AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0174950

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174950 MtrunA17_Chr1g0195811 90.052 191 19 0 2 192 24 214 7.76e-129 360
Msa0174950 MtrunA17_Chr1g0195821 86.702 188 25 0 5 192 27 214 3.77e-121 341
Msa0174950 MtrunA17_Chr3g0109891 44.920 187 102 1 1 186 22 208 5.04e-52 166
Msa0174950 MtrunA17_Chr3g0095781 44.865 185 101 1 6 189 26 210 1.05e-51 165
Msa0174950 MtrunA17_Chr1g0195831 40.217 184 109 1 6 188 27 210 1.53e-49 160
Msa0174950 MtrunA17_Chr5g0442751 43.158 190 104 3 1 187 21 209 4.39e-49 158
Msa0174950 MtrunA17_Chr5g0442741 40.838 191 111 2 1 189 21 211 1.56e-48 157
Msa0174950 MtrunA17_Chr3g0095821 45.122 164 89 1 27 189 1 164 2.45e-47 152
Msa0174950 MtrunA17_Chr1g0157211 40.415 193 109 3 5 191 28 220 1.93e-46 152
Msa0174950 MtrunA17_Chr1g0198101 86.765 68 9 0 125 192 1 68 3.40e-39 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174950 AT1G02920.1 63.388 183 67 0 5 187 27 209 8.20e-78 231
Msa0174950 AT1G02930.2 62.088 182 68 1 6 187 28 208 1.91e-76 228
Msa0174950 AT1G02930.1 62.088 182 68 1 6 187 28 208 1.91e-76 228
Msa0174950 AT4G02520.1 59.459 185 72 1 5 186 27 211 1.16e-74 224
Msa0174950 AT2G47730.2 55.435 184 81 1 5 187 75 258 6.98e-73 221
Msa0174950 AT2G47730.1 55.435 184 81 1 5 187 75 258 6.98e-73 221
Msa0174950 AT2G02930.1 59.140 186 73 1 5 187 27 212 1.64e-72 218
Msa0174950 AT1G02950.5 54.144 181 82 1 4 183 59 239 2.60e-67 206
Msa0174950 AT1G02950.3 54.144 181 82 1 4 183 47 227 2.66e-67 206
Msa0174950 AT1G02950.1 54.144 181 82 1 4 183 47 227 2.66e-67 206
Msa0174950 AT1G02950.2 54.144 181 82 1 4 183 49 229 3.05e-67 206
Msa0174950 AT3G62760.1 50.000 182 90 1 6 186 27 208 2.03e-57 180
Msa0174950 AT1G49860.1 48.148 189 94 2 5 189 28 216 8.29e-57 180
Msa0174950 AT1G02940.4 45.652 184 99 1 5 187 62 245 1.50e-56 179
Msa0174950 AT1G02940.1 45.652 184 99 1 5 187 62 245 1.50e-56 179
Msa0174950 AT1G02940.3 45.652 184 99 1 5 187 75 258 1.87e-56 179
Msa0174950 AT1G02940.2 45.652 184 99 1 5 187 87 270 1.97e-56 179
Msa0174950 AT3G03190.1 45.699 186 100 1 5 189 26 211 7.49e-52 166
Msa0174950 AT5G17220.1 39.474 190 114 1 1 189 22 211 9.39e-47 153
Msa0174950 AT2G30860.1 40.984 183 107 1 7 188 27 209 3.62e-45 149
Msa0174950 AT1G02950.4 50.704 142 69 1 43 183 60 201 2.29e-44 147
Msa0174950 AT2G30870.1 39.344 183 110 1 7 188 27 209 5.24e-42 140
Msa0174950 AT2G30860.2 37.795 127 79 0 7 133 27 153 2.91e-26 99.0
Msa0174950 AT5G41240.1 30.387 181 114 5 3 174 24 201 5.56e-11 61.2

Find 64 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTCACTTCGATTATTTA+TGG 0.098554 1_4:+60722419 None:intergenic
CCCCATTATTGCTATCTTCT+TGG 0.169803 1_4:+60722871 None:intergenic
TTGGGGATAAAGAGTTTATT+TGG 0.186179 1_4:-60722788 Msa0174950:CDS
