AlfalfaGEDB | Msa0115710

M. sativa cultivar zhongmu-4 / Msa0115710

KEGG: -
GO: -
PFAM: PF02893.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0115710	sp\|Q9FMW4\|GEML8_ARATH	58.219	146	61	0	245	390	69	214	5.04e-57	189
Msa0115710	sp\|Q9FMW4\|GEML8_ARATH	46.907	194	97	3	19	210	12	201	2.75e-53	179

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0115710	A0A371GW26	58.696	414	118	7	3	392	250	634	9.07e-156	463
Msa0115710	A0A371GW26	59.080	413	116	8	2	390	59	442	1.35e-150	450

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0104670	Msa0115710	0.810784	9.701873e-51	-8.615850e-47
Msa0115710	Msa0241140	0.800885	1.168117e-48	-8.615850e-47
Msa0115710	Msa0817460	0.800851	1.187019e-48	-8.615850e-47
Msa0115710	Msa1325410	-0.806655	7.404369e-50	-8.615850e-47
Msa0115710	Msa1416180	0.848939	4.451102e-60	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0115710	MtrunA17_Chr1g0212171	89.952	209	17	1	1	209	1	205	2.65e-131	376
Msa0115710	MtrunA17_Chr1g0212171	84.916	179	23	1	218	396	51	225	1.04e-106	313
Msa0115710	MtrunA17_Chr1g0212181	78.469	209	38	2	1	209	1	202	4.99e-112	327
Msa0115710	MtrunA17_Chr1g0212181	84.946	186	24	1	219	404	49	230	4.31e-111	325
Msa0115710	MtrunA17_Chr1g0212121	75.238	210	45	2	1	210	1	203	1.17e-109	321
Msa0115710	MtrunA17_Chr1g0212121	77.143	175	36	1	218	392	48	218	3.44e-93	279
Msa0115710	MtrunA17_Chr1g0162171	57.219	187	66	2	216	392	41	223	4.29e-68	215
Msa0115710	MtrunA17_Chr1g0162171	51.852	216	83	4	5	210	4	208	9.33e-68	214
Msa0115710	MtrunA17_Chr1g0212091	53.052	213	90	5	1	210	1	206	6.47e-68	214
Msa0115710	MtrunA17_Chr1g0212091	57.738	168	67	1	226	393	59	222	2.10e-65	208

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0115710	AT5G23370.1	58.219	146	61	0	245	390	69	214	5.12e-58	189
Msa0115710	AT5G23370.1	46.907	194	97	3	19	210	12	201	2.80e-54	179
Msa0115710	AT5G08350.1	50.794	189	81	3	209	390	30	213	7.75e-58	189
Msa0115710	AT5G08350.1	48.352	182	89	3	30	210	23	200	1.72e-52	175
Msa0115710	AT5G23350.1	56.849	146	63	0	245	390	69	214	3.76e-56	184
Msa0115710	AT5G23350.1	50.000	170	78	2	58	224	53	218	1.64e-52	175
Msa0115710	AT5G23360.1	53.741	147	68	0	245	391	61	207	7.18e-54	178
Msa0115710	AT5G23360.1	47.802	182	83	4	30	210	23	193	4.78e-50	168
Msa0115710	AT5G13200.1	40.136	147	88	0	244	390	115	261	8.90e-33	124
Msa0115710	AT5G13200.1	36.424	151	92	1	60	210	102	248	4.29e-29	114

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Find 83 sgRNAs with CRISPR-Local

