AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCTGTTTCTTTGTTCTTT+TGG 0.118055 1_3:+68054520 None:intergenic
TTATACAGAGAATCAAAATT+AGG 0.181591 1_3:+68054495 None:intergenic
TTGTCAGAATTTATGAATTA+AGG 0.214442 1_3:-68053426 Msa0136220:three_prime_UTR
CACAGCAATGCCAAGGAAAT+TGG 0.216340 1_3:-68053671 Msa0136220:CDS
ATACTATTAATGTTTGAATT+AGG 0.219534 1_3:-68053340 Msa0136220:three_prime_UTR
CAAGGAAATTGGCTTCATTA+AGG 0.276234 1_3:-68053660 Msa0136220:intron
TATACAGAGAATCAAAATTA+GGG 0.302066 1_3:+68054496 None:intergenic
AGCAAGGAAGTCATAGTAAA+AGG 0.306874 1_3:+68053375 None:intergenic
TTCTTTGCCCAGCCAATCTT+AGG 0.336932 1_3:-68053535 Msa0136220:exon
GCCCAGCCAATCTTAGGTTT+CGG 0.337680 1_3:-68053529 Msa0136220:exon
CATGGATTGATAAGGAAGTA+TGG 0.378864 1_3:-68053719 Msa0136220:exon
CCATGGGACACTCAAATGTT+TGG 0.382308 1_3:-68054380 Msa0136220:five_prime_UTR
TAAGGCCATGGTATACAGAT+TGG 0.383167 1_3:-68053272 Msa0136220:three_prime_UTR
CTATACTGAGATTCTATTGT+TGG 0.405937 1_3:-68053063 None:intergenic
GAACCAGGACCATAGGTCTT+GGG 0.410602 1_3:+68054348 None:intergenic
TGCCGAAACCTAAGATTGGC+TGG 0.421303 1_3:+68053527 None:intergenic
CTTAATGAAGCCAATTTCCT+TGG 0.421882 1_3:+68053661 None:intergenic
AAGGCCATGGTATACAGATT+GGG 0.453002 1_3:-68053271 Msa0136220:three_prime_UTR
AGCATTGCCTGCAGCACATA+AGG 0.453112 1_3:+68053696 None:intergenic
GGAACCAGGACCATAGGTCT+TGG 0.464100 1_3:+68054347 None:intergenic
ATGGTCCTGGTTCCCGCACT+TGG 0.475887 1_3:-68054338 Msa0136220:intron
AGACTCAAATGCTCTGCAGC+TGG 0.485140 1_3:+68054425 None:intergenic
CACCCCAAGACCTATGGTCC+TGG 0.491604 1_3:-68054351 Msa0136220:five_prime_UTR
TTCTTAAGTTTCTTCAACAT+AGG 0.492218 1_3:+68053470 None:intergenic
GCAGTCACAAACTACATTGT+CGG 0.501456 1_3:+68053097 None:intergenic
AACTCTCACCCCAAGACCTA+TGG 0.505813 1_3:-68054357 Msa0136220:five_prime_UTR
CTAATGCCGAAACCTAAGAT+TGG 0.515463 1_3:+68053523 None:intergenic
GGAATTCACATGGATTGATA+AGG 0.515737 1_3:-68053727 Msa0136220:five_prime_UTR
ATATACCAAGTGCGGGAACC+AGG 0.531737 1_3:+68054333 None:intergenic
AGTATGGCCTTATGTGCTGC+AGG 0.540472 1_3:-68053703 Msa0136220:CDS
CCAAACATTTGAGTGTCCCA+TGG 0.543494 1_3:+68054380 None:intergenic
TGGTTTGTTGATTTGATCGC+CGG 0.550088 1_3:+68054400 None:intergenic
TGGCCTTAAAATTGCTGTGT+GGG 0.563277 1_3:+68053287 None:intergenic
TAACTTGTAGCCGTGTGTGC+GGG 0.573381 1_3:-68053748 Msa0136220:intron
