AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0033140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033140 MtrunA17_Chr1g0191411 97.270 293 6 2 1 292 1 292 0.0 586
Msa0033140 MtrunA17_Chr7g0273211 70.877 285 80 1 2 286 34 315 3.16e-156 438
Msa0033140 MtrunA17_Chr5g0397331 52.060 267 124 3 5 269 82 346 1.09e-93 281
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0033140 AT3G05170.1 72.222 270 75 0 3 272 7 276 3.20e-150 423
Msa0033140 AT1G08940.1 68.953 277 80 3 3 275 7 281 4.35e-142 401

Find 106 sgRNAs with CRISPR-Local

Find 243 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGTTTCACCTTCATTT+TGG 0.169991 1_1:+60438824 None:intergenic
TTTCGGAAACTGCGAGTTTC+GGG 0.182047 1_1:-60439058 Msa0033140:CDS
ACTAATAAGTAACTAATTAA+AGG 0.229674 1_1:-60438787 Msa0033140:exon
CGCATTAGGATTATCCTCTT+TGG 0.271933 1_1:+60439725 None:intergenic
GACGCCGATCACGCGCTTCT+TGG 0.272814 1_1:+60439478 None:intergenic
CTAATTAAAGGGGAATTTAT+AGG 0.274139 1_1:-60438775 Msa0033140:three_prime_UTR
TTTGAGCATCTAAACAATTT+CGG 0.293790 1_1:-60439075 Msa0033140:CDS
TAGAATACAGTTTGGATGTA+TGG 0.300442 1_1:+60439897 None:intergenic
AATCTCCGCCGTCTCGTTTC+CGG 0.313382 1_1:-60439593 Msa0033140:CDS
CTTGAAATTAGAATTCAAAA+TGG 0.319343 1_1:-60439756 Msa0033140:exon
ATTTCGGAAACTGCGAGTTT+CGG 0.331893 1_1:-60439059 Msa0033140:CDS
CGCCGTCTCGTTTCCGGCCA+AGG 0.340582 1_1:-60439587 Msa0033140:CDS
GAGATTGGCGCCGGCGTGGC+GGG 0.342893 1_1:+60439610 None:intergenic
AAGAAACTCGTGAACGGTTT+GGG 0.344466 1_1:-60439385 Msa0033140:CDS
GTGACTCGCCGTGACGCATT+AGG 0.355053 1_1:+60439711 None:intergenic
AGAATCGCGCGTACGTGAAC+AGG 0.355351 1_1:-60439450 Msa0033140:CDS
GATAACCTTTGATTGATTGT+AGG 0.356988 1_1:-60438736 Msa0033140:three_prime_UTR
CTAATAAGTAACTAATTAAA+GGG 0.365006 1_1:-60438786 Msa0033140:exon
TGTGGACCGGGTGCGGGCGT+AGG 0.374827 1_1:+60439526 None:intergenic
AAGGTTATCTTATCATCAAT+TGG 0.379933 1_1:+60438750 None:intergenic
TCGGCGGCGGATTCACCTTC+AGG 0.382270 1_1:+60439344 None:intergenic
CAATGTCCTTGGTATCTTGA+TGG 0.384985 1_1:-60438895 Msa0033140:CDS
AAAGAAACTCGTGAACGGTT+TGG 0.387033 1_1:-60439386 Msa0033140:CDS
GCCAGTCAGGGGAGCAGCCT+TGG 0.397485 1_1:+60439570 None:intergenic
ACCAGATTTAAATCGTTAGT+TGG 0.398319 1_1:+60439165 None:intergenic
GCCAAGGCTGCTCCCCTGAC+TGG 0.403767 1_1:-60439571 Msa0033140:CDS
ACTGTTTGTAGAATACAGTT+TGG 0.406791 1_1:+60439889 None:intergenic
CACGGAGTGTGGACCGGGTG+CGG 0.410340 1_1:+60439519 None:intergenic
AATCTAATTTCCAAAATTTG+TGG 0.411855 1_1:+60438602 None:intergenic
TATGTAATAATCATAATTTA+CGG 0.419568 1_1:-60438672 Msa0033140:three_prime_UTR
CGGAAGAAGAGATGCTTGAA+TGG 0.421203 1_1:-60438987 Msa0033140:CDS
TAGAAGTAGAGACGCCAGTC+AGG 0.422292 1_1:+60439557 None:intergenic
CTAAACGATCCGATTGAGAT+TGG 0.432151 1_1:+60438557 None:intergenic
GTGGACCGGGTGCGGGCGTA+GGG 0.432905 1_1:+60439527 None:intergenic
TCTTCATACGCTCTTGAACT+TGG 0.436566 1_1:+60439414 None:intergenic
GTCAGGGGAGCAGCCTTGGC+CGG 0.437420 1_1:+60439574 None:intergenic
TCCAACTAACGATTTAAATC+TGG 0.438023 1_1:-60439166 Msa0033140:CDS
CGCGTACGTGAACAGGATTT+CGG 0.438914 1_1:-60439443 Msa0033140:CDS
GGAAACGAGACGGCGGAGAT+TGG 0.440959 1_1:+60439595 None:intergenic
GGAAGAAGAGATGCTTGAAT+GGG 0.451438 1_1:-60438986 Msa0033140:CDS
ACGGAGTGTGGACCGGGTGC+GGG 0.458435 1_1:+60439520 None:intergenic
GCTTGAATGGGGAATGTCTC+CGG 0.465250 1_1:-60438974 Msa0033140:CDS
ATATGATTGCTGATCAGAAA+TGG 0.466739 1_1:-60438951 Msa0033140:CDS
GGAGATTGGCGCCGGCGTGG+CGG 0.473230 1_1:+60439609 None:intergenic
TTGAAATTAGAATTCAAAAT+GGG 0.477033 1_1:-60439755 Msa0033140:exon
TTCTGATCAGCAATCATATC+CGG 0.479044 1_1:+60438955 None:intergenic
AACTTCACGGAGTGTGGACC+GGG 0.484362 1_1:+60439514 None:intergenic
