AlfalfaGEDB | Msa0152530

M. sativa cultivar zhongmu-4 / Msa0152530

KEGG: K11975
GO: GO:0000151 GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0010941 GO:0016020 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030162 GO:0030163 GO:0031090 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031624 GO:0031966 GO:0031967 GO:0031975 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0036211 GO:0042176 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0060255 GO:0060548 GO:0061136 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
PFAM: PF01485.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152530	KEH43917.1	84.545	330	28	1	1	330	1	307	0.0	567

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152530	sp\|F4ITM1\|RGSL1_ARATH	28.502	207	144	4	126	330	159	363	2.88e-25	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152530	A0A072VQ64	84.545	330	28	1	1	330	1	307	0.0	567

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0012560	Msa0152530	0.884781	1.429621e-71	-8.615850e-47
Msa0061390	Msa0152530	0.882808	7.632497e-71	-8.615850e-47
Msa0114990	Msa0152530	0.882752	8.005155e-71	-8.615850e-47
Msa0152530	Msa1428610	0.823821	1.126755e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152530	MtrunA17_Chr1g0206011	87.006	177	23	0	131	307	15	191	1.09e-113	327
Msa0152530	MtrunA17_Chr1g0206001	79.577	142	29	0	187	328	3	144	1.31e-86	257
Msa0152530	MtrunA17_Chr1g0206031	75.497	151	28	1	95	236	15	165	1.33e-76	232
Msa0152530	MtrunA17_Chr2g0301841	53.333	195	89	1	124	318	94	286	1.33e-75	234
Msa0152530	MtrunA17_Chr4g0031451	55.051	198	86	2	131	327	2	197	1.39e-74	228
Msa0152530	MtrunA17_Chr4g0031441	44.599	287	127	5	14	300	9	263	5.14e-74	230
Msa0152530	MtrunA17_Chr2g0301761	46.465	198	102	2	115	310	67	262	4.47e-65	206
Msa0152530	MtrunA17_Chr2g0301821	46.524	187	98	1	124	310	85	269	1.01e-61	198
Msa0152530	MtrunA17_Chr2g0301741	45.178	197	106	1	116	312	59	253	2.49e-60	195
Msa0152530	MtrunA17_Chr8g0344981	47.236	199	101	3	115	312	52	247	1.29e-58	190
Msa0152530	MtrunA17_Chr3g0103941	37.849	251	146	4	75	324	3	244	4.77e-55	180
Msa0152530	MtrunA17_Chr2g0301751	40.758	211	110	3	115	312	68	276	1.45e-53	177
Msa0152530	MtrunA17_Chr3g0103851	42.786	201	112	2	126	324	247	446	4.42e-53	181
Msa0152530	MtrunA17_Chr3g0103961	39.423	208	121	3	119	324	48	252	1.90e-50	168
Msa0152530	MtrunA17_Chr3g0103871	39.091	220	128	2	94	307	25	244	1.31e-49	166
Msa0152530	MtrunA17_Chr3g0103931	39.234	209	121	3	117	324	44	247	5.69e-49	164
Msa0152530	MtrunA17_Chr2g0301731	38.144	194	115	2	122	314	64	253	8.67e-48	162
Msa0152530	MtrunA17_Chr3g0103911	36.170	235	140	4	77	310	5	230	7.40e-47	158
Msa0152530	MtrunA17_Chr3g0103891	35.865	237	142	4	75	310	3	230	1.03e-46	158
Msa0152530	MtrunA17_Chr4g0031461	57.600	125	50	2	204	327	1	123	1.77e-46	153
Msa0152530	MtrunA17_Chr3g0104451	40.191	209	115	3	126	324	26	234	5.24e-46	156
Msa0152530	MtrunA17_Chr3g0104441	40.394	203	112	2	126	319	30	232	6.15e-44	152
Msa0152530	MtrunA17_Chr2g0291681	37.915	211	122	2	126	328	213	422	1.48e-42	154
Msa0152530	MtrunA17_Chr5g0425671	35.135	222	126	4	92	307	78	287	1.74e-40	144
Msa0152530	MtrunA17_Chr4g0029991	34.286	210	128	4	126	327	307	514	1.00e-35	135
Msa0152530	MtrunA17_Chr5g0434721	24.927	341	186	7	3	324	29	318	3.21e-33	125
Msa0152530	MtrunA17_Chr3g0103861	31.754	211	106	4	111	317	44	220	2.97e-29	112
Msa0152530	MtrunA17_Chr3g0103951	42.276	123	68	2	186	307	20	140	6.49e-26	100
Msa0152530	MtrunA17_Chr1g0206041	92.857	42	3	0	264	305	1	42	6.16e-23	90.9
Msa0152530	MtrunA17_Chr3g0100351	28.780	205	93	4	126	327	46	200	1.33e-21	90.9
Msa0152530	MtrunA17_Chr3g0100371	28.947	228	104	6	103	329	47	217	3.36e-19	85.1
Msa0152530	MtrunA17_Chr1g0164621	28.685	251	149	8	77	323	1215	1439	1.16e-17	84.3
Msa0152530	MtrunA17_Chr3g0082091	28.458	253	147	9	77	323	1213	1437	3.08e-17	82.8
Msa0152530	MtrunA17_Chr3g0110331	27.523	218	146	8	117	329	126	336	2.31e-15	77.0
Msa0152530	MtrunA17_Chr3g0123681	29.048	210	129	7	120	323	1522	1717	6.83e-15	75.9
Msa0152530	MtrunA17_Chr3g0103841	45.714	70	37	1	256	324	1	70	1.71e-14	67.8
Msa0152530	MtrunA17_Chr5g0394311	37.963	108	58	4	212	316	169	270	1.16e-13	70.9
Msa0152530	MtrunA17_Chr4g0038181	26.222	225	146	8	103	316	67	282	2.66e-13	70.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0152530	AT3G14250.1	36.364	319	157	7	15	325	18	298	1.54e-72	227
Msa0152530	AT3G53690.1	50.259	193	95	1	126	318	115	306	6.71e-71	223
Msa0152530	AT5G37560.1	35.223	247	150	6	84	324	137	379	3.92e-42	152
Msa0152530	AT4G19670.2	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.3	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.5	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.7	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.4	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.1	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT4G19670.6	37.917	240	130	5	102	327	183	417	5.74e-42	153
Msa0152530	AT3G45470.1	33.005	203	133	3	126	327	6	206	1.04e-34	126
Msa0152530	AT2G21420.1	36.500	200	118	4	126	321	187	381	2.51e-34	131
Msa0152530	AT5G60250.1	33.491	212	128	4	126	327	301	509	2.24e-32	127
Msa0152530	AT3G45580.1	32.039	206	134	4	126	327	173	376	9.75e-32	123
Msa0152530	AT2G25370.2	35.354	198	122	4	126	320	247	441	2.21e-30	121
