AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127400 MtrunA17_Chr1g0191411 97.270 293 6 2 1 292 1 292 0.0 586
Msa0127400 MtrunA17_Chr7g0273211 70.877 285 80 1 2 286 34 315 3.16e-156 438
Msa0127400 MtrunA17_Chr5g0397331 52.060 267 124 3 5 269 82 346 1.09e-93 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127400 AT3G05170.1 72.222 270 75 0 3 272 7 276 3.20e-150 423
Msa0127400 AT1G08940.1 68.953 277 80 3 3 275 7 281 4.35e-142 401

Find 105 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGTTTCACCTTCATTT+TGG 0.169991 1_3:+56620177 None:intergenic
TTTCGGAAACTGCGAGTTTC+GGG 0.182047 1_3:-56620411 Msa0127400:CDS
ACTAATAAGTAACTAATTAA+AGG 0.229674 1_3:-56620140 Msa0127400:exon
GTTGGAATTAAAATTCAAAA+TGG 0.245380 1_3:-56621109 Msa0127400:exon
CGCATTAGGATTATCCTCTT+TGG 0.271933 1_3:+56621078 None:intergenic
GACGCCGATCACGCGCTTCT+TGG 0.272814 1_3:+56620831 None:intergenic
CTAATTAAAGGGGAATTTAT+AGG 0.274139 1_3:-56620128 Msa0127400:three_prime_UTR
TTTGAGCATCTAAACAATTT+CGG 0.293790 1_3:-56620428 Msa0127400:CDS
AATCTCCGCCGTCTCGTTTC+CGG 0.313382 1_3:-56620946 Msa0127400:CDS
ACAATCCAGTTTCTGATGTA+TGG 0.328025 1_3:+56621260 None:intergenic
ATTTCGGAAACTGCGAGTTT+CGG 0.331893 1_3:-56620412 Msa0127400:CDS
CGCCGTCTCGTTTCCGGCCA+AGG 0.340582 1_3:-56620940 Msa0127400:CDS
GAGATTGGCGCCGGCGTGGC+GGG 0.342893 1_3:+56620963 None:intergenic
AAGAAACTCGTGAACGGTTT+GGG 0.344466 1_3:-56620738 Msa0127400:CDS
GTGACTCGCCGTGACGCATT+AGG 0.355053 1_3:+56621064 None:intergenic
AGAATCGCGCGTACGTGAAC+AGG 0.355351 1_3:-56620803 Msa0127400:CDS
GATAACCTTTGATTGATTGT+AGG 0.356988 1_3:-56620089 Msa0127400:three_prime_UTR
CTAATAAGTAACTAATTAAA+GGG 0.365006 1_3:-56620139 Msa0127400:exon
TGTGGACCGGGTGCGGGCGT+AGG 0.374827 1_3:+56620879 None:intergenic
AAGGTTATCTTATCATCAAT+TGG 0.379933 1_3:+56620103 None:intergenic
TCGGCGGCGGATTCACCTTC+AGG 0.382270 1_3:+56620697 None:intergenic
CAATGTCCTTGGTATCTTGA+TGG 0.384985 1_3:-56620248 Msa0127400:CDS
AAAGAAACTCGTGAACGGTT+TGG 0.387033 1_3:-56620739 Msa0127400:CDS
GCCAGTCAGGGGAGCAGCCT+TGG 0.397485 1_3:+56620923 None:intergenic
ACCAGATTTAAATCGTTAGT+TGG 0.398319 1_3:+56620518 None:intergenic
GCCAAGGCTGCTCCCCTGAC+TGG 0.403767 1_3:-56620924 Msa0127400:CDS
CACGGAGTGTGGACCGGGTG+CGG 0.410340 1_3:+56620872 None:intergenic
AATCTAATTTCCAAAATTTG+TGG 0.411855 1_3:+56619956 None:intergenic
TATGTAATAATCATAATTTA+CGG 0.419568 1_3:-56620025 Msa0127400:three_prime_UTR
CGGAAGAAGAGATGCTTGAA+TGG 0.421203 1_3:-56620340 Msa0127400:CDS
TAGAAGTAGAGACGCCAGTC+AGG 0.422292 1_3:+56620910 None:intergenic
CTAAACGATCCGATTGAGAT+TGG 0.432151 1_3:+56619911 None:intergenic
GTGGACCGGGTGCGGGCGTA+GGG 0.432905 1_3:+56620880 None:intergenic
TCTTCATACGCTCTTGAACT+TGG 0.436566 1_3:+56620767 None:intergenic
GTCAGGGGAGCAGCCTTGGC+CGG 0.437420 1_3:+56620927 None:intergenic
TCCAACTAACGATTTAAATC+TGG 0.438023 1_3:-56620519 Msa0127400:CDS
CGCGTACGTGAACAGGATTT+CGG 0.438914 1_3:-56620796 Msa0127400:CDS
GGAAACGAGACGGCGGAGAT+TGG 0.440959 1_3:+56620948 None:intergenic
TTGGAATTAAAATTCAAAAT+GGG 0.446468 1_3:-56621108 Msa0127400:exon
GGAAGAAGAGATGCTTGAAT+GGG 0.451438 1_3:-56620339 Msa0127400:CDS
ACGGAGTGTGGACCGGGTGC+GGG 0.458435 1_3:+56620873 None:intergenic
GCTTGAATGGGGAATGTCTC+CGG 0.465250 1_3:-56620327 Msa0127400:CDS
ATATGATTGCTGATCAGAAA+TGG 0.466739 1_3:-56620304 Msa0127400:CDS
GGAGATTGGCGCCGGCGTGG+CGG 0.473230 1_3:+56620962 None:intergenic
TTCTGATCAGCAATCATATC+CGG 0.479044 1_3:+56620308 None:intergenic
AACTTCACGGAGTGTGGACC+GGG 0.484362 1_3:+56620867 None:intergenic
GTTAACTGAATGCTGTGGTC+TGG 0.485756 1_3:+56621006 None:intergenic
AAACTGCGAGTTTCGGGTGA+TGG 0.492323 1_3:-56620405 Msa0127400:CDS
GTGGCGGGCTTGTGCGATGC+CGG 0.494843 1_3:+56620978 None:intergenic
CAATCCAGTTTCTGATGTAT+GGG 0.498455 1_3:+56621261 None:intergenic
