AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437360 MtrunA17_Chr1g0181581 97.955 440 9 0 6 445 27 466 0.0 889
Msa1437360 MtrunA17_Chr7g0263731 70.946 444 111 9 14 444 29 467 0.0 563
Msa1437360 MtrunA17_Chr6g0462191 47.912 407 162 11 42 430 52 426 2.99e-95 293
Msa1437360 MtrunA17_Chr1g0200161 43.784 370 166 11 91 442 137 482 6.65e-79 253
Msa1437360 MtrunA17_Chr4g0045641 30.997 371 201 9 74 415 130 474 1.38e-36 140
Msa1437360 MtrunA17_Chr8g0346021 29.870 385 210 11 80 414 112 486 1.84e-35 138
Msa1437360 MtrunA17_Chr4g0025441 40.310 258 138 7 9 254 2 255 3.54e-35 135
Msa1437360 MtrunA17_Chr4g0020671 29.798 396 194 16 11 392 30 355 1.79e-29 119
Msa1437360 MtrunA17_Chr2g0283321 38.922 167 90 5 94 255 62 221 7.66e-29 115
Msa1437360 MtrunA17_Chr2g0316401 35.758 165 96 3 96 255 75 234 3.53e-27 110
Msa1437360 MtrunA17_Chr3g0135851 41.667 204 96 7 88 271 78 278 2.03e-26 110
Msa1437360 MtrunA17_Chr7g0273861 33.728 169 97 4 94 258 124 281 2.59e-24 104
Msa1437360 MtrunA17_Chr3g0126901 29.154 319 160 12 95 405 112 372 4.87e-21 95.1
Msa1437360 MtrunA17_Chr1g0146931 37.037 189 92 4 94 255 121 309 7.30e-21 94.7
Msa1437360 MtrunA17_Chr1g0204901 40.816 98 51 1 90 180 131 228 1.03e-15 79.0
Msa1437360 MtrunA17_Chr1g0203711 27.742 310 172 9 94 389 114 385 7.21e-15 76.3
Msa1437360 MtrunA17_Chr8g0389781 53.731 67 31 0 95 161 126 192 1.59e-14 75.5
Msa1437360 MtrunA17_Chr1g0172611 35.036 137 81 1 90 218 100 236 1.84e-14 74.7
Msa1437360 MtrunA17_Chr7g0273951 47.297 74 39 0 97 170 108 181 5.71e-14 73.2
Msa1437360 MtrunA17_Chr2g0330051 41.176 170 88 5 95 254 78 245 7.61e-14 73.2
Msa1437360 MtrunA17_Chr6g0472551 41.000 100 56 2 75 171 94 193 7.82e-14 73.2
Msa1437360 MtrunA17_Chr8g0340761 36.975 119 61 4 94 209 125 232 1.12e-13 72.4
Msa1437360 MtrunA17_Chr4g0060381 36.066 122 58 2 41 159 60 164 8.16e-13 70.5
Msa1437360 MtrunA17_Chr2g0330921 51.562 64 31 0 96 159 98 161 1.15e-12 68.9
Msa1437360 MtrunA17_Chr1g0166431 42.000 100 56 1 95 192 117 216 1.70e-12 68.9
Msa1437360 MtrunA17_Chr8g0342551 37.500 168 95 3 95 255 90 254 3.05e-12 68.2
Msa1437360 MtrunA17_Chr1g0156121 29.560 159 90 2 95 253 124 260 3.77e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437360 AT5G03040.1 52.045 440 172 14 24 444 41 460 2.55e-125 372
Msa1437360 AT5G03040.3 52.045 440 172 14 24 444 41 460 2.55e-125 372
Msa1437360 AT5G03040.2 52.045 440 172 14 24 444 41 460 2.55e-125 372
Msa1437360 AT3G52290.1 42.825 439 199 14 12 438 29 427 3.79e-92 285
Msa1437360 AT3G09710.1 49.497 398 162 16 60 438 76 453 7.53e-88 275
Msa1437360 AT3G09710.2 47.816 412 162 17 60 438 76 467 1.53e-84 267
Msa1437360 AT2G26410.2 34.831 356 187 8 94 440 147 466 1.82e-45 166
Msa1437360 AT2G26410.1 34.831 356 187 8 94 440 147 466 1.82e-45 166
Msa1437360 AT3G59690.2 33.025 324 166 11 98 413 172 452 6.27e-34 133
Msa1437360 AT3G59690.1 33.025 324 166 11 98 413 172 452 6.27e-34 133
Msa1437360 AT2G43680.4 30.211 331 180 10 92 415 319 605 5.18e-32 129
Msa1437360 AT2G43680.2 30.211 331 180 10 92 415 319 605 5.18e-32 129
Msa1437360 AT2G43680.3 30.211 331 180 10 92 415 319 605 5.18e-32 129
Msa1437360 AT2G43680.5 30.211 331 180 10 92 415 319 605 5.18e-32 129
Msa1437360 AT2G43680.1 30.211 331 180 10 92 415 320 606 5.45e-32 129
Msa1437360 AT3G22190.3 45.833 192 96 5 66 254 63 249 7.34e-31 123
Msa1437360 AT3G22190.2 45.833 192 96 5 66 254 63 249 7.34e-31 123
Msa1437360 AT3G22190.4 45.833 192 96 5 66 254 63 249 7.34e-31 123
Msa1437360 AT3G22190.1 45.833 192 96 5 66 254 63 249 7.34e-31 123
Msa1437360 AT2G33990.1 37.349 166 97 3 93 255 45 206 3.36e-30 118
Msa1437360 AT2G26180.1 45.665 173 80 4 94 255 83 252 1.15e-28 117
Msa1437360 AT4G00820.1 29.261 352 213 9 94 416 131 475 1.50e-28 118
Msa1437360 AT3G15050.1 38.235 170 99 4 89 255 44 210 7.95e-28 111
Msa1437360 AT1G01110.2 27.826 460 242 19 14 416 43 469 7.99e-27 113
Msa1437360 AT3G49260.3 28.616 318 169 8 94 391 119 398 2.44e-25 108
Msa1437360 AT3G49260.1 28.707 317 169 9 94 391 119 397 3.07e-25 108
Msa1437360 AT3G49260.4 28.707 317 169 9 94 391 119 397 3.07e-25 108
Msa1437360 AT3G49260.2 28.707 317 169 9 94 391 119 397 3.07e-25 108
Msa1437360 AT5G13460.1 29.605 304 138 10 96 390 112 348 4.61e-25 107
Msa1437360 AT1G72670.1 36.464 181 106 4 95 267 93 272 1.04e-21 97.1
Msa1437360 AT1G17480.1 35.714 154 87 3 95 246 94 237 5.03e-19 89.0
Msa1437360 AT4G10640.1 26.596 376 166 14 73 412 75 376 4.10e-17 83.6
Msa1437360 AT4G14750.1 44.186 86 48 0 76 161 86 171 1.16e-15 79.0
Msa1437360 AT4G14750.2 44.186 86 48 0 76 161 145 230 1.75e-15 78.6
Msa1437360 AT1G18840.1 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa1437360 AT1G18840.3 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa1437360 AT1G18840.2 42.000 100 48 1 70 159 74 173 1.85e-15 79.0
Msa1437360 AT4G14750.3 44.186 86 48 0 76 161 145 230 1.86e-15 78.6
Msa1437360 AT5G35670.1 32.124 193 110 7 70 254 132 311 1.04e-14 76.3
Msa1437360 AT1G74690.1 32.727 165 82 3 95 255 113 252 2.53e-14 75.5
Msa1437360 AT3G16490.1 50.000 62 31 0 97 158 109 170 3.79e-12 68.2
Msa1437360 AT1G51960.1 32.911 158 92 4 12 158 9 163 3.91e-11 64.7
Msa1437360 AT5G03960.3 33.333 144 64 3 92 218 107 235 6.29e-11 64.3