GCGATCAAATCAACAAACCA+TGG 0.573789 1_3:-68054397 Msa0136220:five_prime_UTR
CATGTGAATTCCCGCACACA+CGG 0.574250 1_3:+68053738 None:intergenic
AAGAATGAATATACCAAGTG+CGG 0.579658 1_3:+68054325 None:intergenic
AGTGCGGGAACCAGGACCAT+AGG 0.585453 1_3:+68054341 None:intergenic
GCCGAAACCTAAGATTGGCT+GGG 0.586364 1_3:+68053528 None:intergenic
GCAGAGCATTTGAGTCTCAC+CGG 0.586976 1_3:-68054419 Msa0136220:five_prime_UTR
CAATTTCCTTGGCATTGCTG+TGG 0.593734 1_3:+68053672 None:intergenic
AACCAGGACCATAGGTCTTG+GGG 0.597207 1_3:+68054349 None:intergenic
CGTGTGTGCGGGAATTCACA+TGG 0.601988 1_3:-68053737 Msa0136220:five_prime_UTR
AGAATGAATATACCAAGTGC+GGG 0.603783 1_3:+68054326 None:intergenic
CAGCTGGAAAATTGTGTGGA+TGG 0.607918 1_3:+68054441 None:intergenic
CAAAGAAAATAGCTCTTCAA+CGG 0.615426 1_3:+68053551 None:intergenic
CGATCAAATCAACAAACCAT+GGG 0.616523 1_3:-68054396 Msa0136220:five_prime_UTR
ATGCTTCCACAGCAATGCCA+AGG 0.620494 1_3:-68053678 Msa0136220:CDS
GAAAATTGTGTGGATGGTGA+AGG 0.631842 1_3:+68054447 None:intergenic
ATAACTTGTAGCCGTGTGTG+CGG 0.665136 1_3:-68053749 Msa0136220:intron
CAAACCCAATCTGTATACCA+TGG 0.668308 1_3:+68053267 None:intergenic
TCTGCAGCTGGAAAATTGTG+TGG 0.680139 1_3:+68054437 None:intergenic
GGCCTTAAAATTGCTGTGTG+GGG 0.685788 1_3:+68053288 None:intergenic
ATGGCCTTAAAATTGCTGTG+TGG 0.697221 1_3:+68053286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTATTGATAATATGTTTT+GGG - chr1_3:68053486-68053505 Msa0136220:three_prime_UTR 15.0%
!! AGGAAATGTATATATAAAAA+AGG + chr1_3:68054203-68054222 None:intergenic 15.0%
!! ATACTATTAATGTTTGAATT+AGG - chr1_3:68054255-68054274 Msa0136220:intron 15.0%
!!! TGTTATTGATAATATGTTTT+GGG - chr1_3:68053486-68053505 Msa0136220:three_prime_UTR 15.0%
!! AGGAAATGTATATATAAAAA+AGG + chr1_3:68054203-68054222 None:intergenic 15.0%
!! ATACTATTAATGTTTGAATT+AGG - chr1_3:68054255-68054274 Msa0136220:intron 15.0%
!! TATACAGAGAATCAAAATTA+GGG + chr1_3:68053102-68053121 None:intergenic 20.0%
!! TTATACAGAGAATCAAAATT+AGG + chr1_3:68053103-68053122 None:intergenic 20.0%
!!! TTTTGATTCTCTGTATAAAT+TGG - chr1_3:68053105-68053124 Msa0136220:three_prime_UTR 20.0%
!!! TAACCTTTTTCAATTCATTT+AGG - chr1_3:68053309-68053328 Msa0136220:three_prime_UTR 20.0%
!! AAACCTAAATGAATTGAAAA+AGG + chr1_3:68053315-68053334 None:intergenic 20.0%
!!! ATTTAGGTTTCTAGTATTTT+GGG - chr1_3:68053325-68053344 Msa0136220:three_prime_UTR 20.0%