AGCATTCAGTTAACTCCATC+CGG 0.508794 1_3:-56620997 Msa0127400:CDS
CGGGTGATGGAGCAAGGGAG+TGG 0.510953 1_3:-56620392 Msa0127400:CDS
CAGTTTGGCGATTAATCATA+CGG 0.511425 1_3:-56620360 Msa0127400:CDS
ATCGCACAAGCCCGCCACGC+CGG 0.513724 1_3:-56620973 Msa0127400:CDS
TTCTCCAAGAAGCGCGTGAT+CGG 0.514170 1_3:-56620835 Msa0127400:CDS
TCTCTCCCTACGCCCGCACC+CGG 0.515505 1_3:-56620885 Msa0127400:CDS
CAACTTCACGGAGTGTGGAC+CGG 0.516011 1_3:+56620866 None:intergenic
CGGGCTTGTGCGATGCCGGA+TGG 0.517406 1_3:+56620982 None:intergenic
GGCGGAGATTGGCGCCGGCG+TGG 0.518194 1_3:+56620959 None:intergenic
CCAATTTCGATCAGATTACT+CGG 0.523280 1_3:+56619837 None:intergenic
TGTGACCGTCCAATCTCAAT+CGG 0.525925 1_3:-56619920 Msa0127400:three_prime_UTR
GATTACTCGGATCTCAAACT+TGG 0.527329 1_3:+56619850 None:intergenic
GATGAAGCAATCCAAAATGA+AGG 0.532371 1_3:-56620188 Msa0127400:CDS
AATGCTGTGGTCTGGTGTGG+TGG 0.541324 1_3:+56621014 None:intergenic
GAGTTTCGGGTGATGGAGCA+AGG 0.542115 1_3:-56620398 Msa0127400:CDS
GAGTGGTGGAGAATACAGTT+TGG 0.544566 1_3:-56620375 Msa0127400:CDS
ATACCAACACAAGTATAGAA+TGG 0.553757 1_3:+56620057 None:intergenic
AGAAGTAGAGACGCCAGTCA+GGG 0.558329 1_3:+56620911 None:intergenic
GTGATGGAGCAAGGGAGTGG+TGG 0.558690 1_3:-56620389 Msa0127400:CDS
AAGCCATTCTATACTTGTGT+TGG 0.563262 1_3:-56620060 Msa0127400:three_prime_UTR
TCTACCAACTTCACGGAGTG+TGG 0.563838 1_3:+56620861 None:intergenic
GAGACGGCGGAGATTGGCGC+CGG 0.564282 1_3:+56620954 None:intergenic
GATTGGACGGTCACAATGCA+TGG 0.564493 1_3:+56619928 None:intergenic
CTGAATGCTGTGGTCTGGTG+TGG 0.565165 1_3:+56621011 None:intergenic
TGATCATTCCAAGCACCTCT+AGG 0.566827 1_3:+56620269 None:intergenic
CAGATGCGCCTAGAGGTGCT+TGG 0.570632 1_3:-56620277 Msa0127400:CDS
CTTGGAATGATCAATGTCCT+TGG 0.578748 1_3:-56620259 Msa0127400:CDS
TCTTGATGAAGTGGTTCAAG+TGG 0.585341 1_3:-56620463 Msa0127400:CDS
CGGTCCACACTCCGTGAAGT+TGG 0.587452 1_3:-56620865 Msa0127400:CDS
TGGTGTATGCTGACGTGTCA+AGG 0.588203 1_3:+56621034 None:intergenic
ATGCGTCACGGCGAGTCACA+AGG 0.594731 1_3:-56621060 Msa0127400:CDS
AGAAAGATCTACCAACTTCA+CGG 0.598186 1_3:+56620854 None:intergenic
AGCCTTGGCCGGAAACGAGA+CGG 0.598321 1_3:+56620938 None:intergenic
GTAAACCTACAATCAATCAA+AGG 0.599275 1_3:+56620084 None:intergenic
GAAGTAGAGACGCCAGTCAG+GGG 0.613104 1_3:+56620912 None:intergenic
GCGATCGAAAACATCGGCGG+CGG 0.622403 1_3:+56620684 None:intergenic
TTGTTAAAGAAACTCGTGAA+CGG 0.625373 1_3:-56620744 Msa0127400:CDS
ACGATCCGATTGAGATTGGA+CGG 0.629679 1_3:+56619915 None:intergenic
ACACATTACAGCTGCCGCCG+CGG 0.637118 1_3:-56621314 Msa0127400:five_prime_UTR
AAATGGGTGTGCTTCCAAAG+AGG 0.650418 1_3:-56621092 Msa0127400:CDS
AGTTTCGGGTGATGGAGCAA+GGG 0.652564 1_3:-56620397 Msa0127400:CDS
AAATGATTCTGGTCAAGCCG+CGG 0.655794 1_3:+56621297 None:intergenic
TAATAAGTAACTAATTAAAG+GGG 0.657691 1_3:-56620138 Msa0127400:exon
TGGCGTGCAGATGCGCCTAG+AGG 0.658589 1_3:-56620284 Msa0127400:CDS
GAAGAAGAGATGCTTGAATG+GGG 0.659893 1_3:-56620338 Msa0127400:CDS
TGATTCTGGTCAAGCCGCGG+CGG 0.666786 1_3:+56621300 None:intergenic
AGGATAATCCTAATGCGTCA+CGG 0.674661 1_3:-56621072 Msa0127400:CDS
CTTGGCCGGAAACGAGACGG+CGG 0.676638 1_3:+56620941 None:intergenic
GATGAAGTGGTTCAAGTGGA+CGG 0.691694 1_3:-56620459 Msa0127400:CDS
GCAGCTGTAATGTGTAAGCG+AGG 0.691916 1_3:+56621322 None:intergenic
AGAAATGCGATCGAAAACAT+CGG 0.698994 1_3:+56620678 None:intergenic
ATGGAGTTAACTGAATGCTG+TGG 0.708629 1_3:+56621001 None:intergenic
AATCTGGTGATAGTATCACA+CGG 0.716063 1_3:-56620503 Msa0127400:CDS
GAAGTGGTTCAAGTGGACGG+TGG 0.719720 1_3:-56620456 Msa0127400:CDS
AATGCGATCGAAAACATCGG+CGG 0.731619 1_3:+56620681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTAATAATCATAATTTA+CGG - chr1_3:56621145-56621164 Msa0127400:five_prime_UTR 10.0%
!! TATGTAATAATCATAATTTA+CGG - chr1_3:56621145-56621164 Msa0127400:five_prime_UTR 10.0%
