AlfalfaGEDB | Msa0601130

M. sativa cultivar zhongmu-4 / Msa0601130

KEGG: K02955
GO: GO:0000028 GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006412 GO:0006518 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0015935 GO:0016043 GO:0016070 GO:0016072 GO:0019538 GO:0019843 GO:0022607 GO:0022613 GO:0022618 GO:0022626 GO:0022627 GO:0030490 GO:0031974 GO:0031981 GO:0032991 GO:0034470 GO:0034622 GO:0034641 GO:0034645 GO:0034660 GO:0042254 GO:0042255 GO:0042274 GO:0042788 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0046483 GO:0048027 GO:0065003 GO:0070013 GO:0070181 GO:0070925 GO:0071704 GO:0071826 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00411.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0601130	KAH0482163.1	90.698	43	4	0	10	52	22	64	1.52e-17	78.2

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0601130	sp\|Q54EG3\|RS14_DICDI	90.698	43	4	0	10	52	110	152	1.61e-20	80.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0089910	Msa0601130	0.848523	5.807407e-60	-8.615850e-47
Msa0136220	Msa0601130	0.803584	3.251425e-49	-8.615850e-47
Msa0162870	Msa0601130	0.821459	3.991907e-53	-8.615850e-47
Msa0373350	Msa0601130	0.814885	1.226616e-51	-8.615850e-47
Msa0585570	Msa0601130	0.810823	9.515319e-51	-8.615850e-47
Msa0601130	Msa0663400	0.802915	4.472184e-49	-8.615850e-47
Msa0601130	Msa1013430	0.841874	3.646579e-58	-8.615850e-47
Msa0601130	Msa1036880	0.801094	1.058885e-48	-8.615850e-47
Msa0601130	Msa1066350	0.832317	1.012730e-55	-8.615850e-47
Msa0601130	Msa1118790	0.805619	1.223023e-49	-8.615850e-47
Msa0601130	Msa1165210	0.809546	1.793634e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0601130	AT3G52580.1	93.750	32	2	10	41	108	139	5.14e-15	64.3
Msa0601130	AT3G11510.1	93.750	32	2	10	41	108	139	5.31e-15	64.3
Msa0601130	AT2G36160.1	93.750	32	2	10	41	108	139	5.79e-15	64.3

Find 39 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTAATTTGTAATGGTTTC+TGG	0.178922	4_2:+43584101	Msa0601130:three_prime_UTR
CCCTTATGAGTATAACTTAA+TGG	0.218904	4_2:+43583993	Msa0601130:three_prime_UTR
CTGAGTTTGCTATGGTATTA+TGG	0.243579	4_2:+43583710	Msa0601130:exon
ATAAACTCATGTAGAACTTT+TGG	0.286970	4_2:-43583858	None:intergenic
GGGTGCTGTCAGAAGGAATT+GGG	0.306077	4_2:-43583656	None:intergenic
TTTCTTTCCAGACTGTATTA+TGG	0.314408	4_2:+43583774	Msa0601130:three_prime_UTR
TACCCCTTCTACCACTCTTT+CGG	0.329294	4_2:-43583680	None:intergenic
CGGGTGCTGTCAGAAGGAAT+TGG	0.336102	4_2:-43583657	None:intergenic
CTGCAATCTACGAACCAATT+AGG	0.348949	4_2:-43583051	None:intergenic
GCCATTAAGTTATACTCATA+AGG	0.376078	4_2:-43583994	None:intergenic
CGGAGAGCAGCCTGAGCACC+AGG	0.397348	4_2:-43583334	None:intergenic
