AlfalfaGEDB | Msa0096120

M. sativa cultivar zhongmu-4 / Msa0096120

KEGG: K12486
GO: -
PFAM: PF16720.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096120	AET02871.1	91.398	93	8	0	1	93	9	101	2.95e-55	176

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096120	sp\|P62929\|ALB1D_PEA	46.667	75	36	2	3	76	56	127	5.31e-19	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096120	G7LB06	91.398	93	8	0	1	93	9	101	1.41e-55	176

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0096120	Msa0657720	0.805639	1.211616e-49	-8.615850e-47
Msa0096120	Msa0702790	0.800905	1.157618e-48	-8.615850e-47
Msa0096120	Msa0828950	0.803834	2.884769e-49	-8.615850e-47
Msa0096120	Msa0993190	0.808858	2.518443e-50	-8.615850e-47
Msa0096120	Msa1143230	0.942487	6.589965e-102	-8.615850e-47
Msa0096120	Msa1243340	0.942884	3.251656e-102	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0096120	MtrunA17_Chr8g0357331	91.398	93	8	0	1	93	9	101	2.71e-59	176
Msa0096120	MtrunA17_Chr7g0232011	69.149	94	28	1	1	93	52	145	4.27e-44	139
Msa0096120	MtrunA17_Chr7g0231991	70.588	85	24	1	10	93	48	132	2.58e-36	119
Msa0096120	MtrunA17_Chr3g0090571	51.852	81	39	0	13	93	74	154	4.45e-27	96.7
Msa0096120	MtrunA17_Chr8g0359071	65.672	67	20	1	3	66	58	124	1.61e-26	94.7
Msa0096120	MtrunA17_Chr8g0344801	52.500	80	35	2	1	77	17	96	1.15e-23	86.7
Msa0096120	MtrunA17_Chr6g0456991	62.069	58	21	1	10	66	48	105	8.50e-23	84.7
Msa0096120	MtrunA17_Chr8g0344771	57.576	66	27	1	10	74	44	109	3.08e-22	83.6
Msa0096120	MtrunA17_Chr8g0344791	56.061	66	28	1	10	74	44	109	8.19e-22	82.4
Msa0096120	MtrunA17_Chr6g0464071	44.828	87	37	1	3	89	57	132	1.51e-21	82.0
Msa0096120	MtrunA17_Chr7g0236801	42.857	91	39	2	1	89	58	137	4.90e-21	80.9
Msa0096120	MtrunA17_Chr6g0456951	53.333	75	33	2	1	73	56	130	5.58e-21	80.9
Msa0096120	MtrunA17_Chr4g0012121	43.750	96	40	3	1	93	61	145	5.47e-20	78.6
Msa0096120	MtrunA17_Chr3g0111311	57.895	57	24	0	3	59	35	91	1.74e-19	75.5
Msa0096120	MtrunA17_Chr3g0111131	50.000	64	32	0	2	65	58	121	2.72e-19	76.3
Msa0096120	MtrunA17_Chr3g0111111	46.753	77	36	2	3	74	59	135	3.48e-19	76.3
Msa0096120	MtrunA17_Chr7g0236791	43.333	90	39	2	1	89	58	136	4.37e-18	73.2
Msa0096120	MtrunA17_Chr3g0111351	45.977	87	36	2	3	89	67	142	5.19e-18	73.2
Msa0096120	MtrunA17_Chr3g0111241	54.237	59	27	0	1	59	58	116	8.97e-18	72.4
Msa0096120	MtrunA17_Chr3g0104571	46.250	80	31	3	13	91	68	136	1.09e-17	72.4
Msa0096120	MtrunA17_Chr3g0111221	46.575	73	38	1	3	74	37	109	1.27e-17	71.6