GTTAACTGAATGCTGTGGTC+TGG 0.485756 1_1:+60439653 None:intergenic
AAACTGCGAGTTTCGGGTGA+TGG 0.492323 1_1:-60439052 Msa0033140:CDS
GTGGCGGGCTTGTGCGATGC+CGG 0.494843 1_1:+60439625 None:intergenic
AGCATTCAGTTAACTCCATC+CGG 0.508794 1_1:-60439644 Msa0033140:CDS
AGAATACAGTTTGGATGTAT+GGG 0.510305 1_1:+60439898 None:intergenic
CGGGTGATGGAGCAAGGGAG+TGG 0.510953 1_1:-60439039 Msa0033140:CDS
CAGTTTGGCGATTAATCATA+CGG 0.511425 1_1:-60439007 Msa0033140:CDS
ATCGCACAAGCCCGCCACGC+CGG 0.513724 1_1:-60439620 Msa0033140:CDS
TTCTCCAAGAAGCGCGTGAT+CGG 0.514170 1_1:-60439482 Msa0033140:CDS
TCTCTCCCTACGCCCGCACC+CGG 0.515505 1_1:-60439532 Msa0033140:CDS
CAACTTCACGGAGTGTGGAC+CGG 0.516011 1_1:+60439513 None:intergenic
CGGGCTTGTGCGATGCCGGA+TGG 0.517406 1_1:+60439629 None:intergenic
GGCGGAGATTGGCGCCGGCG+TGG 0.518194 1_1:+60439606 None:intergenic
CCAATTTCGATCAGATTACT+CGG 0.523280 1_1:+60438483 None:intergenic
TGTGACCGTCCAATCTCAAT+CGG 0.525925 1_1:-60438566 Msa0033140:three_prime_UTR
GATTACTCGGATCTCAAACT+TGG 0.527329 1_1:+60438496 None:intergenic
AAAACCTATAAATCGAGTAA+AGG 0.531486 1_1:-60439857 Msa0033140:five_prime_UTR
GATGAAGCAATCCAAAATGA+AGG 0.532371 1_1:-60438835 Msa0033140:CDS
AATGCTGTGGTCTGGTGTGG+TGG 0.541324 1_1:+60439661 None:intergenic
GAGTTTCGGGTGATGGAGCA+AGG 0.542115 1_1:-60439045 Msa0033140:CDS
GAGTGGTGGAGAATACAGTT+TGG 0.544566 1_1:-60439022 Msa0033140:CDS
AGAAGTAGAGACGCCAGTCA+GGG 0.558329 1_1:+60439558 None:intergenic
GTGATGGAGCAAGGGAGTGG+TGG 0.558690 1_1:-60439036 Msa0033140:CDS
AAGCCATTCTATACTTGTGT+TGG 0.563262 1_1:-60438707 Msa0033140:three_prime_UTR
TCTACCAACTTCACGGAGTG+TGG 0.563838 1_1:+60439508 None:intergenic
GAGACGGCGGAGATTGGCGC+CGG 0.564282 1_1:+60439601 None:intergenic
GATTGGACGGTCACAATGCA+TGG 0.564493 1_1:+60438574 None:intergenic
CTGAATGCTGTGGTCTGGTG+TGG 0.565165 1_1:+60439658 None:intergenic
TGATCATTCCAAGCACCTCT+AGG 0.566827 1_1:+60438916 None:intergenic
ATACCAACACAAGTATAGAA+TGG 0.569770 1_1:+60438704 None:intergenic
CAGATGCGCCTAGAGGTGCT+TGG 0.570632 1_1:-60438924 Msa0033140:CDS
CTTGGAATGATCAATGTCCT+TGG 0.578748 1_1:-60438906 Msa0033140:CDS
TCTTGATGAAGTGGTTCAAG+TGG 0.585341 1_1:-60439110 Msa0033140:CDS
CGGTCCACACTCCGTGAAGT+TGG 0.587452 1_1:-60439512 Msa0033140:CDS
TGGTGTATGCTGACGTGTCA+AGG 0.588203 1_1:+60439681 None:intergenic
ATGCGTCACGGCGAGTCACA+AGG 0.594731 1_1:-60439707 Msa0033140:CDS
AGAAAGATCTACCAACTTCA+CGG 0.598186 1_1:+60439501 None:intergenic
AGCCTTGGCCGGAAACGAGA+CGG 0.598321 1_1:+60439585 None:intergenic
GTAAACCTACAATCAATCAA+AGG 0.599275 1_1:+60438731 None:intergenic
GAAGTAGAGACGCCAGTCAG+GGG 0.613104 1_1:+60439559 None:intergenic
GCGATCGAAAACATCGGCGG+CGG 0.622403 1_1:+60439331 None:intergenic
TTGTTAAAGAAACTCGTGAA+CGG 0.625373 1_1:-60439391 Msa0033140:CDS
ACGATCCGATTGAGATTGGA+CGG 0.629679 1_1:+60438561 None:intergenic
ACACATTACAGCTGCCGCCG+CGG 0.637118 1_1:-60439951 Msa0033140:five_prime_UTR
AAATGGGTGTGCTTCCAAAG+AGG 0.650418 1_1:-60439739 Msa0033140:CDS
AGTTTCGGGTGATGGAGCAA+GGG 0.652564 1_1:-60439044 Msa0033140:CDS
AATTGATTCTGGTCAAGCCG+CGG 0.657101 1_1:+60439934 None:intergenic
TAATAAGTAACTAATTAAAG+GGG 0.657691 1_1:-60438785 Msa0033140:exon
TGGCGTGCAGATGCGCCTAG+AGG 0.658589 1_1:-60438931 Msa0033140:CDS
GAAGAAGAGATGCTTGAATG+GGG 0.659893 1_1:-60438985 Msa0033140:CDS
TGATTCTGGTCAAGCCGCGG+CGG 0.664767 1_1:+60439937 None:intergenic
AGGATAATCCTAATGCGTCA+CGG 0.674661 1_1:-60439719 Msa0033140:CDS
CTTGGCCGGAAACGAGACGG+CGG 0.676638 1_1:+60439588 None:intergenic
GATGAAGTGGTTCAAGTGGA+CGG 0.691694 1_1:-60439106 Msa0033140:CDS
AGAAATGCGATCGAAAACAT+CGG 0.698994 1_1:+60439325 None:intergenic
ATGGAGTTAACTGAATGCTG+TGG 0.708629 1_1:+60439648 None:intergenic
