AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0089910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTGTTTCTTTGTTCTTT+TGG 0.118055 1_2:-71664902 None:intergenic
TTATACAGAGAATCAAAATT+AGG 0.181591 1_2:-71664927 None:intergenic
TTGTCAGAATTTATGAATTA+AGG 0.214442 1_2:+71665995 Msa0089910:three_prime_UTR
CACAGCAATGCCAAGGAAAT+TGG 0.216340 1_2:+71665750 Msa0089910:CDS
ATACTATTAATGTTTGAATT+AGG 0.219534 1_2:+71666081 Msa0089910:three_prime_UTR
CAAGGAAATTGGCTTCATTA+AGG 0.276234 1_2:+71665761 Msa0089910:CDS
TATACAGAGAATCAAAATTA+GGG 0.302066 1_2:-71664926 None:intergenic
AGCAAGGAAGTCATAGTAAA+AGG 0.306874 1_2:-71666046 None:intergenic
TTCTTTGCCCAGCCAATCTT+AGG 0.336932 1_2:+71665886 Msa0089910:CDS
GCCCAGCCAATCTTAGGTTT+CGG 0.337680 1_2:+71665892 Msa0089910:exon
CATGGATTGATAAGGAAGTA+TGG 0.378864 1_2:+71665702 Msa0089910:exon
CCATGGGACACTCAAATGTT+TGG 0.382308 1_2:+71665042 Msa0089910:five_prime_UTR
TAAGGCCATGGTATACAGAT+TGG 0.383167 1_2:+71666149 Msa0089910:three_prime_UTR
CTATACTGAGATTCTATTGT+TGG 0.405937 1_2:+71666358 Msa0089910:three_prime_UTR
GAACCAGGACCATAGGTCTT+GGG 0.410602 1_2:-71665074 None:intergenic
TGCCGAAACCTAAGATTGGC+TGG 0.421303 1_2:-71665894 None:intergenic
CTTAATGAAGCCAATTTCCT+TGG 0.421882 1_2:-71665760 None:intergenic
AAGGCCATGGTATACAGATT+GGG 0.453002 1_2:+71666150 Msa0089910:three_prime_UTR
AGCATTGCCTGCAGCACATA+AGG 0.453112 1_2:-71665725 None:intergenic
GGAACCAGGACCATAGGTCT+TGG 0.464100 1_2:-71665075 None:intergenic
ATGGTCCTGGTTCCCGCACT+TGG 0.475887 1_2:+71665084 Msa0089910:five_prime_UTR
AGACTCAAATGCTCTGCAGC+TGG 0.485140 1_2:-71664997 None:intergenic
CACCCCAAGACCTATGGTCC+TGG 0.491604 1_2:+71665071 Msa0089910:five_prime_UTR
TTCTTAAGTTTCTTCAACAT+AGG 0.492218 1_2:-71665951 None:intergenic
GCAGTCACAAACTACATTGT+CGG 0.501456 1_2:-71666324 None:intergenic
AACTCTCACCCCAAGACCTA+TGG 0.505813 1_2:+71665065 Msa0089910:five_prime_UTR
CTAATGCCGAAACCTAAGAT+TGG 0.515463 1_2:-71665898 None:intergenic
GGAATTCACATGGATTGATA+AGG 0.515737 1_2:+71665694 Msa0089910:five_prime_UTR
ATATACCAAGTGCGGGAACC+AGG 0.531737 1_2:-71665089 None:intergenic
AGTATGGCCTTATGTGCTGC+AGG 0.540472 1_2:+71665718 Msa0089910:exon
CCAAACATTTGAGTGTCCCA+TGG 0.543494 1_2:-71665042 None:intergenic
TGGTTTGTTGATTTGATCGC+CGG 0.550088 1_2:-71665022 None:intergenic
TGGCCTTAAAATTGCTGTGT+GGG 0.563277 1_2:-71666134 None:intergenic
TAACTTGTAGCCGTGTGTGC+GGG 0.573381 1_2:+71665673 Msa0089910:intron
GCGATCAAATCAACAAACCA+TGG 0.573789 1_2:+71665025 Msa0089910:five_prime_UTR
CATGTGAATTCCCGCACACA+CGG 0.574250 1_2:-71665683 None:intergenic
AAGAATGAATATACCAAGTG+CGG 0.579658 1_2:-71665097 None:intergenic
AGTGCGGGAACCAGGACCAT+AGG 0.585453 1_2:-71665081 None:intergenic
