AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705970


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705970 MtrunA17_Chr1g0201251 79.070 43 9 0 1 43 86 128 8.18e-19 76.3
Msa0705970 MtrunA17_Chr6g0466891 72.093 43 12 0 1 43 86 128 2.29e-17 72.4
Msa0705970 MtrunA17_Chr3g0090241 74.419 43 11 0 1 43 86 128 6.80e-16 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705970 AT1G07140.1 56.452 62 24 1 1 59 89 150 6.11e-16 68.9
Msa0705970 AT2G30060.1 53.226 62 26 1 1 59 91 152 1.24e-14 65.5
Msa0705970 AT5G58590.1 53.226 62 26 1 1 59 88 149 5.41e-14 63.9

Find 26 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACCGAGCCGTACTAAAT+TGG 0.252478 4_4:-78824076 None:intergenic
AGCGCAGATCTTGAGGGTTT+TGG 0.290367 4_4:-78824202 None:intergenic
TTAACAGAGTTTCAAGGTTC+TGG 0.300728 4_4:+78824849 Msa0705970:CDS
TGTGGGTGCAGCTGCACTTT+TGG 0.304625 4_4:+78824815 Msa0705970:intron
TGGGAGCTTCTATGTTGTTT+TGG 0.304830 4_4:+78824884 Msa0705970:CDS
ATAAGGTTACTTGAAAAGTT+AGG 0.336456 4_4:+78824163 Msa0705970:five_prime_UTR
GGTTCTGGTGTGTTTATGAT+TGG 0.404939 4_4:+78824864 Msa0705970:CDS
CGATGGAGTTCTTAAGCATA+AGG 0.412330 4_4:+78824146 Msa0705970:five_prime_UTR
GAATTGAGAAATCGTGTGTT+TGG 0.437554 4_4:+78824368 Msa0705970:CDS
GAAAAGTTAGGCTTCTTATG+AGG 0.446917 4_4:+78824175 Msa0705970:exon
TAACTTTATGAGTTCTTGAC+TGG 0.460076 4_4:+78824121 Msa0705970:five_prime_UTR
AATCGTGTGTTTGGCATGCT+AGG 0.466273 4_4:+78824377 Msa0705970:CDS
GTTCTGGTGTGTTTATGATT+GGG 0.482308 4_4:+78824865 Msa0705970:CDS
ACGATTAGCGCAGATCTTGA+GGG 0.489998 4_4:-78824208 None:intergenic
TTCTCTTGCATACTCATCAT+AGG 0.492591 4_4:-78824340 None:intergenic
CAGCGGTTAACAGAGTTTCA+AGG 0.502174 4_4:+78824843 Msa0705970:CDS
GACGATTAGCGCAGATCTTG+AGG 0.502484 4_4:-78824209 None:intergenic
CATGCTAGGGATTCTGCTGA+TGG 0.533927 4_4:+78824391 Msa0705970:CDS
ATCGTGTGTTTGGCATGCTA+GGG 0.537370 4_4:+78824378 Msa0705970:CDS
ATGAGTATGCAAGAGAATGT+TGG 0.553283 4_4:+78824346 Msa0705970:CDS
TGAGTTCTTGACTGGTACGA+TGG 0.554894 4_4:+78824129 Msa0705970:five_prime_UTR
AGCAAAGATATTAGCTGCGG+AGG 0.590198 4_4:+78824051 None:intergenic
GCGGAGGATCCAATTTAGTA+CGG 0.590877 4_4:+78824067 Msa0705970:five_prime_UTR
GGATCCAATTTAGTACGGCT+CGG 0.591535 4_4:+78824072 Msa0705970:five_prime_UTR
AGCAGCAAAGATATTAGCTG+CGG 0.638123 4_4:+78824048 None:intergenic
CCAAGAGGTAGGCATCAGCA+CGG 0.700637 4_4:-78824909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTTAGATGATGTTTTATTT+TGG + chr4_4:78824538-78824557 Msa0705970:intron 20.0%
