AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135330 MtrunA17_Chr1g0205741 97.196 321 9 0 1 321 1 321 0.0 649
Msa0135330 MtrunA17_Chr4g0044931 48.649 185 90 4 25 207 4 185 2.36e-50 166
Msa0135330 MtrunA17_Chr4g0044941 46.341 123 62 1 204 322 8 130 1.17e-32 122
Msa0135330 MtrunA17_Chr6g0479311 33.333 258 154 12 28 280 29 273 5.79e-28 113
Msa0135330 MtrunA17_Chr6g0487571 29.936 314 189 14 2 294 4 307 1.38e-21 95.5
Msa0135330 MtrunA17_Chr8g0341151 28.349 321 184 16 2 298 4 302 5.64e-21 93.6
Msa0135330 MtrunA17_Chr8g0340931 27.059 340 215 16 3 322 4 330 1.36e-20 92.8
Msa0135330 MtrunA17_Chr8g0340871 27.859 341 213 17 1 322 10 336 5.93e-20 90.9
Msa0135330 MtrunA17_Chr8g0340911 27.246 345 207 17 1 322 8 331 1.89e-19 89.4
Msa0135330 MtrunA17_Chr1g0181731 33.600 250 134 14 18 248 21 257 2.77e-19 88.6
Msa0135330 MtrunA17_Chr7g0263591 33.469 245 129 14 24 248 31 261 3.43e-19 88.6
Msa0135330 MtrunA17_Chr8g0341231 27.541 305 175 16 43 322 73 356 4.30e-19 87.8
Msa0135330 MtrunA17_Chr3g0123141 29.098 244 135 12 28 248 28 256 4.70e-19 88.2
Msa0135330 MtrunA17_Chr6g0487561 28.526 312 190 14 3 294 18 316 2.66e-18 84.7
Msa0135330 MtrunA17_Chr2g0330591 27.885 312 182 18 8 294 11 304 3.27e-18 85.5
Msa0135330 MtrunA17_Chr8g0341351 26.490 302 173 16 19 293 37 316 5.23e-18 84.0
Msa0135330 MtrunA17_Chr8g0340891 27.027 333 207 17 1 314 8 323 7.54e-18 84.3
Msa0135330 MtrunA17_Chr8g0340941 29.097 299 169 17 25 298 26 306 1.41e-17 83.2
Msa0135330 MtrunA17_Chr8g0340881 26.420 352 204 18 1 322 8 334 1.23e-16 80.9
Msa0135330 MtrunA17_Chr5g0411631 27.711 249 152 8 68 307 81 310 2.69e-16 79.7
Msa0135330 MtrunA17_Chr5g0422481 28.869 336 204 14 7 311 17 348 4.83e-15 75.9
Msa0135330 MtrunA17_Chr7g0236881 26.144 306 194 13 4 294 9 297 1.32e-13 71.6
Msa0135330 MtrunA17_Chr2g0282601 26.888 331 202 17 3 304 1 320 3.35e-13 70.1
Msa0135330 MtrunA17_Chr8g0340961 24.468 282 165 13 42 306 51 301 1.16e-11 64.7
Msa0135330 MtrunA17_Chr5g0423881 28.767 292 168 15 28 294 66 342 7.12e-11 62.8
Msa0135330 MtrunA17_Chr1g0198681 23.701 308 202 12 26 306 38 339 7.23e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135330 AT3G05990.1 57.705 305 119 4 25 321 27 329 7.07e-121 357
Msa0135330 AT3G19230.1 45.367 313 162 5 17 322 14 324 7.51e-95 291
Msa0135330 AT3G46270.1 31.786 280 170 12 24 293 28 296 2.09e-26 108
Msa0135330 AT3G46270.2 31.786 280 170 12 24 293 36 304 2.13e-26 108
Msa0135330 AT2G14510.1 29.355 310 191 14 5 297 7 305 2.09e-24 104
Msa0135330 AT2G14510.2 29.355 310 191 14 5 297 15 313 2.44e-24 103
Msa0135330 AT3G46260.1 28.571 287 181 13 24 296 27 303 7.66e-24 101
Msa0135330 AT2G14440.1 30.515 272 165 12 38 297 47 306 9.29e-24 102
Msa0135330 AT3G46280.1 28.146 302 195 12 1 293 2 290 9.55e-24 101
Msa0135330 AT4G29450.1 29.304 273 160 12 28 280 30 289 1.64e-21 95.9
Msa0135330 AT1G51890.1 26.948 308 198 11 1 294 1 295 3.31e-21 94.7
Msa0135330 AT1G51890.3 26.948 308 198 11 1 294 1 295 3.53e-21 94.7
Msa0135330 AT1G51890.2 28.070 285 177 12 25 294 24 295 3.71e-21 94.7
Msa0135330 AT2G37050.3 32.129 249 145 12 15 248 19 258 5.01e-21 94.4
Msa0135330 AT2G37050.1 32.129 249 145 12 15 248 19 258 5.45e-21 94.4
Msa0135330 AT2G37050.4 32.129 249 145 12 15 248 19 258 6.85e-21 94.0
Msa0135330 AT2G37050.2 32.129 249 145 12 15 248 19 258 6.91e-21 94.0
Msa0135330 AT1G51910.1 27.124 306 195 12 5 294 7 300 2.48e-20 92.4
Msa0135330 AT3G46350.1 30.233 258 160 10 46 294 57 303 7.17e-20 90.1
Msa0135330 AT1G24485.4 28.571 294 177 16 28 310 31 302 8.60e-20 89.7
Msa0135330 AT1G24485.2 27.273 319 201 16 1 310 6 302 9.36e-20 90.1
Msa0135330 AT1G24485.1 27.273 319 201 16 1 310 6 302 1.00e-19 89.7
Msa0135330 AT1G67720.1 30.041 243 145 10 28 254 29 262 1.06e-19 90.5
Msa0135330 AT1G24485.3 28.082 292 181 15 28 310 31 302 1.36e-19 89.7
Msa0135330 AT1G51850.2 27.444 266 167 10 42 298 48 296 1.07e-18 87.4
Msa0135330 AT4G29180.1 29.195 298 175 15 3 280 8 289 2.79e-18 86.3
Msa0135330 AT4G29180.2 29.195 298 175 15 3 280 8 289 2.90e-18 86.3
Msa0135330 AT4G29180.3 29.195 298 175 15 3 280 8 289 2.98e-18 86.3
Msa0135330 AT1G51805.1 26.804 291 181 12 19 294 20 293 3.14e-18 85.9
Msa0135330 AT1G05700.2 30.584 291 167 17 25 295 30 305 4.43e-18 85.5
Msa0135330 AT1G05700.1 30.584 291 167 17 25 295 30 305 7.14e-18 85.1
Msa0135330 AT4G29990.2 28.136 295 171 16 25 295 26 303 7.17e-18 84.7
Msa0135330 AT4G29990.1 28.136 295 171 16 25 295 26 303 8.68e-18 84.7
Msa0135330 AT1G51820.1 26.718 262 166 10 42 294 48 292 5.33e-17 82.4
Msa0135330 AT2G04300.2 26.364 330 211 15 2 311 7 324 2.25e-16 80.5
Msa0135330 AT2G19230.2 28.520 277 160 15 25 280 29 288 3.82e-16 79.7
Msa0135330 AT2G19230.1 28.520 277 160 15 25 280 29 288 3.90e-16 79.7
Msa0135330 AT2G04300.1 26.452 310 200 13 2 294 7 305 4.03e-16 79.7
Msa0135330 AT1G51860.1 27.687 307 192 14 5 294 7 300 1.61e-15 77.8
Msa0135330 AT1G51850.1 26.692 266 149 10 42 298 48 276 1.75e-15 77.8
Msa0135330 AT5G59670.2 26.829 287 182 13 23 294 25 298 1.96e-15 77.8
Msa0135330 AT5G59670.1 26.829 287 182 13 23 294 25 298 1.96e-15 77.8
Msa0135330 AT5G48740.1 28.664 307 184 13 4 293 1 289 2.23e-15 77.4
Msa0135330 AT3G21340.2 25.962 312 196 14 8 299 13 309 2.46e-15 77.0
Msa0135330 AT3G21340.1 25.962 312 196 14 8 299 13 309 2.80e-15 77.0
Msa0135330 AT1G51880.3 25.595 336 221 14 2 320 21 344 4.95e-15 76.3
Msa0135330 AT1G51880.1 25.602 332 218 14 6 320 7 326 5.18e-15 76.3
