AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0122010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122010 MtrunA17_Chr1g0181581 97.727 440 10 0 6 445 27 466 0.0 886
Msa0122010 MtrunA17_Chr7g0263731 70.721 444 112 9 14 444 29 467 0.0 562
Msa0122010 MtrunA17_Chr6g0462191 48.157 407 161 11 42 430 52 426 9.07e-95 292
Msa0122010 MtrunA17_Chr1g0200161 43.784 370 166 11 91 442 137 482 9.25e-79 252
Msa0122010 MtrunA17_Chr4g0045641 30.997 371 201 9 74 415 130 474 1.48e-36 140
Msa0122010 MtrunA17_Chr8g0346021 29.870 385 210 11 80 414 112 486 2.28e-35 137
Msa0122010 MtrunA17_Chr4g0025441 40.310 258 138 7 9 254 2 255 3.14e-35 135
Msa0122010 MtrunA17_Chr4g0020671 29.798 396 194 15 11 392 30 355 5.23e-30 120
Msa0122010 MtrunA17_Chr2g0283321 38.922 167 90 5 94 255 62 221 8.47e-29 114
Msa0122010 MtrunA17_Chr2g0316401 35.758 165 96 3 96 255 75 234 3.26e-27 110
Msa0122010 MtrunA17_Chr3g0135851 41.667 204 96 7 88 271 78 278 2.05e-26 110
Msa0122010 MtrunA17_Chr7g0273861 33.728 169 97 4 94 258 124 281 2.54e-24 104
Msa0122010 MtrunA17_Chr3g0126901 29.154 319 160 12 95 405 112 372 4.70e-21 95.1
Msa0122010 MtrunA17_Chr1g0146931 37.037 189 92 4 94 255 121 309 7.93e-21 94.7
Msa0122010 MtrunA17_Chr1g0204901 40.816 98 51 1 90 180 131 228 9.67e-16 79.0
Msa0122010 MtrunA17_Chr1g0203711 27.742 310 172 9 94 389 114 385 7.41e-15 76.3
Msa0122010 MtrunA17_Chr8g0389781 53.731 67 31 0 95 161 126 192 1.50e-14 75.5
Msa0122010 MtrunA17_Chr1g0172611 35.036 137 81 1 90 218 100 236 2.05e-14 74.3
Msa0122010 MtrunA17_Chr7g0273951 47.297 74 39 0 97 170 108 181 5.41e-14 73.2
Msa0122010 MtrunA17_Chr6g0472551 41.000 100 56 2 75 171 94 193 7.15e-14 73.2
Msa0122010 MtrunA17_Chr2g0330051 41.176 170 88 5 95 254 78 245 7.89e-14 72.8
Msa0122010 MtrunA17_Chr8g0340761 36.975 119 61 4 94 209 125 232 1.04e-13 72.8
Msa0122010 MtrunA17_Chr4g0060381 50.769 65 32 0 95 159 100 164 1.00e-12 70.1
Msa0122010 MtrunA17_Chr2g0330921 51.562 64 31 0 96 159 98 161 1.03e-12 69.3
Msa0122010 MtrunA17_Chr1g0166431 42.000 100 56 1 95 192 117 216 1.56e-12 69.3
Msa0122010 MtrunA17_Chr8g0342551 37.500 168 95 3 95 255 90 254 3.22e-12 68.2
Msa0122010 MtrunA17_Chr1g0156121 29.560 159 90 2 95 253 124 260 3.54e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0122010 AT5G03040.1 52.273 440 171 15 24 444 41 460 4.06e-125 371
Msa0122010 AT5G03040.3 52.273 440 171 15 24 444 41 460 4.06e-125 371
Msa0122010 AT5G03040.2 52.273 440 171 15 24 444 41 460 4.06e-125 371
Msa0122010 AT3G52290.1 42.825 439 199 14 12 438 29 427 3.95e-92 285
Msa0122010 AT3G09710.1 49.622 397 161 16 61 438 77 453 9.04e-88 275
Msa0122010 AT3G09710.2 47.932 411 161 17 61 438 77 467 1.92e-84 267
Msa0122010 AT2G26410.2 34.831 356 187 8 94 440 147 466 1.58e-45 166
Msa0122010 AT2G26410.1 34.831 356 187 8 94 440 147 466 1.58e-45 166
Msa0122010 AT3G59690.2 33.025 324 166 11 98 413 172 452 7.48e-34 133
Msa0122010 AT3G59690.1 33.025 324 166 11 98 413 172 452 7.48e-34 133
Msa0122010 AT2G43680.4 30.211 331 180 10 92 415 319 605 5.38e-32 129
Msa0122010 AT2G43680.2 30.211 331 180 10 92 415 319 605 5.38e-32 129
Msa0122010 AT2G43680.3 30.211 331 180 10 92 415 319 605 5.38e-32 129
Msa0122010 AT2G43680.5 30.211 331 180 10 92 415 319 605 5.38e-32 129
Msa0122010 AT2G43680.1 30.211 331 180 10 92 415 320 606 5.82e-32 129
Msa0122010 AT3G22190.3 45.833 192 96 5 66 254 63 249 6.39e-31 123
Msa0122010 AT3G22190.2 45.833 192 96 5 66 254 63 249 6.39e-31 123
Msa0122010 AT3G22190.4 45.833 192 96 5 66 254 63 249 6.39e-31 123
Msa0122010 AT3G22190.1 45.833 192 96 5 66 254 63 249 6.39e-31 123
Msa0122010 AT2G33990.1 37.349 166 97 3 93 255 45 206 3.76e-30 118
Msa0122010 AT2G26180.1 45.665 173 80 4 94 255 83 252 1.18e-28 117
Msa0122010 AT4G00820.1 29.261 352 213 9 94 416 131 475 1.86e-28 118
Msa0122010 AT3G15050.1 38.235 170 99 4 89 255 44 210 8.61e-28 111
Msa0122010 AT1G01110.2 27.826 460 242 19 14 416 43 469 1.27e-26 112
Msa0122010 AT3G49260.3 28.616 318 169 8 94 391 119 398 2.71e-25 108
Msa0122010 AT3G49260.1 28.707 317 169 9 94 391 119 397 2.93e-25 108
Msa0122010 AT3G49260.4 28.707 317 169 9 94 391 119 397 2.93e-25 108
Msa0122010 AT3G49260.2 28.707 317 169 9 94 391 119 397 2.93e-25 108
Msa0122010 AT5G13460.1 29.605 304 138 10 96 390 112 348 5.07e-25 107
Msa0122010 AT1G72670.1 36.464 181 106 4 95 267 93 272 1.04e-21 97.1
Msa0122010 AT1G17480.1 36.364 154 86 4 95 246 94 237 5.07e-19 88.6
Msa0122010 AT4G10640.1 26.596 376 166 14 73 412 75 376 3.51e-17 83.6
Msa0122010 AT4G14750.1 44.186 86 48 0 76 161 86 171 1.19e-15 79.0
Msa0122010 AT4G14750.2 44.186 86 48 0 76 161 145 230 1.72e-15 78.6
Msa0122010 AT4G14750.3 44.186 86 48 0 76 161 145 230 1.80e-15 78.6
Msa0122010 AT1G18840.1 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa0122010 AT1G18840.3 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa0122010 AT1G18840.2 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa0122010 AT5G35670.1 32.124 193 110 7 70 254 132 311 1.13e-14 76.3
Msa0122010 AT1G74690.1 32.727 165 82 3 95 255 113 252 2.34e-14 75.5
Msa0122010 AT3G16490.1 50.000 62 31 0 97 158 109 170 3.63e-12 68.2
Msa0122010 AT1G14380.7 32.353 136 78 2 58 179 43 178 1.26e-11 67.0
