AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGGATTCTACTGTTAA+AGG 0.187776 1_3:+68193638 Msa0136370:three_prime_UTR
ATAACCACTCCATTTGTTTC+AGG 0.253008 1_3:-68193189 None:intergenic
GTTAAAGGTGAAGGTGGTTT+TGG 0.282116 1_3:+68193653 Msa0136370:three_prime_UTR
ATTAAGGAATTTGGGTGATT+TGG 0.293926 1_3:+68193844 Msa0136370:three_prime_UTR
TTTGCTGTATTAAGGAATTT+GGG 0.349595 1_3:+68193836 Msa0136370:three_prime_UTR
TGGAAGGTGTTTGCATGGTT+TGG 0.363483 1_3:+68193871 Msa0136370:three_prime_UTR
CTGATTACAAAGGACTTGTT+GGG 0.366358 1_3:+68193507 Msa0136370:three_prime_UTR
ACACGAGCATAGGTTCCTTC+AGG 0.369361 1_3:-68193324 None:intergenic
CGCCGCCGATTCAAAGGTTC+CGG 0.372421 1_3:+68193257 Msa0136370:CDS
AACCTTTGAATCGGCGGCGC+TGG 0.377340 1_3:-68193253 None:intergenic
AATTTGGGTGATTTGGTTAG+TGG 0.377664 1_3:+68193851 Msa0136370:three_prime_UTR
CTCAATAATATTCTCAGAAT+AGG 0.417003 1_3:-68193776 None:intergenic
GCTGATTACAAAGGACTTGT+TGG 0.453307 1_3:+68193506 Msa0136370:three_prime_UTR
CATTCGATTGACGTTGGTGT+TGG 0.453984 1_3:+68193473 Msa0136370:three_prime_UTR
CTTAATACAGCAAAATAGTA+AGG 0.468503 1_3:-68193827 None:intergenic
AAGAAGTAACCTGAAACAAA+TGG 0.471031 1_3:+68193180 Msa0136370:exon
CGCCGAGATCGGGGTTGACA+TGG 0.473074 1_3:+68193447 Msa0136370:exon
TATTGAGAAGATTATTACTC+TGG 0.475325 1_3:+68193598 Msa0136370:three_prime_UTR
TTCCATGTCAACCCCGATCT+CGG 0.481839 1_3:-68193449 None:intergenic
ATGAGAGCTTTGCCGCGAGC+CGG 0.494102 1_3:-68193276 None:intergenic
CTTCAAATTGATGAGCTGAA+AGG 0.505766 1_3:-68193160 None:intergenic
GATCGTATCCGACGGAGAGA+GGG 0.507298 1_3:-68193227 None:intergenic
CTCCAGCGCCGCCGATTCAA+AGG 0.509909 1_3:+68193251 Msa0136370:CDS
CTCTCCATCGCCGTCCCTGA+AGG 0.513836 1_3:+68193309 Msa0136370:CDS
CGCCAAACTCATCTCTCTCT+AGG 0.517717 1_3:+68193090 None:intergenic
GCGAGCCGGAACCTTTGAAT+CGG 0.523716 1_3:-68193262 None:intergenic
ATGAGCTGAAAGGAAGGGAA+TGG 0.528729 1_3:-68193150 None:intergenic
CGCGGCTCCCCTCTCTCCGT+CGG 0.534180 1_3:+68193219 Msa0136370:CDS
CACGAGCATAGGTTCCTTCA+GGG 0.535140 1_3:-68193323 None:intergenic
TTTAACAGTGTGTGTAGTTG+GGG 0.539552 1_3:+68193716 Msa0136370:three_prime_UTR
AGGAAGGGAATGGAAGAGTG+TGG 0.541214 1_3:-68193140 None:intergenic
GATTCTACTGTTAAAGGTGA+AGG 0.543367 1_3:+68193644 Msa0136370:three_prime_UTR
TTAATTACCAACACGAGCAT+AGG 0.548576 1_3:-68193334 None:intergenic
GAGAGAGGGGAGCCGCGAGA+AGG 0.552420 1_3:-68193213 None:intergenic
GTTAGTGGAAGGTGTTTGCA+TGG 0.552538 1_3:+68193866 Msa0136370:three_prime_UTR
ACGGCGATGGAGAGATCTGT+GGG 0.554559 1_3:-68193300 None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG 0.559917 1_3:-68193313 None:intergenic