Msa0152530	AT2G25370.1	35.354	198	122	4	126	320	304	498	3.81e-30	120
Msa0152530	AT3G45540.1	31.250	208	131	6	126	328	100	300	4.69e-30	117
Msa0152530	AT3G45540.2	31.250	208	131	6	126	328	71	271	5.09e-30	116
Msa0152530	AT2G25360.1	35.266	207	127	6	125	327	89	292	1.34e-29	116
Msa0152530	AT2G26135.1	30.870	230	141	7	102	325	135	352	1.23e-28	114
Msa0152530	AT2G26130.1	28.502	207	144	4	126	330	159	363	2.93e-26	108
Msa0152530	AT3G45555.1	26.961	204	139	5	106	306	2	198	1.06e-23	97.4
Msa0152530	AT3G45460.1	28.426	197	123	5	136	330	181	361	7.69e-22	95.5
Msa0152530	AT3G45480.1	29.798	198	119	6	134	327	165	346	6.26e-21	92.8
Msa0152530	AT3G43750.1	29.947	187	124	5	126	307	155	339	6.78e-21	92.4
Msa0152530	AT2G19610.2	30.348	201	131	4	126	318	203	402	2.99e-19	88.2
Msa0152530	AT5G10370.1	28.977	176	117	5	144	316	1580	1750	5.38e-17	82.8
Msa0152530	AT4G01020.1	27.411	197	123	6	144	326	1577	1767	3.06e-16	80.5
Msa0152530	AT3G27710.1	27.053	207	145	4	120	324	115	317	5.76e-14	73.2
Msa0152530	AT2G19610.1	27.513	189	129	3	126	307	203	390	8.72e-14	72.0
Msa0152530	AT3G45510.1	28.571	140	96	3	126	262	66	204	3.48e-13	68.9
Msa0152530	AT2G31770.1	28.037	214	137	8	124	329	126	330	8.40e-13	69.3
Msa0152530	AT3G45570.1	27.778	144	99	3	126	265	163	305	2.00e-11	64.3
Msa0152530	AT2G31510.3	27.751	209	134	10	124	325	63	261	3.15e-11	64.7
Msa0152530	AT2G31510.2	27.751	209	134	10	124	325	135	333	4.97e-11	63.9
Msa0152530	AT2G31510.1	27.751	209	134	10	124	325	135	333	5.16e-11	63.9

Find 120 sgRNAs with CRISPR-Local

Find 320 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCACTCCATTGTTCTTGTTT+TGG	0.149888	1_4:+15329281	None:intergenic
TAAGTTCTATTGCCCGTTTA+AGG	0.213987	1_4:-15328603	Msa0152530:CDS
ACAATTCACTTGAAATAGTT+TGG	0.230706	1_4:-15329676	Msa0152530:five_prime_UTR
ACAGTTTCAAGAAACCTTAT+TGG	0.239473	1_4:-15329101	Msa0152530:CDS
TATGAAAAGGTTAATATAAA+TGG	0.241379	1_4:+15329529	None:intergenic
CACCTATCAATCAACTCTTT+TGG	0.279931	1_4:+15328665	None:intergenic
ATTATTTATTGTATTGGAAT+TGG	0.281076	1_4:-15329715	Msa0152530:five_prime_UTR
GCTATGCTTGCGGTGCAAAA+TGG	0.311093	1_4:-15327860	Msa0152530:CDS
CAATTCACTTGAAATAGTTT+GGG	0.315892	1_4:-15329675	Msa0152530:five_prime_UTR
TGTCATGTCACTTGGCATCC+TGG	0.318346	1_4:-15328488	Msa0152530:CDS
ATGAAGGAAGAGAAGATGTT+AGG	0.319698	1_4:-15328553	Msa0152530:CDS
TTAGTCATTATTTATTGTAT+TGG	0.328264	1_4:-15329721	Msa0152530:five_prime_UTR
GGTGGAGGCTATATCTTTAC+AGG	0.331943	1_4:+15327524	None:intergenic
GTCATGTCACTTGGCATCCT+GGG	0.343483	1_4:-15328487	Msa0152530:CDS
TGTTAGAGCTTGAATCTTGC+AGG	0.348834	1_4:-15328697	Msa0152530:CDS
CTTGAAATAGTTTGGGGAAG+TGG	0.350279	1_4:-15329668	Msa0152530:five_prime_UTR
TGAAGGAAGAGAAGATGTTA+GGG	0.359913	1_4:-15328552	Msa0152530:CDS
ATTTCAGAACAATCCTTAAA+CGG	0.364133	1_4:+15328590	None:intergenic
AGGAAAAGGATGAAGGGTTT+TGG	0.374297	1_4:+15329059	None:intergenic
CACCCTTTCTTAGAGACTCT+TGG	0.377414	1_4:-15327505	Msa0152530:three_prime_UTR
AGATTTGTGCGGAAACAAAA+AGG	0.391913	1_4:-15328850	Msa0152530:CDS
AGTGATGACAGAGGCCAATA+AGG	0.404886	1_4:+15329087	None:intergenic
TCATCGGAGACAGGGACGTT+AGG	0.409844	1_4:+15329142	None:intergenic
ACACATTTGTTATGTTGTCT+TGG	0.442504	1_4:+15328747	None:intergenic
TTTCAGAACAATCCTTAAAC+GGG	0.442510	1_4:+15328591	None:intergenic
TTGTGGCTGGTATGTCTCTC+AGG	0.444742	1_4:+15327453	None:intergenic
ATTCAACTGTAGACTTCAAA+AGG	0.452734	1_4:+15329370	None:intergenic
CTAATAAGAAAAGGTGGAAA+AGG	0.462208	1_4:-15328391	Msa0152530:CDS
GCAATGGTGGTGACTGCATA+AGG	0.463424	1_4:+15328957	None:intergenic
ATCTGTCTCTTTAAACAGTA+AGG	0.464117	1_4:+15327736	None:intergenic
TTCCAAAAGAGTTGATTGAT+AGG	0.467343	1_4:-15328667	Msa0152530:CDS
TCAACATAGAACCTGCATCT+TGG	0.469195	1_4:+15328365	None:intergenic
AAAGGACTGAGGAGATGTTA+AGG	0.472620	1_4:-15328832	Msa0152530:CDS
GTCACTTGGCATCCTGGGGT+TGG	0.476032	1_4:-15328482	Msa0152530:CDS
GAGGATGGTTGTTGCGCCAT+TGG	0.477772	1_4:+15329232	None:intergenic
AACGAACGAGTTTATTAAAG+TGG	0.477849	1_4:+15329318	None:intergenic
ATCAAGACGTGCATGATTCA+CGG	0.481390	1_4:+15327608	None:intergenic
ATAACAAATGTGTCTTGTCC+TGG	0.488527	1_4:-15328737	Msa0152530:CDS
GTTAGGGTCAATTTCATTGT+CGG	0.489721	1_4:+15329159	None:intergenic
GGGTGAGTGTGAAAGGTTAG+TGG	0.489847	1_4:+15329340	None:intergenic
AAACCAAGAGTCTCTAAGAA+AGG	0.490014	1_4:+15327502	None:intergenic
CATCGGAGACAGGGACGTTA+GGG	0.494082	1_4:+15329143	None:intergenic
TAAAGTGGGGTGAGTGTGAA+AGG	0.494266	1_4:+15329333	None:intergenic
TTGTCCTGGTTTGAACTGCA+AGG	0.494597	1_4:-15328723	Msa0152530:CDS
GCGTACTTGTCATCGGAGAC+AGG	0.495039	1_4:+15329133	None:intergenic
ACAAAGAAGCATATATGAAA+AGG	0.495634	1_4:+15329516	None:intergenic
GGGATGAAATATATTTATTG+TGG	0.498213	1_4:-15327791	Msa0152530:three_prime_UTR
GGAAAAGGTGTCCAAGATGC+AGG	0.500394	1_4:-15328376	Msa0152530:CDS
GCATCCTGGGGTTGGTTGTG+AGG	0.501263	1_4:-15328474	Msa0152530:CDS
TCTTCTTCCTCAAAATCTCT+CGG	0.506168	1_4:-15329592	Msa0152530:five_prime_UTR
TCATCGAAGGCTATTGATGT+TGG	0.512485	1_4:-15328920	Msa0152530:CDS
AAATTAGATAGATGCAAGAA+GGG	0.513950	1_4:-15327811	Msa0152530:exon
ATAGCAAGTACACTTTCAAA+AGG	0.515766	1_4:+15327645	None:intergenic
TGAATGTGGATGAAAGAGGA+AGG	0.518591	1_4:-15328439	Msa0152530:CDS
GTGCAAGCTGTCATGTCACT+TGG	0.521854	1_4:-15328496	Msa0152530:CDS
GAAATTAGATAGATGCAAGA+AGG	0.523692	1_4:-15327812	Msa0152530:exon