Msa0135330 AT1G51880.2 25.595 336 221 14 2 320 21 344 7.19e-15 75.9
Msa0135330 AT1G49100.1 26.871 294 182 12 23 298 27 305 1.03e-14 75.5
Msa0135330 AT3G46400.1 28.774 212 133 9 43 248 52 251 5.00e-14 73.2
Msa0135330 AT2G29000.1 28.079 203 122 8 23 216 27 214 5.21e-14 73.2
Msa0135330 AT2G19210.1 26.885 305 175 16 1 280 7 288 6.33e-14 73.2
Msa0135330 AT3G46340.1 31.169 231 132 12 2 218 6 223 3.05e-13 70.9
Msa0135330 AT3G46240.1 27.046 281 164 11 25 299 5 250 1.79e-12 68.2
Msa0135330 AT1G51805.2 23.779 307 175 14 4 294 6 269 4.20e-11 64.3
Msa0135330 AT1G07560.1 28.019 207 125 10 24 219 28 221 4.83e-11 64.3
Msa0135330 AT3G46370.2 25.110 227 155 8 79 300 2 218 7.44e-11 63.5
Msa0135330 AT3G46370.1 25.110 227 155 8 79 300 2 218 7.44e-11 63.5

Find 181 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCAACCAATGGCCTCTTTC+TGG 0.086922 1_3:-66954896 Msa0135330:CDS
TGCTTAGATTGTTCTGATTT+TGG 0.164807 1_3:-66953454 Msa0135330:three_prime_UTR
ACTGAGAAAGTTTCTGTTTC+TGG 0.167891 1_3:-66955145 Msa0135330:CDS
ATGATCAGTTTGACCGTATC+TGG 0.219580 1_3:-66955204 Msa0135330:CDS
AAGTTTCTGTTTCTGGCTTT+TGG 0.221659 1_3:-66955138 Msa0135330:CDS
TTTCAGAAATTTGACAAGTA+TGG 0.245376 1_3:-66954441 Msa0135330:intron
TTGACAAGTATGGATCTTTC+AGG 0.246945 1_3:-66954431 Msa0135330:three_prime_UTR
GACTTCGCCAGCATTAATTA+AGG 0.250148 1_3:+66954827 None:intergenic
GGTGTTGAGGAAAGAGAATT+TGG 0.266738 1_3:+66956483 None:intergenic
GCCATGTACGGTTGTTGATT+TGG 0.295163 1_3:+66956287 None:intergenic
AGGTAACAAGAAATTGTTTC+CGG 0.302226 1_3:+66953551 None:intergenic
TTCCTTGTTGCAGCTGGCTT+GGG 0.313146 1_3:-66954293 Msa0135330:intron
AAAGTATTTGAAACACATTT+AGG 0.317793 1_3:-66955100 Msa0135330:CDS
TATCTTACATATCATTGTAT+AGG 0.318327 1_3:-66953254 None:intergenic
GTATAGAGCGATGTAGTATT+TGG 0.318913 1_3:+66955021 None:intergenic
TTTCCTTGTTGCAGCTGGCT+TGG 0.324854 1_3:-66954294 Msa0135330:intron
TTACAAAACAACAATTTAAC+CGG 0.326698 1_3:-66953703 Msa0135330:three_prime_UTR
CCATGTACGGTTGTTGATTT+GGG 0.332412 1_3:+66956288 None:intergenic
AACTTTCTCAGTGTTTGCTT+TGG 0.333727 1_3:+66955156 None:intergenic
CGTTGGATGGGTTGGGTTGA+AGG 0.339430 1_3:+66956660 None:intergenic
TATCGGGTCGAATAGTTATA+CGG 0.340421 1_3:-66955933 Msa0135330:CDS
GCAGTTGATGAGGAAGCCTT+TGG 0.341686 1_3:+66956327 None:intergenic
GATTTGAATGCAATTGCTTC+AGG 0.342508 1_3:-66954932 Msa0135330:CDS
CAGTTGATGAGGAAGCCTTT+GGG 0.348095 1_3:+66956328 None:intergenic
GGAGTGGGAGGGAGGGAAGA+AGG 0.356615 1_3:+66956510 None:intergenic
GTCTTTAATGTGTTGTCTCT+TGG 0.356810 1_3:-66954807 Msa0135330:three_prime_UTR
TGACCCGTTTGTTTCTGCTT+TGG 0.360641 1_3:-66955906 Msa0135330:CDS
TGTTGATTTGGGTTGTTGTC+AGG 0.364224 1_3:+66956299 None:intergenic
TCCAATCAAGTGGAGGATTT+CGG 0.369878 1_3:+66954639 None:intergenic
TTCAAAGGACAGAACAGAAT+TGG 0.373839 1_3:+66956609 None:intergenic
TAACAACTTTGCTATTGGTT+TGG 0.376028 1_3:-66955840 Msa0135330:CDS
GTGTTTGATCAGATCGTTGA+TGG 0.378868 1_3:-66956058 Msa0135330:CDS
TCCATCTGCTAGTTCATCCT+TGG 0.380544 1_3:-66954853 Msa0135330:three_prime_UTR
CCTGGTTTAATGAGATTTGC+GGG 0.384360 1_3:+66953673 None:intergenic
CTTGGAGTGTACTTTCATAA+TGG 0.387176 1_3:-66953340 Msa0135330:three_prime_UTR
ATATTTATCAACAGAACTTC+CGG 0.389753 1_3:-66953570 Msa0135330:intron
ACTACGGTGGATTATGCTAA+TGG 0.390128 1_3:-66956013 Msa0135330:CDS
TGATCAAACACCGGCGGCTT+AGG 0.393518 1_3:+66956070 None:intergenic
ATCTGATCAATTAGAATCCT+TGG 0.397377 1_3:-66955077 Msa0135330:CDS
TCCGAAATCCTCCACTTGAT+TGG 0.401383 1_3:-66954640 Msa0135330:three_prime_UTR
GGAACAGATTCTTTCAACTT+TGG 0.403558 1_3:-66953316 Msa0135330:three_prime_UTR
ACGCGGGAAACTGTGTTAAC+TGG 0.406704 1_3:+66956569 None:intergenic
GTCTTTATCTCTACCATAGT+AGG 0.408848 1_3:+66953501 None:intergenic
AATAGCAAAGTTGTTAAAAT+CGG 0.415393 1_3:+66955847 None:intergenic
TGTTACCTCCAGCACCTTGA+GGG 0.416538 1_3:-66953536 Msa0135330:three_prime_UTR
TGGAGAAGGAGTGGGAGGGA+GGG 0.417761 1_3:+66956503 None:intergenic
GATTTGCTTAGTCCCTACTA+TGG 0.419380 1_3:-66953514 Msa0135330:three_prime_UTR
GACCGTATCTGGGAGCCATT+TGG 0.421040 1_3:-66955193 Msa0135330:CDS
TTAAACCAGGATTGAGCATC+AGG 0.424108 1_3:-66953660 Msa0135330:intron
TTAAGCTGCTGATGTTCCCT+AGG 0.426912 1_3:+66953914 None:intergenic
GTGTGCCTCGGCAATACTCC+TGG 0.429095 1_3:-66954604 Msa0135330:three_prime_UTR
GCTCTCAGTTCCTTGAAAGT+TGG 0.429943 1_3:-66953294 Msa0135330:three_prime_UTR
CCATCTGCTAGTTCATCCTT+GGG 0.430876 1_3:-66954852 Msa0135330:three_prime_UTR
TTGTTACCGTTGGATGGGTT+GGG 0.434684 1_3:+66956653 None:intergenic
TTTGTTACCGTTGGATGGGT+TGG 0.440462 1_3:+66956652 None:intergenic
ATCGTTAGTGAACTGAGGTT+AGG 0.440726 1_3:+66954246 None:intergenic
GTTGAATTGCTCTGTCCAAA+TGG 0.441636 1_3:+66955178 None:intergenic
GAGCTTACAAAGTCACTATA+CGG 0.441840 1_3:+66954549 None:intergenic
ACTTCGCCAGCATTAATTAA+GGG 0.441843 1_3:+66954828 None:intergenic
ACAGAACATTCCAACTTTCA+AGG 0.443683 1_3:+66953284 None:intergenic
ATACGGTCAAACTGATCATC+AGG 0.445542 1_3:+66955208 None:intergenic
GTTAGTGAGAGCTGTCATAT+TGG 0.445640 1_3:+66954388 None:intergenic
ATACGTACTACTTATTTCTA+CGG 0.446873 1_3:-66956115 Msa0135330:CDS
TTGGAGAAGGAGTGGGAGGG+AGG 0.448824 1_3:+66956502 None:intergenic
TGGGAGGGAGGGAAGAAGGA+AGG 0.450938 1_3:+66956514 None:intergenic
GGGAAGAAGGAAGGAAAGAA+AGG 0.451508 1_3:+66956523 None:intergenic
TTGAATCGAAGTTTGATCTT+CGG 0.451829 1_3:+66953403 None:intergenic
AACTAACCTTATGCTTGGTC+CGG 0.453466 1_3:+66955793 None:intergenic
