Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0088790 | Msa0088800 | 0.890118 | 1.315355e-73 | -8.615850e-47 |
| Msa0088790 | Msa0135760 | 0.850559 | 1.570423e-60 | -8.615850e-47 |
| Msa0088790 | Msa0180380 | 0.878179 | 3.460827e-69 | -8.615850e-47 |
| Msa0088790 | Msa0637590 | 0.804610 | 1.988567e-49 | -8.615850e-47 |
| Msa0088790 | Msa0925940 | 0.804970 | 1.673092e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 5 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGTGTCTATTTGAGAATA+AGG | 0.239778 | 1_2:-69931517 | Msa0088790:CDS |
| ATAAGCACATCTGCTTCATT+AGG | 0.401944 | 1_2:-69931464 | Msa0088790:CDS |
| TAGACACTGATAAACATATA+CGG | 0.414211 | 1_2:+69931530 | None:intergenic |
| AGTACTTCAAGAAAAGTGTT+GGG | 0.536463 | 1_2:+69932764 | None:intergenic |
| CAGTACTTCAAGAAAAGTGT+TGG | 0.545004 | 1_2:+69932763 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAAATTAAAATCT+TGG | + | chr1_2:69931692-69931711 | None:intergenic | 10.0% |
| !!! | ATATATTTTGACATAAAAAA+TGG | - | chr1_2:69932189-69932208 | Msa0088790:intron | 10.0% |
| !!! | ATTTATTAATTTTGTGATTT+AGG | - | chr1_2:69932256-69932275 | Msa0088790:intron | 10.0% |
| !! | ACAAAATAAAATTAAAATCT+TGG | + | chr1_2:69931692-69931711 | None:intergenic | 10.0% |
| !!! | ATATATTTTGACATAAAAAA+TGG | - | chr1_2:69932189-69932208 | Msa0088790:intron | 10.0% |
| !!! | ATTTATTAATTTTGTGATTT+AGG | - | chr1_2:69932256-69932275 | Msa0088790:intron | 10.0% |
| !! | AATATCTAAGTTAATTTACT+AGG | - | chr1_2:69932460-69932479 | Msa0088790:intron | 15.0% |
| !! | GATATTTAATTAAATGACTA+AGG | + | chr1_2:69932522-69932541 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTTTTTCAGATAA+AGG | - | chr1_2:69932561-69932580 | Msa0088790:intron | 15.0% |
| !!! | AACATGAGTAATATTTTTTT+GGG | + | chr1_2:69932638-69932657 | None:intergenic | 15.0% |
| !! | AATATCTAAGTTAATTTACT+AGG | - | chr1_2:69932460-69932479 | Msa0088790:intron | 15.0% |
| !! | GATATTTAATTAAATGACTA+AGG | + | chr1_2:69932522-69932541 | None:intergenic | 15.0% |
| !!! | TTTTTTTCTTTTTCAGATAA+AGG | - | chr1_2:69932561-69932580 | Msa0088790:intron | 15.0% |
| !!! | AACATGAGTAATATTTTTTT+GGG | + | chr1_2:69932638-69932657 | None:intergenic | 15.0% |
| !! | TATATATTAAAGGAGAGAAA+TGG | - | chr1_2:69931885-69931904 | Msa0088790:intron | 20.0% |
| !! | AATATATATAATCAGTGCAT+TGG | - | chr1_2:69932044-69932063 | Msa0088790:intron | 20.0% |
| !!! | TATTTTGACATAAAAAATGG+AGG | - | chr1_2:69932192-69932211 | Msa0088790:intron | 20.0% |
| !!! | ATTTTGACATAAAAAATGGA+GGG | - | chr1_2:69932193-69932212 | Msa0088790:intron | 20.0% |
| !! | ATATCATAGCCTATTTATTA+AGG | + | chr1_2:69932347-69932366 | None:intergenic | 20.0% |
| !!! | TGTCATGTCTTATTTATTTT+CGG | - | chr1_2:69932393-69932412 | Msa0088790:intron | 20.0% |
| !!! | TTATTTATTTTCGGTTCAAT+TGG | - | chr1_2:69932402-69932421 | Msa0088790:intron | 20.0% |
| !!! | TATTTATTTTCGGTTCAATT+GGG | - | chr1_2:69932403-69932422 | Msa0088790:intron | 20.0% |