AAATTGATGAGCTGAAAGGA+AGG 0.560626 1_3:-68193156 None:intergenic
TCTACTGTTAAAGGTGAAGG+TGG 0.564985 1_3:+68193647 Msa0136370:three_prime_UTR
AGCCGGAACCTTTGAATCGG+CGG 0.570711 1_3:-68193259 None:intergenic
ATCGTATCCGACGGAGAGAG+GGG 0.576335 1_3:-68193226 None:intergenic
AAGAGTGTGGAAGATGAAGA+TGG 0.578389 1_3:-68193127 None:intergenic
GACGGCGATGGAGAGATCTG+TGG 0.578622 1_3:-68193301 None:intergenic
GATTCAAAGGTTCCGGCTCG+CGG 0.589951 1_3:+68193264 Msa0136370:CDS
TGGGTGATTTGGTTAGTGGA+AGG 0.594970 1_3:+68193855 Msa0136370:three_prime_UTR
AGATCGTATCCGACGGAGAG+AGG 0.603566 1_3:-68193228 None:intergenic
AGAGTGTGGAAGATGAAGAT+GGG 0.603613 1_3:-68193126 None:intergenic
GAAGGAACCTATGCTCGTGT+TGG 0.606547 1_3:+68193327 Msa0136370:CDS
AATTGATGAGCTGAAAGGAA+GGG 0.609408 1_3:-68193155 None:intergenic
TGATTACAAAGGACTTGTTG+GGG 0.614182 1_3:+68193508 Msa0136370:three_prime_UTR
AGCATAGGTTCCTTCAGGGA+CGG 0.629807 1_3:-68193319 None:intergenic
TGGAAGCATTCGATTGACGT+TGG 0.634202 1_3:+68193467 Msa0136370:three_prime_UTR
GTAACCTGAAACAAATGGAG+TGG 0.654678 1_3:+68193185 Msa0136370:exon
GGAGTGGTTATACCTTCTCG+CGG 0.672235 1_3:+68193201 Msa0136370:CDS
GTGATTGATGCTGATTACAA+AGG 0.678416 1_3:+68193497 Msa0136370:three_prime_UTR
GCTGGAGAGATCGTATCCGA+CGG 0.689217 1_3:-68193235 None:intergenic
GTGTGGAAGATGAAGATGGG+TGG 0.708700 1_3:-68193123 None:intergenic
CGGCGATGGAGAGATCTGTG+GGG 0.756952 1_3:-68193299 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGGGTTTTTGAAATTT+AGG + chr1_3:68193394-68193413 Msa0136370:intron 20.0%
!!! TTTAGGGTTTTTGAAATTTA+GGG + chr1_3:68193395-68193414 Msa0136370:intron 20.0%
!!! TTTTAGGGTTTTTGAAATTT+AGG + chr1_3:68193394-68193413 Msa0136370:intron 20.0%
!!! TTTAGGGTTTTTGAAATTTA+GGG + chr1_3:68193395-68193414 Msa0136370:intron 20.0%
! AAAAACCTGAAATTGGAAAA+GGG - chr1_3:68193371-68193390 None:intergenic 25.0%
! AAAAGTCAAAAACCTGAAAT+TGG - chr1_3:68193378-68193397 None:intergenic 25.0%
!!! ATTTCAGGTTTTTGACTTTT+AGG + chr1_3:68193378-68193397 Msa0136370:intron 25.0%
!!! TTTCAGGTTTTTGACTTTTA+GGG + chr1_3:68193379-68193398 Msa0136370:intron 25.0%
! TATTGAGAAGATTATTACTC+TGG + chr1_3:68193598-68193617 Msa0136370:three_prime_UTR 25.0%
!!! TTTACTATTTTGCTGTGTTA+AGG + chr1_3:68193751-68193770 Msa0136370:three_prime_UTR 25.0%
! CTCAATAATATTCTCAGAAT+AGG - chr1_3:68193779-68193798 None:intergenic 25.0%
! CTTAATACAGCAAAATAGTA+AGG - chr1_3:68193830-68193849 None:intergenic 25.0%
!!! CTTACTATTTTGCTGTATTA+AGG + chr1_3:68193828-68193847 Msa0136370:three_prime_UTR 25.0%
!!! TTTTGCTGTATTAAGGAATT+TGG + chr1_3:68193835-68193854 Msa0136370:three_prime_UTR 25.0%