AATCTGGTGATAGTATCACA+CGG 0.716063 1_1:-60439150 Msa0033140:CDS
GAAGTGGTTCAAGTGGACGG+TGG 0.719720 1_1:-60439103 Msa0033140:CDS
AATGCGATCGAAAACATCGG+CGG 0.731619 1_1:+60439328 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTAATAATCATAATTTA+CGG - chr1_1:60439781-60439800 Msa0033140:five_prime_UTR 10.0%
!! TATGTAATAATCATAATTTA+CGG - chr1_1:60439781-60439800 Msa0033140:five_prime_UTR 10.0%
!! TTGAAATTAGAATTCAAAAT+GGG - chr1_1:60438698-60438717 Msa0033140:three_prime_UTR 15.0%
!!! ATTTGAATTTCTTCTTATTT+AGG - chr1_1:60439213-60439232 Msa0033140:CDS 15.0%
!! ACTAATAAGTAACTAATTAA+AGG - chr1_1:60439666-60439685 Msa0033140:CDS 15.0%
!! CTAATAAGTAACTAATTAAA+GGG - chr1_1:60439667-60439686 Msa0033140:CDS 15.0%
!! TAATAAGTAACTAATTAAAG+GGG - chr1_1:60439668-60439687 Msa0033140:CDS 15.0%
!!! TTTGATTTTCTTCAAAAATT+AGG - chr1_1:60439932-60439951 Msa0033140:five_prime_UTR 15.0%
!! TTGAAATTAGAATTCAAAAT+GGG - chr1_1:60438698-60438717 Msa0033140:three_prime_UTR 15.0%
!!! ATTTGAATTTCTTCTTATTT+AGG - chr1_1:60439213-60439232 Msa0033140:CDS 15.0%
!! ACTAATAAGTAACTAATTAA+AGG - chr1_1:60439666-60439685 Msa0033140:CDS 15.0%
!! CTAATAAGTAACTAATTAAA+GGG - chr1_1:60439667-60439686 Msa0033140:CDS 15.0%
!! TAATAAGTAACTAATTAAAG+GGG - chr1_1:60439668-60439687 Msa0033140:CDS 15.0%
!!! TTTGATTTTCTTCAAAAATT+AGG - chr1_1:60439932-60439951 Msa0033140:five_prime_UTR 15.0%
!! CTTGAAATTAGAATTCAAAA+TGG - chr1_1:60438697-60438716 Msa0033140:three_prime_UTR 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG - chr1_1:60439232-60439251 Msa0033140:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG + chr1_1:60439854-60439873 None:intergenic 20.0%
!! CTTGAAATTAGAATTCAAAA+TGG - chr1_1:60438697-60438716 Msa0033140:three_prime_UTR 20.0%
!!! TAGGTTTTTTTGAATCTTTA+TGG - chr1_1:60439232-60439251 Msa0033140:intron 20.0%
!! AATCTAATTTCCAAAATTTG+TGG + chr1_1:60439854-60439873 None:intergenic 20.0%
!!! AAGATTTTGAGAATTGATTC+TGG + chr1_1:60438533-60438552 None:intergenic 25.0%
! AAAACCTATAAATCGAGTAA+AGG - chr1_1:60438596-60438615 Msa0033140:three_prime_UTR 25.0%
!! TTTTCCTTTACTCGATTTAT+AGG + chr1_1:60438603-60438622 None:intergenic 25.0%
!! AAAAAGAAAGTGTGTTAATC+AGG - chr1_1:60438670-60438689 Msa0033140:three_prime_UTR 25.0%
! TTTGAGCATCTAAACAATTT+CGG - chr1_1:60439378-60439397 Msa0033140:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG - chr1_1:60439678-60439697 Msa0033140:CDS 25.0%
! AAGGTTATCTTATCATCAAT+TGG + chr1_1:60439706-60439725 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG - chr1_1:60439841-60439860 Msa0033140:five_prime_UTR 25.0%
!!! AAGATTTTGAGAATTGATTC+TGG + chr1_1:60438533-60438552 None:intergenic 25.0%
! AAAACCTATAAATCGAGTAA+AGG - chr1_1:60438596-60438615 Msa0033140:three_prime_UTR 25.0%
!! TTTTCCTTTACTCGATTTAT+AGG + chr1_1:60438603-60438622 None:intergenic 25.0%
!! AAAAAGAAAGTGTGTTAATC+AGG - chr1_1:60438670-60438689 Msa0033140:three_prime_UTR 25.0%
! TTTGAGCATCTAAACAATTT+CGG - chr1_1:60439378-60439397 Msa0033140:CDS 25.0%
! CTAATTAAAGGGGAATTTAT+AGG - chr1_1:60439678-60439697 Msa0033140:CDS 25.0%
! AAGGTTATCTTATCATCAAT+TGG + chr1_1:60439706-60439725 None:intergenic 25.0%
!! GATAACTTTTCCACAAATTT+TGG - chr1_1:60439841-60439860 Msa0033140:five_prime_UTR 25.0%
AGAATACAGTTTGGATGTAT+GGG + chr1_1:60438558-60438577 None:intergenic 30.0%
TAGAATACAGTTTGGATGTA+TGG + chr1_1:60438559-60438578 None:intergenic 30.0%
ACTGTTTGTAGAATACAGTT+TGG + chr1_1:60438567-60438586 None:intergenic 30.0%
! CTTTCTTTTTCTTGATAGGT+TGG + chr1_1:60438660-60438679 None:intergenic 30.0%
! CACACTTTCTTTTTCTTGAT+AGG + chr1_1:60438664-60438683 None:intergenic 30.0%