Msa0122010 AT1G14380.6 32.353 136 78 2 58 179 43 178 1.32e-11 67.0
Msa0122010 AT1G14380.5 32.353 136 78 2 58 179 43 178 1.32e-11 67.0
Msa0122010 AT1G14380.2 32.353 136 78 2 58 179 43 178 1.32e-11 67.0
Msa0122010 AT1G14380.1 32.353 136 78 2 58 179 43 178 1.56e-11 67.0
Msa0122010 AT1G14380.3 32.353 136 78 2 58 179 43 178 1.56e-11 67.0
Msa0122010 AT1G14380.4 32.353 136 78 2 58 179 43 178 1.56e-11 67.0
Msa0122010 AT1G51960.1 32.911 158 92 4 12 158 9 163 3.84e-11 64.7
Msa0122010 AT5G03960.3 33.333 144 64 3 92 218 107 235 6.58e-11 64.3
Msa0122010 AT5G03960.2 33.333 144 64 3 92 218 107 235 6.58e-11 64.3
Msa0122010 AT5G03960.1 33.333 144 64 3 92 218 106 234 6.61e-11 64.3
Msa0122010 AT5G07240.1 50.847 59 29 0 97 155 108 166 7.44e-11 64.3
Msa0122010 AT5G07240.2 50.847 59 29 0 97 155 154 212 9.40e-11 63.9

Find 141 sgRNAs with CRISPR-Local

Find 293 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGCATTTCGGGCTTTA+AGG 0.138542 1_3:-48317654 Msa0122010:CDS
AAGAATATTTGTAGTATATC+TGG 0.187687 1_3:-48314520 Msa0122010:three_prime_UTR
ATCTATAAATCCAATGTTTA+TGG 0.198850 1_3:-48315627 Msa0122010:CDS
GGAGGTGCTTGATCAGTTTC+TGG 0.216889 1_3:+48318186 None:intergenic
AACCTCATCATTCGGTTTAC+TGG 0.224722 1_3:+48317999 None:intergenic
ACCACATTGCCCATGATAAT+TGG 0.235229 1_3:-48318949 None:intergenic
TGATAAGCTACACATAAATT+TGG 0.239726 1_3:+48314864 None:intergenic
AGTTAGGTGAACCCGGATTT+TGG 0.244125 1_3:+48315420 None:intergenic
GAGAACACTACACCAGAAAA+AGG 0.257119 1_3:-48315137 Msa0122010:CDS
TTAGGCAAGAAGAGCATTTC+GGG 0.261895 1_3:-48317662 Msa0122010:intron
TTCAGTTTCTTGACAATTGC+TGG 0.269742 1_3:+48315371 None:intergenic
CTGCACACTCGTCATGCTTT+TGG 0.277740 1_3:+48315307 None:intergenic
TTCTTGACAATTGCTGGCTT+GGG 0.292014 1_3:+48315377 None:intergenic
TCCAAAGGTGAGCTGTATTA+TGG 0.294106 1_3:-48315339 Msa0122010:CDS
TTTGAGTTGGATTGGAAGTT+AGG 0.295442 1_3:+48315404 None:intergenic
TTTAAGCTGCTTTCTACCTT+TGG 0.311790 1_3:+48315038 None:intergenic
TGACCCGCCTCGAACGAATC+TGG 0.328605 1_3:+48317533 None:intergenic
AAAGAAACGGCTTAACTTCC+CGG 0.332661 1_3:-48315099 Msa0122010:CDS
TTTCTTGACAATTGCTGGCT+TGG 0.333380 1_3:+48315376 None:intergenic
TTTAGGCAAGAAGAGCATTT+CGG 0.342976 1_3:-48317663 Msa0122010:intron
TCTCCTTCTCCATCAGGCTT+TGG 0.344634 1_3:+48315549 None:intergenic
TCAAGCCGCTCCAATTGTTC+AGG 0.350981 1_3:-48318025 Msa0122010:CDS
ATCTCGAGAGCATTCCCATT+TGG 0.351037 1_3:-48314715 Msa0122010:three_prime_UTR
TCTCGAGAGCATTCCCATTT+GGG 0.354296 1_3:-48314714 Msa0122010:three_prime_UTR
CGGAGGAGGCACTCCATTGC+TGG 0.355945 1_3:-48315272 Msa0122010:CDS
CTCGTACTTGCTTAGTAACT+TGG 0.364044 1_3:+48317260 None:intergenic
GGCTTATTCATTCACTCATC+AGG 0.365734 1_3:-48317211 Msa0122010:intron
TGTGTAGCTTATCATTTCCT+TGG 0.374153 1_3:-48314855 Msa0122010:three_prime_UTR
TCCAACAGCGAGCCAAAATC+CGG 0.381133 1_3:-48315432 Msa0122010:CDS
GGATCAAAGAAGAAATGGTT+TGG 0.384554 1_3:-48318240 Msa0122010:CDS
AATTGTTCTCCTTCTCCATC+AGG 0.385930 1_3:+48315543 None:intergenic
AATTGTCAAGAAACTGAATA+AGG 0.387705 1_3:-48315366 Msa0122010:CDS
CAAGTGGATCAAAGAAGAAA+TGG 0.394536 1_3:-48318245 Msa0122010:CDS
AAGCTGCTTTCTACCTTTGG+TGG 0.401382 1_3:+48315041 None:intergenic
CTTCTCCATCAGGCTTTGGT+TGG 0.409739 1_3:+48315553 None:intergenic
GAACAGGTTGAGAGCACTAA+TGG 0.410177 1_3:-48317626 Msa0122010:CDS
TGAATGCCGCCTTGATGATC+TGG 0.413022 1_3:+48315067 None:intergenic
TGGGAGGCTTTGAGTTGGAT+TGG 0.416717 1_3:+48315396 None:intergenic
TTACTGGCAACCTGAACAAT+TGG 0.421900 1_3:+48318015 None:intergenic
TGGACTTTGCAACCTGGACT+TGG 0.423630 1_3:+48315169 None:intergenic
GATGATCTGGCAGGCGAAGC+CGG 0.424668 1_3:+48315080 None:intergenic
GGATCCAACGAATCCTAGTT+GGG 0.426047 1_3:-48315606 Msa0122010:CDS
TCTGTTTGAACATCAGAAAC+AGG 0.427072 1_3:+48318060 None:intergenic
AGATCATCAAGGCGGCATTC+AGG 0.428105 1_3:-48315065 Msa0122010:CDS
AGCTGCTTTCTACCTTTGGT+GGG 0.428437 1_3:+48315042 None:intergenic
AAGAGCATTTCGGGCTTTAA+GGG 0.430354 1_3:-48317653 Msa0122010:CDS
CTCCGCTCTATGCAGACTTT+AGG 0.430493 1_3:-48317568 Msa0122010:CDS
CACAAAAGAGCTAGAGATAC+AGG 0.432698 1_3:-48317461 Msa0122010:intron
GTAGCTTATCATTTCCTTGG+AGG 0.441460 1_3:-48314852 Msa0122010:three_prime_UTR
TTGACAATTGCTGGCTTGGG+AGG 0.449771 1_3:+48315380 None:intergenic
TGATTTGCTGATTTCAGCAC+TGG 0.452185 1_3:+48315490 None:intergenic
GTGTGCAGTCGGAGCGTGTT+CGG 0.453061 1_3:-48315292 Msa0122010:CDS
TTAGTTGGCACCATGTAACT+TGG 0.454849 1_3:+48315203 None:intergenic
AGATTCGTTCGAGGCGGGTC+AGG 0.459187 1_3:-48317531 Msa0122010:CDS
TCCATAATACAGCTCACCTT+TGG 0.459798 1_3:+48315338 None:intergenic
AGAATGATAACAGTCCCAAA+TGG 0.464056 1_3:+48314700 None:intergenic
GATCAAAGAAGAAATGGTTT+GGG 0.464945 1_3:-48318239 Msa0122010:CDS
TCAGCCTCAGGTGGATGAAA+AGG 0.467361 1_3:+48318165 None:intergenic
GACCCGCCTCGAACGAATCT+GGG 0.469265 1_3:+48317534 None:intergenic
ATTGCCGCAACCTCATCATT+CGG 0.473059 1_3:+48317991 None:intergenic
CATAACAGATTGGGGAGGAA+TGG 0.475348 1_3:-48317317 Msa0122010:intron
TGGATCCAACGAATCCTAGT+TGG 0.479559 1_3:-48315607 Msa0122010:CDS
ATAACAGATTGGGGAGGAAT+GGG 0.482482 1_3:-48317316 Msa0122010:intron
TGGGACCAGTCCAATTATCA+TGG 0.484592 1_3:+48318939 None:intergenic