!! GATTCAAAATTCATAAAAGA+GGG + chr1_3:68053385-68053404 None:intergenic 20.0%
!!! GTGTTATTGATAATATGTTT+TGG - chr1_3:68053485-68053504 Msa0136220:three_prime_UTR 20.0%
!!! GTTATTGATAATATGTTTTG+GGG - chr1_3:68053487-68053506 Msa0136220:three_prime_UTR 20.0%
!!! TTTGGGGATTTTTATTTTTT+TGG - chr1_3:68053503-68053522 Msa0136220:three_prime_UTR 20.0%
!!! TTGGGGATTTTTATTTTTTT+GGG - chr1_3:68053504-68053523 Msa0136220:three_prime_UTR 20.0%
!! TAATTAAGATTTGATTCAGA+TGG - chr1_3:68053568-68053587 Msa0136220:intron 20.0%
!!! TTGTGTTATTGCTATTATAT+CGG - chr1_3:68053673-68053692 Msa0136220:CDS 20.0%
!! GCAACAAAAATAAAAACAAA+AGG + chr1_3:68054020-68054039 None:intergenic 20.0%
!! TTGTCAGAATTTATGAATTA+AGG - chr1_3:68054169-68054188 Msa0136220:intron 20.0%
!! TATACAGAGAATCAAAATTA+GGG + chr1_3:68053102-68053121 None:intergenic 20.0%
!! TTATACAGAGAATCAAAATT+AGG + chr1_3:68053103-68053122 None:intergenic 20.0%
!!! TTTTGATTCTCTGTATAAAT+TGG - chr1_3:68053105-68053124 Msa0136220:three_prime_UTR 20.0%
!!! TAACCTTTTTCAATTCATTT+AGG - chr1_3:68053309-68053328 Msa0136220:three_prime_UTR 20.0%
!! AAACCTAAATGAATTGAAAA+AGG + chr1_3:68053315-68053334 None:intergenic 20.0%
!!! ATTTAGGTTTCTAGTATTTT+GGG - chr1_3:68053325-68053344 Msa0136220:three_prime_UTR 20.0%
!! GATTCAAAATTCATAAAAGA+GGG + chr1_3:68053385-68053404 None:intergenic 20.0%
!!! GTGTTATTGATAATATGTTT+TGG - chr1_3:68053485-68053504 Msa0136220:three_prime_UTR 20.0%
!!! GTTATTGATAATATGTTTTG+GGG - chr1_3:68053487-68053506 Msa0136220:three_prime_UTR 20.0%
!!! TTTGGGGATTTTTATTTTTT+TGG - chr1_3:68053503-68053522 Msa0136220:three_prime_UTR 20.0%
!!! TTGGGGATTTTTATTTTTTT+GGG - chr1_3:68053504-68053523 Msa0136220:three_prime_UTR 20.0%
!! TAATTAAGATTTGATTCAGA+TGG - chr1_3:68053568-68053587 Msa0136220:intron 20.0%
!!! TTGTGTTATTGCTATTATAT+CGG - chr1_3:68053673-68053692 Msa0136220:CDS 20.0%
!! GCAACAAAAATAAAAACAAA+AGG + chr1_3:68054020-68054039 None:intergenic 20.0%
!! TTGTCAGAATTTATGAATTA+AGG - chr1_3:68054169-68054188 Msa0136220:intron 20.0%
!!! TTCTGTTTCTTTGTTCTTTT+GGG + chr1_3:68053077-68053096 None:intergenic 25.0%
!!! TTTCTGTTTCTTTGTTCTTT+TGG + chr1_3:68053078-68053097 None:intergenic 25.0%
!!! CATTTAGGTTTCTAGTATTT+TGG - chr1_3:68053324-68053343 Msa0136220:three_prime_UTR 25.0%
! CAAAATGTAAACTTTAACAG+CGG + chr1_3:68053360-68053379 None:intergenic 25.0%
