AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0128940


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128940 MtrunA17_Chr1g0195611 92.647 68 5 0 1 68 1 68 2.81e-40 137
Msa0128940 MtrunA17_Chr5g0428831 92.647 68 5 0 1 68 1 68 1.02e-39 135
Msa0128940 MtrunA17_Chr1g0195641 91.176 68 6 0 1 68 1 68 1.97e-31 112
Msa0128940 MtrunA17_Chr4g0006971 76.190 42 10 0 27 68 27 68 8.79e-18 75.1
Msa0128940 MtrunA17_Chr7g0242681 78.049 41 9 0 28 68 39 79 9.91e-18 75.1
Msa0128940 MtrunA17_Chr7g0242691 67.308 52 15 1 19 68 21 72 1.05e-17 75.1
Msa0128940 MtrunA17_Chr3g0088441 60.870 46 18 0 21 66 32 77 1.24e-15 69.3
Msa0128940 MtrunA17_Chr3g0090111 71.795 39 11 0 28 66 20 58 1.59e-15 68.9
Msa0128940 MtrunA17_Chr4g0010501 53.333 60 22 1 7 66 10 63 2.13e-15 68.6
Msa0128940 MtrunA17_Chr8g0352881 82.857 35 6 0 34 68 5 39 2.75e-15 65.5
Msa0128940 MtrunA17_Chr4g0033311 58.696 46 19 0 21 66 33 78 4.12e-15 67.8
Msa0128940 MtrunA17_Chr3g0090131 64.286 42 15 0 25 66 19 60 1.45e-14 66.2
Msa0128940 MtrunA17_Chr4g0033271 64.103 39 14 0 28 66 36 74 7.69e-14 63.9
Msa0128940 MtrunA17_Chr4g0033321 66.667 39 13 0 28 66 34 72 7.85e-14 63.9
Msa0128940 MtrunA17_Chr4g0033341 60.000 45 18 0 22 66 64 108 8.58e-14 63.9
Msa0128940 MtrunA17_Chr3g0087191 55.000 60 24 1 11 67 3 62 9.06e-14 62.0
Msa0128940 MtrunA17_Chr3g0087271 66.667 39 13 0 26 64 37 75 2.76e-13 62.4
Msa0128940 MtrunA17_Chr6g0482951 47.297 74 28 2 2 64 7 80 2.82e-13 62.4
Msa0128940 MtrunA17_Chr6g0482941 47.297 74 28 2 2 64 7 80 3.08e-13 62.4
Msa0128940 MtrunA17_Chr4g0043091 45.000 60 33 0 7 66 8 67 3.11e-13 62.4
Msa0128940 MtrunA17_Chr4g0033331 57.778 45 19 0 22 66 34 78 6.04e-13 61.6
Msa0128940 MtrunA17_Chr3g0087261 66.667 39 13 0 26 64 36 74 9.84e-13 60.8
Msa0128940 MtrunA17_Chr6g0482131 59.524 42 17 0 25 66 34 75 1.29e-12 60.5
Msa0128940 MtrunA17_Chr2g0329191 52.174 46 22 0 21 66 28 73 1.70e-12 60.1
Msa0128940 MtrunA17_Chr4g0033401 64.103 39 14 0 28 66 35 73 1.89e-12 60.1
Msa0128940 MtrunA17_Chr2g0317841 44.444 63 35 0 4 66 9 71 2.15e-12 60.1
Msa0128940 MtrunA17_Chr2g0317821 44.444 63 35 0 4 66 9 71 2.15e-12 60.1
Msa0128940 MtrunA17_Chr4g0033191 60.976 41 16 0 26 66 42 82 2.62e-12 59.7
Msa0128940 MtrunA17_Chr2g0329321 52.174 46 22 0 21 66 32 77 3.33e-12 59.3
Msa0128940 MtrunA17_Chr6g0482081 57.143 42 18 0 25 66 34 75 4.06e-12 59.3
Msa0128940 MtrunA17_Chr4g0033201 44.118 68 27 1 10 66 8 75 6.94e-12 58.5
Msa0128940 MtrunA17_Chr2g0329201 42.623 61 32 1 9 66 10 70 1.43e-11 57.8
Msa0128940 MtrunA17_Chr5g0430451 50.000 46 23 0 21 66 33 78 1.49e-11 57.8
Msa0128940 MtrunA17_Chr6g0482071 45.714 70 23 2 12 66 7 76 1.96e-11 57.4
Msa0128940 MtrunA17_Chr4g0042171 44.828 58 29 1 8 65 8 62 2.00e-11 57.4
Msa0128940 MtrunA17_Chr4g0074551 39.344 61 36 1 7 66 29 89 4.60e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0128940 AT1G02850.3 80.851 47 9 0 22 68 21 67 1.46e-20 83.2
Msa0128940 AT1G02850.5 80.851 47 9 0 22 68 21 67 1.81e-20 83.2
Msa0128940 AT1G02850.4 80.851 47 9 0 22 68 21 67 1.81e-20 83.2
Msa0128940 AT1G02850.2 80.851 47 9 0 22 68 21 67 1.98e-20 83.2
Msa0128940 AT1G02850.1 80.851 47 9 0 22 68 21 67 2.11e-20 83.2