GTTACTCCGGACCAAGCATA+AGG 0.455480 1_3:-66955799 Msa0135330:intron
AAGCAAATCCCTCAAGGTGC+TGG 0.457884 1_3:+66953528 None:intergenic
AGAGCATGTGATACCGGTCC+AGG 0.459805 1_3:+66954586 None:intergenic
GCGATGTAGTATTTGGAGCT+TGG 0.467113 1_3:+66955028 None:intergenic
GACAGTTATTGCTTTGCAAA+GGG 0.467127 1_3:-66954675 Msa0135330:intron
GTTGGATGGGTTGGGTTGAA+GGG 0.468720 1_3:+66956661 None:intergenic
GGAAAGAGATGAAGAGGAGT+AGG 0.471121 1_3:+66956377 None:intergenic
GTTGATTTGGGTTGTTGTCA+GGG 0.471260 1_3:+66956300 None:intergenic
TTGGTTGCTAGAAACAGCTT+TGG 0.471469 1_3:-66955821 Msa0135330:CDS
GGCTGAGATGAAGTTTGAAT+CGG 0.472447 1_3:+66956261 None:intergenic
GGGTGTGGAAAGAGATGAAG+AGG 0.475556 1_3:+66956371 None:intergenic
TGAAGAGGAGTAGGAAGAAG+AGG 0.478178 1_3:+66956386 None:intergenic
TACTTTGCTGACAACACTGC+TGG 0.479658 1_3:-66955001 Msa0135330:CDS
CTTGGGAATAACAGTTTGTC+CGG 0.480070 1_3:-66954276 Msa0135330:three_prime_UTR
GTCCAAATGGCTCCCAGATA+CGG 0.480571 1_3:+66955191 None:intergenic
CCCAAATCAACAACCGTACA+TGG 0.482133 1_3:-66956288 Msa0135330:CDS
CAAAGTCACTATACGGATAC+GGG 0.484601 1_3:+66954556 None:intergenic
TCGTTAGTGAACTGAGGTTA+GGG 0.485132 1_3:+66954247 None:intergenic
TTGTTGTCTTTGCCAACCAA+TGG 0.486329 1_3:-66954907 Msa0135330:CDS
AACTCCAAAGCAGAAACAAA+CGG 0.491984 1_3:+66955902 None:intergenic
TCCTGGTTTAATGAGATTTG+CGG 0.493884 1_3:+66953672 None:intergenic
AGATCGTTGATGGAACGTTA+TGG 0.494471 1_3:-66956048 Msa0135330:CDS
ATTTACCTGATGCTCAATCC+TGG 0.498949 1_3:+66953655 None:intergenic
GAGATTTGCGGGAATTTGCC+CGG 0.499419 1_3:+66953684 None:intergenic
GAGGAAAGAGAATTTGGAGA+AGG 0.503398 1_3:+66956489 None:intergenic
TTGGGCCCCTTAATTAATGC+TGG 0.503842 1_3:-66954834 Msa0135330:three_prime_UTR
AGAGAATTTGGAGAAGGAGT+GGG 0.505135 1_3:+66956495 None:intergenic
TTGTTACCTCCAGCACCTTG+AGG 0.505839 1_3:-66953537 Msa0135330:three_prime_UTR
TTTAATGTGTTGTCTCTTGG+AGG 0.505920 1_3:-66954804 Msa0135330:three_prime_UTR
AAGAGAATTTGGAGAAGGAG+TGG 0.506790 1_3:+66956494 None:intergenic
CCCTTCAGAGCATGTGATAC+CGG 0.507855 1_3:+66954580 None:intergenic
AGAGGAGTAGGAAGAAGAGG+AGG 0.509998 1_3:+66956389 None:intergenic
GTTGGGTTGAAGGGTGATGA+AGG 0.510395 1_3:+66956670 None:intergenic
ACTCCAAAGCAGAAACAAAC+GGG 0.511790 1_3:+66955903 None:intergenic
GGAGAGATTCCCTCGTCCCT+AGG 0.513411 1_3:-66953931 Msa0135330:three_prime_UTR
TGACAGTTATTGCTTTGCAA+AGG 0.514133 1_3:-66954676 Msa0135330:intron
ACAAAGTCACTATACGGATA+CGG 0.514767 1_3:+66954555 None:intergenic
GATTCAAACTTCATCTCAGC+CGG 0.515966 1_3:-66956259 Msa0135330:CDS
ACCGGTATCACATGCTCTGA+AGG 0.516298 1_3:-66954581 Msa0135330:three_prime_UTR
GCTGTCATATTGGCAACAAA+AGG 0.517691 1_3:+66954398 None:intergenic
CCCGCAAATCTCATTAAACC+AGG 0.518461 1_3:-66953673 Msa0135330:three_prime_UTR
GAATTTGGAGAAGGAGTGGG+AGG 0.519945 1_3:+66956498 None:intergenic
TAGGAAGAAGAGGAGGGACA+TGG 0.526075 1_3:+66956396 None:intergenic
GCTGAGATGAAGTTTGAATC+GGG 0.537488 1_3:+66956262 None:intergenic
TGTTCAACTAACCTTATGCT+TGG 0.540415 1_3:+66955788 None:intergenic
AAGTATTGTATTAGGTGTTG+AGG 0.541091 1_3:+66956470 None:intergenic
CCTCAGTTCACTAACGATGT+TGG 0.544378 1_3:-66954241 Msa0135330:three_prime_UTR
GCTGATGTTCCCTAGGGACG+AGG 0.547688 1_3:+66953921 None:intergenic
GAGGAGTAGGAAGAAGAGGA+GGG 0.551387 1_3:+66956390 None:intergenic
CCTCGGCAATACTCCTGGAC+CGG 0.552760 1_3:-66954599 Msa0135330:three_prime_UTR
GAAACTCGAGTTATTATGAA+GGG 0.552914 1_3:-66955991 Msa0135330:CDS
GGAAACTCGAGTTATTATGA+AGG 0.553408 1_3:-66955992 Msa0135330:CDS
TAAGCTGCTGATGTTCCCTA+GGG 0.554629 1_3:+66953915 None:intergenic
TAGGACCAGAAAGAGGCCAT+TGG 0.554767 1_3:+66954891 None:intergenic
AATTATATTAATGATGACGC+GGG 0.557016 1_3:+66956553 None:intergenic
AGAAGCAAAGACAGGTTCAA+AGG 0.558486 1_3:+66956594 None:intergenic
GAGAGATTCCCTCGTCCCTA+GGG 0.563242 1_3:-66953930 Msa0135330:three_prime_UTR
GAGTATTGCCGAGGCACACA+AGG 0.563611 1_3:+66954608 None:intergenic
AGCAAAGTTGTTAAAATCGG+TGG 0.563851 1_3:+66955850 None:intergenic
TGACTTGGAGAGGGAATGAA+CGG 0.567565 1_3:+66956185 None:intergenic
TCTTTATCTCTACCATAGTA+GGG 0.568290 1_3:+66953502 None:intergenic
TCAGGGTGCCGCAGTTGATG+AGG 0.572753 1_3:+66956317 None:intergenic
CTTCGCCAGCATTAATTAAG+GGG 0.573030 1_3:+66954829 None:intergenic
TACAATATTCCTGTGTATCG+CGG 0.573154 1_3:-66956151 Msa0135330:CDS
TTGAATCGGGAAGCCATGTA+CGG 0.574693 1_3:+66956275 None:intergenic
GGAGTGTACTTTCATAATGG+TGG 0.577090 1_3:-66953337 Msa0135330:three_prime_UTR
TGATCAGTTTGACCGTATCT+GGG 0.577279 1_3:-66955203 Msa0135330:CDS
AGTTGATGAGGAAGCCTTTG+GGG 0.581668 1_3:+66956329 None:intergenic
TAGAATCCTTGGAACTGAGA+TGG 0.581775 1_3:-66955066 Msa0135330:CDS
GTGGTGATCCTTGTGTGCCT+CGG 0.581945 1_3:-66954616 Msa0135330:three_prime_UTR
CCCAAGGATGAACTAGCAGA+TGG 0.582356 1_3:+66954852 None:intergenic
AACTGGTCAGAAGCAAAGAC+AGG 0.582809 1_3:+66956586 None:intergenic
TACAAAACAACAATTTAACC+GGG 0.583593 1_3:-66953702 Msa0135330:three_prime_UTR
AAGCAATTGCATTCAAATCA+CGG 0.585518 1_3:+66954936 None:intergenic
AATTTGGAGAAGGAGTGGGA+GGG 0.586295 1_3:+66956499 None:intergenic
TATTTCTACGGTGGAGTCAA+CGG 0.588720 1_3:-66956103 Msa0135330:CDS
AGAAACAGCTTTGGTTACTC+CGG 0.591518 1_3:-66955812 Msa0135330:CDS
ACTGAGGTTAGGGATCTGTC+CGG 0.598198 1_3:+66954257 None:intergenic
GTTGATGAGGAAGCCTTTGG+GGG 0.601306 1_3:+66956330 None:intergenic
TTGATGAGGAAGCCTTTGGG+GGG 0.604688 1_3:+66956331 None:intergenic
ACCAGAAAGAGGCCATTGGT+TGG 0.608323 1_3:+66954895 None:intergenic
