AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0165650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165650 MtrunA17_Chr1g0191411 97.270 293 6 2 1 292 1 292 0.0 586
Msa0165650 MtrunA17_Chr7g0273211 70.877 285 80 1 2 286 34 315 3.16e-156 438
Msa0165650 MtrunA17_Chr5g0397331 52.060 267 124 3 5 269 82 346 1.09e-93 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165650 AT3G05170.1 72.222 270 75 0 3 272 7 276 3.20e-150 423
Msa0165650 AT1G08940.1 68.953 277 80 3 3 275 7 281 4.35e-142 401

Find 106 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGTTTCACCTTCATTT+TGG 0.169991 1_4:-46352146 None:intergenic
TTTCGGAAACTGCGAGTTTC+GGG 0.182047 1_4:+46351912 Msa0165650:CDS
ACTAATAAGTAACTAATTAA+AGG 0.229674 1_4:+46352183 Msa0165650:exon
CGCATTAGGATTATCCTCTT+TGG 0.271933 1_4:-46351246 None:intergenic
GACGCCGATCACGCGCTTCT+TGG 0.272814 1_4:-46351493 None:intergenic
CTAATTAAAGGGGAATTTAT+AGG 0.274139 1_4:+46352195 Msa0165650:three_prime_UTR
TTTGAGCATCTAAACAATTT+CGG 0.293790 1_4:+46351895 Msa0165650:CDS
TAGAATACAGTTTGGATGTA+TGG 0.300442 1_4:-46351075 None:intergenic
AATCTCCGCCGTCTCGTTTC+CGG 0.313382 1_4:+46351378 Msa0165650:CDS
CTTGAAATTAGAATTCAAAA+TGG 0.319343 1_4:+46351215 Msa0165650:exon
ATTTCGGAAACTGCGAGTTT+CGG 0.331893 1_4:+46351911 Msa0165650:CDS
CGCCGTCTCGTTTCCGGCCA+AGG 0.340582 1_4:+46351384 Msa0165650:CDS
GAGATTGGCGCCGGCGTGGC+GGG 0.342893 1_4:-46351361 None:intergenic
AAGAAACTCGTGAACGGTTT+GGG 0.344466 1_4:+46351586 Msa0165650:CDS
GTGACTCGCCGTGACGCATT+AGG 0.355053 1_4:-46351260 None:intergenic
AGAATCGCGCGTACGTGAAC+AGG 0.355351 1_4:+46351521 Msa0165650:CDS
GATAACCTTTGATTGATTGT+AGG 0.356988 1_4:+46352234 Msa0165650:three_prime_UTR
GCCAAGGCTGCTCCCCTGAT+TGG 0.360410 1_4:+46351400 Msa0165650:CDS
CTAATAAGTAACTAATTAAA+GGG 0.365006 1_4:+46352184 Msa0165650:exon
TGTGGACCGGGTGCGGGCGT+AGG 0.374827 1_4:-46351445 None:intergenic
GCCAATCAGGGGAGCAGCCT+TGG 0.377499 1_4:-46351401 None:intergenic
AAGGTTATCTTATCATCAAT+TGG 0.379933 1_4:-46352220 None:intergenic
TCGGCGGCGGATTCACCTTC+AGG 0.382270 1_4:-46351627 None:intergenic
CAATGTCCTTGGTATCTTGA+TGG 0.384985 1_4:+46352075 Msa0165650:CDS
AAAGAAACTCGTGAACGGTT+TGG 0.387033 1_4:+46351585 Msa0165650:CDS
ACCAGATTTAAATCGTTAGT+TGG 0.398319 1_4:-46351805 None:intergenic
ACTGTTTGTAGAATACAGTT+TGG 0.406791 1_4:-46351083 None:intergenic
CACGGAGTGTGGACCGGGTG+CGG 0.410340 1_4:-46351452 None:intergenic
AATCTAATTTCCAAAATTTG+TGG 0.411855 1_4:-46352368 None:intergenic
TATGTAATAATCATAATTTA+CGG 0.419568 1_4:+46352298 Msa0165650:three_prime_UTR
CGGAAGAAGAGATGCTTGAA+TGG 0.421203 1_4:+46351983 Msa0165650:CDS
CTAAACGATCCGATTGAGAT+TGG 0.432151 1_4:-46352413 None:intergenic
GTGGACCGGGTGCGGGCGTA+GGG 0.432905 1_4:-46351444 None:intergenic
TAGAAGTAGAGACGCCAATC+AGG 0.434890 1_4:-46351414 None:intergenic
TCTTCATACGCTCTTGAACT+TGG 0.436566 1_4:-46351557 None:intergenic
TCCAACTAACGATTTAAATC+TGG 0.438023 1_4:+46351804 Msa0165650:CDS
CGCGTACGTGAACAGGATTT+CGG 0.438914 1_4:+46351528 Msa0165650:CDS
GGAAACGAGACGGCGGAGAT+TGG 0.440959 1_4:-46351376 None:intergenic
GGAAGAAGAGATGCTTGAAT+GGG 0.451438 1_4:+46351984 Msa0165650:CDS
ACGGAGTGTGGACCGGGTGC+GGG 0.458435 1_4:-46351451 None:intergenic
ATCAGGGGAGCAGCCTTGGC+CGG 0.460676 1_4:-46351397 None:intergenic
GCTTGAATGGGGAATGTCTC+CGG 0.465250 1_4:+46351996 Msa0165650:CDS
ATATGATTGCTGATCAGAAA+TGG 0.466739 1_4:+46352019 Msa0165650:CDS
GGAGATTGGCGCCGGCGTGG+CGG 0.473230 1_4:-46351362 None:intergenic
TTGAAATTAGAATTCAAAAT+GGG 0.477033 1_4:+46351216 Msa0165650:exon
TTCTGATCAGCAATCATATC+CGG 0.479044 1_4:-46352015 None:intergenic
AACTTCACGGAGTGTGGACC+GGG 0.484362 1_4:-46351457 None:intergenic
GTTAACTGAATGCTGTGGTC+TGG 0.485756 1_4:-46351318 None:intergenic
AAACTGCGAGTTTCGGGTGA+TGG 0.492323 1_4:+46351918 Msa0165650:CDS
GTGGCGGGCTTGTGCGATGC+CGG 0.494843 1_4:-46351346 None:intergenic