Msa0128940 AT3G62750.3 64.407 59 18 1 11 66 30 88 1.25e-18 77.8
Msa0128940 AT1G45191.1 58.730 63 25 1 5 66 6 68 1.58e-18 77.8
Msa0128940 AT1G45191.6 58.730 63 25 1 5 66 6 68 1.61e-18 77.8
Msa0128940 AT1G45191.2 58.730 63 25 1 5 66 6 68 1.72e-18 77.8
Msa0128940 AT3G62750.2 64.407 59 18 1 11 66 30 88 1.74e-18 77.8
Msa0128940 AT1G45191.4 58.730 63 25 1 5 66 6 68 1.98e-18 77.4
Msa0128940 AT3G62750.1 64.407 59 18 1 11 66 4 62 2.01e-18 77.4
Msa0128940 AT3G62750.4 64.407 59 18 1 11 66 30 88 2.16e-18 77.4
Msa0128940 AT1G45191.5 57.812 64 26 1 5 67 6 69 3.91e-18 76.6
Msa0128940 AT1G45191.3 57.812 64 26 1 5 67 6 69 4.20e-18 76.3
Msa0128940 AT3G62750.7 64.407 59 18 1 11 66 4 62 4.78e-18 76.3
Msa0128940 AT3G62750.8 64.407 59 18 1 11 66 4 62 4.78e-18 76.3
Msa0128940 AT5G42260.1 57.143 63 25 1 5 65 7 69 7.74e-17 72.8
Msa0128940 AT5G44640.1 72.093 43 12 0 23 65 27 69 1.10e-16 72.4
Msa0128940 AT4G27830.1 48.485 66 30 1 1 66 1 62 1.11e-16 72.4
Msa0128940 AT2G44450.1 72.093 43 12 0 23 65 27 69 2.07e-16 71.6
Msa0128940 AT1G60090.1 55.172 58 26 0 10 67 7 64 2.95e-16 71.2
Msa0128940 AT1G60090.2 55.172 58 26 0 10 67 7 64 3.31e-16 71.2
Msa0128940 AT2G25630.1 69.767 43 13 0 23 65 26 68 6.67e-16 70.5
Msa0128940 AT3G60130.1 78.378 37 8 0 29 65 32 68 1.96e-15 68.9
Msa0128940 AT1G60270.1 52.239 67 29 1 1 67 1 64 1.51e-14 66.2
Msa0128940 AT4G27820.1 61.364 44 17 0 24 67 20 63 2.55e-14 65.9
Msa0128940 AT3G62740.1 50.000 56 28 0 11 66 7 62 4.36e-14 65.1
Msa0128940 AT3G62740.2 50.000 56 28 0 11 66 7 62 4.63e-14 65.1
Msa0128940 AT4G22100.1 50.000 66 28 1 1 66 1 61 6.45e-14 64.7
Msa0128940 AT3G18080.1 62.791 43 16 0 22 64 34 76 2.02e-13 63.2
Msa0128940 AT2G44460.1 42.647 68 37 1 1 66 1 68 6.76e-13 61.6
Msa0128940 AT2G44480.4 67.442 43 13 1 22 64 62 103 7.72e-13 61.6
Msa0128940 AT2G44480.3 67.442 43 13 1 22 64 62 103 1.42e-12 60.8
Msa0128940 AT3G18070.1 58.140 43 18 0 22 64 25 67 2.11e-12 60.5
Msa0128940 AT3G18070.3 58.140 43 18 0 22 64 25 67 2.23e-12 60.5
Msa0128940 AT3G18070.4 58.140 43 18 0 22 64 25 67 2.37e-12 60.1
Msa0128940 AT3G18070.2 58.140 43 18 0 22 64 25 67 2.63e-12 60.1
Msa0128940 AT3G18070.5 58.140 43 18 0 22 64 25 67 3.08e-12 59.7
Msa0128940 AT5G24550.1 56.522 46 20 0 22 67 27 72 3.37e-12 59.7
Msa0128940 AT2G44480.1 70.270 37 11 0 28 64 36 72 5.33e-12 59.3
Msa0128940 AT3G60140.1 37.500 56 35 0 10 65 9 64 1.07e-11 58.5
Msa0128940 AT3G60140.2 37.500 56 35 0 10 65 9 64 1.51e-11 57.8
Msa0128940 AT5G36890.1 52.830 53 21 1 14 66 7 55 1.56e-11 57.8
Msa0128940 AT5G36890.2 52.830 53 21 1 14 66 7 55 1.74e-11 57.8
Msa0128940 AT1G52400.3 57.500 40 17 0 25 64 37 76 1.92e-11 57.8
Msa0128940 AT1G52400.1 57.500 40 17 0 25 64 37 76 1.92e-11 57.8
Msa0128940 AT1G52400.2 57.500 40 17 0 25 64 37 76 2.28e-11 57.4
Msa0128940 AT2G44470.3 57.895 38 16 0 29 66 31 68 2.30e-11 57.4
Msa0128940 AT2G44470.1 57.895 38 16 0 29 66 31 68 2.85e-11 57.0
Msa0128940 AT2G44470.2 57.895 38 16 0 29 66 31 68 3.08e-11 57.0
Msa0128940 AT5G24540.2 54.348 46 21 0 22 67 27 72 7.35e-11 55.8
Msa0128940 AT5G24540.1 54.348 46 21 0 22 67 27 72 7.41e-11 55.8