!! TTGGAATTAAAATTCAAAAT+GGG - chr1_3:56620062-56620081 Msa0127400:three_prime_UTR 15.0%
!! ACTAATAAGTAACTAATTAA+AGG - chr1_3:56621030-56621049 Msa0127400:CDS 15.0%
!! CTAATAAGTAACTAATTAAA+GGG - chr1_3:56621031-56621050 Msa0127400:CDS 15.0%
!! TAATAAGTAACTAATTAAAG+GGG - chr1_3:56621032-56621051 Msa0127400:CDS 15.0%
!!! TTTGATTTTCTTCAAAAATT+AGG - chr1_3:56621295-56621314 Msa0127400:five_prime_UTR 15.0%
!! TTGGAATTAAAATTCAAAAT+GGG - chr1_3:56620062-56620081 Msa0127400:three_prime_UTR 15.0%
!! ACTAATAAGTAACTAATTAA+AGG - chr1_3:56621030-56621049 Msa0127400:CDS 15.0%
!! CTAATAAGTAACTAATTAAA+GGG - chr1_3:56621031-56621050 Msa0127400:CDS 15.0%
!! TAATAAGTAACTAATTAAAG+GGG - chr1_3:56621032-56621051 Msa0127400:CDS 15.0%
!!! TTTGATTTTCTTCAAAAATT+AGG - chr1_3:56621295-56621314 Msa0127400:five_prime_UTR 15.0%
!!! TTTTGATTTACTCGATTTAT+AGG + chr1_3:56619964-56619983 None:intergenic 20.0%
!!! AAAAAGAAAGTGTTTTGATT+AGG - chr1_3:56620031-56620050 Msa0127400:three_prime_UTR 20.0%
!! GTTGGAATTAAAATTCAAAA+TGG - chr1_3:56620061-56620080 Msa0127400:three_prime_UTR 20.0%
!! ATTTGAATTTCTTCCTATTT+AGG - chr1_3:56620577-56620596 Msa0127400:intron 20.0%
!! AGATTCAAAAAAACCTAAAT+AGG + chr1_3:56620593-56620612 None:intergenic 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG - chr1_3:56620596-56620615 Msa0127400:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG + chr1_3:56621217-56621236 None:intergenic 20.0%
!!! TTTTGATTTACTCGATTTAT+AGG + chr1_3:56619964-56619983 None:intergenic 20.0%
!!! AAAAAGAAAGTGTTTTGATT+AGG - chr1_3:56620031-56620050 Msa0127400:three_prime_UTR 20.0%
!! GTTGGAATTAAAATTCAAAA+TGG - chr1_3:56620061-56620080 Msa0127400:three_prime_UTR 20.0%
!! ATTTGAATTTCTTCCTATTT+AGG - chr1_3:56620577-56620596 Msa0127400:intron 20.0%
!! AGATTCAAAAAAACCTAAAT+AGG + chr1_3:56620593-56620612 None:intergenic 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG - chr1_3:56620596-56620615 Msa0127400:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG + chr1_3:56621217-56621236 None:intergenic 20.0%
!!! AAGATTTTGAGAAATGATTC+TGG + chr1_3:56619887-56619906 None:intergenic 25.0%
!! CTTTCTTTTTCTTGATATGT+TGG + chr1_3:56620021-56620040 None:intergenic 25.0%
! TTTGAGCATCTAAACAATTT+CGG - chr1_3:56620742-56620761 Msa0127400:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG - chr1_3:56621042-56621061 Msa0127400:CDS 25.0%
! AAGGTTATCTTATCATCAAT+TGG + chr1_3:56621070-56621089 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG - chr1_3:56621204-56621223 Msa0127400:five_prime_UTR 25.0%
!!! AAGATTTTGAGAAATGATTC+TGG + chr1_3:56619887-56619906 None:intergenic 25.0%
!! CTTTCTTTTTCTTGATATGT+TGG + chr1_3:56620021-56620040 None:intergenic 25.0%
! TTTGAGCATCTAAACAATTT+CGG - chr1_3:56620742-56620761 Msa0127400:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG - chr1_3:56621042-56621061 Msa0127400:CDS 25.0%
! AAGGTTATCTTATCATCAAT+TGG + chr1_3:56621070-56621089 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG - chr1_3:56621204-56621223 Msa0127400:five_prime_UTR 25.0%
!! TTTTGATTAGGAGAACTTGT+TGG - chr1_3:56620043-56620062 Msa0127400:three_prime_UTR 30.0%
TTGTTAAAGAAACTCGTGAA+CGG - chr1_3:56620426-56620445 Msa0127400:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG + chr1_3:56620532-56620551 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG - chr1_3:56620651-56620670 Msa0127400:intron 30.0%
ACCAGATTTAAATCGTTAGT+TGG + chr1_3:56620655-56620674 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG - chr1_3:56620866-56620885 Msa0127400:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG + chr1_3:56620931-56620950 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG + chr1_3:56620996-56621015 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG - chr1_3:56621081-56621100 Msa0127400:CDS 30.0%