!!! GTTAGATGATGTTTTATTTT+GGG + chr4_4:78824539-78824558 Msa0705970:intron 20.0%
!!! TTAGATGATGTTTTATTTTG+GGG + chr4_4:78824540-78824559 Msa0705970:intron 20.0%
!!! TGTTAGATGATGTTTTATTT+TGG + chr4_4:78824538-78824557 Msa0705970:intron 20.0%
!!! GTTAGATGATGTTTTATTTT+GGG + chr4_4:78824539-78824558 Msa0705970:intron 20.0%
!!! TTAGATGATGTTTTATTTTG+GGG + chr4_4:78824540-78824559 Msa0705970:intron 20.0%
! ATAAGGTTACTTGAAAAGTT+AGG + chr4_4:78824163-78824182 Msa0705970:five_prime_UTR 25.0%
!!! TTTTGTGATTTTGATTGTTG+TGG + chr4_4:78824294-78824313 Msa0705970:intron 25.0%
!!! CAGATTTTTTGTAGTTAGAT+TGG + chr4_4:78824492-78824511 Msa0705970:intron 25.0%
!! ATTGGTTTAGATGAATATTG+TGG + chr4_4:78824510-78824529 Msa0705970:intron 25.0%
! TTGGTTTAGATGAATATTGT+GGG + chr4_4:78824511-78824530 Msa0705970:intron 25.0%
!! TATTTGGTAATCAAAGAGTT+AGG + chr4_4:78824711-78824730 Msa0705970:intron 25.0%
! ATAAGGTTACTTGAAAAGTT+AGG + chr4_4:78824163-78824182 Msa0705970:five_prime_UTR 25.0%
!!! TTTTGTGATTTTGATTGTTG+TGG + chr4_4:78824294-78824313 Msa0705970:intron 25.0%
!!! CAGATTTTTTGTAGTTAGAT+TGG + chr4_4:78824492-78824511 Msa0705970:intron 25.0%
!! ATTGGTTTAGATGAATATTG+TGG + chr4_4:78824510-78824529 Msa0705970:intron 25.0%
! TTGGTTTAGATGAATATTGT+GGG + chr4_4:78824511-78824530 Msa0705970:intron 25.0%
!! TATTTGGTAATCAAAGAGTT+AGG + chr4_4:78824711-78824730 Msa0705970:intron 25.0%
TAACTTTATGAGTTCTTGAC+TGG + chr4_4:78824121-78824140 Msa0705970:five_prime_UTR 30.0%
TGATAACTATCTTGTTAGAG+TGG + chr4_4:78824663-78824682 Msa0705970:intron 30.0%
TAACTTTATGAGTTCTTGAC+TGG + chr4_4:78824121-78824140 Msa0705970:five_prime_UTR 30.0%
TGATAACTATCTTGTTAGAG+TGG + chr4_4:78824663-78824682 Msa0705970:intron 30.0%
GAAAAGTTAGGCTTCTTATG+AGG + chr4_4:78824175-78824194 Msa0705970:exon 35.0%
TTCTCTTGCATACTCATCAT+AGG - chr4_4:78824343-78824362 None:intergenic 35.0%
ATGAGTATGCAAGAGAATGT+TGG + chr4_4:78824346-78824365 Msa0705970:CDS 35.0%
GAATTGAGAAATCGTGTGTT+TGG + chr4_4:78824368-78824387 Msa0705970:CDS 35.0%
! AGGATGCACTTTTCTGTTTT+CGG + chr4_4:78824423-78824442 Msa0705970:intron 35.0%
ACTCAACAACTATTGACATG+TGG + chr4_4:78824608-78824627 Msa0705970:intron 35.0%
! TTGGTAATCAAAGAGTTAGG+TGG + chr4_4:78824714-78824733 Msa0705970:intron 35.0%
!!! TGTTAGAGTTTTTGCTGTTG+TGG + chr4_4:78824797-78824816 Msa0705970:intron 35.0%
!!! GTTAGAGTTTTTGCTGTTGT+GGG + chr4_4:78824798-78824817 Msa0705970:intron 35.0%