AGCATTCAGTTAACTCCATC+CGG 0.508794 1_4:+46351327 Msa0165650:CDS
AGAATACAGTTTGGATGTAT+GGG 0.510305 1_4:-46351074 None:intergenic
CGGGTGATGGAGCAAGGGAG+TGG 0.510953 1_4:+46351931 Msa0165650:CDS
CAGTTTGGCGATTAATCATA+CGG 0.511425 1_4:+46351963 Msa0165650:CDS
ATCGCACAAGCCCGCCACGC+CGG 0.513724 1_4:+46351351 Msa0165650:CDS
TTCTCCAAGAAGCGCGTGAT+CGG 0.514170 1_4:+46351489 Msa0165650:CDS
TCTCTCCCTACGCCCGCACC+CGG 0.515505 1_4:+46351439 Msa0165650:CDS
CAACTTCACGGAGTGTGGAC+CGG 0.516011 1_4:-46351458 None:intergenic
CGGGCTTGTGCGATGCCGGA+TGG 0.517406 1_4:-46351342 None:intergenic
GGCGGAGATTGGCGCCGGCG+TGG 0.518194 1_4:-46351365 None:intergenic
CCAATTTCGATCAGATTACT+CGG 0.523280 1_4:-46352487 None:intergenic
TGTGACCGTCCAATCTCAAT+CGG 0.525925 1_4:+46352404 Msa0165650:three_prime_UTR
GATTACTCGGATCTCAAACT+TGG 0.527329 1_4:-46352474 None:intergenic
AAAACCTATAAATCGAGTAA+AGG 0.531486 1_4:+46351114 Msa0165650:five_prime_UTR
GATGAAGCAATCCAAAATGA+AGG 0.532371 1_4:+46352135 Msa0165650:CDS
AGAAGTAGAGACGCCAATCA+GGG 0.535418 1_4:-46351413 None:intergenic
AATGCTGTGGTCTGGTGTGG+TGG 0.541324 1_4:-46351310 None:intergenic
GAGTTTCGGGTGATGGAGCA+AGG 0.542115 1_4:+46351925 Msa0165650:CDS
GAGTGGTGGAGAATACAGTT+TGG 0.544566 1_4:+46351948 Msa0165650:CDS
GTGATGGAGCAAGGGAGTGG+TGG 0.558690 1_4:+46351934 Msa0165650:CDS
AAGCCATTCTATACTTGTGT+TGG 0.563262 1_4:+46352263 Msa0165650:three_prime_UTR
TCTACCAACTTCACGGAGTG+TGG 0.563838 1_4:-46351463 None:intergenic
GAGACGGCGGAGATTGGCGC+CGG 0.564282 1_4:-46351370 None:intergenic
GATTGGACGGTCACAATGCA+TGG 0.564493 1_4:-46352396 None:intergenic
CTGAATGCTGTGGTCTGGTG+TGG 0.565165 1_4:-46351313 None:intergenic
TGATCATTCCAAGCACCTCT+AGG 0.566827 1_4:-46352054 None:intergenic
ATACCAACACAAGTATAGAA+TGG 0.569770 1_4:-46352266 None:intergenic
CAGATGCGCCTAGAGGTGCT+TGG 0.570632 1_4:+46352046 Msa0165650:CDS
CTTGGAATGATCAATGTCCT+TGG 0.578748 1_4:+46352064 Msa0165650:CDS
TCTTGATGAAGTGGTTCAAG+TGG 0.585341 1_4:+46351860 Msa0165650:CDS
CGGTCCACACTCCGTGAAGT+TGG 0.587452 1_4:+46351459 Msa0165650:CDS
TGGTGTATGCTGACGTGTCA+AGG 0.588203 1_4:-46351290 None:intergenic
ATGCGTCACGGCGAGTCACA+AGG 0.594731 1_4:+46351264 Msa0165650:CDS
AGAAAGATCTACCAACTTCA+CGG 0.598186 1_4:-46351470 None:intergenic
AGCCTTGGCCGGAAACGAGA+CGG 0.598321 1_4:-46351386 None:intergenic
GTAAACCTACAATCAATCAA+AGG 0.599275 1_4:-46352239 None:intergenic
GAAGTAGAGACGCCAATCAG+GGG 0.616210 1_4:-46351412 None:intergenic
GCGATCGAAAACATCGGCGG+CGG 0.622403 1_4:-46351640 None:intergenic
TTGTTAAAGAAACTCGTGAA+CGG 0.625373 1_4:+46351580 Msa0165650:CDS
ACGATCCGATTGAGATTGGA+CGG 0.629679 1_4:-46352409 None:intergenic
ACACATTACAGCTGCCGCCG+CGG 0.637118 1_4:+46351021 Msa0165650:five_prime_UTR
AAATGGGTGTGCTTCCAAAG+AGG 0.650418 1_4:+46351232 Msa0165650:exon
AGTTTCGGGTGATGGAGCAA+GGG 0.652564 1_4:+46351926 Msa0165650:CDS
AATTGATTCTGGTCAAGCCG+CGG 0.657101 1_4:-46351038 None:intergenic
TAATAAGTAACTAATTAAAG+GGG 0.657691 1_4:+46352185 Msa0165650:exon
TGGCGTGCAGATGCGCCTAG+AGG 0.658589 1_4:+46352039 Msa0165650:CDS
GAAGAAGAGATGCTTGAATG+GGG 0.659893 1_4:+46351985 Msa0165650:CDS
TGATTCTGGTCAAGCCGCGG+CGG 0.664767 1_4:-46351035 None:intergenic
AGGATAATCCTAATGCGTCA+CGG 0.674661 1_4:+46351252 Msa0165650:CDS
CTTGGCCGGAAACGAGACGG+CGG 0.676638 1_4:-46351383 None:intergenic
GATGAAGTGGTTCAAGTGGA+CGG 0.691694 1_4:+46351864 Msa0165650:CDS
AGAAATGCGATCGAAAACAT+CGG 0.698994 1_4:-46351646 None:intergenic
ATGGAGTTAACTGAATGCTG+TGG 0.708629 1_4:-46351323 None:intergenic
AATCTGGTGATAGTATCACA+CGG 0.716063 1_4:+46351820 Msa0165650:CDS
GAAGTGGTTCAAGTGGACGG+TGG 0.719720 1_4:+46351867 Msa0165650:CDS
AATGCGATCGAAAACATCGG+CGG 0.731619 1_4:-46351643 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTAATAATCATAATTTA+CGG + chr1_4:46352298-46352317 Msa0165650:three_prime_UTR 10.0%
