AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0101720


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101720 MtrunA17_Chr1g0154111 82.911 158 22 2 1 153 1 158 1.89e-88 269
Msa0101720 MtrunA17_Chr1g0154111 77.174 92 18 1 36 124 368 459 1.84e-41 145
Msa0101720 MtrunA17_Chr1g0154241 79.085 153 32 0 1 153 1 153 3.74e-87 256
Msa0101720 MtrunA17_Chr1g0154211 78.667 150 32 0 4 153 5 154 1.32e-84 253
Msa0101720 MtrunA17_Chr1g0154191 77.778 153 33 1 1 152 1 153 2.04e-82 247
Msa0101720 MtrunA17_Chr1g0154131 78.065 155 30 1 1 151 1 155 2.19e-82 250
Msa0101720 MtrunA17_Chr1g0154131 77.241 145 32 1 2 146 164 307 4.05e-77 237
Msa0101720 MtrunA17_Chr1g0154101 80.795 151 28 1 2 151 5 155 3.40e-81 256
Msa0101720 MtrunA17_Chr1g0154101 73.103 145 38 1 7 151 169 312 4.70e-69 223
Msa0101720 MtrunA17_Chr1g0154171 79.720 143 29 0 4 146 5 147 7.35e-80 240
Msa0101720 MtrunA17_Chr1g0154161 78.912 147 25 2 4 149 12 153 3.20e-78 235
Msa0101720 MtrunA17_Chr1g0154251 70.701 157 39 2 2 151 7 163 1.89e-74 231
Msa0101720 MtrunA17_Chr1g0154071 76.866 134 31 0 3 136 9 142 2.31e-69 217
Msa0101720 MtrunA17_Chr1g0154071 68.310 142 44 1 10 151 145 285 1.69e-63 202
Msa0101720 MtrunA17_Chr1g0154151 68.103 116 26 1 36 151 19 123 3.57e-49 161
Msa0101720 MtrunA17_Chr1g0154041 37.255 102 63 1 21 122 15 115 9.71e-17 76.3
Msa0101720 MtrunA17_Chr7g0233561 34.146 123 79 2 22 144 19 139 8.59e-16 72.8
Msa0101720 MtrunA17_Chr3g0131891 34.021 97 63 1 20 115 17 113 4.10e-15 71.2
Msa0101720 MtrunA17_Chr7g0233571 35.294 102 65 1 15 115 3 104 7.05e-15 69.3
Msa0101720 MtrunA17_Chr1g0162441 39.080 87 53 0 25 111 11 97 5.61e-13 65.5
Msa0101720 MtrunA17_Chr7g0233531 36.709 79 50 0 37 115 2 80 5.64e-13 64.7
Msa0101720 MtrunA17_Chr5g0419201 27.419 124 87 1 22 145 17 137 2.28e-12 62.0
Msa0101720 MtrunA17_Chr5g0429931 32.558 129 80 4 30 158 5 126 1.04e-11 60.8
Msa0101720 MtrunA17_Chr4g0033961 38.372 86 53 0 26 111 11 96 1.04e-11 61.6
Msa0101720 MtrunA17_Chr1g0162411 37.363 91 57 0 25 115 11 101 3.81e-11 60.1
Msa0101720 MtrunA17_Chr8g0390841 30.392 102 70 1 15 115 32 133 7.30e-11 59.3
Msa0101720 MtrunA17_Chr7g0233541 36.486 74 47 0 37 110 2 75 8.08e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101720 AT4G33280.2 49.333 150 72 3 8 154 11 159 2.86e-46 152