TTGTTAAAGAAACTCGTGAA+CGG - chr1_1:60439062-60439081 Msa0033140:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG + chr1_1:60439168-60439187 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG - chr1_1:60439287-60439306 Msa0033140:intron 30.0%
ACCAGATTTAAATCGTTAGT+TGG + chr1_1:60439291-60439310 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG - chr1_1:60439502-60439521 Msa0033140:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG + chr1_1:60439567-60439586 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG + chr1_1:60439632-60439651 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG - chr1_1:60439717-60439736 Msa0033140:CDS 30.0%
GTAAACCTACAATCAATCAA+AGG + chr1_1:60439725-60439744 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG + chr1_1:60439752-60439771 None:intergenic 30.0%
AGAATACAGTTTGGATGTAT+GGG + chr1_1:60438558-60438577 None:intergenic 30.0%
TAGAATACAGTTTGGATGTA+TGG + chr1_1:60438559-60438578 None:intergenic 30.0%
ACTGTTTGTAGAATACAGTT+TGG + chr1_1:60438567-60438586 None:intergenic 30.0%
! CTTTCTTTTTCTTGATAGGT+TGG + chr1_1:60438660-60438679 None:intergenic 30.0%
! CACACTTTCTTTTTCTTGAT+AGG + chr1_1:60438664-60438683 None:intergenic 30.0%
TTGTTAAAGAAACTCGTGAA+CGG - chr1_1:60439062-60439081 Msa0033140:CDS 30.0%
TCGATTAGAATGATTTCAGA+AGG + chr1_1:60439168-60439187 None:intergenic 30.0%
TCCAACTAACGATTTAAATC+TGG - chr1_1:60439287-60439306 Msa0033140:intron 30.0%
ACCAGATTTAAATCGTTAGT+TGG + chr1_1:60439291-60439310 None:intergenic 30.0%
ATATGATTGCTGATCAGAAA+TGG - chr1_1:60439502-60439521 Msa0033140:CDS 30.0%
AAAAAACCATCAAGATACCA+AGG + chr1_1:60439567-60439586 None:intergenic 30.0%
! TTTCAGTTTCACCTTCATTT+TGG + chr1_1:60439632-60439651 None:intergenic 30.0%
GATAACCTTTGATTGATTGT+AGG - chr1_1:60439717-60439736 Msa0033140:CDS 30.0%
GTAAACCTACAATCAATCAA+AGG + chr1_1:60439725-60439744 None:intergenic 30.0%
ATACCAACACAAGTATAGAA+TGG + chr1_1:60439752-60439771 None:intergenic 30.0%
AGAAAGATCTACCAACTTCA+CGG + chr1_1:60438955-60438974 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG - chr1_1:60439094-60439113 Msa0033140:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG + chr1_1:60439131-60439150 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG + chr1_1:60439159-60439178 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG - chr1_1:60439303-60439322 Msa0033140:intron 35.0%
! CACGAATTTTCTTGATGAAG+TGG - chr1_1:60439334-60439353 Msa0033140:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG - chr1_1:60439446-60439465 Msa0033140:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG + chr1_1:60439501-60439520 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG - chr1_1:60439618-60439637 Msa0033140:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG - chr1_1:60439746-60439765 Msa0033140:exon 35.0%
! CGGATCGTTTAGATTTTAAG+CGG - chr1_1:60439907-60439926 Msa0033140:five_prime_UTR 35.0%
AGAAAGATCTACCAACTTCA+CGG + chr1_1:60438955-60438974 None:intergenic 35.0%
! TTCTTTTATCGCTTTCCTGA+AGG - chr1_1:60439094-60439113 Msa0033140:CDS 35.0%
AGAAATGCGATCGAAAACAT+CGG + chr1_1:60439131-60439150 None:intergenic 35.0%
ATGATTTCAGAAGGAGAAAG+CGG + chr1_1:60439159-60439178 None:intergenic 35.0%
AATCTGGTGATAGTATCACA+CGG - chr1_1:60439303-60439322 Msa0033140:intron 35.0%
! CACGAATTTTCTTGATGAAG+TGG - chr1_1:60439334-60439353 Msa0033140:CDS 35.0%
CAGTTTGGCGATTAATCATA+CGG - chr1_1:60439446-60439465 Msa0033140:CDS 35.0%
TTCTGATCAGCAATCATATC+CGG + chr1_1:60439501-60439520 None:intergenic 35.0%
GATGAAGCAATCCAAAATGA+AGG - chr1_1:60439618-60439637 Msa0033140:CDS 35.0%
AAGCCATTCTATACTTGTGT+TGG - chr1_1:60439746-60439765 Msa0033140:exon 35.0%
! CGGATCGTTTAGATTTTAAG+CGG - chr1_1:60439907-60439926 Msa0033140:five_prime_UTR 35.0%
