AlfalfaGEDB | Msa0127400

M. sativa cultivar zhongmu-4 / Msa0127400

KEGG: -
GO: GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0071704
PFAM: PF00300.25

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127400	sp\|Q9MAA2\|AT74_ARATH	72.222	270	75	0	3	272	7	276	3.15e-149	423

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127400	G7I7H1	97.270	293	6	2	1	292	1	292	0.0	586

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0033140	Msa0127400	0.966671	3.112241e-126	-8.615850e-47
Msa0037180	Msa0127400	0.810984	8.780545e-51	-8.615850e-47
Msa0079810	Msa0127400	0.883707	3.573052e-71	-8.615850e-47
Msa0092220	Msa0127400	0.807215	5.636026e-50	-8.615850e-47
Msa0127400	Msa0165650	0.893876	4.178399e-75	-8.615850e-47
Msa0127400	Msa0177320	0.815184	1.053081e-51	-8.615850e-47
Msa0127400	Msa0266220	0.803843	2.872228e-49	-8.615850e-47
Msa0127400	Msa0287680	0.823462	1.367507e-53	-8.615850e-47
Msa0127400	Msa0888980	0.817327	3.492043e-52	-8.615850e-47
Msa0127400	Msa1350700	0.822306	2.542316e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127400	MtrunA17_Chr1g0191411	97.270	293	6	2	1	292	1	292	0.0	586
Msa0127400	MtrunA17_Chr7g0273211	70.877	285	80	1	2	286	34	315	3.16e-156	438
Msa0127400	MtrunA17_Chr5g0397331	52.060	267	124	3	5	269	82	346	1.09e-93	281

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0127400	AT3G05170.1	72.222	270	75	0	3	272	7	276	3.20e-150	423
Msa0127400	AT1G08940.1	68.953	277	80	3	3	275	7	281	4.35e-142	401

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Find 105 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTGCGAGTTTCGGGTGA+TGG	0.492323	1_3:-56620405	Msa0127400:CDS
AAAGAAACTCGTGAACGGTT+TGG	0.387033	1_3:-56620739	Msa0127400:CDS
AAATGATTCTGGTCAAGCCG+CGG	0.655794	1_3:+56621297	None:intergenic
AAATGGGTGTGCTTCCAAAG+AGG	0.650418	1_3:-56621092	Msa0127400:CDS
AACTTCACGGAGTGTGGACC+GGG	0.484362	1_3:+56620867	None:intergenic
AAGAAACTCGTGAACGGTTT+GGG	0.344466	1_3:-56620738	Msa0127400:CDS
AAGCCATTCTATACTTGTGT+TGG	0.563262	1_3:-56620060	Msa0127400:three_prime_UTR
AAGGTTATCTTATCATCAAT+TGG	0.379933	1_3:+56620103	None:intergenic
AATCTAATTTCCAAAATTTG+TGG	0.411855	1_3:+56619956	None:intergenic
AATCTCCGCCGTCTCGTTTC+CGG	0.313382	1_3:-56620946	Msa0127400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGTTAAAGAAACTCGTGAA+CGG	-	chr1_3:56620426-56620445	Msa0127400:CDS	30.0%
TCGATTAGAATGATTTCAGA+AGG	+	chr1_3:56620532-56620551	None:intergenic	30.0%
TCCAACTAACGATTTAAATC+TGG	-	chr1_3:56620651-56620670	Msa0127400:intron	30.0%
ACCAGATTTAAATCGTTAGT+TGG	+	chr1_3:56620655-56620674	None:intergenic	30.0%
ATATGATTGCTGATCAGAAA+TGG	-	chr1_3:56620866-56620885	Msa0127400:CDS	30.0%
AAAAAACCATCAAGATACCA+AGG	+	chr1_3:56620931-56620950	None:intergenic	30.0%
GATAACCTTTGATTGATTGT+AGG	-	chr1_3:56621081-56621100	Msa0127400:CDS	30.0%
GTAAACCTACAATCAATCAA+AGG	+	chr1_3:56621089-56621108	None:intergenic	30.0%
ATACCAACACAAGTATAGAA+TGG	+	chr1_3:56621116-56621135	None:intergenic	30.0%
TTGTTAAAGAAACTCGTGAA+CGG	-	chr1_3:56620426-56620445	Msa0127400:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	56619845	56621347	56619845	ID=Msa0127400;Name=Msa0127400
chr1_3	mRNA	56619845	56621347	56619845	ID=Msa0127400-mRNA-1;Parent=Msa0127400;Name=Msa0127400-mRNA-1;_AED=0.00;_eAED=0.00;_QI=235\|1\|1\|1\|1\|1\|2\|306\|292
chr1_3	exon	56619845	56620593	56619845	ID=Msa0127400-mRNA-1:exon:17951;Parent=Msa0127400-mRNA-1
chr1_3	exon	56620677	56621347	56620677	ID=Msa0127400-mRNA-1:exon:17950;Parent=Msa0127400-mRNA-1
chr1_3	five_prime_UTR	56621113	56621347	56621113	ID=Msa0127400-mRNA-1:five_prime_utr;Parent=Msa0127400-mRNA-1
chr1_3	CDS	56620677	56621112	56620677	ID=Msa0127400-mRNA-1:cds;Parent=Msa0127400-mRNA-1
chr1_3	CDS	56620151	56620593	56620151	ID=Msa0127400-mRNA-1:cds;Parent=Msa0127400-mRNA-1
chr1_3	three_prime_UTR	56619845	56620150	56619845	ID=Msa0127400-mRNA-1:three_prime_utr;Parent=Msa0127400-mRNA-1

