AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127290 MtrunA17_Chr1g0190661 93.056 144 10 0 1 144 1 144 3.14e-100 283
Msa0127290 MtrunA17_Chr1g0190671 91.667 144 12 0 1 144 1 144 8.56e-98 277
Msa0127290 MtrunA17_Chr1g0190651 86.806 144 19 0 1 144 1 144 1.75e-91 261
Msa0127290 MtrunA17_Chr7g0274181 72.174 115 32 0 30 144 70 184 7.09e-61 186
Msa0127290 MtrunA17_Chr1g0190641 59.854 137 54 1 8 144 4 139 1.34e-58 178
Msa0127290 MtrunA17_Chr6g0484021 48.026 152 59 4 10 144 31 179 4.60e-43 149
Msa0127290 MtrunA17_Chr6g0483971 48.175 137 64 3 10 144 11 142 1.01e-39 135
Msa0127290 MtrunA17_Chr6g0484041 45.333 150 62 5 10 144 23 167 2.88e-35 128
Msa0127290 MtrunA17_Chr6g0484031 42.254 142 68 3 10 144 11 145 3.94e-34 124
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127290 AT3G04720.1 71.074 121 35 0 24 144 71 191 2.20e-62 191

Find 50 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGGAATAATAGGGGATTT+TGG 0.183068 1_3:+56541166 Msa0127290:three_prime_UTR
ACTTGCTATTGTGGAATAAT+AGG 0.188926 1_3:+56541157 Msa0127290:three_prime_UTR
ACAACCCTCAGAATATTAAT+TGG 0.199861 1_3:+56540217 Msa0127290:CDS
CTTGCTATTGTGGAATAATA+GGG 0.358615 1_3:+56541158 Msa0127290:three_prime_UTR
CAAGGGCTGGTTTGCATCCC+AGG 0.360799 1_3:-56540271 None:intergenic
ACAGAGAACTTTGAGCTTGT+TGG 0.365729 1_3:+56540119 Msa0127290:CDS
CAAAGACATAGTTAACTATA+AGG 0.374893 1_3:-56540988 None:intergenic
TAACTATAAGGTGACCCTTC+TGG 0.391434 1_3:-56540976 None:intergenic
CTCTCTACCACGAGGGCCCT+GGG 0.401142 1_3:-56540334 None:intergenic
AGAAGTAAATTCGATGTTAT+AGG 0.404130 1_3:+56541191 Msa0127290:three_prime_UTR
CAACCCTCAGAATATTAATT+GGG 0.408460 1_3:+56540218 Msa0127290:CDS
CAGGTGACAAATACTGCAAC+TGG 0.420771 1_3:+56540854 Msa0127290:intron
ACTCTCTACCACGAGGGCCC+TGG 0.449243 1_3:-56540335 None:intergenic
AGGGCCCTGGGGTCCACAAA+AGG 0.466880 1_3:-56540322 None:intergenic
TACTATATAACTTGCTATTG+TGG 0.469574 1_3:+56541148 Msa0127290:three_prime_UTR
GTCAGAGTGCAAACGGTGTA+AGG 0.476091 1_3:+56540178 Msa0127290:CDS
CCAGTGTATACTGTGCTACC+TGG 0.477513 1_3:+56540253 Msa0127290:CDS
TCAAATTGATACCAATGGAC+AGG 0.479120 1_3:+56540949 Msa0127290:CDS
TAATATTCTGAGGGTTGTAA+AGG 0.485495 1_3:-56540212 None:intergenic
CTGGGATGCAAACCAGCCCT+TGG 0.489658 1_3:+56540272 Msa0127290:CDS
CGCCAATGGTGGATTAGACT+TGG 0.495624 1_3:+56540910 Msa0127290:CDS
CTAATCCACCATTGGCGCAC+TGG 0.495852 1_3:-56540904 None:intergenic
GTCTTGTGGCAAGTGCTTGA+GGG 0.497781 1_3:+56540356 Msa0127290:CDS
AGCAGTGCTACACGTATACT+TGG 0.514144 1_3:+56541092 Msa0127290:three_prime_UTR