TTTCAAGTGTACTGTAAAAT+AGG 0.210728 1_4:-60722212 Msa0174950:three_prime_UTR
ATCAAGAATGCTATCCAATT+TGG 0.237155 1_4:+60722727 None:intergenic
AGAGTCACCTCCCAAGTATT+TGG 0.266829 1_4:+60722679 None:intergenic
CTCCAAGAAGATAGCAATAA+TGG 0.269830 1_4:-60722873 Msa0174950:CDS
TCAAAACTTGTTCGGGAATT+GGG 0.278093 1_4:-60722806 Msa0174950:CDS
ATCAAAACTTGTTCGGGAAT+TGG 0.285433 1_4:-60722807 Msa0174950:CDS
TGAAGAGAATGAAGCCAAAT+TGG 0.299438 1_4:-60722741 Msa0174950:CDS
TCGTGATTCAAACAATTTCT+TGG 0.300130 1_4:+60722598 None:intergenic
CGAATCAAAGACATTGGTTA+AGG 0.340841 1_4:-60722337 Msa0174950:three_prime_UTR
GAAGAATGGAGAACACAAAA+AGG 0.349354 1_4:-60723474 Msa0174950:CDS
GGAGTCATTGCTTGTTAACT+TGG 0.352335 1_4:+60722892 None:intergenic
CGCATGGTGGCCGTTGAGTA+GGG 0.361457 1_4:+60723555 None:intergenic
AACAATGAGTATGCTGATAA+GGG 0.368137 1_4:-60722917 Msa0174950:CDS
TTCTTTATGTTGCAGCCATT+TGG 0.379956 1_4:-60723254 Msa0174950:intron
AATGTAATATATTGATTTGT+TGG 0.385404 1_4:-60722290 Msa0174950:three_prime_UTR
TAACAATGAGTATGCTGATA+AGG 0.387390 1_4:-60722918 Msa0174950:CDS
AGTAGCATCAAAACTTGTTC+GGG 0.393847 1_4:-60722813 Msa0174950:CDS
AAGTAGCATCAAAACTTGTT+CGG 0.398448 1_4:-60722814 Msa0174950:CDS
GAGTTTATTTGGAATTCCTT+TGG 0.399412 1_4:-60722777 Msa0174950:CDS
CCGCATGGTGGCCGTTGAGT+AGG 0.403862 1_4:+60723554 None:intergenic
TCCAAGAAGATAGCAATAAT+GGG 0.404293 1_4:-60722872 Msa0174950:CDS
GTTAACATTAACTTGAAGAA+TGG 0.418899 1_4:-60723488 Msa0174950:CDS
AGGTGACTCTTTCACCTTGG+TGG 0.445525 1_4:-60722666 Msa0174950:CDS
AAATCTCCATCTTCAAAAGC+TGG 0.446504 1_4:+60723224 None:intergenic
ACTGTCTTAATTAGGTATCA+TGG 0.449391 1_4:+60722514 None:intergenic
GAATCAAAGACATTGGTTAA+GGG 0.449959 1_4:-60722336 Msa0174950:three_prime_UTR
GCTTACATTGAGTGATATGA+AGG 0.454820 1_4:+60723448 None:intergenic
CACGATCTCATGTTAGTGCT+TGG 0.456189 1_4:-60722580 Msa0174950:CDS
AAAATTGAAATGCAGAATCA+AGG 0.457301 1_4:-60722958 Msa0174950:intron
ATTGTCTGAGTCCAAATACT+TGG 0.458455 1_4:-60722690 Msa0174950:CDS
ATGTAATATATTGATTTGTT+GGG 0.460128 1_4:-60722289 Msa0174950:three_prime_UTR
GATGGAGATTTAAAGCTCTT+CGG 0.472467 1_4:-60723212 Msa0174950:intron
AAAGCTGGAACTTGACCAAA+TGG 0.480424 1_4:+60723239 None:intergenic
ACGATCTCATGTTAGTGCTT+GGG 0.482947 1_4:-60722579 Msa0174950:CDS
TTGGTTAAGGGATTTGAGTC+CGG 0.493988 1_4:-60722324 Msa0174950:three_prime_UTR
GTGATTGATCTGCTAAATCA+TGG 0.497615 1_4:-60723601 Msa0174950:five_prime_UTR
ATGCTACTTGATCGAAGTGT+TGG 0.510802 1_4:+60722828 None:intergenic
GGGAGGTGACTCTTTCACCT+TGG 0.520363 1_4:-60722669 Msa0174950:CDS
TTCATCAAGTAATGAAAAGT+AGG 0.527654 1_4:+60722629 None:intergenic
CTACTCAACGGCCACCATGC+GGG 0.529590 1_4:-60723553 Msa0174950:five_prime_UTR
CAAAACTTGTTCGGGAATTG+GGG 0.535983 1_4:-60722805 Msa0174950:CDS
GAATGAAGATTCTTCACTGA+AGG 0.551224 1_4:+60722390 None:intergenic
CTTAATTAGGTATCATGGCA+AGG 0.566951 1_4:+60722519 None:intergenic