!! TTTGCTGTATTAAGGAATTT+GGG + chr1_3:68193836-68193855 Msa0136370:three_prime_UTR 25.0%
! AAAAACCTGAAATTGGAAAA+GGG - chr1_3:68193371-68193390 None:intergenic 25.0%
! AAAAGTCAAAAACCTGAAAT+TGG - chr1_3:68193378-68193397 None:intergenic 25.0%
!!! ATTTCAGGTTTTTGACTTTT+AGG + chr1_3:68193378-68193397 Msa0136370:intron 25.0%
!!! TTTCAGGTTTTTGACTTTTA+GGG + chr1_3:68193379-68193398 Msa0136370:intron 25.0%
! TATTGAGAAGATTATTACTC+TGG + chr1_3:68193598-68193617 Msa0136370:three_prime_UTR 25.0%
!!! TTTACTATTTTGCTGTGTTA+AGG + chr1_3:68193751-68193770 Msa0136370:three_prime_UTR 25.0%
! CTCAATAATATTCTCAGAAT+AGG - chr1_3:68193779-68193798 None:intergenic 25.0%
! CTTAATACAGCAAAATAGTA+AGG - chr1_3:68193830-68193849 None:intergenic 25.0%
!!! CTTACTATTTTGCTGTATTA+AGG + chr1_3:68193828-68193847 Msa0136370:three_prime_UTR 25.0%
!!! TTTTGCTGTATTAAGGAATT+TGG + chr1_3:68193835-68193854 Msa0136370:three_prime_UTR 25.0%
!! TTTGCTGTATTAAGGAATTT+GGG + chr1_3:68193836-68193855 Msa0136370:three_prime_UTR 25.0%
AAGAAGTAACCTGAAACAAA+TGG + chr1_3:68193180-68193199 Msa0136370:exon 30.0%
AAAACCTGAAATTGGAAAAG+GGG - chr1_3:68193370-68193389 None:intergenic 30.0%
CAAAAACCTGAAATTGGAAA+AGG - chr1_3:68193372-68193391 None:intergenic 30.0%
!!! GTTGATTTTGAAGTGAAAGT+TGG + chr1_3:68193554-68193573 Msa0136370:three_prime_UTR 30.0%
GATTTGGATTCTACTGTTAA+AGG + chr1_3:68193638-68193657 Msa0136370:three_prime_UTR 30.0%
!! ATTTTAACAGTGTGTGTAGT+TGG + chr1_3:68193714-68193733 Msa0136370:three_prime_UTR 30.0%
! TTTTAACAGTGTGTGTAGTT+GGG + chr1_3:68193715-68193734 Msa0136370:three_prime_UTR 30.0%
ATTAAGGAATTTGGGTGATT+TGG + chr1_3:68193844-68193863 Msa0136370:three_prime_UTR 30.0%
AAGAAGTAACCTGAAACAAA+TGG + chr1_3:68193180-68193199 Msa0136370:exon 30.0%
AAAACCTGAAATTGGAAAAG+GGG - chr1_3:68193370-68193389 None:intergenic 30.0%
CAAAAACCTGAAATTGGAAA+AGG - chr1_3:68193372-68193391 None:intergenic 30.0%
!!! GTTGATTTTGAAGTGAAAGT+TGG + chr1_3:68193554-68193573 Msa0136370:three_prime_UTR 30.0%
GATTTGGATTCTACTGTTAA+AGG + chr1_3:68193638-68193657 Msa0136370:three_prime_UTR 30.0%
!! ATTTTAACAGTGTGTGTAGT+TGG + chr1_3:68193714-68193733 Msa0136370:three_prime_UTR 30.0%
! TTTTAACAGTGTGTGTAGTT+GGG + chr1_3:68193715-68193734 Msa0136370:three_prime_UTR 30.0%
ATTAAGGAATTTGGGTGATT+TGG + chr1_3:68193844-68193863 Msa0136370:three_prime_UTR 30.0%
AATTGATGAGCTGAAAGGAA+GGG - chr1_3:68193158-68193177 None:intergenic 35.0%
AAATTGATGAGCTGAAAGGA+AGG - chr1_3:68193159-68193178 None:intergenic 35.0%
CTTCAAATTGATGAGCTGAA+AGG - chr1_3:68193163-68193182 None:intergenic 35.0%