GCCAAGAAGGAAAAGGATGA+AGG	0.524777	1_4:+15329052	None:intergenic
CACATTCAGAACAAAATGAA+TGG	0.525752	1_4:+15328795	None:intergenic
CATCATTTGCGAGATTTGTG+CGG	0.525830	1_4:-15328861	Msa0152530:CDS
AGATCTCCTTGTGAGGGAGC+TGG	0.527981	1_4:-15328414	Msa0152530:CDS
TTAAACGGGCAATAGAACTT+AGG	0.535232	1_4:+15328605	None:intergenic
AACCAAGAGTCTCTAAGAAA+GGG	0.546527	1_4:+15327503	None:intergenic
GATGTTTGCATATTACGTGC+AGG	0.550867	1_4:-15328331	Msa0152530:intron
AGACTTCAAAAGGAGTAAAG+AGG	0.551229	1_4:+15329380	None:intergenic
GGTGACTGCATAAGGGTTTG+AGG	0.562459	1_4:+15328965	None:intergenic
ACGAACGAGTTTATTAAAGT+GGG	0.563896	1_4:+15329319	None:intergenic
CGTCTACGGTGTCAAGGACA+AGG	0.566082	1_4:+15329203	None:intergenic
TTCTGAAATGTTGCTTGATG+AGG	0.567131	1_4:-15328576	Msa0152530:CDS
TGCTTCAGCGTACTTGTCAT+CGG	0.567735	1_4:+15329126	None:intergenic
CAAAACAATCTATGACAAGA+AGG	0.568712	1_4:+15328518	None:intergenic
TATTAGCCAGCTCCCTCACA+AGG	0.570249	1_4:+15328408	None:intergenic
TAAGGAACTTCCCCTATCCA+TGG	0.571888	1_4:+15327754	None:intergenic
TGTCCTGGTTTGAACTGCAA+GGG	0.576754	1_4:-15328722	Msa0152530:CDS
GGAAGAAGAAAGTGACAGAG+AGG	0.577157	1_4:+15329606	None:intergenic
GGGAGCTGGCTAATAAGAAA+AGG	0.577777	1_4:-15328400	Msa0152530:CDS
GGTGTCAAGGACAAGGAATG+AGG	0.580931	1_4:+15329210	None:intergenic
AATTTCATTGTCGGAGACAA+CGG	0.581243	1_4:+15329168	None:intergenic
CAAAAGAGTTGATTGATAGG+TGG	0.583180	1_4:-15328664	Msa0152530:CDS
TCGGTCATCACTATCATCAT+CGG	0.586764	1_4:-15329573	Msa0152530:five_prime_UTR
TCATGTCACTTGGCATCCTG+GGG	0.587704	1_4:-15328486	Msa0152530:CDS
AAAAGTCGTCTACGGTGTCA+AGG	0.592520	1_4:+15329197	None:intergenic
GATGCTACACACGGTAATGA+TGG	0.595205	1_4:+15329022	None:intergenic
CTGCGTAGCATCTTCATCGA+AGG	0.598053	1_4:-15328933	Msa0152530:CDS
ACACCCTTGCAGTTCAAACC+AGG	0.598898	1_4:+15328719	None:intergenic
GCAAAATGGACATCTTCTCA+TGG	0.606786	1_4:-15327846	Msa0152530:CDS
CAAATCTCGCAAATGATGAT+CGG	0.607678	1_4:+15328866	None:intergenic
CAAGAGTCTCTAAGAAAGGG+TGG	0.613022	1_4:+15327506	None:intergenic
TGATGGTGGTGGTGCCAAGA+AGG	0.616247	1_4:+15329039	None:intergenic
GGAGTTTCAAACAATGAATG+TGG	0.616690	1_4:-15328453	Msa0152530:CDS
ACAATGAATGTGGATGAAAG+AGG	0.616843	1_4:-15328443	Msa0152530:CDS
CCAAGAAGGAAAAGGATGAA+GGG	0.617075	1_4:+15329053	None:intergenic
CAATGGTGGTGACTGCATAA+GGG	0.617916	1_4:+15328958	None:intergenic
GAATGTGGATGAAAGAGGAA+GGG	0.623790	1_4:-15328438	Msa0152530:CDS
GAAGGGAAGATCTCCTTGTG+AGG	0.624223	1_4:-15328421	Msa0152530:CDS
TTTGATATATCCATGGATAG+GGG	0.625407	1_4:-15327764	Msa0152530:three_prime_UTR
TGGTGGTGCCAAGAAGGAAA+AGG	0.625615	1_4:+15329045	None:intergenic
AAGGGAAGATCTCCTTGTGA+GGG	0.626069	1_4:-15328420	Msa0152530:CDS
AACTCCTCACAACCAACCCC+AGG	0.626419	1_4:+15328470	None:intergenic
AGCTGGCTAATAAGAAAAGG+TGG	0.627214	1_4:-15328397	Msa0152530:CDS
AAAACAATCTATGACAAGAA+GGG	0.629017	1_4:+15328519	None:intergenic
GGAGAAGTAAAAGTCGTCTA+CGG	0.631739	1_4:+15329189	None:intergenic
AATGACTAAAATCATAAACA+TGG	0.634198	1_4:+15329735	None:intergenic
TTTCCCCAAAACAAGAACAA+TGG	0.635586	1_4:-15329286	Msa0152530:five_prime_UTR
AGGACAAGGAATGAGGAGGA+TGG	0.637593	1_4:+15329217	None:intergenic
ACTATTTCAAGTGAATTGTG+AGG	0.638641	1_4:+15329680	None:intergenic
CGTACTTGTCATCGGAGACA+GGG	0.638650	1_4:+15329134	None:intergenic
AATTCACTTGAAATAGTTTG+GGG	0.644524	1_4:-15329674	Msa0152530:five_prime_UTR
GAGTCTCTAAGAAAGGGTGG+AGG	0.645832	1_4:+15327509	None:intergenic
TGAATGTGTTGTCAAACAAG+TGG	0.654131	1_4:-15328780	Msa0152530:CDS
GATGCTACGCAGTGCAATGG+TGG	0.655147	1_4:+15328944	None:intergenic
CGAACGAGTTTATTAAAGTG+GGG	0.657987	1_4:+15329320	None:intergenic
GTCAAGGACAAGGAATGAGG+AGG	0.658566	1_4:+15329213	None:intergenic
GAAGATGCTACGCAGTGCAA+TGG	0.668125	1_4:+15328941	None:intergenic
ATGTTGCTTGATGAGGATGA+AGG	0.680073	1_4:-15328569	Msa0152530:CDS
TTTGATGAAGATGCTACACA+CGG	0.680690	1_4:+15329013	None:intergenic
ACACACGGTAATGATGGTGG+TGG	0.696914	1_4:+15329028	None:intergenic
GTGGAATGATGCATTGTGTG+AGG	0.727329	1_4:-15328645	Msa0152530:CDS
GCTACACACGGTAATGATGG+TGG	0.734605	1_4:+15329025	None:intergenic
TTGGTGAGAAGTGATGACAG+AGG	0.745687	1_4:+15329078	None:intergenic
AAATGGACATCTTCTCATGG+TGG	0.767144	1_4:-15327843	Msa0152530:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAGTCATTATTTATTGTAT+TGG	-	chr1_4:15327471-15327490	Msa0152530:three_prime_UTR	15.0%
!!	ATTATTTATTGTATTGGAAT+TGG	-	chr1_4:15327477-15327496	Msa0152530:three_prime_UTR	15.0%
!!	TATGAAAAGGTTAATATAAA+TGG	+	chr1_4:15327666-15327685	None:intergenic	15.0%
!!	TGAAAATATATAAAAAGTGA+AGG	+	chr1_4:15327768-15327787	None:intergenic	15.0%
!!!	TTTTTTAAATTGTGTGTTTT+TGG	-	chr1_4:15328976-15328995	Msa0152530:CDS	15.0%
!!	AATTAACAAAGTAAAACTAA+AGG	+	chr1_4:15329032-15329051	None:intergenic	15.0%
!!	TACAACAATTTCTTAATTAA+GGG	+	chr1_4:15329248-15329267	None:intergenic	15.0%
!!	TTACAACAATTTCTTAATTA+AGG	+	chr1_4:15329249-15329268	None:intergenic	15.0%
!!	TTAGTCATTATTTATTGTAT+TGG	-	chr1_4:15327471-15327490	Msa0152530:three_prime_UTR	15.0%
!!	ATTATTTATTGTATTGGAAT+TGG	-	chr1_4:15327477-15327496	Msa0152530:three_prime_UTR	15.0%
!!	TATGAAAAGGTTAATATAAA+TGG	+	chr1_4:15327666-15327685	None:intergenic	15.0%
!!	TGAAAATATATAAAAAGTGA+AGG	+	chr1_4:15327768-15327787	None:intergenic	15.0%