!! TATGTAATAATCATAATTTA+CGG + chr1_4:46352298-46352317 Msa0165650:three_prime_UTR 10.0%
!! TTGAAATTAGAATTCAAAAT+GGG + chr1_4:46351216-46351235 Msa0165650:exon 15.0%
!! ACTAATAAGTAACTAATTAA+AGG + chr1_4:46352183-46352202 Msa0165650:exon 15.0%
!! CTAATAAGTAACTAATTAAA+GGG + chr1_4:46352184-46352203 Msa0165650:exon 15.0%
!! TAATAAGTAACTAATTAAAG+GGG + chr1_4:46352185-46352204 Msa0165650:exon 15.0%
!!! TATGATTTTCTTCAAAAATT+AGG + chr1_4:46352449-46352468 Msa0165650:three_prime_UTR 15.0%
!! TTGAAATTAGAATTCAAAAT+GGG + chr1_4:46351216-46351235 Msa0165650:exon 15.0%
!! ACTAATAAGTAACTAATTAA+AGG + chr1_4:46352183-46352202 Msa0165650:exon 15.0%
!! CTAATAAGTAACTAATTAAA+GGG + chr1_4:46352184-46352203 Msa0165650:exon 15.0%
!! TAATAAGTAACTAATTAAAG+GGG + chr1_4:46352185-46352204 Msa0165650:exon 15.0%
!!! TATGATTTTCTTCAAAAATT+AGG + chr1_4:46352449-46352468 Msa0165650:three_prime_UTR 15.0%
!! CTTGAAATTAGAATTCAAAA+TGG + chr1_4:46351215-46351234 Msa0165650:exon 20.0%
!! ATTTGAATTTCTTCCTATTT+AGG + chr1_4:46351730-46351749 Msa0165650:intron 20.0%
!! AGATTCAAAAAAACCTAAAT+AGG - chr1_4:46351746-46351765 None:intergenic 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG + chr1_4:46351749-46351768 Msa0165650:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG - chr1_4:46352371-46352390 None:intergenic 20.0%
!! CTTGAAATTAGAATTCAAAA+TGG + chr1_4:46351215-46351234 Msa0165650:exon 20.0%
!! ATTTGAATTTCTTCCTATTT+AGG + chr1_4:46351730-46351749 Msa0165650:intron 20.0%
!! AGATTCAAAAAAACCTAAAT+AGG - chr1_4:46351746-46351765 None:intergenic 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG + chr1_4:46351749-46351768 Msa0165650:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG - chr1_4:46352371-46352390 None:intergenic 20.0%
!!! AAGATTTTGAGAATTGATTC+TGG - chr1_4:46351052-46351071 None:intergenic 25.0%
! AAAACCTATAAATCGAGTAA+AGG + chr1_4:46351114-46351133 Msa0165650:five_prime_UTR 25.0%
!! TTTTCCTTTACTCGATTTAT+AGG - chr1_4:46351121-46351140 None:intergenic 25.0%
!! AAAAAGAAAGTGTGTTAATC+AGG + chr1_4:46351188-46351207 Msa0165650:five_prime_UTR 25.0%
! TTTGAGCATCTAAACAATTT+CGG + chr1_4:46351895-46351914 Msa0165650:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG + chr1_4:46352195-46352214 Msa0165650:three_prime_UTR 25.0%
! AAGGTTATCTTATCATCAAT+TGG - chr1_4:46352223-46352242 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG + chr1_4:46352358-46352377 Msa0165650:three_prime_UTR 25.0%
!!! AAGATTTTGAGAATTGATTC+TGG - chr1_4:46351052-46351071 None:intergenic 25.0%
! AAAACCTATAAATCGAGTAA+AGG + chr1_4:46351114-46351133 Msa0165650:five_prime_UTR 25.0%
!! TTTTCCTTTACTCGATTTAT+AGG - chr1_4:46351121-46351140 None:intergenic 25.0%
!! AAAAAGAAAGTGTGTTAATC+AGG + chr1_4:46351188-46351207 Msa0165650:five_prime_UTR 25.0%
! TTTGAGCATCTAAACAATTT+CGG + chr1_4:46351895-46351914 Msa0165650:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG + chr1_4:46352195-46352214 Msa0165650:three_prime_UTR 25.0%
! AAGGTTATCTTATCATCAAT+TGG - chr1_4:46352223-46352242 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG + chr1_4:46352358-46352377 Msa0165650:three_prime_UTR 25.0%
AGAATACAGTTTGGATGTAT+GGG - chr1_4:46351077-46351096 None:intergenic 30.0%
TAGAATACAGTTTGGATGTA+TGG - chr1_4:46351078-46351097 None:intergenic 30.0%
ACTGTTTGTAGAATACAGTT+TGG - chr1_4:46351086-46351105 None:intergenic 30.0%
! CTTTCTTTTTCTTGATAGGT+TGG - chr1_4:46351178-46351197 None:intergenic 30.0%
! CACACTTTCTTTTTCTTGAT+AGG - chr1_4:46351182-46351201 None:intergenic 30.0%
TTGTTAAAGAAACTCGTGAA+CGG + chr1_4:46351580-46351599 Msa0165650:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG - chr1_4:46351686-46351705 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG + chr1_4:46351804-46351823 Msa0165650:CDS 30.0%