ATAACCACTCCATTTGTTTC+AGG - chr1_3:68193192-68193211 None:intergenic 35.0%
TTAATTACCAACACGAGCAT+AGG - chr1_3:68193337-68193356 None:intergenic 35.0%
! TTTTCCCCTTTTCCAATTTC+AGG + chr1_3:68193363-68193382 Msa0136370:intron 35.0%
! GTGATTGATGCTGATTACAA+AGG + chr1_3:68193497-68193516 Msa0136370:three_prime_UTR 35.0%
! CTGATTACAAAGGACTTGTT+GGG + chr1_3:68193507-68193526 Msa0136370:three_prime_UTR 35.0%
!! TGATTACAAAGGACTTGTTG+GGG + chr1_3:68193508-68193527 Msa0136370:three_prime_UTR 35.0%
!! TATTACTCTGGAAGTTTTCG+AGG + chr1_3:68193610-68193629 Msa0136370:three_prime_UTR 35.0%
!!! AGTTTTCGAGGTTTTGGATT+TGG + chr1_3:68193622-68193641 Msa0136370:three_prime_UTR 35.0%
GATTCTACTGTTAAAGGTGA+AGG + chr1_3:68193644-68193663 Msa0136370:three_prime_UTR 35.0%
TTTAACAGTGTGTGTAGTTG+GGG + chr1_3:68193716-68193735 Msa0136370:three_prime_UTR 35.0%
!! AATTTGGGTGATTTGGTTAG+TGG + chr1_3:68193851-68193870 Msa0136370:three_prime_UTR 35.0%
AATTGATGAGCTGAAAGGAA+GGG - chr1_3:68193158-68193177 None:intergenic 35.0%
AAATTGATGAGCTGAAAGGA+AGG - chr1_3:68193159-68193178 None:intergenic 35.0%
CTTCAAATTGATGAGCTGAA+AGG - chr1_3:68193163-68193182 None:intergenic 35.0%
ATAACCACTCCATTTGTTTC+AGG - chr1_3:68193192-68193211 None:intergenic 35.0%
TTAATTACCAACACGAGCAT+AGG - chr1_3:68193337-68193356 None:intergenic 35.0%
! TTTTCCCCTTTTCCAATTTC+AGG + chr1_3:68193363-68193382 Msa0136370:intron 35.0%
! GTGATTGATGCTGATTACAA+AGG + chr1_3:68193497-68193516 Msa0136370:three_prime_UTR 35.0%
! CTGATTACAAAGGACTTGTT+GGG + chr1_3:68193507-68193526 Msa0136370:three_prime_UTR 35.0%
!! TGATTACAAAGGACTTGTTG+GGG + chr1_3:68193508-68193527 Msa0136370:three_prime_UTR 35.0%
!! TATTACTCTGGAAGTTTTCG+AGG + chr1_3:68193610-68193629 Msa0136370:three_prime_UTR 35.0%
!!! AGTTTTCGAGGTTTTGGATT+TGG + chr1_3:68193622-68193641 Msa0136370:three_prime_UTR 35.0%
GATTCTACTGTTAAAGGTGA+AGG + chr1_3:68193644-68193663 Msa0136370:three_prime_UTR 35.0%
TTTAACAGTGTGTGTAGTTG+GGG + chr1_3:68193716-68193735 Msa0136370:three_prime_UTR 35.0%
!! AATTTGGGTGATTTGGTTAG+TGG + chr1_3:68193851-68193870 Msa0136370:three_prime_UTR 35.0%
AGAGTGTGGAAGATGAAGAT+GGG - chr1_3:68193129-68193148 None:intergenic 40.0%
! AAGAGTGTGGAAGATGAAGA+TGG - chr1_3:68193130-68193149 None:intergenic 40.0%
GTAACCTGAAACAAATGGAG+TGG + chr1_3:68193185-68193204 Msa0136370:exon 40.0%
GCTGATTACAAAGGACTTGT+TGG + chr1_3:68193506-68193525 Msa0136370:three_prime_UTR 40.0%
!! TCTGGAAGTTTTCGAGGTTT+TGG + chr1_3:68193616-68193635 Msa0136370:three_prime_UTR 40.0%
TCTACTGTTAAAGGTGAAGG+TGG + chr1_3:68193647-68193666 Msa0136370:three_prime_UTR 40.0%