CGCATTAGGATTATCCTCTT+TGG + chr1_1:60438731-60438750 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG - chr1_1:60438734-60438753 Msa0033140:three_prime_UTR 40.0%
ATGGAGTTAACTGAATGCTG+TGG + chr1_1:60438808-60438827 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG - chr1_1:60438809-60438828 Msa0033140:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG + chr1_1:60439042-60439061 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG - chr1_1:60439067-60439086 Msa0033140:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG - chr1_1:60439068-60439087 Msa0033140:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG - chr1_1:60439130-60439149 Msa0033140:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG - chr1_1:60439343-60439362 Msa0033140:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG - chr1_1:60439394-60439413 Msa0033140:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG - chr1_1:60439467-60439486 Msa0033140:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG - chr1_1:60439468-60439487 Msa0033140:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG - chr1_1:60439547-60439566 Msa0033140:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG - chr1_1:60439558-60439577 Msa0033140:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG + chr1_1:60439899-60439918 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG + chr1_1:60439960-60439979 None:intergenic 40.0%
CGCATTAGGATTATCCTCTT+TGG + chr1_1:60438731-60438750 None:intergenic 40.0%
AGGATAATCCTAATGCGTCA+CGG - chr1_1:60438734-60438753 Msa0033140:three_prime_UTR 40.0%
ATGGAGTTAACTGAATGCTG+TGG + chr1_1:60438808-60438827 None:intergenic 40.0%
AGCATTCAGTTAACTCCATC+CGG - chr1_1:60438809-60438828 Msa0033140:CDS 40.0%
TCTTCATACGCTCTTGAACT+TGG + chr1_1:60439042-60439061 None:intergenic 40.0%
AAAGAAACTCGTGAACGGTT+TGG - chr1_1:60439067-60439086 Msa0033140:CDS 40.0%
AAGAAACTCGTGAACGGTTT+GGG - chr1_1:60439068-60439087 Msa0033140:CDS 40.0%
! GATGTTTTCGATCGCATTTC+TGG - chr1_1:60439130-60439149 Msa0033140:CDS 40.0%
TCTTGATGAAGTGGTTCAAG+TGG - chr1_1:60439343-60439362 Msa0033140:CDS 40.0%
ATTTCGGAAACTGCGAGTTT+CGG - chr1_1:60439394-60439413 Msa0033140:CDS 40.0%
GGAAGAAGAGATGCTTGAAT+GGG - chr1_1:60439467-60439486 Msa0033140:CDS 40.0%
GAAGAAGAGATGCTTGAATG+GGG - chr1_1:60439468-60439487 Msa0033140:CDS 40.0%
CTTGGAATGATCAATGTCCT+TGG - chr1_1:60439547-60439566 Msa0033140:CDS 40.0%
CAATGTCCTTGGTATCTTGA+TGG - chr1_1:60439558-60439577 Msa0033140:CDS 40.0%
CTAAACGATCCGATTGAGAT+TGG + chr1_1:60439899-60439918 None:intergenic 40.0%
GATTACTCGGATCTCAAACT+TGG + chr1_1:60439960-60439979 None:intergenic 40.0%
! AATTGATTCTGGTCAAGCCG+CGG + chr1_1:60438522-60438541 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG - chr1_1:60438714-60438733 Msa0033140:three_prime_UTR 45.0%
GTTAACTGAATGCTGTGGTC+TGG + chr1_1:60438803-60438822 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG + chr1_1:60439128-60439147 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG - chr1_1:60439347-60439366 Msa0033140:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG - chr1_1:60439395-60439414 Msa0033140:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG - chr1_1:60439431-60439450 Msa0033140:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG - chr1_1:60439466-60439485 Msa0033140:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG + chr1_1:60439540-60439559 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG - chr1_1:60439887-60439906 Msa0033140:five_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG + chr1_1:60439895-60439914 None:intergenic 45.0%