AAATTGAAATGCAGAATCAA+GGG 0.575285 1_4:-60722957 Msa0174950:intron
GCAAGGACTTTAGACCAAGC+TGG 0.580505 1_4:+60722536 None:intergenic
GCATGGTGGCCGTTGAGTAG+GGG 0.589594 1_4:+60723556 None:intergenic
ATAACTCGAATCAAAGACAT+TGG 0.594893 1_4:-60722343 Msa0174950:three_prime_UTR
ATATCACTGCAAGACCAGCT+TGG 0.601324 1_4:-60722550 Msa0174950:CDS
TTGTCTGAGTCCAAATACTT+GGG 0.601763 1_4:-60722689 Msa0174950:CDS
ATCTCATGTTAGTGCTTGGG+TGG 0.606175 1_4:-60722576 Msa0174950:CDS
CCAAGAAGATAGCAATAATG+GGG 0.612634 1_4:-60722871 Msa0174950:CDS
CTTACATTGAGTGATATGAA+GGG 0.613061 1_4:+60723449 None:intergenic
GCACGGAAGCCCCTACTCAA+CGG 0.627204 1_4:-60723565 Msa0174950:five_prime_UTR
ACAATGAGTATGCTGATAAG+GGG 0.629847 1_4:-60722916 Msa0174950:CDS
ATGGCATCAATAAAAGTGCA+CGG 0.642318 1_4:-60723582 Msa0174950:five_prime_UTR
CAGAGTTGCAGTAACCCGCA+TGG 0.653094 1_4:+60723539 None:intergenic
CCTACTCAACGGCCACCATG+CGG 0.660998 1_4:-60723554 Msa0174950:five_prime_UTR
TCTGAGTCCAAATACTTGGG+AGG 0.681272 1_4:-60722686 Msa0174950:CDS
CAAGAAGATAGCAATAATGG+GGG 0.701769 1_4:-60722870 Msa0174950:CDS
TAAATGATGCAAATCCACCA+AGG 0.702241 1_4:+60722652 None:intergenic
AGTTGCAGTAACCCGCATGG+TGG 0.707787 1_4:+60723542 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATACATTGATAATTTAACAT+TGG + chr1_4:60722728-60722747 None:intergenic 15.0%
!!! CAATATATTACATTTTTGAA+AGG + chr1_4:60723495-60723514 None:intergenic 15.0%
!!! AATGTAATATATTGATTTGT+TGG - chr1_4:60723501-60723520 Msa0174950:CDS 15.0%
!!! ATGTAATATATTGATTTGTT+GGG - chr1_4:60723502-60723521 Msa0174950:CDS 15.0%
!!! ATACATTGATAATTTAACAT+TGG + chr1_4:60722728-60722747 None:intergenic 15.0%
!!! CAATATATTACATTTTTGAA+AGG + chr1_4:60723495-60723514 None:intergenic 15.0%
!!! AATGTAATATATTGATTTGT+TGG - chr1_4:60723501-60723520 Msa0174950:CDS 15.0%
!!! ATGTAATATATTGATTTGTT+GGG - chr1_4:60723502-60723521 Msa0174950:CDS 15.0%
!!! TTGTTTTTCAATACTGTTTT+TGG - chr1_4:60723326-60723345 Msa0174950:intron 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG + chr1_4:60723443-60723462 None:intergenic 20.0%
!!! TTGTTTTTCAATACTGTTTT+TGG - chr1_4:60723326-60723345 Msa0174950:intron 20.0%
!!! TTTGATTCGAGTTATTTTTA+TGG + chr1_4:60723443-60723462 None:intergenic 20.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1_4:60722303-60722322 Msa0174950:three_prime_UTR 25.0%
!! ATCAATTAGTTTGATCAATG+TGG + chr1_4:60722514-60722533 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1_4:60722833-60722852 Msa0174950:CDS 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1_4:60722834-60722853 Msa0174950:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1_4:60723042-60723061 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1_4:60723165-60723184 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG - chr1_4:60723283-60723302 Msa0174950:intron 25.0%