!!!	TTTTTTAAATTGTGTGTTTT+TGG	-	chr1_4:15328976-15328995	Msa0152530:CDS	15.0%
!!	AATTAACAAAGTAAAACTAA+AGG	+	chr1_4:15329032-15329051	None:intergenic	15.0%
!!	TACAACAATTTCTTAATTAA+GGG	+	chr1_4:15329248-15329267	None:intergenic	15.0%
!!	TTACAACAATTTCTTAATTA+AGG	+	chr1_4:15329249-15329268	None:intergenic	15.0%
!!!	ATTCAACTCTTTTCTATTTT+CGG	-	chr1_4:15328904-15328923	Msa0152530:CDS	20.0%
!!!	TGTTTAACGTTTTGATTAAA+AGG	-	chr1_4:15329174-15329193	Msa0152530:CDS	20.0%
!!!	ATTCAACTCTTTTCTATTTT+CGG	-	chr1_4:15328904-15328923	Msa0152530:CDS	20.0%
!!!	TGTTTAACGTTTTGATTAAA+AGG	-	chr1_4:15329174-15329193	Msa0152530:CDS	20.0%
!	ACAATTCACTTGAAATAGTT+TGG	-	chr1_4:15327516-15327535	Msa0152530:three_prime_UTR	25.0%
!	CAATTCACTTGAAATAGTTT+GGG	-	chr1_4:15327517-15327536	Msa0152530:three_prime_UTR	25.0%
!	AATTCACTTGAAATAGTTTG+GGG	-	chr1_4:15327518-15327537	Msa0152530:three_prime_UTR	25.0%
!!	ACAAAGAAGCATATATGAAA+AGG	+	chr1_4:15327679-15327698	None:intergenic	25.0%
!!	TAAGTTTTTGTGTGTTTATG+TGG	+	chr1_4:15327938-15327957	None:intergenic	25.0%
!	ATTTCAGAACAATCCTTAAA+CGG	+	chr1_4:15328605-15328624	None:intergenic	25.0%
!	AAAACAATCTATGACAAGAA+GGG	+	chr1_4:15328676-15328695	None:intergenic	25.0%
!!!	TATCATTTTTTGTGTCACTT+TGG	-	chr1_4:15329005-15329024	Msa0152530:CDS	25.0%
!	AAATGAACGATATTGATCTT+TGG	+	chr1_4:15329140-15329159	None:intergenic	25.0%
!	TTAATCAAAACGTTAAACAG+TGG	+	chr1_4:15329173-15329192	None:intergenic	25.0%
!!!	TAACGTTTTGATTAAAAGGA+AGG	-	chr1_4:15329178-15329197	Msa0152530:CDS	25.0%
!!	TACCATGATTTAACTTATGA+TGG	+	chr1_4:15329205-15329224	None:intergenic	25.0%
!	AAATTAGATAGATGCAAGAA+GGG	-	chr1_4:15329381-15329400	Msa0152530:five_prime_UTR	25.0%
!	GGGATGAAATATATTTATTG+TGG	-	chr1_4:15329401-15329420	Msa0152530:five_prime_UTR	25.0%
!!	TTTTGATATATCCATGGATA+GGG	-	chr1_4:15329427-15329446	Msa0152530:five_prime_UTR	25.0%
!	ACAATTCACTTGAAATAGTT+TGG	-	chr1_4:15327516-15327535	Msa0152530:three_prime_UTR	25.0%
!	CAATTCACTTGAAATAGTTT+GGG	-	chr1_4:15327517-15327536	Msa0152530:three_prime_UTR	25.0%
!	AATTCACTTGAAATAGTTTG+GGG	-	chr1_4:15327518-15327537	Msa0152530:three_prime_UTR	25.0%
!!	ACAAAGAAGCATATATGAAA+AGG	+	chr1_4:15327679-15327698	None:intergenic	25.0%
!!	TAAGTTTTTGTGTGTTTATG+TGG	+	chr1_4:15327938-15327957	None:intergenic	25.0%
!	ATTTCAGAACAATCCTTAAA+CGG	+	chr1_4:15328605-15328624	None:intergenic	25.0%
!	AAAACAATCTATGACAAGAA+GGG	+	chr1_4:15328676-15328695	None:intergenic	25.0%
!!!	TATCATTTTTTGTGTCACTT+TGG	-	chr1_4:15329005-15329024	Msa0152530:CDS	25.0%
!	AAATGAACGATATTGATCTT+TGG	+	chr1_4:15329140-15329159	None:intergenic	25.0%
!	TTAATCAAAACGTTAAACAG+TGG	+	chr1_4:15329173-15329192	None:intergenic	25.0%
!!!	TAACGTTTTGATTAAAAGGA+AGG	-	chr1_4:15329178-15329197	Msa0152530:CDS	25.0%
!!	TACCATGATTTAACTTATGA+TGG	+	chr1_4:15329205-15329224	None:intergenic	25.0%
!	AAATTAGATAGATGCAAGAA+GGG	-	chr1_4:15329381-15329400	Msa0152530:five_prime_UTR	25.0%
!	GGGATGAAATATATTTATTG+TGG	-	chr1_4:15329401-15329420	Msa0152530:five_prime_UTR	25.0%
!!	TTTTGATATATCCATGGATA+GGG	-	chr1_4:15329427-15329446	Msa0152530:five_prime_UTR	25.0%
!	ACTATTTCAAGTGAATTGTG+AGG	+	chr1_4:15327515-15327534	None:intergenic	30.0%
	ATTCAACTGTAGACTTCAAA+AGG	+	chr1_4:15327825-15327844	None:intergenic	30.0%
	ACGAACGAGTTTATTAAAGT+GGG	+	chr1_4:15327876-15327895	None:intergenic	30.0%
	AACGAACGAGTTTATTAAAG+TGG	+	chr1_4:15327877-15327896	None:intergenic	30.0%
	ACAGTTTCAAGAAACCTTAT+TGG	-	chr1_4:15328091-15328110	Msa0152530:intron	30.0%
	CACATTCAGAACAAAATGAA+TGG	+	chr1_4:15328400-15328419	None:intergenic	30.0%
!!	ACACATTTGTTATGTTGTCT+TGG	+	chr1_4:15328448-15328467	None:intergenic	30.0%
!	TTCCAAAAGAGTTGATTGAT+AGG	-	chr1_4:15328525-15328544	Msa0152530:CDS	30.0%
	TTTCAGAACAATCCTTAAAC+GGG	+	chr1_4:15328604-15328623	None:intergenic	30.0%
	CAAAACAATCTATGACAAGA+AGG	+	chr1_4:15328677-15328696	None:intergenic	30.0%
	CTAATAAGAAAAGGTGGAAA+AGG	-	chr1_4:15328801-15328820	Msa0152530:CDS	30.0%
	CAGGTTCTATGTTGAAAAAA+GGG	-	chr1_4:15328835-15328854	Msa0152530:CDS	30.0%
	TTCTATGTTGAAAAAAGGGA+TGG	-	chr1_4:15328839-15328858	Msa0152530:CDS	30.0%
	TAAGTATGAGATGATTGTCT+TGG	-	chr1_4:15329058-15329077	Msa0152530:CDS	30.0%
	GACCATCATAAGTTAAATCA+TGG	-	chr1_4:15329200-15329219	Msa0152530:CDS	30.0%
	TTCTTAATTAAGGGTCACTT+TGG	+	chr1_4:15329239-15329258	None:intergenic	30.0%
	AATCAGTGAACAACTGAATA+AGG	-	chr1_4:15329275-15329294	Msa0152530:five_prime_UTR	30.0%
	ATCAGTGAACAACTGAATAA+GGG	-	chr1_4:15329276-15329295	Msa0152530:five_prime_UTR	30.0%
	GAAATTAGATAGATGCAAGA+AGG	-	chr1_4:15329380-15329399	Msa0152530:five_prime_UTR	30.0%
!!!	TGGTTGTTTTGATATATCCA+TGG	-	chr1_4:15329421-15329440	Msa0152530:five_prime_UTR	30.0%
!!	GTTTTGATATATCCATGGAT+AGG	-	chr1_4:15329426-15329445	Msa0152530:five_prime_UTR	30.0%
	TTTGATATATCCATGGATAG+GGG	-	chr1_4:15329428-15329447	Msa0152530:five_prime_UTR	30.0%
	ATCTGTCTCTTTAAACAGTA+AGG	+	chr1_4:15329459-15329478	None:intergenic	30.0%
	ATAGCAAGTACACTTTCAAA+AGG	+	chr1_4:15329550-15329569	None:intergenic	30.0%
!	ACTATTTCAAGTGAATTGTG+AGG	+	chr1_4:15327515-15327534	None:intergenic	30.0%
	ATTCAACTGTAGACTTCAAA+AGG	+	chr1_4:15327825-15327844	None:intergenic	30.0%
	ACGAACGAGTTTATTAAAGT+GGG	+	chr1_4:15327876-15327895	None:intergenic	30.0%
	AACGAACGAGTTTATTAAAG+TGG	+	chr1_4:15327877-15327896	None:intergenic	30.0%