GGATCACCACTCCAATCAAG+TGG 0.612058 1_3:+66954629 None:intergenic
TGTTGATCATCCTAAGCCGC+CGG 0.612813 1_3:-66956080 Msa0135330:CDS
CCGGTATCACATGCTCTGAA+GGG 0.614201 1_3:-66954580 Msa0135330:three_prime_UTR
ATAAGCCATACCGTCTCGAG+TGG 0.617816 1_3:+66954773 None:intergenic
AGCATTAATTAAGGGGCCCA+AGG 0.619899 1_3:+66954836 None:intergenic
GTATGCTCTACAGCGAGAGA+AGG 0.623123 1_3:+66953380 None:intergenic
GGTTGAAGGGTGATGAAGGA+CGG 0.625871 1_3:+66956674 None:intergenic
GGGACTAAGCAAATCCCTCA+AGG 0.626657 1_3:+66953522 None:intergenic
GGTAGGCCATCTCAGTTCCA+AGG 0.630998 1_3:+66955060 None:intergenic
TAATTATATTAATGATGACG+CGG 0.631337 1_3:+66956552 None:intergenic
TTTGGAGCTTGGAAGTGAAG+CGG 0.632649 1_3:+66955039 None:intergenic
TTGGAAGAGAATCAATTCAG+TGG 0.634172 1_3:-66953952 Msa0135330:three_prime_UTR
GAGCTTGGAAGTGAAGCGGT+AGG 0.637426 1_3:+66955043 None:intergenic
CAAATCCCTCAAGGTGCTGG+AGG 0.638003 1_3:+66953531 None:intergenic
GGAAGAACTTCCACTCGAGA+CGG 0.641422 1_3:-66954783 Msa0135330:intron
CTTCAACCCAACCCATCCAA+CGG 0.644472 1_3:-66956659 Msa0135330:five_prime_UTR
CGTACTACTTATTTCTACGG+TGG 0.644648 1_3:-66956112 Msa0135330:CDS
CTGATGTTCCCTAGGGACGA+GGG 0.647895 1_3:+66953922 None:intergenic
AATCCTCCACTTGATTGGAG+TGG 0.649294 1_3:-66954635 Msa0135330:three_prime_UTR
TGATGGAACGTTATGGAGTG+TGG 0.649517 1_3:-66956041 Msa0135330:CDS
AAACTCGAGTTATTATGAAG+GGG 0.650026 1_3:-66955990 Msa0135330:CDS
AATCAAGTGGAGGATTTCGG+AGG 0.651188 1_3:+66954642 None:intergenic
TCACCACTCCAATCAAGTGG+AGG 0.674124 1_3:+66954632 None:intergenic
CCGGTCCAGGAGTATTGCCG+AGG 0.681333 1_3:+66954599 None:intergenic
ATGGAGTGTGGTGAATACTA+CGG 0.682749 1_3:-66956029 Msa0135330:CDS
AGTTGGAAGAAGGACTTGCG+TGG 0.687774 1_3:+66956219 None:intergenic
TCAACGATCTGATCAAACAC+CGG 0.691190 1_3:+66956061 None:intergenic
CCAACATCGTTAGTGAACTG+AGG 0.694401 1_3:+66954241 None:intergenic
ACGATCTGATCAAACACCGG+CGG 0.737101 1_3:+66956064 None:intergenic
AAAGTCACTATACGGATACG+GGG 0.739663 1_3:+66954557 None:intergenic
AAAGGCTTCCTCATCAACTG+CGG 0.756134 1_3:-66956325 Msa0135330:CDS
GAGTGTGGTGAATACTACGG+TGG 0.768044 1_3:-66956026 Msa0135330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATGAAAAAGTATTGTATT+AGG + chr1_3:66953483-66953502 None:intergenic 15.0%
!! AATGTTTATTATAGTTGTAT+TGG - chr1_3:66954610-66954629 Msa0135330:three_prime_UTR 15.0%
!!! ATTTTTAACATAAGTGTAAA+TGG - chr1_3:66954971-66954990 Msa0135330:CDS 15.0%
!!! AATATTGTTATTGTGTTTTA+AGG - chr1_3:66955964-66955983 Msa0135330:CDS 15.0%
!!! TAATTATATTAATGATGACG+CGG + chr1_3:66953393-66953412 None:intergenic 20.0%
!!! AAGTTTATGAGTTTTTGTAT+CGG - chr1_3:66953992-66954011 Msa0135330:intron 20.0%
!! AATAGCAAAGTTGTTAAAAT+CGG + chr1_3:66954098-66954117 None:intergenic 20.0%
!!! ACAAAAAGAATAAAAGCATA+GGG + chr1_3:66954288-66954307 None:intergenic 20.0%
!!! ATCTTCAAGTATTTTATATG+CGG + chr1_3:66954545-66954564 None:intergenic 20.0%
!! ACTATAAAAATTGAACAAGA+AGG + chr1_3:66954673-66954692 None:intergenic 20.0%
!!! TTTATAGTCATCTCTAATTT+TGG - chr1_3:66954685-66954704 Msa0135330:intron 20.0%
!!! TTATAGTCATCTCTAATTTT+GGG - chr1_3:66954686-66954705 Msa0135330:intron 20.0%
!!! AAAGTATTTGAAACACATTT+AGG - chr1_3:66954842-66954861 Msa0135330:three_prime_UTR 20.0%
!!! TTAAATTTTTTTGATCCGTA+AGG - chr1_3:66955623-66955642 Msa0135330:intron 20.0%
!! TGAATGAAAAAATTGTAGAT+TGG + chr1_3:66955817-66955836 None:intergenic 20.0%
!! AGATAACTTCAATATATTCA+TGG - chr1_3:66955928-66955947 Msa0135330:CDS 20.0%
!! TTACCATACATGTTTAAATT+AGG - chr1_3:66956137-66956156 Msa0135330:CDS 20.0%
!! TACCATACATGTTTAAATTA+GGG - chr1_3:66956138-66956157 Msa0135330:CDS 20.0%
!! TTACAAAACAACAATTTAAC+CGG - chr1_3:66956239-66956258 Msa0135330:CDS 20.0%
!! AATTATATTAATGATGACGC+GGG + chr1_3:66953392-66953411 None:intergenic 25.0%
!!! ATACGTACTACTTATTTCTA+CGG - chr1_3:66953827-66953846 Msa0135330:intron 25.0%
!! AGTTTATGAGTTTTTGTATC+GGG - chr1_3:66953993-66954012 Msa0135330:intron 25.0%
!!! GATTTTAACAACTTTGCTAT+TGG - chr1_3:66954097-66954116 Msa0135330:intron 25.0%
! TTCTGTAGATCATTATCATT+TGG - chr1_3:66954219-66954238 Msa0135330:intron 25.0%
! AGATCATTATCATTTGGTTA+TGG - chr1_3:66954225-66954244 Msa0135330:intron 25.0%
!! CACAAAAAGAATAAAAGCAT+AGG + chr1_3:66954289-66954308 None:intergenic 25.0%
! AATTCTCCTCAAGTAATTAA+GGG - chr1_3:66954320-66954339 Msa0135330:intron 25.0%
!! TGTGTTTCAAATACTTTTGA+AGG + chr1_3:66954839-66954858 None:intergenic 25.0%
!!! TGGAGTTAAAGTTATTTTGT+AGG + chr1_3:66955073-66955092 None:intergenic 25.0%
! TTTCAGAAATTTGACAAGTA+TGG - chr1_3:66955501-66955520 Msa0135330:intron 25.0%
! TACAAAACAACAATTTAACC+GGG - chr1_3:66956240-66956259 Msa0135330:CDS 25.0%
! ATATTTATCAACAGAACTTC+CGG - chr1_3:66956372-66956391 Msa0135330:CDS 25.0%
! AAGTATTGTATTAGGTGTTG+AGG + chr1_3:66953475-66953494 None:intergenic 30.0%
! AGGGACATGGTTAAAATTTT+TGG + chr1_3:66953536-66953555 None:intergenic 30.0%
GAAACTCGAGTTATTATGAA+GGG - chr1_3:66953951-66953970 Msa0135330:three_prime_UTR 30.0%
AAACTCGAGTTATTATGAAG+GGG - chr1_3:66953952-66953971 Msa0135330:three_prime_UTR 30.0%
! TTATTATGAAGGGGTTTTCT+TGG - chr1_3:66953961-66953980 Msa0135330:intron 30.0%
!!! CTTTGGAGTTTTTGATTCTT+GGG - chr1_3:66954053-66954072 Msa0135330:intron 30.0%
!! TTTGGAGTTTTTGATTCTTG+GGG - chr1_3:66954054-66954073 Msa0135330:intron 30.0%
!! TAACAACTTTGCTATTGGTT+TGG - chr1_3:66954102-66954121 Msa0135330:intron 30.0%