GCCGAAACCTAAGATTGGCT+GGG 0.586364 1_2:-71665893 None:intergenic
GCAGAGCATTTGAGTCTCAC+CGG 0.586976 1_2:+71665003 Msa0089910:five_prime_UTR
CAATTTCCTTGGCATTGCTG+TGG 0.593734 1_2:-71665749 None:intergenic
AACCAGGACCATAGGTCTTG+GGG 0.597207 1_2:-71665073 None:intergenic
CGTGTGTGCGGGAATTCACA+TGG 0.601988 1_2:+71665684 Msa0089910:five_prime_UTR
AGAATGAATATACCAAGTGC+GGG 0.603783 1_2:-71665096 None:intergenic
CAGCTGGAAAATTGTGTGGA+TGG 0.607918 1_2:-71664981 None:intergenic
CAAAGAAAATAGCTCTTCAA+CGG 0.615426 1_2:-71665870 None:intergenic
CGATCAAATCAACAAACCAT+GGG 0.616523 1_2:+71665026 Msa0089910:five_prime_UTR
ATGCTTCCACAGCAATGCCA+AGG 0.620494 1_2:+71665743 Msa0089910:CDS
GAAAATTGTGTGGATGGTGA+AGG 0.631842 1_2:-71664975 None:intergenic
ATAACTTGTAGCCGTGTGTG+CGG 0.665136 1_2:+71665672 Msa0089910:intron
CAAACCCAATCTGTATACCA+TGG 0.668308 1_2:-71666154 None:intergenic
TCTGCAGCTGGAAAATTGTG+TGG 0.680139 1_2:-71664985 None:intergenic
GGCCTTAAAATTGCTGTGTG+GGG 0.685788 1_2:-71666133 None:intergenic
ATGGCCTTAAAATTGCTGTG+TGG 0.697221 1_2:-71666135 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTATTGATAATATGTTTT+GGG + chr1_2:71665313-71665332 Msa0089910:intron 15.0%
!! AGGAAATGTATATATAAAAA+AGG - chr1_2:71666029-71666048 None:intergenic 15.0%
!! ATACTATTAATGTTTGAATT+AGG + chr1_2:71666081-71666100 Msa0089910:three_prime_UTR 15.0%
!!! TGTTATTGATAATATGTTTT+GGG + chr1_2:71665313-71665332 Msa0089910:intron 15.0%
!! AGGAAATGTATATATAAAAA+AGG - chr1_2:71666029-71666048 None:intergenic 15.0%
!! ATACTATTAATGTTTGAATT+AGG + chr1_2:71666081-71666100 Msa0089910:three_prime_UTR 15.0%
!! TATACAGAGAATCAAAATTA+GGG - chr1_2:71664929-71664948 None:intergenic 20.0%
!! TTATACAGAGAATCAAAATT+AGG - chr1_2:71664930-71664949 None:intergenic 20.0%
!!! TTTTGATTCTCTGTATAAAT+TGG + chr1_2:71664932-71664951 Msa0089910:five_prime_UTR 20.0%
!!! TAACCTTTTTCAATTCATTT+AGG + chr1_2:71665136-71665155 Msa0089910:intron 20.0%
!! AAACCTAAATGAATTGAAAA+AGG - chr1_2:71665142-71665161 None:intergenic 20.0%
!!! ATTTAGGTTTCTAGTATTTT+GGG + chr1_2:71665152-71665171 Msa0089910:intron 20.0%
!! GATTCAAAATTCATAAAAGA+GGG - chr1_2:71665212-71665231 None:intergenic 20.0%
!!! GTGTTATTGATAATATGTTT+TGG + chr1_2:71665312-71665331 Msa0089910:intron 20.0%
!!! GTTATTGATAATATGTTTTG+GGG + chr1_2:71665314-71665333 Msa0089910:intron 20.0%
!!! TTTTGGGGATTTTTATTTTT+TGG + chr1_2:71665329-71665348 Msa0089910:intron 20.0%
!!! TTTGGGGATTTTTATTTTTT+GGG + chr1_2:71665330-71665349 Msa0089910:intron 20.0%
!! TAATTAAGATTTGATTCAGA+TGG + chr1_2:71665394-71665413 Msa0089910:intron 20.0%
!!! TTGTGTTATTGCTATTATAT+CGG + chr1_2:71665499-71665518 Msa0089910:intron 20.0%
!! GCAACAAAAATAAAAACAAA+AGG - chr1_2:71665846-71665865 None:intergenic 20.0%