AGTCTTGTGGCAAGTGCTTG+AGG 0.518693 1_3:+56540355 Msa0127290:CDS
TAATCCCAATTAATATTCTG+AGG 0.523387 1_3:-56540222 None:intergenic
TTCAATCAAATTGATACCAA+TGG 0.541175 1_3:+56540944 Msa0127290:CDS
CTTCTGGTAACCCTGTCCAT+TGG 0.545362 1_3:-56540960 None:intergenic
GTGAATGGTCAGAGTGCAAA+CGG 0.554003 1_3:+56540171 Msa0127290:CDS
TATTCTGAGGGTTGTAAAGG+TGG 0.557673 1_3:-56540209 None:intergenic
ATCCAAGTCTAATCCACCAT+TGG 0.563000 1_3:-56540912 None:intergenic
AATGGACAGGGTTACCAGAA+GGG 0.563174 1_3:+56540962 Msa0127290:CDS
ATAGTGGACCAGTGCGCCAA+TGG 0.565572 1_3:+56540896 Msa0127290:CDS
ATGCAAACCAGCCCTTGGAA+TGG 0.572438 1_3:+56540277 Msa0127290:CDS
AATCCCAATTAATATTCTGA+GGG 0.576550 1_3:-56540221 None:intergenic
GTGGACCAGTGCGCCAATGG+TGG 0.584793 1_3:+56540899 Msa0127290:CDS
CAAATTGATACCAATGGACA+GGG 0.591064 1_3:+56540950 Msa0127290:CDS
TTGCTATTGTGGAATAATAG+GGG 0.594706 1_3:+56541159 Msa0127290:three_prime_UTR
TCAAGTAACAGTGAGAATAG+TGG 0.598835 1_3:+56540880 Msa0127290:CDS
CAGTGTATACTGTGCTACCT+GGG 0.602353 1_3:+56540254 Msa0127290:CDS
GTTGGAACGATGTTAGTGAA+TGG 0.611199 1_3:+56540156 Msa0127290:CDS
TGTGTTTCATAGTAGAAGCA+TGG 0.611414 1_3:-56540072 None:intergenic
CAATGGACAGGGTTACCAGA+AGG 0.617429 1_3:+56540961 Msa0127290:CDS
TGTGGACCCCAGGGCCCTCG+TGG 0.633299 1_3:+56540327 Msa0127290:CDS
TCACTAACATCGTTCCAACA+AGG 0.650356 1_3:-56540152 None:intergenic
TCTCTACCACGAGGGCCCTG+GGG 0.658114 1_3:-56540333 None:intergenic
CCAGGTAGCACAGTATACAC+TGG 0.672657 1_3:-56540253 None:intergenic
CCTCGTGGTAGAGAGTCTTG+TGG 0.684944 1_3:+56540342 Msa0127290:CDS
CACAAGACTCTCTACCACGA+GGG 0.694332 1_3:-56540341 None:intergenic
CCACAAGACTCTCTACCACG+AGG 0.710327 1_3:-56540342 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATAACACATTTATAATT+AGG + chr1_3:56540627-56540646 Msa0127290:intron 10.0%
!!! GTTTTATTTATTGTATTATT+AGG - chr1_3:56541071-56541090 None:intergenic 10.0%
!! TTTATAACACATTTATAATT+AGG + chr1_3:56540627-56540646 Msa0127290:intron 10.0%
!!! GTTTTATTTATTGTATTATT+AGG - chr1_3:56541071-56541090 None:intergenic 10.0%
!!! TTTTATAAAATGAGTAAGTA+CGG - chr1_3:56540387-56540406 None:intergenic 15.0%
!!! TTTTATAAAATGAGTAAGTA+CGG - chr1_3:56540387-56540406 None:intergenic 15.0%
!! ACCTAAACACATTTATAATT+AGG + chr1_3:56540550-56540569 Msa0127290:intron 20.0%
!! ACCTAATTATAAATGTGTTT+AGG - chr1_3:56540554-56540573 None:intergenic 20.0%
!! GATAGTTGAATTAATATTAG+TGG + chr1_3:56540823-56540842 Msa0127290:intron 20.0%
!! ACCTAAACACATTTATAATT+AGG + chr1_3:56540550-56540569 Msa0127290:intron 20.0%