GTAAACCTACAATCAATCAA+AGG + chr1_3:56621089-56621108 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG + chr1_3:56621116-56621135 None:intergenic 30.0%
!! TTTTGATTAGGAGAACTTGT+TGG - chr1_3:56620043-56620062 Msa0127400:three_prime_UTR 30.0%
TTGTTAAAGAAACTCGTGAA+CGG - chr1_3:56620426-56620445 Msa0127400:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG + chr1_3:56620532-56620551 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG - chr1_3:56620651-56620670 Msa0127400:intron 30.0%
ACCAGATTTAAATCGTTAGT+TGG + chr1_3:56620655-56620674 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG - chr1_3:56620866-56620885 Msa0127400:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG + chr1_3:56620931-56620950 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG + chr1_3:56620996-56621015 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG - chr1_3:56621081-56621100 Msa0127400:CDS 30.0%
GTAAACCTACAATCAATCAA+AGG + chr1_3:56621089-56621108 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG + chr1_3:56621116-56621135 None:intergenic 30.0%
! TTTTCCCATACATCAGAAAC+TGG - chr1_3:56619905-56619924 Msa0127400:three_prime_UTR 35.0%
CAATCCAGTTTCTGATGTAT+GGG + chr1_3:56619912-56619931 None:intergenic 35.0%
ACAATCCAGTTTCTGATGTA+TGG + chr1_3:56619913-56619932 None:intergenic 35.0%
AGAAAGATCTACCAACTTCA+CGG + chr1_3:56620319-56620338 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG - chr1_3:56620458-56620477 Msa0127400:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG + chr1_3:56620495-56620514 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG + chr1_3:56620523-56620542 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG - chr1_3:56620667-56620686 Msa0127400:intron 35.0%
! CACGAATTTTCTTGATGAAG+TGG - chr1_3:56620698-56620717 Msa0127400:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG - chr1_3:56620810-56620829 Msa0127400:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG + chr1_3:56620865-56620884 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG - chr1_3:56620982-56621001 Msa0127400:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG - chr1_3:56621110-56621129 Msa0127400:exon 35.0%
! CGGATCGTTTAGATTTTAAG+CGG - chr1_3:56621270-56621289 Msa0127400:five_prime_UTR 35.0%
! TTTTCCCATACATCAGAAAC+TGG - chr1_3:56619905-56619924 Msa0127400:three_prime_UTR 35.0%
CAATCCAGTTTCTGATGTAT+GGG + chr1_3:56619912-56619931 None:intergenic 35.0%
ACAATCCAGTTTCTGATGTA+TGG + chr1_3:56619913-56619932 None:intergenic 35.0%
AGAAAGATCTACCAACTTCA+CGG + chr1_3:56620319-56620338 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG - chr1_3:56620458-56620477 Msa0127400:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG + chr1_3:56620495-56620514 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG + chr1_3:56620523-56620542 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG - chr1_3:56620667-56620686 Msa0127400:intron 35.0%
! CACGAATTTTCTTGATGAAG+TGG - chr1_3:56620698-56620717 Msa0127400:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG - chr1_3:56620810-56620829 Msa0127400:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG + chr1_3:56620865-56620884 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG - chr1_3:56620982-56621001 Msa0127400:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG - chr1_3:56621110-56621129 Msa0127400:exon 35.0%
! CGGATCGTTTAGATTTTAAG+CGG - chr1_3:56621270-56621289 Msa0127400:five_prime_UTR 35.0%
CGCATTAGGATTATCCTCTT+TGG + chr1_3:56620095-56620114 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG - chr1_3:56620098-56620117 Msa0127400:three_prime_UTR 40.0%