CTGTAATGTGTAAGCGAGCA+CGG + chr1_1:60438493-60438512 None:intergenic 45.0%
! AATTGATTCTGGTCAAGCCG+CGG + chr1_1:60438522-60438541 None:intergenic 45.0%
AAATGGGTGTGCTTCCAAAG+AGG - chr1_1:60438714-60438733 Msa0033140:three_prime_UTR 45.0%
GTTAACTGAATGCTGTGGTC+TGG + chr1_1:60438803-60438822 None:intergenic 45.0%
AATGCGATCGAAAACATCGG+CGG + chr1_1:60439128-60439147 None:intergenic 45.0%
GATGAAGTGGTTCAAGTGGA+CGG - chr1_1:60439347-60439366 Msa0033140:CDS 45.0%
TTTCGGAAACTGCGAGTTTC+GGG - chr1_1:60439395-60439414 Msa0033140:CDS 45.0%
! GAGTGGTGGAGAATACAGTT+TGG - chr1_1:60439431-60439450 Msa0033140:CDS 45.0%
CGGAAGAAGAGATGCTTGAA+TGG - chr1_1:60439466-60439485 Msa0033140:CDS 45.0%
TGATCATTCCAAGCACCTCT+AGG + chr1_1:60439540-60439559 None:intergenic 45.0%
TGTGACCGTCCAATCTCAAT+CGG - chr1_1:60439887-60439906 Msa0033140:five_prime_UTR 45.0%
ACGATCCGATTGAGATTGGA+CGG + chr1_1:60439895-60439914 None:intergenic 45.0%
TGGTGTATGCTGACGTGTCA+AGG + chr1_1:60438775-60438794 None:intergenic 50.0%
AGAAGTAGAGACGCCAGTCA+GGG + chr1_1:60438898-60438917 None:intergenic 50.0%
TAGAAGTAGAGACGCCAGTC+AGG + chr1_1:60438899-60438918 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG + chr1_1:60438948-60438967 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG - chr1_1:60438971-60438990 Msa0033140:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG - chr1_1:60439010-60439029 Msa0033140:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG - chr1_1:60439401-60439420 Msa0033140:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG - chr1_1:60439409-60439428 Msa0033140:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG - chr1_1:60439479-60439498 Msa0033140:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG + chr1_1:60439882-60439901 None:intergenic 50.0%
TGGTGTATGCTGACGTGTCA+AGG + chr1_1:60438775-60438794 None:intergenic 50.0%
AGAAGTAGAGACGCCAGTCA+GGG + chr1_1:60438898-60438917 None:intergenic 50.0%
TAGAAGTAGAGACGCCAGTC+AGG + chr1_1:60438899-60438918 None:intergenic 50.0%
TCTACCAACTTCACGGAGTG+TGG + chr1_1:60438948-60438967 None:intergenic 50.0%
! TTCTCCAAGAAGCGCGTGAT+CGG - chr1_1:60438971-60438990 Msa0033140:CDS 50.0%
CGCGTACGTGAACAGGATTT+CGG - chr1_1:60439010-60439029 Msa0033140:CDS 50.0%
! AAACTGCGAGTTTCGGGTGA+TGG - chr1_1:60439401-60439420 Msa0033140:CDS 50.0%
!! AGTTTCGGGTGATGGAGCAA+GGG - chr1_1:60439409-60439428 Msa0033140:CDS 50.0%
GCTTGAATGGGGAATGTCTC+CGG - chr1_1:60439479-60439498 Msa0033140:CDS 50.0%
GATTGGACGGTCACAATGCA+TGG + chr1_1:60439882-60439901 None:intergenic 50.0%
AATGCTGTGGTCTGGTGTGG+TGG + chr1_1:60438795-60438814 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG + chr1_1:60438798-60438817 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG - chr1_1:60438860-60438879 Msa0033140:CDS 55.0%
GAAGTAGAGACGCCAGTCAG+GGG + chr1_1:60438897-60438916 None:intergenic 55.0%
AACTTCACGGAGTGTGGACC+GGG + chr1_1:60438942-60438961 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG + chr1_1:60438943-60438962 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG - chr1_1:60439003-60439022 Msa0033140:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG - chr1_1:60439350-60439369 Msa0033140:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG - chr1_1:60439408-60439427 Msa0033140:CDS 55.0%
AATGCTGTGGTCTGGTGTGG+TGG + chr1_1:60438795-60438814 None:intergenic 55.0%
CTGAATGCTGTGGTCTGGTG+TGG + chr1_1:60438798-60438817 None:intergenic 55.0%
AATCTCCGCCGTCTCGTTTC+CGG - chr1_1:60438860-60438879 Msa0033140:CDS 55.0%
GAAGTAGAGACGCCAGTCAG+GGG + chr1_1:60438897-60438916 None:intergenic 55.0%
AACTTCACGGAGTGTGGACC+GGG + chr1_1:60438942-60438961 None:intergenic 55.0%
CAACTTCACGGAGTGTGGAC+CGG + chr1_1:60438943-60438962 None:intergenic 55.0%