Msa1437360 AT5G03960.2 33.333 144 64 3 92 218 107 235 6.29e-11 64.3
Msa1437360 AT5G03960.1 33.333 144 64 3 92 218 106 234 6.32e-11 64.3
Msa1437360 AT5G07240.1 50.847 59 29 0 97 155 108 166 7.85e-11 63.9
Msa1437360 AT5G07240.2 50.847 59 29 0 97 155 154 212 9.57e-11 63.9

Find 150 sgRNAs with CRISPR-Local

Find 304 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATATTTAATTTAATTAA+TGG 0.134990 tig0028290:-7498 Msa1437360:three_prime_UTR
GAAGAGCATTTCGGGCTTTA+AGG 0.138542 tig0028290:-10493 Msa1437360:CDS
AAGAATATTTGTTGTATATC+TGG 0.154257 tig0028290:-7368 Msa1437360:three_prime_UTR
ATCTATAAATCCAATGTTTA+TGG 0.198850 tig0028290:-8471 Msa1437360:CDS
CCAGAACAGTGTAACATAAT+AGG 0.207238 tig0028290:+7435 None:intergenic
GGAGGTGCTTGATCAGTTTC+TGG 0.216889 tig0028290:+11016 None:intergenic
AACCTCATCATTCGGTTTAC+TGG 0.224722 tig0028290:+10829 None:intergenic
ACCACATTGCCCATGATAAT+TGG 0.235229 tig0028290:-11768 None:intergenic
TCAGGCAAGAAGAGCATTTC+GGG 0.235316 tig0028290:-10501 Msa1437360:intron
TGATAAGCTACACATAAATT+TGG 0.239726 tig0028290:+7708 None:intergenic
AGTTAGGTGAACCCGGATTT+TGG 0.244125 tig0028290:+8264 None:intergenic
GAGAACACTACACCAGAAAA+AGG 0.257119 tig0028290:-7981 Msa1437360:CDS
TTCAGTTTCTTGACAATTGC+TGG 0.269742 tig0028290:+8215 None:intergenic
CTGCACACTCGTCATGCTTT+TGG 0.277740 tig0028290:+8151 None:intergenic
CCTATTATGTTACACTGTTC+TGG 0.283487 tig0028290:-7435 Msa1437360:three_prime_UTR
TTCTTGACAATTGCTGGCTT+GGG 0.292014 tig0028290:+8221 None:intergenic
TCCAAAGGTGAGCTGTATTA+TGG 0.294106 tig0028290:-8183 Msa1437360:CDS
TTTGAGTTGGATTGGAAGTT+AGG 0.295442 tig0028290:+8248 None:intergenic
TTTAAGCTGCTTTCTACCTT+TGG 0.311790 tig0028290:+7882 None:intergenic
TGACCCGCCTCGAACGAATC+TGG 0.328605 tig0028290:+10372 None:intergenic
AAAGAAACGGCTTAACTTCC+CGG 0.332661 tig0028290:-7943 Msa1437360:CDS
TTTCTTGACAATTGCTGGCT+TGG 0.333380 tig0028290:+8220 None:intergenic
TCTCCTTCTCCATCAGGCTT+TGG 0.344634 tig0028290:+8393 None:intergenic
TTCAGGCAAGAAGAGCATTT+CGG 0.346402 tig0028290:-10502 Msa1437360:intron
TCAAGCCGCTCCAATTGTTC+AGG 0.350981 tig0028290:-10855 Msa1437360:CDS
CGGAGGAGGCACTCCATTGC+TGG 0.355945 tig0028290:-8116 Msa1437360:CDS
CTCGTACTTGCTTAGTAACT+TGG 0.364044 tig0028290:+10099 None:intergenic
GGCTTATTCATTCACTCATC+AGG 0.365734 tig0028290:-10050 Msa1437360:intron
CAGAACAGTGTAACATAATA+GGG 0.371935 tig0028290:+7436 None:intergenic
TGTGTAGCTTATCATTTCCT+TGG 0.374153 tig0028290:-7699 Msa1437360:three_prime_UTR
TCCAACAGCGAGCCAAAATC+CGG 0.381133 tig0028290:-8276 Msa1437360:CDS
GGATCAAAGAAGAAATGGTT+TGG 0.384554 tig0028290:-11070 Msa1437360:CDS
AATTGTTCTCCTTCTCCATC+AGG 0.385930 tig0028290:+8387 None:intergenic
TTCTGTTATCTTCATTCTTG+TGG 0.386704 tig0028290:-7528 Msa1437360:three_prime_UTR
AATTGTCAAGAAACTGAATA+AGG 0.387705 tig0028290:-8210 Msa1437360:CDS
TTTGTTGTATATCTGGGAAA+TGG 0.394441 tig0028290:-7361 Msa1437360:three_prime_UTR
CAAGTGGATCAAAGAAGAAA+TGG 0.394536 tig0028290:-11075 Msa1437360:CDS
AAGCTGCTTTCTACCTTTGG+TGG 0.401382 tig0028290:+7885 None:intergenic
CTTCTCCATCAGGCTTTGGT+TGG 0.409739 tig0028290:+8397 None:intergenic
GAACAGGTTGAGAGCACTAA+TGG 0.410177 tig0028290:-10465 Msa1437360:CDS
TGAATGCCGCCTTGATGATC+TGG 0.413022 tig0028290:+7911 None:intergenic
CTCCGCTCCATGCAGACTTT+AGG 0.414742 tig0028290:-10407 Msa1437360:CDS
TGGGAGGCTTTGAGTTGGAT+TGG 0.416717 tig0028290:+8240 None:intergenic
TTACTGGCAACCTGAACAAT+TGG 0.421900 tig0028290:+10845 None:intergenic
TGGACTTTGCAACCTGGACT+TGG 0.423630 tig0028290:+8013 None:intergenic
GATGATCTGGCAGGCGAAGC+CGG 0.424668 tig0028290:+7924 None:intergenic
GGATCCAACGAATCCTAGTT+GGG 0.426047 tig0028290:-8450 Msa1437360:CDS
TCTGTTTGAACATCAGAAAC+AGG 0.427072 tig0028290:+10890 None:intergenic
AGATCATCAAGGCGGCATTC+AGG 0.428105 tig0028290:-7909 Msa1437360:CDS
AGCTGCTTTCTACCTTTGGT+GGG 0.428437 tig0028290:+7886 None:intergenic
AAGAGCATTTCGGGCTTTAA+GGG 0.430354 tig0028290:-10492 Msa1437360:CDS
CACAAAAGAGCTAGAGATAC+AGG 0.432698 tig0028290:-10300 Msa1437360:intron
GTAGCTTATCATTTCCTTGG+AGG 0.439196 tig0028290:-7696 Msa1437360:three_prime_UTR
GAATGATAACAGTCCCAAAT+GGG 0.439513 tig0028290:+7548 None:intergenic
AGAATATTTGTTGTATATCT+GGG 0.443320 tig0028290:-7367 Msa1437360:three_prime_UTR
AGAATGATAACAGTCCCAAA+TGG 0.447334 tig0028290:+7547 None:intergenic
TTGACAATTGCTGGCTTGGG+AGG 0.449771 tig0028290:+8224 None:intergenic
TGATTTGCTGATTTCAGCAC+TGG 0.452185 tig0028290:+8334 None:intergenic
GTGTGCAGTCGGAGCGTGTT+CGG 0.453061 tig0028290:-8136 Msa1437360:CDS
TTAGTTGGCACCATGTAACT+TGG 0.454849 tig0028290:+8047 None:intergenic
AAATGAGCTGTCAGAAATCC+TGG 0.456946 tig0028290:-7582 Msa1437360:three_prime_UTR
AGATTCGTTCGAGGCGGGTC+AGG 0.459187 tig0028290:-10370 Msa1437360:CDS
TCCATAATACAGCTCACCTT+TGG 0.459798 tig0028290:+8182 None:intergenic
GATCAAAGAAGAAATGGTTT+GGG 0.464945 tig0028290:-11069 Msa1437360:CDS
TCAGCCTCAGGTGGATGAAA+AGG 0.467361 tig0028290:+10995 None:intergenic
GACCCGCCTCGAACGAATCT+GGG 0.469265 tig0028290:+10373 None:intergenic
ATTGCCGCAACCTCATCATT+CGG 0.473059 tig0028290:+10821 None:intergenic
CATAACAGATTGGGGAGGAA+TGG 0.475348 tig0028290:-10156 Msa1437360:intron
TGGATCCAACGAATCCTAGT+TGG 0.479559 tig0028290:-8451 Msa1437360:CDS
ATAACAGATTGGGGAGGAAT+GGG 0.482482 tig0028290:-10155 Msa1437360:intron