!! TTGTCAGAATTTATGAATTA+AGG + chr1_2:71665995-71666014 Msa0089910:three_prime_UTR 20.0%
!! TATACAGAGAATCAAAATTA+GGG - chr1_2:71664929-71664948 None:intergenic 20.0%
!! TTATACAGAGAATCAAAATT+AGG - chr1_2:71664930-71664949 None:intergenic 20.0%
!!! TTTTGATTCTCTGTATAAAT+TGG + chr1_2:71664932-71664951 Msa0089910:five_prime_UTR 20.0%
!!! TAACCTTTTTCAATTCATTT+AGG + chr1_2:71665136-71665155 Msa0089910:intron 20.0%
!! AAACCTAAATGAATTGAAAA+AGG - chr1_2:71665142-71665161 None:intergenic 20.0%
!!! ATTTAGGTTTCTAGTATTTT+GGG + chr1_2:71665152-71665171 Msa0089910:intron 20.0%
!! GATTCAAAATTCATAAAAGA+GGG - chr1_2:71665212-71665231 None:intergenic 20.0%
!!! GTGTTATTGATAATATGTTT+TGG + chr1_2:71665312-71665331 Msa0089910:intron 20.0%
!!! GTTATTGATAATATGTTTTG+GGG + chr1_2:71665314-71665333 Msa0089910:intron 20.0%
!!! TTTTGGGGATTTTTATTTTT+TGG + chr1_2:71665329-71665348 Msa0089910:intron 20.0%
!!! TTTGGGGATTTTTATTTTTT+GGG + chr1_2:71665330-71665349 Msa0089910:intron 20.0%
!! TAATTAAGATTTGATTCAGA+TGG + chr1_2:71665394-71665413 Msa0089910:intron 20.0%
!!! TTGTGTTATTGCTATTATAT+CGG + chr1_2:71665499-71665518 Msa0089910:intron 20.0%
!! GCAACAAAAATAAAAACAAA+AGG - chr1_2:71665846-71665865 None:intergenic 20.0%
!! TTGTCAGAATTTATGAATTA+AGG + chr1_2:71665995-71666014 Msa0089910:three_prime_UTR 20.0%
!!! TTCTGTTTCTTTGTTCTTTT+GGG - chr1_2:71664904-71664923 None:intergenic 25.0%
!!! TTTCTGTTTCTTTGTTCTTT+TGG - chr1_2:71664905-71664924 None:intergenic 25.0%
!!! CATTTAGGTTTCTAGTATTT+TGG + chr1_2:71665151-71665170 Msa0089910:intron 25.0%
! CAAAATGTAAACTTTAACAG+CGG - chr1_2:71665187-71665206 None:intergenic 25.0%
! CGATTCAAAATTCATAAAAG+AGG - chr1_2:71665213-71665232 None:intergenic 25.0%
! TTTGAACATATGTAGTTTAG+TGG + chr1_2:71665470-71665489 Msa0089910:intron 25.0%
! GATATAATAGCAATAACACA+AGG - chr1_2:71665500-71665519 None:intergenic 25.0%
!! ATTTTACATGATTGTATCTG+AGG - chr1_2:71665807-71665826 None:intergenic 25.0%
! TTCTTAAGTTTCTTCAACAT+AGG - chr1_2:71665954-71665973 None:intergenic 25.0%
! GTATATCACAAAAAATAGCA+AGG - chr1_2:71666065-71666084 None:intergenic 25.0%
!!! ATCAAAACATGCTGTTTTTT+CGG + chr1_2:71666274-71666293 Msa0089910:three_prime_UTR 25.0%
!!! TTCTGTTTCTTTGTTCTTTT+GGG - chr1_2:71664904-71664923 None:intergenic 25.0%
!!! TTTCTGTTTCTTTGTTCTTT+TGG - chr1_2:71664905-71664924 None:intergenic 25.0%
!!! CATTTAGGTTTCTAGTATTT+TGG + chr1_2:71665151-71665170 Msa0089910:intron 25.0%
! CAAAATGTAAACTTTAACAG+CGG - chr1_2:71665187-71665206 None:intergenic 25.0%
! CGATTCAAAATTCATAAAAG+AGG - chr1_2:71665213-71665232 None:intergenic 25.0%
! TTTGAACATATGTAGTTTAG+TGG + chr1_2:71665470-71665489 Msa0089910:intron 25.0%