Gene Sequence

>Msa0127400

ATGGGTGTGCTTCCAAAGAGGATAATCCTAATGCGTCACGGCGAGTCACAAGGAAACCTTGACACGTCAGCATACACCACCACACCAGACCACAGCATTCAGTTAACTCCATCCGGCATCGCACAAGCCCGCCACGCCGGCGCCAATCTCCGCCGTCTCGTTTCCGGCCAAGGCTGCTCCCCTGACTGGCGTCTCTACTTCTACGTCTCTCCCTACGCCCGCACCCGGTCCACACTCCGTGAAGTTGGTAGATCTTTCTCCAAGAAGCGCGTGATCGGCGTCAGAGAAGAATCGCGCGTACGTGAACAGGATTTCGGTAACTTCCAAGTTCAAGAGCGTATGAAGATTGTTAAAGAAACTCGTGAACGGTTTGGGAGATTCTTTTATCGCTTTCCTGAAGGTGAATCCGCCGCCGATGTTTTCGATCGCATTTCTGGTTTTTTTGAATCTTTATGGAGAGACATTGATTTGAATCGTCTTCATCACGATCCAACTAACGATTTAAATCTGGTGATAGTATCACACGGATTAACATCACGAATTTTCTTGATGAAGTGGTTCAAGTGGACGGTGGAGCAATTTGAGCATCTAAACAATTTCGGAAACTGCGAGTTTCGGGTGATGGAGCAAGGGAGTGGTGGAGAATACAGTTTGGCGATTAATCATACGGAAGAAGAGATGCTTGAATGGGGAATGTCTCCGGATATGATTGCTGATCAGAAATGGCGTGCAGATGCGCCTAGAGGTGCTTGGAATGATCAATGTCCTTGGTATCTTGATGGTTTTTTTGATCGTTTTGCTGAAGATGAATCTGACGATGATGAAGCAATCCAAAATGAAGGTGAAACTGAAATGATAGTTGTTGAAACTAATAAGTAA

Protein sequence

>Msa0127400

MGVLPKRIILMRHGESQGNLDTSAYTTTPDHSIQLTPSGIAQARHAGANLRRLVSGQGCSPDWRLYFYVSPYARTRSTLREVGRSFSKKRVIGVREESRVREQDFGNFQVQERMKIVKETRERFGRFFYRFPEGESAADVFDRISGFFESLWRDIDLNRLHHDPTNDLNLVIVSHGLTSRIFLMKWFKWTVEQFEHLNNFGNCEFRVMEQGSGGEYSLAINHTEEEMLEWGMSPDMIADQKWRADAPRGAWNDQCPWYLDGFFDRFAEDESDDDEAIQNEGETEMIVVETNK*