ATGGAGTTAACTGAATGCTG+TGG + chr1_3:56620172-56620191 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG - chr1_3:56620173-56620192 Msa0127400:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG + chr1_3:56620406-56620425 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG - chr1_3:56620431-56620450 Msa0127400:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG - chr1_3:56620432-56620451 Msa0127400:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG - chr1_3:56620494-56620513 Msa0127400:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG - chr1_3:56620707-56620726 Msa0127400:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG - chr1_3:56620758-56620777 Msa0127400:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG - chr1_3:56620831-56620850 Msa0127400:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG - chr1_3:56620832-56620851 Msa0127400:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG - chr1_3:56620911-56620930 Msa0127400:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG - chr1_3:56620922-56620941 Msa0127400:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG + chr1_3:56621262-56621281 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG + chr1_3:56621323-56621342 None:intergenic 40.0%
CGCATTAGGATTATCCTCTT+TGG + chr1_3:56620095-56620114 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG - chr1_3:56620098-56620117 Msa0127400:three_prime_UTR 40.0%
ATGGAGTTAACTGAATGCTG+TGG + chr1_3:56620172-56620191 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG - chr1_3:56620173-56620192 Msa0127400:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG + chr1_3:56620406-56620425 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG - chr1_3:56620431-56620450 Msa0127400:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG - chr1_3:56620432-56620451 Msa0127400:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG - chr1_3:56620494-56620513 Msa0127400:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG - chr1_3:56620707-56620726 Msa0127400:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG - chr1_3:56620758-56620777 Msa0127400:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG - chr1_3:56620831-56620850 Msa0127400:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG - chr1_3:56620832-56620851 Msa0127400:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG - chr1_3:56620911-56620930 Msa0127400:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG - chr1_3:56620922-56620941 Msa0127400:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG + chr1_3:56621262-56621281 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG + chr1_3:56621323-56621342 None:intergenic 40.0%
AAATGATTCTGGTCAAGCCG+CGG + chr1_3:56619876-56619895 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG - chr1_3:56620078-56620097 Msa0127400:three_prime_UTR 45.0%
GTTAACTGAATGCTGTGGTC+TGG + chr1_3:56620167-56620186 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG + chr1_3:56620492-56620511 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG - chr1_3:56620711-56620730 Msa0127400:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG - chr1_3:56620759-56620778 Msa0127400:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG - chr1_3:56620795-56620814 Msa0127400:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG - chr1_3:56620830-56620849 Msa0127400:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG + chr1_3:56620904-56620923 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG - chr1_3:56621250-56621269 Msa0127400:five_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG + chr1_3:56621258-56621277 None:intergenic 45.0%
CTGTAATGTGTAAGCGAGGA+CGG + chr1_3:56619847-56619866 None:intergenic 45.0%
AAATGATTCTGGTCAAGCCG+CGG + chr1_3:56619876-56619895 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG - chr1_3:56620078-56620097 Msa0127400:three_prime_UTR 45.0%