ACCAGATTTAAATCGTTAGT+TGG - chr1_4:46351808-46351827 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG + chr1_4:46352019-46352038 Msa0165650:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG - chr1_4:46352084-46352103 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG - chr1_4:46352149-46352168 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG + chr1_4:46352234-46352253 Msa0165650:three_prime_UTR 30.0%
GTAAACCTACAATCAATCAA+AGG - chr1_4:46352242-46352261 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG - chr1_4:46352269-46352288 None:intergenic 30.0%
AGAATACAGTTTGGATGTAT+GGG - chr1_4:46351077-46351096 None:intergenic 30.0%
TAGAATACAGTTTGGATGTA+TGG - chr1_4:46351078-46351097 None:intergenic 30.0%
ACTGTTTGTAGAATACAGTT+TGG - chr1_4:46351086-46351105 None:intergenic 30.0%
! CTTTCTTTTTCTTGATAGGT+TGG - chr1_4:46351178-46351197 None:intergenic 30.0%
! CACACTTTCTTTTTCTTGAT+AGG - chr1_4:46351182-46351201 None:intergenic 30.0%
TTGTTAAAGAAACTCGTGAA+CGG + chr1_4:46351580-46351599 Msa0165650:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG - chr1_4:46351686-46351705 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG + chr1_4:46351804-46351823 Msa0165650:CDS 30.0%
ACCAGATTTAAATCGTTAGT+TGG - chr1_4:46351808-46351827 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG + chr1_4:46352019-46352038 Msa0165650:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG - chr1_4:46352084-46352103 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG - chr1_4:46352149-46352168 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG + chr1_4:46352234-46352253 Msa0165650:three_prime_UTR 30.0%
GTAAACCTACAATCAATCAA+AGG - chr1_4:46352242-46352261 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG - chr1_4:46352269-46352288 None:intergenic 30.0%
AGAAAGATCTACCAACTTCA+CGG - chr1_4:46351473-46351492 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG + chr1_4:46351612-46351631 Msa0165650:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG - chr1_4:46351649-46351668 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG - chr1_4:46351677-46351696 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG + chr1_4:46351820-46351839 Msa0165650:CDS 35.0%
! CACGAATTTTCTTGATGAAG+TGG + chr1_4:46351851-46351870 Msa0165650:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG + chr1_4:46351963-46351982 Msa0165650:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG - chr1_4:46352018-46352037 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG + chr1_4:46352135-46352154 Msa0165650:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG + chr1_4:46352263-46352282 Msa0165650:three_prime_UTR 35.0%
! CGGATCGTTTAGATTTTAAG+CGG + chr1_4:46352424-46352443 Msa0165650:three_prime_UTR 35.0%
AGAAAGATCTACCAACTTCA+CGG - chr1_4:46351473-46351492 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG + chr1_4:46351612-46351631 Msa0165650:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG - chr1_4:46351649-46351668 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG - chr1_4:46351677-46351696 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG + chr1_4:46351820-46351839 Msa0165650:CDS 35.0%
! CACGAATTTTCTTGATGAAG+TGG + chr1_4:46351851-46351870 Msa0165650:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG + chr1_4:46351963-46351982 Msa0165650:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG - chr1_4:46352018-46352037 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG + chr1_4:46352135-46352154 Msa0165650:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG + chr1_4:46352263-46352282 Msa0165650:three_prime_UTR 35.0%
! CGGATCGTTTAGATTTTAAG+CGG + chr1_4:46352424-46352443 Msa0165650:three_prime_UTR 35.0%
CGCATTAGGATTATCCTCTT+TGG - chr1_4:46351249-46351268 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG + chr1_4:46351252-46351271 Msa0165650:CDS 40.0%