TGGGACCAGTCCAATTATCA+TGG 0.484592 tig0028290:+11758 None:intergenic
ATGATCTGGCAGGCGAAGCC+GGG 0.489921 tig0028290:+7925 None:intergenic
ATAATCTCCTCCTCAGCCTC+AGG 0.493239 tig0028290:+10983 None:intergenic
TTCGTTGGATCCATAAACAT+TGG 0.495801 tig0028290:+8461 None:intergenic
GTAGTGTTCTCTGCAGCTGA+TGG 0.498156 tig0028290:+7993 None:intergenic
AGGGCCAGTCGTAAAACGTC+AGG 0.499673 tig0028290:-10441 Msa1437360:CDS
TGGCTTGGGAGGCTTTGAGT+TGG 0.501361 tig0028290:+8235 None:intergenic
GCAAAGTACTCCACTAGTAA+TGG 0.501618 tig0028290:-7799 Msa1437360:three_prime_UTR
AGTCGGAGCGTGTTCGGAGG+AGG 0.502604 tig0028290:-8130 Msa1437360:CDS
GGTACATCAACATCCACATC+TGG 0.515322 tig0028290:+10911 None:intergenic
CATCCCCAACTAGGATTCGT+TGG 0.524622 tig0028290:+8446 None:intergenic
GAGGCGGGTCAGGATGTTAG+AGG 0.525201 tig0028290:-10360 Msa1437360:CDS
CCTTTGGAAATGCAAAGAAA+CGG 0.526599 tig0028290:-7956 Msa1437360:CDS
ATCCCAGATTCGTTCGAGGC+GGG 0.527771 tig0028290:-10375 Msa1437360:CDS
CTAGTAATGGAATTATATCA+TGG 0.529725 tig0028290:-7786 Msa1437360:three_prime_UTR
TACCAAGTATCGCCGAAAAG+TGG 0.530850 tig0028290:+10787 None:intergenic
TTAAATGCAGAAATTACTGT+TGG 0.533004 tig0028290:-7864 Msa1437360:CDS
ATCCTAGTTGGGGATGGAGC+TGG 0.536093 tig0028290:-8439 Msa1437360:CDS
TATTTGTGTTGCGTGTGGCT+TGG 0.537012 tig0028290:-7402 Msa1437360:three_prime_UTR
TATTTATTTGCTCACAAATG+TGG 0.537208 tig0028290:-7632 Msa1437360:three_prime_UTR
AGAATCACAAATAAGAAATG+AGG 0.543218 tig0028290:+7475 None:intergenic
TGCCGCCTTGATGATCTGGC+AGG 0.546459 tig0028290:+7915 None:intergenic
CTCACAAATGTGGTTCATGA+TGG 0.546881 tig0028290:-7622 Msa1437360:three_prime_UTR
AGCTGATGGACTTTGCAACC+TGG 0.547253 tig0028290:+8007 None:intergenic
TCATCCACCTGAGGCTGAGG+AGG 0.549283 tig0028290:-10990 Msa1437360:CDS
CAACCAAAGCCTGATGGAGA+AGG 0.551822 tig0028290:-8396 Msa1437360:CDS
CCGTTTCTTTGCATTTCCAA+AGG 0.555247 tig0028290:+7956 None:intergenic
GATTGGAAGTTAGGTGAACC+CGG 0.557782 tig0028290:+8257 None:intergenic
CTAAAGTCTGCATGGAGCGG+AGG 0.560515 tig0028290:+10408 None:intergenic
TCCATCAGTTCCAAGTTACA+TGG 0.565302 tig0028290:-8057 Msa1437360:CDS
TGCAATCCCAGATTCGTTCG+AGG 0.569562 tig0028290:-10379 Msa1437360:CDS
TAAACCGAATGATGAGGTTG+CGG 0.570682 tig0028290:-10825 Msa1437360:CDS
GGCAGCCAACCAAAGCCTGA+TGG 0.570992 tig0028290:-8402 Msa1437360:CDS
CACATGACCTAAAGTCTGCA+TGG 0.575607 tig0028290:+10400 None:intergenic
CAACGAATCCTAGTTGGGGA+TGG 0.576539 tig0028290:-8445 Msa1437360:CDS
ACCATGTAACTTGGAACTGA+TGG 0.577244 tig0028290:+8056 None:intergenic
AACCAGCTCCATCCCCAACT+AGG 0.580335 tig0028290:+8437 None:intergenic
AGAGCATTTCGGGCTTTAAG+GGG 0.582500 tig0028290:-10491 Msa1437360:CDS
ATCTCCTCCTCAGCCTCAGG+TGG 0.583117 tig0028290:+10986 None:intergenic
TGATATAATTCCATTACTAG+TGG 0.586145 tig0028290:+7789 None:intergenic
AGTCTGCAAAAGCCAAGTCC+AGG 0.592138 tig0028290:-8025 Msa1437360:CDS
CTTCGCCTGCCAGATCATCA+AGG 0.592606 tig0028290:-7920 Msa1437360:CDS
CCAACAGCGAGCCAAAATCC+GGG 0.594662 tig0028290:-8275 Msa1437360:CDS
TGCAGTCGGAGCGTGTTCGG+AGG 0.597571 tig0028290:-8133 Msa1437360:CDS
GAGCTGGTTAGAACGTTGGA+TGG 0.602368 tig0028290:-8423 Msa1437360:CDS
ATTGCCCATGATAATTGGAC+TGG 0.603587 tig0028290:-11763 None:intergenic
AGGTTGAGAGCACTAATGGA+AGG 0.610005 tig0028290:-10461 Msa1437360:CDS
GATGGAGCTGGTTAGAACGT+TGG 0.610918 tig0028290:-8427 Msa1437360:CDS
CATGGAGCGGAGGGTACTAA+CGG 0.611737 tig0028290:+10418 None:intergenic
AAGCATGACGAGTGTGCAGT+CGG 0.612598 tig0028290:-8147 Msa1437360:CDS
TAAAGTCTGCATGGAGCGGA+GGG 0.613022 tig0028290:+10409 None:intergenic
GCCTCAGGTGGATGAAAAGG+AGG 0.614504 tig0028290:+10998 None:intergenic
GACCTAAAGTCTGCATGGAG+CGG 0.617021 tig0028290:+10405 None:intergenic
AGCAAAGAAAACAACCTCCA+AGG 0.617429 tig0028290:+7682 None:intergenic
GGGACCAGTCCAATTATCAT+GGG 0.621023 tig0028290:+11759 None:intergenic
CCTCACTATTGATCCAGCAA+TGG 0.623634 tig0028290:+8103 None:intergenic
ACTGAATAAGGCTAGTCCAA+AGG 0.624104 tig0028290:-8198 Msa1437360:CDS
CTGTTTGATTATATAACCTG+AGG 0.625685 tig0028290:-7830 Msa1437360:exon
ATGATAACAGTCCCAAATGG+GGG 0.625936 tig0028290:+7550 None:intergenic
GAGCATTTCGGGCTTTAAGG+GGG 0.629409 tig0028290:-10490 Msa1437360:CDS
CATTGCTGGATCAATAGTGA+GGG 0.633530 tig0028290:-8102 Msa1437360:CDS
TGTTGAAGTTGTACCAGATG+TGG 0.634465 tig0028290:-10924 Msa1437360:CDS
TATGATATTTGTGTTGCGTG+TGG 0.635604 tig0028290:-7407 Msa1437360:three_prime_UTR
TGTTCAAACAGAAACTGTTG+AGG 0.641908 tig0028290:-10879 Msa1437360:CDS
GGTTGAGAGCACTAATGGAA+GGG 0.642674 tig0028290:-10460 Msa1437360:CDS
CCATTGCTGGATCAATAGTG+AGG 0.642996 tig0028290:-8103 Msa1437360:CDS
GCGGCATTCAGGCCCACCAA+AGG 0.652957 tig0028290:-7898 Msa1437360:CDS
AATGATAACAGTCCCAAATG+GGG 0.655301 tig0028290:+7549 None:intergenic
GCAGAAATTACTGTTGGCAA+TGG 0.656114 tig0028290:-7858 Msa1437360:CDS
TGATAACAGTCCCAAATGGG+GGG 0.657634 tig0028290:+7551 None:intergenic
TATGAGAAGAGAAAGAGCAA+TGG 0.661509 tig0028290:-10071 Msa1437360:CDS
AATCCCAGATTCGTTCGAGG+CGG 0.666163 tig0028290:-10376 Msa1437360:CDS
GATCCAACGAATCCTAGTTG+GGG 0.666263 tig0028290:-8449 Msa1437360:CDS
CAAGTTACTAAGCAAGTACG+AGG 0.670194 tig0028290:-10098 Msa1437360:CDS
TGCCAGTAAACCGAATGATG+AGG 0.671626 tig0028290:-10831 Msa1437360:CDS