Find 320 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGGACATTGAGCTTAG+GGG	0.650107	1_3:-34646838	Msa0115710:CDS
AAAGAGGACGTTGAGCTTAG+GGG	0.634036	1_3:-34643116	Msa0115710:CDS
AACTTTCAAATATCTCGAGC+AGG	0.543455	1_3:-34642657	Msa0115710:CDS
AAGATTTGTGTTTGGTTGAA+AGG	0.405842	1_3:+34647325	None:intergenic
AAGGGTACTCACCATGTTCT+TGG	0.303202	1_3:+34646990	None:intergenic
AATGCGTAGGATCCAATATA+AGG	0.416880	1_3:-34642894	Msa0115710:intron
AATGTGTAGGATCCGCTATA+AGG	0.514153	1_3:-34646616	Msa0115710:intron
AATTCCAACCAGCAAAAGCG+CGG	0.640219	1_3:-34643367	Msa0115710:CDS
AATTCTTCAAATGGGTGGAG+TGG	0.590708	1_3:-34646811	Msa0115710:CDS
AATTCTTCAAGTAGGTGGAG+TGG	0.608118	1_3:-34643089	Msa0115710:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTACATACTTAGACTACAA+AGG	-	chr1_3:34642223-34642242	Msa0115710:three_prime_UTR	30.0%
CTACATACTTAGACTACAAA+GGG	-	chr1_3:34642224-34642243	Msa0115710:three_prime_UTR	30.0%
AAGATTTGTGTTTGGTTGAA+AGG	+	chr1_3:34642323-34642342	None:intergenic	30.0%
CTTCTTCATGACATAGTTAT+TGG	-	chr1_3:34642372-34642391	Msa0115710:three_prime_UTR	30.0%
ATAATGTGCTTCAATGTTTC+AGG	-	chr1_3:34642572-34642591	Msa0115710:three_prime_UTR	30.0%
GTGAAAAATTTCAGTGAGAT+CGG	-	chr1_3:34642762-34642781	Msa0115710:CDS	30.0%
TGAAAAATTTCAGTGAGATC+GGG	-	chr1_3:34642763-34642782	Msa0115710:CDS	30.0%
AACTTTCAAATATATCGAGC+AGG	-	chr1_3:34643270-34643289	Msa0115710:intron	30.0%
ATGAAGGGAGATTATTGTTA+TGG	-	chr1_3:34643648-34643667	Msa0115710:intron	30.0%
TTGTTATGGTTGAAAAAGGA+TGG	-	chr1_3:34643662-34643681	Msa0115710:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	34642208	34647459	34642208	ID=Msa0115710;Name=Msa0115710
chr1_3	mRNA	34642208	34647459	34642208	ID=Msa0115710-mRNA-1;Parent=Msa0115710;Name=Msa0115710-mRNA-1;_AED=0.23;_eAED=0.23;_QI=152\|0.57\|0.62\|1\|0.85\|0.75\|8\|358\|404
chr1_3	exon	34642208	34642451	34642208	ID=Msa0115710-mRNA-1:exon:10974;Parent=Msa0115710-mRNA-1
chr1_3	exon	34642490	34642813	34642490	ID=Msa0115710-mRNA-1:exon:10973;Parent=Msa0115710-mRNA-1
chr1_3	exon	34642895	34643169	34642895	ID=Msa0115710-mRNA-1:exon:10972;Parent=Msa0115710-mRNA-1
chr1_3	exon	34643314	34643414	34643314	ID=Msa0115710-mRNA-1:exon:10971;Parent=Msa0115710-mRNA-1
chr1_3	exon	34646407	34646531	34646407	ID=Msa0115710-mRNA-1:exon:10970;Parent=Msa0115710-mRNA-1
chr1_3	exon	34646617	34646891	34646617	ID=Msa0115710-mRNA-1:exon:10969;Parent=Msa0115710-mRNA-1
chr1_3	exon	34646990	34647073	34646990	ID=Msa0115710-mRNA-1:exon:10968;Parent=Msa0115710-mRNA-1
chr1_3	exon	34647163	34647459	34647163	ID=Msa0115710-mRNA-1:exon:10967;Parent=Msa0115710-mRNA-1
chr1_3	five_prime_UTR	34647308	34647459	34647308	ID=Msa0115710-mRNA-1:five_prime_utr;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34647163	34647307	34647163	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34646990	34647073	34646990	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34646617	34646891	34646617	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34646407	34646531	34646407	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34643314	34643414	34643314	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34642895	34643169	34642895	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	CDS	34642604	34642813	34642604	ID=Msa0115710-mRNA-1:cds;Parent=Msa0115710-mRNA-1
chr1_3	three_prime_UTR	34642490	34642603	34642490	ID=Msa0115710-mRNA-1:three_prime_utr;Parent=Msa0115710-mRNA-1
chr1_3	three_prime_UTR	34642208	34642451	34642208	ID=Msa0115710-mRNA-1:three_prime_utr;Parent=Msa0115710-mRNA-1