! GATATAATAGCAATAACACA+AGG - chr1_2:71665500-71665519 None:intergenic 25.0%
!! ATTTTACATGATTGTATCTG+AGG - chr1_2:71665807-71665826 None:intergenic 25.0%
! TTCTTAAGTTTCTTCAACAT+AGG - chr1_2:71665954-71665973 None:intergenic 25.0%
! GTATATCACAAAAAATAGCA+AGG - chr1_2:71666065-71666084 None:intergenic 25.0%
!!! ATCAAAACATGCTGTTTTTT+CGG + chr1_2:71666274-71666293 Msa0089910:three_prime_UTR 25.0%
AAGAATGAATATACCAAGTG+CGG - chr1_2:71665100-71665119 None:intergenic 30.0%
!! GTGTTGTTTCTATGTTCTAA+TGG + chr1_2:71665241-71665260 Msa0089910:intron 30.0%
! CTAATGGTTTTCTATTGCTT+TGG + chr1_2:71665257-71665276 Msa0089910:intron 30.0%
CAAAGAAAATAGCTCTTCAA+CGG - chr1_2:71665873-71665892 None:intergenic 30.0%
AAGAATGAATATACCAAGTG+CGG - chr1_2:71665100-71665119 None:intergenic 30.0%
!! GTGTTGTTTCTATGTTCTAA+TGG + chr1_2:71665241-71665260 Msa0089910:intron 30.0%
! CTAATGGTTTTCTATTGCTT+TGG + chr1_2:71665257-71665276 Msa0089910:intron 30.0%
CAAAGAAAATAGCTCTTCAA+CGG - chr1_2:71665873-71665892 None:intergenic 30.0%
! TGTTTCTTTGTTCTTTTGGG+TGG - chr1_2:71664901-71664920 None:intergenic 35.0%
CGATCAAATCAACAAACCAT+GGG + chr1_2:71665026-71665045 Msa0089910:five_prime_UTR 35.0%
AGAATGAATATACCAAGTGC+GGG - chr1_2:71665099-71665118 None:intergenic 35.0%
!! TGCTTTGGTTTGTACTGTTA+TGG + chr1_2:71665272-71665291 Msa0089910:intron 35.0%
!! TTTTGGGTCAGCTTTGATAT+CGG + chr1_2:71665346-71665365 Msa0089910:intron 35.0%
TGCTATTATATCGGTACTAG+AGG + chr1_2:71665508-71665527 Msa0089910:intron 35.0%
! GGAATTCACATGGATTGATA+AGG + chr1_2:71665694-71665713 Msa0089910:five_prime_UTR 35.0%
! CATGGATTGATAAGGAAGTA+TGG + chr1_2:71665702-71665721 Msa0089910:exon 35.0%
CTTAATGAAGCCAATTTCCT+TGG - chr1_2:71665763-71665782 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG + chr1_2:71665761-71665780 Msa0089910:CDS 35.0%
AGCAAGGAAGTCATAGTAAA+AGG - chr1_2:71666049-71666068 None:intergenic 35.0%
! TGTTTCTTTGTTCTTTTGGG+TGG - chr1_2:71664901-71664920 None:intergenic 35.0%
CGATCAAATCAACAAACCAT+GGG + chr1_2:71665026-71665045 Msa0089910:five_prime_UTR 35.0%
AGAATGAATATACCAAGTGC+GGG - chr1_2:71665099-71665118 None:intergenic 35.0%
!! TGCTTTGGTTTGTACTGTTA+TGG + chr1_2:71665272-71665291 Msa0089910:intron 35.0%
!! TTTTGGGTCAGCTTTGATAT+CGG + chr1_2:71665346-71665365 Msa0089910:intron 35.0%
TGCTATTATATCGGTACTAG+AGG + chr1_2:71665508-71665527 Msa0089910:intron 35.0%
! GGAATTCACATGGATTGATA+AGG + chr1_2:71665694-71665713 Msa0089910:five_prime_UTR 35.0%
! CATGGATTGATAAGGAAGTA+TGG + chr1_2:71665702-71665721 Msa0089910:exon 35.0%
CTTAATGAAGCCAATTTCCT+TGG - chr1_2:71665763-71665782 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG + chr1_2:71665761-71665780 Msa0089910:CDS 35.0%
AGCAAGGAAGTCATAGTAAA+AGG - chr1_2:71666049-71666068 None:intergenic 35.0%