| !! | GAAAAAAAATCATCAAATCT+CGG | + | chr1_2:69932549-69932568 | None:intergenic | 20.0% |
| !!! | CAACATGAGTAATATTTTTT+TGG | + | chr1_2:69932639-69932658 | None:intergenic | 20.0% |
| !! | TATATATTAAAGGAGAGAAA+TGG | - | chr1_2:69931885-69931904 | Msa0088790:intron | 20.0% |
| !! | AATATATATAATCAGTGCAT+TGG | - | chr1_2:69932044-69932063 | Msa0088790:intron | 20.0% |
| !!! | TATTTTGACATAAAAAATGG+AGG | - | chr1_2:69932192-69932211 | Msa0088790:intron | 20.0% |
| !!! | ATTTTGACATAAAAAATGGA+GGG | - | chr1_2:69932193-69932212 | Msa0088790:intron | 20.0% |
| !! | ATATCATAGCCTATTTATTA+AGG | + | chr1_2:69932347-69932366 | None:intergenic | 20.0% |
| !!! | TGTCATGTCTTATTTATTTT+CGG | - | chr1_2:69932393-69932412 | Msa0088790:intron | 20.0% |
| !!! | TTATTTATTTTCGGTTCAAT+TGG | - | chr1_2:69932402-69932421 | Msa0088790:intron | 20.0% |
| !!! | TATTTATTTTCGGTTCAATT+GGG | - | chr1_2:69932403-69932422 | Msa0088790:intron | 20.0% |
| !! | GAAAAAAAATCATCAAATCT+CGG | + | chr1_2:69932549-69932568 | None:intergenic | 20.0% |
| !!! | CAACATGAGTAATATTTTTT+TGG | + | chr1_2:69932639-69932658 | None:intergenic | 20.0% |
| ! | TCAGTAATATCTCTATAAGA+GGG | + | chr1_2:69931623-69931642 | None:intergenic | 25.0% |
| ! | CATAAAAAGTTACCATTCAA+AGG | + | chr1_2:69932022-69932041 | None:intergenic | 25.0% |
| !!! | TGAATCCTTAAAGAGTATTT+CGG | - | chr1_2:69932110-69932129 | Msa0088790:intron | 25.0% |
| ! | TAAAGATCTCTTAAGAAAGA+CGG | - | chr1_2:69932221-69932240 | Msa0088790:intron | 25.0% |
| !!! | ATGAGTAATATTTTTTTGGG+TGG | + | chr1_2:69932635-69932654 | None:intergenic | 25.0% |
| ! | TAGACACTGATAAACATATA+CGG | + | chr1_2:69932679-69932698 | None:intergenic | 25.0% |
| ! | TCAGTAATATCTCTATAAGA+GGG | + | chr1_2:69931623-69931642 | None:intergenic | 25.0% |
| ! | CATAAAAAGTTACCATTCAA+AGG | + | chr1_2:69932022-69932041 | None:intergenic | 25.0% |
| !!! | TGAATCCTTAAAGAGTATTT+CGG | - | chr1_2:69932110-69932129 | Msa0088790:intron | 25.0% |
| ! | TAAAGATCTCTTAAGAAAGA+CGG | - | chr1_2:69932221-69932240 | Msa0088790:intron | 25.0% |
| !!! | ATGAGTAATATTTTTTTGGG+TGG | + | chr1_2:69932635-69932654 | None:intergenic | 25.0% |
| ! | TAGACACTGATAAACATATA+CGG | + | chr1_2:69932679-69932698 | None:intergenic | 25.0% |
| !! | AGTACTTCAAGAAAAGTGTT+GGG | + | chr1_2:69931445-69931464 | None:intergenic | 30.0% |
| !!! | CTTTTCTTGAAGTACTGTTA+TGG | - | chr1_2:69931449-69931468 | Msa0088790:CDS | 30.0% |
| AAATTACACACTCATCTCTT+AGG | - | chr1_2:69931523-69931542 | Msa0088790:CDS | 30.0% | |
| CAGTAATATCTCTATAAGAG+GGG | + | chr1_2:69931622-69931641 | None:intergenic | 30.0% | |
| CTCAGTAATATCTCTATAAG+AGG | + | chr1_2:69931624-69931643 | None:intergenic | 30.0% | |
| GTTGATGTGTCATATTTCAT+CGG | - | chr1_2:69931652-69931671 | Msa0088790:intron | 30.0% | |
| ! | GTTATTCTAGGGAAATGTTA+AGG | - | chr1_2:69931971-69931990 | Msa0088790:intron | 30.0% |
| CTCTTCCGTTTATTATTGTT+GGG | - | chr1_2:69932295-69932314 | Msa0088790:intron | 30.0% | |