Gene Sequence

>Msa0115710

ATGAAGACTTCACTTCTTCATGACATAGTTATTGGAACCACAATCATCTCAGCAACATATGATCAAATTCAACAGTCGGTTAACAGATACTTACTTGATTCTGCCACTAATCAATGTCAATATCAAACCAAACACCAATGCAAAAGTAGAGCAAATTCTAACCAGGAAAAGCGCTGCAGGAAGGCCGATAGTCTCTCACAAAGAGTCCAAGAACATGGTGAGTACCCTTTGAGATCGGGAGCAAACATATCTAAAACAATAAAGAGGACATTGAGCTTAGGGGCTCAAATTCTTCAAATGGGTGGAGTGGAGAAAGCTTTTATGCAGTATTTTAGTGTGACAGAAGGGGAAAGGCTGTTAAAAGTTTCACAATGTTATCTCTCCACCACATCTGGTCCTCTATCTGGTCTCCTCTTCATCTCCAATGAAAAGGTTGCCTTCTGCAGTGAGAGAACAATAAAAGTCTTCAATCAGAAAGGTCAAATGTGTAGGATCCGCTATAAGGTTGCCATTCCATTGAAGAAGATCAAGTGTGTGAGGCAAAGTCAAAATGTTGAGAAGTCAACACAAAAGTACATAAACATAGTCACAGTCGACAATTTTGACTTCTGGCTTATGGGTGTCCTAAATGCGCTTAACGTTTCAGCTAGTGCAAATTCCAACCAGCAAAAGCGCGGCAGAACAGCTGTTAATCTCACACAAAGAGTCCAAGAACATGGTGAGTACCCCTTGAGACTCGGAGCAAACATATCTAAAACAATAAAGAGGACGTTGAGCTTAGGGGCTCAAATTCTTCAAGTAGGTGGAGTGGAGAAAGTGTTTACGCAGTATTTTAGTGTGAGAGAAGGAGAGAGGCTATCAAAAGTTTCGCAGTGTTATCTCTCCACCACATCCGGTCCTCTAGCTGGTCTCCTCTTCATCTCCAATGAAAAGGTTGCCTTCTGCAGTGAGAGAACAATAAAAGTCTTCAATCAGAAAGGTCAAATGCGTAGGATCCAATATAAGGTGGCCATTCCACTGAAGAATATCAAGTGTGTGAATGAAAGTCAAAATGTTGAGAAGCCAACACAAAAGTACATAAACATAGTTACAGAAGACGATTTTGATTTCTGGCTTATGGGAGTCCTAAAATATAAAAAAACTTTCAAATATCTCGAGCAGGCAGTTTCTCAAGCAAGCTTAGATGAATATAACAAGTCCACTATTAGAGCATAA

Protein sequence

>Msa0115710

MKTSLLHDIVIGTTIISATYDQIQQSVNRYLLDSATNQCQYQTKHQCKSRANSNQEKRCRKADSLSQRVQEHGEYPLRSGANISKTIKRTLSLGAQILQMGGVEKAFMQYFSVTEGERLLKVSQCYLSTTSGPLSGLLFISNEKVAFCSERTIKVFNQKGQMCRIRYKVAIPLKKIKCVRQSQNVEKSTQKYINIVTVDNFDFWLMGVLNALNVSASANSNQQKRGRTAVNLTQRVQEHGEYPLRLGANISKTIKRTLSLGAQILQVGGVEKVFTQYFSVREGERLSKVSQCYLSTTSGPLAGLLFISNEKVAFCSERTIKVFNQKGQMRRIQYKVAIPLKNIKCVNESQNVEKPTQKYINIVTEDDFDFWLMGVLKYKKTFKYLEQAVSQASLDEYNKSTIRA*