AGAATCGCGCGTACGTGAAC+AGG - chr1_1:60439003-60439022 Msa0033140:CDS 55.0%
GAAGTGGTTCAAGTGGACGG+TGG - chr1_1:60439350-60439369 Msa0033140:CDS 55.0%
!! GAGTTTCGGGTGATGGAGCA+AGG - chr1_1:60439408-60439427 Msa0033140:CDS 55.0%
ACACATTACAGCTGCCGCCG+CGG - chr1_1:60438502-60438521 Msa0033140:three_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG + chr1_1:60438519-60438538 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG + chr1_1:60438745-60438764 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG - chr1_1:60438746-60438765 Msa0033140:three_prime_UTR 60.0%
GGAAACGAGACGGCGGAGAT+TGG + chr1_1:60438861-60438880 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG + chr1_1:60438871-60438890 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG - chr1_1:60438941-60438960 Msa0033140:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG + chr1_1:60439125-60439144 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG - chr1_1:60439417-60439436 Msa0033140:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG - chr1_1:60439529-60439548 Msa0033140:CDS 60.0%
ACACATTACAGCTGCCGCCG+CGG - chr1_1:60438502-60438521 Msa0033140:three_prime_UTR 60.0%
TGATTCTGGTCAAGCCGCGG+CGG + chr1_1:60438519-60438538 None:intergenic 60.0%
GTGACTCGCCGTGACGCATT+AGG + chr1_1:60438745-60438764 None:intergenic 60.0%
ATGCGTCACGGCGAGTCACA+AGG - chr1_1:60438746-60438765 Msa0033140:three_prime_UTR 60.0%
GGAAACGAGACGGCGGAGAT+TGG + chr1_1:60438861-60438880 None:intergenic 60.0%
AGCCTTGGCCGGAAACGAGA+CGG + chr1_1:60438871-60438890 None:intergenic 60.0%
CGGTCCACACTCCGTGAAGT+TGG - chr1_1:60438941-60438960 Msa0033140:CDS 60.0%
GCGATCGAAAACATCGGCGG+CGG + chr1_1:60439125-60439144 None:intergenic 60.0%
! GTGATGGAGCAAGGGAGTGG+TGG - chr1_1:60439417-60439436 Msa0033140:CDS 60.0%
!! CAGATGCGCCTAGAGGTGCT+TGG - chr1_1:60439529-60439548 Msa0033140:CDS 60.0%
CTTGGCCGGAAACGAGACGG+CGG + chr1_1:60438868-60438887 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG + chr1_1:60438978-60438997 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG + chr1_1:60439112-60439131 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG - chr1_1:60439414-60439433 Msa0033140:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG - chr1_1:60439522-60439541 Msa0033140:CDS 65.0%
CTTGGCCGGAAACGAGACGG+CGG + chr1_1:60438868-60438887 None:intergenic 65.0%
GACGCCGATCACGCGCTTCT+TGG + chr1_1:60438978-60438997 None:intergenic 65.0%
! TCGGCGGCGGATTCACCTTC+AGG + chr1_1:60439112-60439131 None:intergenic 65.0%
!! CGGGTGATGGAGCAAGGGAG+TGG - chr1_1:60439414-60439433 Msa0033140:CDS 65.0%
TGGCGTGCAGATGCGCCTAG+AGG - chr1_1:60439522-60439541 Msa0033140:CDS 65.0%
CGGGCTTGTGCGATGCCGGA+TGG + chr1_1:60438827-60438846 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG + chr1_1:60438831-60438850 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG - chr1_1:60438833-60438852 Msa0033140:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG + chr1_1:60438855-60438874 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG - chr1_1:60438866-60438885 Msa0033140:CDS 70.0%
GTCAGGGGAGCAGCCTTGGC+CGG + chr1_1:60438882-60438901 None:intergenic 70.0%
GCCAAGGCTGCTCCCCTGAC+TGG - chr1_1:60438882-60438901 Msa0033140:CDS 70.0%
GCCAGTCAGGGGAGCAGCCT+TGG + chr1_1:60438886-60438905 None:intergenic 70.0%
TCTCTCCCTACGCCCGCACC+CGG - chr1_1:60438921-60438940 Msa0033140:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG + chr1_1:60438936-60438955 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG + chr1_1:60438937-60438956 None:intergenic 70.0%
CGGGCTTGTGCGATGCCGGA+TGG + chr1_1:60438827-60438846 None:intergenic 70.0%