! GTTAAAGGTGAAGGTGGTTT+TGG + chr1_3:68193653-68193672 Msa0136370:three_prime_UTR 40.0%
!!! AAGGTGGTTTTGGATCTACT+GGG + chr1_3:68193663-68193682 Msa0136370:three_prime_UTR 40.0%
AGAGTGTGGAAGATGAAGAT+GGG - chr1_3:68193129-68193148 None:intergenic 40.0%
! AAGAGTGTGGAAGATGAAGA+TGG - chr1_3:68193130-68193149 None:intergenic 40.0%
GTAACCTGAAACAAATGGAG+TGG + chr1_3:68193185-68193204 Msa0136370:exon 40.0%
GCTGATTACAAAGGACTTGT+TGG + chr1_3:68193506-68193525 Msa0136370:three_prime_UTR 40.0%
!! TCTGGAAGTTTTCGAGGTTT+TGG + chr1_3:68193616-68193635 Msa0136370:three_prime_UTR 40.0%
TCTACTGTTAAAGGTGAAGG+TGG + chr1_3:68193647-68193666 Msa0136370:three_prime_UTR 40.0%
! GTTAAAGGTGAAGGTGGTTT+TGG + chr1_3:68193653-68193672 Msa0136370:three_prime_UTR 40.0%
!!! AAGGTGGTTTTGGATCTACT+GGG + chr1_3:68193663-68193682 Msa0136370:three_prime_UTR 40.0%
ATGAGCTGAAAGGAAGGGAA+TGG - chr1_3:68193153-68193172 None:intergenic 45.0%
!! TGGAAGCATTCGATTGACGT+TGG + chr1_3:68193467-68193486 Msa0136370:three_prime_UTR 45.0%
CATTCGATTGACGTTGGTGT+TGG + chr1_3:68193473-68193492 Msa0136370:three_prime_UTR 45.0%
!!! GAAGGTGGTTTTGGATCTAC+TGG + chr1_3:68193662-68193681 Msa0136370:three_prime_UTR 45.0%
!!! AGGTGGTTTTGGATCTACTG+GGG + chr1_3:68193664-68193683 Msa0136370:three_prime_UTR 45.0%
!! TGGGTGATTTGGTTAGTGGA+AGG + chr1_3:68193855-68193874 Msa0136370:three_prime_UTR 45.0%
!! GTTAGTGGAAGGTGTTTGCA+TGG + chr1_3:68193866-68193885 Msa0136370:three_prime_UTR 45.0%
ATGAGCTGAAAGGAAGGGAA+TGG - chr1_3:68193153-68193172 None:intergenic 45.0%
!! TGGAAGCATTCGATTGACGT+TGG + chr1_3:68193467-68193486 Msa0136370:three_prime_UTR 45.0%
CATTCGATTGACGTTGGTGT+TGG + chr1_3:68193473-68193492 Msa0136370:three_prime_UTR 45.0%
!!! GAAGGTGGTTTTGGATCTAC+TGG + chr1_3:68193662-68193681 Msa0136370:three_prime_UTR 45.0%
!!! AGGTGGTTTTGGATCTACTG+GGG + chr1_3:68193664-68193683 Msa0136370:three_prime_UTR 45.0%
!! TGGGTGATTTGGTTAGTGGA+AGG + chr1_3:68193855-68193874 Msa0136370:three_prime_UTR 45.0%
!! GTTAGTGGAAGGTGTTTGCA+TGG + chr1_3:68193866-68193885 Msa0136370:three_prime_UTR 45.0%
GTGTGGAAGATGAAGATGGG+TGG - chr1_3:68193126-68193145 None:intergenic 50.0%
AGGAAGGGAATGGAAGAGTG+TGG - chr1_3:68193143-68193162 None:intergenic 50.0%
GGAGTGGTTATACCTTCTCG+CGG + chr1_3:68193201-68193220 Msa0136370:CDS 50.0%
AGCATAGGTTCCTTCAGGGA+CGG - chr1_3:68193322-68193341 None:intergenic 50.0%
CACGAGCATAGGTTCCTTCA+GGG - chr1_3:68193326-68193345 None:intergenic 50.0%
ACACGAGCATAGGTTCCTTC+AGG - chr1_3:68193327-68193346 None:intergenic 50.0%
GAAGGAACCTATGCTCGTGT+TGG + chr1_3:68193327-68193346 Msa0136370:CDS 50.0%