TATTCATTTGCTTACTAATG+TGG 0.485180 1_3:-48314788 Msa0122010:three_prime_UTR
ATGATCTGGCAGGCGAAGCC+GGG 0.489921 1_3:+48315081 None:intergenic
ATAATCTCCTCCTCAGCCTC+AGG 0.493239 1_3:+48318153 None:intergenic
TTCGTTGGATCCATAAACAT+TGG 0.495801 1_3:+48315617 None:intergenic
GTAGTGTTCTCTGCAGCTGA+TGG 0.498156 1_3:+48315149 None:intergenic
AGGGCCAGTCGTAAAACGTC+AGG 0.499673 1_3:-48317602 Msa0122010:CDS
TGGCTTGGGAGGCTTTGAGT+TGG 0.501361 1_3:+48315391 None:intergenic
GCAAAGTACTCCACTAGTAA+TGG 0.501618 1_3:-48314955 Msa0122010:three_prime_UTR
AGTCGGAGCGTGTTCGGAGG+AGG 0.502604 1_3:-48315286 Msa0122010:CDS
TATGTGTGTTGTGTGTGGCT+TGG 0.505216 1_3:-48314555 Msa0122010:three_prime_UTR
CTTACTAATGTGGTTCATGA+TGG 0.505740 1_3:-48314778 Msa0122010:three_prime_UTR
AGAATATTTGTAGTATATCT+GGG 0.513307 1_3:-48314519 Msa0122010:three_prime_UTR
GAATGATAACAGTCCCAAAT+GGG 0.514412 1_3:+48314701 None:intergenic
GGTACATCAACATCCACATC+TGG 0.515322 1_3:+48318081 None:intergenic
CATCCCCAACTAGGATTCGT+TGG 0.524622 1_3:+48315602 None:intergenic
GAGGCGGGTCAGGATGTTAG+AGG 0.525201 1_3:-48317521 Msa0122010:CDS
CCTTTGGAAATGCAAAGAAA+CGG 0.526599 1_3:-48315112 Msa0122010:CDS
ATCCCAGATTCGTTCGAGGC+GGG 0.527771 1_3:-48317536 Msa0122010:CDS
TACCAAGTATCGCCGAAAAG+TGG 0.530850 1_3:+48317957 None:intergenic
TTAAATGCAGAAATTACTGT+TGG 0.533004 1_3:-48315020 Msa0122010:CDS
ATCCTAGTTGGGGATGGAGC+TGG 0.536093 1_3:-48315595 Msa0122010:CDS
TGCCGCCTTGATGATCTGGC+AGG 0.546459 1_3:+48315071 None:intergenic
AGCTGATGGACTTTGCAACC+TGG 0.547253 1_3:+48315163 None:intergenic
TCATCCACCTGAGGCTGAGG+AGG 0.549283 1_3:-48318160 Msa0122010:CDS
CAACCAAAGCCTGATGGAGA+AGG 0.551822 1_3:-48315552 Msa0122010:CDS
AGAATCACAAATAAGAAATG+AGG 0.552097 1_3:+48314628 None:intergenic
CCGTTTCTTTGCATTTCCAA+AGG 0.555247 1_3:+48315112 None:intergenic
GATTGGAAGTTAGGTGAACC+CGG 0.557782 1_3:+48315413 None:intergenic
AGTAAAGAAAACAGCCTCCA+AGG 0.558637 1_3:+48314838 None:intergenic
TTTGTAGTATATCTGGGAAG+TGG 0.565012 1_3:-48314513 Msa0122010:three_prime_UTR
TCCATCAGTTCCAAGTTACA+TGG 0.565302 1_3:-48315213 Msa0122010:CDS
TGCAATCCCAGATTCGTTCG+AGG 0.569562 1_3:-48317540 Msa0122010:CDS
TAAACCGAATGATGAGGTTG+CGG 0.570682 1_3:-48317995 Msa0122010:CDS
GGCAGCCAACCAAAGCCTGA+TGG 0.570992 1_3:-48315558 Msa0122010:CDS
CAACGAATCCTAGTTGGGGA+TGG 0.576539 1_3:-48315601 Msa0122010:CDS
ACCATGTAACTTGGAACTGA+TGG 0.577244 1_3:+48315212 None:intergenic
AACCAGCTCCATCCCCAACT+AGG 0.580335 1_3:+48315593 None:intergenic
AGAGCATTTCGGGCTTTAAG+GGG 0.582500 1_3:-48317652 Msa0122010:CDS
ATCTCCTCCTCAGCCTCAGG+TGG 0.583117 1_3:+48318156 None:intergenic
TGATATAATTCCATTACTAG+TGG 0.586145 1_3:+48314945 None:intergenic
AGTCTGCAAAAGCCAAGTCC+AGG 0.592138 1_3:-48315181 Msa0122010:CDS
CTTCGCCTGCCAGATCATCA+AGG 0.592606 1_3:-48315076 Msa0122010:CDS
CCAACAGCGAGCCAAAATCC+GGG 0.594662 1_3:-48315431 Msa0122010:CDS
TGCAGTCGGAGCGTGTTCGG+AGG 0.597571 1_3:-48315289 Msa0122010:CDS
GAGCTGGTTAGAACGTTGGA+TGG 0.602368 1_3:-48315579 Msa0122010:CDS
ATTGCCCATGATAATTGGAC+TGG 0.603587 1_3:-48318944 None:intergenic
AGGTTGAGAGCACTAATGGA+AGG 0.610005 1_3:-48317622 Msa0122010:CDS
GATGGAGCTGGTTAGAACGT+TGG 0.610918 1_3:-48315583 Msa0122010:CDS
AAGCATGACGAGTGTGCAGT+CGG 0.612598 1_3:-48315303 Msa0122010:CDS
GCCTCAGGTGGATGAAAAGG+AGG 0.614504 1_3:+48318168 None:intergenic
GGGACCAGTCCAATTATCAT+GGG 0.621023 1_3:+48318940 None:intergenic
CCTCACTATTGATCCAGCAA+TGG 0.623634 1_3:+48315259 None:intergenic
ACTGAATAAGGCTAGTCCAA+AGG 0.624104 1_3:-48315354 Msa0122010:CDS
CTGTTTGATTATATAACCTG+AGG 0.625685 1_3:-48314986 Msa0122010:exon
GAGCATTTCGGGCTTTAAGG+GGG 0.629409 1_3:-48317651 Msa0122010:CDS
CTAAAGTCTGCATAGAGCGG+AGG 0.629714 1_3:+48317569 None:intergenic
CATTGCTGGATCAATAGTGA+GGG 0.633530 1_3:-48315258 Msa0122010:CDS
TAAAGTCTGCATAGAGCGGA+GGG 0.635485 1_3:+48317570 None:intergenic
TGTTGAAGATGTACCAGATG+TGG 0.637134 1_3:-48318094 Msa0122010:CDS
TATGATATGTGTGTTGTGTG+TGG 0.637756 1_3:-48314560 Msa0122010:three_prime_UTR
GACCTAAAGTCTGCATAGAG+CGG 0.641744 1_3:+48317566 None:intergenic
TGTTCAAACAGAAACTGTTG+AGG 0.641908 1_3:-48318049 Msa0122010:CDS
GGTTGAGAGCACTAATGGAA+GGG 0.642674 1_3:-48317621 Msa0122010:CDS
CCATTGCTGGATCAATAGTG+AGG 0.642996 1_3:-48315259 Msa0122010:CDS
GCGGCATTCAGGCCCACCAA+AGG 0.652957 1_3:-48315054 Msa0122010:CDS
GCAGAAATTACTGTTGGCAA+TGG 0.656114 1_3:-48315014 Msa0122010:CDS
CATAGAGCGGAGGGTACTAA+CGG 0.661136 1_3:+48317579 None:intergenic
TATGAGAAGAGAAAGAGCAA+TGG 0.661509 1_3:-48317232 Msa0122010:CDS
AATCCCAGATTCGTTCGAGG+CGG 0.666163 1_3:-48317537 Msa0122010:CDS
GATCCAACGAATCCTAGTTG+GGG 0.666263 1_3:-48315605 Msa0122010:CDS
CAAGTTACTAAGCAAGTACG+AGG 0.670194 1_3:-48317259 Msa0122010:CDS
TGCCAGTAAACCGAATGATG+AGG 0.671626 1_3:-48318001 Msa0122010:CDS
GGCAACCTGAACAATTGGAG+CGG 0.673984 1_3:+48318020 None:intergenic
TTTCAGCACTGGTAATGCCG+CGG 0.683035 1_3:+48315501 None:intergenic
CGCCTGCCAGATCATCAAGG+CGG 0.686449 1_3:-48315073 Msa0122010:CDS
ATTCTCATAACAGATTGGGG+AGG 0.696674 1_3:-48317322 Msa0122010:intron
AATTACTGTTGGCAATGGTG+TGG 0.715956 1_3:-48315009 Msa0122010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATCAATTTTATAATTG+AGG + chr1_3:48315561-48315580 None:intergenic 10.0%