Msa0101720 AT4G33280.1 52.632 133 61 2 8 139 11 142 2.40e-45 151
Msa0101720 AT4G33280.3 49.333 150 72 3 8 154 11 159 4.01e-45 152
Msa0101720 AT4G34400.2 39.450 109 61 3 15 120 8 114 1.10e-21 90.1
Msa0101720 AT4G34400.1 39.450 109 61 3 15 120 8 114 1.10e-21 90.1
Msa0101720 AT3G06220.1 34.286 105 68 1 22 126 11 114 5.46e-16 71.6
Msa0101720 AT3G18990.2 39.785 93 55 1 19 111 5 96 8.18e-16 73.6
Msa0101720 AT3G18990.1 39.785 93 55 1 19 111 5 96 8.65e-16 73.6
Msa0101720 AT3G06160.1 33.333 105 68 2 22 126 26 128 6.83e-15 70.9
Msa0101720 AT3G06160.5 33.333 105 68 2 22 126 11 113 6.93e-15 70.9
Msa0101720 AT3G06160.4 33.333 105 68 2 22 126 11 113 6.93e-15 70.9
Msa0101720 AT3G06160.3 33.333 105 68 2 22 126 11 113 6.93e-15 70.9
Msa0101720 AT3G06160.2 33.333 105 68 2 22 126 70 172 9.62e-15 70.5
Msa0101720 AT5G60130.5 39.560 91 50 2 33 118 25 115 4.96e-14 67.8
Msa0101720 AT5G60130.3 40.449 89 48 2 35 118 1 89 7.24e-14 67.8
Msa0101720 AT5G60130.2 40.449 89 48 2 35 118 27 115 8.75e-14 67.4
Msa0101720 AT5G60130.4 40.449 89 48 2 35 118 24 112 1.16e-13 67.4
Msa0101720 AT5G60130.1 40.449 89 48 2 35 118 27 115 1.16e-13 67.4
Msa0101720 AT5G66980.1 41.111 90 48 2 33 118 21 109 2.43e-13 66.6
Msa0101720 AT3G53310.1 35.443 79 51 0 33 111 22 100 3.93e-12 62.8
Msa0101720 AT3G18960.2 35.417 96 61 1 22 117 31 125 6.25e-12 61.2
Msa0101720 AT4G01580.1 35.417 96 61 1 22 117 31 125 7.10e-12 60.8
Msa0101720 AT3G18960.1 35.417 96 61 1 22 117 31 125 9.16e-12 60.8
Msa0101720 AT5G57720.1 27.500 120 86 1 19 137 10 129 1.11e-11 61.6
Msa0101720 AT5G60140.1 31.193 109 71 2 13 118 7 114 4.22e-11 60.1
Msa0101720 AT3G18960.3 37.931 87 51 2 31 117 5 88 7.99e-11 57.8

Find 33 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTCCACTAGGACCTTTA+AGG 0.178570 1_3:+8366164 None:intergenic
TAACAAGTTGTTGTTGAAAA+TGG 0.201740 1_3:+8366550 None:intergenic
CCAGAAAATGTGACCCTTAA+AGG 0.236805 1_3:-8366178 Msa0101720:CDS
AGTGGAGTTGTGTGGGATAT+TGG 0.305311 1_3:-8366151 Msa0101720:CDS
CATGGGAGGAAGAGATTTAC+TGG 0.311171 1_3:-8366617 Msa0101720:CDS
AATGGGTGGTACTCAGAAAT+TGG 0.319733 1_3:+8366568 None:intergenic
TGAAATGGATGAACTGAAAA+TGG 0.343707 1_3:+8366592 None:intergenic
TACTCAGAAATTGGGTGAAA+TGG 0.383889 1_3:+8366577 None:intergenic
GTGGAGTTGTGTGGGATATT+GGG 0.385625 1_3:-8366150 Msa0101720:CDS
CAATGGTGAATCACTCTTTG+AGG 0.437853 1_3:-8366029 Msa0101720:CDS