Msa0096120	MtrunA17_Chr3g0111081	43.023	86	38	2	3	88	57	131	2.54e-17	71.2
Msa0096120	MtrunA17_Chr3g0111071	41.860	86	39	2	3	88	57	131	1.02e-16	69.7
Msa0096120	MtrunA17_Chr3g0111191	50.847	59	25	1	3	57	59	117	1.83e-16	68.6
Msa0096120	MtrunA17_Chr3g0111281	49.231	65	32	1	11	74	71	135	1.51e-14	64.3
Msa0096120	MtrunA17_Chr1g0153591	41.935	62	34	1	4	63	46	107	2.59e-14	63.2
Msa0096120	MtrunA17_Chr3g0111261	48.571	70	33	2	6	74	64	131	3.98e-13	60.5
Msa0096120	MtrunA17_Chr6g0468601	40.984	61	36	0	11	71	68	128	7.90e-13	59.7
Msa0096120	MtrunA17_Chr6g0468621	40.244	82	38	2	11	92	64	134	2.27e-12	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 24 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGTTCTGCTTCAACGTTAG+AGG	0.288036	1_3:+1601707	None:intergenic
CCATGTTGGAGCTCAGAATT+AGG	0.295933	1_3:+1601741	None:intergenic
GACTGCATCAAGAAGGGAAC+TGG	0.398345	1_3:-1601783	Msa0096120:CDS
GAATTTGTTTATTGCAAGGT+TGG	0.400644	1_3:+1601668	None:intergenic
ATAGCAGCGGCAGGATTGGC+TGG	0.400771	1_3:-1601876	None:intergenic
GTCATGAGATTGACATAAGT+TGG	0.456131	1_3:+1601806	None:intergenic
TTGAACATATCAGGATTAAG+TGG	0.466761	1_3:+1601587	None:intergenic
TGAGGAATTTGTTTATTGCA+AGG	0.514801	1_3:+1601664	None:intergenic
TCATGAGATTGACATAAGTT+GGG	0.516254	1_3:+1601807	None:intergenic
GATTAAGTGGCAATCTCCAC+AGG	0.518992	1_3:+1601600	None:intergenic
ATTCTGAGCTCCAACATGGC+TGG	0.533075	1_3:-1601737	Msa0096120:CDS
TAGCAGCGGCAGGATTGGCT+GGG	0.538187	1_3:-1601875	None:intergenic
ACCACAGTTAACTATTACGA+AGG	0.540571	1_3:-1601844	Msa0096120:CDS
TCATGACGACTGCATCAAGA+AGG	0.549615	1_3:-1601790	Msa0096120:CDS
TGAAGCAGAACAGTACTTCG+AGG	0.550769	1_3:-1601697	Msa0096120:CDS
TCGTAATAGTTAACTGTGGT+CGG	0.573176	1_3:+1601847	None:intergenic
AGGTAAAACACCAGCCATGT+TGG	0.578092	1_3:+1601727	None:intergenic
AACTATTACGAAGGCAGTCG+AGG	0.583300	1_3:-1601835	Msa0096120:CDS
CCTAATTCTGAGCTCCAACA+TGG	0.593295	1_3:-1601741	Msa0096120:CDS
TGGAGTAAAAGATATTCTTG+AGG	0.626728	1_3:+1601646	None:intergenic
CATGACGACTGCATCAAGAA+GGG	0.629712	1_3:-1601789	Msa0096120:CDS
ACTGCATCAAGAAGGGAACT+GGG	0.650923	1_3:-1601782	Msa0096120:CDS
AGGATTCTTGAAGATGCCTG+TGG	0.661804	1_3:-1601616	Msa0096120:CDS
GCCTTCGTAATAGTTAACTG+TGG	0.719007	1_3:+1601843	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAGAATATCTTTTACTCCAA+TGG	-	chr1_3:1601821-1601840	Msa0096120:CDS	25.0%
	TCATGAGATTGACATAAGTT+GGG	+	chr1_3:1601659-1601678	None:intergenic	30.0%