!! TAGAAATTAATGCAATAATA+TGG - chr1_3:48314842-48314861 Msa0122010:three_prime_UTR 15.0%
!! AGAAATTAATGCAATAATAT+GGG - chr1_3:48314843-48314862 Msa0122010:three_prime_UTR 15.0%
!!! ATTTTGTTTTGATATCATTT+AGG - chr1_3:48315758-48315777 Msa0122010:intron 15.0%
!!! CATAAAAATGTCATTTTTAT+CGG + chr1_3:48316577-48316596 None:intergenic 15.0%
!!! TCATTTTCTATTTCTTTTTT+AGG + chr1_3:48317345-48317364 None:intergenic 15.0%
!! TTTAAACATATATGATTTCT+TGG + chr1_3:48317574-48317593 None:intergenic 15.0%
!!! TAAGTTGAATAAATTGAAGA+GGG + chr1_3:48314773-48314792 None:intergenic 20.0%
!! GAAATTAATGCAATAATATG+GGG - chr1_3:48314844-48314863 Msa0122010:three_prime_UTR 20.0%
!!! AGTTATTTTCATAACGTATT+CGG + chr1_3:48315040-48315059 None:intergenic 20.0%
!!! ATCTTTTGTGATAGAAATTT+AGG - chr1_3:48315094-48315113 Msa0122010:CDS 20.0%
!! ATGTTTAATCTCAATTATCT+GGG - chr1_3:48315132-48315151 Msa0122010:CDS 20.0%
!!! TTTTATTCTGAAGTATATCA+GGG + chr1_3:48316021-48316040 None:intergenic 20.0%
!!! ATTTTATTCTGAAGTATATC+AGG + chr1_3:48316022-48316041 None:intergenic 20.0%
!!! TATGTTTGAATTTTAGCTTA+TGG - chr1_3:48316065-48316084 Msa0122010:intron 20.0%
!!! AAATTTTTGCATATAAAGCA+TGG - chr1_3:48316464-48316483 Msa0122010:intron 20.0%
!! TATGAAAAAAATAAGTGTCA+TGG - chr1_3:48316593-48316612 Msa0122010:intron 20.0%
!!! TTAAATCTTGAGTTATTTTG+AGG + chr1_3:48316639-48316658 None:intergenic 20.0%
!! AATAAAAACAACTTAAAGAG+AGG + chr1_3:48316994-48317013 None:intergenic 20.0%
!!! TTTCTATTTCTTTTTTAGGT+GGG + chr1_3:48317341-48317360 None:intergenic 20.0%
!!! TTTTCTATTTCTTTTTTAGG+TGG + chr1_3:48317342-48317361 None:intergenic 20.0%
!! AAATTATGTTAGCAGAAAAT+AGG + chr1_3:48317688-48317707 None:intergenic 20.0%
!!! TAGAAGTGTATATTTATCTT+AGG + chr1_3:48317760-48317779 None:intergenic 20.0%
!! ATCTATAAATCCAATGTTTA+TGG - chr1_3:48317811-48317830 Msa0122010:intron 20.0%
!! AAGAATATTTGTAGTATATC+TGG - chr1_3:48318918-48318937 Msa0122010:intron 20.0%
!! AGAATATTTGTAGTATATCT+GGG - chr1_3:48318919-48318938 Msa0122010:intron 20.0%
! ATGTGATTAGATTAGATCTT+GGG + chr1_3:48314655-48314674 None:intergenic 25.0%
! AATGTGATTAGATTAGATCT+TGG + chr1_3:48314656-48314675 None:intergenic 25.0%
!! GTAAGTTGAATAAATTGAAG+AGG + chr1_3:48314774-48314793 None:intergenic 25.0%
! CATGTTTAATCTCAATTATC+TGG - chr1_3:48315131-48315150 Msa0122010:CDS 25.0%
! ATTCAACACATAATTGTAAG+AGG + chr1_3:48315516-48315535 None:intergenic 25.0%
! AATGTGAATCCTTACAATTA+AGG - chr1_3:48315601-48315620 Msa0122010:CDS 25.0%
!!! GAATTTTAGCTTATGGATTA+TGG - chr1_3:48316072-48316091 Msa0122010:intron 25.0%
!!! TAAGAAGTTGCATTTGTTTT+AGG - chr1_3:48316264-48316283 Msa0122010:intron 25.0%
! TTTACACATAGTATAAAGCA+TGG - chr1_3:48316382-48316401 Msa0122010:intron 25.0%
!! TTTTTGAACTGAAAGATCTA+AGG - chr1_3:48316413-48316432 Msa0122010:intron 25.0%
! TTTCATGTAATTTACCTTAC+TGG + chr1_3:48316447-48316466 None:intergenic 25.0%
! ACAAGATTTGACTAACAATT+CGG + chr1_3:48316533-48316552 None:intergenic 25.0%
! TTGTTAGTCAAATCTTGTTA+TGG - chr1_3:48316535-48316554 Msa0122010:intron 25.0%
!!! CATTTTGATATTTCATAGCA+TGG + chr1_3:48316921-48316940 None:intergenic 25.0%
!!! TTCTATTTCTTTTTTAGGTG+GGG + chr1_3:48317340-48317359 None:intergenic 25.0%
! CACATCTGATATAAAAATTG+TGG + chr1_3:48317377-48317396 None:intergenic 25.0%
!! AATTTTGTAGATGAAAACTC+AGG + chr1_3:48317550-48317569 None:intergenic 25.0%
! TATTTATCTTAGGTCAAACT+TGG + chr1_3:48317750-48317769 None:intergenic 25.0%
! ACTTCTATTTGACAGAAAAA+CGG - chr1_3:48317773-48317792 Msa0122010:intron 25.0%
! AATTGTCAAGAAACTGAATA+AGG - chr1_3:48318072-48318091 Msa0122010:CDS 25.0%
! TTAAATGCAGAAATTACTGT+TGG - chr1_3:48318418-48318437 Msa0122010:intron 25.0%
! TGATATAATTCCATTACTAG+TGG + chr1_3:48318496-48318515 None:intergenic 25.0%
! TGATAAGCTACACATAAATT+TGG + chr1_3:48318577-48318596 None:intergenic 25.0%
! TATTCATTTGCTTACTAATG+TGG - chr1_3:48318650-48318669 Msa0122010:intron 25.0%
! AGAATCACAAATAAGAAATG+AGG + chr1_3:48318813-48318832 None:intergenic 25.0%
!! TCTTTTATGTTACATTGTTC+TGG - chr1_3:48318850-48318869 Msa0122010:intron 25.0%
GAGAAGTTGAAGATAAAAGA+GGG + chr1_3:48314680-48314699 None:intergenic 30.0%
AGAGAAGTTGAAGATAAAAG+AGG + chr1_3:48314681-48314700 None:intergenic 30.0%
TTCACATTGGAAAACCAATT+TGG + chr1_3:48314879-48314898 None:intergenic 30.0%
!!! TGTTTGTTTTGTTTAGAGTC+AGG + chr1_3:48314915-48314934 None:intergenic 30.0%
CTCTAAACAAAACAAACATC+AGG - chr1_3:48314917-48314936 Msa0122010:three_prime_UTR 30.0%
ATTATCTGGGAAGTTTCTTA+TGG - chr1_3:48315145-48315164 Msa0122010:CDS 30.0%
! CTGATTTTGTAGAATTCAAG+TGG - chr1_3:48315177-48315196 Msa0122010:CDS 30.0%
GATCAAAGAAGAAATGGTTT+GGG - chr1_3:48315199-48315218 Msa0122010:CDS 30.0%
!! TCATGATTCAAACCACTTTT+CGG - chr1_3:48315469-48315488 Msa0122010:CDS 30.0%
CATACACATCCTTAATTGTA+AGG + chr1_3:48315613-48315632 None:intergenic 30.0%
ATAACTTGGCATCATTAAAG+AGG + chr1_3:48315663-48315682 None:intergenic 30.0%
TCCAAATTCTCATAACAGAT+TGG - chr1_3:48316111-48316130 Msa0122010:intron 30.0%
CCAAATTCTCATAACAGATT+GGG - chr1_3:48316112-48316131 Msa0122010:intron 30.0%
CAAATTCTCATAACAGATTG+GGG - chr1_3:48316113-48316132 Msa0122010:intron 30.0%