CTCCGAGCCTTGGCTCGGTC+AGG	0.414628	4_2:+43583352	Msa0601130:CDS
CCCGCCGAAAGAGTGGTAGA+AGG	0.418808	4_2:+43583676	Msa0601130:CDS
TCAGGCTGCTCTCCGAGCCT+TGG	0.439119	4_2:+43583342	Msa0601130:CDS
ACGTTGGTTGTAATTTGTAA+TGG	0.456750	4_2:+43584094	Msa0601130:three_prime_UTR
AGAGTGGTAGAAGGGGTAGA+AGG	0.460011	4_2:+43583685	Msa0601130:CDS
CCCTTCTACCACTCTTTCGG+CGG	0.475876	4_2:-43583677	None:intergenic
CCATTAAGTTATACTCATAA+GGG	0.478409	4_2:-43583993	None:intergenic
CTGCTCTCCGAGCCTTGGCT+CGG	0.481498	4_2:+43583347	Msa0601130:CDS
CAAATTACAACCAACGTAAG+TGG	0.493896	4_2:-43584059	None:intergenic
AGTGGGAATTTCACAGCCAT+TGG	0.498585	4_2:-43584041	None:intergenic
GAATATGCCATAATACAGTC+TGG	0.507182	4_2:-43583781	None:intergenic
CCTTCTACCACTCTTTCGGC+GGG	0.512822	4_2:-43583676	None:intergenic
GCTCGGTCAGGAATGAAAAT+CGG	0.525104	4_2:+43583364	Msa0601130:CDS
CCGCCGAAAGAGTGGTAGAA+GGG	0.532687	4_2:+43583677	Msa0601130:CDS
TTTCGGCGGGTGCTGTCAGA+AGG	0.542032	4_2:-43583663	None:intergenic
TTCCTGACCGAGCCAAGGCT+CGG	0.542845	4_2:-43583354	None:intergenic
GTTTGCTATGGTATTATGGA+AGG	0.571748	4_2:+43583714	Msa0601130:three_prime_UTR
AGAAGGCTCTGAGTTTGCTA+TGG	0.575425	4_2:+43583702	Msa0601130:exon
GACAGCACCCGCCGAAAGAG+TGG	0.584213	4_2:+43583669	Msa0601130:CDS
TTTCATTCCTGACCGAGCCA+AGG	0.585926	4_2:-43583359	None:intergenic
ACTTACTATTAAAATACCAA+TGG	0.590835	4_2:+43584025	Msa0601130:three_prime_UTR
AAATTACAACCAACGTAAGT+GGG	0.595719	4_2:-43584058	None:intergenic
GGAATGAAAATCGGTCGCAT+AGG	0.601542	4_2:+43583373	Msa0601130:CDS
GGTGCTGTCAGAAGGAATTG+GGG	0.625333	4_2:-43583655	None:intergenic
CGCCGAAAGAGTGGTAGAAG+GGG	0.627165	4_2:+43583678	Msa0601130:CDS
ATGTCTTGTAAAATGTCCAT+TGG	0.636230	4_2:-43583028	None:intergenic
GGAGAGCAGCCTGAGCACCA+GGG	0.647832	4_2:-43583333	None:intergenic
TGTGAAATTCCCACTTACGT+TGG	0.693028	4_2:+43584049	Msa0601130:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAAAATTAAATAAAAAAAA+TGG	+	chr4_2:43583119-43583138	Msa0601130:intron	0.0%
!!	ATAAAATTAAATAAAAAAAA+TGG	+	chr4_2:43583119-43583138	Msa0601130:intron	0.0%
!!	TTGTTTATATTTCATTCTAA+AGG	-	chr4_2:43583187-43583206	None:intergenic	15.0%
!!	TTGTTTATATTTCATTCTAA+AGG	-	chr4_2:43583187-43583206	None:intergenic	15.0%
!!!	NNNNNNGACTTTTCAACTAT+CGG	-	chr4_2:43583212-43583231	None:intergenic	20.0%
!!!	TATTCTTTTAGTCCATTTTT+AGG	+	chr4_2:43583396-43583415	Msa0601130:intron	20.0%
!!	AATACAAAATAAGAGTAACT+GGG	-	chr4_2:43583543-43583562	None:intergenic	20.0%
!!	CTGCAAATTTAAAAAACAAA+AGG	-	chr4_2:43583626-43583645	None:intergenic	20.0%
!!!	TTTGTTTTTTAAATTTGCAG+AGG	+	chr4_2:43583626-43583645	Msa0601130:intron	20.0%
