AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172640 MtrunA17_Chr1g0191991 98.305 531 5 3 1 528 1 530 0.0 1068
Msa0172640 MtrunA17_Chr7g0268961 53.721 430 163 12 71 480 32 445 4.54e-134 398
Msa0172640 MtrunA17_Chr2g0330171 59.317 322 73 6 49 347 34 320 1.59e-122 367
Msa0172640 MtrunA17_Chr8g0342421 62.791 301 93 6 59 348 60 352 1.96e-119 361
Msa0172640 MtrunA17_Chr1g0185121 55.807 353 101 10 61 387 40 363 1.24e-117 354
Msa0172640 MtrunA17_Chr8g0373541 81.915 188 30 1 41 224 9 196 7.70e-116 352
Msa0172640 MtrunA17_Chr7g0263941 55.821 335 116 5 30 357 3 312 4.65e-113 341
Msa0172640 MtrunA17_Chr4g0040571 51.482 371 102 10 58 350 36 406 8.47e-113 343
Msa0172640 MtrunA17_Chr4g0029521 45.217 460 176 16 59 479 36 458 6.94e-110 336
Msa0172640 MtrunA17_Chr1g0162971 51.190 336 103 6 61 346 41 365 1.62e-106 325
Msa0172640 MtrunA17_Chr1g0181381 80.925 173 32 1 61 232 38 210 3.75e-106 327
Msa0172640 MtrunA17_Chr1g0151351 80.702 171 32 1 52 221 32 202 2.17e-105 323
Msa0172640 MtrunA17_Chr1g0199101 83.333 162 27 0 59 220 45 206 3.74e-105 325
Msa0172640 MtrunA17_Chr1g0163001 74.866 187 45 1 39 223 19 205 1.46e-104 323
Msa0172640 MtrunA17_Chr1g0210821 84.076 157 25 0 64 220 47 203 5.04e-103 321
Msa0172640 MtrunA17_Chr2g0326381 68.116 207 60 4 60 260 31 237 5.71e-101 310
Msa0172640 MtrunA17_Chr3g0141431 56.090 312 108 9 58 350 19 320 3.86e-99 305
Msa0172640 MtrunA17_Chr2g0324991 90.076 131 13 0 72 202 3 133 6.29e-88 267
Msa0172640 MtrunA17_Chr6g0479921 46.043 278 125 6 61 335 29 284 4.71e-74 242
Msa0172640 MtrunA17_Chr8g0375521 64.744 156 53 2 61 214 27 182 5.68e-72 235
Msa0172640 MtrunA17_Chr1g0200421 33.929 168 95 4 73 224 234 401 9.35e-24 104
Msa0172640 MtrunA17_Chr2g0320341 29.126 206 131 4 19 210 174 378 1.60e-23 102
Msa0172640 MtrunA17_Chr3g0123941 33.696 184 98 4 71 230 118 301 4.15e-23 100
Msa0172640 MtrunA17_Chr8g0352011 35.106 188 96 8 57 226 152 331 2.97e-21 95.1
Msa0172640 MtrunA17_Chr8g0382121 35.811 148 84 5 74 210 109 256 6.50e-21 93.6
Msa0172640 MtrunA17_Chr7g0258011 35.404 161 84 7 84 226 183 341 9.84e-21 93.6
Msa0172640 MtrunA17_Chr5g0422571 35.542 166 88 7 72 226 102 259 5.69e-20 90.5
Msa0172640 MtrunA17_Chr1g0173841 34.104 173 88 8 72 226 216 380 4.47e-19 89.4
Msa0172640 MtrunA17_Chr1g0198651 33.520 179 90 8 72 229 103 273 4.81e-19 87.8
Msa0172640 MtrunA17_Chr2g0328921 35.542 166 87 8 58 213 127 282 2.48e-18 85.9
Msa0172640 MtrunA17_Chr4g0053341 33.113 151 91 4 72 213 69 218 6.84e-18 83.2
Msa0172640 MtrunA17_Chr4g0026521 36.429 140 77 6 84 213 125 262 7.87e-18 84.3
Msa0172640 MtrunA17_Chr2g0321291 36.054 147 82 6 77 213 133 277 1.13e-17 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172640 AT3G50700.1 57.729 317 100 5 61 349 34 344 7.07e-117 353
Msa0172640 AT5G44160.1 57.009 321 105 7 56 345 31 349 5.58e-115 348
Msa0172640 AT1G03840.1 88.304 171 19 1 56 225 35 205 4.82e-113 345
Msa0172640 AT1G03840.2 87.135 171 19 2 56 225 35 203 1.70e-109 335
Msa0172640 AT5G44160.2 55.738 305 103 6 71 345 8 310 7.99e-106 323
Msa0172640 AT1G14580.1 79.412 170 35 0 54 223 46 215 1.25e-105 324