AACAAGTTGTTGTTGAAAAT+GGG 0.464779 1_3:+8366551 None:intergenic
TGGGAGGTGGAAGAAGTTCA+TGG 0.473237 1_3:-8366635 Msa0101720:CDS
AACTCCACTAGGACCTTTAA+GGG 0.479312 1_3:+8366165 None:intergenic
TTCCTTGTCTTTAAGTACAA+TGG 0.496096 1_3:-8366046 Msa0101720:CDS
ATGGGTGGTACTCAGAAATT+GGG 0.499844 1_3:+8366569 None:intergenic
AGATGATACTGTTTACTTCA+CGG 0.508557 1_3:-8366116 Msa0101720:CDS
TTTCATTCTCAAATGGGAGG+TGG 0.528232 1_3:-8366648 None:intergenic
CTGTTTACTTCACGGACGGT+TGG 0.547363 1_3:-8366108 Msa0101720:CDS
ATATCCCACACAACTCCACT+AGG 0.550354 1_3:+8366154 None:intergenic
CACCATTGTACTTAAAGACA+AGG 0.554483 1_3:+8366044 None:intergenic
AAGGTCCTAGTGGAGTTGTG+TGG 0.558725 1_3:-8366159 Msa0101720:CDS
GGACAAGCTCATACTGAACA+AGG 0.580719 1_3:-8365944 Msa0101720:CDS
GGGAGGTGGAAGAAGTTCAT+GGG 0.586498 1_3:-8366634 Msa0101720:CDS
AGGTCCTAGTGGAGTTGTGT+GGG 0.591978 1_3:-8366158 Msa0101720:CDS
CTATTTCATTCTCAAATGGG+AGG 0.618562 1_3:-8366651 None:intergenic
GTGACCCTTAAAGGTCCTAG+TGG 0.630968 1_3:-8366169 Msa0101720:CDS
GATACTGTTTACTTCACGGA+CGG 0.631641 1_3:-8366112 Msa0101720:CDS
AGGTGGAAGAAGTTCATGGG+AGG 0.632051 1_3:-8366631 Msa0101720:CDS
ACAAGCTCATACTGAACAAG+GGG 0.635406 1_3:-8365942 Msa0101720:CDS
GACAAGCTCATACTGAACAA+GGG 0.653892 1_3:-8365943 Msa0101720:CDS
TTCAAAGAATTCTGTCAGAG+AGG 0.666484 1_3:-8365903 Msa0101720:CDS
AAGTTGTTGTTGAAAATGGG+TGG 0.700580 1_3:+8366554 None:intergenic
CAAGCTCATACTGAACAAGG+GGG 0.792313 1_3:-8365941 Msa0101720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTCATTTAGAAGA+TGG + chr1_3:8366012-8366031 None:intergenic 20.0%
!!! GAAATTTATATTTTAACTGC+AGG - chr1_3:8366265-8366284 Msa0101720:intron 20.0%
!!! AAATTGTCTGAAAATGTTTT+AGG + chr1_3:8366296-8366315 None:intergenic 20.0%
!!! TTTGAGGTTTTAATCTTTAA+TGG - chr1_3:8366494-8366513 Msa0101720:intron 20.0%
!!! TTTTTGTTTCATTTAGAAGA+TGG + chr1_3:8366012-8366031 None:intergenic 20.0%
!!! GAAATTTATATTTTAACTGC+AGG - chr1_3:8366265-8366284 Msa0101720:intron 20.0%
!!! AAATTGTCTGAAAATGTTTT+AGG + chr1_3:8366296-8366315 None:intergenic 20.0%
!!! TTTGAGGTTTTAATCTTTAA+TGG - chr1_3:8366494-8366513 Msa0101720:intron 20.0%
!! AACAAGTTGTTGTTGAAAAT+GGG + chr1_3:8365959-8365978 None:intergenic 25.0%
!! TAACAAGTTGTTGTTGAAAA+TGG + chr1_3:8365960-8365979 None:intergenic 25.0%