! ATCCAAAAACTGTCTTAATT+AGG + chr1_4:60723288-60723307 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG + chr1_4:60723489-60723508 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG + chr1_4:60723570-60723589 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG - chr1_4:60723579-60723598 Msa0174950:five_prime_UTR 25.0%
!! GTTAACATTAACTTGAAGAA+TGG - chr1_4:60722303-60722322 Msa0174950:three_prime_UTR 25.0%
!! ATCAATTAGTTTGATCAATG+TGG + chr1_4:60722514-60722533 None:intergenic 25.0%
! AAAATTGAAATGCAGAATCA+AGG - chr1_4:60722833-60722852 Msa0174950:CDS 25.0%
! AAATTGAAATGCAGAATCAA+GGG - chr1_4:60722834-60722853 Msa0174950:CDS 25.0%
!! TCATTCTCTTCAACAATTTT+TGG + chr1_4:60723042-60723061 None:intergenic 25.0%
! TTCATCAAGTAATGAAAAGT+AGG + chr1_4:60723165-60723184 None:intergenic 25.0%
!! TACCTAATTAAGACAGTTTT+TGG - chr1_4:60723283-60723302 Msa0174950:intron 25.0%
! ATCCAAAAACTGTCTTAATT+AGG + chr1_4:60723288-60723307 None:intergenic 25.0%
!!! ATTACATTTTTGAAAGGAAC+CGG + chr1_4:60723489-60723508 None:intergenic 25.0%
! TACACTTGAAAAATGACAAT+AGG + chr1_4:60723570-60723589 None:intergenic 25.0%
!! TTTCAAGTGTACTGTAAAAT+AGG - chr1_4:60723579-60723598 Msa0174950:five_prime_UTR 25.0%
CTTACATTGAGTGATATGAA+GGG + chr1_4:60722345-60722364 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG + chr1_4:60722510-60722529 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG + chr1_4:60722685-60722704 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1_4:60722873-60722892 Msa0174950:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG - chr1_4:60722874-60722893 Msa0174950:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1_4:60722919-60722938 Msa0174950:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1_4:60722977-60722996 Msa0174950:intron 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1_4:60723003-60723022 Msa0174950:intron 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1_4:60723014-60723033 Msa0174950:intron 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1_4:60723033-60723052 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG + chr1_4:60723067-60723086 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1_4:60723196-60723215 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG + chr1_4:60723280-60723299 None:intergenic 30.0%
ACTCTCACTTCGATTATTTA+TGG + chr1_4:60723375-60723394 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG + chr1_4:60723429-60723448 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG - chr1_4:60723448-60723467 Msa0174950:intron 30.0%
! GAATCAAAGACATTGGTTAA+GGG - chr1_4:60723455-60723474 Msa0174950:CDS 30.0%
CTTACATTGAGTGATATGAA+GGG + chr1_4:60722345-60722364 None:intergenic 30.0%
! ATTAGTTTGATCAATGTGGT+TGG + chr1_4:60722510-60722529 None:intergenic 30.0%