!	GAATTTGTTTATTGCAAGGT+TGG	+	chr1_3:1601798-1601817	None:intergenic	30.0%
!	TGAGGAATTTGTTTATTGCA+AGG	+	chr1_3:1601802-1601821	None:intergenic	30.0%
	TGGAGTAAAAGATATTCTTG+AGG	+	chr1_3:1601820-1601839	None:intergenic	30.0%
!	ATCTTTTACTCCAATGGAAA+AGG	-	chr1_3:1601827-1601846	Msa0096120:CDS	30.0%
	TCGTAATAGTTAACTGTGGT+CGG	+	chr1_3:1601619-1601638	None:intergenic	35.0%
	ACCACAGTTAACTATTACGA+AGG	-	chr1_3:1601619-1601638	Msa0096120:CDS	35.0%
	GTCATGAGATTGACATAAGT+TGG	+	chr1_3:1601660-1601679	None:intergenic	35.0%
!	CTTCAAGAATCCTTTTCCAT+TGG	+	chr1_3:1601840-1601859	None:intergenic	35.0%
	GCCTTCGTAATAGTTAACTG+TGG	+	chr1_3:1601623-1601642	None:intergenic	40.0%
	AACTATTACGAAGGCAGTCG+AGG	-	chr1_3:1601628-1601647	Msa0096120:CDS	45.0%
	TCATGACGACTGCATCAAGA+AGG	-	chr1_3:1601673-1601692	Msa0096120:CDS	45.0%
	CATGACGACTGCATCAAGAA+GGG	-	chr1_3:1601674-1601693	Msa0096120:CDS	45.0%
	ACTGCATCAAGAAGGGAACT+GGG	-	chr1_3:1601681-1601700	Msa0096120:CDS	45.0%
	CCATGTTGGAGCTCAGAATT+AGG	+	chr1_3:1601725-1601744	None:intergenic	45.0%
	CCTAATTCTGAGCTCCAACA+TGG	-	chr1_3:1601722-1601741	Msa0096120:CDS	45.0%
	AGGTAAAACACCAGCCATGT+TGG	+	chr1_3:1601739-1601758	None:intergenic	45.0%
	CTGTTCTGCTTCAACGTTAG+AGG	+	chr1_3:1601759-1601778	None:intergenic	45.0%
	TGAAGCAGAACAGTACTTCG+AGG	-	chr1_3:1601766-1601785	Msa0096120:CDS	45.0%
	AGGATTCTTGAAGATGCCTG+TGG	-	chr1_3:1601847-1601866	Msa0096120:CDS	45.0%
	GACTGCATCAAGAAGGGAAC+TGG	-	chr1_3:1601680-1601699	Msa0096120:CDS	50.0%
	ATTCTGAGCTCCAACATGGC+TGG	-	chr1_3:1601726-1601745	Msa0096120:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	1601602	1601883	1601602	ID=Msa0096120;Name=Msa0096120
chr1_3	mRNA	1601602	1601883	1601602	ID=Msa0096120-mRNA-1;Parent=Msa0096120;Name=Msa0096120-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|93
chr1_3	exon	1601602	1601883	1601602	ID=Msa0096120-mRNA-1:exon:367;Parent=Msa0096120-mRNA-1
chr1_3	CDS	1601602	1601883	1601602	ID=Msa0096120-mRNA-1:cds;Parent=Msa0096120-mRNA-1

Gene Sequence	>Msa0096120 TTGGCTGGGTTTTGCCGACCACAGTTAACTATTACGAAGGCAGTCGAGGAACATCCCAACTTATGTCAATCTCATGACGACTGCATCAAGAAGGGAACTGGGAGTTTATGTGCTTATTATCCTAATTCTGAGCTCCAACATGGCTGGTGTTTTACCTCTAACGTTGAAGCAGAACAGTACTTCGAGGTTCTCTCCAACCTTGCAATAAACAAATTCCTCAAGAATATCTTTTACTCCAATGGAAAAGGATTCTTGAAGATGCCTGTGGAGATTGCCACTTAA
Protein sequence	>Msa0096120 LAGFCRPQLTITKAVEEHPNLCQSHDDCIKKGTGSLCAYYPNSELQHGWCFTSNVEAEQYFEVLSNLAINKFLKNIFYSNGKGFLKMPVEIAT*