! CGATTCAAAATTCATAAAAG+AGG + chr1_3:68053386-68053405 None:intergenic 25.0%
! TTTGAACATATGTAGTTTAG+TGG - chr1_3:68053644-68053663 Msa0136220:intron 25.0%
! GATATAATAGCAATAACACA+AGG + chr1_3:68053674-68053693 None:intergenic 25.0%
!! ATTTTACATGATTGTATCTG+AGG + chr1_3:68053981-68054000 None:intergenic 25.0%
! TTCTTAAGTTTCTTCAACAT+AGG + chr1_3:68054128-68054147 None:intergenic 25.0%
! GTATATCACAAAAAATAGCA+AGG + chr1_3:68054239-68054258 None:intergenic 25.0%
!!! ATCAAAACATGCTGTTTTTT+CGG - chr1_3:68054448-68054467 Msa0136220:five_prime_UTR 25.0%
!!! TTCTGTTTCTTTGTTCTTTT+GGG + chr1_3:68053077-68053096 None:intergenic 25.0%
!!! TTTCTGTTTCTTTGTTCTTT+TGG + chr1_3:68053078-68053097 None:intergenic 25.0%
!!! CATTTAGGTTTCTAGTATTT+TGG - chr1_3:68053324-68053343 Msa0136220:three_prime_UTR 25.0%
! CAAAATGTAAACTTTAACAG+CGG + chr1_3:68053360-68053379 None:intergenic 25.0%
! CGATTCAAAATTCATAAAAG+AGG + chr1_3:68053386-68053405 None:intergenic 25.0%
! TTTGAACATATGTAGTTTAG+TGG - chr1_3:68053644-68053663 Msa0136220:intron 25.0%
! GATATAATAGCAATAACACA+AGG + chr1_3:68053674-68053693 None:intergenic 25.0%
!! ATTTTACATGATTGTATCTG+AGG + chr1_3:68053981-68054000 None:intergenic 25.0%
! TTCTTAAGTTTCTTCAACAT+AGG + chr1_3:68054128-68054147 None:intergenic 25.0%
! GTATATCACAAAAAATAGCA+AGG + chr1_3:68054239-68054258 None:intergenic 25.0%
!!! ATCAAAACATGCTGTTTTTT+CGG - chr1_3:68054448-68054467 Msa0136220:five_prime_UTR 25.0%
AAGAATGAATATACCAAGTG+CGG + chr1_3:68053273-68053292 None:intergenic 30.0%
!! GTGTTGTTTCTATGTTCTAA+TGG - chr1_3:68053414-68053433 Msa0136220:three_prime_UTR 30.0%
! CTAATGGTTTTCTATTGCTT+TGG - chr1_3:68053430-68053449 Msa0136220:three_prime_UTR 30.0%
CAAAGAAAATAGCTCTTCAA+CGG + chr1_3:68054047-68054066 None:intergenic 30.0%
AAGAATGAATATACCAAGTG+CGG + chr1_3:68053273-68053292 None:intergenic 30.0%
!! GTGTTGTTTCTATGTTCTAA+TGG - chr1_3:68053414-68053433 Msa0136220:three_prime_UTR 30.0%
! CTAATGGTTTTCTATTGCTT+TGG - chr1_3:68053430-68053449 Msa0136220:three_prime_UTR 30.0%
CAAAGAAAATAGCTCTTCAA+CGG + chr1_3:68054047-68054066 None:intergenic 30.0%
! TGTTTCTTTGTTCTTTTGGG+TGG + chr1_3:68053074-68053093 None:intergenic 35.0%
CGATCAAATCAACAAACCAT+GGG - chr1_3:68053199-68053218 Msa0136220:three_prime_UTR 35.0%
AGAATGAATATACCAAGTGC+GGG + chr1_3:68053272-68053291 None:intergenic 35.0%
!! TGCTTTGGTTTGTACTGTTA+TGG - chr1_3:68053445-68053464 Msa0136220:three_prime_UTR 35.0%