	ACAGTTTCAAGAAACCTTAT+TGG	-	chr1_4:15328091-15328110	Msa0152530:intron	30.0%
	CACATTCAGAACAAAATGAA+TGG	+	chr1_4:15328400-15328419	None:intergenic	30.0%
!!	ACACATTTGTTATGTTGTCT+TGG	+	chr1_4:15328448-15328467	None:intergenic	30.0%
!	TTCCAAAAGAGTTGATTGAT+AGG	-	chr1_4:15328525-15328544	Msa0152530:CDS	30.0%
	TTTCAGAACAATCCTTAAAC+GGG	+	chr1_4:15328604-15328623	None:intergenic	30.0%
	CAAAACAATCTATGACAAGA+AGG	+	chr1_4:15328677-15328696	None:intergenic	30.0%
	CTAATAAGAAAAGGTGGAAA+AGG	-	chr1_4:15328801-15328820	Msa0152530:CDS	30.0%
	CAGGTTCTATGTTGAAAAAA+GGG	-	chr1_4:15328835-15328854	Msa0152530:CDS	30.0%
	TTCTATGTTGAAAAAAGGGA+TGG	-	chr1_4:15328839-15328858	Msa0152530:CDS	30.0%
	TAAGTATGAGATGATTGTCT+TGG	-	chr1_4:15329058-15329077	Msa0152530:CDS	30.0%
	GACCATCATAAGTTAAATCA+TGG	-	chr1_4:15329200-15329219	Msa0152530:CDS	30.0%
	TTCTTAATTAAGGGTCACTT+TGG	+	chr1_4:15329239-15329258	None:intergenic	30.0%
	AATCAGTGAACAACTGAATA+AGG	-	chr1_4:15329275-15329294	Msa0152530:five_prime_UTR	30.0%
	ATCAGTGAACAACTGAATAA+GGG	-	chr1_4:15329276-15329295	Msa0152530:five_prime_UTR	30.0%
	GAAATTAGATAGATGCAAGA+AGG	-	chr1_4:15329380-15329399	Msa0152530:five_prime_UTR	30.0%
!!!	TGGTTGTTTTGATATATCCA+TGG	-	chr1_4:15329421-15329440	Msa0152530:five_prime_UTR	30.0%
!!	GTTTTGATATATCCATGGAT+AGG	-	chr1_4:15329426-15329445	Msa0152530:five_prime_UTR	30.0%
	TTTGATATATCCATGGATAG+GGG	-	chr1_4:15329428-15329447	Msa0152530:five_prime_UTR	30.0%
	ATCTGTCTCTTTAAACAGTA+AGG	+	chr1_4:15329459-15329478	None:intergenic	30.0%
	ATAGCAAGTACACTTTCAAA+AGG	+	chr1_4:15329550-15329569	None:intergenic	30.0%
	TCTTCTTCCTCAAAATCTCT+CGG	-	chr1_4:15327600-15327619	Msa0152530:three_prime_UTR	35.0%
	AGACTTCAAAAGGAGTAAAG+AGG	+	chr1_4:15327815-15327834	None:intergenic	35.0%
	CGAACGAGTTTATTAAAGTG+GGG	+	chr1_4:15327875-15327894	None:intergenic	35.0%
!!!	TGTTCTTGTTTTGGGGAAAT+GGG	+	chr1_4:15327905-15327924	None:intergenic	35.0%
!!!	TTGTTCTTGTTTTGGGGAAA+TGG	+	chr1_4:15327906-15327925	None:intergenic	35.0%
	TTTCCCCAAAACAAGAACAA+TGG	-	chr1_4:15327906-15327925	Msa0152530:intron	35.0%
!!!	ACTCCATTGTTCTTGTTTTG+GGG	+	chr1_4:15327912-15327931	None:intergenic	35.0%
!!!	CACTCCATTGTTCTTGTTTT+GGG	+	chr1_4:15327913-15327932	None:intergenic	35.0%
!!	TCACTCCATTGTTCTTGTTT+TGG	+	chr1_4:15327914-15327933	None:intergenic	35.0%
	CACACAAAAACTTACACCAA+TGG	-	chr1_4:15327944-15327963	Msa0152530:intron	35.0%
	AATTTCATTGTCGGAGACAA+CGG	+	chr1_4:15328027-15328046	None:intergenic	35.0%
	GTTAGGGTCAATTTCATTGT+CGG	+	chr1_4:15328036-15328055	None:intergenic	35.0%
	TTTGATGAAGATGCTACACA+CGG	+	chr1_4:15328182-15328201	None:intergenic	35.0%
	CAAATCTCGCAAATGATGAT+CGG	+	chr1_4:15328329-15328348	None:intergenic	35.0%
	AGATTTGTGCGGAAACAAAA+AGG	-	chr1_4:15328342-15328361	Msa0152530:CDS	35.0%
	TGAATGTGTTGTCAAACAAG+TGG	-	chr1_4:15328412-15328431	Msa0152530:CDS	35.0%
	ATAACAAATGTGTCTTGTCC+TGG	-	chr1_4:15328455-15328474	Msa0152530:CDS	35.0%
!	CACCTATCAATCAACTCTTT+TGG	+	chr1_4:15328530-15328549	None:intergenic	35.0%
!	CAAAAGAGTTGATTGATAGG+TGG	-	chr1_4:15328528-15328547	Msa0152530:CDS	35.0%
	TTAAACGGGCAATAGAACTT+AGG	+	chr1_4:15328590-15328609	None:intergenic	35.0%
	TAAGTTCTATTGCCCGTTTA+AGG	-	chr1_4:15328589-15328608	Msa0152530:CDS	35.0%
!!	TTCTGAAATGTTGCTTGATG+AGG	-	chr1_4:15328616-15328635	Msa0152530:CDS	35.0%
	ATGAAGGAAGAGAAGATGTT+AGG	-	chr1_4:15328639-15328658	Msa0152530:CDS	35.0%
	TGAAGGAAGAGAAGATGTTA+GGG	-	chr1_4:15328640-15328659	Msa0152530:CDS	35.0%
	GGAGTTTCAAACAATGAATG+TGG	-	chr1_4:15328739-15328758	Msa0152530:CDS	35.0%
	ACAATGAATGTGGATGAAAG+AGG	-	chr1_4:15328749-15328768	Msa0152530:CDS	35.0%
	GCAGGTTCTATGTTGAAAAA+AGG	-	chr1_4:15328834-15328853	Msa0152530:CDS	35.0%
	TGATTGTCTTGGCATTCATT+TGG	-	chr1_4:15329069-15329088	Msa0152530:CDS	35.0%
!!	TTTGAGTTTTGCTATGCTTG+CGG	-	chr1_4:15329322-15329341	Msa0152530:five_prime_UTR	35.0%
!	AACCAAGAGTCTCTAAGAAA+GGG	+	chr1_4:15329692-15329711	None:intergenic	35.0%
!	AAACCAAGAGTCTCTAAGAA+AGG	+	chr1_4:15329693-15329712	None:intergenic	35.0%
	TCTTCTTCCTCAAAATCTCT+CGG	-	chr1_4:15327600-15327619	Msa0152530:three_prime_UTR	35.0%
	AGACTTCAAAAGGAGTAAAG+AGG	+	chr1_4:15327815-15327834	None:intergenic	35.0%
	CGAACGAGTTTATTAAAGTG+GGG	+	chr1_4:15327875-15327894	None:intergenic	35.0%
!!!	TGTTCTTGTTTTGGGGAAAT+GGG	+	chr1_4:15327905-15327924	None:intergenic	35.0%
!!!	TTGTTCTTGTTTTGGGGAAA+TGG	+	chr1_4:15327906-15327925	None:intergenic	35.0%
	TTTCCCCAAAACAAGAACAA+TGG	-	chr1_4:15327906-15327925	Msa0152530:intron	35.0%
!!!	ACTCCATTGTTCTTGTTTTG+GGG	+	chr1_4:15327912-15327931	None:intergenic	35.0%
!!!	CACTCCATTGTTCTTGTTTT+GGG	+	chr1_4:15327913-15327932	None:intergenic	35.0%
!!	TCACTCCATTGTTCTTGTTT+TGG	+	chr1_4:15327914-15327933	None:intergenic	35.0%
	CACACAAAAACTTACACCAA+TGG	-	chr1_4:15327944-15327963	Msa0152530:intron	35.0%
	AATTTCATTGTCGGAGACAA+CGG	+	chr1_4:15328027-15328046	None:intergenic	35.0%
	GTTAGGGTCAATTTCATTGT+CGG	+	chr1_4:15328036-15328055	None:intergenic	35.0%
	TTTGATGAAGATGCTACACA+CGG	+	chr1_4:15328182-15328201	None:intergenic	35.0%
	CAAATCTCGCAAATGATGAT+CGG	+	chr1_4:15328329-15328348	None:intergenic	35.0%
	AGATTTGTGCGGAAACAAAA+AGG	-	chr1_4:15328342-15328361	Msa0152530:CDS	35.0%
	TGAATGTGTTGTCAAACAAG+TGG	-	chr1_4:15328412-15328431	Msa0152530:CDS	35.0%