Find 18 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTCTAAACTTGGCTGTT+GGG 0.299049 1_3:+58785142 Msa0128940:CDS
ATCAGGCACCTCTGCATATC+AGG 0.333312 1_3:+58785221 Msa0128940:CDS
ATGGAAGAACTCCTAGCATT+TGG 0.343887 1_3:+58785348 Msa0128940:CDS
TTGCTTCTAAACTTGGCTGT+TGG 0.364143 1_3:+58785141 Msa0128940:CDS
ATAAGTCAGAACCACCAATT+TGG 0.366824 1_3:+58784991 Msa0128940:five_prime_UTR
AGTAAAAGACAACACCAAAT+TGG 0.399116 1_3:-58785005 None:intergenic
TGGAAGAACTCCTAGCATTT+GGG 0.419701 1_3:+58785349 Msa0128940:CDS
TGGGATACCTTTGCACATGC+TGG 0.477610 1_3:+58785368 Msa0128940:CDS
GCTTCTAAACTTGGCTGTTG+GGG 0.486481 1_3:+58785143 Msa0128940:CDS
AAAACATAAAACATAGAAGA+TGG 0.505032 1_3:+58785086 Msa0128940:exon
GAAGGAGCTGCTAATGAAGA+TGG 0.514463 1_3:+58785329 Msa0128940:CDS
ATGGAACACAGAAATGTTTG+AGG 0.535838 1_3:+58785044 Msa0128940:five_prime_UTR
ACAATAGTCAAACATGTATG+TGG 0.562691 1_3:-58785111 None:intergenic
AAAAGACAACACCAAATTGG+TGG 0.601196 1_3:-58785002 None:intergenic
GCAAAGGTATCCCAAATGCT+AGG 0.604783 1_3:-58785359 None:intergenic
TGACTAACCAGCATGTGCAA+AGG 0.633306 1_3:-58785375 None:intergenic
AAAGAGAACCTGATATGCAG+AGG 0.645981 1_3:-58785229 None:intergenic
GATTATGATGAATAGGTGGA+AGG 0.677975 1_3:+58785311 Msa0128940:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACATAAAACATAGAAGA+TGG + chr1_3:58785086-58785105 Msa0128940:exon 20.0%
!! TAAAATACAATGAAATTGCA+TGG - chr1_3:58785278-58785297 None:intergenic 20.0%
!! TTTCTTTTTGCTTCTAAACT+TGG + chr1_3:58785134-58785153 Msa0128940:CDS 25.0%
! TACTTGTGATTATGATGAAT+AGG + chr1_3:58785304-58785323 Msa0128940:intron 25.0%
AGTAAAAGACAACACCAAAT+TGG - chr1_3:58785008-58785027 None:intergenic 30.0%
ACTCACACTCTTCTTAAAAA+TGG + chr1_3:58785025-58785044 Msa0128940:five_prime_UTR 30.0%
ACAATAGTCAAACATGTATG+TGG - chr1_3:58785114-58785133 None:intergenic 30.0%
! TTGCATGGTTACTTCTTTTT+GGG - chr1_3:58785263-58785282 None:intergenic 30.0%
! ATTGCATGGTTACTTCTTTT+TGG - chr1_3:58785264-58785283 None:intergenic 30.0%
TTGTGATTATGATGAATAGG+TGG + chr1_3:58785307-58785326 Msa0128940:intron 30.0%
ATAAGTCAGAACCACCAATT+TGG + chr1_3:58784991-58785010 Msa0128940:five_prime_UTR 35.0%
AAAAGACAACACCAAATTGG+TGG - chr1_3:58785005-58785024 None:intergenic 35.0%
ATGGAACACAGAAATGTTTG+AGG + chr1_3:58785044-58785063 Msa0128940:five_prime_UTR 35.0%