!! ACCTAATTATAAATGTGTTT+AGG - chr1_3:56540554-56540573 None:intergenic 20.0%
!! GATAGTTGAATTAATATTAG+TGG + chr1_3:56540823-56540842 Msa0127290:intron 20.0%
! AACACACAAAAAAGAGAAAA+TGG + chr1_3:56540089-56540108 Msa0127290:exon 25.0%
! AATCCCAATTAATATTCTGA+GGG - chr1_3:56540224-56540243 None:intergenic 25.0%
! TAATCCCAATTAATATTCTG+AGG - chr1_3:56540225-56540244 None:intergenic 25.0%
!! ATTAGGTCATAAATCGTTTT+CGG + chr1_3:56540567-56540586 Msa0127290:intron 25.0%
!!! ATCTCTATGAGATTTTTTGT+CGG + chr1_3:56540739-56540758 Msa0127290:intron 25.0%
! TTCAATCAAATTGATACCAA+TGG + chr1_3:56540944-56540963 Msa0127290:CDS 25.0%
! CAAAGACATAGTTAACTATA+AGG - chr1_3:56540991-56541010 None:intergenic 25.0%
!!! TACTATATAACTTGCTATTG+TGG + chr1_3:56541148-56541167 Msa0127290:three_prime_UTR 25.0%
! AGAAGTAAATTCGATGTTAT+AGG + chr1_3:56541191-56541210 Msa0127290:three_prime_UTR 25.0%
!! CTTAATTTTAAGCGTTTTGT+CGG + chr1_3:56541243-56541262 Msa0127290:three_prime_UTR 25.0%
! AACACACAAAAAAGAGAAAA+TGG + chr1_3:56540089-56540108 Msa0127290:exon 25.0%
! AATCCCAATTAATATTCTGA+GGG - chr1_3:56540224-56540243 None:intergenic 25.0%
! TAATCCCAATTAATATTCTG+AGG - chr1_3:56540225-56540244 None:intergenic 25.0%
!! ATTAGGTCATAAATCGTTTT+CGG + chr1_3:56540567-56540586 Msa0127290:intron 25.0%
!!! ATCTCTATGAGATTTTTTGT+CGG + chr1_3:56540739-56540758 Msa0127290:intron 25.0%
! TTCAATCAAATTGATACCAA+TGG + chr1_3:56540944-56540963 Msa0127290:CDS 25.0%
! CAAAGACATAGTTAACTATA+AGG - chr1_3:56540991-56541010 None:intergenic 25.0%
!!! TACTATATAACTTGCTATTG+TGG + chr1_3:56541148-56541167 Msa0127290:three_prime_UTR 25.0%
! AGAAGTAAATTCGATGTTAT+AGG + chr1_3:56541191-56541210 Msa0127290:three_prime_UTR 25.0%
!! CTTAATTTTAAGCGTTTTGT+CGG + chr1_3:56541243-56541262 Msa0127290:three_prime_UTR 25.0%
! TAATATTCTGAGGGTTGTAA+AGG - chr1_3:56540215-56540234 None:intergenic 30.0%
ACAACCCTCAGAATATTAAT+TGG + chr1_3:56540217-56540236 Msa0127290:CDS 30.0%
CAACCCTCAGAATATTAATT+GGG + chr1_3:56540218-56540237 Msa0127290:CDS 30.0%
CTATGAACTCTATAATAGCT+AGG + chr1_3:56540597-56540616 Msa0127290:intron 30.0%
! ATTAGGTCATAAACCGTTTT+CGG + chr1_3:56540644-56540663 Msa0127290:intron 30.0%
!!! TCTCTATGAGATTTTTTGTC+GGG + chr1_3:56540740-56540759 Msa0127290:intron 30.0%
!! ATTCAACTCAGTTTTAGTCA+AGG + chr1_3:56540778-56540797 Msa0127290:intron 30.0%
! AATATTAGTGGTGATGATAC+AGG + chr1_3:56540835-56540854 Msa0127290:intron 30.0%
!! ACTTGCTATTGTGGAATAAT+AGG + chr1_3:56541157-56541176 Msa0127290:three_prime_UTR 30.0%
!! CTTGCTATTGTGGAATAATA+GGG + chr1_3:56541158-56541177 Msa0127290:three_prime_UTR 30.0%