	ATAACAAATGTGTCTTGTCC+TGG	-	chr1_4:15328455-15328474	Msa0152530:CDS	35.0%
!	CACCTATCAATCAACTCTTT+TGG	+	chr1_4:15328530-15328549	None:intergenic	35.0%
!	CAAAAGAGTTGATTGATAGG+TGG	-	chr1_4:15328528-15328547	Msa0152530:CDS	35.0%
	TTAAACGGGCAATAGAACTT+AGG	+	chr1_4:15328590-15328609	None:intergenic	35.0%
	TAAGTTCTATTGCCCGTTTA+AGG	-	chr1_4:15328589-15328608	Msa0152530:CDS	35.0%
!!	TTCTGAAATGTTGCTTGATG+AGG	-	chr1_4:15328616-15328635	Msa0152530:CDS	35.0%
	ATGAAGGAAGAGAAGATGTT+AGG	-	chr1_4:15328639-15328658	Msa0152530:CDS	35.0%
	TGAAGGAAGAGAAGATGTTA+GGG	-	chr1_4:15328640-15328659	Msa0152530:CDS	35.0%
	GGAGTTTCAAACAATGAATG+TGG	-	chr1_4:15328739-15328758	Msa0152530:CDS	35.0%
	ACAATGAATGTGGATGAAAG+AGG	-	chr1_4:15328749-15328768	Msa0152530:CDS	35.0%
	GCAGGTTCTATGTTGAAAAA+AGG	-	chr1_4:15328834-15328853	Msa0152530:CDS	35.0%
	TGATTGTCTTGGCATTCATT+TGG	-	chr1_4:15329069-15329088	Msa0152530:CDS	35.0%
!!	TTTGAGTTTTGCTATGCTTG+CGG	-	chr1_4:15329322-15329341	Msa0152530:five_prime_UTR	35.0%
!	AACCAAGAGTCTCTAAGAAA+GGG	+	chr1_4:15329692-15329711	None:intergenic	35.0%
!	AAACCAAGAGTCTCTAAGAA+AGG	+	chr1_4:15329693-15329712	None:intergenic	35.0%
	CTTGAAATAGTTTGGGGAAG+TGG	-	chr1_4:15327524-15327543	Msa0152530:three_prime_UTR	40.0%
	TCGGTCATCACTATCATCAT+CGG	-	chr1_4:15327619-15327638	Msa0152530:three_prime_UTR	40.0%
!!!	TTCTTGTTTTGGGGAAATGG+GGG	+	chr1_4:15327903-15327922	None:intergenic	40.0%
!!!	GTTCTTGTTTTGGGGAAATG+GGG	+	chr1_4:15327904-15327923	None:intergenic	40.0%
	GGAGAAGTAAAAGTCGTCTA+CGG	+	chr1_4:15328006-15328025	None:intergenic	40.0%
!	AGGAAAAGGATGAAGGGTTT+TGG	+	chr1_4:15328136-15328155	None:intergenic	40.0%
	CCAAGAAGGAAAAGGATGAA+GGG	+	chr1_4:15328142-15328161	None:intergenic	40.0%
	TCATCGAAGGCTATTGATGT+TGG	-	chr1_4:15328272-15328291	Msa0152530:intron	40.0%
	CATCATTTGCGAGATTTGTG+CGG	-	chr1_4:15328331-15328350	Msa0152530:intron	40.0%
	AAAGGACTGAGGAGATGTTA+AGG	-	chr1_4:15328360-15328379	Msa0152530:CDS	40.0%
	TGTTAGAGCTTGAATCTTGC+AGG	-	chr1_4:15328495-15328514	Msa0152530:CDS	40.0%
!	ATGTTGCTTGATGAGGATGA+AGG	-	chr1_4:15328623-15328642	Msa0152530:CDS	40.0%
	TGAATGTGGATGAAAGAGGA+AGG	-	chr1_4:15328753-15328772	Msa0152530:CDS	40.0%
	GAATGTGGATGAAAGAGGAA+GGG	-	chr1_4:15328754-15328773	Msa0152530:CDS	40.0%
	AGCTGGCTAATAAGAAAAGG+TGG	-	chr1_4:15328795-15328814	Msa0152530:CDS	40.0%
	TCAACATAGAACCTGCATCT+TGG	+	chr1_4:15328830-15328849	None:intergenic	40.0%
	GATGTTTGCATATTACGTGC+AGG	-	chr1_4:15328861-15328880	Msa0152530:CDS	40.0%
	GAAACCAATCCAAATAGCTC+CGG	+	chr1_4:15329095-15329114	None:intergenic	40.0%
	TAAGGGTGATGTTTGTTTGC+AGG	-	chr1_4:15329293-15329312	Msa0152530:five_prime_UTR	40.0%
	GCAAAATGGACATCTTCTCA+TGG	-	chr1_4:15329346-15329365	Msa0152530:five_prime_UTR	40.0%
	AAATGGACATCTTCTCATGG+TGG	-	chr1_4:15329349-15329368	Msa0152530:five_prime_UTR	40.0%
	ATCAAGACGTGCATGATTCA+CGG	+	chr1_4:15329587-15329606	None:intergenic	40.0%
	CTTGAAATAGTTTGGGGAAG+TGG	-	chr1_4:15327524-15327543	Msa0152530:three_prime_UTR	40.0%
	TCGGTCATCACTATCATCAT+CGG	-	chr1_4:15327619-15327638	Msa0152530:three_prime_UTR	40.0%
!!!	TTCTTGTTTTGGGGAAATGG+GGG	+	chr1_4:15327903-15327922	None:intergenic	40.0%
!!!	GTTCTTGTTTTGGGGAAATG+GGG	+	chr1_4:15327904-15327923	None:intergenic	40.0%
	GGAGAAGTAAAAGTCGTCTA+CGG	+	chr1_4:15328006-15328025	None:intergenic	40.0%
!	AGGAAAAGGATGAAGGGTTT+TGG	+	chr1_4:15328136-15328155	None:intergenic	40.0%
	CCAAGAAGGAAAAGGATGAA+GGG	+	chr1_4:15328142-15328161	None:intergenic	40.0%
	TCATCGAAGGCTATTGATGT+TGG	-	chr1_4:15328272-15328291	Msa0152530:intron	40.0%
	CATCATTTGCGAGATTTGTG+CGG	-	chr1_4:15328331-15328350	Msa0152530:intron	40.0%
	AAAGGACTGAGGAGATGTTA+AGG	-	chr1_4:15328360-15328379	Msa0152530:CDS	40.0%
	TGTTAGAGCTTGAATCTTGC+AGG	-	chr1_4:15328495-15328514	Msa0152530:CDS	40.0%
!	ATGTTGCTTGATGAGGATGA+AGG	-	chr1_4:15328623-15328642	Msa0152530:CDS	40.0%
	TGAATGTGGATGAAAGAGGA+AGG	-	chr1_4:15328753-15328772	Msa0152530:CDS	40.0%
	GAATGTGGATGAAAGAGGAA+GGG	-	chr1_4:15328754-15328773	Msa0152530:CDS	40.0%
	AGCTGGCTAATAAGAAAAGG+TGG	-	chr1_4:15328795-15328814	Msa0152530:CDS	40.0%
	TCAACATAGAACCTGCATCT+TGG	+	chr1_4:15328830-15328849	None:intergenic	40.0%
	GATGTTTGCATATTACGTGC+AGG	-	chr1_4:15328861-15328880	Msa0152530:CDS	40.0%
	GAAACCAATCCAAATAGCTC+CGG	+	chr1_4:15329095-15329114	None:intergenic	40.0%
	TAAGGGTGATGTTTGTTTGC+AGG	-	chr1_4:15329293-15329312	Msa0152530:five_prime_UTR	40.0%
	GCAAAATGGACATCTTCTCA+TGG	-	chr1_4:15329346-15329365	Msa0152530:five_prime_UTR	40.0%
	AAATGGACATCTTCTCATGG+TGG	-	chr1_4:15329349-15329368	Msa0152530:five_prime_UTR	40.0%
	ATCAAGACGTGCATGATTCA+CGG	+	chr1_4:15329587-15329606	None:intergenic	40.0%
	GGAAGAAGAAAGTGACAGAG+AGG	+	chr1_4:15327589-15327608	None:intergenic	45.0%
!	GTGATGACCGAGAGATTTTG+AGG	+	chr1_4:15327610-15327629	None:intergenic	45.0%
	TAAAGTGGGGTGAGTGTGAA+AGG	+	chr1_4:15327862-15327881	None:intergenic	45.0%
!	AAAAGTCGTCTACGGTGTCA+AGG	+	chr1_4:15327998-15328017	None:intergenic	45.0%
	TGCTTCAGCGTACTTGTCAT+CGG	+	chr1_4:15328069-15328088	None:intergenic	45.0%
	AGTGATGACAGAGGCCAATA+AGG	+	chr1_4:15328108-15328127	None:intergenic	45.0%
	TTGGTGAGAAGTGATGACAG+AGG	+	chr1_4:15328117-15328136	None:intergenic	45.0%
!	CCCTTCATCCTTTTCCTTCT+TGG	-	chr1_4:15328139-15328158	Msa0152530:intron	45.0%
	GCCAAGAAGGAAAAGGATGA+AGG	+	chr1_4:15328143-15328162	None:intergenic	45.0%