TTAACAGAGTTTCAAGGTTC+TGG + chr4_4:78824849-78824868 Msa0705970:CDS 35.0%
GTTCTGGTGTGTTTATGATT+GGG + chr4_4:78824865-78824884 Msa0705970:CDS 35.0%
GAAAAGTTAGGCTTCTTATG+AGG + chr4_4:78824175-78824194 Msa0705970:exon 35.0%
TTCTCTTGCATACTCATCAT+AGG - chr4_4:78824343-78824362 None:intergenic 35.0%
ATGAGTATGCAAGAGAATGT+TGG + chr4_4:78824346-78824365 Msa0705970:CDS 35.0%
GAATTGAGAAATCGTGTGTT+TGG + chr4_4:78824368-78824387 Msa0705970:CDS 35.0%
! AGGATGCACTTTTCTGTTTT+CGG + chr4_4:78824423-78824442 Msa0705970:intron 35.0%
ACTCAACAACTATTGACATG+TGG + chr4_4:78824608-78824627 Msa0705970:intron 35.0%
! TTGGTAATCAAAGAGTTAGG+TGG + chr4_4:78824714-78824733 Msa0705970:intron 35.0%
!!! TGTTAGAGTTTTTGCTGTTG+TGG + chr4_4:78824797-78824816 Msa0705970:intron 35.0%
!!! GTTAGAGTTTTTGCTGTTGT+GGG + chr4_4:78824798-78824817 Msa0705970:intron 35.0%
TTAACAGAGTTTCAAGGTTC+TGG + chr4_4:78824849-78824868 Msa0705970:CDS 35.0%
GTTCTGGTGTGTTTATGATT+GGG + chr4_4:78824865-78824884 Msa0705970:CDS 35.0%
CGATGGAGTTCTTAAGCATA+AGG + chr4_4:78824146-78824165 Msa0705970:five_prime_UTR 40.0%
CTATCTTGTTAGAGTGGTGT+TGG + chr4_4:78824669-78824688 Msa0705970:intron 40.0%
AATCAAAGAGTTAGGTGGTG+CGG + chr4_4:78824719-78824738 Msa0705970:intron 40.0%
! GGTTCTGGTGTGTTTATGAT+TGG + chr4_4:78824864-78824883 Msa0705970:CDS 40.0%
!! TGGGAGCTTCTATGTTGTTT+TGG + chr4_4:78824884-78824903 Msa0705970:CDS 40.0%
CGATGGAGTTCTTAAGCATA+AGG + chr4_4:78824146-78824165 Msa0705970:five_prime_UTR 40.0%
CTATCTTGTTAGAGTGGTGT+TGG + chr4_4:78824669-78824688 Msa0705970:intron 40.0%
AATCAAAGAGTTAGGTGGTG+CGG + chr4_4:78824719-78824738 Msa0705970:intron 40.0%
! GGTTCTGGTGTGTTTATGAT+TGG + chr4_4:78824864-78824883 Msa0705970:CDS 40.0%
!! TGGGAGCTTCTATGTTGTTT+TGG + chr4_4:78824884-78824903 Msa0705970:CDS 40.0%
GCGGAGGATCCAATTTAGTA+CGG + chr4_4:78824067-78824086 Msa0705970:five_prime_UTR 45.0%
GGATCCAATTTAGTACGGCT+CGG + chr4_4:78824072-78824091 Msa0705970:five_prime_UTR 45.0%
ACAACCGAGCCGTACTAAAT+TGG - chr4_4:78824079-78824098 None:intergenic 45.0%
TGAGTTCTTGACTGGTACGA+TGG + chr4_4:78824129-78824148 Msa0705970:five_prime_UTR 45.0%
ACGATTAGCGCAGATCTTGA+GGG - chr4_4:78824211-78824230 None:intergenic 45.0%
AATCGTGTGTTTGGCATGCT+AGG + chr4_4:78824377-78824396 Msa0705970:CDS 45.0%
ATCGTGTGTTTGGCATGCTA+GGG + chr4_4:78824378-78824397 Msa0705970:CDS 45.0%
! TCTGCTGATGGTGAGCTTAA+AGG + chr4_4:78824403-78824422 Msa0705970:intron 45.0%
ATCAAAGAGTTAGGTGGTGC+GGG + chr4_4:78824720-78824739 Msa0705970:intron 45.0%