GTTAACTGAATGCTGTGGTC+TGG + chr1_3:56620167-56620186 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG + chr1_3:56620492-56620511 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG - chr1_3:56620711-56620730 Msa0127400:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG - chr1_3:56620759-56620778 Msa0127400:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG - chr1_3:56620795-56620814 Msa0127400:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG - chr1_3:56620830-56620849 Msa0127400:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG + chr1_3:56620904-56620923 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG - chr1_3:56621250-56621269 Msa0127400:five_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG + chr1_3:56621258-56621277 None:intergenic 45.0%
GCAGCTGTAATGTGTAAGCG+AGG + chr1_3:56619851-56619870 None:intergenic 50.0%
TGGTGTATGCTGACGTGTCA+AGG + chr1_3:56620139-56620158 None:intergenic 50.0%
AGAAGTAGAGACGCCAGTCA+GGG + chr1_3:56620262-56620281 None:intergenic 50.0%
TAGAAGTAGAGACGCCAGTC+AGG + chr1_3:56620263-56620282 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG + chr1_3:56620312-56620331 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG - chr1_3:56620335-56620354 Msa0127400:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG - chr1_3:56620374-56620393 Msa0127400:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG - chr1_3:56620765-56620784 Msa0127400:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG - chr1_3:56620773-56620792 Msa0127400:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG - chr1_3:56620843-56620862 Msa0127400:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG + chr1_3:56621245-56621264 None:intergenic 50.0%
GCAGCTGTAATGTGTAAGCG+AGG + chr1_3:56619851-56619870 None:intergenic 50.0%
TGGTGTATGCTGACGTGTCA+AGG + chr1_3:56620139-56620158 None:intergenic 50.0%
AGAAGTAGAGACGCCAGTCA+GGG + chr1_3:56620262-56620281 None:intergenic 50.0%
TAGAAGTAGAGACGCCAGTC+AGG + chr1_3:56620263-56620282 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG + chr1_3:56620312-56620331 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG - chr1_3:56620335-56620354 Msa0127400:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG - chr1_3:56620374-56620393 Msa0127400:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG - chr1_3:56620765-56620784 Msa0127400:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG - chr1_3:56620773-56620792 Msa0127400:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG - chr1_3:56620843-56620862 Msa0127400:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG + chr1_3:56621245-56621264 None:intergenic 50.0%
AATGCTGTGGTCTGGTGTGG+TGG + chr1_3:56620159-56620178 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG + chr1_3:56620162-56620181 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG - chr1_3:56620224-56620243 Msa0127400:CDS 55.0%
GAAGTAGAGACGCCAGTCAG+GGG + chr1_3:56620261-56620280 None:intergenic 55.0%
AACTTCACGGAGTGTGGACC+GGG + chr1_3:56620306-56620325 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG + chr1_3:56620307-56620326 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG - chr1_3:56620367-56620386 Msa0127400:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG - chr1_3:56620714-56620733 Msa0127400:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG - chr1_3:56620772-56620791 Msa0127400:CDS 55.0%
AATGCTGTGGTCTGGTGTGG+TGG + chr1_3:56620159-56620178 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG + chr1_3:56620162-56620181 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG - chr1_3:56620224-56620243 Msa0127400:CDS 55.0%
GAAGTAGAGACGCCAGTCAG+GGG + chr1_3:56620261-56620280 None:intergenic 55.0%