	GATGCTACACACGGTAATGA+TGG	+	chr1_4:15328173-15328192	None:intergenic	45.0%
!	CAATGGTGGTGACTGCATAA+GGG	+	chr1_4:15328237-15328256	None:intergenic	45.0%
	TGCGGAAACAAAAAGGACTG+AGG	-	chr1_4:15328349-15328368	Msa0152530:CDS	45.0%
!	TTGTCCTGGTTTGAACTGCA+AGG	-	chr1_4:15328469-15328488	Msa0152530:CDS	45.0%
!	TGTCCTGGTTTGAACTGCAA+GGG	-	chr1_4:15328470-15328489	Msa0152530:CDS	45.0%
	GTGGAATGATGCATTGTGTG+AGG	-	chr1_4:15328547-15328566	Msa0152530:CDS	45.0%
	AAGGGAAGATCTCCTTGTGA+GGG	-	chr1_4:15328772-15328791	Msa0152530:CDS	45.0%
	GGGAGCTGGCTAATAAGAAA+AGG	-	chr1_4:15328792-15328811	Msa0152530:CDS	45.0%
	TGTCTTGGCATTCATTTGGC+CGG	-	chr1_4:15329073-15329092	Msa0152530:CDS	45.0%
	TTCATTTGGCCGGAGCTATT+TGG	-	chr1_4:15329083-15329102	Msa0152530:CDS	45.0%
	TAAGGAACTTCCCCTATCCA+TGG	+	chr1_4:15329441-15329460	None:intergenic	45.0%
	GGTGGAGGCTATATCTTTAC+AGG	+	chr1_4:15329671-15329690	None:intergenic	45.0%
!	CAAGAGTCTCTAAGAAAGGG+TGG	+	chr1_4:15329689-15329708	None:intergenic	45.0%
	CACCCTTTCTTAGAGACTCT+TGG	-	chr1_4:15329687-15329706	Msa0152530:five_prime_UTR	45.0%
	GGAAGAAGAAAGTGACAGAG+AGG	+	chr1_4:15327589-15327608	None:intergenic	45.0%
!	GTGATGACCGAGAGATTTTG+AGG	+	chr1_4:15327610-15327629	None:intergenic	45.0%
	TAAAGTGGGGTGAGTGTGAA+AGG	+	chr1_4:15327862-15327881	None:intergenic	45.0%
!	AAAAGTCGTCTACGGTGTCA+AGG	+	chr1_4:15327998-15328017	None:intergenic	45.0%
	TGCTTCAGCGTACTTGTCAT+CGG	+	chr1_4:15328069-15328088	None:intergenic	45.0%
	AGTGATGACAGAGGCCAATA+AGG	+	chr1_4:15328108-15328127	None:intergenic	45.0%
	TTGGTGAGAAGTGATGACAG+AGG	+	chr1_4:15328117-15328136	None:intergenic	45.0%
!	CCCTTCATCCTTTTCCTTCT+TGG	-	chr1_4:15328139-15328158	Msa0152530:intron	45.0%
	GCCAAGAAGGAAAAGGATGA+AGG	+	chr1_4:15328143-15328162	None:intergenic	45.0%
	GATGCTACACACGGTAATGA+TGG	+	chr1_4:15328173-15328192	None:intergenic	45.0%
!	CAATGGTGGTGACTGCATAA+GGG	+	chr1_4:15328237-15328256	None:intergenic	45.0%
	TGCGGAAACAAAAAGGACTG+AGG	-	chr1_4:15328349-15328368	Msa0152530:CDS	45.0%
!	TTGTCCTGGTTTGAACTGCA+AGG	-	chr1_4:15328469-15328488	Msa0152530:CDS	45.0%
!	TGTCCTGGTTTGAACTGCAA+GGG	-	chr1_4:15328470-15328489	Msa0152530:CDS	45.0%
	GTGGAATGATGCATTGTGTG+AGG	-	chr1_4:15328547-15328566	Msa0152530:CDS	45.0%
	AAGGGAAGATCTCCTTGTGA+GGG	-	chr1_4:15328772-15328791	Msa0152530:CDS	45.0%
	GGGAGCTGGCTAATAAGAAA+AGG	-	chr1_4:15328792-15328811	Msa0152530:CDS	45.0%
	TGTCTTGGCATTCATTTGGC+CGG	-	chr1_4:15329073-15329092	Msa0152530:CDS	45.0%
	TTCATTTGGCCGGAGCTATT+TGG	-	chr1_4:15329083-15329102	Msa0152530:CDS	45.0%
	TAAGGAACTTCCCCTATCCA+TGG	+	chr1_4:15329441-15329460	None:intergenic	45.0%
	GGTGGAGGCTATATCTTTAC+AGG	+	chr1_4:15329671-15329690	None:intergenic	45.0%
!	CAAGAGTCTCTAAGAAAGGG+TGG	+	chr1_4:15329689-15329708	None:intergenic	45.0%
	CACCCTTTCTTAGAGACTCT+TGG	-	chr1_4:15329687-15329706	Msa0152530:five_prime_UTR	45.0%
	GGGTGAGTGTGAAAGGTTAG+TGG	+	chr1_4:15327855-15327874	None:intergenic	50.0%
!!!	TTGTTTTGGGGAAATGGGGG+AGG	+	chr1_4:15327900-15327919	None:intergenic	50.0%
	AGGACAAGGAATGAGGAGGA+TGG	+	chr1_4:15327978-15327997	None:intergenic	50.0%
	GTCAAGGACAAGGAATGAGG+AGG	+	chr1_4:15327982-15328001	None:intergenic	50.0%
	GGTGTCAAGGACAAGGAATG+AGG	+	chr1_4:15327985-15328004	None:intergenic	50.0%
	CGTACTTGTCATCGGAGACA+GGG	+	chr1_4:15328061-15328080	None:intergenic	50.0%
!	TGGTGGTGCCAAGAAGGAAA+AGG	+	chr1_4:15328150-15328169	None:intergenic	50.0%
!!	ACACACGGTAATGATGGTGG+TGG	+	chr1_4:15328167-15328186	None:intergenic	50.0%
	GCTACACACGGTAATGATGG+TGG	+	chr1_4:15328170-15328189	None:intergenic	50.0%
	GGTGACTGCATAAGGGTTTG+AGG	+	chr1_4:15328230-15328249	None:intergenic	50.0%
!	GCAATGGTGGTGACTGCATA+AGG	+	chr1_4:15328238-15328257	None:intergenic	50.0%
	GAAGATGCTACGCAGTGCAA+TGG	+	chr1_4:15328254-15328273	None:intergenic	50.0%
	CTGCGTAGCATCTTCATCGA+AGG	-	chr1_4:15328259-15328278	Msa0152530:intron	50.0%
	ACACCCTTGCAGTTCAAACC+AGG	+	chr1_4:15328476-15328495	None:intergenic	50.0%
	GTGCAAGCTGTCATGTCACT+TGG	-	chr1_4:15328696-15328715	Msa0152530:CDS	50.0%
!	TGTCATGTCACTTGGCATCC+TGG	-	chr1_4:15328704-15328723	Msa0152530:CDS	50.0%
!	GTCATGTCACTTGGCATCCT+GGG	-	chr1_4:15328705-15328724	Msa0152530:CDS	50.0%
!	TCATGTCACTTGGCATCCTG+GGG	-	chr1_4:15328706-15328725	Msa0152530:CDS	50.0%
	GAAGGGAAGATCTCCTTGTG+AGG	-	chr1_4:15328771-15328790	Msa0152530:CDS	50.0%
	TATTAGCCAGCTCCCTCACA+AGG	+	chr1_4:15328787-15328806	None:intergenic	50.0%
!!	GGAAAAGGTGTCCAAGATGC+AGG	-	chr1_4:15328816-15328835	Msa0152530:CDS	50.0%
	TTGGCCGGAGCTATTTGGAT+TGG	-	chr1_4:15329088-15329107	Msa0152530:CDS	50.0%
	GCTATGCTTGCGGTGCAAAA+TGG	-	chr1_4:15329332-15329351	Msa0152530:five_prime_UTR	50.0%
!	GAGTCTCTAAGAAAGGGTGG+AGG	+	chr1_4:15329686-15329705	None:intergenic	50.0%
	GGGTGAGTGTGAAAGGTTAG+TGG	+	chr1_4:15327855-15327874	None:intergenic	50.0%
!!!	TTGTTTTGGGGAAATGGGGG+AGG	+	chr1_4:15327900-15327919	None:intergenic	50.0%
	AGGACAAGGAATGAGGAGGA+TGG	+	chr1_4:15327978-15327997	None:intergenic	50.0%
	GTCAAGGACAAGGAATGAGG+AGG	+	chr1_4:15327982-15328001	None:intergenic	50.0%
	GGTGTCAAGGACAAGGAATG+AGG	+	chr1_4:15327985-15328004	None:intergenic	50.0%
	CGTACTTGTCATCGGAGACA+GGG	+	chr1_4:15328061-15328080	None:intergenic	50.0%
!	TGGTGGTGCCAAGAAGGAAA+AGG	+	chr1_4:15328150-15328169	None:intergenic	50.0%
