77e-86	274
Msa0087360	AT4G38390.1	39.747	395	228	7	86	477	121	508	2.42e-85	274
Msa0087360	AT5G65470.1	35.575	461	251	12	54	476	52	504	4.32e-84	269

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Find 160 sgRNAs with CRISPR-Local

Find 419 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCTACACAAGCTTGAG+TGG	0.562467	1_2:+68295243	Msa0087360:exon
AAAGACCGAACTCCTATTAC+AGG	0.371904	1_2:+68295344	Msa0087360:three_prime_UTR
AAAGTTGCTTGGGCCATCAT+AGG	0.426197	1_2:-68294906	None:intergenic
AAATAGCCATTAGTCTTACT+CGG	0.584605	1_2:-68289740	None:intergenic
AAATATTCTGCATGATGAAT+TGG	0.310357	1_2:+68295201	Msa0087360:CDS
AACTGTGATTCTCAAGTCTA+GGG	0.546837	1_2:-68294784	None:intergenic
AACTTATAGGAGCATCTCTA+GGG	0.453270	1_2:-68292675	None:intergenic
AACTTTGCAAACAACCTCCT+TGG	0.484378	1_2:+68294923	Msa0087360:CDS
AAGAGCTTGCTGAGAACACT+AGG	0.471435	1_2:+68294822	Msa0087360:CDS
AAGCTTGAGTGGATATATAG+GGG	0.649342	1_2:+68295254	Msa0087360:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAAATTTACCTTCTCATGG+TGG	-	chr1_2:68289209-68289228	None:intergenic	30.0%
ACTACAAGAACAGATAGATA+CGG	-	chr1_2:68289626-68289645	None:intergenic	30.0%
TACGATATTGGATACTTGAT+GGG	-	chr1_2:68290036-68290055	None:intergenic	30.0%
AGTATCCAATATCGTAGTAT+TGG	+	chr1_2:68290040-68290059	Msa0087360:intron	30.0%
ATCGTATTTCGACTTAGATA+TGG	-	chr1_2:68290215-68290234	None:intergenic	30.0%
AATATATACCGATATAGGTG+GGG	+	chr1_2:68290308-68290327	Msa0087360:intron	30.0%
GCTGTAGATTTATATATGTG+TGG	+	chr1_2:68290363-68290382	Msa0087360:intron	30.0%
GTAGATTTATATATGTGTGG+TGG	+	chr1_2:68290366-68290385	Msa0087360:intron	30.0%
GTAGTGATCACATTCAAATT+GGG	-	chr1_2:68290673-68290692	None:intergenic	30.0%
AGTAGTGATCACATTCAAAT+TGG	-	chr1_2:68290674-68290693	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	68289039	68295768	68289039	ID=Msa0087360;Name=Msa0087360
chr1_2	mRNA	68289039	68295768	68289039	ID=Msa0087360-mRNA-1;Parent=Msa0087360;Name=Msa0087360-mRNA-1;_AED=0.06;_eAED=0.06;_QI=172\|1\|1\|1\|1\|1\|9\|507\|510
chr1_2	exon	68289039	68289218	68289039	ID=Msa0087360-mRNA-1:exon:23269;Parent=Msa0087360-mRNA-1
chr1_2	exon	68289299	68289458	68289299	ID=Msa0087360-mRNA-1:exon:23270;Parent=Msa0087360-mRNA-1
chr1_2	exon	68289546	68289624	68289546	ID=Msa0087360-mRNA-1:exon:23271;Parent=Msa0087360-mRNA-1
chr1_2	exon	68289735	68289805	68289735	ID=Msa0087360-mRNA-1:exon:23272;Parent=Msa0087360-mRNA-1
chr1_2	exon	68290439	68290527	68290439	ID=Msa0087360-mRNA-1:exon:23273;Parent=Msa0087360-mRNA-1
chr1_2	exon	68292452	68292575	68292452	ID=Msa0087360-mRNA-1:exon:23274;Parent=Msa0087360-mRNA-1
chr1_2	exon	68292667	68292950	68292667	ID=Msa0087360-mRNA-1:exon:23275;Parent=Msa0087360-mRNA-1
chr1_2	exon	68294366	68294495	68294366	ID=Msa0087360-mRNA-1:exon:23276;Parent=Msa0087360-mRNA-1
chr1_2	exon	68294674	68295768	68294674	ID=Msa0087360-mRNA-1:exon:23277;Parent=Msa0087360-mRNA-1
chr1_2	five_prime_UTR	68289039	68289210	68289039	ID=Msa0087360-mRNA-1:five_prime_utr;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68289211	68289218	68289211	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68289299	68289458	68289299	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68289546	68289624	68289546	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68289735	68289805	68289735	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68290439	68290527	68290439	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68292452	68292575	68292452	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68292667	68292950	68292667	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68294366	68294495	68294366	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	CDS	68294674	68295261	68294674	ID=Msa0087360-mRNA-1:cds;Parent=Msa0087360-mRNA-1
chr1_2	three_prime_UTR	68295262	68295768	68295262	ID=Msa0087360-mRNA-1:three_prime_utr;Parent=Msa0087360-mRNA-1