ATTACTTGAGGAGAATTCTA+TGG + chr1_3:66954317-66954336 None:intergenic 30.0%
GAATTCTCCTCAAGTAATTA+AGG - chr1_3:66954319-66954338 Msa0135330:intron 30.0%
GTTTATCCCTTAATTACTTG+AGG + chr1_3:66954329-66954348 None:intergenic 30.0%
! AAACTCTTTTCATGTGACTA+AGG - chr1_3:66954345-66954364 Msa0135330:intron 30.0%
! AACTCTTTTCATGTGACTAA+GGG - chr1_3:66954346-66954365 Msa0135330:intron 30.0%
AATAAGATGGAAACAGTCTA+TGG + chr1_3:66954461-66954480 None:intergenic 30.0%
GAGAGCTTATAGAAATAAGA+TGG + chr1_3:66954474-66954493 None:intergenic 30.0%
TCTTTCTCATGTAAGCTAAT+TGG + chr1_3:66954496-66954515 None:intergenic 30.0%
!! GTTTCAAATACTTTTGAAGG+TGG + chr1_3:66954836-66954855 None:intergenic 30.0%
ATCTGATCAATTAGAATCCT+TGG - chr1_3:66954865-66954884 Msa0135330:exon 30.0%
AAGCAATTGCATTCAAATCA+CGG + chr1_3:66955009-66955028 None:intergenic 30.0%
CAGCGCATATAAAATTGAAA+GGG + chr1_3:66955446-66955465 None:intergenic 30.0%
ACAGCGCATATAAAATTGAA+AGG + chr1_3:66955447-66955466 None:intergenic 30.0%
! GTCAAATTTCTGAAAACAAC+AGG + chr1_3:66955496-66955515 None:intergenic 30.0%
GTTCTCAGGTTTAAACATAT+CGG - chr1_3:66955583-66955602 Msa0135330:intron 30.0%
GACATAAAGAACTGTTAACT+AGG + chr1_3:66955854-66955873 None:intergenic 30.0%
TCTATAAAACACTACTGTAG+AGG + chr1_3:66955886-66955905 None:intergenic 30.0%
!!! TTATTGTGTTTTAAGGCACT+TGG - chr1_3:66955971-66955990 Msa0135330:CDS 30.0%
! AAAAGAAGTGTAAGTGATTG+TGG - chr1_3:66956049-66956068 Msa0135330:CDS 30.0%
CTCCCTAATTTAAACATGTA+TGG + chr1_3:66956143-66956162 None:intergenic 30.0%
AATCTGCTTACAACAAAACA+CGG + chr1_3:66956218-66956237 None:intergenic 30.0%
AACAATTGTAGAACTCATGA+TGG + chr1_3:66956333-66956352 None:intergenic 30.0%
ATCATGAGTTCTACAATTGT+TGG - chr1_3:66956332-66956351 Msa0135330:CDS 30.0%
AGGTAACAAGAAATTGTTTC+CGG + chr1_3:66956394-66956413 None:intergenic 30.0%
TCTTTATCTCTACCATAGTA+GGG + chr1_3:66956443-66956462 None:intergenic 30.0%
! TGCTTAGATTGTTCTGATTT+TGG - chr1_3:66956488-66956507 Msa0135330:five_prime_UTR 30.0%
! TTAGATTGTTCTGATTTTGG+TGG - chr1_3:66956491-66956510 Msa0135330:five_prime_UTR 30.0%
TTGAATCGAAGTTTGATCTT+CGG + chr1_3:66956542-66956561 None:intergenic 30.0%
!!! CAGCTTTATTGTTTTTGACT+TGG - chr1_3:66956584-66956603 Msa0135330:five_prime_UTR 30.0%
! TGATTTTGTTACCGTTGGAT+GGG + chr1_3:66953297-66953316 None:intergenic 35.0%
! TTGATTTTGTTACCGTTGGA+TGG + chr1_3:66953298-66953317 None:intergenic 35.0%
!! TGCTTTGATTTTGTTACCGT+TGG + chr1_3:66953302-66953321 None:intergenic 35.0%
TTCAAAGGACAGAACAGAAT+TGG + chr1_3:66953336-66953355 None:intergenic 35.0%
! ACTTGCGTGGTTATGTTTTT+GGG + chr1_3:66953713-66953732 None:intergenic 35.0%
!!! AGTGTTTTTTGACTTGGAGA+GGG + chr1_3:66953769-66953788 None:intergenic 35.0%
!!! TGTAGCAGTGTTTTTTGACT+TGG + chr1_3:66953775-66953794 None:intergenic 35.0%
TACAATATTCCTGTGTATCG+CGG - chr1_3:66953791-66953810 Msa0135330:intron 35.0%
GGAAACTCGAGTTATTATGA+AGG - chr1_3:66953950-66953969 Msa0135330:three_prime_UTR 35.0%
TATCGGGTCGAATAGTTATA+CGG - chr1_3:66954009-66954028 Msa0135330:intron 35.0%
AACTCCAAAGCAGAAACAAA+CGG + chr1_3:66954043-66954062 None:intergenic 35.0%
!!! GCTTTGGAGTTTTTGATTCT+TGG - chr1_3:66954052-66954071 Msa0135330:intron 35.0%
AGCAAAGTTGTTAAAATCGG+TGG + chr1_3:66954095-66954114 None:intergenic 35.0%
TGTTCAACTAACCTTATGCT+TGG + chr1_3:66954157-66954176 None:intergenic 35.0%
! GCATAAGGTTAGTTGAACAT+TGG - chr1_3:66954158-66954177 Msa0135330:intron 35.0%
TAGCTTACATGAGAAAGACT+AGG - chr1_3:66954498-66954517 Msa0135330:intron 35.0%
AGCTTACATGAGAAAGACTA+GGG - chr1_3:66954499-66954518 Msa0135330:intron 35.0%
AACTTTCTCAGTGTTTGCTT+TGG + chr1_3:66954789-66954808 None:intergenic 35.0%
ACTGAGAAAGTTTCTGTTTC+TGG - chr1_3:66954797-66954816 Msa0135330:three_prime_UTR 35.0%
!!! AAGTTTCTGTTTCTGGCTTT+TGG - chr1_3:66954804-66954823 Msa0135330:three_prime_UTR 35.0%
GTATAGAGCGATGTAGTATT+TGG + chr1_3:66954924-66954943 None:intergenic 35.0%
GATTTGAATGCAATTGCTTC+AGG - chr1_3:66955010-66955029 Msa0135330:CDS 35.0%
! TATTTTGTAGGACCAGAAAG+AGG + chr1_3:66955061-66955080 None:intergenic 35.0%
ACTTCGCCAGCATTAATTAA+GGG + chr1_3:66955117-66955136 None:intergenic 35.0%
GTCTTTAATGTGTTGTCTCT+TGG - chr1_3:66955135-66955154 Msa0135330:CDS 35.0%
TTTAATGTGTTGTCTCTTGG+AGG - chr1_3:66955138-66955157 Msa0135330:CDS 35.0%
! AACATGCGAGGTTTTCATAT+TGG - chr1_3:66955229-66955248 Msa0135330:intron 35.0%
TGACAGTTATTGCTTTGCAA+AGG - chr1_3:66955266-66955285 Msa0135330:intron 35.0%
GACAGTTATTGCTTTGCAAA+GGG - chr1_3:66955267-66955286 Msa0135330:intron 35.0%
ACAAAGTCACTATACGGATA+CGG + chr1_3:66955390-66955409 None:intergenic 35.0%
GAGCTTACAAAGTCACTATA+CGG + chr1_3:66955396-66955415 None:intergenic 35.0%
TTGACAAGTATGGATCTTTC+AGG - chr1_3:66955511-66955530 Msa0135330:intron 35.0%
! GTGTTTCTACTCAACTATGA+TGG - chr1_3:66955749-66955768 Msa0135330:intron 35.0%
TTGGAAGAGAATCAATTCAG+TGG - chr1_3:66955990-66956009 Msa0135330:CDS 35.0%
CATAATGTGTAAGTAGCTTC+CGG + chr1_3:66956075-66956094 None:intergenic 35.0%
TCCTGGTTTAATGAGATTTG+CGG + chr1_3:66956273-66956292 None:intergenic 35.0%
GTCTTTATCTCTACCATAGT+AGG + chr1_3:66956444-66956463 None:intergenic 35.0%
CTTGGAGTGTACTTTCATAA+TGG - chr1_3:66956602-66956621 Msa0135330:five_prime_UTR 35.0%
GGAACAGATTCTTTCAACTT+TGG - chr1_3:66956626-66956645 Msa0135330:five_prime_UTR 35.0%
ACAGAACATTCCAACTTTCA+AGG + chr1_3:66956661-66956680 None:intergenic 35.0%