GGCAACCTGAACAATTGGAG+CGG 0.673984 tig0028290:+10850 None:intergenic
TTTCAGCACTGGTAATGCCG+CGG 0.683035 tig0028290:+8345 None:intergenic
CGCCTGCCAGATCATCAAGG+CGG 0.686449 tig0028290:-7917 Msa1437360:CDS
ATTCTCATAACAGATTGGGG+AGG 0.696674 tig0028290:-10161 Msa1437360:intron
AATTACTGTTGGCAATGGTG+TGG 0.715956 tig0028290:-7853 Msa1437360:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGAAATTAATGCAATAATA+TGG - tig0028290:7694-7713 Msa1437360:three_prime_UTR 15.0%
!! AGAAATTAATGCAATAATAT+GGG - tig0028290:7695-7714 Msa1437360:three_prime_UTR 15.0%
!!! AAATATCAATTTTGTAATTG+AGG + tig0028290:8397-8416 None:intergenic 15.0%
!!! TATATTTGAATTTTAGCTTA+TGG - tig0028290:8893-8912 Msa1437360:intron 15.0%
!!! CATAAAAATGTCATTTTTAT+CGG + tig0028290:9405-9424 None:intergenic 15.0%
!!! TCATTTTCTATTTCTTTTTT+AGG + tig0028290:10168-10187 None:intergenic 15.0%
!! TTTAAACATATATGATTTCT+TGG + tig0028290:10397-10416 None:intergenic 15.0%
!!! TAAGTTGAATAAATTGAAGA+GGG + tig0028290:7625-7644 None:intergenic 20.0%
!! GAAATTAATGCAATAATATG+GGG - tig0028290:7696-7715 Msa1437360:three_prime_UTR 20.0%
!!! ATTTTGTTTTGATATCATTC+AGG - tig0028290:8586-8605 Msa1437360:intron 20.0%
!!! TTTTATTCTGAAGTATATCA+GGG + tig0028290:8849-8868 None:intergenic 20.0%
!!! ATTTTATTCTGAAGTATATC+AGG + tig0028290:8850-8869 None:intergenic 20.0%
!!! AAATTTTTGCATATAAAGCA+TGG - tig0028290:9292-9311 Msa1437360:intron 20.0%
!! TATGAAAAAAATAAGTGTCA+TGG - tig0028290:9421-9440 Msa1437360:intron 20.0%
!!! TTAAATCTTGAGTTATTTTG+AGG + tig0028290:9467-9486 None:intergenic 20.0%
!! AATAAAAACAACTTAAAGAG+AGG + tig0028290:9817-9836 None:intergenic 20.0%
!!! TTTCTATTTCTTTTTTAGGT+GGG + tig0028290:10164-10183 None:intergenic 20.0%
!!! TTTTCTATTTCTTTTTTAGG+TGG + tig0028290:10165-10184 None:intergenic 20.0%
!! AAATTATGTTAGCAGAAAAT+AGG + tig0028290:10511-10530 None:intergenic 20.0%
!!! TAGAAGTGTATATTTATCTT+AGG + tig0028290:10583-10602 None:intergenic 20.0%
!! ATCTATAAATCCAATGTTTA+TGG - tig0028290:10634-10653 Msa1437360:intron 20.0%
!!! AAGAATATTTGTTGTATATC+TGG - tig0028290:11737-11756 Msa1437360:intron 20.0%
!!! AGAATATTTGTTGTATATCT+GGG - tig0028290:11738-11757 Msa1437360:intron 20.0%
! ATGTGATTAGATTAGATCTT+GGG + tig0028290:7507-7526 None:intergenic 25.0%
! AATGTGATTAGATTAGATCT+TGG + tig0028290:7508-7527 None:intergenic 25.0%
! AAATGAAGAAAAAAGAAGAG+AGG + tig0028290:7549-7568 None:intergenic 25.0%
!! GTAAGTTGAATAAATTGAAG+AGG + tig0028290:7626-7645 None:intergenic 25.0%
! TTCACATTAGAAAACCAATT+TGG + tig0028290:7731-7750 None:intergenic 25.0%
!! GTCAAATTGTTTTCATACAA+CGG + tig0028290:7886-7905 None:intergenic 25.0%
! TATGTTTAATCTCAATTACC+TGG - tig0028290:7968-7987 Msa1437360:CDS 25.0%
! ATGTTTAATCTCAATTACCT+GGG - tig0028290:7969-7988 Msa1437360:CDS 25.0%
! ATTCAACACATAATAGTAAG+AGG + tig0028290:8353-8372 None:intergenic 25.0%
! TAAAAGATGTGTATGTTACA+TGG - tig0028290:8454-8473 Msa1437360:CDS 25.0%
! AACAAAATTCAGCTTGAAAA+TGG + tig0028290:8574-8593 None:intergenic 25.0%
!!! GAATTTTAGCTTATGGATTA+TGG - tig0028290:8900-8919 Msa1437360:intron 25.0%
!!! TAAGAAGTTGCATTTGTTTT+AGG - tig0028290:9092-9111 Msa1437360:intron 25.0%
! TTTACACATAGTATAAAGCA+TGG - tig0028290:9210-9229 Msa1437360:intron 25.0%
!! TTTTTGAACTGAAAGATCTA+AGG - tig0028290:9241-9260 Msa1437360:intron 25.0%
! TTTCATGTAATTTACCTTAC+TGG + tig0028290:9275-9294 None:intergenic 25.0%
! ACAAGATTTGACTAACAATT+CGG + tig0028290:9361-9380 None:intergenic 25.0%
! TTGTTAGTCAAATCTTGTTA+TGG - tig0028290:9363-9382 Msa1437360:intron 25.0%
!!! CATTTTGATATTTCATAGCA+TGG + tig0028290:9744-9763 None:intergenic 25.0%
!! GTAGTTTCAGATATATTTTC+CGG + tig0028290:10105-10124 None:intergenic 25.0%
!!! TTCTATTTCTTTTTTAGGTG+GGG + tig0028290:10163-10182 None:intergenic 25.0%
! CACATCTGATATAAAAATTG+TGG + tig0028290:10200-10219 None:intergenic 25.0%
!! AATTTTGTAGATGAAAACTC+AGG + tig0028290:10373-10392 None:intergenic 25.0%
! TATTTATCTTAGGTCAAACT+TGG + tig0028290:10573-10592 None:intergenic 25.0%
! ACTTCTATTTGACAGAAAAA+CGG - tig0028290:10596-10615 Msa1437360:intron 25.0%
! AATTGTCAAGAAACTGAATA+AGG - tig0028290:10895-10914 Msa1437360:CDS 25.0%
! TTAAATGCAGAAATTACTGT+TGG - tig0028290:11241-11260 Msa1437360:intron 25.0%
! TGATATAATTCCATTACTAG+TGG + tig0028290:11319-11338 None:intergenic 25.0%
!! CTAGTAATGGAATTATATCA+TGG - tig0028290:11319-11338 Msa1437360:intron 25.0%
! TGATAAGCTACACATAAATT+TGG + tig0028290:11400-11419 None:intergenic 25.0%
! TATTTATTTGCTCACAAATG+TGG - tig0028290:11473-11492 Msa1437360:intron 25.0%
! AGAATCACAAATAAGAAATG+AGG + tig0028290:11633-11652 None:intergenic 25.0%
!!! TGTTTGTTTTGTTTAGAGTC+AGG + tig0028290:7767-7786 None:intergenic 30.0%
CTCTAAACAAAACAAACATC+AGG - tig0028290:7769-7788 Msa1437360:three_prime_UTR 30.0%
! CTGATTTTGTAGAATTCAAG+TGG - tig0028290:8014-8033 Msa1437360:CDS 30.0%
GATCAAAGAAGAAATGGTTT+GGG - tig0028290:8036-8055 Msa1437360:CDS 30.0%
!! TCATGATTCAAACCACTTTT+CGG - tig0028290:8306-8325 Msa1437360:CDS 30.0%
! CATACACATCTTTTATCGTA+CGG + tig0028290:8449-8468 None:intergenic 30.0%
TCCAAATTCTCATAACAGAT+TGG - tig0028290:8939-8958 Msa1437360:intron 30.0%
CCAAATTCTCATAACAGATT+GGG - tig0028290:8940-8959 Msa1437360:intron 30.0%
CAAATTCTCATAACAGATTG+GGG - tig0028290:8941-8960 Msa1437360:intron 30.0%