!! GTGGCGGGCTTGTGCGATGC+CGG + chr1_1:60438831-60438850 None:intergenic 70.0%
ATCGCACAAGCCCGCCACGC+CGG - chr1_1:60438833-60438852 Msa0033140:CDS 70.0%
GAGACGGCGGAGATTGGCGC+CGG + chr1_1:60438855-60438874 None:intergenic 70.0%
CGCCGTCTCGTTTCCGGCCA+AGG - chr1_1:60438866-60438885 Msa0033140:CDS 70.0%
GTCAGGGGAGCAGCCTTGGC+CGG + chr1_1:60438882-60438901 None:intergenic 70.0%
GCCAAGGCTGCTCCCCTGAC+TGG - chr1_1:60438882-60438901 Msa0033140:CDS 70.0%
GCCAGTCAGGGGAGCAGCCT+TGG + chr1_1:60438886-60438905 None:intergenic 70.0%
TCTCTCCCTACGCCCGCACC+CGG - chr1_1:60438921-60438940 Msa0033140:CDS 70.0%
ACGGAGTGTGGACCGGGTGC+GGG + chr1_1:60438936-60438955 None:intergenic 70.0%
CACGGAGTGTGGACCGGGTG+CGG + chr1_1:60438937-60438956 None:intergenic 70.0%
!! GAGATTGGCGCCGGCGTGGC+GGG + chr1_1:60438846-60438865 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG + chr1_1:60438847-60438866 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG + chr1_1:60438929-60438948 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG + chr1_1:60438930-60438949 None:intergenic 75.0%
!! GAGATTGGCGCCGGCGTGGC+GGG + chr1_1:60438846-60438865 None:intergenic 75.0%
!! GGAGATTGGCGCCGGCGTGG+CGG + chr1_1:60438847-60438866 None:intergenic 75.0%
GTGGACCGGGTGCGGGCGTA+GGG + chr1_1:60438929-60438948 None:intergenic 75.0%
TGTGGACCGGGTGCGGGCGT+AGG + chr1_1:60438930-60438949 None:intergenic 75.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG + chr1_1:60438850-60438869 None:intergenic 80.0%
!!! GGCGGAGATTGGCGCCGGCG+TGG + chr1_1:60438850-60438869 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 60438491 60439984 60438491 ID=Msa0033140;Name=Msa0033140
chr1_1 mRNA 60438491 60439984 60438491 ID=Msa0033140-mRNA-1;Parent=Msa0033140;Name=Msa0033140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=225|1|1|1|1|1|2|307|292
chr1_1 exon 60438491 60439240 60438491 ID=Msa0033140-mRNA-1:exon:18934;Parent=Msa0033140-mRNA-1
chr1_1 exon 60439324 60439984 60439324 ID=Msa0033140-mRNA-1:exon:18933;Parent=Msa0033140-mRNA-1
chr1_1 five_prime_UTR 60439760 60439984 60439760 ID=Msa0033140-mRNA-1:five_prime_utr;Parent=Msa0033140-mRNA-1
chr1_1 CDS 60439324 60439759 60439324 ID=Msa0033140-mRNA-1:cds;Parent=Msa0033140-mRNA-1
chr1_1 CDS 60438798 60439240 60438798 ID=Msa0033140-mRNA-1:cds;Parent=Msa0033140-mRNA-1
chr1_1 three_prime_UTR 60438491 60438797 60438491 ID=Msa0033140-mRNA-1:three_prime_utr;Parent=Msa0033140-mRNA-1
Gene Sequence

>Msa0033140

ATGGGTGTGCTTCCAAAGAGGATAATCCTAATGCGTCACGGCGAGTCACAAGGAAACCTTGACACGTCAGCATACACCACCACACCAGACCACAGCATTCAGTTAACTCCATCCGGCATCGCACAAGCCCGCCACGCCGGCGCCAATCTCCGCCGTCTCGTTTCCGGCCAAGGCTGCTCCCCTGACTGGCGTCTCTACTTCTACGTCTCTCCCTACGCCCGCACCCGGTCCACACTCCGTGAAGTTGGTAGATCTTTCTCCAAGAAGCGCGTGATCGGCGTCAGAGAAGAATCGCGCGTACGTGAACAGGATTTCGGTAACTTCCAAGTTCAAGAGCGTATGAAGATTGTTAAAGAAACTCGTGAACGGTTTGGGAGATTCTTTTATCGCTTTCCTGAAGGTGAATCCGCCGCCGATGTTTTCGATCGCATTTCTGGTTTTTTTGAATCTTTATGGAGAGACATTGATTTGAATCGTCTTCATCACGATCCAACTAACGATTTAAATCTGGTGATAGTATCACACGGATTAACATCACGAATTTTCTTGATGAAGTGGTTCAAGTGGACGGTGGAGCAATTTGAGCATCTAAACAATTTCGGAAACTGCGAGTTTCGGGTGATGGAGCAAGGGAGTGGTGGAGAATACAGTTTGGCGATTAATCATACGGAAGAAGAGATGCTTGAATGGGGAATGTCTCCGGATATGATTGCTGATCAGAAATGGCGTGCAGATGCGCCTAGAGGTGCTTGGAATGATCAATGTCCTTGGTATCTTGATGGTTTTTTTGATCGTTTTGCTGAAGATGAATCTGACGATGATGAAGCAATCCAAAATGAAGGTGAAACTGAAATGATAGTTGTTGAAACTAATAAGTAA
Protein sequence

>Msa0033140

MGVLPKRIILMRHGESQGNLDTSAYTTTPDHSIQLTPSGIAQARHAGANLRRLVSGQGCSPDWRLYFYVSPYARTRSTLREVGRSFSKKRVIGVREESRVREQDFGNFQVQERMKIVKETRERFGRFFYRFPEGESAADVFDRISGFFESLWRDIDLNRLHHDPTNDLNLVIVSHGLTSRIFLMKWFKWTVEQFEHLNNFGNCEFRVMEQGSGGEYSLAINHTEEEMLEWGMSPDMIADQKWRADAPRGAWNDQCPWYLDGFFDRFAEDESDDDEAIQNEGETEMIVVETNK*