AGAAGCAAAGACAGGTTCAA+AGG + chr1_3:66953351-66953370 None:intergenic 40.0%
AGAGAATTTGGAGAAGGAGT+GGG + chr1_3:66953450-66953469 None:intergenic 40.0%
AAGAGAATTTGGAGAAGGAG+TGG + chr1_3:66953451-66953470 None:intergenic 40.0%
GAGGAAAGAGAATTTGGAGA+AGG + chr1_3:66953456-66953475 None:intergenic 40.0%
! GGTGTTGAGGAAAGAGAATT+TGG + chr1_3:66953462-66953481 None:intergenic 40.0%
!! GTTGATTTGGGTTGTTGTCA+GGG + chr1_3:66953645-66953664 None:intergenic 40.0%
!! TGTTGATTTGGGTTGTTGTC+AGG + chr1_3:66953646-66953665 None:intergenic 40.0%
!! CCATGTACGGTTGTTGATTT+GGG + chr1_3:66953657-66953676 None:intergenic 40.0%
GCTGAGATGAAGTTTGAATC+GGG + chr1_3:66953683-66953702 None:intergenic 40.0%
GGCTGAGATGAAGTTTGAAT+CGG + chr1_3:66953684-66953703 None:intergenic 40.0%
GATTCAAACTTCATCTCAGC+CGG - chr1_3:66953683-66953702 Msa0135330:three_prime_UTR 40.0%
! GACTTGCGTGGTTATGTTTT+TGG + chr1_3:66953714-66953733 None:intergenic 40.0%
!!! GAGTTTTGAGAGTTGGAAGA+AGG + chr1_3:66953736-66953755 None:intergenic 40.0%
!!! CAGTGTTTTTTGACTTGGAG+AGG + chr1_3:66953770-66953789 None:intergenic 40.0%
!! CGTACTACTTATTTCTACGG+TGG - chr1_3:66953830-66953849 Msa0135330:intron 40.0%
! TATTTCTACGGTGGAGTCAA+CGG - chr1_3:66953839-66953858 Msa0135330:intron 40.0%
TCAACGATCTGATCAAACAC+CGG + chr1_3:66953884-66953903 None:intergenic 40.0%
GTGTTTGATCAGATCGTTGA+TGG - chr1_3:66953884-66953903 Msa0135330:intron 40.0%
! AGATCGTTGATGGAACGTTA+TGG - chr1_3:66953894-66953913 Msa0135330:intron 40.0%
ATGGAGTGTGGTGAATACTA+CGG - chr1_3:66953913-66953932 Msa0135330:three_prime_UTR 40.0%
!! ACTACGGTGGATTATGCTAA+TGG - chr1_3:66953929-66953948 Msa0135330:three_prime_UTR 40.0%
ACTCCAAAGCAGAAACAAAC+GGG + chr1_3:66954042-66954061 None:intergenic 40.0%
TTGGTTGCTAGAAACAGCTT+TGG - chr1_3:66954121-66954140 Msa0135330:intron 40.0%
! AGAAACAGCTTTGGTTACTC+CGG - chr1_3:66954130-66954149 Msa0135330:intron 40.0%
AACTAACCTTATGCTTGGTC+CGG + chr1_3:66954152-66954171 None:intergenic 40.0%
CTTACATAAGCACGTGAGAT+TGG - chr1_3:66954401-66954420 Msa0135330:three_prime_UTR 40.0%
ATACGGTCAAACTGATCATC+AGG + chr1_3:66954737-66954756 None:intergenic 40.0%
ATGATCAGTTTGACCGTATC+TGG - chr1_3:66954738-66954757 Msa0135330:intron 40.0%
TGATCAGTTTGACCGTATCT+GGG - chr1_3:66954739-66954758 Msa0135330:intron 40.0%
GTTGAATTGCTCTGTCCAAA+TGG + chr1_3:66954767-66954786 None:intergenic 40.0%
TAGAATCCTTGGAACTGAGA+TGG - chr1_3:66954876-66954895 Msa0135330:exon 40.0%
TTGTTGTCTTTGCCAACCAA+TGG - chr1_3:66955035-66955054 Msa0135330:CDS 40.0%
CTTCGCCAGCATTAATTAAG+GGG + chr1_3:66955116-66955135 None:intergenic 40.0%
GACTTCGCCAGCATTAATTA+AGG + chr1_3:66955118-66955137 None:intergenic 40.0%
!! ATTTTAATCGCGAACATGCG+AGG - chr1_3:66955217-66955236 Msa0135330:intron 40.0%
TCCAATCAAGTGGAGGATTT+CGG + chr1_3:66955306-66955325 None:intergenic 40.0%
AAAGTCACTATACGGATACG+GGG + chr1_3:66955388-66955407 None:intergenic 40.0%
CAAAGTCACTATACGGATAC+GGG + chr1_3:66955389-66955408 None:intergenic 40.0%
GCTGTCATATTGGCAACAAA+AGG + chr1_3:66955547-66955566 None:intergenic 40.0%
GTTAGTGAGAGCTGTCATAT+TGG + chr1_3:66955557-66955576 None:intergenic 40.0%
TCACTAACATGTAGGTTCTC+AGG - chr1_3:66955569-66955588 Msa0135330:intron 40.0%
TGCAACAAGGAAACACCTTA+CGG + chr1_3:66955641-66955660 None:intergenic 40.0%
CTTGGGAATAACAGTTTGTC+CGG - chr1_3:66955666-66955685 Msa0135330:intron 40.0%
TCGTTAGTGAACTGAGGTTA+GGG + chr1_3:66955698-66955717 None:intergenic 40.0%
ATCGTTAGTGAACTGAGGTT+AGG + chr1_3:66955699-66955718 None:intergenic 40.0%
CCTGGTTTAATGAGATTTGC+GGG + chr1_3:66956272-66956291 None:intergenic 40.0%
TTAAACCAGGATTGAGCATC+AGG - chr1_3:66956282-66956301 Msa0135330:CDS 40.0%
ATTTACCTGATGCTCAATCC+TGG + chr1_3:66956290-66956309 None:intergenic 40.0%
TTGTAGAACTCATGATGGCA+AGG + chr1_3:66956328-66956347 None:intergenic 40.0%
! GATTTGCTTAGTCCCTACTA+TGG - chr1_3:66956428-66956447 Msa0135330:five_prime_UTR 40.0%
GGAGTGTACTTTCATAATGG+TGG - chr1_3:66956605-66956624 Msa0135330:five_prime_UTR 40.0%
! TTGTTACCGTTGGATGGGTT+GGG + chr1_3:66953292-66953311 None:intergenic 45.0%
TTTGTTACCGTTGGATGGGT+TGG + chr1_3:66953293-66953312 None:intergenic 45.0%
AACTGGTCAGAAGCAAAGAC+AGG + chr1_3:66953359-66953378 None:intergenic 45.0%
GGGAAGAAGGAAGGAAAGAA+AGG + chr1_3:66953422-66953441 None:intergenic 45.0%
AATTTGGAGAAGGAGTGGGA+GGG + chr1_3:66953446-66953465 None:intergenic 45.0%
TGAAGAGGAGTAGGAAGAAG+AGG + chr1_3:66953559-66953578 None:intergenic 45.0%
GGAAAGAGATGAAGAGGAGT+AGG + chr1_3:66953568-66953587 None:intergenic 45.0%
TTTGAGAAAAAGAGGGGGTG+TGG + chr1_3:66953589-66953608 None:intergenic 45.0%
GGGTGTTTGAGAAAAAGAGG+GGG + chr1_3:66953594-66953613 None:intergenic 45.0%
! GGGGTGTTTGAGAAAAAGAG+GGG + chr1_3:66953595-66953614 None:intergenic 45.0%
!! GGGGGTGTTTGAGAAAAAGA+GGG + chr1_3:66953596-66953615 None:intergenic 45.0%
! TTTTCTCAAACACCCCCCAA+AGG - chr1_3:66953599-66953618 Msa0135330:intron 45.0%
!! AGTTGATGAGGAAGCCTTTG+GGG + chr1_3:66953616-66953635 None:intergenic 45.0%
!! CAGTTGATGAGGAAGCCTTT+GGG + chr1_3:66953617-66953636 None:intergenic 45.0%
AAAGGCTTCCTCATCAACTG+CGG - chr1_3:66953617-66953636 Msa0135330:intron 45.0%
CCCAAATCAACAACCGTACA+TGG - chr1_3:66953654-66953673 Msa0135330:intron 45.0%
! GCCATGTACGGTTGTTGATT+TGG + chr1_3:66953658-66953677 None:intergenic 45.0%
TTGAATCGGGAAGCCATGTA+CGG + chr1_3:66953670-66953689 None:intergenic 45.0%
!!! GGTTATGTTTTTGGGTGTGC+CGG + chr1_3:66953705-66953724 None:intergenic 45.0%