Msa0172640 AT1G14580.2 79.412 170 35 0 54 223 46 215 1.25e-105 324
Msa0172640 AT1G55110.3 74.742 194 39 2 41 224 34 227 1.46e-105 324
Msa0172640 AT1G55110.1 74.742 194 39 2 41 224 34 227 1.46e-105 324
Msa0172640 AT1G55110.2 74.742 194 39 2 41 224 34 227 1.46e-105 324
Msa0172640 AT1G14580.3 79.412 170 35 0 54 223 60 229 2.58e-105 324
Msa0172640 AT3G13810.6 85.294 170 22 2 61 229 51 218 9.57e-105 323
Msa0172640 AT3G13810.5 85.294 170 22 2 61 229 65 232 1.27e-104 323
Msa0172640 AT3G13810.4 85.294 170 22 2 61 229 70 237 1.48e-104 323
Msa0172640 AT3G13810.1 85.294 170 22 2 61 229 70 237 1.48e-104 323
Msa0172640 AT4G02670.2 77.473 182 39 2 52 232 47 227 1.73e-104 319
Msa0172640 AT4G02670.1 77.473 182 39 2 52 232 45 225 1.83e-104 319
Msa0172640 AT3G45260.2 83.537 164 26 1 61 223 39 202 2.39e-104 320
Msa0172640 AT3G45260.1 83.537 164 26 1 61 223 39 202 2.39e-104 320
Msa0172640 AT2G02080.1 79.755 163 33 0 58 220 51 213 5.62e-104 322
Msa0172640 AT2G02080.4 79.755 163 33 0 58 220 51 213 5.62e-104 322
Msa0172640 AT2G02080.5 79.755 163 33 0 58 220 51 213 5.62e-104 322
Msa0172640 AT2G02080.3 79.755 163 33 0 58 220 51 213 5.62e-104 322
Msa0172640 AT5G03150.1 67.742 217 52 3 61 261 53 267 3.06e-101 314
Msa0172640 AT5G60470.2 81.098 164 30 1 61 223 44 207 3.20e-101 313
Msa0172640 AT5G60470.3 81.098 164 30 1 61 223 40 203 3.38e-101 313
Msa0172640 AT2G02070.2 80.368 163 32 0 61 223 52 214 7.38e-101 316
Msa0172640 AT2G02070.1 80.368 163 32 0 61 223 52 214 7.38e-101 316
Msa0172640 AT3G13810.3 85.535 159 20 2 72 229 68 224 7.34e-97 303
Msa0172640 AT3G13810.2 85.535 159 20 2 72 229 82 238 9.80e-97 303
Msa0172640 AT5G60470.1 74.566 173 39 2 56 223 26 198 6.74e-95 296
Msa0172640 AT2G02080.6 38.938 452 168 12 85 480 1 400 5.71e-90 283
Msa0172640 AT2G02080.2 38.938 452 168 12 85 480 1 400 5.71e-90 283
Msa0172640 AT1G68130.1 66.026 156 51 2 61 214 41 196 1.44e-73 239
Msa0172640 AT2G01940.1 61.538 169 63 2 50 216 33 201 4.45e-72 236
Msa0172640 AT1G25250.3 63.694 157 54 2 61 214 33 189 1.74e-71 233
Msa0172640 AT2G01940.3 60.588 170 64 3 50 216 33 202 3.72e-68 226
Msa0172640 AT1G25250.1 64.626 147 49 2 71 214 20 166 9.92e-68 223
Msa0172640 AT1G68130.2 65.455 110 36 2 107 214 1 110 1.12e-46 166
Msa0172640 AT2G01940.2 63.393 112 39 2 107 216 1 112 2.11e-45 163
Msa0172640 AT1G25250.2 62.162 111 39 2 107 214 1 111 1.55e-44 159
Msa0172640 AT5G22890.1 31.667 180 106 5 46 210 174 351 3.12e-23 102
Msa0172640 AT1G34370.3 33.951 162 85 4 70 213 14 171 5.71e-23 99.4
Msa0172640 AT1G34370.2 33.544 158 91 3 70 213 224 381 7.34e-23 102
Msa0172640 AT1G34370.1 33.544 158 91 3 70 213 224 381 7.34e-23 102
Msa0172640 AT1G13290.1 34.591 159 84 7 72 218 89 239 1.04e-18 87.4
Msa0172640 AT3G20880.1 33.125 160 91 5 84 228 250 408 1.97e-18 88.2
Msa0172640 AT3G57670.1 32.370 173 91 7 72 226 205 369 1.98e-18 87.8
Msa0172640 AT1G51220.1 33.113 151 85 5 72 213 166 309 4.88e-18 85.9
Msa0172640 AT1G08290.1 35.294 153 80 7 72 213 168 312 6.05e-17 82.8
Msa0172640 AT1G34790.1 32.667 150 89 5 74 213 129 276 7.33e-16 79.0