! TCAATACTATAGTCAAAGAA+AGG + chr1_3:8366040-8366059 None:intergenic 25.0%
!! ATGTATCTTTTAATCTCAGA+TGG - chr1_3:8366243-8366262 Msa0101720:intron 25.0%
!!! TCTTATTTTGTTCTAGAATG+TGG - chr1_3:8366542-8366561 Msa0101720:intron 25.0%
!! AACAAGTTGTTGTTGAAAAT+GGG + chr1_3:8365959-8365978 None:intergenic 25.0%
!! TAACAAGTTGTTGTTGAAAA+TGG + chr1_3:8365960-8365979 None:intergenic 25.0%
! TCAATACTATAGTCAAAGAA+AGG + chr1_3:8366040-8366059 None:intergenic 25.0%
!! ATGTATCTTTTAATCTCAGA+TGG - chr1_3:8366243-8366262 Msa0101720:intron 25.0%
!!! TCTTATTTTGTTCTAGAATG+TGG - chr1_3:8366542-8366561 Msa0101720:intron 25.0%
TGAAATGGATGAACTGAAAA+TGG + chr1_3:8365918-8365937 None:intergenic 30.0%
TGTTTCATTTAGAAGATGGA+AGG + chr1_3:8366008-8366027 None:intergenic 30.0%
TGATTTCAGCAATGATTTAC+AGG + chr1_3:8366188-8366207 None:intergenic 30.0%
AGATGATACTGTTTACTTCA+CGG - chr1_3:8366391-8366410 Msa0101720:intron 30.0%
TTCCTTGTCTTTAAGTACAA+TGG - chr1_3:8366461-8366480 Msa0101720:intron 30.0%
TGAAATGGATGAACTGAAAA+TGG + chr1_3:8365918-8365937 None:intergenic 30.0%
TGTTTCATTTAGAAGATGGA+AGG + chr1_3:8366008-8366027 None:intergenic 30.0%
TGATTTCAGCAATGATTTAC+AGG + chr1_3:8366188-8366207 None:intergenic 30.0%
AGATGATACTGTTTACTTCA+CGG - chr1_3:8366391-8366410 Msa0101720:intron 30.0%
TTCCTTGTCTTTAAGTACAA+TGG - chr1_3:8366461-8366480 Msa0101720:intron 30.0%
TACTCAGAAATTGGGTGAAA+TGG + chr1_3:8365933-8365952 None:intergenic 35.0%
! AAGTTGTTGTTGAAAATGGG+TGG + chr1_3:8365956-8365975 None:intergenic 35.0%
GAACTAACAAGTGCAGTTAA+AGG + chr1_3:8366112-8366131 None:intergenic 35.0%
CACCATTGTACTTAAAGACA+AGG + chr1_3:8366466-8366485 None:intergenic 35.0%
TTCAAAGAATTCTGTCAGAG+AGG - chr1_3:8366604-8366623 Msa0101720:CDS 35.0%
TACTCAGAAATTGGGTGAAA+TGG + chr1_3:8365933-8365952 None:intergenic 35.0%
! AAGTTGTTGTTGAAAATGGG+TGG + chr1_3:8365956-8365975 None:intergenic 35.0%
GAACTAACAAGTGCAGTTAA+AGG + chr1_3:8366112-8366131 None:intergenic 35.0%
CACCATTGTACTTAAAGACA+AGG + chr1_3:8366466-8366485 None:intergenic 35.0%
TTCAAAGAATTCTGTCAGAG+AGG - chr1_3:8366604-8366623 Msa0101720:CDS 35.0%
ATGGGTGGTACTCAGAAATT+GGG + chr1_3:8365941-8365960 None:intergenic 40.0%
AATGGGTGGTACTCAGAAAT+TGG + chr1_3:8365942-8365961 None:intergenic 40.0%
!! CCTTTAAGGGTCACATTTTC+TGG + chr1_3:8366332-8366351 None:intergenic 40.0%