| TCATGCCCAACAATAATAAA+CGG | + | chr1_2:69932303-69932322 | None:intergenic | 30.0% | |
| GCATGAATATGTGGATAATT+GGG | - | chr1_2:69932317-69932336 | Msa0088790:intron | 30.0% | |
| CATGAATATGTGGATAATTG+GGG | - | chr1_2:69932318-69932337 | Msa0088790:intron | 30.0% | |
| TCAGTGTCTATTTGAGAATA+AGG | - | chr1_2:69932689-69932708 | Msa0088790:intron | 30.0% | |
| !! | AGTACTTCAAGAAAAGTGTT+GGG | + | chr1_2:69931445-69931464 | None:intergenic | 30.0% |
| !!! | CTTTTCTTGAAGTACTGTTA+TGG | - | chr1_2:69931449-69931468 | Msa0088790:CDS | 30.0% |
| AAATTACACACTCATCTCTT+AGG | - | chr1_2:69931523-69931542 | Msa0088790:CDS | 30.0% | |
| CAGTAATATCTCTATAAGAG+GGG | + | chr1_2:69931622-69931641 | None:intergenic | 30.0% | |
| CTCAGTAATATCTCTATAAG+AGG | + | chr1_2:69931624-69931643 | None:intergenic | 30.0% | |
| GTTGATGTGTCATATTTCAT+CGG | - | chr1_2:69931652-69931671 | Msa0088790:intron | 30.0% | |
| ! | GTTATTCTAGGGAAATGTTA+AGG | - | chr1_2:69931971-69931990 | Msa0088790:intron | 30.0% |
| CTCTTCCGTTTATTATTGTT+GGG | - | chr1_2:69932295-69932314 | Msa0088790:intron | 30.0% | |
| TCATGCCCAACAATAATAAA+CGG | + | chr1_2:69932303-69932322 | None:intergenic | 30.0% | |
| GCATGAATATGTGGATAATT+GGG | - | chr1_2:69932317-69932336 | Msa0088790:intron | 30.0% | |
| CATGAATATGTGGATAATTG+GGG | - | chr1_2:69932318-69932337 | Msa0088790:intron | 30.0% | |
| TCAGTGTCTATTTGAGAATA+AGG | - | chr1_2:69932689-69932708 | Msa0088790:intron | 30.0% | |
| ! | CAGTACTTCAAGAAAAGTGT+TGG | + | chr1_2:69931446-69931465 | None:intergenic | 35.0% |
| ! | GAGGATAGTGTGTTATTCTA+GGG | - | chr1_2:69931960-69931979 | Msa0088790:intron | 35.0% |
| !! | AAATGTTAAGGAGTGTTTCC+AGG | - | chr1_2:69931983-69932002 | Msa0088790:intron | 35.0% |
| !! | AATGTTAAGGAGTGTTTCCA+GGG | - | chr1_2:69931984-69932003 | Msa0088790:intron | 35.0% |
| !! | CACTCTTTAAGACCTTTGAA+TGG | - | chr1_2:69932007-69932026 | Msa0088790:intron | 35.0% |
| TGTCTCCGAAATACTCTTTA+AGG | + | chr1_2:69932118-69932137 | None:intergenic | 35.0% | |
| CCAACAATAATAAACGGAAG+AGG | + | chr1_2:69932297-69932316 | None:intergenic | 35.0% | |
| CCTCTTCCGTTTATTATTGT+TGG | - | chr1_2:69932294-69932313 | Msa0088790:intron | 35.0% | |
| TATTGTTGGGCATGAATATG+TGG | - | chr1_2:69932308-69932327 | Msa0088790:intron | 35.0% | |
| GGCATGAATATGTGGATAAT+TGG | - | chr1_2:69932316-69932335 | Msa0088790:intron | 35.0% | |
| TTGGGGTGACCTTAATAAAT+AGG | - | chr1_2:69932335-69932354 | Msa0088790:intron | 35.0% | |
| ATAAGCACATCTGCTTCATT+AGG | - | chr1_2:69932742-69932761 | Msa0088790:intron | 35.0% | |
| ! | CAGTACTTCAAGAAAAGTGT+TGG | + | chr1_2:69931446-69931465 | None:intergenic | 35.0% |
| ! | GAGGATAGTGTGTTATTCTA+GGG | - | chr1_2:69931960-69931979 | Msa0088790:intron | 35.0% |
| !! | AAATGTTAAGGAGTGTTTCC+AGG | - | chr1_2:69931983-69932002 | Msa0088790:intron | 35.0% |
| !! | AATGTTAAGGAGTGTTTCCA+GGG | - | chr1_2:69931984-69932003 | Msa0088790:intron | 35.0% |
