AlfalfaGEDB | Msa0092470

M. sativa cultivar zhongmu-4 / Msa0092470

KEGG: K13414|K20605
GO: GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0092470	sp\|Q39008\|M3K1_ARATH	59.162	382	135	8	333	704	221	591	2.68e-138	422
Msa0092470	sp\|Q39008\|M3K1_ARATH	68.812	202	58	2	4	200	335	536	2.92e-84	281

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0092470	G7J3N7	87.831	189	19	1	16	200	284	472	4.29e-106	343
Msa0092470	G7J3N7	89.630	540	28	4	202	717	5	540	0.0	888

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0092470	Msa0135330	0.800337	1.511085e-48	-8.615850e-47
Msa0092470	Msa0145090	0.806228	9.110444e-50	-8.615850e-47
Msa0092470	Msa0184750	0.800525	1.383601e-48	-8.615850e-47
Msa0092470	Msa0269480	0.841128	5.732117e-58	-8.615850e-47
Msa0092470	Msa0417760	0.801578	8.429888e-49	-8.615850e-47
Msa0092470	Msa0534000	0.808823	2.563236e-50	-8.615850e-47
Msa0092470	Msa0590010	0.816367	5.736588e-52	-8.615850e-47
Msa0092470	Msa0839530	0.810490	1.123505e-50	-8.615850e-47
Msa0092470	Msa1222400	0.803940	2.742015e-49	-8.615850e-47
Msa0092470	Msa1264660	0.802867	4.575166e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0092470	MtrunA17_Chr3g0115171	89.630	540	28	4	202	717	18	553	0.0	888
Msa0092470	MtrunA17_Chr3g0115171	87.831	189	19	1	16	200	297	485	2.19e-109	343
Msa0092470	MtrunA17_Chr5g0424651	59.655	580	141	11	197	702	16	576	0.0	617
Msa0092470	MtrunA17_Chr5g0424651	76.733	202	43	1	4	201	323	524	7.25e-102	324
Msa0092470	MtrunA17_Chr5g0424501	58.642	486	125	6	247	702	8	447	7.79e-179	518
Msa0092470	MtrunA17_Chr5g0424501	76.617	201	43	1	4	200	194	394	3.56e-101	318
Msa0092470	MtrunA17_Chr5g0424581	72.372	333	77	2	370	702	63	380	4.12e-165	480
Msa0092470	MtrunA17_Chr5g0424581	76.733	202	43	1	4	201	127	328	1.55e-103	322
Msa0092470	MtrunA17_Chr4g0051191	74.721	269	66	2	446	713	162	429	2.12e-138	413
Msa0092470	MtrunA17_Chr4g0051191	69.802	202	56	2	4	200	165	366	1.45e-88	284

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0092470	AT4G08500.1	59.162	382	135	8	333	704	221	591	2.73e-139	422
Msa0092470	AT4G08500.1	68.812	202	58	2	4	200	335	536	2.97e-85	281
Msa0092470	AT4G08480.1	55.699	386	142	8	332	703	388	758	6.09e-131	406
Msa0092470	AT4G08480.1	64.356	202	67	2	4	200	503	704	1.83e-76	262
Msa0092470	AT4G08480.1	44.554	101	36	4	208	294	123	217	2.09e-11	67.8
Msa0092470	AT4G08470.1	61.168	291	107	3	414	700	269	557	6.43e-115	358
Msa0092470	AT4G08470.1	62.871	202	70	2	4	200	305	506	5.23e-77	258
Msa0092470	AT4G08470.3	65.625	256	87	1	446	700	220	475	1.17e-114	354
Msa0092470	AT4G08470.3	62.871	202	70	2	4	200	223	424	5.07e-77	256
Msa0092470	AT4G12020.2	53.581	377	148	10	333	704	1527	1881	6.66e-102	344

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Find 223 sgRNAs with CRISPR-Local