!!! GTTTCTTTTCATTTTTTGCG+AGG + chr1_4:60722685-60722704 None:intergenic 30.0%
TAACAATGAGTATGCTGATA+AGG - chr1_4:60722873-60722892 Msa0174950:CDS 30.0%
AACAATGAGTATGCTGATAA+GGG - chr1_4:60722874-60722893 Msa0174950:CDS 30.0%
TCCAAGAAGATAGCAATAAT+GGG - chr1_4:60722919-60722938 Msa0174950:CDS 30.0%
! AAGTAGCATCAAAACTTGTT+CGG - chr1_4:60722977-60722996 Msa0174950:intron 30.0%
TTGGGGATAAAGAGTTTATT+TGG - chr1_4:60723003-60723022 Msa0174950:intron 30.0%
! GAGTTTATTTGGAATTCCTT+TGG - chr1_4:60723014-60723033 Msa0174950:intron 30.0%
!! TCAACAATTTTTGGATCCAA+AGG + chr1_4:60723033-60723052 None:intergenic 30.0%
ATCAAGAATGCTATCCAATT+TGG + chr1_4:60723067-60723086 None:intergenic 30.0%
TCGTGATTCAAACAATTTCT+TGG + chr1_4:60723196-60723215 None:intergenic 30.0%
ACTGTCTTAATTAGGTATCA+TGG + chr1_4:60723280-60723299 None:intergenic 30.0%
ACTCTCACTTCGATTATTTA+TGG + chr1_4:60723375-60723394 None:intergenic 30.0%
! TTTTTATGGAAATGTGTGCT+TGG + chr1_4:60723429-60723448 None:intergenic 30.0%
ATAACTCGAATCAAAGACAT+TGG - chr1_4:60723448-60723467 Msa0174950:intron 30.0%
! GAATCAAAGACATTGGTTAA+GGG - chr1_4:60723455-60723474 Msa0174950:CDS 30.0%
GTGATTGATCTGCTAAATCA+TGG - chr1_4:60722190-60722209 Msa0174950:three_prime_UTR 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1_4:60722209-60722228 Msa0174950:three_prime_UTR 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1_4:60722317-60722336 Msa0174950:three_prime_UTR 35.0%
GCTTACATTGAGTGATATGA+AGG + chr1_4:60722346-60722365 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1_4:60722537-60722556 Msa0174950:CDS 35.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1_4:60722570-60722589 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+CGG - chr1_4:60722579-60722598 Msa0174950:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG + chr1_4:60722673-60722692 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1_4:60722875-60722894 Msa0174950:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1_4:60722918-60722937 Msa0174950:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1_4:60722920-60722939 Msa0174950:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1_4:60722921-60722940 Msa0174950:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1_4:60722978-60722997 Msa0174950:intron 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1_4:60722984-60723003 Msa0174950:intron 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1_4:60722985-60723004 Msa0174950:intron 35.0%
TGAAGAGAATGAAGCCAAAT+TGG - chr1_4:60723050-60723069 Msa0174950:intron 35.0%
ATTGTCTGAGTCCAAATACT+TGG - chr1_4:60723101-60723120 Msa0174950:intron 35.0%