!!!	TATTTGATTTACATCTTTTG+CGG	+	chr4_2:43583926-43583945	Msa0601130:three_prime_UTR	20.0%
!!	ACTTACTATTAAAATACCAA+TGG	+	chr4_2:43584025-43584044	Msa0601130:three_prime_UTR	20.0%
!!!	NNNNNNGACTTTTCAACTAT+CGG	-	chr4_2:43583212-43583231	None:intergenic	20.0%
!!!	TATTCTTTTAGTCCATTTTT+AGG	+	chr4_2:43583396-43583415	Msa0601130:intron	20.0%
!!	AATACAAAATAAGAGTAACT+GGG	-	chr4_2:43583543-43583562	None:intergenic	20.0%
!!	CTGCAAATTTAAAAAACAAA+AGG	-	chr4_2:43583626-43583645	None:intergenic	20.0%
!!!	TTTGTTTTTTAAATTTGCAG+AGG	+	chr4_2:43583626-43583645	Msa0601130:intron	20.0%
!!!	TATTTGATTTACATCTTTTG+CGG	+	chr4_2:43583926-43583945	Msa0601130:three_prime_UTR	20.0%
!!	ACTTACTATTAAAATACCAA+TGG	+	chr4_2:43584025-43584044	Msa0601130:three_prime_UTR	20.0%
!!	ATTTTACAAGACATCCTAAT+TGG	+	chr4_2:43583037-43583056	Msa0601130:CDS	25.0%
!	GAAAATAAGTTTAACCACTT+AGG	-	chr4_2:43583156-43583175	None:intergenic	25.0%
!	GAATACAAAATAAGAGTAAC+TGG	-	chr4_2:43583544-43583563	None:intergenic	25.0%
!!	ATAAACTCATGTAGAACTTT+TGG	-	chr4_2:43583861-43583880	None:intergenic	25.0%
!	CCATTAAGTTATACTCATAA+GGG	-	chr4_2:43583996-43584015	None:intergenic	25.0%
!!	TTGTAATTTGTAATGGTTTC+TGG	+	chr4_2:43584101-43584120	Msa0601130:three_prime_UTR	25.0%
!!	ATTTTACAAGACATCCTAAT+TGG	+	chr4_2:43583037-43583056	Msa0601130:CDS	25.0%
!	GAAAATAAGTTTAACCACTT+AGG	-	chr4_2:43583156-43583175	None:intergenic	25.0%
!	GAATACAAAATAAGAGTAAC+TGG	-	chr4_2:43583544-43583563	None:intergenic	25.0%
!!	ATAAACTCATGTAGAACTTT+TGG	-	chr4_2:43583861-43583880	None:intergenic	25.0%
!	CCATTAAGTTATACTCATAA+GGG	-	chr4_2:43583996-43584015	None:intergenic	25.0%
!!	TTGTAATTTGTAATGGTTTC+TGG	+	chr4_2:43584101-43584120	Msa0601130:three_prime_UTR	25.0%
	AAGCTAATTAGATACTAAGC+TGG	-	chr4_2:43583602-43583621	None:intergenic	30.0%
!!!	AGCTGCTTCTGTTTTTATTT+TGG	+	chr4_2:43583737-43583756	Msa0601130:three_prime_UTR	30.0%
	TCTGGAAAGAAACAAAAACA+CGG	-	chr4_2:43583766-43583785	None:intergenic	30.0%
	TTTCTTTCCAGACTGTATTA+TGG	+	chr4_2:43583774-43583793	Msa0601130:three_prime_UTR	30.0%
!	TGCTTCATCTAACTTTTGTT+CGG	+	chr4_2:43583960-43583979	Msa0601130:three_prime_UTR	30.0%
	CCCTTATGAGTATAACTTAA+TGG	+	chr4_2:43583993-43584012	Msa0601130:three_prime_UTR	30.0%
	GCCATTAAGTTATACTCATA+AGG	-	chr4_2:43583997-43584016	None:intergenic	30.0%
	AAATTACAACCAACGTAAGT+GGG	-	chr4_2:43584061-43584080	None:intergenic	30.0%
!!!	TTTGTTTTTTGAAGACACGT+TGG	+	chr4_2:43584078-43584097	Msa0601130:three_prime_UTR	30.0%
!	ACGTTGGTTGTAATTTGTAA+TGG	+	chr4_2:43584094-43584113	Msa0601130:three_prime_UTR	30.0%
	AAGCTAATTAGATACTAAGC+TGG	-	chr4_2:43583602-43583621	None:intergenic	30.0%
!!!	AGCTGCTTCTGTTTTTATTT+TGG	+	chr4_2:43583737-43583756	Msa0601130:three_prime_UTR	30.0%