ATGGAGTTAACTGAATGCTG+TGG - chr1_4:46351326-46351345 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG + chr1_4:46351327-46351346 Msa0165650:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG - chr1_4:46351560-46351579 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG + chr1_4:46351585-46351604 Msa0165650:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG + chr1_4:46351586-46351605 Msa0165650:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG + chr1_4:46351648-46351667 Msa0165650:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG + chr1_4:46351860-46351879 Msa0165650:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG + chr1_4:46351911-46351930 Msa0165650:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG + chr1_4:46351984-46352003 Msa0165650:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG + chr1_4:46351985-46352004 Msa0165650:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG + chr1_4:46352064-46352083 Msa0165650:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG + chr1_4:46352075-46352094 Msa0165650:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG - chr1_4:46352416-46352435 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG - chr1_4:46352477-46352496 None:intergenic 40.0%
CGCATTAGGATTATCCTCTT+TGG - chr1_4:46351249-46351268 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG + chr1_4:46351252-46351271 Msa0165650:CDS 40.0%
ATGGAGTTAACTGAATGCTG+TGG - chr1_4:46351326-46351345 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG + chr1_4:46351327-46351346 Msa0165650:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG - chr1_4:46351560-46351579 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG + chr1_4:46351585-46351604 Msa0165650:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG + chr1_4:46351586-46351605 Msa0165650:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG + chr1_4:46351648-46351667 Msa0165650:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG + chr1_4:46351860-46351879 Msa0165650:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG + chr1_4:46351911-46351930 Msa0165650:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG + chr1_4:46351984-46352003 Msa0165650:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG + chr1_4:46351985-46352004 Msa0165650:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG + chr1_4:46352064-46352083 Msa0165650:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG + chr1_4:46352075-46352094 Msa0165650:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG - chr1_4:46352416-46352435 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG - chr1_4:46352477-46352496 None:intergenic 40.0%
! AATTGATTCTGGTCAAGCCG+CGG - chr1_4:46351041-46351060 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG + chr1_4:46351232-46351251 Msa0165650:exon 45.0%
GTTAACTGAATGCTGTGGTC+TGG - chr1_4:46351321-46351340 None:intergenic 45.0%
AGAAGTAGAGACGCCAATCA+GGG - chr1_4:46351416-46351435 None:intergenic 45.0%
TAGAAGTAGAGACGCCAATC+AGG - chr1_4:46351417-46351436 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG - chr1_4:46351646-46351665 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG + chr1_4:46351864-46351883 Msa0165650:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG + chr1_4:46351912-46351931 Msa0165650:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG + chr1_4:46351948-46351967 Msa0165650:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG + chr1_4:46351983-46352002 Msa0165650:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG - chr1_4:46352057-46352076 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG + chr1_4:46352404-46352423 Msa0165650:three_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG - chr1_4:46352412-46352431 None:intergenic 45.0%