TTGTCTGAGTCCAAATACTT+GGG - chr1_4:60723102-60723121 Msa0174950:intron 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1_4:60723142-60723161 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG + chr1_4:60723275-60723294 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG + chr1_4:60723404-60723423 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG - chr1_4:60723454-60723473 Msa0174950:CDS 35.0%
GTGATTGATCTGCTAAATCA+TGG - chr1_4:60722190-60722209 Msa0174950:three_prime_UTR 35.0%
! ATGGCATCAATAAAAGTGCA+CGG - chr1_4:60722209-60722228 Msa0174950:three_prime_UTR 35.0%
GAAGAATGGAGAACACAAAA+AGG - chr1_4:60722317-60722336 Msa0174950:three_prime_UTR 35.0%
GCTTACATTGAGTGATATGA+AGG + chr1_4:60722346-60722365 None:intergenic 35.0%
TTCTTTATGTTGCAGCCATT+TGG - chr1_4:60722537-60722556 Msa0174950:CDS 35.0%
AAATCTCCATCTTCAAAAGC+TGG + chr1_4:60722570-60722589 None:intergenic 35.0%
! GATGGAGATTTAAAGCTCTT+CGG - chr1_4:60722579-60722598 Msa0174950:CDS 35.0%
! TTTTTGCGAGGAGTGAAATT+AGG + chr1_4:60722673-60722692 None:intergenic 35.0%
ACAATGAGTATGCTGATAAG+GGG - chr1_4:60722875-60722894 Msa0174950:CDS 35.0%
CTCCAAGAAGATAGCAATAA+TGG - chr1_4:60722918-60722937 Msa0174950:CDS 35.0%
CCAAGAAGATAGCAATAATG+GGG - chr1_4:60722920-60722939 Msa0174950:CDS 35.0%
CAAGAAGATAGCAATAATGG+GGG - chr1_4:60722921-60722940 Msa0174950:CDS 35.0%
! AGTAGCATCAAAACTTGTTC+GGG - chr1_4:60722978-60722997 Msa0174950:intron 35.0%
! ATCAAAACTTGTTCGGGAAT+TGG - chr1_4:60722984-60723003 Msa0174950:intron 35.0%
! TCAAAACTTGTTCGGGAATT+GGG - chr1_4:60722985-60723004 Msa0174950:intron 35.0%
TGAAGAGAATGAAGCCAAAT+TGG - chr1_4:60723050-60723069 Msa0174950:intron 35.0%
ATTGTCTGAGTCCAAATACT+TGG - chr1_4:60723101-60723120 Msa0174950:intron 35.0%
TTGTCTGAGTCCAAATACTT+GGG - chr1_4:60723102-60723121 Msa0174950:intron 35.0%
TAAATGATGCAAATCCACCA+AGG + chr1_4:60723142-60723161 None:intergenic 35.0%
CTTAATTAGGTATCATGGCA+AGG + chr1_4:60723275-60723294 None:intergenic 35.0%
GAATGAAGATTCTTCACTGA+AGG + chr1_4:60723404-60723423 None:intergenic 35.0%
! CGAATCAAAGACATTGGTTA+AGG - chr1_4:60723454-60723473 Msa0174950:CDS 35.0%
TCAATGTGGTTGGAACAAGA+AGG + chr1_4:60722500-60722519 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1_4:60722555-60722574 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1_4:60722561-60722580 Msa0174950:CDS 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1_4:60722902-60722921 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1_4:60722923-60722942 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1_4:60722926-60722945 Msa0174950:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1_4:60722966-60722985 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1_4:60722986-60723005 Msa0174950:intron 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1_4:60723212-60723231 Msa0174950:intron 40.0%
TTGGTTAAGGGATTTGAGTC+CGG - chr1_4:60723467-60723486 Msa0174950:CDS 40.0%