ATGCAACTTCTTAAGAGAAA+TGG + chr1_3:48316257-48316276 None:intergenic 30.0%
! AAATGAGAAAAGAGCATGTA+TGG - chr1_3:48316301-48316320 Msa0122010:intron 30.0%
TTGATGAACTGAAAGATCAA+AGG - chr1_3:48316328-48316347 Msa0122010:intron 30.0%
CTGAAAGATCAAAGGAAAAA+TGG - chr1_3:48316336-48316355 Msa0122010:intron 30.0%
TATATCTTCTGATTCACGAT+CGG + chr1_3:48316506-48316525 None:intergenic 30.0%
GAAAAGTAGTTAGCTATATG+AGG - chr1_3:48316680-48316699 Msa0122010:intron 30.0%
AAAATGTCTAGCTACTCTTA+AGG - chr1_3:48316712-48316731 Msa0122010:intron 30.0%
TAACGAATCGGAATTTAATG+TGG - chr1_3:48316793-48316812 Msa0122010:intron 30.0%
AACGAATCGGAATTTAATGT+GGG - chr1_3:48316794-48316813 Msa0122010:intron 30.0%
TCCAAAATAGATACACACTT+AGG - chr1_3:48316824-48316843 Msa0122010:intron 30.0%
! TCCTAAGTGTGTATCTATTT+TGG + chr1_3:48316828-48316847 None:intergenic 30.0%
TCTAGTATCAAATCGAGATA+CGG - chr1_3:48316868-48316887 Msa0122010:intron 30.0%
ATATCAAAATGAGAATCCTG+AGG - chr1_3:48316930-48316949 Msa0122010:intron 30.0%
TATCAAAATGAGAATCCTGA+GGG - chr1_3:48316931-48316950 Msa0122010:intron 30.0%
AAAAACAACTTAAAGAGAGG+AGG + chr1_3:48316991-48317010 None:intergenic 30.0%
ACACACAAGCTTAATAACTT+TGG - chr1_3:48317021-48317040 Msa0122010:intron 30.0%
CACACAAGCTTAATAACTTT+GGG - chr1_3:48317022-48317041 Msa0122010:intron 30.0%
!! CTTTTGAGAATTACAGAAAG+TGG - chr1_3:48317165-48317184 Msa0122010:intron 30.0%
TCATGATTCATTAGCAAAAC+TGG + chr1_3:48317245-48317264 None:intergenic 30.0%
GTAGTTTCAGATATATTTGC+CGG + chr1_3:48317282-48317301 None:intergenic 30.0%
CTTACAAAATTAATCGACGT+GGG + chr1_3:48317522-48317541 None:intergenic 30.0%
AAAGATCAAGAAACTCAAAC+TGG + chr1_3:48317712-48317731 None:intergenic 30.0%
CTTCTATTTGACAGAAAAAC+GGG - chr1_3:48317774-48317793 Msa0122010:intron 30.0%
CTGTTTGATTATATAACCTG+AGG - chr1_3:48318452-48318471 Msa0122010:intron 30.0%
AAGAAGAGAGAGAAAGAAGT+GGG + chr1_3:48314576-48314595 None:intergenic 35.0%
AAAGAAGAGAGAGAAAGAAG+TGG + chr1_3:48314577-48314596 None:intergenic 35.0%
TCTCTCTTGCATTTCACTTT+AGG - chr1_3:48314735-48314754 Msa0122010:three_prime_UTR 35.0%
ACTAAGAGCTTTCTTCACAT+TGG + chr1_3:48314892-48314911 None:intergenic 35.0%
ATTCTCACAAGTGACTATGT+CGG + chr1_3:48314980-48314999 None:intergenic 35.0%
CAAGTGGATCAAAGAAGAAA+TGG - chr1_3:48315193-48315212 Msa0122010:CDS 35.0%
GGATCAAAGAAGAAATGGTT+TGG - chr1_3:48315198-48315217 Msa0122010:CDS 35.0%
TCTGTTTGAACATCAGAAAC+AGG + chr1_3:48315381-48315400 None:intergenic 35.0%
TGTTCAAACAGAAACTGTTG+AGG - chr1_3:48315389-48315408 Msa0122010:CDS 35.0%
! CTTTCTTTTGCAGCAATTGT+CGG + chr1_3:48315703-48315722 None:intergenic 35.0%
!! TTTAGGCAAGAAGAGCATTT+CGG - chr1_3:48315775-48315794 Msa0122010:intron 35.0%
CCCAATCTGTTATGAGAATT+TGG + chr1_3:48316115-48316134 None:intergenic 35.0%
TATGAGAAGAGAAAGAGCAA+TGG - chr1_3:48316206-48316225 Msa0122010:intron 35.0%
!!! GCATTTGTTTTAGGTTAGTC+AGG - chr1_3:48316273-48316292 Msa0122010:intron 35.0%
CAAAGGAAAAATGGCTAGTA+AGG - chr1_3:48316345-48316364 Msa0122010:intron 35.0%
CTAAGGAAAAATGACCAGTA+AGG - chr1_3:48316430-48316449 Msa0122010:intron 35.0%
GGGAGAAAATCAAACTTAAG+AGG + chr1_3:48317092-48317111 None:intergenic 35.0%
! TAGCAAAACTGGTTCATACT+AGG + chr1_3:48317234-48317253 None:intergenic 35.0%
!!! TTTCTTTTTTAGGTGGGGTA+GGG + chr1_3:48317335-48317354 None:intergenic 35.0%
!!! ATTTCTTTTTTAGGTGGGGT+AGG + chr1_3:48317336-48317355 None:intergenic 35.0%
ACAAATCCTCTAGTCTACTT+TGG - chr1_3:48317399-48317418 Msa0122010:intron 35.0%
GCTTACAAAATTAATCGACG+TGG + chr1_3:48317523-48317542 None:intergenic 35.0%
TTCGTTGGATCCATAAACAT+TGG + chr1_3:48317824-48317843 None:intergenic 35.0%
! AATGATTTTCGGAATTGAGC+TGG + chr1_3:48317985-48318004 None:intergenic 35.0%
!! TTTGAGTTGGATTGGAAGTT+AGG + chr1_3:48318037-48318056 None:intergenic 35.0%
TTCAGTTTCTTGACAATTGC+TGG + chr1_3:48318070-48318089 None:intergenic 35.0%
AGAACACTACACCAGAAAAA+GGG - chr1_3:48318302-48318321 Msa0122010:intron 35.0%
! CCTTTGGAAATGCAAAGAAA+CGG - chr1_3:48318326-48318345 Msa0122010:intron 35.0%
! TTTAAGCTGCTTTCTACCTT+TGG + chr1_3:48318403-48318422 None:intergenic 35.0%
! TACTTTGCATTCTTTTCCTC+AGG + chr1_3:48318471-48318490 None:intergenic 35.0%
TGTGTAGCTTATCATTTCCT+TGG - chr1_3:48318583-48318602 Msa0122010:intron 35.0%
CTTACTAATGTGGTTCATGA+TGG - chr1_3:48318660-48318679 Msa0122010:intron 35.0%
GAATGATAACAGTCCCAAAT+GGG + chr1_3:48318740-48318759 None:intergenic 35.0%
AGAATGATAACAGTCCCAAA+TGG + chr1_3:48318741-48318760 None:intergenic 35.0%
TATGATATGTGTGTTGTGTG+TGG - chr1_3:48318878-48318897 Msa0122010:intron 35.0%
TTTGTAGTATATCTGGGAAG+TGG - chr1_3:48318925-48318944 Msa0122010:intron 35.0%
AGAAGAGAGAGAAAGAAGTG+GGG + chr1_3:48314575-48314594 None:intergenic 40.0%
TGGGGAAGAATAAGCCAAAT+TGG - chr1_3:48314862-48314881 Msa0122010:three_prime_UTR 40.0%
ATAGTCACTTGTGAGAATGC+TGG - chr1_3:48314982-48315001 Msa0122010:exon 40.0%
TGTTGAAGATGTACCAGATG+TGG - chr1_3:48315344-48315363 Msa0122010:CDS 40.0%
TTACTGGCAACCTGAACAAT+TGG + chr1_3:48315426-48315445 None:intergenic 40.0%
AACCTCATCATTCGGTTTAC+TGG + chr1_3:48315442-48315461 None:intergenic 40.0%
TAAACCGAATGATGAGGTTG+CGG - chr1_3:48315443-48315462 Msa0122010:CDS 40.0%