!! TTCTTTGTTCTTTTGGGTGG+TGG - chr1_2:71664898-71664917 None:intergenic 40.0%
GAAAATTGTGTGGATGGTGA+AGG - chr1_2:71664978-71664997 None:intergenic 40.0%
!! TGGTTTGTTGATTTGATCGC+CGG - chr1_2:71665025-71665044 None:intergenic 40.0%
GCGATCAAATCAACAAACCA+TGG + chr1_2:71665025-71665044 Msa0089910:five_prime_UTR 40.0%
CTAATGCCGAAACCTAAGAT+TGG - chr1_2:71665901-71665920 None:intergenic 40.0%
!! TTCGGCATTAGAGTGTTTTG+AGG + chr1_2:71665910-71665929 Msa0089910:three_prime_UTR 40.0%
! TGGCCTTAAAATTGCTGTGT+GGG - chr1_2:71666137-71666156 None:intergenic 40.0%
! ATGGCCTTAAAATTGCTGTG+TGG - chr1_2:71666138-71666157 None:intergenic 40.0%
!!! ACACAGCAATTTTAAGGCCA+TGG + chr1_2:71666137-71666156 Msa0089910:three_prime_UTR 40.0%
TAAGGCCATGGTATACAGAT+TGG + chr1_2:71666149-71666168 Msa0089910:three_prime_UTR 40.0%
AAGGCCATGGTATACAGATT+GGG + chr1_2:71666150-71666169 Msa0089910:three_prime_UTR 40.0%
CAAACCCAATCTGTATACCA+TGG - chr1_2:71666157-71666176 None:intergenic 40.0%
GCAGTCACAAACTACATTGT+CGG - chr1_2:71666327-71666346 None:intergenic 40.0%
!! TTCTTTGTTCTTTTGGGTGG+TGG - chr1_2:71664898-71664917 None:intergenic 40.0%
GAAAATTGTGTGGATGGTGA+AGG - chr1_2:71664978-71664997 None:intergenic 40.0%
!! TGGTTTGTTGATTTGATCGC+CGG - chr1_2:71665025-71665044 None:intergenic 40.0%
GCGATCAAATCAACAAACCA+TGG + chr1_2:71665025-71665044 Msa0089910:five_prime_UTR 40.0%
CTAATGCCGAAACCTAAGAT+TGG - chr1_2:71665901-71665920 None:intergenic 40.0%
!! TTCGGCATTAGAGTGTTTTG+AGG + chr1_2:71665910-71665929 Msa0089910:three_prime_UTR 40.0%
! TGGCCTTAAAATTGCTGTGT+GGG - chr1_2:71666137-71666156 None:intergenic 40.0%
! ATGGCCTTAAAATTGCTGTG+TGG - chr1_2:71666138-71666157 None:intergenic 40.0%
!!! ACACAGCAATTTTAAGGCCA+TGG + chr1_2:71666137-71666156 Msa0089910:three_prime_UTR 40.0%
TAAGGCCATGGTATACAGAT+TGG + chr1_2:71666149-71666168 Msa0089910:three_prime_UTR 40.0%
AAGGCCATGGTATACAGATT+GGG + chr1_2:71666150-71666169 Msa0089910:three_prime_UTR 40.0%
CAAACCCAATCTGTATACCA+TGG - chr1_2:71666157-71666176 None:intergenic 40.0%
GCAGTCACAAACTACATTGT+CGG - chr1_2:71666327-71666346 None:intergenic 40.0%
CAGCTGGAAAATTGTGTGGA+TGG - chr1_2:71664984-71665003 None:intergenic 45.0%
TCTGCAGCTGGAAAATTGTG+TGG - chr1_2:71664988-71665007 None:intergenic 45.0%
CCAAACATTTGAGTGTCCCA+TGG - chr1_2:71665045-71665064 None:intergenic 45.0%
CCATGGGACACTCAAATGTT+TGG + chr1_2:71665042-71665061 Msa0089910:five_prime_UTR 45.0%
! ATAACTTGTAGCCGTGTGTG+CGG + chr1_2:71665672-71665691 Msa0089910:intron 45.0%
CAATTTCCTTGGCATTGCTG+TGG - chr1_2:71665752-71665771 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG + chr1_2:71665750-71665769 Msa0089910:CDS 45.0%
TTCTTTGCCCAGCCAATCTT+AGG + chr1_2:71665886-71665905 Msa0089910:CDS 45.0%