	TCTGGAAAGAAACAAAAACA+CGG	-	chr4_2:43583766-43583785	None:intergenic	30.0%
	TTTCTTTCCAGACTGTATTA+TGG	+	chr4_2:43583774-43583793	Msa0601130:three_prime_UTR	30.0%
!	TGCTTCATCTAACTTTTGTT+CGG	+	chr4_2:43583960-43583979	Msa0601130:three_prime_UTR	30.0%
	CCCTTATGAGTATAACTTAA+TGG	+	chr4_2:43583993-43584012	Msa0601130:three_prime_UTR	30.0%
	GCCATTAAGTTATACTCATA+AGG	-	chr4_2:43583997-43584016	None:intergenic	30.0%
	AAATTACAACCAACGTAAGT+GGG	-	chr4_2:43584061-43584080	None:intergenic	30.0%
!!!	TTTGTTTTTTGAAGACACGT+TGG	+	chr4_2:43584078-43584097	Msa0601130:three_prime_UTR	30.0%
!	ACGTTGGTTGTAATTTGTAA+TGG	+	chr4_2:43584094-43584113	Msa0601130:three_prime_UTR	30.0%
	TGGTAAACAACTATCCTAAG+TGG	+	chr4_2:43583139-43583158	Msa0601130:intron	35.0%
	CAAAATAAGAGTAACTGGGA+AGG	-	chr4_2:43583539-43583558	None:intergenic	35.0%
!!	CTGAGTTTGCTATGGTATTA+TGG	+	chr4_2:43583710-43583729	Msa0601130:exon	35.0%
!!	GTTTGCTATGGTATTATGGA+AGG	+	chr4_2:43583714-43583733	Msa0601130:three_prime_UTR	35.0%
	GAATATGCCATAATACAGTC+TGG	-	chr4_2:43583784-43583803	None:intergenic	35.0%
	CAAATTACAACCAACGTAAG+TGG	-	chr4_2:43584062-43584081	None:intergenic	35.0%
	TGGTAAACAACTATCCTAAG+TGG	+	chr4_2:43583139-43583158	Msa0601130:intron	35.0%
	CAAAATAAGAGTAACTGGGA+AGG	-	chr4_2:43583539-43583558	None:intergenic	35.0%
!!	CTGAGTTTGCTATGGTATTA+TGG	+	chr4_2:43583710-43583729	Msa0601130:exon	35.0%
!!	GTTTGCTATGGTATTATGGA+AGG	+	chr4_2:43583714-43583733	Msa0601130:three_prime_UTR	35.0%
	GAATATGCCATAATACAGTC+TGG	-	chr4_2:43583784-43583803	None:intergenic	35.0%
	CAAATTACAACCAACGTAAG+TGG	-	chr4_2:43584062-43584081	None:intergenic	35.0%
	CTGCAATCTACGAACCAATT+AGG	-	chr4_2:43583054-43583073	None:intergenic	40.0%
	CACGACATGCATCCTAAAAA+TGG	-	chr4_2:43583411-43583430	None:intergenic	40.0%
!!!	CATTTTTAGGATGCATGTCG+TGG	+	chr4_2:43583409-43583428	Msa0601130:intron	40.0%
	TGTGAAATTCCCACTTACGT+TGG	+	chr4_2:43584049-43584068	Msa0601130:three_prime_UTR	40.0%
	CTGCAATCTACGAACCAATT+AGG	-	chr4_2:43583054-43583073	None:intergenic	40.0%
	CACGACATGCATCCTAAAAA+TGG	-	chr4_2:43583411-43583430	None:intergenic	40.0%
!!!	CATTTTTAGGATGCATGTCG+TGG	+	chr4_2:43583409-43583428	Msa0601130:intron	40.0%
	TGTGAAATTCCCACTTACGT+TGG	+	chr4_2:43584049-43584068	Msa0601130:three_prime_UTR	40.0%
	GCTCGGTCAGGAATGAAAAT+CGG	+	chr4_2:43583364-43583383	Msa0601130:CDS	45.0%
	GGAATGAAAATCGGTCGCAT+AGG	+	chr4_2:43583373-43583392	Msa0601130:CDS	45.0%
	TACCCCTTCTACCACTCTTT+CGG	-	chr4_2:43583683-43583702	None:intergenic	45.0%
!!	AGAAGGCTCTGAGTTTGCTA+TGG	+	chr4_2:43583702-43583721	Msa0601130:exon	45.0%
	AGTGGGAATTTCACAGCCAT+TGG	-	chr4_2:43584044-43584063	None:intergenic	45.0%