CCAGAAAATGTGACCCTTAA+AGG - chr1_3:8366329-8366348 Msa0101720:intron 40.0%
AACTCCACTAGGACCTTTAA+GGG + chr1_3:8366345-8366364 None:intergenic 40.0%
GATACTGTTTACTTCACGGA+CGG - chr1_3:8366395-8366414 Msa0101720:intron 40.0%
CAATGGTGAATCACTCTTTG+AGG - chr1_3:8366478-8366497 Msa0101720:intron 40.0%
GACAAGCTCATACTGAACAA+GGG - chr1_3:8366564-8366583 Msa0101720:CDS 40.0%
ACAAGCTCATACTGAACAAG+GGG - chr1_3:8366565-8366584 Msa0101720:CDS 40.0%
ATGGGTGGTACTCAGAAATT+GGG + chr1_3:8365941-8365960 None:intergenic 40.0%
AATGGGTGGTACTCAGAAAT+TGG + chr1_3:8365942-8365961 None:intergenic 40.0%
!! CCTTTAAGGGTCACATTTTC+TGG + chr1_3:8366332-8366351 None:intergenic 40.0%
CCAGAAAATGTGACCCTTAA+AGG - chr1_3:8366329-8366348 Msa0101720:intron 40.0%
AACTCCACTAGGACCTTTAA+GGG + chr1_3:8366345-8366364 None:intergenic 40.0%
GATACTGTTTACTTCACGGA+CGG - chr1_3:8366395-8366414 Msa0101720:intron 40.0%
CAATGGTGAATCACTCTTTG+AGG - chr1_3:8366478-8366497 Msa0101720:intron 40.0%
GACAAGCTCATACTGAACAA+GGG - chr1_3:8366564-8366583 Msa0101720:CDS 40.0%
ACAAGCTCATACTGAACAAG+GGG - chr1_3:8366565-8366584 Msa0101720:CDS 40.0%
CATGGGAGGAAGAGATTTAC+TGG - chr1_3:8365890-8365909 Msa0101720:CDS 45.0%
CAACTCCACTAGGACCTTTA+AGG + chr1_3:8366346-8366365 None:intergenic 45.0%
ATATCCCACACAACTCCACT+AGG + chr1_3:8366356-8366375 None:intergenic 45.0%
AGTGGAGTTGTGTGGGATAT+TGG - chr1_3:8366356-8366375 Msa0101720:intron 45.0%
GTGGAGTTGTGTGGGATATT+GGG - chr1_3:8366357-8366376 Msa0101720:intron 45.0%
GGACAAGCTCATACTGAACA+AGG - chr1_3:8366563-8366582 Msa0101720:CDS 45.0%
CAAGCTCATACTGAACAAGG+GGG - chr1_3:8366566-8366585 Msa0101720:CDS 45.0%
ACTGAACAAGGGGGTAACAA+AGG - chr1_3:8366575-8366594 Msa0101720:CDS 45.0%
CATGGGAGGAAGAGATTTAC+TGG - chr1_3:8365890-8365909 Msa0101720:CDS 45.0%
CAACTCCACTAGGACCTTTA+AGG + chr1_3:8366346-8366365 None:intergenic 45.0%
ATATCCCACACAACTCCACT+AGG + chr1_3:8366356-8366375 None:intergenic 45.0%
AGTGGAGTTGTGTGGGATAT+TGG - chr1_3:8366356-8366375 Msa0101720:intron 45.0%
GTGGAGTTGTGTGGGATATT+GGG - chr1_3:8366357-8366376 Msa0101720:intron 45.0%
GGACAAGCTCATACTGAACA+AGG - chr1_3:8366563-8366582 Msa0101720:CDS 45.0%
CAAGCTCATACTGAACAAGG+GGG - chr1_3:8366566-8366585 Msa0101720:CDS 45.0%
ACTGAACAAGGGGGTAACAA+AGG - chr1_3:8366575-8366594 Msa0101720:CDS 45.0%
TGGGAGGTGGAAGAAGTTCA+TGG - chr1_3:8365872-8365891 Msa0101720:CDS 50.0%