ATGCAACTTCTTAAGAGAAA+TGG + tig0028290:9085-9104 None:intergenic 30.0%
! AAATGAGAAAAGAGCATGTA+TGG - tig0028290:9129-9148 Msa1437360:intron 30.0%
TTGATGAACTGAAAGATCAA+AGG - tig0028290:9156-9175 Msa1437360:intron 30.0%
CTGAAAGATCAAAGGAAAAA+TGG - tig0028290:9164-9183 Msa1437360:intron 30.0%
TATATCTTCTGATTCACGAT+CGG + tig0028290:9334-9353 None:intergenic 30.0%
GAAAAGTAGTTAGCTATATG+AGG - tig0028290:9508-9527 Msa1437360:intron 30.0%
AAAATGTCTAGCTACTCTTA+AGG - tig0028290:9540-9559 Msa1437360:intron 30.0%
TAACGAATCGGAATTTAATG+TGG - tig0028290:9621-9640 Msa1437360:intron 30.0%
AACGAATCGGAATTTAATGT+GGG - tig0028290:9622-9641 Msa1437360:intron 30.0%
TCCAAAATAGATACACACTT+AGG - tig0028290:9652-9671 Msa1437360:intron 30.0%
! TCCTAAGTGTGTATCTATTT+TGG + tig0028290:9656-9675 None:intergenic 30.0%
TTCTGTATCAAATCGAGATA+CGG - tig0028290:9691-9710 Msa1437360:intron 30.0%
ATATCAAAATGAGAATCCTG+AGG - tig0028290:9753-9772 Msa1437360:intron 30.0%
TATCAAAATGAGAATCCTGA+GGG - tig0028290:9754-9773 Msa1437360:intron 30.0%
AAAAACAACTTAAAGAGAGG+AGG + tig0028290:9814-9833 None:intergenic 30.0%
ACACACAAGCTTAATAACTT+TGG - tig0028290:9844-9863 Msa1437360:intron 30.0%
CACACAAGCTTAATAACTTT+GGG - tig0028290:9845-9864 Msa1437360:intron 30.0%
!! CTTTTGAGAATTACAGAAAG+TGG - tig0028290:9988-10007 Msa1437360:intron 30.0%
TCATGATTCATTAGCAAAAC+TGG + tig0028290:10068-10087 None:intergenic 30.0%
CTTACAAAATTAATCGACGT+GGG + tig0028290:10345-10364 None:intergenic 30.0%
AAAGATCAAGAAACTCAAAC+TGG + tig0028290:10535-10554 None:intergenic 30.0%
CTTCTATTTGACAGAAAAAC+GGG - tig0028290:10597-10616 Msa1437360:intron 30.0%
CTGTTTGATTATATAACCTG+AGG - tig0028290:11275-11294 Msa1437360:intron 30.0%
TTCTGTTATCTTCATTCTTG+TGG - tig0028290:11577-11596 Msa1437360:intron 30.0%
CAGAACAGTGTAACATAATA+GGG + tig0028290:11672-11691 None:intergenic 30.0%
! TTTGTTGTATATCTGGGAAA+TGG - tig0028290:11744-11763 Msa1437360:intron 30.0%
AAGAAGAGAGAGAAAGAAGT+GGG + tig0028290:7428-7447 None:intergenic 35.0%
AAAGAAGAGAGAGAAAGAAG+TGG + tig0028290:7429-7448 None:intergenic 35.0%
GAGAGGTTGAAGATAAAAGA+GGG + tig0028290:7532-7551 None:intergenic 35.0%
AGAGAGGTTGAAGATAAAAG+AGG + tig0028290:7533-7552 None:intergenic 35.0%
TCTCTCTTGCATTTCACTTT+AGG - tig0028290:7587-7606 Msa1437360:three_prime_UTR 35.0%
TAGTTTATACATACGCACTG+AGG - tig0028290:7931-7950 Msa1437360:CDS 35.0%
AACACATTAAGAAACTTCCC+AGG + tig0028290:7989-8008 None:intergenic 35.0%
CAAGTGGATCAAAGAAGAAA+TGG - tig0028290:8030-8049 Msa1437360:CDS 35.0%
GGATCAAAGAAGAAATGGTT+TGG - tig0028290:8035-8054 Msa1437360:CDS 35.0%
TCTGTTTGAACATCAGAAAC+AGG + tig0028290:8218-8237 None:intergenic 35.0%
TGTTCAAACAGAAACTGTTG+AGG - tig0028290:8226-8245 Msa1437360:CDS 35.0%
ATAACTTGGCATCGTTAAAG+AGG + tig0028290:8491-8510 None:intergenic 35.0%
TAATCGACAATTGCTGCAAA+AGG - tig0028290:8525-8544 Msa1437360:intron 35.0%
CCCAATCTGTTATGAGAATT+TGG + tig0028290:8943-8962 None:intergenic 35.0%
TATGAGAAGAGAAAGAGCAA+TGG - tig0028290:9034-9053 Msa1437360:intron 35.0%
!!! GCATTTGTTTTAGGTTAGTC+AGG - tig0028290:9101-9120 Msa1437360:intron 35.0%
CAAAGGAAAAATGGCTAGTA+AGG - tig0028290:9173-9192 Msa1437360:intron 35.0%
CTAAGGAAAAATGACCAGTA+AGG - tig0028290:9258-9277 Msa1437360:intron 35.0%
GGGAGAAAATCAAACTTAAG+AGG + tig0028290:9915-9934 None:intergenic 35.0%
! TAGCAAAACTGGTTCATACT+AGG + tig0028290:10057-10076 None:intergenic 35.0%
! TTCAGATATATTTTCCGGTC+AGG + tig0028290:10100-10119 None:intergenic 35.0%
!!! TTTCTTTTTTAGGTGGGGTA+GGG + tig0028290:10158-10177 None:intergenic 35.0%
!!! ATTTCTTTTTTAGGTGGGGT+AGG + tig0028290:10159-10178 None:intergenic 35.0%
ACAAATCCTCTAGTCTACTT+TGG - tig0028290:10222-10241 Msa1437360:intron 35.0%
GCTTACAAAATTAATCGACG+TGG + tig0028290:10346-10365 None:intergenic 35.0%
TTCGTTGGATCCATAAACAT+TGG + tig0028290:10647-10666 None:intergenic 35.0%
! AATGATTTTCGGAATTGAGC+TGG + tig0028290:10808-10827 None:intergenic 35.0%
!! TTTGAGTTGGATTGGAAGTT+AGG + tig0028290:10860-10879 None:intergenic 35.0%
TTCAGTTTCTTGACAATTGC+TGG + tig0028290:10893-10912 None:intergenic 35.0%
AGAACACTACACCAGAAAAA+GGG - tig0028290:11125-11144 Msa1437360:intron 35.0%
! CCTTTGGAAATGCAAAGAAA+CGG - tig0028290:11149-11168 Msa1437360:intron 35.0%
! TTTAAGCTGCTTTCTACCTT+TGG + tig0028290:11226-11245 None:intergenic 35.0%
! TACTTTGCATTCTTTTCCTC+AGG + tig0028290:11294-11313 None:intergenic 35.0%
TGTGTAGCTTATCATTTCCT+TGG - tig0028290:11406-11425 Msa1437360:intron 35.0%
AATGATAACAGTCCCAAATG+GGG + tig0028290:11559-11578 None:intergenic 35.0%
GAATGATAACAGTCCCAAAT+GGG + tig0028290:11560-11579 None:intergenic 35.0%
AGAATGATAACAGTCCCAAA+TGG + tig0028290:11561-11580 None:intergenic 35.0%
CCAGAACAGTGTAACATAAT+AGG + tig0028290:11673-11692 None:intergenic 35.0%
CCTATTATGTTACACTGTTC+TGG - tig0028290:11670-11689 Msa1437360:intron 35.0%
TATGATATTTGTGTTGCGTG+TGG - tig0028290:11698-11717 Msa1437360:intron 35.0%
AGAAGAGAGAGAAAGAAGTG+GGG + tig0028290:7427-7446 None:intergenic 40.0%
TGGGGAAGAATAAGCCAAAT+TGG - tig0028290:7714-7733 Msa1437360:three_prime_UTR 40.0%
TCAAACATTCTCACAAGCGT+CGG + tig0028290:7832-7851 None:intergenic 40.0%
TGTTGAAGTTGTACCAGATG+TGG - tig0028290:8181-8200 Msa1437360:CDS 40.0%
TTACTGGCAACCTGAACAAT+TGG + tig0028290:8263-8282 None:intergenic 40.0%