CCACTAATGACAGCATAACT+TGG + chr1_3:48315677-48315696 None:intergenic 40.0%
CCAAGTTATGCTGTCATTAG+TGG - chr1_3:48315674-48315693 Msa0122010:intron 40.0%
!! TTAGGCAAGAAGAGCATTTC+GGG - chr1_3:48315776-48315795 Msa0122010:intron 40.0%
!! AAGAGCATTTCGGGCTTTAA+GGG - chr1_3:48315785-48315804 Msa0122010:intron 40.0%
CACAAAAGAGCTAGAGATAC+AGG - chr1_3:48315977-48315996 Msa0122010:intron 40.0%
TAGAGATACAGGTCTCTGAA+GGG - chr1_3:48315988-48316007 Msa0122010:intron 40.0%
ATTCTCATAACAGATTGGGG+AGG - chr1_3:48316116-48316135 Msa0122010:intron 40.0%
ATAACAGATTGGGGAGGAAT+GGG - chr1_3:48316122-48316141 Msa0122010:intron 40.0%
CTCGTACTTGCTTAGTAACT+TGG + chr1_3:48316181-48316200 None:intergenic 40.0%
CAAGTTACTAAGCAAGTACG+AGG - chr1_3:48316179-48316198 Msa0122010:intron 40.0%
GGCTTATTCATTCACTCATC+AGG - chr1_3:48316227-48316246 Msa0122010:intron 40.0%
GTCTAGCTACTCTTAAGGTT+AGG - chr1_3:48316717-48316736 Msa0122010:intron 40.0%
TGAGATTTATCGCAGTGAAC+CGG - chr1_3:48316761-48316780 Msa0122010:intron 40.0%
ATAGTGTGACAATAACCCTC+AGG + chr1_3:48316949-48316968 None:intergenic 40.0%
TACAGAACACATCAGGGAAA+AGG + chr1_3:48317127-48317146 None:intergenic 40.0%
CTTCGTTACAGAACACATCA+GGG + chr1_3:48317133-48317152 None:intergenic 40.0%
ACTTCGTTACAGAACACATC+AGG + chr1_3:48317134-48317153 None:intergenic 40.0%
TTCAGATATATTTGCCGGTC+AGG + chr1_3:48317277-48317296 None:intergenic 40.0%
!!! TTCTTTTTTAGGTGGGGTAG+GGG + chr1_3:48317334-48317353 None:intergenic 40.0%
CATGAACCAAAGTAGACTAG+AGG + chr1_3:48317408-48317427 None:intergenic 40.0%
! CTCTAGTCTACTTTGGTTCA+TGG - chr1_3:48317406-48317425 Msa0122010:intron 40.0%
CTATTTGACAGAAAAACGGG+CGG - chr1_3:48317777-48317796 Msa0122010:intron 40.0%
AATTGTTCTCCTTCTCCATC+AGG + chr1_3:48317898-48317917 None:intergenic 40.0%
TCTGTAAAAAGTTCGATCCG+CGG - chr1_3:48317920-48317939 Msa0122010:intron 40.0%
!! TGATTTGCTGATTTCAGCAC+TGG + chr1_3:48317951-48317970 None:intergenic 40.0%
! CGCTGTTGGAGAATGATTTT+CGG + chr1_3:48317996-48318015 None:intergenic 40.0%
!! TTCTTGACAATTGCTGGCTT+GGG + chr1_3:48318064-48318083 None:intergenic 40.0%
! TTTCTTGACAATTGCTGGCT+TGG + chr1_3:48318065-48318084 None:intergenic 40.0%
! ACTGAATAAGGCTAGTCCAA+AGG - chr1_3:48318084-48318103 Msa0122010:CDS 40.0%
TCCAAAGGTGAGCTGTATTA+TGG - chr1_3:48318099-48318118 Msa0122010:CDS 40.0%
TCCATAATACAGCTCACCTT+TGG + chr1_3:48318103-48318122 None:intergenic 40.0%
CATTGCTGGATCAATAGTGA+GGG - chr1_3:48318180-48318199 Msa0122010:CDS 40.0%
TCCATCAGTTCCAAGTTACA+TGG - chr1_3:48318225-48318244 Msa0122010:CDS 40.0%
ACCATGTAACTTGGAACTGA+TGG + chr1_3:48318229-48318248 None:intergenic 40.0%
TTAGTTGGCACCATGTAACT+TGG + chr1_3:48318238-48318257 None:intergenic 40.0%
!! TTGGCTTTTGCAGACTTAGT+TGG + chr1_3:48318253-48318272 None:intergenic 40.0%
GAGAACACTACACCAGAAAA+AGG - chr1_3:48318301-48318320 Msa0122010:intron 40.0%
! TTTCCAAAGGACCCTTTTTC+TGG + chr1_3:48318316-48318335 None:intergenic 40.0%
CCGTTTCTTTGCATTTCCAA+AGG + chr1_3:48318329-48318348 None:intergenic 40.0%
AAAGAAACGGCTTAACTTCC+CGG - chr1_3:48318339-48318358 Msa0122010:intron 40.0%
GCAGAAATTACTGTTGGCAA+TGG - chr1_3:48318424-48318443 Msa0122010:intron 40.0%
AATTACTGTTGGCAATGGTG+TGG - chr1_3:48318429-48318448 Msa0122010:intron 40.0%
! GCAAAGTACTCCACTAGTAA+TGG - chr1_3:48318483-48318502 Msa0122010:intron 40.0%
GTAGCTTATCATTTCCTTGG+AGG - chr1_3:48318586-48318605 Msa0122010:intron 40.0%
AGTAAAGAAAACAGCCTCCA+AGG + chr1_3:48318603-48318622 None:intergenic 40.0%
TGTTCTGTGATGAGCAGTGT+GGG + chr1_3:48314521-48314540 None:intergenic 45.0%
GATCTTGGGAAGAGACAAAG+TGG + chr1_3:48314641-48314660 None:intergenic 45.0%
GGTACATCAACATCCACATC+TGG + chr1_3:48315360-48315379 None:intergenic 45.0%
TGCCAGTAAACCGAATGATG+AGG - chr1_3:48315437-48315456 Msa0122010:CDS 45.0%
ATTGCCGCAACCTCATCATT+CGG + chr1_3:48315450-48315469 None:intergenic 45.0%
!! AACCACTTTTCGGCGATACT+TGG - chr1_3:48315479-48315498 Msa0122010:CDS 45.0%
! TACCAAGTATCGCCGAAAAG+TGG + chr1_3:48315484-48315503 None:intergenic 45.0%
!! GAAGAGCATTTCGGGCTTTA+AGG - chr1_3:48315784-48315803 Msa0122010:intron 45.0%
!! AGAGCATTTCGGGCTTTAAG+GGG - chr1_3:48315786-48315805 Msa0122010:intron 45.0%
!! GAACAGGTTGAGAGCACTAA+TGG - chr1_3:48315812-48315831 Msa0122010:intron 45.0%
!! AGGTTGAGAGCACTAATGGA+AGG - chr1_3:48315816-48315835 Msa0122010:intron 45.0%
!! GGTTGAGAGCACTAATGGAA+GGG - chr1_3:48315817-48315836 Msa0122010:intron 45.0%
TAAAGTCTGCATAGAGCGGA+GGG + chr1_3:48315871-48315890 None:intergenic 45.0%
GACCTAAAGTCTGCATAGAG+CGG + chr1_3:48315875-48315894 None:intergenic 45.0%
CTAGAGATACAGGTCTCTGA+AGG - chr1_3:48315987-48316006 Msa0122010:intron 45.0%
CATAACAGATTGGGGAGGAA+TGG - chr1_3:48316121-48316140 Msa0122010:intron 45.0%
GAGATTTATCGCAGTGAACC+GGG - chr1_3:48316762-48316781 Msa0122010:intron 45.0%
CGATTCGTTATGATGAGTCC+CGG + chr1_3:48316783-48316802 None:intergenic 45.0%
CGGGACTCATCATAACGAAT+CGG - chr1_3:48316781-48316800 Msa0122010:intron 45.0%
ACTTAGGATTCATGCCGTGT+GGG - chr1_3:48316840-48316859 Msa0122010:intron 45.0%
GATACTAGACAGAACCCACA+CGG + chr1_3:48316857-48316876 None:intergenic 45.0%
! AAGAGGCATAAGCAGATGCT+AGG + chr1_3:48317075-48317094 None:intergenic 45.0%