!! TTTTGGGTCAGCTTTGATAT+CGG - chr1_3:68053520-68053539 Msa0136220:exon 35.0%
TGCTATTATATCGGTACTAG+AGG - chr1_3:68053682-68053701 Msa0136220:CDS 35.0%
! GGAATTCACATGGATTGATA+AGG - chr1_3:68053868-68053887 Msa0136220:intron 35.0%
! CATGGATTGATAAGGAAGTA+TGG - chr1_3:68053876-68053895 Msa0136220:intron 35.0%
CTTAATGAAGCCAATTTCCT+TGG + chr1_3:68053937-68053956 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG - chr1_3:68053935-68053954 Msa0136220:intron 35.0%
AGCAAGGAAGTCATAGTAAA+AGG + chr1_3:68054223-68054242 None:intergenic 35.0%
! TGTTTCTTTGTTCTTTTGGG+TGG + chr1_3:68053074-68053093 None:intergenic 35.0%
CGATCAAATCAACAAACCAT+GGG - chr1_3:68053199-68053218 Msa0136220:three_prime_UTR 35.0%
AGAATGAATATACCAAGTGC+GGG + chr1_3:68053272-68053291 None:intergenic 35.0%
!! TGCTTTGGTTTGTACTGTTA+TGG - chr1_3:68053445-68053464 Msa0136220:three_prime_UTR 35.0%
!! TTTTGGGTCAGCTTTGATAT+CGG - chr1_3:68053520-68053539 Msa0136220:exon 35.0%
TGCTATTATATCGGTACTAG+AGG - chr1_3:68053682-68053701 Msa0136220:CDS 35.0%
! GGAATTCACATGGATTGATA+AGG - chr1_3:68053868-68053887 Msa0136220:intron 35.0%
! CATGGATTGATAAGGAAGTA+TGG - chr1_3:68053876-68053895 Msa0136220:intron 35.0%
CTTAATGAAGCCAATTTCCT+TGG + chr1_3:68053937-68053956 None:intergenic 35.0%
! CAAGGAAATTGGCTTCATTA+AGG - chr1_3:68053935-68053954 Msa0136220:intron 35.0%
AGCAAGGAAGTCATAGTAAA+AGG + chr1_3:68054223-68054242 None:intergenic 35.0%
!! TTCTTTGTTCTTTTGGGTGG+TGG + chr1_3:68053071-68053090 None:intergenic 40.0%
GAAAATTGTGTGGATGGTGA+AGG + chr1_3:68053151-68053170 None:intergenic 40.0%
!! TGGTTTGTTGATTTGATCGC+CGG + chr1_3:68053198-68053217 None:intergenic 40.0%
GCGATCAAATCAACAAACCA+TGG - chr1_3:68053198-68053217 Msa0136220:three_prime_UTR 40.0%
CTAATGCCGAAACCTAAGAT+TGG + chr1_3:68054075-68054094 None:intergenic 40.0%
!! TTCGGCATTAGAGTGTTTTG+AGG - chr1_3:68054084-68054103 Msa0136220:intron 40.0%
! TGGCCTTAAAATTGCTGTGT+GGG + chr1_3:68054311-68054330 None:intergenic 40.0%
! ATGGCCTTAAAATTGCTGTG+TGG + chr1_3:68054312-68054331 None:intergenic 40.0%
!!! ACACAGCAATTTTAAGGCCA+TGG - chr1_3:68054311-68054330 Msa0136220:intron 40.0%
TAAGGCCATGGTATACAGAT+TGG - chr1_3:68054323-68054342 Msa0136220:intron 40.0%
AAGGCCATGGTATACAGATT+GGG - chr1_3:68054324-68054343 Msa0136220:intron 40.0%
CAAACCCAATCTGTATACCA+TGG + chr1_3:68054331-68054350 None:intergenic 40.0%