AACCTCATCATTCGGTTTAC+TGG + tig0028290:8279-8298 None:intergenic 40.0%
TAAACCGAATGATGAGGTTG+CGG - tig0028290:8280-8299 Msa1437360:CDS 40.0%
CCACTAATGACAGCATAACT+TGG + tig0028290:8505-8524 None:intergenic 40.0%
CCAAGTTATGCTGTCATTAG+TGG - tig0028290:8502-8521 Msa1437360:intron 40.0%
!! TTCAGGCAAGAAGAGCATTT+CGG - tig0028290:8603-8622 Msa1437360:intron 40.0%
!! AAGAGCATTTCGGGCTTTAA+GGG - tig0028290:8613-8632 Msa1437360:intron 40.0%
CACAAAAGAGCTAGAGATAC+AGG - tig0028290:8805-8824 Msa1437360:intron 40.0%
TAGAGATACAGGTCTCTGAA+GGG - tig0028290:8816-8835 Msa1437360:intron 40.0%
ATTCTCATAACAGATTGGGG+AGG - tig0028290:8944-8963 Msa1437360:intron 40.0%
ATAACAGATTGGGGAGGAAT+GGG - tig0028290:8950-8969 Msa1437360:intron 40.0%
CTCGTACTTGCTTAGTAACT+TGG + tig0028290:9009-9028 None:intergenic 40.0%
CAAGTTACTAAGCAAGTACG+AGG - tig0028290:9007-9026 Msa1437360:intron 40.0%
GGCTTATTCATTCACTCATC+AGG - tig0028290:9055-9074 Msa1437360:intron 40.0%
GTCTAGCTACTCTTAAGGTT+AGG - tig0028290:9545-9564 Msa1437360:intron 40.0%
TGAGATTTATCGCAGTGAAC+CGG - tig0028290:9589-9608 Msa1437360:intron 40.0%
GATTTGATACAGAACCCACA+CGG + tig0028290:9685-9704 None:intergenic 40.0%
ATAGTGTGACAATAACCCTC+AGG + tig0028290:9772-9791 None:intergenic 40.0%
TACAGAACACATCAGGGAAA+AGG + tig0028290:9950-9969 None:intergenic 40.0%
CTTCGTTACAGAACACATCA+GGG + tig0028290:9956-9975 None:intergenic 40.0%
ACTTCGTTACAGAACACATC+AGG + tig0028290:9957-9976 None:intergenic 40.0%
!!! TTCTTTTTTAGGTGGGGTAG+GGG + tig0028290:10157-10176 None:intergenic 40.0%
CATGAACCAAAGTAGACTAG+AGG + tig0028290:10231-10250 None:intergenic 40.0%
! CTCTAGTCTACTTTGGTTCA+TGG - tig0028290:10229-10248 Msa1437360:intron 40.0%
CTATTTGACAGAAAAACGGG+CGG - tig0028290:10600-10619 Msa1437360:intron 40.0%
AATTGTTCTCCTTCTCCATC+AGG + tig0028290:10721-10740 None:intergenic 40.0%
TCTGTAAAAAGTTCGATCCG+CGG - tig0028290:10743-10762 Msa1437360:intron 40.0%
!! TGATTTGCTGATTTCAGCAC+TGG + tig0028290:10774-10793 None:intergenic 40.0%
! CGCTGTTGGAGAATGATTTT+CGG + tig0028290:10819-10838 None:intergenic 40.0%
!! TTCTTGACAATTGCTGGCTT+GGG + tig0028290:10887-10906 None:intergenic 40.0%
! TTTCTTGACAATTGCTGGCT+TGG + tig0028290:10888-10907 None:intergenic 40.0%
! ACTGAATAAGGCTAGTCCAA+AGG - tig0028290:10907-10926 Msa1437360:CDS 40.0%
TCCAAAGGTGAGCTGTATTA+TGG - tig0028290:10922-10941 Msa1437360:CDS 40.0%
TCCATAATACAGCTCACCTT+TGG + tig0028290:10926-10945 None:intergenic 40.0%
CATTGCTGGATCAATAGTGA+GGG - tig0028290:11003-11022 Msa1437360:CDS 40.0%
TCCATCAGTTCCAAGTTACA+TGG - tig0028290:11048-11067 Msa1437360:CDS 40.0%
ACCATGTAACTTGGAACTGA+TGG + tig0028290:11052-11071 None:intergenic 40.0%
TTAGTTGGCACCATGTAACT+TGG + tig0028290:11061-11080 None:intergenic 40.0%
!! TTGGCTTTTGCAGACTTAGT+TGG + tig0028290:11076-11095 None:intergenic 40.0%
GAGAACACTACACCAGAAAA+AGG - tig0028290:11124-11143 Msa1437360:intron 40.0%
! TTTCCAAAGGACCCTTTTTC+TGG + tig0028290:11139-11158 None:intergenic 40.0%
CCGTTTCTTTGCATTTCCAA+AGG + tig0028290:11152-11171 None:intergenic 40.0%
AAAGAAACGGCTTAACTTCC+CGG - tig0028290:11162-11181 Msa1437360:intron 40.0%
GCAGAAATTACTGTTGGCAA+TGG - tig0028290:11247-11266 Msa1437360:intron 40.0%
AATTACTGTTGGCAATGGTG+TGG - tig0028290:11252-11271 Msa1437360:intron 40.0%
! GCAAAGTACTCCACTAGTAA+TGG - tig0028290:11306-11325 Msa1437360:intron 40.0%
GTAGCTTATCATTTCCTTGG+AGG - tig0028290:11409-11428 Msa1437360:intron 40.0%
AGCAAAGAAAACAACCTCCA+AGG + tig0028290:11426-11445 None:intergenic 40.0%
CTCACAAATGTGGTTCATGA+TGG - tig0028290:11483-11502 Msa1437360:intron 40.0%
AAATGAGCTGTCAGAAATCC+TGG - tig0028290:11523-11542 Msa1437360:intron 40.0%
ATGATAACAGTCCCAAATGG+GGG + tig0028290:11558-11577 None:intergenic 40.0%
TGTTCTGTGATGAGCAGTGT+GGG + tig0028290:7369-7388 None:intergenic 45.0%
GATCTTGGGAAGAGACAAAG+TGG + tig0028290:7493-7512 None:intergenic 45.0%
GGTACATCAACATCCACATC+TGG + tig0028290:8197-8216 None:intergenic 45.0%
TGCCAGTAAACCGAATGATG+AGG - tig0028290:8274-8293 Msa1437360:CDS 45.0%
ATTGCCGCAACCTCATCATT+CGG + tig0028290:8287-8306 None:intergenic 45.0%
!! AACCACTTTTCGGCGATACT+TGG - tig0028290:8316-8335 Msa1437360:CDS 45.0%
! TACCAAGTATCGCCGAAAAG+TGG + tig0028290:8321-8340 None:intergenic 45.0%
!! TCAGGCAAGAAGAGCATTTC+GGG - tig0028290:8604-8623 Msa1437360:intron 45.0%
!! GAAGAGCATTTCGGGCTTTA+AGG - tig0028290:8612-8631 Msa1437360:intron 45.0%
!! AGAGCATTTCGGGCTTTAAG+GGG - tig0028290:8614-8633 Msa1437360:intron 45.0%
!! GAACAGGTTGAGAGCACTAA+TGG - tig0028290:8640-8659 Msa1437360:intron 45.0%
!! AGGTTGAGAGCACTAATGGA+AGG - tig0028290:8644-8663 Msa1437360:intron 45.0%
!! GGTTGAGAGCACTAATGGAA+GGG - tig0028290:8645-8664 Msa1437360:intron 45.0%
CACATGACCTAAAGTCTGCA+TGG + tig0028290:8708-8727 None:intergenic 45.0%
CTAGAGATACAGGTCTCTGA+AGG - tig0028290:8815-8834 Msa1437360:intron 45.0%
CATAACAGATTGGGGAGGAA+TGG - tig0028290:8949-8968 Msa1437360:intron 45.0%
GAGATTTATCGCAGTGAACC+GGG - tig0028290:9590-9609 Msa1437360:intron 45.0%
CGATTCGTTATGATGAGTCC+CGG + tig0028290:9611-9630 None:intergenic 45.0%
CGGGACTCATCATAACGAAT+CGG - tig0028290:9609-9628 Msa1437360:intron 45.0%
ACTTAGGATTCATGCCGTGT+GGG - tig0028290:9668-9687 Msa1437360:intron 45.0%