	GCTCGGTCAGGAATGAAAAT+CGG	+	chr4_2:43583364-43583383	Msa0601130:CDS	45.0%
	GGAATGAAAATCGGTCGCAT+AGG	+	chr4_2:43583373-43583392	Msa0601130:CDS	45.0%
	TACCCCTTCTACCACTCTTT+CGG	-	chr4_2:43583683-43583702	None:intergenic	45.0%
!!	AGAAGGCTCTGAGTTTGCTA+TGG	+	chr4_2:43583702-43583721	Msa0601130:exon	45.0%
	AGTGGGAATTTCACAGCCAT+TGG	-	chr4_2:43584044-43584063	None:intergenic	45.0%
	TTTCATTCCTGACCGAGCCA+AGG	-	chr4_2:43583362-43583381	None:intergenic	50.0%
	ATCACCAGCTTAGAGCAGCA+GGG	-	chr4_2:43583445-43583464	None:intergenic	50.0%
!	TCTAAGCTGGTGATGTGGTC+TGG	+	chr4_2:43583451-43583470	Msa0601130:intron	50.0%
!	GGTGCTGTCAGAAGGAATTG+GGG	-	chr4_2:43583658-43583677	None:intergenic	50.0%
!	GGGTGCTGTCAGAAGGAATT+GGG	-	chr4_2:43583659-43583678	None:intergenic	50.0%
	AGAGTGGTAGAAGGGGTAGA+AGG	+	chr4_2:43583685-43583704	Msa0601130:CDS	50.0%
	TTTCATTCCTGACCGAGCCA+AGG	-	chr4_2:43583362-43583381	None:intergenic	50.0%
	ATCACCAGCTTAGAGCAGCA+GGG	-	chr4_2:43583445-43583464	None:intergenic	50.0%
!	TCTAAGCTGGTGATGTGGTC+TGG	+	chr4_2:43583451-43583470	Msa0601130:intron	50.0%
!	GGTGCTGTCAGAAGGAATTG+GGG	-	chr4_2:43583658-43583677	None:intergenic	50.0%
!	GGGTGCTGTCAGAAGGAATT+GGG	-	chr4_2:43583659-43583678	None:intergenic	50.0%
	AGAGTGGTAGAAGGGGTAGA+AGG	+	chr4_2:43583685-43583704	Msa0601130:CDS	50.0%
!	TGTTCCCTGCTGCTCTAAGC+TGG	+	chr4_2:43583438-43583457	Msa0601130:intron	55.0%
	CATCACCAGCTTAGAGCAGC+AGG	-	chr4_2:43583446-43583465	None:intergenic	55.0%
!	GCTGCTCTAAGCTGGTGATG+TGG	+	chr4_2:43583446-43583465	Msa0601130:intron	55.0%
!	CGGGTGCTGTCAGAAGGAAT+TGG	-	chr4_2:43583660-43583679	None:intergenic	55.0%
	CCTTCTACCACTCTTTCGGC+GGG	-	chr4_2:43583679-43583698	None:intergenic	55.0%
	CCCTTCTACCACTCTTTCGG+CGG	-	chr4_2:43583680-43583699	None:intergenic	55.0%
	CCGCCGAAAGAGTGGTAGAA+GGG	+	chr4_2:43583677-43583696	Msa0601130:CDS	55.0%
	CGCCGAAAGAGTGGTAGAAG+GGG	+	chr4_2:43583678-43583697	Msa0601130:CDS	55.0%
!	TGTTCCCTGCTGCTCTAAGC+TGG	+	chr4_2:43583438-43583457	Msa0601130:intron	55.0%
	CATCACCAGCTTAGAGCAGC+AGG	-	chr4_2:43583446-43583465	None:intergenic	55.0%
!	GCTGCTCTAAGCTGGTGATG+TGG	+	chr4_2:43583446-43583465	Msa0601130:intron	55.0%
!	CGGGTGCTGTCAGAAGGAAT+TGG	-	chr4_2:43583660-43583679	None:intergenic	55.0%
	CCTTCTACCACTCTTTCGGC+GGG	-	chr4_2:43583679-43583698	None:intergenic	55.0%
	CCCTTCTACCACTCTTTCGG+CGG	-	chr4_2:43583680-43583699	None:intergenic	55.0%
	CCGCCGAAAGAGTGGTAGAA+GGG	+	chr4_2:43583677-43583696	Msa0601130:CDS	55.0%
	CGCCGAAAGAGTGGTAGAAG+GGG	+	chr4_2:43583678-43583697	Msa0601130:CDS	55.0%
	TTCCTGACCGAGCCAAGGCT+CGG	-	chr4_2:43583357-43583376	None:intergenic	60.0%