| !! | CACTCTTTAAGACCTTTGAA+TGG | - | chr1_2:69932007-69932026 | Msa0088790:intron | 35.0% |
| TGTCTCCGAAATACTCTTTA+AGG | + | chr1_2:69932118-69932137 | None:intergenic | 35.0% | |
| CCAACAATAATAAACGGAAG+AGG | + | chr1_2:69932297-69932316 | None:intergenic | 35.0% | |
| CCTCTTCCGTTTATTATTGT+TGG | - | chr1_2:69932294-69932313 | Msa0088790:intron | 35.0% | |
| TATTGTTGGGCATGAATATG+TGG | - | chr1_2:69932308-69932327 | Msa0088790:intron | 35.0% | |
| GGCATGAATATGTGGATAAT+TGG | - | chr1_2:69932316-69932335 | Msa0088790:intron | 35.0% | |
| TTGGGGTGACCTTAATAAAT+AGG | - | chr1_2:69932335-69932354 | Msa0088790:intron | 35.0% | |
| ATAAGCACATCTGCTTCATT+AGG | - | chr1_2:69932742-69932761 | Msa0088790:intron | 35.0% | |
| ! | GGAGGATAGTGTGTTATTCT+AGG | - | chr1_2:69931959-69931978 | Msa0088790:intron | 40.0% |
| ! | GGAGGATAGTGTGTTATTCT+AGG | - | chr1_2:69931959-69931978 | Msa0088790:intron | 40.0% |
| !! | AAAGGTCTTAAAGAGTGCCC+TGG | + | chr1_2:69932004-69932023 | None:intergenic | 45.0% |
| TTTCGGAGACACTCGTTAAC+AGG | - | chr1_2:69932127-69932146 | Msa0088790:intron | 45.0% | |
| !! | AAAGGTCTTAAAGAGTGCCC+TGG | + | chr1_2:69932004-69932023 | None:intergenic | 45.0% |
| TTTCGGAGACACTCGTTAAC+AGG | - | chr1_2:69932127-69932146 | Msa0088790:intron | 45.0% | |
| !! | AATAAAGAAATATATATTAA+AGG | - | chr1_2:69931875-69931894 | Msa0088790:intron | 5.0% |
| !! | AATTATAAAATTATAAAACA+TGG | + | chr1_2:69932497-69932516 | None:intergenic | 5.0% |
| !! | AATAAAGAAATATATATTAA+AGG | - | chr1_2:69931875-69931894 | Msa0088790:intron | 5.0% |
| !! | AATTATAAAATTATAAAACA+TGG | + | chr1_2:69932497-69932516 | None:intergenic | 5.0% |
| GTAATGCAAGCTCCTCTCTC+AGG | - | chr1_2:69931938-69931957 | Msa0088790:intron | 50.0% | |
| ACACACTATCCTCCTGAGAG+AGG | + | chr1_2:69931953-69931972 | None:intergenic | 50.0% | |
| GTAATGCAAGCTCCTCTCTC+AGG | - | chr1_2:69931938-69931957 | Msa0088790:intron | 50.0% | |
| ACACACTATCCTCCTGAGAG+AGG | + | chr1_2:69931953-69931972 | None:intergenic | 50.0% | |
| ATGCAAGCTCCTCTCTCAGG+AGG | - | chr1_2:69931941-69931960 | Msa0088790:intron | 55.0% | |
| ATGCAAGCTCCTCTCTCAGG+AGG | - | chr1_2:69931941-69931960 | Msa0088790:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_2 | gene | 69931436 | 69932792 | 69931436 | ID=Msa0088790;Name=Msa0088790 |
| chr1_2 | mRNA | 69931436 | 69932792 | 69931436 | ID=Msa0088790-mRNA-1;Parent=Msa0088790;Name=Msa0088790-mRNA-1;_AED=0.43;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|79 |
| chr1_2 | exon | 69932769 | 69932792 | 69932769 | ID=Msa0088790-mRNA-1:exon:24082;Parent=Msa0088790-mRNA-1 |
| chr1_2 | exon | 69931436 | 69931651 | 69931436 | ID=Msa0088790-mRNA-1:exon:24081;Parent=Msa0088790-mRNA-1 |
| chr1_2 | CDS | 69932769 | 69932792 | 69932769 | ID=Msa0088790-mRNA-1:cds;Parent=Msa0088790-mRNA-1 |
| chr1_2 | CDS | 69931436 | 69931651 | 69931436 | ID=Msa0088790-mRNA-1:cds;Parent=Msa0088790-mRNA-1 |
| Gene Sequence |
| Protein sequence |