Find 926 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAAGTGTCTATCAAC+TGG	0.433038	1_2:+74939290	Msa0092470:CDS
AAAACAAAGTGTCTCTCAAC+TGG	0.413057	1_2:+74930899	Msa0092470:CDS
AAAATGCAGAGCTTAGATGT+TGG	0.577212	1_2:+74944430	Msa0092470:three_prime_UTR
AAACGCTTAATTCTCCCGTT+TGG	0.367480	1_2:-74938455	None:intergenic
AAAGGAAAGAACAATGGTTA+TGG	0.453131	1_2:+74933823	Msa0092470:CDS
AAAGGACAAATCATCATCTG+TGG	0.605692	1_2:-74944337	None:intergenic
AACATTCTGGATGGCCCCTG+AGG	0.614718	1_2:+74933657	Msa0092470:CDS
AACGATGTTAAATCATGCAA+GGG	0.593956	1_2:+74941574	Msa0092470:CDS
AACTCCAAACATCAGCTTGA+AGG	0.428588	1_2:-74933846	None:intergenic
AAGAAGGTGAGGAAGTTGGT+AGG	0.489872	1_2:+74929401	Msa0092470:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAGTGTAAAATTACACC+TGG	+	chr1_2:74929563-74929582	Msa0092470:five_prime_UTR	30.0%
GTAGTTGAGGAATTTATGAT+TGG	+	chr1_2:74929838-74929857	Msa0092470:intron	30.0%
GTTCATTTCAAGAATATTGC+TGG	+	chr1_2:74929869-74929888	Msa0092470:intron	30.0%
ATTTCAAGAATATTGCTGGA+TGG	+	chr1_2:74929873-74929892	Msa0092470:intron	30.0%
TTTCAAGAATATTGCTGGAT+GGG	+	chr1_2:74929874-74929893	Msa0092470:intron	30.0%
TGCTATCTCTTTCAATATAG+TGG	+	chr1_2:74929930-74929949	Msa0092470:intron	30.0%
TTACTAACATTCAATCTCTC+CGG	+	chr1_2:74930003-74930022	Msa0092470:intron	30.0%
CAAAATTTAAGGGTAGCTAA+CGG	+	chr1_2:74930037-74930056	Msa0092470:intron	30.0%
TCGTATTGTTCATTCTGATT+AGG	+	chr1_2:74930428-74930447	Msa0092470:intron	30.0%
CTAATACACAATTCAGTTAG+GGG	-	chr1_2:74930475-74930494	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	74929353	74944643	74929353	ID=Msa0092470;Name=Msa0092470
chr1_2	mRNA	74929353	74944643	74929353	ID=Msa0092470-mRNA-1;Parent=Msa0092470;Name=Msa0092470-mRNA-1;_AED=0.16;_eAED=0.16;_QI=202\|0.73\|0.75\|1\|0.63\|0.6\|20\|437\|717
chr1_2	exon	74929353	74929366	74929353	ID=Msa0092470-mRNA-1:exon:26381;Parent=Msa0092470-mRNA-1
chr1_2	exon	74929398	74929650	74929398	ID=Msa0092470-mRNA-1:exon:26382;Parent=Msa0092470-mRNA-1
chr1_2	exon	74930842	74930926	74930842	ID=Msa0092470-mRNA-1:exon:26383;Parent=Msa0092470-mRNA-1
chr1_2	exon	74931145	74931207	74931145	ID=Msa0092470-mRNA-1:exon:26384;Parent=Msa0092470-mRNA-1
chr1_2	exon	74932645	74932790	74932645	ID=Msa0092470-mRNA-1:exon:26385;Parent=Msa0092470-mRNA-1
chr1_2	exon	74933411	74933483	74933411	ID=Msa0092470-mRNA-1:exon:26386;Parent=Msa0092470-mRNA-1
chr1_2	exon	74933619	74933678	74933619	ID=Msa0092470-mRNA-1:exon:26387;Parent=Msa0092470-mRNA-1
chr1_2	exon	74933817	74933927	74933817	ID=Msa0092470-mRNA-1:exon:26388;Parent=Msa0092470-mRNA-1
chr1_2	exon	74937696	74937858	74937696	ID=Msa0092470-mRNA-1:exon:26389;Parent=Msa0092470-mRNA-1
chr1_2	exon	74937889	74938032	74937889	ID=Msa0092470-mRNA-1:exon:26390;Parent=Msa0092470-mRNA-1
chr1_2	exon	74938057	74938302	74938057	ID=Msa0092470-mRNA-1:exon:26391;Parent=Msa0092470-mRNA-1