Gene Sequence

>Msa0087360

ATGAGAAGACCTGGACTTCACCGTCAGAATGGAAGACACATCGGAGGATCAGGATTCAAAGGAATGATTGCGAAGTTATCAATTGCTGCTATCGTACTCTTGATCTGCACGTTCTCATTGTTCTACTCTTCATCAAATGAGAATGTTCAATCCATTTTTCGATCAGAGATCCGTTTAGAAGAGCTTTGGAGCAATGCTGATTCCGGTGGTTGGCGACCGTCATCGTCGCCGCGTTCTCATTGGCCCCCTCCTCCGAGTAAGACTAATGGCTATTTGCGTGTTCGATGTAACGGTGGACTGAATCAGCAGCGTACTGCAATCTCTAATGCTGTTCTTGCTGCGCGGATCATGAATGCTACACTAGTGCTGCCTGAATTGGATGCAAACTCCTTCTGGCATGATGACAGTGGTTTCCATGGTATCTATGATGTTGAGCACTTCATTAGAACACTGAGGTTTGACGTAAAAATTGTAGAAAGCATTCCAGAAAATGAGAAAAATGGCAAAAAGAAGAAATTAAAGGCATTTCAGATCCGCCCCCCTAGAGATGCTCCTATAAGTTGGTATACTACTGATGCTCTCAAGAAAATGAAGGAACATGGTGCCATTTATCTTACTCCCTTTTCACACCGGTTAGCTGAAGAAATTGACAATCCTGAGTACCAAAGGTTGAGGTGTAGAGTTAACTATCATGCTTTAAGATTCAAGCCACATATTATGAAGCTAAGTCAATCAATAGTTGATAAGCTACGTGCACAAGGACCCTTCATGTCCATACATCTTCGGTTTGAGATGGATATGTTGTCATTTGCTGGATGCTTTGATATATTTACACCTGAGGAGCAAAAGATTCTGAAGAAATATCGAGAAGAAAATTTTGCACCTAAAAAACTAGTCTATAATGAAAGAAGAGCCATCGGCAAATGCCCATTGACTCCCGAGGAGGTTGGTCTTATTTTGCGTGCATTGGGTTTTGACAATTCTACCAGGATATATCTTGCAGCTGGAGAATTGTTTGGTGGGGATCGATTTATGAATCCATTTCGAAGTTTGTTCCCTAGACTTGAGAATCACAGTTCTGTAGATCACTCTGAAGAGCTTGCTGAGAACACTAGGGGGTTGGCAGGTTCTGCTGTCGATTACATGGTCTGCCTTCTTTCTGACATTTTCATGCCAACCTATGATGGCCCAAGCAACTTTGCAAACAACCTCCTTGGTCATCGCCTTTATTATGGTTTTCGGACTACAATCAGACCTGATAGAAAAGCTCTTGCCCCCATTTTCATTGATAGGGAGAATGGACGAATCCCCGATTTTGATGAGGCCGTTAGGAAAGTAATGCTTAAAACAAACTTTGGTGAACCTCATAAGCGTGTTTCTCCCGAGTCTTTCTATACAAATTCTTGGCCTGAATGCTTCTGTCAAACATCAGCAAAGAACCCAGAAGACAATTGTCCACCCAATGATGTTTTAAATATTCTGCATGATGAATTGGTAAAAGCATCTACAGACACAAACTCTACACAAGCTTGA

Protein sequence

>Msa0087360

MRRPGLHRQNGRHIGGSGFKGMIAKLSIAAIVLLICTFSLFYSSSNENVQSIFRSEIRLEELWSNADSGGWRPSSSPRSHWPPPPSKTNGYLRVRCNGGLNQQRTAISNAVLAARIMNATLVLPELDANSFWHDDSGFHGIYDVEHFIRTLRFDVKIVESIPENEKNGKKKKLKAFQIRPPRDAPISWYTTDALKKMKEHGAIYLTPFSHRLAEEIDNPEYQRLRCRVNYHALRFKPHIMKLSQSIVDKLRAQGPFMSIHLRFEMDMLSFAGCFDIFTPEEQKILKKYREENFAPKKLVYNERRAIGKCPLTPEEVGLILRALGFDNSTRIYLAAGELFGGDRFMNPFRSLFPRLENHSSVDHSEELAENTRGLAGSAVDYMVCLLSDIFMPTYDGPSNFANNLLGHRLYYGFRTTIRPDRKALAPIFIDRENGRIPDFDEAVRKVMLKTNFGEPHKRVSPESFYTNSWPECFCQTSAKNPEDNCPPNDVLNILHDELVKASTDTNSTQA*