!!! GGCATTAGAGTGTTTTGAGG+TGG + chr1_2:71665913-71665932 Msa0089910:three_prime_UTR 45.0%
! TGCCCCACACAGCAATTTTA+AGG + chr1_2:71666131-71666150 Msa0089910:three_prime_UTR 45.0%
GGCCTTAAAATTGCTGTGTG+GGG - chr1_2:71666136-71666155 None:intergenic 45.0%
CAGCTGGAAAATTGTGTGGA+TGG - chr1_2:71664984-71665003 None:intergenic 45.0%
TCTGCAGCTGGAAAATTGTG+TGG - chr1_2:71664988-71665007 None:intergenic 45.0%
CCAAACATTTGAGTGTCCCA+TGG - chr1_2:71665045-71665064 None:intergenic 45.0%
CCATGGGACACTCAAATGTT+TGG + chr1_2:71665042-71665061 Msa0089910:five_prime_UTR 45.0%
! ATAACTTGTAGCCGTGTGTG+CGG + chr1_2:71665672-71665691 Msa0089910:intron 45.0%
CAATTTCCTTGGCATTGCTG+TGG - chr1_2:71665752-71665771 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG + chr1_2:71665750-71665769 Msa0089910:CDS 45.0%
TTCTTTGCCCAGCCAATCTT+AGG + chr1_2:71665886-71665905 Msa0089910:CDS 45.0%
!!! GGCATTAGAGTGTTTTGAGG+TGG + chr1_2:71665913-71665932 Msa0089910:three_prime_UTR 45.0%
! TGCCCCACACAGCAATTTTA+AGG + chr1_2:71666131-71666150 Msa0089910:three_prime_UTR 45.0%
GGCCTTAAAATTGCTGTGTG+GGG - chr1_2:71666136-71666155 None:intergenic 45.0%
AGACTCAAATGCTCTGCAGC+TGG - chr1_2:71665000-71665019 None:intergenic 50.0%
! GCAGAGCATTTGAGTCTCAC+CGG + chr1_2:71665003-71665022 Msa0089910:five_prime_UTR 50.0%
AACTCTCACCCCAAGACCTA+TGG + chr1_2:71665065-71665084 Msa0089910:five_prime_UTR 50.0%
AACCAGGACCATAGGTCTTG+GGG - chr1_2:71665076-71665095 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG - chr1_2:71665077-71665096 None:intergenic 50.0%
ATATACCAAGTGCGGGAACC+AGG - chr1_2:71665092-71665111 None:intergenic 50.0%
! TAACTTGTAGCCGTGTGTGC+GGG + chr1_2:71665673-71665692 Msa0089910:intron 50.0%
CATGTGAATTCCCGCACACA+CGG - chr1_2:71665686-71665705 None:intergenic 50.0%
!! AGTATGGCCTTATGTGCTGC+AGG + chr1_2:71665718-71665737 Msa0089910:exon 50.0%
AGCATTGCCTGCAGCACATA+AGG - chr1_2:71665728-71665747 None:intergenic 50.0%
ATGCTTCCACAGCAATGCCA+AGG + chr1_2:71665743-71665762 Msa0089910:CDS 50.0%
GCCCAGCCAATCTTAGGTTT+CGG + chr1_2:71665892-71665911 Msa0089910:exon 50.0%
! GCCGAAACCTAAGATTGGCT+GGG - chr1_2:71665896-71665915 None:intergenic 50.0%
TGCCGAAACCTAAGATTGGC+TGG - chr1_2:71665897-71665916 None:intergenic 50.0%
AGACTCAAATGCTCTGCAGC+TGG - chr1_2:71665000-71665019 None:intergenic 50.0%
! GCAGAGCATTTGAGTCTCAC+CGG + chr1_2:71665003-71665022 Msa0089910:five_prime_UTR 50.0%
AACTCTCACCCCAAGACCTA+TGG + chr1_2:71665065-71665084 Msa0089910:five_prime_UTR 50.0%
AACCAGGACCATAGGTCTTG+GGG - chr1_2:71665076-71665095 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG - chr1_2:71665077-71665096 None:intergenic 50.0%
ATATACCAAGTGCGGGAACC+AGG - chr1_2:71665092-71665111 None:intergenic 50.0%