ATCCAAAAACAAAGTCAGCT+GGG - chr1_3:58785203-58785222 None:intergenic 35.0%
! TGCATGGTTACTTCTTTTTG+GGG - chr1_3:58785262-58785281 None:intergenic 35.0%
GATTATGATGAATAGGTGGA+AGG + chr1_3:58785311-58785330 Msa0128940:intron 35.0%
TTGCTTCTAAACTTGGCTGT+TGG + chr1_3:58785141-58785160 Msa0128940:CDS 40.0%
TGCTTCTAAACTTGGCTGTT+GGG + chr1_3:58785142-58785161 Msa0128940:CDS 40.0%
!!! TTCCCAGCTGACTTTGTTTT+TGG + chr1_3:58785198-58785217 Msa0128940:CDS 40.0%
GATCCAAAAACAAAGTCAGC+TGG - chr1_3:58785204-58785223 None:intergenic 40.0%
!!! GCTGACTTTGTTTTTGGATC+AGG + chr1_3:58785204-58785223 Msa0128940:CDS 40.0%
AAAGAGAACCTGATATGCAG+AGG - chr1_3:58785232-58785251 None:intergenic 40.0%
ATGGAAGAACTCCTAGCATT+TGG + chr1_3:58785348-58785367 Msa0128940:CDS 40.0%
TGGAAGAACTCCTAGCATTT+GGG + chr1_3:58785349-58785368 Msa0128940:CDS 40.0%
GCTTCTAAACTTGGCTGTTG+GGG + chr1_3:58785143-58785162 Msa0128940:CDS 45.0%
GAAGGAGCTGCTAATGAAGA+TGG + chr1_3:58785329-58785348 Msa0128940:CDS 45.0%
!! GCAAAGGTATCCCAAATGCT+AGG - chr1_3:58785362-58785381 None:intergenic 45.0%
ATCAGGCACCTCTGCATATC+AGG + chr1_3:58785221-58785240 Msa0128940:CDS 50.0%
TGGGATACCTTTGCACATGC+TGG + chr1_3:58785368-58785387 Msa0128940:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 58784944 58785394 58784944 ID=Msa0128940;Name=Msa0128940
chr1_3 mRNA 58784944 58785394 58784944 ID=Msa0128940-mRNA-1;Parent=Msa0128940;Name=Msa0128940-mRNA-1;_AED=0.48;_eAED=0.48;_QI=161|1|0.5|1|1|1|2|0|68
chr1_3 exon 58784944 58785242 58784944 ID=Msa0128940-mRNA-1:exon:19032;Parent=Msa0128940-mRNA-1
chr1_3 exon 58785326 58785394 58785326 ID=Msa0128940-mRNA-1:exon:19033;Parent=Msa0128940-mRNA-1
chr1_3 five_prime_UTR 58784944 58785104 58784944 ID=Msa0128940-mRNA-1:five_prime_utr;Parent=Msa0128940-mRNA-1
chr1_3 CDS 58785105 58785242 58785105 ID=Msa0128940-mRNA-1:cds;Parent=Msa0128940-mRNA-1
chr1_3 CDS 58785326 58785394 58785326 ID=Msa0128940-mRNA-1:cds;Parent=Msa0128940-mRNA-1
Gene Sequence

>Msa0128940

ATGGAACCACATACATGTTTGACTATTGTTTTCTTTTTGCTTCTAAACTTGGCTGTTGGGGTGTTGAGTACTGATAATTATAGCAGACATGATTTCCCAGCTGACTTTGTTTTTGGATCAGGCACCTCTGCATATCAGGTGGAAGGAGCTGCTAATGAAGATGGAAGAACTCCTAGCATTTGGGATACCTTTGCACATGCTGGTTAG

Protein sequence

>Msa0128940

MEPHTCLTIVFFLLLNLAVGVLSTDNYSRHDFPADFVFGSGTSAYQVEGAANEDGRTPSIWDTFAHAG*