GCAGTCACAAACTACATTGT+CGG + chr1_3:68054501-68054520 None:intergenic 40.0%
!! TTCTTTGTTCTTTTGGGTGG+TGG + chr1_3:68053071-68053090 None:intergenic 40.0%
GAAAATTGTGTGGATGGTGA+AGG + chr1_3:68053151-68053170 None:intergenic 40.0%
!! TGGTTTGTTGATTTGATCGC+CGG + chr1_3:68053198-68053217 None:intergenic 40.0%
GCGATCAAATCAACAAACCA+TGG - chr1_3:68053198-68053217 Msa0136220:three_prime_UTR 40.0%
CTAATGCCGAAACCTAAGAT+TGG + chr1_3:68054075-68054094 None:intergenic 40.0%
!! TTCGGCATTAGAGTGTTTTG+AGG - chr1_3:68054084-68054103 Msa0136220:intron 40.0%
! TGGCCTTAAAATTGCTGTGT+GGG + chr1_3:68054311-68054330 None:intergenic 40.0%
! ATGGCCTTAAAATTGCTGTG+TGG + chr1_3:68054312-68054331 None:intergenic 40.0%
!!! ACACAGCAATTTTAAGGCCA+TGG - chr1_3:68054311-68054330 Msa0136220:intron 40.0%
TAAGGCCATGGTATACAGAT+TGG - chr1_3:68054323-68054342 Msa0136220:intron 40.0%
AAGGCCATGGTATACAGATT+GGG - chr1_3:68054324-68054343 Msa0136220:intron 40.0%
CAAACCCAATCTGTATACCA+TGG + chr1_3:68054331-68054350 None:intergenic 40.0%
GCAGTCACAAACTACATTGT+CGG + chr1_3:68054501-68054520 None:intergenic 40.0%
CAGCTGGAAAATTGTGTGGA+TGG + chr1_3:68053157-68053176 None:intergenic 45.0%
TCTGCAGCTGGAAAATTGTG+TGG + chr1_3:68053161-68053180 None:intergenic 45.0%
CCAAACATTTGAGTGTCCCA+TGG + chr1_3:68053218-68053237 None:intergenic 45.0%
CCATGGGACACTCAAATGTT+TGG - chr1_3:68053215-68053234 Msa0136220:three_prime_UTR 45.0%
! ATAACTTGTAGCCGTGTGTG+CGG - chr1_3:68053846-68053865 Msa0136220:intron 45.0%
CAATTTCCTTGGCATTGCTG+TGG + chr1_3:68053926-68053945 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG - chr1_3:68053924-68053943 Msa0136220:intron 45.0%
TTCTTTGCCCAGCCAATCTT+AGG - chr1_3:68054060-68054079 Msa0136220:intron 45.0%
!!! GGCATTAGAGTGTTTTGAGG+TGG - chr1_3:68054087-68054106 Msa0136220:intron 45.0%
! TGCCCCACACAGCAATTTTA+AGG - chr1_3:68054305-68054324 Msa0136220:intron 45.0%
GGCCTTAAAATTGCTGTGTG+GGG + chr1_3:68054310-68054329 None:intergenic 45.0%
CAGCTGGAAAATTGTGTGGA+TGG + chr1_3:68053157-68053176 None:intergenic 45.0%
TCTGCAGCTGGAAAATTGTG+TGG + chr1_3:68053161-68053180 None:intergenic 45.0%
CCAAACATTTGAGTGTCCCA+TGG + chr1_3:68053218-68053237 None:intergenic 45.0%
CCATGGGACACTCAAATGTT+TGG - chr1_3:68053215-68053234 Msa0136220:three_prime_UTR 45.0%
! ATAACTTGTAGCCGTGTGTG+CGG - chr1_3:68053846-68053865 Msa0136220:intron 45.0%