chr1_2	exon	74938312	74938555	74938312	ID=Msa0092470-mRNA-1:exon:26392;Parent=Msa0092470-mRNA-1
chr1_2	exon	74939233	74939317	74939233	ID=Msa0092470-mRNA-1:exon:26393;Parent=Msa0092470-mRNA-1
chr1_2	exon	74939519	74939581	74939519	ID=Msa0092470-mRNA-1:exon:26394;Parent=Msa0092470-mRNA-1
chr1_2	exon	74940437	74940594	74940437	ID=Msa0092470-mRNA-1:exon:26395;Parent=Msa0092470-mRNA-1
chr1_2	exon	74941354	74941426	74941354	ID=Msa0092470-mRNA-1:exon:26396;Parent=Msa0092470-mRNA-1
chr1_2	exon	74941562	74941621	74941562	ID=Msa0092470-mRNA-1:exon:26397;Parent=Msa0092470-mRNA-1
chr1_2	exon	74942922	74943032	74942922	ID=Msa0092470-mRNA-1:exon:26398;Parent=Msa0092470-mRNA-1
chr1_2	exon	74943485	74943722	74943485	ID=Msa0092470-mRNA-1:exon:26399;Parent=Msa0092470-mRNA-1
chr1_2	exon	74944241	74944643	74944241	ID=Msa0092470-mRNA-1:exon:26400;Parent=Msa0092470-mRNA-1
chr1_2	five_prime_UTR	74929353	74929366	74929353	ID=Msa0092470-mRNA-1:five_prime_utr;Parent=Msa0092470-mRNA-1
chr1_2	five_prime_UTR	74929398	74929585	74929398	ID=Msa0092470-mRNA-1:five_prime_utr;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74929586	74929650	74929586	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74930842	74930926	74930842	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74931145	74931207	74931145	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74932645	74932790	74932645	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74933411	74933483	74933411	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74933619	74933678	74933619	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74933817	74933927	74933817	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74937696	74937858	74937696	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74937889	74938032	74937889	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74938057	74938302	74938057	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74938312	74938555	74938312	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74939233	74939317	74939233	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74939519	74939581	74939519	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74940437	74940594	74940437	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74941354	74941426	74941354	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74941562	74941621	74941562	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74942922	74943032	74942922	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	CDS	74943485	74943688	74943485	ID=Msa0092470-mRNA-1:cds;Parent=Msa0092470-mRNA-1
chr1_2	three_prime_UTR	74943689	74943722	74943689	ID=Msa0092470-mRNA-1:three_prime_utr;Parent=Msa0092470-mRNA-1
chr1_2	three_prime_UTR	74944241	74944643	74944241	ID=Msa0092470-mRNA-1:three_prime_utr;Parent=Msa0092470-mRNA-1