! AAGAGGCATAAGCAGATGCT+AGG + tig0028290:9898-9917 None:intergenic 45.0%
!! AGTTTGATTTTCTCCCTGCC+TGG - tig0028290:9919-9938 Msa1437360:intron 45.0%
ATCAGGGAAAAGGAATCACC+AGG + tig0028290:9940-9959 None:intergenic 45.0%
CGTAGTACCTCTTTCTGATC+TGG - tig0028290:10030-10049 Msa1437360:intron 45.0%
ACTAGGTCCAGATCAGAAAG+AGG + tig0028290:10040-10059 None:intergenic 45.0%
! GATATATTTTCCGGTCAGGC+TGG + tig0028290:10096-10115 None:intergenic 45.0%
GTGAGATTGCCAGATATACC+TGG - tig0028290:10469-10488 Msa1437360:CDS 45.0%
CCAGATATACCTGGTGAACT+CGG - tig0028290:10478-10497 Msa1437360:CDS 45.0%
TGGATCCAACGAATCCTAGT+TGG - tig0028290:10654-10673 Msa1437360:intron 45.0%
GGATCCAACGAATCCTAGTT+GGG - tig0028290:10655-10674 Msa1437360:intron 45.0%
GATCCAACGAATCCTAGTTG+GGG - tig0028290:10656-10675 Msa1437360:intron 45.0%
! AGTTAGGTGAACCCGGATTT+TGG + tig0028290:10844-10863 None:intergenic 45.0%
GATTGGAAGTTAGGTGAACC+CGG + tig0028290:10851-10870 None:intergenic 45.0%
CCTCACTATTGATCCAGCAA+TGG + tig0028290:11005-11024 None:intergenic 45.0%
CCATTGCTGGATCAATAGTG+AGG - tig0028290:11002-11021 Msa1437360:CDS 45.0%
ACACCAGAAAAAGGGTCCTT+TGG - tig0028290:11133-11152 Msa1437360:intron 45.0%
! AGCTGCTTTCTACCTTTGGT+GGG + tig0028290:11222-11241 None:intergenic 45.0%
! AAGCTGCTTTCTACCTTTGG+TGG + tig0028290:11223-11242 None:intergenic 45.0%
TGATAACAGTCCCAAATGGG+GGG + tig0028290:11557-11576 None:intergenic 45.0%
TATTTGTGTTGCGTGTGGCT+TGG - tig0028290:11703-11722 Msa1437360:intron 45.0%
!! TGAATAAATAAAATAAAAAA+TGG + tig0028290:11376-11395 None:intergenic 5.0%
!!! TGTATATTTAATTTAATTAA+TGG - tig0028290:11607-11626 Msa1437360:intron 5.0%
CTGTTCTGTGATGAGCAGTG+TGG + tig0028290:7370-7389 None:intergenic 50.0%
ACTACCTAGGTGAGTGAGTG+AGG + tig0028290:7401-7420 None:intergenic 50.0%
CACTCACTCACCTAGGTAGT+TGG - tig0028290:7401-7420 Msa1437360:three_prime_UTR 50.0%
!! GGAGGTGCTTGATCAGTTTC+TGG + tig0028290:8092-8111 None:intergenic 50.0%
! ACCTCCTTTTCATCCACCTG+AGG - tig0028290:8106-8125 Msa1437360:CDS 50.0%
TCAGCCTCAGGTGGATGAAA+AGG + tig0028290:8113-8132 None:intergenic 50.0%
! TTTTCATCCACCTGAGGCTG+AGG - tig0028290:8112-8131 Msa1437360:CDS 50.0%
ATAATCTCCTCCTCAGCCTC+AGG + tig0028290:8125-8144 None:intergenic 50.0%
TCAAGCCGCTCCAATTGTTC+AGG - tig0028290:8250-8269 Msa1437360:CDS 50.0%
GGCAACCTGAACAATTGGAG+CGG + tig0028290:8258-8277 None:intergenic 50.0%
!! GAGCATTTCGGGCTTTAAGG+GGG - tig0028290:8615-8634 Msa1437360:intron 50.0%
TAAAGTCTGCATGGAGCGGA+GGG + tig0028290:8699-8718 None:intergenic 50.0%
GACCTAAAGTCTGCATGGAG+CGG + tig0028290:8703-8722 None:intergenic 50.0%
TGCAATCCCAGATTCGTTCG+AGG - tig0028290:8726-8745 Msa1437360:intron 50.0%
AATCCCAGATTCGTTCGAGG+CGG - tig0028290:8729-8748 Msa1437360:intron 50.0%
CACTTAGGATTCATGCCGTG+TGG - tig0028290:9667-9686 Msa1437360:intron 50.0%
GGAAAAGGAATCACCAGGCA+GGG + tig0028290:9935-9954 None:intergenic 50.0%
CATGAGTATTCCAGCCTGAC+CGG - tig0028290:10083-10102 Msa1437360:CDS 50.0%
CCGAGTTCACCAGGTATATC+TGG + tig0028290:10481-10500 None:intergenic 50.0%
GGTAAATCACCGAGTTCACC+AGG + tig0028290:10490-10509 None:intergenic 50.0%
CATCCCCAACTAGGATTCGT+TGG + tig0028290:10662-10681 None:intergenic 50.0%
CAACGAATCCTAGTTGGGGA+TGG - tig0028290:10660-10679 Msa1437360:intron 50.0%
GATGGAGCTGGTTAGAACGT+TGG - tig0028290:10678-10697 Msa1437360:intron 50.0%
GAGCTGGTTAGAACGTTGGA+TGG - tig0028290:10682-10701 Msa1437360:intron 50.0%
! CTTCTCCATCAGGCTTTGGT+TGG + tig0028290:10711-10730 None:intergenic 50.0%
CAACCAAAGCCTGATGGAGA+AGG - tig0028290:10709-10728 Msa1437360:intron 50.0%
TCTCCTTCTCCATCAGGCTT+TGG + tig0028290:10715-10734 None:intergenic 50.0%
!! TTTCAGCACTGGTAATGCCG+CGG + tig0028290:10763-10782 None:intergenic 50.0%
TCCAACAGCGAGCCAAAATC+CGG - tig0028290:10829-10848 Msa1437360:CDS 50.0%
!! TGGGAGGCTTTGAGTTGGAT+TGG + tig0028290:10868-10887 None:intergenic 50.0%
!! TTGACAATTGCTGGCTTGGG+AGG + tig0028290:10884-10903 None:intergenic 50.0%
! CTGCACACTCGTCATGCTTT+TGG + tig0028290:10957-10976 None:intergenic 50.0%
AAGCATGACGAGTGTGCAGT+CGG - tig0028290:10958-10977 Msa1437360:CDS 50.0%
AGTCTGCAAAAGCCAAGTCC+AGG - tig0028290:11080-11099 Msa1437360:CDS 50.0%
TGGACTTTGCAACCTGGACT+TGG + tig0028290:11095-11114 None:intergenic 50.0%
!! AGCTGATGGACTTTGCAACC+TGG + tig0028290:11101-11120 None:intergenic 50.0%
GTAGTGTTCTCTGCAGCTGA+TGG + tig0028290:11115-11134 None:intergenic 50.0%
! TGAATGCCGCCTTGATGATC+TGG + tig0028290:11197-11216 None:intergenic 50.0%
AGATCATCAAGGCGGCATTC+AGG - tig0028290:11196-11215 Msa1437360:intron 50.0%
TAGGTGAGTGAGTGAGGTGG+TGG + tig0028290:7395-7414 None:intergenic 55.0%
ACCACCTCACTCACTCACCT+AGG - tig0028290:7394-7413 Msa1437360:three_prime_UTR 55.0%
ACCTAGGTGAGTGAGTGAGG+TGG + tig0028290:7398-7417 None:intergenic 55.0%
AGAAGTGGGGCCAACTACCT+AGG + tig0028290:7414-7433 None:intergenic 55.0%
GCCTCAGGTGGATGAAAAGG+AGG + tig0028290:8110-8129 None:intergenic 55.0%
AGGGCCAGTCGTAAAACGTC+AGG - tig0028290:8664-8683 Msa1437360:intron 55.0%
! ACGGCCTGACGTTTTACGAC+TGG + tig0028290:8671-8690 None:intergenic 55.0%
!! CATGGAGCGGAGGGTACTAA+CGG + tig0028290:8690-8709 None:intergenic 55.0%
CTAAAGTCTGCATGGAGCGG+AGG + tig0028290:8700-8719 None:intergenic 55.0%
CTCCGCTCCATGCAGACTTT+AGG - tig0028290:8698-8717 Msa1437360:intron 55.0%