!! GATTTTTGCAGCGCCGAGAT+CGG + chr1_3:68193436-68193455 Msa0136370:intron 50.0%
!! ATTTTTGCAGCGCCGAGATC+GGG + chr1_3:68193437-68193456 Msa0136370:intron 50.0%
TTCCATGTCAACCCCGATCT+CGG - chr1_3:68193452-68193471 None:intergenic 50.0%
GTGTGGAAGATGAAGATGGG+TGG - chr1_3:68193126-68193145 None:intergenic 50.0%
AGGAAGGGAATGGAAGAGTG+TGG - chr1_3:68193143-68193162 None:intergenic 50.0%
GGAGTGGTTATACCTTCTCG+CGG + chr1_3:68193201-68193220 Msa0136370:CDS 50.0%
AGCATAGGTTCCTTCAGGGA+CGG - chr1_3:68193322-68193341 None:intergenic 50.0%
CACGAGCATAGGTTCCTTCA+GGG - chr1_3:68193326-68193345 None:intergenic 50.0%
ACACGAGCATAGGTTCCTTC+AGG - chr1_3:68193327-68193346 None:intergenic 50.0%
GAAGGAACCTATGCTCGTGT+TGG + chr1_3:68193327-68193346 Msa0136370:CDS 50.0%
!! GATTTTTGCAGCGCCGAGAT+CGG + chr1_3:68193436-68193455 Msa0136370:intron 50.0%
!! ATTTTTGCAGCGCCGAGATC+GGG + chr1_3:68193437-68193456 Msa0136370:intron 50.0%
TTCCATGTCAACCCCGATCT+CGG - chr1_3:68193452-68193471 None:intergenic 50.0%
ATCGTATCCGACGGAGAGAG+GGG - chr1_3:68193229-68193248 None:intergenic 55.0%
GATCGTATCCGACGGAGAGA+GGG - chr1_3:68193230-68193249 None:intergenic 55.0%
AGATCGTATCCGACGGAGAG+AGG - chr1_3:68193231-68193250 None:intergenic 55.0%
GCTGGAGAGATCGTATCCGA+CGG - chr1_3:68193238-68193257 None:intergenic 55.0%
AGCCGGAACCTTTGAATCGG+CGG - chr1_3:68193262-68193281 None:intergenic 55.0%
GCGAGCCGGAACCTTTGAAT+CGG - chr1_3:68193265-68193284 None:intergenic 55.0%
! GATTCAAAGGTTCCGGCTCG+CGG + chr1_3:68193264-68193283 Msa0136370:CDS 55.0%
ACGGCGATGGAGAGATCTGT+GGG - chr1_3:68193303-68193322 None:intergenic 55.0%
!! TTTTTGCAGCGCCGAGATCG+GGG + chr1_3:68193438-68193457 Msa0136370:intron 55.0%
ATCGTATCCGACGGAGAGAG+GGG - chr1_3:68193229-68193248 None:intergenic 55.0%
GATCGTATCCGACGGAGAGA+GGG - chr1_3:68193230-68193249 None:intergenic 55.0%
AGATCGTATCCGACGGAGAG+AGG - chr1_3:68193231-68193250 None:intergenic 55.0%
GCTGGAGAGATCGTATCCGA+CGG - chr1_3:68193238-68193257 None:intergenic 55.0%
AGCCGGAACCTTTGAATCGG+CGG - chr1_3:68193262-68193281 None:intergenic 55.0%
GCGAGCCGGAACCTTTGAAT+CGG - chr1_3:68193265-68193284 None:intergenic 55.0%
! GATTCAAAGGTTCCGGCTCG+CGG + chr1_3:68193264-68193283 Msa0136370:CDS 55.0%
ACGGCGATGGAGAGATCTGT+GGG - chr1_3:68193303-68193322 None:intergenic 55.0%
!! TTTTTGCAGCGCCGAGATCG+GGG + chr1_3:68193438-68193457 Msa0136370:intron 55.0%
AACCTTTGAATCGGCGGCGC+TGG - chr1_3:68193256-68193275 None:intergenic 60.0%
!! CGCCGCCGATTCAAAGGTTC+CGG + chr1_3:68193257-68193276 Msa0136370:CDS 60.0%
ATGAGAGCTTTGCCGCGAGC+CGG - chr1_3:68193279-68193298 None:intergenic 60.0%