CTGTAATGTGTAAGCGAGCA+CGG - chr1_4:46351012-46351031 None:intergenic 45.0%
! AATTGATTCTGGTCAAGCCG+CGG - chr1_4:46351041-46351060 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG + chr1_4:46351232-46351251 Msa0165650:exon 45.0%
GTTAACTGAATGCTGTGGTC+TGG - chr1_4:46351321-46351340 None:intergenic 45.0%
AGAAGTAGAGACGCCAATCA+GGG - chr1_4:46351416-46351435 None:intergenic 45.0%
TAGAAGTAGAGACGCCAATC+AGG - chr1_4:46351417-46351436 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG - chr1_4:46351646-46351665 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG + chr1_4:46351864-46351883 Msa0165650:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG + chr1_4:46351912-46351931 Msa0165650:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG + chr1_4:46351948-46351967 Msa0165650:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG + chr1_4:46351983-46352002 Msa0165650:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG - chr1_4:46352057-46352076 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG + chr1_4:46352404-46352423 Msa0165650:three_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG - chr1_4:46352412-46352431 None:intergenic 45.0%
TGGTGTATGCTGACGTGTCA+AGG - chr1_4:46351293-46351312 None:intergenic 50.0%
GAAGTAGAGACGCCAATCAG+GGG - chr1_4:46351415-46351434 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG - chr1_4:46351466-46351485 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG + chr1_4:46351489-46351508 Msa0165650:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG + chr1_4:46351528-46351547 Msa0165650:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG + chr1_4:46351918-46351937 Msa0165650:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG + chr1_4:46351926-46351945 Msa0165650:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG + chr1_4:46351996-46352015 Msa0165650:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG - chr1_4:46352399-46352418 None:intergenic 50.0%
TGGTGTATGCTGACGTGTCA+AGG - chr1_4:46351293-46351312 None:intergenic 50.0%
GAAGTAGAGACGCCAATCAG+GGG - chr1_4:46351415-46351434 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG - chr1_4:46351466-46351485 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG + chr1_4:46351489-46351508 Msa0165650:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG + chr1_4:46351528-46351547 Msa0165650:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG + chr1_4:46351918-46351937 Msa0165650:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG + chr1_4:46351926-46351945 Msa0165650:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG + chr1_4:46351996-46352015 Msa0165650:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG - chr1_4:46352399-46352418 None:intergenic 50.0%
AATGCTGTGGTCTGGTGTGG+TGG - chr1_4:46351313-46351332 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG - chr1_4:46351316-46351335 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG + chr1_4:46351378-46351397 Msa0165650:CDS 55.0%
AACTTCACGGAGTGTGGACC+GGG - chr1_4:46351460-46351479 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG - chr1_4:46351461-46351480 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG + chr1_4:46351521-46351540 Msa0165650:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG + chr1_4:46351867-46351886 Msa0165650:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG + chr1_4:46351925-46351944 Msa0165650:CDS 55.0%
AATGCTGTGGTCTGGTGTGG+TGG - chr1_4:46351313-46351332 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG - chr1_4:46351316-46351335 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG + chr1_4:46351378-46351397 Msa0165650:CDS 55.0%