!!! GAACGGAGAGTTTTGAGAGT+TGG + chr1_3:66953743-66953762 None:intergenic 45.0%
! TGACTTGGAGAGGGAATGAA+CGG + chr1_3:66953760-66953779 None:intergenic 45.0%
! TATTTTGCACCGCGATACAC+AGG + chr1_3:66953803-66953822 None:intergenic 45.0%
TGATGGAACGTTATGGAGTG+TGG - chr1_3:66953901-66953920 Msa0135330:intron 45.0%
!! TGACCCGTTTGTTTCTGCTT+TGG - chr1_3:66954036-66954055 Msa0135330:intron 45.0%
GCATAGGGCAACGAAAACTT+AGG + chr1_3:66954273-66954292 None:intergenic 45.0%
CATGTGACTAAGGGTCTGTT+TGG - chr1_3:66954355-66954374 Msa0135330:intron 45.0%
GACTAAGGGTCTGTTTGGAT+TGG - chr1_3:66954360-66954379 Msa0135330:intron 45.0%
CATAAGCACGTGAGATTGGT+CGG - chr1_3:66954405-66954424 Msa0135330:three_prime_UTR 45.0%
! TTTGGAGCTTGGAAGTGAAG+CGG + chr1_3:66954906-66954925 None:intergenic 45.0%
! GCGATGTAGTATTTGGAGCT+TGG + chr1_3:66954917-66954936 None:intergenic 45.0%
TACTTTGCTGACAACACTGC+TGG - chr1_3:66954941-66954960 Msa0135330:CDS 45.0%
TCCATCTGCTAGTTCATCCT+TGG - chr1_3:66955089-66955108 Msa0135330:CDS 45.0%
CCATCTGCTAGTTCATCCTT+GGG - chr1_3:66955090-66955109 Msa0135330:CDS 45.0%
AGCATTAATTAAGGGGCCCA+AGG + chr1_3:66955109-66955128 None:intergenic 45.0%
TTGGGCCCCTTAATTAATGC+TGG - chr1_3:66955108-66955127 Msa0135330:CDS 45.0%
AATCAAGTGGAGGATTTCGG+AGG + chr1_3:66955303-66955322 None:intergenic 45.0%
TCCGAAATCCTCCACTTGAT+TGG - chr1_3:66955302-66955321 Msa0135330:intron 45.0%
AATCCTCCACTTGATTGGAG+TGG - chr1_3:66955307-66955326 Msa0135330:intron 45.0%
GACAGCTCTCACTAACATGT+AGG - chr1_3:66955561-66955580 Msa0135330:intron 45.0%
CCAACATCGTTAGTGAACTG+AGG + chr1_3:66955704-66955723 None:intergenic 45.0%
CCTCAGTTCACTAACGATGT+TGG - chr1_3:66955701-66955720 Msa0135330:intron 45.0%
TAAGCTGCTGATGTTCCCTA+GGG + chr1_3:66956030-66956049 None:intergenic 45.0%
TTAAGCTGCTGATGTTCCCT+AGG + chr1_3:66956031-66956050 None:intergenic 45.0%
GAAGTGTAAGTGATTGTGGC+CGG - chr1_3:66956053-66956072 Msa0135330:CDS 45.0%
CCCGCAAATCTCATTAAACC+AGG - chr1_3:66956269-66956288 Msa0135330:CDS 45.0%
CTCATGATGGCAAGGTGAAA+AGG + chr1_3:66956320-66956339 None:intergenic 45.0%
GCTCTCAGTTCCTTGAAAGT+TGG - chr1_3:66956648-66956667 Msa0135330:five_prime_UTR 45.0%
GGTTGAAGGGTGATGAAGGA+CGG + chr1_3:66953271-66953290 None:intergenic 50.0%
!! GTTGGGTTGAAGGGTGATGA+AGG + chr1_3:66953275-66953294 None:intergenic 50.0%
!! GTTGGATGGGTTGGGTTGAA+GGG + chr1_3:66953284-66953303 None:intergenic 50.0%
CTTCAACCCAACCCATCCAA+CGG - chr1_3:66953283-66953302 Msa0135330:three_prime_UTR 50.0%
ACGCGGGAAACTGTGTTAAC+TGG + chr1_3:66953376-66953395 None:intergenic 50.0%
GAATTTGGAGAAGGAGTGGG+AGG + chr1_3:66953447-66953466 None:intergenic 50.0%
TAGGAAGAAGAGGAGGGACA+TGG + chr1_3:66953549-66953568 None:intergenic 50.0%
GAGGAGTAGGAAGAAGAGGA+GGG + chr1_3:66953555-66953574 None:intergenic 50.0%
AGAGGAGTAGGAAGAAGAGG+AGG + chr1_3:66953556-66953575 None:intergenic 50.0%
GGGTGTGGAAAGAGATGAAG+AGG + chr1_3:66953574-66953593 None:intergenic 50.0%
!! GGGGGGTGTTTGAGAAAAAG+AGG + chr1_3:66953597-66953616 None:intergenic 50.0%
TTGATGAGGAAGCCTTTGGG+GGG + chr1_3:66953614-66953633 None:intergenic 50.0%
! GTTGATGAGGAAGCCTTTGG+GGG + chr1_3:66953615-66953634 None:intergenic 50.0%
!! GCAGTTGATGAGGAAGCCTT+TGG + chr1_3:66953618-66953637 None:intergenic 50.0%
AGTTGGAAGAAGGACTTGCG+TGG + chr1_3:66953726-66953745 None:intergenic 50.0%
TGTTGATCATCCTAAGCCGC+CGG - chr1_3:66953862-66953881 Msa0135330:intron 50.0%
ACGATCTGATCAAACACCGG+CGG + chr1_3:66953881-66953900 None:intergenic 50.0%
GAGTGTGGTGAATACTACGG+TGG - chr1_3:66953916-66953935 Msa0135330:three_prime_UTR 50.0%
! TGAAGGGGTTTTCTTGGCTG+TGG - chr1_3:66953967-66953986 Msa0135330:intron 50.0%
! GAAGGGGTTTTCTTGGCTGT+GGG - chr1_3:66953968-66953987 Msa0135330:intron 50.0%
! AAGGGGTTTTCTTGGCTGTG+GGG - chr1_3:66953969-66953988 Msa0135330:intron 50.0%
GTTACTCCGGACCAAGCATA+AGG - chr1_3:66954143-66954162 Msa0135330:intron 50.0%
CTAGGGAATGAGCACGTAGT+AGG - chr1_3:66954516-66954535 Msa0135330:intron 50.0%
GTCCAAATGGCTCCCAGATA+CGG + chr1_3:66954754-66954773 None:intergenic 50.0%
ACCAGAAAGAGGCCATTGGT+TGG + chr1_3:66955050-66955069 None:intergenic 50.0%
TAGGACCAGAAAGAGGCCAT+TGG + chr1_3:66955054-66955073 None:intergenic 50.0%
CCCAAGGATGAACTAGCAGA+TGG + chr1_3:66955093-66955112 None:intergenic 50.0%
GGAAGAACTTCCACTCGAGA+CGG - chr1_3:66955159-66955178 Msa0135330:CDS 50.0%
AACTTCCACTCGAGACGGTA+TGG - chr1_3:66955164-66955183 Msa0135330:CDS 50.0%
ATAAGCCATACCGTCTCGAG+TGG + chr1_3:66955172-66955191 None:intergenic 50.0%
TCACCACTCCAATCAAGTGG+AGG + chr1_3:66955313-66955332 None:intergenic 50.0%
GGATCACCACTCCAATCAAG+TGG + chr1_3:66955316-66955335 None:intergenic 50.0%
ACCGGTATCACATGCTCTGA+AGG - chr1_3:66955361-66955380 Msa0135330:intron 50.0%
CCCTTCAGAGCATGTGATAC+CGG + chr1_3:66955365-66955384 None:intergenic 50.0%
! CCGGTATCACATGCTCTGAA+GGG - chr1_3:66955362-66955381 Msa0135330:intron 50.0%
AGGTGTTTCCTTGTTGCAGC+TGG - chr1_3:66955643-66955662 Msa0135330:intron 50.0%
TTTCCTTGTTGCAGCTGGCT+TGG - chr1_3:66955648-66955667 Msa0135330:intron 50.0%
! TTCCTTGTTGCAGCTGGCTT+GGG - chr1_3:66955649-66955668 Msa0135330:intron 50.0%
ACTGAGGTTAGGGATCTGTC+CGG + chr1_3:66955688-66955707 None:intergenic 50.0%
GAGATTTGCGGGAATTTGCC+CGG + chr1_3:66956261-66956280 None:intergenic 50.0%
TTGTTACCTCCAGCACCTTG+AGG - chr1_3:66956405-66956424 Msa0135330:exon 50.0%
TGTTACCTCCAGCACCTTGA+GGG - chr1_3:66956406-66956425 Msa0135330:exon 50.0%