! TTGCTATTGTGGAATAATAG+GGG + chr1_3:56541159-56541178 Msa0127290:three_prime_UTR 30.0%
! TAATATTCTGAGGGTTGTAA+AGG - chr1_3:56540215-56540234 None:intergenic 30.0%
ACAACCCTCAGAATATTAAT+TGG + chr1_3:56540217-56540236 Msa0127290:CDS 30.0%
CAACCCTCAGAATATTAATT+GGG + chr1_3:56540218-56540237 Msa0127290:CDS 30.0%
CTATGAACTCTATAATAGCT+AGG + chr1_3:56540597-56540616 Msa0127290:intron 30.0%
! ATTAGGTCATAAACCGTTTT+CGG + chr1_3:56540644-56540663 Msa0127290:intron 30.0%
!!! TCTCTATGAGATTTTTTGTC+GGG + chr1_3:56540740-56540759 Msa0127290:intron 30.0%
!! ATTCAACTCAGTTTTAGTCA+AGG + chr1_3:56540778-56540797 Msa0127290:intron 30.0%
! AATATTAGTGGTGATGATAC+AGG + chr1_3:56540835-56540854 Msa0127290:intron 30.0%
!! ACTTGCTATTGTGGAATAAT+AGG + chr1_3:56541157-56541176 Msa0127290:three_prime_UTR 30.0%
!! CTTGCTATTGTGGAATAATA+GGG + chr1_3:56541158-56541177 Msa0127290:three_prime_UTR 30.0%
! TTGCTATTGTGGAATAATAG+GGG + chr1_3:56541159-56541178 Msa0127290:three_prime_UTR 30.0%
TGTGTTTCATAGTAGAAGCA+TGG - chr1_3:56540075-56540094 None:intergenic 35.0%
!!! TTGGTTTTGTGTTTCCTTGT+TGG + chr1_3:56540138-56540157 Msa0127290:CDS 35.0%
!! TACTTTTTACGCCATTCCAA+GGG - chr1_3:56540291-56540310 None:intergenic 35.0%
!! ATACTTTTTACGCCATTCCA+AGG - chr1_3:56540292-56540311 None:intergenic 35.0%
TTGGAATGGCGTAAAAAGTA+TGG + chr1_3:56540291-56540310 Msa0127290:CDS 35.0%
! AATGGCGTAAAAAGTATGGT+TGG + chr1_3:56540295-56540314 Msa0127290:CDS 35.0%
TCATAGAAACAAGCCGAAAA+CGG - chr1_3:56540583-56540602 None:intergenic 35.0%
!!! CTCTATGAGATTTTTTGTCG+GGG + chr1_3:56540741-56540760 Msa0127290:intron 35.0%
!!! TCTATGAGATTTTTTGTCGG+GGG + chr1_3:56540742-56540761 Msa0127290:intron 35.0%
TCAAGTAACAGTGAGAATAG+TGG + chr1_3:56540880-56540899 Msa0127290:CDS 35.0%
TCAAATTGATACCAATGGAC+AGG + chr1_3:56540949-56540968 Msa0127290:CDS 35.0%
CAAATTGATACCAATGGACA+GGG + chr1_3:56540950-56540969 Msa0127290:CDS 35.0%
! TGTGGAATAATAGGGGATTT+TGG + chr1_3:56541166-56541185 Msa0127290:three_prime_UTR 35.0%
TGTGTTTCATAGTAGAAGCA+TGG - chr1_3:56540075-56540094 None:intergenic 35.0%
!!! TTGGTTTTGTGTTTCCTTGT+TGG + chr1_3:56540138-56540157 Msa0127290:CDS 35.0%
!! TACTTTTTACGCCATTCCAA+GGG - chr1_3:56540291-56540310 None:intergenic 35.0%
!! ATACTTTTTACGCCATTCCA+AGG - chr1_3:56540292-56540311 None:intergenic 35.0%
TTGGAATGGCGTAAAAAGTA+TGG + chr1_3:56540291-56540310 Msa0127290:CDS 35.0%
! AATGGCGTAAAAAGTATGGT+TGG + chr1_3:56540295-56540314 Msa0127290:CDS 35.0%
TCATAGAAACAAGCCGAAAA+CGG - chr1_3:56540583-56540602 None:intergenic 35.0%