!! AGTTTGATTTTCTCCCTGCC+TGG - chr1_3:48317096-48317115 Msa0122010:intron 45.0%
ATCAGGGAAAAGGAATCACC+AGG + chr1_3:48317117-48317136 None:intergenic 45.0%
CGTAGTACCTCTTTCTGATC+TGG - chr1_3:48317207-48317226 Msa0122010:intron 45.0%
ACTAGGTCCAGATCAGAAAG+AGG + chr1_3:48317217-48317236 None:intergenic 45.0%
GTGAGATTGCCAGATATACC+TGG - chr1_3:48317646-48317665 Msa0122010:CDS 45.0%
CCAGATATACCTGGTGAACT+CGG - chr1_3:48317655-48317674 Msa0122010:CDS 45.0%
TGGATCCAACGAATCCTAGT+TGG - chr1_3:48317831-48317850 Msa0122010:intron 45.0%
GGATCCAACGAATCCTAGTT+GGG - chr1_3:48317832-48317851 Msa0122010:intron 45.0%
GATCCAACGAATCCTAGTTG+GGG - chr1_3:48317833-48317852 Msa0122010:intron 45.0%
! AGTTAGGTGAACCCGGATTT+TGG + chr1_3:48318021-48318040 None:intergenic 45.0%
GATTGGAAGTTAGGTGAACC+CGG + chr1_3:48318028-48318047 None:intergenic 45.0%
CCTCACTATTGATCCAGCAA+TGG + chr1_3:48318182-48318201 None:intergenic 45.0%
CCATTGCTGGATCAATAGTG+AGG - chr1_3:48318179-48318198 Msa0122010:CDS 45.0%
ACACCAGAAAAAGGGTCCTT+TGG - chr1_3:48318310-48318329 Msa0122010:intron 45.0%
! AGCTGCTTTCTACCTTTGGT+GGG + chr1_3:48318399-48318418 None:intergenic 45.0%
! AAGCTGCTTTCTACCTTTGG+TGG + chr1_3:48318400-48318419 None:intergenic 45.0%
!! ATCTCGAGAGCATTCCCATT+TGG - chr1_3:48318723-48318742 Msa0122010:intron 45.0%
!! TCTCGAGAGCATTCCCATTT+GGG - chr1_3:48318724-48318743 Msa0122010:intron 45.0%
TATGTGTGTTGTGTGTGGCT+TGG - chr1_3:48318883-48318902 Msa0122010:intron 45.0%
!! TGAATAAATAAAATAAAAAA+TGG + chr1_3:48318553-48318572 None:intergenic 5.0%
CTGTTCTGTGATGAGCAGTG+TGG + chr1_3:48314522-48314541 None:intergenic 50.0%
AACTACCTAGGTGAGTGAGG+TGG + chr1_3:48314550-48314569 None:intergenic 50.0%
ACCTCACTCACCTAGGTAGT+TGG - chr1_3:48314549-48314568 Msa0122010:three_prime_UTR 50.0%
!! GGAGGTGCTTGATCAGTTTC+TGG + chr1_3:48315255-48315274 None:intergenic 50.0%
! ACCTCCTTTTCATCCACCTG+AGG - chr1_3:48315269-48315288 Msa0122010:CDS 50.0%
TCAGCCTCAGGTGGATGAAA+AGG + chr1_3:48315276-48315295 None:intergenic 50.0%
! TTTTCATCCACCTGAGGCTG+AGG - chr1_3:48315275-48315294 Msa0122010:CDS 50.0%
ATAATCTCCTCCTCAGCCTC+AGG + chr1_3:48315288-48315307 None:intergenic 50.0%
TCAAGCCGCTCCAATTGTTC+AGG - chr1_3:48315413-48315432 Msa0122010:CDS 50.0%
GGCAACCTGAACAATTGGAG+CGG + chr1_3:48315421-48315440 None:intergenic 50.0%
!! GAGCATTTCGGGCTTTAAGG+GGG - chr1_3:48315787-48315806 Msa0122010:intron 50.0%
!! CATAGAGCGGAGGGTACTAA+CGG + chr1_3:48315862-48315881 None:intergenic 50.0%
CTAAAGTCTGCATAGAGCGG+AGG + chr1_3:48315872-48315891 None:intergenic 50.0%
CTCCGCTCTATGCAGACTTT+AGG - chr1_3:48315870-48315889 Msa0122010:intron 50.0%
TGCAATCCCAGATTCGTTCG+AGG - chr1_3:48315898-48315917 Msa0122010:intron 50.0%
AATCCCAGATTCGTTCGAGG+CGG - chr1_3:48315901-48315920 Msa0122010:intron 50.0%
CACTTAGGATTCATGCCGTG+TGG - chr1_3:48316839-48316858 Msa0122010:intron 50.0%
GGAAAAGGAATCACCAGGCA+GGG + chr1_3:48317112-48317131 None:intergenic 50.0%
CATGAGTATTCCAGCCTGAC+CGG - chr1_3:48317260-48317279 Msa0122010:CDS 50.0%
GATATATTTGCCGGTCAGGC+TGG + chr1_3:48317273-48317292 None:intergenic 50.0%
CCGAGTTCACCAGGTATATC+TGG + chr1_3:48317658-48317677 None:intergenic 50.0%
GGTAAATCACCGAGTTCACC+AGG + chr1_3:48317667-48317686 None:intergenic 50.0%
CATCCCCAACTAGGATTCGT+TGG + chr1_3:48317839-48317858 None:intergenic 50.0%
CAACGAATCCTAGTTGGGGA+TGG - chr1_3:48317837-48317856 Msa0122010:intron 50.0%
GATGGAGCTGGTTAGAACGT+TGG - chr1_3:48317855-48317874 Msa0122010:intron 50.0%
GAGCTGGTTAGAACGTTGGA+TGG - chr1_3:48317859-48317878 Msa0122010:intron 50.0%
! CTTCTCCATCAGGCTTTGGT+TGG + chr1_3:48317888-48317907 None:intergenic 50.0%
CAACCAAAGCCTGATGGAGA+AGG - chr1_3:48317886-48317905 Msa0122010:intron 50.0%
TCTCCTTCTCCATCAGGCTT+TGG + chr1_3:48317892-48317911 None:intergenic 50.0%
!! TTTCAGCACTGGTAATGCCG+CGG + chr1_3:48317940-48317959 None:intergenic 50.0%
TCCAACAGCGAGCCAAAATC+CGG - chr1_3:48318006-48318025 Msa0122010:CDS 50.0%
!! TGGGAGGCTTTGAGTTGGAT+TGG + chr1_3:48318045-48318064 None:intergenic 50.0%
!! TTGACAATTGCTGGCTTGGG+AGG + chr1_3:48318061-48318080 None:intergenic 50.0%
! CTGCACACTCGTCATGCTTT+TGG + chr1_3:48318134-48318153 None:intergenic 50.0%
AAGCATGACGAGTGTGCAGT+CGG - chr1_3:48318135-48318154 Msa0122010:CDS 50.0%
AGTCTGCAAAAGCCAAGTCC+AGG - chr1_3:48318257-48318276 Msa0122010:intron 50.0%
TGGACTTTGCAACCTGGACT+TGG + chr1_3:48318272-48318291 None:intergenic 50.0%
!! AGCTGATGGACTTTGCAACC+TGG + chr1_3:48318278-48318297 None:intergenic 50.0%
GTAGTGTTCTCTGCAGCTGA+TGG + chr1_3:48318292-48318311 None:intergenic 50.0%
! TGAATGCCGCCTTGATGATC+TGG + chr1_3:48318374-48318393 None:intergenic 50.0%
AGATCATCAAGGCGGCATTC+AGG - chr1_3:48318373-48318392 Msa0122010:intron 50.0%
AACCACCACCTCACTCACCT+AGG - chr1_3:48314542-48314561 Msa0122010:three_prime_UTR 55.0%
TACCTAGGTGAGTGAGGTGG+TGG + chr1_3:48314547-48314566 None:intergenic 55.0%
GCCAACTACCTAGGTGAGTG+AGG + chr1_3:48314553-48314572 None:intergenic 55.0%
AGAAGTGGGGCCAACTACCT+AGG + chr1_3:48314562-48314581 None:intergenic 55.0%
GCCTCAGGTGGATGAAAAGG+AGG + chr1_3:48315273-48315292 None:intergenic 55.0%
AGGGCCAGTCGTAAAACGTC+AGG - chr1_3:48315836-48315855 Msa0122010:intron 55.0%
! ACGGCCTGACGTTTTACGAC+TGG + chr1_3:48315843-48315862 None:intergenic 55.0%