AACTTCACGGAGTGTGGACC+GGG - chr1_4:46351460-46351479 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG - chr1_4:46351461-46351480 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG + chr1_4:46351521-46351540 Msa0165650:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG + chr1_4:46351867-46351886 Msa0165650:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG + chr1_4:46351925-46351944 Msa0165650:CDS 55.0%
ACACATTACAGCTGCCGCCG+CGG + chr1_4:46351021-46351040 Msa0165650:five_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG - chr1_4:46351038-46351057 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG - chr1_4:46351263-46351282 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG + chr1_4:46351264-46351283 Msa0165650:CDS 60.0%
GGAAACGAGACGGCGGAGAT+TGG - chr1_4:46351379-46351398 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG - chr1_4:46351389-46351408 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG + chr1_4:46351459-46351478 Msa0165650:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG - chr1_4:46351643-46351662 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG + chr1_4:46351934-46351953 Msa0165650:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG + chr1_4:46352046-46352065 Msa0165650:CDS 60.0%
ACACATTACAGCTGCCGCCG+CGG + chr1_4:46351021-46351040 Msa0165650:five_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG - chr1_4:46351038-46351057 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG - chr1_4:46351263-46351282 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG + chr1_4:46351264-46351283 Msa0165650:CDS 60.0%
GGAAACGAGACGGCGGAGAT+TGG - chr1_4:46351379-46351398 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG - chr1_4:46351389-46351408 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG + chr1_4:46351459-46351478 Msa0165650:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG - chr1_4:46351643-46351662 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG + chr1_4:46351934-46351953 Msa0165650:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG + chr1_4:46352046-46352065 Msa0165650:CDS 60.0%
CTTGGCCGGAAACGAGACGG+CGG - chr1_4:46351386-46351405 None:intergenic 65.0%
ATCAGGGGAGCAGCCTTGGC+CGG - chr1_4:46351400-46351419 None:intergenic 65.0%
GCCAAGGCTGCTCCCCTGAT+TGG + chr1_4:46351400-46351419 Msa0165650:CDS 65.0%
GCCAATCAGGGGAGCAGCCT+TGG - chr1_4:46351404-46351423 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG - chr1_4:46351496-46351515 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG - chr1_4:46351630-46351649 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG + chr1_4:46351931-46351950 Msa0165650:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG + chr1_4:46352039-46352058 Msa0165650:CDS 65.0%
CTTGGCCGGAAACGAGACGG+CGG - chr1_4:46351386-46351405 None:intergenic 65.0%
ATCAGGGGAGCAGCCTTGGC+CGG - chr1_4:46351400-46351419 None:intergenic 65.0%
GCCAAGGCTGCTCCCCTGAT+TGG + chr1_4:46351400-46351419 Msa0165650:CDS 65.0%
GCCAATCAGGGGAGCAGCCT+TGG - chr1_4:46351404-46351423 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG - chr1_4:46351496-46351515 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG - chr1_4:46351630-46351649 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG + chr1_4:46351931-46351950 Msa0165650:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG + chr1_4:46352039-46352058 Msa0165650:CDS 65.0%
CGGGCTTGTGCGATGCCGGA+TGG - chr1_4:46351345-46351364 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG - chr1_4:46351349-46351368 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG + chr1_4:46351351-46351370 Msa0165650:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG - chr1_4:46351373-46351392 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG + chr1_4:46351384-46351403 Msa0165650:CDS 70.0%