!!! CTCTATGAGATTTTTTGTCG+GGG + chr1_3:56540741-56540760 Msa0127290:intron 35.0%
!!! TCTATGAGATTTTTTGTCGG+GGG + chr1_3:56540742-56540761 Msa0127290:intron 35.0%
TCAAGTAACAGTGAGAATAG+TGG + chr1_3:56540880-56540899 Msa0127290:CDS 35.0%
TCAAATTGATACCAATGGAC+AGG + chr1_3:56540949-56540968 Msa0127290:CDS 35.0%
CAAATTGATACCAATGGACA+GGG + chr1_3:56540950-56540969 Msa0127290:CDS 35.0%
! TGTGGAATAATAGGGGATTT+TGG + chr1_3:56541166-56541185 Msa0127290:three_prime_UTR 35.0%
ACAGAGAACTTTGAGCTTGT+TGG + chr1_3:56540119-56540138 Msa0127290:CDS 40.0%
TCACTAACATCGTTCCAACA+AGG - chr1_3:56540155-56540174 None:intergenic 40.0%
GTTGGAACGATGTTAGTGAA+TGG + chr1_3:56540156-56540175 Msa0127290:CDS 40.0%
! TATTCTGAGGGTTGTAAAGG+TGG - chr1_3:56540212-56540231 None:intergenic 40.0%
ATCCAAGTCTAATCCACCAT+TGG - chr1_3:56540915-56540934 None:intergenic 40.0%
TAACTATAAGGTGACCCTTC+TGG - chr1_3:56540979-56540998 None:intergenic 40.0%
ACAGAGAACTTTGAGCTTGT+TGG + chr1_3:56540119-56540138 Msa0127290:CDS 40.0%
TCACTAACATCGTTCCAACA+AGG - chr1_3:56540155-56540174 None:intergenic 40.0%
GTTGGAACGATGTTAGTGAA+TGG + chr1_3:56540156-56540175 Msa0127290:CDS 40.0%
! TATTCTGAGGGTTGTAAAGG+TGG - chr1_3:56540212-56540231 None:intergenic 40.0%
ATCCAAGTCTAATCCACCAT+TGG - chr1_3:56540915-56540934 None:intergenic 40.0%
TAACTATAAGGTGACCCTTC+TGG - chr1_3:56540979-56540998 None:intergenic 40.0%
GTGAATGGTCAGAGTGCAAA+CGG + chr1_3:56540171-56540190 Msa0127290:CDS 45.0%
CAGTGTATACTGTGCTACCT+GGG + chr1_3:56540254-56540273 Msa0127290:CDS 45.0%
! TATGGTTGGACTGCCTTTTG+TGG + chr1_3:56540309-56540328 Msa0127290:CDS 45.0%
CAGGTGACAAATACTGCAAC+TGG + chr1_3:56540854-56540873 Msa0127290:intron 45.0%
AATGGACAGGGTTACCAGAA+GGG + chr1_3:56540962-56540981 Msa0127290:CDS 45.0%
AGCAGTGCTACACGTATACT+TGG + chr1_3:56541092-56541111 Msa0127290:three_prime_UTR 45.0%
GTGAATGGTCAGAGTGCAAA+CGG + chr1_3:56540171-56540190 Msa0127290:CDS 45.0%
CAGTGTATACTGTGCTACCT+GGG + chr1_3:56540254-56540273 Msa0127290:CDS 45.0%
! TATGGTTGGACTGCCTTTTG+TGG + chr1_3:56540309-56540328 Msa0127290:CDS 45.0%
CAGGTGACAAATACTGCAAC+TGG + chr1_3:56540854-56540873 Msa0127290:intron 45.0%
AATGGACAGGGTTACCAGAA+GGG + chr1_3:56540962-56540981 Msa0127290:CDS 45.0%
AGCAGTGCTACACGTATACT+TGG + chr1_3:56541092-56541111 Msa0127290:three_prime_UTR 45.0%
GTCAGAGTGCAAACGGTGTA+AGG + chr1_3:56540178-56540197 Msa0127290:CDS 50.0%
CCAGGTAGCACAGTATACAC+TGG - chr1_3:56540256-56540275 None:intergenic 50.0%
CCAGTGTATACTGTGCTACC+TGG + chr1_3:56540253-56540272 Msa0127290:CDS 50.0%