CAATTTCCTTGGCATTGCTG+TGG + chr1_3:68053926-68053945 None:intergenic 45.0%
CACAGCAATGCCAAGGAAAT+TGG - chr1_3:68053924-68053943 Msa0136220:intron 45.0%
TTCTTTGCCCAGCCAATCTT+AGG - chr1_3:68054060-68054079 Msa0136220:intron 45.0%
!!! GGCATTAGAGTGTTTTGAGG+TGG - chr1_3:68054087-68054106 Msa0136220:intron 45.0%
! TGCCCCACACAGCAATTTTA+AGG - chr1_3:68054305-68054324 Msa0136220:intron 45.0%
GGCCTTAAAATTGCTGTGTG+GGG + chr1_3:68054310-68054329 None:intergenic 45.0%
AGACTCAAATGCTCTGCAGC+TGG + chr1_3:68053173-68053192 None:intergenic 50.0%
! GCAGAGCATTTGAGTCTCAC+CGG - chr1_3:68053176-68053195 Msa0136220:three_prime_UTR 50.0%
AACTCTCACCCCAAGACCTA+TGG - chr1_3:68053238-68053257 Msa0136220:three_prime_UTR 50.0%
AACCAGGACCATAGGTCTTG+GGG + chr1_3:68053249-68053268 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG + chr1_3:68053250-68053269 None:intergenic 50.0%
ATATACCAAGTGCGGGAACC+AGG + chr1_3:68053265-68053284 None:intergenic 50.0%
! TAACTTGTAGCCGTGTGTGC+GGG - chr1_3:68053847-68053866 Msa0136220:intron 50.0%
CATGTGAATTCCCGCACACA+CGG + chr1_3:68053860-68053879 None:intergenic 50.0%
!! AGTATGGCCTTATGTGCTGC+AGG - chr1_3:68053892-68053911 Msa0136220:intron 50.0%
AGCATTGCCTGCAGCACATA+AGG + chr1_3:68053902-68053921 None:intergenic 50.0%
ATGCTTCCACAGCAATGCCA+AGG - chr1_3:68053917-68053936 Msa0136220:intron 50.0%
GCCCAGCCAATCTTAGGTTT+CGG - chr1_3:68054066-68054085 Msa0136220:intron 50.0%
! GCCGAAACCTAAGATTGGCT+GGG + chr1_3:68054070-68054089 None:intergenic 50.0%
TGCCGAAACCTAAGATTGGC+TGG + chr1_3:68054071-68054090 None:intergenic 50.0%
AGACTCAAATGCTCTGCAGC+TGG + chr1_3:68053173-68053192 None:intergenic 50.0%
! GCAGAGCATTTGAGTCTCAC+CGG - chr1_3:68053176-68053195 Msa0136220:three_prime_UTR 50.0%
AACTCTCACCCCAAGACCTA+TGG - chr1_3:68053238-68053257 Msa0136220:three_prime_UTR 50.0%
AACCAGGACCATAGGTCTTG+GGG + chr1_3:68053249-68053268 None:intergenic 50.0%
GAACCAGGACCATAGGTCTT+GGG + chr1_3:68053250-68053269 None:intergenic 50.0%
ATATACCAAGTGCGGGAACC+AGG + chr1_3:68053265-68053284 None:intergenic 50.0%
! TAACTTGTAGCCGTGTGTGC+GGG - chr1_3:68053847-68053866 Msa0136220:intron 50.0%
CATGTGAATTCCCGCACACA+CGG + chr1_3:68053860-68053879 None:intergenic 50.0%
!! AGTATGGCCTTATGTGCTGC+AGG - chr1_3:68053892-68053911 Msa0136220:intron 50.0%
AGCATTGCCTGCAGCACATA+AGG + chr1_3:68053902-68053921 None:intergenic 50.0%