TCTCTCCCTACGCCCGCACC+CGG + chr1_4:46351439-46351458 Msa0165650:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG - chr1_4:46351454-46351473 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG - chr1_4:46351455-46351474 None:intergenic 70.0%
CGGGCTTGTGCGATGCCGGA+TGG - chr1_4:46351345-46351364 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG - chr1_4:46351349-46351368 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG + chr1_4:46351351-46351370 Msa0165650:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG - chr1_4:46351373-46351392 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG + chr1_4:46351384-46351403 Msa0165650:CDS 70.0%
TCTCTCCCTACGCCCGCACC+CGG + chr1_4:46351439-46351458 Msa0165650:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG - chr1_4:46351454-46351473 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG - chr1_4:46351455-46351474 None:intergenic 70.0%
!! GAGATTGGCGCCGGCGTGGC+GGG - chr1_4:46351364-46351383 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG - chr1_4:46351365-46351384 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG - chr1_4:46351447-46351466 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG - chr1_4:46351448-46351467 None:intergenic 75.0%
!! GAGATTGGCGCCGGCGTGGC+GGG - chr1_4:46351364-46351383 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG - chr1_4:46351365-46351384 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG - chr1_4:46351447-46351466 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG - chr1_4:46351448-46351467 None:intergenic 75.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG - chr1_4:46351368-46351387 None:intergenic 80.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG - chr1_4:46351368-46351387 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 46351010 46352501 46351010 ID=Msa0165650;Name=Msa0165650
chr1_4 mRNA 46351010 46352501 46351010 ID=Msa0165650-mRNA-1;Parent=Msa0165650;Name=Msa0165650-mRNA-1;_AED=0.02;_eAED=0.02;_QI=224|1|1|1|1|1|2|307|292
chr1_4 exon 46351010 46351669 46351010 ID=Msa0165650-mRNA-1:exon:13298;Parent=Msa0165650-mRNA-1
chr1_4 exon 46351752 46352501 46351752 ID=Msa0165650-mRNA-1:exon:13299;Parent=Msa0165650-mRNA-1
chr1_4 five_prime_UTR 46351010 46351233 46351010 ID=Msa0165650-mRNA-1:five_prime_utr;Parent=Msa0165650-mRNA-1
chr1_4 CDS 46351234 46351669 46351234 ID=Msa0165650-mRNA-1:cds;Parent=Msa0165650-mRNA-1
chr1_4 CDS 46351752 46352194 46351752 ID=Msa0165650-mRNA-1:cds;Parent=Msa0165650-mRNA-1
chr1_4 three_prime_UTR 46352195 46352501 46352195 ID=Msa0165650-mRNA-1:three_prime_utr;Parent=Msa0165650-mRNA-1
Gene Sequence

>Msa0165650

ATGGGTGTGCTTCCAAAGAGGATAATCCTAATGCGTCACGGCGAGTCACAAGGAAACCTTGACACGTCAGCATACACCACCACACCAGACCACAGCATTCAGTTAACTCCATCCGGCATCGCACAAGCCCGCCACGCCGGCGCCAATCTCCGCCGTCTCGTTTCCGGCCAAGGCTGCTCCCCTGATTGGCGTCTCTACTTCTACGTCTCTCCCTACGCCCGCACCCGGTCCACACTCCGTGAAGTTGGTAGATCTTTCTCCAAGAAGCGCGTGATCGGCGTCAGAGAAGAATCGCGCGTACGTGAACAGGATTTCGGTAACTTCCAAGTTCAAGAGCGTATGAAGATTGTTAAAGAAACTCGTGAACGGTTTGGGAGATTCTTTTATCGCTTTCCTGAAGGTGAATCCGCCGCCGATGTTTTCGATCGCATTTCTGGTTTTTTTGAATCTTTATGGAGAGACATTGATTTGAATCGTCTTCATCACGATCCAACTAACGATTTAAATCTGGTGATAGTATCACACGGATTAACATCACGAATTTTCTTGATGAAGTGGTTCAAGTGGACGGTGGAGCAATTTGAGCATCTAAACAATTTCGGAAACTGCGAGTTTCGGGTGATGGAGCAAGGGAGTGGTGGAGAATACAGTTTGGCGATTAATCATACGGAAGAAGAGATGCTTGAATGGGGAATGTCTCCGGATATGATTGCTGATCAGAAATGGCGTGCAGATGCGCCTAGAGGTGCTTGGAATGATCAATGTCCTTGGTATCTTGATGGTTTTTTTGATCGTTTTGCTGAAGATGAATCTGACGATGATGAAGCAATCCAAAATGAAGGTGAAACTGAAATGATAGTTGTTGAAACTAATAAGTAA
Protein sequence

>Msa0165650

MGVLPKRIILMRHGESQGNLDTSAYTTTPDHSIQLTPSGIAQARHAGANLRRLVSGQGCSPDWRLYFYVSPYARTRSTLREVGRSFSKKRVIGVREESRVREQDFGNFQVQERMKIVKETRERFGRFFYRFPEGESAADVFDRISGFFESLWRDIDLNRLHHDPTNDLNLVIVSHGLTSRIFLMKWFKWTVEQFEHLNNFGNCEFRVMEQGSGGEYSLAINHTEEEMLEWGMSPDMIADQKWRADAPRGAWNDQCPWYLDGFFDRFAEDESDDDEAIQNEGETEMIVVETNK*