ATGCAAACCAGCCCTTGGAA+TGG + chr1_3:56540277-56540296 Msa0127290:CDS 50.0%
! TTTTACGCCATTCCAAGGGC+TGG - chr1_3:56540287-56540306 None:intergenic 50.0%
CACAAGACTCTCTACCACGA+GGG - chr1_3:56540344-56540363 None:intergenic 50.0%
! AGTCTTGTGGCAAGTGCTTG+AGG + chr1_3:56540355-56540374 Msa0127290:CDS 50.0%
GTCTTGTGGCAAGTGCTTGA+GGG + chr1_3:56540356-56540375 Msa0127290:CDS 50.0%
!! CGCCAATGGTGGATTAGACT+TGG + chr1_3:56540910-56540929 Msa0127290:CDS 50.0%
CTTCTGGTAACCCTGTCCAT+TGG - chr1_3:56540963-56540982 None:intergenic 50.0%
CAATGGACAGGGTTACCAGA+AGG + chr1_3:56540961-56540980 Msa0127290:CDS 50.0%
GTCAGAGTGCAAACGGTGTA+AGG + chr1_3:56540178-56540197 Msa0127290:CDS 50.0%
CCAGGTAGCACAGTATACAC+TGG - chr1_3:56540256-56540275 None:intergenic 50.0%
CCAGTGTATACTGTGCTACC+TGG + chr1_3:56540253-56540272 Msa0127290:CDS 50.0%
ATGCAAACCAGCCCTTGGAA+TGG + chr1_3:56540277-56540296 Msa0127290:CDS 50.0%
! TTTTACGCCATTCCAAGGGC+TGG - chr1_3:56540287-56540306 None:intergenic 50.0%
CACAAGACTCTCTACCACGA+GGG - chr1_3:56540344-56540363 None:intergenic 50.0%
! AGTCTTGTGGCAAGTGCTTG+AGG + chr1_3:56540355-56540374 Msa0127290:CDS 50.0%
GTCTTGTGGCAAGTGCTTGA+GGG + chr1_3:56540356-56540375 Msa0127290:CDS 50.0%
!! CGCCAATGGTGGATTAGACT+TGG + chr1_3:56540910-56540929 Msa0127290:CDS 50.0%
CTTCTGGTAACCCTGTCCAT+TGG - chr1_3:56540963-56540982 None:intergenic 50.0%
CAATGGACAGGGTTACCAGA+AGG + chr1_3:56540961-56540980 Msa0127290:CDS 50.0%
! ACTGCCTTTTGTGGACCCCA+GGG + chr1_3:56540318-56540337 Msa0127290:CDS 55.0%
CCACAAGACTCTCTACCACG+AGG - chr1_3:56540345-56540364 None:intergenic 55.0%
CCTCGTGGTAGAGAGTCTTG+TGG + chr1_3:56540342-56540361 Msa0127290:CDS 55.0%
!! ATAGTGGACCAGTGCGCCAA+TGG + chr1_3:56540896-56540915 Msa0127290:CDS 55.0%
CTAATCCACCATTGGCGCAC+TGG - chr1_3:56540907-56540926 None:intergenic 55.0%
! ACTGCCTTTTGTGGACCCCA+GGG + chr1_3:56540318-56540337 Msa0127290:CDS 55.0%
CCACAAGACTCTCTACCACG+AGG - chr1_3:56540345-56540364 None:intergenic 55.0%
CCTCGTGGTAGAGAGTCTTG+TGG + chr1_3:56540342-56540361 Msa0127290:CDS 55.0%
!! ATAGTGGACCAGTGCGCCAA+TGG + chr1_3:56540896-56540915 Msa0127290:CDS 55.0%
CTAATCCACCATTGGCGCAC+TGG - chr1_3:56540907-56540926 None:intergenic 55.0%
!! CAAGGGCTGGTTTGCATCCC+AGG - chr1_3:56540274-56540293 None:intergenic 60.0%
CTGGGATGCAAACCAGCCCT+TGG + chr1_3:56540272-56540291 Msa0127290:CDS 60.0%
! GACTGCCTTTTGTGGACCCC+AGG + chr1_3:56540317-56540336 Msa0127290:CDS 60.0%
!! CAAGGGCTGGTTTGCATCCC+AGG - chr1_3:56540274-56540293 None:intergenic 60.0%