AACTTCACGGAGTGTGGACC+GGG + chr1_3:56620306-56620325 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG + chr1_3:56620307-56620326 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG - chr1_3:56620367-56620386 Msa0127400:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG - chr1_3:56620714-56620733 Msa0127400:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG - chr1_3:56620772-56620791 Msa0127400:CDS 55.0%
ACACATTACAGCTGCCGCCG+CGG - chr1_3:56619856-56619875 Msa0127400:three_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG + chr1_3:56619873-56619892 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG + chr1_3:56620109-56620128 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG - chr1_3:56620110-56620129 Msa0127400:three_prime_UTR 60.0%
GGAAACGAGACGGCGGAGAT+TGG + chr1_3:56620225-56620244 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG + chr1_3:56620235-56620254 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG - chr1_3:56620305-56620324 Msa0127400:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG + chr1_3:56620489-56620508 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG - chr1_3:56620781-56620800 Msa0127400:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG - chr1_3:56620893-56620912 Msa0127400:CDS 60.0%
ACACATTACAGCTGCCGCCG+CGG - chr1_3:56619856-56619875 Msa0127400:three_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG + chr1_3:56619873-56619892 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG + chr1_3:56620109-56620128 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG - chr1_3:56620110-56620129 Msa0127400:three_prime_UTR 60.0%
GGAAACGAGACGGCGGAGAT+TGG + chr1_3:56620225-56620244 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG + chr1_3:56620235-56620254 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG - chr1_3:56620305-56620324 Msa0127400:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG + chr1_3:56620489-56620508 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG - chr1_3:56620781-56620800 Msa0127400:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG - chr1_3:56620893-56620912 Msa0127400:CDS 60.0%
CTTGGCCGGAAACGAGACGG+CGG + chr1_3:56620232-56620251 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG + chr1_3:56620342-56620361 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG + chr1_3:56620476-56620495 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG - chr1_3:56620778-56620797 Msa0127400:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG - chr1_3:56620886-56620905 Msa0127400:CDS 65.0%
CTTGGCCGGAAACGAGACGG+CGG + chr1_3:56620232-56620251 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG + chr1_3:56620342-56620361 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG + chr1_3:56620476-56620495 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG - chr1_3:56620778-56620797 Msa0127400:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG - chr1_3:56620886-56620905 Msa0127400:CDS 65.0%
CGGGCTTGTGCGATGCCGGA+TGG + chr1_3:56620191-56620210 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG + chr1_3:56620195-56620214 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG - chr1_3:56620197-56620216 Msa0127400:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG + chr1_3:56620219-56620238 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG - chr1_3:56620230-56620249 Msa0127400:CDS 70.0%
GTCAGGGGAGCAGCCTTGGC+CGG + chr1_3:56620246-56620265 None:intergenic 70.0%
GCCAAGGCTGCTCCCCTGAC+TGG - chr1_3:56620246-56620265 Msa0127400:CDS 70.0%
GCCAGTCAGGGGAGCAGCCT+TGG + chr1_3:56620250-56620269 None:intergenic 70.0%
TCTCTCCCTACGCCCGCACC+CGG - chr1_3:56620285-56620304 Msa0127400:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG + chr1_3:56620300-56620319 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG + chr1_3:56620301-56620320 None:intergenic 70.0%