CGGCGATGGAGAGATCTGTG+GGG - chr1_3:68193302-68193321 None:intergenic 60.0%
GACGGCGATGGAGAGATCTG+TGG - chr1_3:68193304-68193323 None:intergenic 60.0%
AACCTTTGAATCGGCGGCGC+TGG - chr1_3:68193256-68193275 None:intergenic 60.0%
!! CGCCGCCGATTCAAAGGTTC+CGG + chr1_3:68193257-68193276 Msa0136370:CDS 60.0%
ATGAGAGCTTTGCCGCGAGC+CGG - chr1_3:68193279-68193298 None:intergenic 60.0%
CGGCGATGGAGAGATCTGTG+GGG - chr1_3:68193302-68193321 None:intergenic 60.0%
GACGGCGATGGAGAGATCTG+TGG - chr1_3:68193304-68193323 None:intergenic 60.0%
!! CTCCAGCGCCGCCGATTCAA+AGG + chr1_3:68193251-68193270 Msa0136370:CDS 65.0%
CTCTCCATCGCCGTCCCTGA+AGG + chr1_3:68193309-68193328 Msa0136370:CDS 65.0%
GGTTCCTTCAGGGACGGCGA+TGG - chr1_3:68193316-68193335 None:intergenic 65.0%
CGCCGAGATCGGGGTTGACA+TGG + chr1_3:68193447-68193466 Msa0136370:exon 65.0%
!! CTCCAGCGCCGCCGATTCAA+AGG + chr1_3:68193251-68193270 Msa0136370:CDS 65.0%
CTCTCCATCGCCGTCCCTGA+AGG + chr1_3:68193309-68193328 Msa0136370:CDS 65.0%
GGTTCCTTCAGGGACGGCGA+TGG - chr1_3:68193316-68193335 None:intergenic 65.0%
CGCCGAGATCGGGGTTGACA+TGG + chr1_3:68193447-68193466 Msa0136370:exon 65.0%
GAGAGAGGGGAGCCGCGAGA+AGG - chr1_3:68193216-68193235 None:intergenic 70.0%
GAGAGAGGGGAGCCGCGAGA+AGG - chr1_3:68193216-68193235 None:intergenic 70.0%
CGCGGCTCCCCTCTCTCCGT+CGG + chr1_3:68193219-68193238 Msa0136370:CDS 75.0%
CGCGGCTCCCCTCTCTCCGT+CGG + chr1_3:68193219-68193238 Msa0136370:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 68193097 68193892 68193097 ID=Msa0136370;Name=Msa0136370
chr1_3 mRNA 68193097 68193892 68193097 ID=Msa0136370-mRNA-1;Parent=Msa0136370;Name=Msa0136370-mRNA-1;_AED=0.00;_eAED=0.00;_QI=102|1|1|1|1|1|2|428|55
chr1_3 exon 68193097 68193348 68193097 ID=Msa0136370-mRNA-1:exon:23540;Parent=Msa0136370-mRNA-1
chr1_3 exon 68193447 68193892 68193447 ID=Msa0136370-mRNA-1:exon:23541;Parent=Msa0136370-mRNA-1
chr1_3 five_prime_UTR 68193097 68193198 68193097 ID=Msa0136370-mRNA-1:five_prime_utr;Parent=Msa0136370-mRNA-1
chr1_3 CDS 68193199 68193348 68193199 ID=Msa0136370-mRNA-1:cds;Parent=Msa0136370-mRNA-1
chr1_3 CDS 68193447 68193464 68193447 ID=Msa0136370-mRNA-1:cds;Parent=Msa0136370-mRNA-1
chr1_3 three_prime_UTR 68193465 68193892 68193465 ID=Msa0136370-mRNA-1:three_prime_utr;Parent=Msa0136370-mRNA-1
Gene Sequence

>Msa0136370

ATGGAGTGGTTATACCTTCTCGCGGCTCCCCTCTCTCCGTCGGATACGATCTCTCCAGCGCCGCCGATTCAAAGGTTCCGGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGCGCCGAGATCGGGGTTGA
Protein sequence

>Msa0136370

MEWLYLLAAPLSPSDTISPAPPIQRFRLAAKLSSPQISPSPSLKEPMLVLRRDRG*