CTGGGATGCAAACCAGCCCT+TGG + chr1_3:56540272-56540291 Msa0127290:CDS 60.0%
! GACTGCCTTTTGTGGACCCC+AGG + chr1_3:56540317-56540336 Msa0127290:CDS 60.0%
AGGGCCCTGGGGTCCACAAA+AGG - chr1_3:56540325-56540344 None:intergenic 65.0%
TCTCTACCACGAGGGCCCTG+GGG - chr1_3:56540336-56540355 None:intergenic 65.0%
CTCTCTACCACGAGGGCCCT+GGG - chr1_3:56540337-56540356 None:intergenic 65.0%
ACTCTCTACCACGAGGGCCC+TGG - chr1_3:56540338-56540357 None:intergenic 65.0%
GTGGACCAGTGCGCCAATGG+TGG + chr1_3:56540899-56540918 Msa0127290:CDS 65.0%
AGGGCCCTGGGGTCCACAAA+AGG - chr1_3:56540325-56540344 None:intergenic 65.0%
TCTCTACCACGAGGGCCCTG+GGG - chr1_3:56540336-56540355 None:intergenic 65.0%
CTCTCTACCACGAGGGCCCT+GGG - chr1_3:56540337-56540356 None:intergenic 65.0%
ACTCTCTACCACGAGGGCCC+TGG - chr1_3:56540338-56540357 None:intergenic 65.0%
GTGGACCAGTGCGCCAATGG+TGG + chr1_3:56540899-56540918 Msa0127290:CDS 65.0%
TGTGGACCCCAGGGCCCTCG+TGG + chr1_3:56540327-56540346 Msa0127290:CDS 75.0%
TGTGGACCCCAGGGCCCTCG+TGG + chr1_3:56540327-56540346 Msa0127290:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 56540045 56541278 56540045 ID=Msa0127290;Name=Msa0127290
chr1_3 mRNA 56540045 56541278 56540045 ID=Msa0127290-mRNA-1;Parent=Msa0127290;Name=Msa0127290-mRNA-1;_AED=0.39;_eAED=0.39;_QI=63|1|1|1|1|1|2|257|144
chr1_3 exon 56540045 56540377 56540045 ID=Msa0127290-mRNA-1:exon:17868;Parent=Msa0127290-mRNA-1
chr1_3 exon 56540857 56541278 56540857 ID=Msa0127290-mRNA-1:exon:17869;Parent=Msa0127290-mRNA-1
chr1_3 five_prime_UTR 56540045 56540107 56540045 ID=Msa0127290-mRNA-1:five_prime_utr;Parent=Msa0127290-mRNA-1
chr1_3 CDS 56540108 56540377 56540108 ID=Msa0127290-mRNA-1:cds;Parent=Msa0127290-mRNA-1
chr1_3 CDS 56540857 56541021 56540857 ID=Msa0127290-mRNA-1:cds;Parent=Msa0127290-mRNA-1
chr1_3 three_prime_UTR 56541022 56541278 56541022 ID=Msa0127290-mRNA-1:three_prime_utr;Parent=Msa0127290-mRNA-1
Gene Sequence

>Msa0127290

ATGGAGAGCACACAGAGAACTTTGAGCTTGTTGGTTTTGTGTTTCCTTGTTGGAACGATGTTAGTGAATGGTCAGAGTGCAAACGGTGTAAGGTCAACGTACCACCTTTACAACCCTCAGAATATTAATTGGGATTACAACAGAGCCAGTGTATACTGTGCTACCTGGGATGCAAACCAGCCCTTGGAATGGCGTAAAAAGTATGGTTGGACTGCCTTTTGTGGACCCCAGGGCCCTCGTGGTAGAGAGTCTTGTGGCAAGTGCTTGAGGGTGACAAATACTGCAACTGGAGCTCAAGTAACAGTGAGAATAGTGGACCAGTGCGCCAATGGTGGATTAGACTTGGATGTGAATGTCTTCAATCAAATTGATACCAATGGACAGGGTTACCAGAAGGGTCACCTTATAGTTAACTATGTCTTTGTTAATTGTTAA
Protein sequence

>Msa0127290

MESTQRTLSLLVLCFLVGTMLVNGQSANGVRSTYHLYNPQNINWDYNRASVYCATWDANQPLEWRKKYGWTAFCGPQGPRGRESCGKCLRVTNTATGAQVTVRIVDQCANGGLDLDVNVFNQIDTNGQGYQKGHLIVNYVFVNC*