TCAATGTGGTTGGAACAAGA+AGG + chr1_4:60722500-60722519 None:intergenic 40.0%
AAAGCTGGAACTTGACCAAA+TGG + chr1_4:60722555-60722574 None:intergenic 40.0%
!! CAAGTTCCAGCTTTTGAAGA+TGG - chr1_4:60722561-60722580 Msa0174950:CDS 40.0%
! GGAGTCATTGCTTGTTAACT+TGG + chr1_4:60722902-60722921 None:intergenic 40.0%
CCCCATTATTGCTATCTTCT+TGG + chr1_4:60722923-60722942 None:intergenic 40.0%
! AGATAGCAATAATGGGGGTA+TGG - chr1_4:60722926-60722945 Msa0174950:CDS 40.0%
! ATGCTACTTGATCGAAGTGT+TGG + chr1_4:60722966-60722985 None:intergenic 40.0%
! CAAAACTTGTTCGGGAATTG+GGG - chr1_4:60722986-60723005 Msa0174950:intron 40.0%
ACGATCTCATGTTAGTGCTT+GGG - chr1_4:60723212-60723231 Msa0174950:intron 40.0%
TTGGTTAAGGGATTTGAGTC+CGG - chr1_4:60723467-60723486 Msa0174950:CDS 40.0%
CTAATATTTGTGCGAGCGCT+TGG - chr1_4:60722781-60722800 Msa0174950:CDS 45.0%
TCTGAGTCCAAATACTTGGG+AGG - chr1_4:60723105-60723124 Msa0174950:intron 45.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1_4:60723115-60723134 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1_4:60723211-60723230 Msa0174950:intron 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1_4:60723215-60723234 Msa0174950:CDS 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1_4:60723241-60723260 Msa0174950:CDS 45.0%
CTAATATTTGTGCGAGCGCT+TGG - chr1_4:60722781-60722800 Msa0174950:CDS 45.0%
TCTGAGTCCAAATACTTGGG+AGG - chr1_4:60723105-60723124 Msa0174950:intron 45.0%
AGAGTCACCTCCCAAGTATT+TGG + chr1_4:60723115-60723134 None:intergenic 45.0%
CACGATCTCATGTTAGTGCT+TGG - chr1_4:60723211-60723230 Msa0174950:intron 45.0%
ATCTCATGTTAGTGCTTGGG+TGG - chr1_4:60723215-60723234 Msa0174950:CDS 45.0%
ATATCACTGCAAGACCAGCT+TGG - chr1_4:60723241-60723260 Msa0174950:CDS 45.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1_4:60722936-60722955 Msa0174950:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1_4:60723125-60723144 Msa0174950:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1_4:60723258-60723277 None:intergenic 50.0%
! AATGGGGGTATGGTCTGAAG+TGG - chr1_4:60722936-60722955 Msa0174950:CDS 50.0%
AGGTGACTCTTTCACCTTGG+TGG - chr1_4:60723125-60723144 Msa0174950:intron 50.0%
GCAAGGACTTTAGACCAAGC+TGG + chr1_4:60723258-60723277 None:intergenic 50.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1_4:60722252-60722271 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG + chr1_4:60722255-60722274 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1_4:60723122-60723141 Msa0174950:intron 55.0%
AGTTGCAGTAACCCGCATGG+TGG + chr1_4:60722252-60722271 None:intergenic 55.0%
CAGAGTTGCAGTAACCCGCA+TGG + chr1_4:60722255-60722274 None:intergenic 55.0%