AGACTAGTGTTGTAGCTGCA+TGG + chr4_4:78824758-78824777 Msa0705970:intron 45.0%
CAGCGGTTAACAGAGTTTCA+AGG + chr4_4:78824843-78824862 Msa0705970:CDS 45.0%
GCGGAGGATCCAATTTAGTA+CGG + chr4_4:78824067-78824086 Msa0705970:five_prime_UTR 45.0%
GGATCCAATTTAGTACGGCT+CGG + chr4_4:78824072-78824091 Msa0705970:five_prime_UTR 45.0%
ACAACCGAGCCGTACTAAAT+TGG - chr4_4:78824079-78824098 None:intergenic 45.0%
TGAGTTCTTGACTGGTACGA+TGG + chr4_4:78824129-78824148 Msa0705970:five_prime_UTR 45.0%
ACGATTAGCGCAGATCTTGA+GGG - chr4_4:78824211-78824230 None:intergenic 45.0%
AATCGTGTGTTTGGCATGCT+AGG + chr4_4:78824377-78824396 Msa0705970:CDS 45.0%
ATCGTGTGTTTGGCATGCTA+GGG + chr4_4:78824378-78824397 Msa0705970:CDS 45.0%
! TCTGCTGATGGTGAGCTTAA+AGG + chr4_4:78824403-78824422 Msa0705970:intron 45.0%
ATCAAAGAGTTAGGTGGTGC+GGG + chr4_4:78824720-78824739 Msa0705970:intron 45.0%
AGACTAGTGTTGTAGCTGCA+TGG + chr4_4:78824758-78824777 Msa0705970:intron 45.0%
CAGCGGTTAACAGAGTTTCA+AGG + chr4_4:78824843-78824862 Msa0705970:CDS 45.0%
! AGCGCAGATCTTGAGGGTTT+TGG - chr4_4:78824205-78824224 None:intergenic 50.0%
GACGATTAGCGCAGATCTTG+AGG - chr4_4:78824212-78824231 None:intergenic 50.0%
CATGCTAGGGATTCTGCTGA+TGG + chr4_4:78824391-78824410 Msa0705970:CDS 50.0%
! TAGAGTGGTGTTGGAATCCG+AGG + chr4_4:78824678-78824697 Msa0705970:intron 50.0%
! AGAGTGGTGTTGGAATCCGA+GGG + chr4_4:78824679-78824698 Msa0705970:intron 50.0%
TGTTGGAATCCGAGGGAGTA+GGG + chr4_4:78824686-78824705 Msa0705970:intron 50.0%
CCAAATACACCCTACTCCCT+CGG - chr4_4:78824698-78824717 None:intergenic 50.0%
!! GTGCAGCTGCACTTTTGGTT+TGG + chr4_4:78824820-78824839 Msa0705970:intron 50.0%
!!! CTGCACTTTTGGTTTGGCAG+CGG + chr4_4:78824826-78824845 Msa0705970:CDS 50.0%
! AGCGCAGATCTTGAGGGTTT+TGG - chr4_4:78824205-78824224 None:intergenic 50.0%
GACGATTAGCGCAGATCTTG+AGG - chr4_4:78824212-78824231 None:intergenic 50.0%
CATGCTAGGGATTCTGCTGA+TGG + chr4_4:78824391-78824410 Msa0705970:CDS 50.0%
! TAGAGTGGTGTTGGAATCCG+AGG + chr4_4:78824678-78824697 Msa0705970:intron 50.0%
! AGAGTGGTGTTGGAATCCGA+GGG + chr4_4:78824679-78824698 Msa0705970:intron 50.0%
TGTTGGAATCCGAGGGAGTA+GGG + chr4_4:78824686-78824705 Msa0705970:intron 50.0%
CCAAATACACCCTACTCCCT+CGG - chr4_4:78824698-78824717 None:intergenic 50.0%
!! GTGCAGCTGCACTTTTGGTT+TGG + chr4_4:78824820-78824839 Msa0705970:intron 50.0%
!!! CTGCACTTTTGGTTTGGCAG+CGG + chr4_4:78824826-78824845 Msa0705970:CDS 50.0%
GTGTTGGAATCCGAGGGAGT+AGG + chr4_4:78824685-78824704 Msa0705970:intron 55.0%