!!	ACACACGGTAATGATGGTGG+TGG	+	chr1_4:15328167-15328186	None:intergenic	50.0%
	GCTACACACGGTAATGATGG+TGG	+	chr1_4:15328170-15328189	None:intergenic	50.0%
	GGTGACTGCATAAGGGTTTG+AGG	+	chr1_4:15328230-15328249	None:intergenic	50.0%
!	GCAATGGTGGTGACTGCATA+AGG	+	chr1_4:15328238-15328257	None:intergenic	50.0%
	GAAGATGCTACGCAGTGCAA+TGG	+	chr1_4:15328254-15328273	None:intergenic	50.0%
	CTGCGTAGCATCTTCATCGA+AGG	-	chr1_4:15328259-15328278	Msa0152530:intron	50.0%
	ACACCCTTGCAGTTCAAACC+AGG	+	chr1_4:15328476-15328495	None:intergenic	50.0%
	GTGCAAGCTGTCATGTCACT+TGG	-	chr1_4:15328696-15328715	Msa0152530:CDS	50.0%
!	TGTCATGTCACTTGGCATCC+TGG	-	chr1_4:15328704-15328723	Msa0152530:CDS	50.0%
!	GTCATGTCACTTGGCATCCT+GGG	-	chr1_4:15328705-15328724	Msa0152530:CDS	50.0%
!	TCATGTCACTTGGCATCCTG+GGG	-	chr1_4:15328706-15328725	Msa0152530:CDS	50.0%
	GAAGGGAAGATCTCCTTGTG+AGG	-	chr1_4:15328771-15328790	Msa0152530:CDS	50.0%
	TATTAGCCAGCTCCCTCACA+AGG	+	chr1_4:15328787-15328806	None:intergenic	50.0%
!!	GGAAAAGGTGTCCAAGATGC+AGG	-	chr1_4:15328816-15328835	Msa0152530:CDS	50.0%
	TTGGCCGGAGCTATTTGGAT+TGG	-	chr1_4:15329088-15329107	Msa0152530:CDS	50.0%
	GCTATGCTTGCGGTGCAAAA+TGG	-	chr1_4:15329332-15329351	Msa0152530:five_prime_UTR	50.0%
!	GAGTCTCTAAGAAAGGGTGG+AGG	+	chr1_4:15329686-15329705	None:intergenic	50.0%
	GAGGATGGTTGTTGCGCCAT+TGG	+	chr1_4:15327963-15327982	None:intergenic	55.0%
!	CGTCTACGGTGTCAAGGACA+AGG	+	chr1_4:15327992-15328011	None:intergenic	55.0%
	CATCGGAGACAGGGACGTTA+GGG	+	chr1_4:15328052-15328071	None:intergenic	55.0%
	TCATCGGAGACAGGGACGTT+AGG	+	chr1_4:15328053-15328072	None:intergenic	55.0%
	GCGTACTTGTCATCGGAGAC+AGG	+	chr1_4:15328062-15328081	None:intergenic	55.0%
!!	TGATGGTGGTGGTGCCAAGA+AGG	+	chr1_4:15328156-15328175	None:intergenic	55.0%
	GATGCTACGCAGTGCAATGG+TGG	+	chr1_4:15328251-15328270	None:intergenic	55.0%
	AACTCCTCACAACCAACCCC+AGG	+	chr1_4:15328725-15328744	None:intergenic	55.0%
	AGATCTCCTTGTGAGGGAGC+TGG	-	chr1_4:15328778-15328797	Msa0152530:CDS	55.0%
	GAGGATGGTTGTTGCGCCAT+TGG	+	chr1_4:15327963-15327982	None:intergenic	55.0%
!	CGTCTACGGTGTCAAGGACA+AGG	+	chr1_4:15327992-15328011	None:intergenic	55.0%
	CATCGGAGACAGGGACGTTA+GGG	+	chr1_4:15328052-15328071	None:intergenic	55.0%
	TCATCGGAGACAGGGACGTT+AGG	+	chr1_4:15328053-15328072	None:intergenic	55.0%
	GCGTACTTGTCATCGGAGAC+AGG	+	chr1_4:15328062-15328081	None:intergenic	55.0%
!!	TGATGGTGGTGGTGCCAAGA+AGG	+	chr1_4:15328156-15328175	None:intergenic	55.0%
	GATGCTACGCAGTGCAATGG+TGG	+	chr1_4:15328251-15328270	None:intergenic	55.0%
	AACTCCTCACAACCAACCCC+AGG	+	chr1_4:15328725-15328744	None:intergenic	55.0%
	AGATCTCCTTGTGAGGGAGC+TGG	-	chr1_4:15328778-15328797	Msa0152530:CDS	55.0%
	GTCACTTGGCATCCTGGGGT+TGG	-	chr1_4:15328710-15328729	Msa0152530:CDS	60.0%
!!	GCATCCTGGGGTTGGTTGTG+AGG	-	chr1_4:15328718-15328737	Msa0152530:CDS	60.0%
	GTCACTTGGCATCCTGGGGT+TGG	-	chr1_4:15328710-15328729	Msa0152530:CDS	60.0%
!!	GCATCCTGGGGTTGGTTGTG+AGG	-	chr1_4:15328718-15328737	Msa0152530:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	15327459	15329755	15327459	ID=Msa0152530;Name=Msa0152530
chr1_4	mRNA	15327459	15329755	15327459	ID=Msa0152530-mRNA-1;Parent=Msa0152530;Name=Msa0152530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=504\|1\|1\|1\|1\|1\|2\|368\|330
chr1_4	exon	15327459	15327899	15327459	ID=Msa0152530-mRNA-1:exon:5994;Parent=Msa0152530-mRNA-1
chr1_4	exon	15328332	15329755	15328332	ID=Msa0152530-mRNA-1:exon:5993;Parent=Msa0152530-mRNA-1
chr1_4	five_prime_UTR	15329252	15329755	15329252	ID=Msa0152530-mRNA-1:five_prime_utr;Parent=Msa0152530-mRNA-1
chr1_4	CDS	15328332	15329251	15328332	ID=Msa0152530-mRNA-1:cds;Parent=Msa0152530-mRNA-1
chr1_4	CDS	15327827	15327899	15327827	ID=Msa0152530-mRNA-1:cds;Parent=Msa0152530-mRNA-1
chr1_4	three_prime_UTR	15327459	15327826	15327459	ID=Msa0152530-mRNA-1:three_prime_utr;Parent=Msa0152530-mRNA-1

Gene Sequence

>Msa0152530

ATGGCGCAACAACCATCCTCCTCATTCCTTGTCCTTGACACCGTAGACGACTTTTACTTCTCCGTTGTCTCCGACAATGAAATTGACCCTAACGTCCCTGTCTCCGATGACAAGTACGCTGAAGCATTACAGTTTCAAGAAACCTTATTGGCCTCTGTCATCACTTCTCACCAAAACCCTTCATCCTTTTCCTTCTTGGCACCACCACCATCATTACCGTGTGTAGCATCTTCATCAAAAACAGTCTCTGAAGAGATACAGTTTCCTCAAACCCTTATGCAGTCACCACCATTGCACTGCGTAGCATCTTCATCGAAGGCTATTGATGTTGGCATTGTCTTCAAAGCTGATGAACAGTCTTCTCCGATCATCATTTGCGAGATTTGTGCGGAAACAAAAAGGACTGAGGAGATGTTAAGGAATCAAATATGTTACCATTCATTTTGTTCTGAATGTGTTGTCAAACAAGTGGCTACAAAAATCCAAGACAACATAACAAATGTGTCTTGTCCTGGTTTGAACTGCAAGGGTGTGTTAGAGCTTGAATCTTGCAGGTCATTGCTTCCAAAAGAGTTGATTGATAGGTGGAATGATGCATTGTGTGAGGCTTTTATTCTTGCTGTTCCTAAGTTCTATTGCCCGTTTAAGGATTGTTCTGAAATGTTGCTTGATGAGGATGAAGGAAGAGAAGATGTTAGGGAATCTGAGTGCCCTTCTTGTCATAGATTGTTTTGTGCAAGCTGTCATGTCACTTGGCATCCTGGGGTTGGTTGTGAGGAGTTTCAAACAATGAATGTGGATGAAAGAGGAAGGGAAGATCTCCTTGTGAGGGAGCTGGCTAATAAGAAAAGGTGGAAAAGGTGTCCAAGATGCAGGTTCTATGTTGAAAAAAGGGATGGATGTTTGCATATTACGTGCAGGTGCAAATTTGAGTTTTGCTATGCTTGCGGTGCAAAATGGACATCTTCTCATGGTGGTTGTCAAAGAAATTAG

Protein sequence

>Msa0152530

MAQQPSSSFLVLDTVDDFYFSVVSDNEIDPNVPVSDDKYAEALQFQETLLASVITSHQNPSSFSFLAPPPSLPCVASSSKTVSEEIQFPQTLMQSPPLHCVASSSKAIDVGIVFKADEQSSPIIICEICAETKRTEEMLRNQICYHSFCSECVVKQVATKIQDNITNVSCPGLNCKGVLELESCRSLLPKELIDRWNDALCEAFILAVPKFYCPFKDCSEMLLDEDEGREDVRESECPSCHRLFCASCHVTWHPGVGCEEFQTMNVDERGREDLLVRELANKKRWKRCPRCRFYVEKRDGCLHITCRCKFEFCYACGAKWTSSHGGCQRN*