! TAACTTGTAGCCGTGTGTGC+GGG + chr1_2:71665673-71665692 Msa0089910:intron 50.0%
CATGTGAATTCCCGCACACA+CGG - chr1_2:71665686-71665705 None:intergenic 50.0%
!! AGTATGGCCTTATGTGCTGC+AGG + chr1_2:71665718-71665737 Msa0089910:exon 50.0%
AGCATTGCCTGCAGCACATA+AGG - chr1_2:71665728-71665747 None:intergenic 50.0%
ATGCTTCCACAGCAATGCCA+AGG + chr1_2:71665743-71665762 Msa0089910:CDS 50.0%
GCCCAGCCAATCTTAGGTTT+CGG + chr1_2:71665892-71665911 Msa0089910:exon 50.0%
! GCCGAAACCTAAGATTGGCT+GGG - chr1_2:71665896-71665915 None:intergenic 50.0%
TGCCGAAACCTAAGATTGGC+TGG - chr1_2:71665897-71665916 None:intergenic 50.0%
GGAACCAGGACCATAGGTCT+TGG - chr1_2:71665078-71665097 None:intergenic 55.0%
CGTGTGTGCGGGAATTCACA+TGG + chr1_2:71665684-71665703 Msa0089910:five_prime_UTR 55.0%
GGAACCAGGACCATAGGTCT+TGG - chr1_2:71665078-71665097 None:intergenic 55.0%
CGTGTGTGCGGGAATTCACA+TGG + chr1_2:71665684-71665703 Msa0089910:five_prime_UTR 55.0%
CACCCCAAGACCTATGGTCC+TGG + chr1_2:71665071-71665090 Msa0089910:five_prime_UTR 60.0%
AGTGCGGGAACCAGGACCAT+AGG - chr1_2:71665084-71665103 None:intergenic 60.0%
ATGGTCCTGGTTCCCGCACT+TGG + chr1_2:71665084-71665103 Msa0089910:five_prime_UTR 60.0%
CACCCCAAGACCTATGGTCC+TGG + chr1_2:71665071-71665090 Msa0089910:five_prime_UTR 60.0%
AGTGCGGGAACCAGGACCAT+AGG - chr1_2:71665084-71665103 None:intergenic 60.0%
ATGGTCCTGGTTCCCGCACT+TGG + chr1_2:71665084-71665103 Msa0089910:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 71664891 71666379 71664891 ID=Msa0089910;Name=Msa0089910
chr1_2 mRNA 71664891 71666379 71664891 ID=Msa0089910-mRNA-1;Parent=Msa0089910;Name=Msa0089910-mRNA-1;_AED=0.00;_eAED=0.00;_QI=253|1|1|1|0.5|0.66|3|472|33
chr1_2 exon 71664891 71665105 71664891 ID=Msa0089910-mRNA-1:exon:24680;Parent=Msa0089910-mRNA-1
chr1_2 exon 71665683 71665782 71665683 ID=Msa0089910-mRNA-1:exon:24681;Parent=Msa0089910-mRNA-1
chr1_2 exon 71665868 71666379 71665868 ID=Msa0089910-mRNA-1:exon:24682;Parent=Msa0089910-mRNA-1
chr1_2 five_prime_UTR 71664891 71665105 71664891 ID=Msa0089910-mRNA-1:five_prime_utr;Parent=Msa0089910-mRNA-1
chr1_2 five_prime_UTR 71665683 71665720 71665683 ID=Msa0089910-mRNA-1:five_prime_utr;Parent=Msa0089910-mRNA-1
chr1_2 CDS 71665721 71665782 71665721 ID=Msa0089910-mRNA-1:cds;Parent=Msa0089910-mRNA-1
chr1_2 CDS 71665868 71665907 71665868 ID=Msa0089910-mRNA-1:cds;Parent=Msa0089910-mRNA-1
chr1_2 three_prime_UTR 71665908 71666379 71665908 ID=Msa0089910-mRNA-1:three_prime_utr;Parent=Msa0089910-mRNA-1
Gene Sequence

>Msa0089910

ATGGCCTTATGTGCTGCAGGCAATGCTTCCACAGCAATGCCAAGGAAATTGGCTTCATTAAGTACCGTTGAAGAGCTATTTTCTTTGCCCAGCCAATCTTAG
Protein sequence

>Msa0089910

MALCAAGNASTAMPRKLASLSTVEELFSLPSQS*