CGGGCTTGTGCGATGCCGGA+TGG + chr1_3:56620191-56620210 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG + chr1_3:56620195-56620214 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG - chr1_3:56620197-56620216 Msa0127400:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG + chr1_3:56620219-56620238 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG - chr1_3:56620230-56620249 Msa0127400:CDS 70.0%
GTCAGGGGAGCAGCCTTGGC+CGG + chr1_3:56620246-56620265 None:intergenic 70.0%
GCCAAGGCTGCTCCCCTGAC+TGG - chr1_3:56620246-56620265 Msa0127400:CDS 70.0%
GCCAGTCAGGGGAGCAGCCT+TGG + chr1_3:56620250-56620269 None:intergenic 70.0%
TCTCTCCCTACGCCCGCACC+CGG - chr1_3:56620285-56620304 Msa0127400:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG + chr1_3:56620300-56620319 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG + chr1_3:56620301-56620320 None:intergenic 70.0%
!! GAGATTGGCGCCGGCGTGGC+GGG + chr1_3:56620210-56620229 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG + chr1_3:56620211-56620230 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG + chr1_3:56620293-56620312 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG + chr1_3:56620294-56620313 None:intergenic 75.0%
!! GAGATTGGCGCCGGCGTGGC+GGG + chr1_3:56620210-56620229 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG + chr1_3:56620211-56620230 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG + chr1_3:56620293-56620312 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG + chr1_3:56620294-56620313 None:intergenic 75.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG + chr1_3:56620214-56620233 None:intergenic 80.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG + chr1_3:56620214-56620233 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 56619845 56621347 56619845 ID=Msa0127400;Name=Msa0127400
chr1_3 mRNA 56619845 56621347 56619845 ID=Msa0127400-mRNA-1;Parent=Msa0127400;Name=Msa0127400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=235|1|1|1|1|1|2|306|292
chr1_3 exon 56619845 56620593 56619845 ID=Msa0127400-mRNA-1:exon:17951;Parent=Msa0127400-mRNA-1
chr1_3 exon 56620677 56621347 56620677 ID=Msa0127400-mRNA-1:exon:17950;Parent=Msa0127400-mRNA-1
chr1_3 five_prime_UTR 56621113 56621347 56621113 ID=Msa0127400-mRNA-1:five_prime_utr;Parent=Msa0127400-mRNA-1
chr1_3 CDS 56620677 56621112 56620677 ID=Msa0127400-mRNA-1:cds;Parent=Msa0127400-mRNA-1
chr1_3 CDS 56620151 56620593 56620151 ID=Msa0127400-mRNA-1:cds;Parent=Msa0127400-mRNA-1
chr1_3 three_prime_UTR 56619845 56620150 56619845 ID=Msa0127400-mRNA-1:three_prime_utr;Parent=Msa0127400-mRNA-1
Gene Sequence

>Msa0127400

ATGGGTGTGCTTCCAAAGAGGATAATCCTAATGCGTCACGGCGAGTCACAAGGAAACCTTGACACGTCAGCATACACCACCACACCAGACCACAGCATTCAGTTAACTCCATCCGGCATCGCACAAGCCCGCCACGCCGGCGCCAATCTCCGCCGTCTCGTTTCCGGCCAAGGCTGCTCCCCTGACTGGCGTCTCTACTTCTACGTCTCTCCCTACGCCCGCACCCGGTCCACACTCCGTGAAGTTGGTAGATCTTTCTCCAAGAAGCGCGTGATCGGCGTCAGAGAAGAATCGCGCGTACGTGAACAGGATTTCGGTAACTTCCAAGTTCAAGAGCGTATGAAGATTGTTAAAGAAACTCGTGAACGGTTTGGGAGATTCTTTTATCGCTTTCCTGAAGGTGAATCCGCCGCCGATGTTTTCGATCGCATTTCTGGTTTTTTTGAATCTTTATGGAGAGACATTGATTTGAATCGTCTTCATCACGATCCAACTAACGATTTAAATCTGGTGATAGTATCACACGGATTAACATCACGAATTTTCTTGATGAAGTGGTTCAAGTGGACGGTGGAGCAATTTGAGCATCTAAACAATTTCGGAAACTGCGAGTTTCGGGTGATGGAGCAAGGGAGTGGTGGAGAATACAGTTTGGCGATTAATCATACGGAAGAAGAGATGCTTGAATGGGGAATGTCTCCGGATATGATTGCTGATCAGAAATGGCGTGCAGATGCGCCTAGAGGTGCTTGGAATGATCAATGTCCTTGGTATCTTGATGGTTTTTTTGATCGTTTTGCTGAAGATGAATCTGACGATGATGAAGCAATCCAAAATGAAGGTGAAACTGAAATGATAGTTGTTGAAACTAATAAGTAA
Protein sequence

>Msa0127400

MGVLPKRIILMRHGESQGNLDTSAYTTTPDHSIQLTPSGIAQARHAGANLRRLVSGQGCSPDWRLYFYVSPYARTRSTLREVGRSFSKKRVIGVREESRVREQDFGNFQVQERMKIVKETRERFGRFFYRFPEGESAADVFDRISGFFESLWRDIDLNRLHHDPTNDLNLVIVSHGLTSRIFLMKWFKWTVEQFEHLNNFGNCEFRVMEQGSGGEYSLAINHTEEEMLEWGMSPDMIADQKWRADAPRGAWNDQCPWYLDGFFDRFAEDESDDDEAIQNEGETEMIVVETNK*