Gene Sequence

>Msa0092470

ATGCTGGCAAAAGGCAACTTTTTGGGGGGTGGATCGTTTGGATCTGTCTATGAAGGAATTTCTAACGATGGATTCTTTTTTGATGTAAAAGAAGTTTCATTGCTTGATCAAAGGGATCGGGGAAAACAAAGTGTCTCTCAACTGGAGCAGGAAATCGCACTTTTGAGTCGTTTTAAACATGACAATATAGTTCAATACTATGGTACTGAAATGCTGTATATATTCATTGAACTTGTAACCAAAGGTTCTCTTAGAAGCCTATATCAGAGGTACACTCTTCAAGATTCCCAAGTATCTGTCTATACAAGACAGATTCTGCATGGTTTGAAATATCTTCATGATCAAAATGTGGTTCACAGGGATATCAAATGTGCAAATATATTGGTGCATGCAAGTGGATCTGTCAAACTCGCCGATTTTGAGTTAACAAAGGAAACCAAATTGAATGATGTTAAATCATGCAAGGGGACAACATTCTGGATGGCCCCTGAGGTCGTGAAAGGAAAGAACAATGGTTATGGCCTTCAAGCTGATGTTTGGAGTTTGGGATGCACTGTGCTGGAGATGCTAACTGGCCAAATTCCATACTCCAATTTGGAATGTAAGCTTCGAAGAAAACCTAAACTCGAGCGTCGCAATGCTCAAAAGTACACCGATTACGACGCTGGATCATCTTCATCTTCTTTCGATGACTCTTCTGGTTCACTTTACACTCGTTCTATGGAGTTATACGACCGTACTAGCTTCCGTATTGAAGGAGTTGATGGACTTTCTGGACCCGAGGATTTTGCTATCCCTGCTGCTGCTTGGGAAGCTATGAAGATTCGATCCAATTCTGATGTTCTTCCCACTTTGAAGCTTGATGAGCTTGATTTGAAGGAGAAGAAGGTGAATGATGTGAGAGAATTAGATAGGGATATGGTTAGGGTTAGGGTTAAAGATGAGGTTGATGACAGTGAGAGTGTTGTTGTTGTTAGTAGTAGTAGTAGTAGTGGTATTAATGGAACTCGTCCACCGATGATTAAGCCGCCACCTGGGATGAGAGTTCCGGTGGTTGTTGATAACACGTGTTCCACTTGGGATCTTTTGAAGGATTTGGCTCCGGTTGGGGAAGGGGAAGAAGAGGGTTTTGACCGGGAAGTGGAGGAGAGTGAAGTTGGTAGAGAAGAGGAGGTGGTGGATAATGAGGCGAGGATTGATGAGATTGTTGCTGGTCTTATGGAATCGAGTTTGTTTAGTGATTCGAATGAAGATGATTCTTCTAGTACTGCTATGGAACCTAGATCGAATAATGTATCTCCAAACGGGAGAATTAAGCGTTTTATTACGCCTGGAAGCTGGCAAAAGGGTGGTTTTTTGGGGGGTGGTTCGTTTGGATCTGTCTATGAAGGAATTTCTGACGATGGATTCTTTTTTGCTGTAAAAGAAGTTTCCTTGCTTGATCAAGGGGAGCAGGGAAAACAAAGTGTCTATCAACTGGAGCAGGAAATTGCACTTTTGAGTCGTTTTGAACATGATAATATAGTTCAATACTATGGCACTGAAATGGATGAATCTAAGCTTCATATCTTTATTGAGCTTGTAACCAAAGGTTCTCTTAGAAGCCTCTATCAGAGGTATACTCTTCGAGATTCCCAAGTAGCTGCCTATACAAGACAGATTCTGCATGGTTTGAAGTACCTTCATGATCAAAATGTGGTTCACAGGGATATCAAATGTGCAAATATATTGGTGCATGCAAGTGGGTCTGTCAAACTTGCAGATTTTGGGTTAGCAAAGGCAACCAAATTGAACGATGTTAAATCATGCAAGGGAACAGCATTCTGGATGGCCCCTGAGGTTGTGAGAGGAAAGAACAAAGGTTACGGGCTTCCAGCCGATATTTGGAGTCTGGGATGCACTGTTCTGGAGATGCTAACGGGCCAAATTCCGTACTCCAATTTGGAACCTATGCAGGCATTATTTAGAATAGGAAAAGGTGAGCCACCTCCTATTCCTGATAGTCTTTCAAGAGATGCAAAAGATTTTATCATGCAGTGCCTTCAAGTTAATCCTGATGATCGTTCTACTGCTGCTCAACTCTTAAACCATCCATTTCTCCAAAGGCCACTTTCCCAGTCTTCATCTCCTTACATTCATGGCAGAAGGGGTTAA

Protein sequence

>Msa0092470

MLAKGNFLGGGSFGSVYEGISNDGFFFDVKEVSLLDQRDRGKQSVSQLEQEIALLSRFKHDNIVQYYGTEMLYIFIELVTKGSLRSLYQRYTLQDSQVSVYTRQILHGLKYLHDQNVVHRDIKCANILVHASGSVKLADFELTKETKLNDVKSCKGTTFWMAPEVVKGKNNGYGLQADVWSLGCTVLEMLTGQIPYSNLECKLRRKPKLERRNAQKYTDYDAGSSSSSFDDSSGSLYTRSMELYDRTSFRIEGVDGLSGPEDFAIPAAAWEAMKIRSNSDVLPTLKLDELDLKEKKVNDVRELDRDMVRVRVKDEVDDSESVVVVSSSSSSGINGTRPPMIKPPPGMRVPVVVDNTCSTWDLLKDLAPVGEGEEEGFDREVEESEVGREEEVVDNEARIDEIVAGLMESSLFSDSNEDDSSSTAMEPRSNNVSPNGRIKRFITPGSWQKGGFLGGGSFGSVYEGISDDGFFFAVKEVSLLDQGEQGKQSVYQLEQEIALLSRFEHDNIVQYYGTEMDESKLHIFIELVTKGSLRSLYQRYTLRDSQVAAYTRQILHGLKYLHDQNVVHRDIKCANILVHASGSVKLADFGLAKATKLNDVKSCKGTAFWMAPEVVRGKNKGYGLPADIWSLGCTVLEMLTGQIPYSNLEPMQALFRIGKGEPPPIPDSLSRDAKDFIMQCLQVNPDDRSTAAQLLNHPFLQRPLSQSSSPYIHGRRG*