!	TTTCGGCGGGTGCTGTCAGA+AGG	-	chr4_2:43583666-43583685	None:intergenic	60.0%
	CCCGCCGAAAGAGTGGTAGA+AGG	+	chr4_2:43583676-43583695	Msa0601130:CDS	60.0%
	TTCCTGACCGAGCCAAGGCT+CGG	-	chr4_2:43583357-43583376	None:intergenic	60.0%
!	TTTCGGCGGGTGCTGTCAGA+AGG	-	chr4_2:43583666-43583685	None:intergenic	60.0%
	CCCGCCGAAAGAGTGGTAGA+AGG	+	chr4_2:43583676-43583695	Msa0601130:CDS	60.0%
	GGAGAGCAGCCTGAGCACCA+GGG	-	chr4_2:43583336-43583355	None:intergenic	65.0%
	TCAGGCTGCTCTCCGAGCCT+TGG	+	chr4_2:43583342-43583361	Msa0601130:CDS	65.0%
	CTGCTCTCCGAGCCTTGGCT+CGG	+	chr4_2:43583347-43583366	Msa0601130:CDS	65.0%
	GACAGCACCCGCCGAAAGAG+TGG	+	chr4_2:43583669-43583688	Msa0601130:CDS	65.0%
	GGAGAGCAGCCTGAGCACCA+GGG	-	chr4_2:43583336-43583355	None:intergenic	65.0%
	TCAGGCTGCTCTCCGAGCCT+TGG	+	chr4_2:43583342-43583361	Msa0601130:CDS	65.0%
	CTGCTCTCCGAGCCTTGGCT+CGG	+	chr4_2:43583347-43583366	Msa0601130:CDS	65.0%
	GACAGCACCCGCCGAAAGAG+TGG	+	chr4_2:43583669-43583688	Msa0601130:CDS	65.0%
	CGGAGAGCAGCCTGAGCACC+AGG	-	chr4_2:43583337-43583356	None:intergenic	70.0%
!!	CTCCGAGCCTTGGCTCGGTC+AGG	+	chr4_2:43583352-43583371	Msa0601130:CDS	70.0%
	CGGAGAGCAGCCTGAGCACC+AGG	-	chr4_2:43583337-43583356	None:intergenic	70.0%
!!	CTCCGAGCCTTGGCTCGGTC+AGG	+	chr4_2:43583352-43583371	Msa0601130:CDS	70.0%
	GCAGCCTGAGCACCAGGGCC+TGG	-	chr4_2:43583331-43583350	None:intergenic	75.0%
	GCAGCCTGAGCACCAGGGCC+TGG	-	chr4_2:43583331-43583350	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	43583031	43584133	43583031	ID=Msa0601130;Name=Msa0601130
chr4_2	mRNA	43583031	43584133	43583031	ID=Msa0601130-mRNA-1;Parent=Msa0601130;Name=Msa0601130-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0.5\|0.33\|0.66\|1\|1\|3\|420\|52
chr4_2	exon	43583031	43583058	43583031	ID=Msa0601130-mRNA-1:exon:9511;Parent=Msa0601130-mRNA-1
chr4_2	exon	43583332	43583394	43583332	ID=Msa0601130-mRNA-1:exon:9512;Parent=Msa0601130-mRNA-1
chr4_2	exon	43583646	43584133	43583646	ID=Msa0601130-mRNA-1:exon:9513;Parent=Msa0601130-mRNA-1
chr4_2	CDS	43583031	43583058	43583031	ID=Msa0601130-mRNA-1:cds;Parent=Msa0601130-mRNA-1
chr4_2	CDS	43583332	43583394	43583332	ID=Msa0601130-mRNA-1:cds;Parent=Msa0601130-mRNA-1
chr4_2	CDS	43583646	43583713	43583646	ID=Msa0601130-mRNA-1:cds;Parent=Msa0601130-mRNA-1
chr4_2	three_prime_UTR	43583714	43584133	43583714	ID=Msa0601130-mRNA-1:three_prime_utr;Parent=Msa0601130-mRNA-1

Gene Sequence	>Msa0601130 ATGGACATTTTACAAGACATCCTAATTGGCCCTGGTGCTCAGGCTGCTCTCCGAGCCTTGGCTCGGTCAGGAATGAAAATCGGTCGCATAGAGGATGTGACCCCAATTCCTTCTGACAGCACCCGCCGAAAGAGTGGTAGAAGGGGTAGAAGGCTCTGA
Protein sequence	>Msa0601130 MDILQDILIGPGAQAALRALARSGMKIGRIEDVTPIPSDSTRRKSGRRGRRL*