GGGAGGTGACTCTTTCACCT+TGG - chr1_4:60723122-60723141 Msa0174950:intron 55.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1_4:60722226-60722245 Msa0174950:three_prime_UTR 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1_4:60722238-60722257 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1_4:60722239-60722258 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG - chr1_4:60722237-60722256 Msa0174950:three_prime_UTR 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1_4:60722238-60722257 Msa0174950:three_prime_UTR 60.0%
GCACGGAAGCCCCTACTCAA+CGG - chr1_4:60722226-60722245 Msa0174950:three_prime_UTR 60.0%
GCATGGTGGCCGTTGAGTAG+GGG + chr1_4:60722238-60722257 None:intergenic 60.0%
CGCATGGTGGCCGTTGAGTA+GGG + chr1_4:60722239-60722258 None:intergenic 60.0%
CCTACTCAACGGCCACCATG+CGG - chr1_4:60722237-60722256 Msa0174950:three_prime_UTR 60.0%
CTACTCAACGGCCACCATGC+GGG - chr1_4:60722238-60722257 Msa0174950:three_prime_UTR 60.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1_4:60722240-60722259 None:intergenic 65.0%
CCGCATGGTGGCCGTTGAGT+AGG + chr1_4:60722240-60722259 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60722152 60723661 60722152 ID=Msa0174950;Name=Msa0174950
chr1_4 mRNA 60722152 60723661 60722152 ID=Msa0174950-mRNA-1;Parent=Msa0174950;Name=Msa0174950-mRNA-1;_AED=0.21;_eAED=0.21;_QI=124|1|1|1|1|1|3|368|192
chr1_4 exon 60722152 60722965 60722152 ID=Msa0174950-mRNA-1:exon:19288;Parent=Msa0174950-mRNA-1
chr1_4 exon 60723213 60723261 60723213 ID=Msa0174950-mRNA-1:exon:19287;Parent=Msa0174950-mRNA-1
chr1_4 exon 60723454 60723661 60723454 ID=Msa0174950-mRNA-1:exon:19286;Parent=Msa0174950-mRNA-1
chr1_4 five_prime_UTR 60723538 60723661 60723538 ID=Msa0174950-mRNA-1:five_prime_utr;Parent=Msa0174950-mRNA-1
chr1_4 CDS 60723454 60723537 60723454 ID=Msa0174950-mRNA-1:cds;Parent=Msa0174950-mRNA-1
chr1_4 CDS 60723213 60723261 60723213 ID=Msa0174950-mRNA-1:cds;Parent=Msa0174950-mRNA-1
chr1_4 CDS 60722520 60722965 60722520 ID=Msa0174950-mRNA-1:cds;Parent=Msa0174950-mRNA-1
chr1_4 three_prime_UTR 60722152 60722519 60722152 ID=Msa0174950-mRNA-1:three_prime_utr;Parent=Msa0174950-mRNA-1
Gene Sequence

>Msa0174950

ATGAAAAACAGATTTGAGTTTGAGTTTGTTAACATTAACTTGAAGAATGGAGAACACAAAAAGGAACCCTTCATATCACTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGATGGAGATTTAAAGCTCTTCGAATCAAGGGCGATAACACAATATATTAACAATGAGTATGCTGATAAGGGGACCAAGTTAACAAGCAATGACTCCAAGAAGATAGCAATAATGGGGGTATGGTCTGAAGTGGAGAGCCAACACTTCGATCAAGTAGCATCAAAACTTGTTCGGGAATTGGGGATAAAGAGTTTATTTGGAATTCCTTTGGATCCAAAAATTGTTGAAGAGAATGAAGCCAAATTGGATAGCATTCTTGATGTGTATGAGAAAAGATTGTCTGAGTCCAAATACTTGGGAGGTGACTCTTTCACCTTGGTGGATTTGCATCATTTACCTACTTTTCATTACTTGATGAAGTCACAGACCAAGAAATTGTTTGAATCACGATCTCATGTTAGTGCTTGGGTGGCTGATATCACTGCAAGACCAGCTTGGTCTAAAGTCCTTGCCATGATACCTAATTAA
Protein sequence

>Msa0174950

MKNRFEFEFVNINLKNGEHKKEPFISLNPFGQVPAFEDGDLKLFESRAITQYINNEYADKGTKLTSNDSKKIAIMGVWSEVESQHFDQVASKLVRELGIKSLFGIPLDPKIVEENEAKLDSILDVYEKRLSESKYLGGDSFTLVDLHHLPTFHYLMKSQTKKLFESRSHVSAWVADITARPAWSKVLAMIPN*