AAGCAAATCCCTCAAGGTGC+TGG + chr1_3:66956417-66956436 None:intergenic 50.0%
GGGACTAAGCAAATCCCTCA+AGG + chr1_3:66956423-66956442 None:intergenic 50.0%
GTATGCTCTACAGCGAGAGA+AGG + chr1_3:66956565-66956584 None:intergenic 50.0%
!! CGTTGGATGGGTTGGGTTGA+AGG + chr1_3:66953285-66953304 None:intergenic 55.0%
TGATCAAACACCGGCGGCTT+AGG + chr1_3:66953875-66953894 None:intergenic 55.0%
! CACGTGAGATTGGTCGGAAG+AGG - chr1_3:66954411-66954430 Msa0135330:three_prime_UTR 55.0%
GACCGTATCTGGGAGCCATT+TGG - chr1_3:66954749-66954768 Msa0135330:intron 55.0%
GGTAGGCCATCTCAGTTCCA+AGG + chr1_3:66954885-66954904 None:intergenic 55.0%
GAGCTTGGAAGTGAAGCGGT+AGG + chr1_3:66954902-66954921 None:intergenic 55.0%
GCCAACCAATGGCCTCTTTC+TGG - chr1_3:66955046-66955065 Msa0135330:CDS 55.0%
GTGGTGATCCTTGTGTGCCT+CGG - chr1_3:66955326-66955345 Msa0135330:intron 55.0%
! GAGTATTGCCGAGGCACACA+AGG + chr1_3:66955337-66955356 None:intergenic 55.0%
AGAGCATGTGATACCGGTCC+AGG + chr1_3:66955359-66955378 None:intergenic 55.0%
TTCCCAAGCCAGCTGCAACA+AGG + chr1_3:66955654-66955673 None:intergenic 55.0%
GAGAGATTCCCTCGTCCCTA+GGG - chr1_3:66956012-66956031 Msa0135330:CDS 55.0%
CTGATGTTCCCTAGGGACGA+GGG + chr1_3:66956023-66956042 None:intergenic 55.0%
!! CAAATCCCTCAAGGTGCTGG+AGG + chr1_3:66956414-66956433 None:intergenic 55.0%
TGGGAGGGAGGGAAGAAGGA+AGG + chr1_3:66953431-66953450 None:intergenic 60.0%
TGGAGAAGGAGTGGGAGGGA+GGG + chr1_3:66953442-66953461 None:intergenic 60.0%
TTGGAGAAGGAGTGGGAGGG+AGG + chr1_3:66953443-66953462 None:intergenic 60.0%
!! TCAGGGTGCCGCAGTTGATG+AGG + chr1_3:66953628-66953647 None:intergenic 60.0%
GTGTGCCTCGGCAATACTCC+TGG - chr1_3:66955338-66955357 Msa0135330:intron 60.0%
CCTCGGCAATACTCCTGGAC+CGG - chr1_3:66955343-66955362 Msa0135330:intron 60.0%
GGAGAGATTCCCTCGTCCCT+AGG - chr1_3:66956011-66956030 Msa0135330:CDS 60.0%
GCTGATGTTCCCTAGGGACG+AGG + chr1_3:66956024-66956043 None:intergenic 60.0%
GGAGTGGGAGGGAGGGAAGA+AGG + chr1_3:66953435-66953454 None:intergenic 65.0%
!! CCGGTCCAGGAGTATTGCCG+AGG + chr1_3:66955346-66955365 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 66953256 66956708 66953256 ID=Msa0135330;Name=Msa0135330
chr1_3 mRNA 66953256 66956708 66953256 ID=Msa0135330-mRNA-1;Parent=Msa0135330;Name=Msa0135330-mRNA-1;_AED=0.06;_eAED=0.06;_QI=292|1|1|1|0.85|0.75|8|840|322
chr1_3 exon 66953256 66953578 66953256 ID=Msa0135330-mRNA-1:exon:22937;Parent=Msa0135330-mRNA-1
chr1_3 exon 66953661 66953729 66953661 ID=Msa0135330-mRNA-1:exon:22936;Parent=Msa0135330-mRNA-1
chr1_3 exon 66953907 66953978 66953907 ID=Msa0135330-mRNA-1:exon:22935;Parent=Msa0135330-mRNA-1
chr1_3 exon 66954234 66954302 66954234 ID=Msa0135330-mRNA-1:exon:22934;Parent=Msa0135330-mRNA-1
chr1_3 exon 66954386 66954457 66954386 ID=Msa0135330-mRNA-1:exon:22933;Parent=Msa0135330-mRNA-1
chr1_3 exon 66954557 66954692 66954557 ID=Msa0135330-mRNA-1:exon:22932;Parent=Msa0135330-mRNA-1
chr1_3 exon 66954784 66955234 66954784 ID=Msa0135330-mRNA-1:exon:22931;Parent=Msa0135330-mRNA-1
chr1_3 exon 66955800 66956708 66955800 ID=Msa0135330-mRNA-1:exon:22930;Parent=Msa0135330-mRNA-1
chr1_3 five_prime_UTR 66956417 66956708 66956417 ID=Msa0135330-mRNA-1:five_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 CDS 66955800 66956416 66955800 ID=Msa0135330-mRNA-1:cds;Parent=Msa0135330-mRNA-1
chr1_3 CDS 66954883 66955234 66954883 ID=Msa0135330-mRNA-1:cds;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66954784 66954882 66954784 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66954557 66954692 66954557 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66954386 66954457 66954386 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66954234 66954302 66954234 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66953907 66953978 66953907 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66953661 66953729 66953661 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
chr1_3 three_prime_UTR 66953256 66953578 66953256 ID=Msa0135330-mRNA-1:three_prime_utr;Parent=Msa0135330-mRNA-1
Gene Sequence

>Msa0135330

ATGTCCCTCCTCTTCTTCCTACTCCTCTTCATCTCTTTCCACACCCCCTCTTTTTCTCAAACACCCCCCAAAGGCTTCCTCATCAACTGCGGCACCCTGACAACAACCCAAATCAACAACCGTACATGGCTTCCCGATTCAAACTTCATCTCAGCCGGCACACCCAAAAACATAACCACGCAAGTCCTTCTTCCAACTCTCAAAACTCTCCGTTCATTCCCTCTCCAAGTCAAAAAACACTGCTACAATATTCCTGTGTATCGCGGTGCAAAATATATGATACGTACTACTTATTTCTACGGTGGAGTCAACGGTGTTGATCATCCTAAGCCGCCGGTGTTTGATCAGATCGTTGATGGAACGTTATGGAGTGTGGTGAATACTACGGTGGATTATGCTAATGGAAACTCGAGTTATTATGAAGGGGTTTTCTTGGCTGTGGGGAAGTTTATGAGTTTTTGTATCGGGTCGAATAGTTATACGGATTCTGACCCGTTTGTTTCTGCTTTGGAGTTTTTGATTCTTGGGGATTCGTTGTATAATACCACCGATTTTAACAACTTTGCTATTGGTTTGGTTGCTAGAAACAGCTTTGGTTACTCCGGACCAAGCATAAGATTTCCTGATGATCAGTTTGACCGTATCTGGGAGCCATTTGGACAGAGCAATTCAACCAAAGCAAACACTGAGAAAGTTTCTGTTTCTGGCTTTTGGAATCTTCCACCTTCAAAAGTATTTGAAACACATTTAGGATCTGATCAATTAGAATCCTTGGAACTGAGATGGCCTACCGCTTCACTTCCAAGCTCCAAATACTACATCGCTCTATACTTTGCTGACAACACTGCTGGATCAAGAATTTTTAACATAAGTGTAAATGGCGTGCATTATTACCGTGATTTGAATGCAATTGCTTCAGGAGTTGTTGTCTTTGCCAACCAATGGCCTCTTTCTGGTCCTACAAAATAA
Protein sequence

>Msa0135330

MSLLFFLLLFISFHTPSFSQTPPKGFLINCGTLTTTQINNRTWLPDSNFISAGTPKNITTQVLLPTLKTLRSFPLQVKKHCYNIPVYRGAKYMIRTTYFYGGVNGVDHPKPPVFDQIVDGTLWSVVNTTVDYANGNSSYYEGVFLAVGKFMSFCIGSNSYTDSDPFVSALEFLILGDSLYNTTDFNNFAIGLVARNSFGYSGPSIRFPDDQFDRIWEPFGQSNSTKANTEKVSVSGFWNLPPSKVFETHLGSDQLESLELRWPTASLPSSKYYIALYFADNTAGSRIFNISVNGVHYYRDLNAIASGVVVFANQWPLSGPTK*