ATCCCAGATTCGTTCGAGGC+GGG - tig0028290:8730-8749 Msa1437360:intron 55.0%
GGGAAAAGGAATCACCAGGC+AGG + tig0028290:9936-9955 None:intergenic 55.0%
ATCCTAGTTGGGGATGGAGC+TGG - tig0028290:10666-10685 Msa1437360:intron 55.0%
AACCAGCTCCATCCCCAACT+AGG + tig0028290:10671-10690 None:intergenic 55.0%
CCAACAGCGAGCCAAAATCC+GGG - tig0028290:10830-10849 Msa1437360:CDS 55.0%
!! TGGCTTGGGAGGCTTTGAGT+TGG + tig0028290:10873-10892 None:intergenic 55.0%
CTTCGCCTGCCAGATCATCA+AGG - tig0028290:11185-11204 Msa1437360:intron 55.0%
AAATGGGGGGAATGCTCTCC+AGG + tig0028290:11544-11563 None:intergenic 55.0%
!! TGGAGAGCATTCCCCCCATT+TGG - tig0028290:11543-11562 Msa1437360:intron 55.0%
!! GGAGAGCATTCCCCCCATTT+GGG - tig0028290:11544-11563 Msa1437360:intron 55.0%
TCATCCACCTGAGGCTGAGG+AGG - tig0028290:8115-8134 Msa1437360:CDS 60.0%
ATCTCCTCCTCAGCCTCAGG+TGG + tig0028290:8122-8141 None:intergenic 60.0%
!! GGGCTTTAAGGGGGCTGAAC+AGG - tig0028290:8624-8643 Msa1437360:intron 60.0%
GACCCGCCTCGAACGAATCT+GGG + tig0028290:8735-8754 None:intergenic 60.0%
TGACCCGCCTCGAACGAATC+TGG + tig0028290:8736-8755 None:intergenic 60.0%
AGATTCGTTCGAGGCGGGTC+AGG - tig0028290:8735-8754 Msa1437360:intron 60.0%
GAGGCGGGTCAGGATGTTAG+AGG - tig0028290:8745-8764 Msa1437360:intron 60.0%
GGCAGCCAACCAAAGCCTGA+TGG - tig0028290:10703-10722 Msa1437360:intron 60.0%
!!! CCCGGATTTTGGCTCGCTGT+TGG + tig0028290:10833-10852 None:intergenic 60.0%
GTGTGCAGTCGGAGCGTGTT+CGG - tig0028290:10969-10988 Msa1437360:CDS 60.0%
ATGATCTGGCAGGCGAAGCC+GGG + tig0028290:11183-11202 None:intergenic 60.0%
GATGATCTGGCAGGCGAAGC+CGG + tig0028290:11184-11203 None:intergenic 60.0%
CGCCTGCCAGATCATCAAGG+CGG - tig0028290:11188-11207 Msa1437360:intron 60.0%
! TGCCGCCTTGATGATCTGGC+AGG + tig0028290:11193-11212 None:intergenic 60.0%
TGCAGTCGGAGCGTGTTCGG+AGG - tig0028290:10972-10991 Msa1437360:CDS 65.0%
AGTCGGAGCGTGTTCGGAGG+AGG - tig0028290:10975-10994 Msa1437360:CDS 65.0%
!! CGGAGGAGGCACTCCATTGC+TGG - tig0028290:10989-11008 Msa1437360:CDS 65.0%
GCGGCATTCAGGCCCACCAA+AGG - tig0028290:11207-11226 Msa1437360:intron 65.0%
Chromosome Type Strat End Strand Name
tig0028290 gene 7349 11778 7349 ID=Msa1437360;Name=Msa1437360
tig0028290 mRNA 7349 11778 7349 ID=Msa1437360-mRNA-1;Parent=Msa1437360;Name=Msa1437360-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0.75|0.8|0.8|1|1|5|496|445
tig0028290 exon 7349 8516 7349 ID=Msa1437360-mRNA-1:exon:10647;Parent=Msa1437360-mRNA-1
tig0028290 exon 10051 10170 10051 ID=Msa1437360-mRNA-1:exon:10646;Parent=Msa1437360-mRNA-1
tig0028290 exon 10301 10519 10301 ID=Msa1437360-mRNA-1:exon:10645;Parent=Msa1437360-mRNA-1
tig0028290 exon 10790 11101 10790 ID=Msa1437360-mRNA-1:exon:10644;Parent=Msa1437360-mRNA-1
tig0028290 exon 11764 11778 11764 ID=Msa1437360-mRNA-1:exon:10643;Parent=Msa1437360-mRNA-1
tig0028290 CDS 11764 11778 11764 ID=Msa1437360-mRNA-1:cds;Parent=Msa1437360-mRNA-1
tig0028290 CDS 10790 11101 10790 ID=Msa1437360-mRNA-1:cds;Parent=Msa1437360-mRNA-1
tig0028290 CDS 10301 10519 10301 ID=Msa1437360-mRNA-1:cds;Parent=Msa1437360-mRNA-1
tig0028290 CDS 10051 10170 10051 ID=Msa1437360-mRNA-1:cds;Parent=Msa1437360-mRNA-1
tig0028290 CDS 7845 8516 7845 ID=Msa1437360-mRNA-1:cds;Parent=Msa1437360-mRNA-1
tig0028290 three_prime_UTR 7349 7844 7349 ID=Msa1437360-mRNA-1:three_prime_utr;Parent=Msa1437360-mRNA-1
Gene Sequence

>Msa1437360

ATGATAATTGGACTGAATTCAAGTGGATCAAAGAAGAAATGGTTTGGGAAGAAAAAATTAAACGATTCAGATTCATATCCAGAAACTGATCAAGCACCTCCTTTTCATCCACCTGAGGCTGAGGAGGAGATTATTTTAACTCATGTTGAGAATCAAAACAATCATGATCATGTTGAAGTTGTACCAGATGTGGATGTTGATGTACCTGTTTCTGATGTTCAAACAGAAACTGTTGAGGTTCAAGCCGCTCCAATTGTTCAGGTTGCCAGTAAACCGAATGATGAGGTTGCGGCAATCATGATTCAAACCACTTTTCGGCGATACTTGGCAAGAAGAGCATTTCGGGCTTTAAGGGGGCTGAACAGGTTGAGAGCACTAATGGAAGGGCCAGTCGTAAAACGTCAGGCCGTTAGTACCCTCCGCTCCATGCAGACTTTAGGTCATGTGCAATCCCAGATTCGTTCGAGGCGGGTCAGGATGTTAGAGGAGACTCAAGCTCTGCAAAAACAGCTCTTACAGAAGCACACAAAAGAGCTAGAGATACAGATTGGGGAGGAATGGGATGAAAGCATACAATCAAGAGAGCAAATTGAAGCCAAGTTACTAAGCAAGTACGAGGCTACTATGAGAAGAGAAAGAGCAATGGCTTATTCATTCACTCATCAGAAAAACGGGCGGAATTCATCGAAATCTATAAATCCAATGTTTATGGATCCAACGAATCCTAGTTGGGGATGGAGCTGGTTAGAACGTTGGATGGCAGCCAACCAAAGCCTGATGGAGAAGGAGAACAATTCATCTGTAAAAAGTTCGATCCGCGGCATTACCAGTGCTGAAATCAGCAAATCATTTGCTCGTTTCCAGCTCAATTCCGAAAATCATTCTCCAACAGCGAGCCAAAATCCGGGTTCACCTAACTTCCAATCCAACTCAAAGCCTCCCAAGCCAGCAATTGTCAAGAAACTGAATAAGGCTAGTCCAAAGGTGAGCTGTATTATGGATGATGACACCAAAAGCATGACGAGTGTGCAGTCGGAGCGTGTTCGGAGGAGGCACTCCATTGCTGGATCAATAGTGAGGGATGATGAAAGTCTTGCAAGCTCTCCATCAGTTCCAAGTTACATGGTGCCAACTAAGTCTGCAAAAGCCAAGTCCAGGTTGCAAAGTCCATCAGCTGCAGAGAACACTACACCAGAAAAAGGGTCCTTTGGAAATGCAAAGAAACGGCTTAACTTCCCGGCTTCGCCTGCCAGATCATCAAGGCGGCATTCAGGCCCACCAAAGGTAGAAAGCAGCTTAAATGCAGAAATTACTGTTGGCAATGGTGTGGCTGTTTGA
Protein sequence

>Msa1437360

MIIGLNSSGSKKKWFGKKKLNDSDSYPETDQAPPFHPPEAEEEIILTHVENQNNHDHVEVVPDVDVDVPVSDVQTETVEVQAAPIVQVASKPNDEVAAIMIQTTFRRYLARRAFRALRGLNRLRALMEGPVVKRQAVSTLRSMQTLGHVQSQIRSRRVRMLEETQALQKQLLQKHTKELEIQIGEEWDESIQSREQIEAKLLSKYEATMRRERAMAYSFTHQKNGRNSSKSINPMFMDPTNPSWGWSWLERWMAANQSLMEKENNSSVKSSIRGITSAEISKSFARFQLNSENHSPTASQNPGSPNFQSNSKPPKPAIVKKLNKASPKVSCIMDDDTKSMTSVQSERVRRRHSIAGSIVRDDESLASSPSVPSYMVPTKSAKAKSRLQSPSAAENTTPEKGSFGNAKKRLNFPASPARSSRRHSGPPKVESSLNAEITVGNGVAV*