GGGAGGTGGAAGAAGTTCAT+GGG - chr1_3:8365873-8365892 Msa0101720:CDS 50.0%
AGGTGGAAGAAGTTCATGGG+AGG - chr1_3:8365876-8365895 Msa0101720:CDS 50.0%
GTGACCCTTAAAGGTCCTAG+TGG - chr1_3:8366338-8366357 Msa0101720:intron 50.0%
AAGGTCCTAGTGGAGTTGTG+TGG - chr1_3:8366348-8366367 Msa0101720:intron 50.0%
AGGTCCTAGTGGAGTTGTGT+GGG - chr1_3:8366349-8366368 Msa0101720:intron 50.0%
CTGTTTACTTCACGGACGGT+TGG - chr1_3:8366399-8366418 Msa0101720:intron 50.0%
TGGGAGGTGGAAGAAGTTCA+TGG - chr1_3:8365872-8365891 Msa0101720:CDS 50.0%
GGGAGGTGGAAGAAGTTCAT+GGG - chr1_3:8365873-8365892 Msa0101720:CDS 50.0%
AGGTGGAAGAAGTTCATGGG+AGG - chr1_3:8365876-8365895 Msa0101720:CDS 50.0%
GTGACCCTTAAAGGTCCTAG+TGG - chr1_3:8366338-8366357 Msa0101720:intron 50.0%
AAGGTCCTAGTGGAGTTGTG+TGG - chr1_3:8366348-8366367 Msa0101720:intron 50.0%
AGGTCCTAGTGGAGTTGTGT+GGG - chr1_3:8366349-8366368 Msa0101720:intron 50.0%
CTGTTTACTTCACGGACGGT+TGG - chr1_3:8366399-8366418 Msa0101720:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8365871 8366658 8365871 ID=Msa0101720;Name=Msa0101720
chr1_3 mRNA 8365871 8366658 8365871 ID=Msa0101720-mRNA-1;Parent=Msa0101720;Name=Msa0101720-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|158
chr1_3 exon 8366554 8366658 8366554 ID=Msa0101720-mRNA-1:exon:3375;Parent=Msa0101720-mRNA-1
chr1_3 exon 8365871 8366242 8365871 ID=Msa0101720-mRNA-1:exon:3374;Parent=Msa0101720-mRNA-1
chr1_3 CDS 8366554 8366658 8366554 ID=Msa0101720-mRNA-1:cds;Parent=Msa0101720-mRNA-1
chr1_3 CDS 8365871 8366242 8365871 ID=Msa0101720-mRNA-1:cds;Parent=Msa0101720-mRNA-1
Gene Sequence

>Msa0101720

ATGGGAGGTGGAAGAAGTTCATGGGAGGAAGAGATTTACTGGAACCATTTTCAGTTCATCCATTTCACCCAATTTCTGAGTACCACCCATTTTCAACAACAACTTGCACTTCCTAAAACATTTTCAGACAATTTAAAAAAAAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGGATATTGGGCTGACAACTAAAGATGATACTGTTTACTTCACGGACGGTTGGCAACGTTTTGTGAAAGATCACTCTTTGAAACAGAATGATTTCCTTGTCTTTAAGTACAATGGTGAATCACTCTTTGAGGTTTTAATCTTTAATGGAGAAAGCTTATGTGAGAAAGCAGCTTCTTATTTTGTTCTAGAATGTGGACAAGCTCATACTGAACAAGGGGGTAACAAAGGAACGAATTCAAAGAATTCTGTCAGAGAGGTCAATACTTGTAATATCCTTAAGAGTAAGTAA

Protein sequence

>Msa0101720

MGGGRSSWEEEIYWNHFQFIHFTQFLSTTHFQQQLALPKTFSDNLKKKLPENVTLKGPSGVVWDIGLTTKDDTVYFTDGWQRFVKDHSLKQNDFLVFKYNGESLFEVLIFNGESLCEKAASYFVLECGQAHTEQGGNKGTNSKNSVREVNTCNILKSK*