ATCCCAGATTCGTTCGAGGC+GGG - chr1_3:48315902-48315921 Msa0122010:intron 55.0%
GGGAAAAGGAATCACCAGGC+AGG + chr1_3:48317113-48317132 None:intergenic 55.0%
ATCCTAGTTGGGGATGGAGC+TGG - chr1_3:48317843-48317862 Msa0122010:intron 55.0%
AACCAGCTCCATCCCCAACT+AGG + chr1_3:48317848-48317867 None:intergenic 55.0%
CCAACAGCGAGCCAAAATCC+GGG - chr1_3:48318007-48318026 Msa0122010:CDS 55.0%
!! TGGCTTGGGAGGCTTTGAGT+TGG + chr1_3:48318050-48318069 None:intergenic 55.0%
CTTCGCCTGCCAGATCATCA+AGG - chr1_3:48318362-48318381 Msa0122010:intron 55.0%
TCATCCACCTGAGGCTGAGG+AGG - chr1_3:48315278-48315297 Msa0122010:CDS 60.0%
ATCTCCTCCTCAGCCTCAGG+TGG + chr1_3:48315285-48315304 None:intergenic 60.0%
!! GGGCTTTAAGGGGGCTGAAC+AGG - chr1_3:48315796-48315815 Msa0122010:intron 60.0%
GACCCGCCTCGAACGAATCT+GGG + chr1_3:48315907-48315926 None:intergenic 60.0%
TGACCCGCCTCGAACGAATC+TGG + chr1_3:48315908-48315927 None:intergenic 60.0%
AGATTCGTTCGAGGCGGGTC+AGG - chr1_3:48315907-48315926 Msa0122010:intron 60.0%
GAGGCGGGTCAGGATGTTAG+AGG - chr1_3:48315917-48315936 Msa0122010:intron 60.0%
GGCAGCCAACCAAAGCCTGA+TGG - chr1_3:48317880-48317899 Msa0122010:intron 60.0%
!!! CCCGGATTTTGGCTCGCTGT+TGG + chr1_3:48318010-48318029 None:intergenic 60.0%
GTGTGCAGTCGGAGCGTGTT+CGG - chr1_3:48318146-48318165 Msa0122010:CDS 60.0%
ATGATCTGGCAGGCGAAGCC+GGG + chr1_3:48318360-48318379 None:intergenic 60.0%
GATGATCTGGCAGGCGAAGC+CGG + chr1_3:48318361-48318380 None:intergenic 60.0%
CGCCTGCCAGATCATCAAGG+CGG - chr1_3:48318365-48318384 Msa0122010:intron 60.0%
! TGCCGCCTTGATGATCTGGC+AGG + chr1_3:48318370-48318389 None:intergenic 60.0%
TGCAGTCGGAGCGTGTTCGG+AGG - chr1_3:48318149-48318168 Msa0122010:CDS 65.0%
AGTCGGAGCGTGTTCGGAGG+AGG - chr1_3:48318152-48318171 Msa0122010:CDS 65.0%
!! CGGAGGAGGCACTCCATTGC+TGG - chr1_3:48318166-48318185 Msa0122010:CDS 65.0%
GCGGCATTCAGGCCCACCAA+AGG - chr1_3:48318384-48318403 Msa0122010:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 48314501 48318959 48314501 ID=Msa0122010;Name=Msa0122010
chr1_3 mRNA 48314501 48318959 48314501 ID=Msa0122010-mRNA-1;Parent=Msa0122010;Name=Msa0122010-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.75|0.8|0.8|1|1|5|500|445
chr1_3 exon 48314501 48315672 48314501 ID=Msa0122010-mRNA-1:exon:14574;Parent=Msa0122010-mRNA-1
chr1_3 exon 48317212 48317331 48317212 ID=Msa0122010-mRNA-1:exon:14573;Parent=Msa0122010-mRNA-1
chr1_3 exon 48317462 48317680 48317462 ID=Msa0122010-mRNA-1:exon:14572;Parent=Msa0122010-mRNA-1
chr1_3 exon 48317960 48318271 48317960 ID=Msa0122010-mRNA-1:exon:14571;Parent=Msa0122010-mRNA-1
chr1_3 exon 48318945 48318959 48318945 ID=Msa0122010-mRNA-1:exon:14570;Parent=Msa0122010-mRNA-1
chr1_3 CDS 48318945 48318959 48318945 ID=Msa0122010-mRNA-1:cds;Parent=Msa0122010-mRNA-1
chr1_3 CDS 48317960 48318271 48317960 ID=Msa0122010-mRNA-1:cds;Parent=Msa0122010-mRNA-1
chr1_3 CDS 48317462 48317680 48317462 ID=Msa0122010-mRNA-1:cds;Parent=Msa0122010-mRNA-1
chr1_3 CDS 48317212 48317331 48317212 ID=Msa0122010-mRNA-1:cds;Parent=Msa0122010-mRNA-1
chr1_3 CDS 48315001 48315672 48315001 ID=Msa0122010-mRNA-1:cds;Parent=Msa0122010-mRNA-1
chr1_3 three_prime_UTR 48314501 48315000 48314501 ID=Msa0122010-mRNA-1:three_prime_utr;Parent=Msa0122010-mRNA-1
Gene Sequence

>Msa0122010

ATGATAATTGGACTGAATTCAAGTGGATCAAAGAAGAAATGGTTTGGGAAGAAAAAATTAAACGATTCAGATTCATATCCAGAAACTGATCAAGCACCTCCTTTTCATCCACCTGAGGCTGAGGAGGAGATTATTTTAACTCATGTTGAGAATCAAAACAATCATGATCATGTTGAAGATGTACCAGATGTGGATGTTGATGTACCTGTTTCTGATGTTCAAACAGAAACTGTTGAGGTTCAAGCCGCTCCAATTGTTCAGGTTGCCAGTAAACCGAATGATGAGGTTGCGGCAATCATGATTCAAACCACTTTTCGGCGATACTTGGCAAGAAGAGCATTTCGGGCTTTAAGGGGGCTGAACAGGTTGAGAGCACTAATGGAAGGGCCAGTCGTAAAACGTCAGGCCGTTAGTACCCTCCGCTCTATGCAGACTTTAGGTCATGTGCAATCCCAGATTCGTTCGAGGCGGGTCAGGATGTTAGAGGAGACTCAAGCTCTGCAAAAACAGCTCTTACAGAAGCACACAAAAGAGCTAGAGATACAGATTGGGGAGGAATGGGATGAAAGCATACAATCAAGAGAGCAAATTGAAGCCAAGTTACTAAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATTCATTCACTCATCAGAAAAACGGGCGGAATTCATCGAAATCTATAAATCCAATGTTTATGGATCCAACGAATCCTAGTTGGGGATGGAGCTGGTTAGAACGTTGGATGGCAGCCAACCAAAGCCTGATGGAGAAGGAGAACAATTCATCTGTAAAAAGTTCGATCCGCGGCATTACCAGTGCTGAAATCAGCAAATCATTTGCTCGTTTCCAGCTCAATTCCGAAAATCATTCTCCAACAGCGAGCCAAAATCCGGGTTCACCTAACTTCCAATCCAACTCAAAGCCTCCCAAGCCAGCAATTGTCAAGAAACTGAATAAGGCTAGTCCAAAGGTGAGCTGTATTATGGATGATGACACCAAAAGCATGACGAGTGTGCAGTCGGAGCGTGTTCGGAGGAGGCACTCCATTGCTGGATCAATAGTGAGGGATGATGAAAGTCTTGCAAGCTCTCCATCAGTTCCAAGTTACATGGTGCCAACTAAGTCTGCAAAAGCCAAGTCCAGGTTGCAAAGTCCATCAGCTGCAGAGAACACTACACCAGAAAAAGGGTCCTTTGGAAATGCAAAGAAACGGCTTAACTTCCCGGCTTCGCCTGCCAGATCATCAAGGCGGCATTCAGGCCCACCAAAGGTAGAAAGCAGCTTAAATGCAGAAATTACTGTTGGCAATGGTGTGGCTGTTTGA
Protein sequence

>Msa0122010

MIIGLNSSGSKKKWFGKKKLNDSDSYPETDQAPPFHPPEAEEEIILTHVENQNNHDHVEDVPDVDVDVPVSDVQTETVEVQAAPIVQVASKPNDEVAAIMIQTTFRRYLARRAFRALRGLNRLRALMEGPVVKRQAVSTLRSMQTLGHVQSQIRSRRVRMLEETQALQKQLLQKHTKELEIQIGEEWDESIQSREQIEAKLLSKYEATMRRERAMAYSFTHQKNGRNSSKSINPMFMDPTNPSWGWSWLERWMAANQSLMEKENNSSVKSSIRGITSAEISKSFARFQLNSENHSPTASQNPGSPNFQSNSKPPKPAIVKKLNKASPKVSCIMDDDTKSMTSVQSERVRRRHSIAGSIVRDDESLASSPSVPSYMVPTKSAKAKSRLQSPSAAENTTPEKGSFGNAKKRLNFPASPARSSRRHSGPPKVESSLNAEITVGNGVAV*