CCGAGGGAGTAGGGTGTATT+TGG + chr4_4:78824695-78824714 Msa0705970:intron 55.0%
AAGAGTTAGGTGGTGCGGGA+AGG + chr4_4:78824724-78824743 Msa0705970:intron 55.0%
AGAGTTAGGTGGTGCGGGAA+GGG + chr4_4:78824725-78824744 Msa0705970:intron 55.0%
! TGTGGGTGCAGCTGCACTTT+TGG + chr4_4:78824815-78824834 Msa0705970:intron 55.0%
GTGTTGGAATCCGAGGGAGT+AGG + chr4_4:78824685-78824704 Msa0705970:intron 55.0%
CCGAGGGAGTAGGGTGTATT+TGG + chr4_4:78824695-78824714 Msa0705970:intron 55.0%
AAGAGTTAGGTGGTGCGGGA+AGG + chr4_4:78824724-78824743 Msa0705970:intron 55.0%
AGAGTTAGGTGGTGCGGGAA+GGG + chr4_4:78824725-78824744 Msa0705970:intron 55.0%
! TGTGGGTGCAGCTGCACTTT+TGG + chr4_4:78824815-78824834 Msa0705970:intron 55.0%
GAGTTAGGTGGTGCGGGAAG+GGG + chr4_4:78824726-78824745 Msa0705970:intron 60.0%
AGTTAGGTGGTGCGGGAAGG+GGG + chr4_4:78824727-78824746 Msa0705970:intron 60.0%
GAGTTAGGTGGTGCGGGAAG+GGG + chr4_4:78824726-78824745 Msa0705970:intron 60.0%
AGTTAGGTGGTGCGGGAAGG+GGG + chr4_4:78824727-78824746 Msa0705970:intron 60.0%
GTTAGGTGGTGCGGGAAGGG+GGG + chr4_4:78824728-78824747 Msa0705970:intron 65.0%
GTTAGGTGGTGCGGGAAGGG+GGG + chr4_4:78824728-78824747 Msa0705970:intron 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 78824057 78824917 78824057 ID=Msa0705970;Name=Msa0705970
chr4_4 mRNA 78824057 78824917 78824057 ID=Msa0705970-mRNA-1;Parent=Msa0705970;Name=Msa0705970-mRNA-1;_AED=0.44;_eAED=0.44;_QI=135|0.5|0.33|0.66|0.5|0.66|3|0|70
chr4_4 exon 78824057 78824234 78824057 ID=Msa0705970-mRNA-1:exon:25244;Parent=Msa0705970-mRNA-1
chr4_4 exon 78824335 78824412 78824335 ID=Msa0705970-mRNA-1:exon:25245;Parent=Msa0705970-mRNA-1
chr4_4 exon 78824826 78824917 78824826 ID=Msa0705970-mRNA-1:exon:25246;Parent=Msa0705970-mRNA-1
chr4_4 five_prime_UTR 78824057 78824191 78824057 ID=Msa0705970-mRNA-1:five_prime_utr;Parent=Msa0705970-mRNA-1
chr4_4 CDS 78824192 78824234 78824192 ID=Msa0705970-mRNA-1:cds;Parent=Msa0705970-mRNA-1
chr4_4 CDS 78824335 78824412 78824335 ID=Msa0705970-mRNA-1:cds;Parent=Msa0705970-mRNA-1
chr4_4 CDS 78824826 78824917 78824826 ID=Msa0705970-mRNA-1:cds;Parent=Msa0705970-mRNA-1
Gene Sequence

>Msa0705970

ATGAGGCAATCCAAAACCCTCAAGATCTGCGCTAATCGTCTTATTATACCTATGATGAGTATGCAAGAGAATGTTGGAATTGAGAAATCGTGTGTTTGGCATGCTAGGGATTCTGCTGATGCTGCACTTTTGGTTTGGCAGCGGTTAACAGAGTTTCAAGGTTCTGGTGTGTTTATGATTGGGAGCTTCTATGTTGTTTTGGTTCCGTGCTGA

Protein sequence

>Msa0705970

MRQSKTLKICANRLIIPMMSMQENVGIEKSCVWHARDSADAALLVWQRLTEFQGSGVFMIGSFYVVLVPC*