ATGCTTCCACAGCAATGCCA+AGG - chr1_3:68053917-68053936 Msa0136220:intron 50.0%
GCCCAGCCAATCTTAGGTTT+CGG - chr1_3:68054066-68054085 Msa0136220:intron 50.0%
! GCCGAAACCTAAGATTGGCT+GGG + chr1_3:68054070-68054089 None:intergenic 50.0%
TGCCGAAACCTAAGATTGGC+TGG + chr1_3:68054071-68054090 None:intergenic 50.0%
GGAACCAGGACCATAGGTCT+TGG + chr1_3:68053251-68053270 None:intergenic 55.0%
CGTGTGTGCGGGAATTCACA+TGG - chr1_3:68053858-68053877 Msa0136220:intron 55.0%
GGAACCAGGACCATAGGTCT+TGG + chr1_3:68053251-68053270 None:intergenic 55.0%
CGTGTGTGCGGGAATTCACA+TGG - chr1_3:68053858-68053877 Msa0136220:intron 55.0%
CACCCCAAGACCTATGGTCC+TGG - chr1_3:68053244-68053263 Msa0136220:three_prime_UTR 60.0%
AGTGCGGGAACCAGGACCAT+AGG + chr1_3:68053257-68053276 None:intergenic 60.0%
ATGGTCCTGGTTCCCGCACT+TGG - chr1_3:68053257-68053276 Msa0136220:three_prime_UTR 60.0%
CACCCCAAGACCTATGGTCC+TGG - chr1_3:68053244-68053263 Msa0136220:three_prime_UTR 60.0%
AGTGCGGGAACCAGGACCAT+AGG + chr1_3:68053257-68053276 None:intergenic 60.0%
ATGGTCCTGGTTCCCGCACT+TGG - chr1_3:68053257-68053276 Msa0136220:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68053064 68054553 68053064 ID=Msa0136220;Name=Msa0136220
chr1_3 mRNA 68053064 68054553 68053064 ID=Msa0136220-mRNA-1;Parent=Msa0136220;Name=Msa0136220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=253|1|1|1|0.5|0.33|3|472|33
chr1_3 exon 68053064 68053575 68053064 ID=Msa0136220-mRNA-1:exon:23463;Parent=Msa0136220-mRNA-1
chr1_3 exon 68053661 68053760 68053661 ID=Msa0136220-mRNA-1:exon:23462;Parent=Msa0136220-mRNA-1
chr1_3 exon 68054339 68054553 68054339 ID=Msa0136220-mRNA-1:exon:23461;Parent=Msa0136220-mRNA-1
chr1_3 five_prime_UTR 68054339 68054553 68054339 ID=Msa0136220-mRNA-1:five_prime_utr;Parent=Msa0136220-mRNA-1
chr1_3 five_prime_UTR 68053723 68053760 68053723 ID=Msa0136220-mRNA-1:five_prime_utr;Parent=Msa0136220-mRNA-1
chr1_3 CDS 68053661 68053722 68053661 ID=Msa0136220-mRNA-1:cds;Parent=Msa0136220-mRNA-1
chr1_3 CDS 68053536 68053575 68053536 ID=Msa0136220-mRNA-1:cds;Parent=Msa0136220-mRNA-1
chr1_3 three_prime_UTR 68053064 68053535 68053064 ID=Msa0136220-mRNA-1:three_prime_utr;Parent=Msa0136220-mRNA-1
Gene Sequence

>Msa0136220

ATGGCCTTATGTGCTGCAGGCAATGCTTCCACAGCAATGCCAAGGAAATTGGCTTCATTAAGTACCGTTGAAGAGCTATTTTCTTTGCCCAGCCAATCTTAG
Protein sequence

>Msa0136220

MALCAAGNASTAMPRKLASLSTVEELFSLPSQS*