Find 149 sgRNAs with CRISPR-Local

Find 250 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTTGTACCACATATTT+TGG 0.125409 1_4:-57810336 None:intergenic
AACATTTGAATACACATTTA+TGG 0.140693 1_4:-57813069 None:intergenic
TTCGGTGCAGTTGAAGATTT+TGG 0.237456 1_4:-57810088 None:intergenic
ACATAGCCAAATTAAGAAAA+AGG 0.238010 1_4:-57808956 None:intergenic
TGATATGTTTAATGCTATAT+TGG 0.247245 1_4:+57812478 Msa0172640:CDS
ATCAAATCATTGATATTAAT+TGG 0.250521 1_4:+57812979 Msa0172640:three_prime_UTR
TACTATTATTAACTATTTCT+TGG 0.251790 1_4:-57812850 None:intergenic
CCCATTATTAACCCTCAAAT+TGG 0.254900 1_4:+57811978 Msa0172640:CDS
AACAACATCTTGGAAGAAAA+TGG 0.256543 1_4:+57812173 Msa0172640:CDS
AATGCAAATGGGATAATAAA+TGG 0.269963 1_4:+57812431 Msa0172640:CDS
CCTCATCTCCACACCATAAA+AGG 0.270952 1_4:+57808742 Msa0172640:five_prime_UTR
ACCAATTTGAGGGTTAATAA+TGG 0.277668 1_4:-57811979 None:intergenic
ACATTTGAATACACATTTAT+GGG 0.280310 1_4:-57813068 None:intergenic
AGATTCAAATCCTCCATTCT+TGG 0.287816 1_4:-57812777 None:intergenic
ATGTTGTTGTTGAAGGGTTT+TGG 0.296073 1_4:-57812083 None:intergenic
CCAATTTGAGGGTTAATAAT+GGG 0.297467 1_4:-57811978 None:intergenic
GGGTTAATAATGGGGTTTAA+AGG 0.300726 1_4:-57811969 None:intergenic
TTTGTGGCTCTCATTTCCTT+TGG 0.301928 1_4:-57813229 Msa0172650:three_prime_UTR
TTTGAAAACACCACTTAGTT+TGG 0.303887 1_4:+57812819 Msa0172640:exon
GTGAAGTTGCAGCAGATAAA+TGG 0.308637 1_4:-57812202 None:intergenic
TTGTGGCTCTCATTTCCTTT+GGG 0.313829 1_4:-57813228 Msa0172650:three_prime_UTR
AATAAATTACCTGGATTTCC+TGG 0.317784 1_4:-57809173 None:intergenic
AATAATAATTTCAATGCAAA+TGG 0.327404 1_4:+57812419 Msa0172640:CDS
CTGATGAGGTTTGGAGAAAT+TGG 0.331929 1_4:+57812750 Msa0172640:CDS
TTTGTAGCCATTAGTGTTGT+TGG 0.339715 1_4:-57810023 None:intergenic
ACTCTTGTCTTGAGAAACTA+TGG 0.354103 1_4:-57808867 None:intergenic
CAGCAGCAGCTGATGAGGTT+TGG 0.362969 1_4:+57812741 Msa0172640:CDS
CAAAGCATTAATAGTGTTGT+TGG 0.386850 1_4:+57812548 Msa0172640:CDS
TCCTGTTCAATATTCACGTT+CGG 0.390930 1_4:+57813124 Msa0172640:three_prime_UTR
AAAATACAAGAAACAGGCTT+TGG 0.391584 1_4:-57808703 None:intergenic
GGTGCACATAGTAATTTCTA+TGG 0.395859 1_4:+57812680 Msa0172640:CDS
CTTAATTGAAACAGAAAAGA+TGG 0.398347 1_4:-57808808 None:intergenic
AGATCACCAAGTGCTCTTGC+AGG 0.399590 1_4:-57810209 None:intergenic
GTTGTTGGAGGTGGAGGAGG+AGG 0.400532 1_4:+57812563 Msa0172640:CDS
GGAGGAGGCGTTGGAATGAA+TGG 0.403069 1_4:+57812452 Msa0172640:CDS
AAACACCACTTAGTTTGGTT+GGG 0.406309 1_4:+57812824 Msa0172640:three_prime_UTR
TGTGTATTCAAATGTTATAA+TGG 0.406710 1_4:+57813076 Msa0172640:three_prime_UTR
GCTACAGAAAGCTGCTCAAA+TGG 0.409352 1_4:+57812247 Msa0172640:CDS
TGCTATATTGGATCAAAGTA+AGG 0.411463 1_4:+57812490 Msa0172640:CDS
AATAATGTTATGAACATTAG+TGG 0.411466 1_4:+57812602 Msa0172640:CDS
TGGAGATGAGGGGTGCTTGT+TGG 0.412864 1_4:-57808730 None:intergenic
TATGAACATTAGTGGAAGTA+AGG 0.415131 1_4:+57812610 Msa0172640:CDS
AATAGTGTTGTTGGAGGTGG+AGG 0.417136 1_4:+57812557 Msa0172640:CDS
AATATGCTGTTCAATCTGAT+TGG 0.427517 1_4:+57810303 Msa0172640:CDS
AGTGAATATGTTGTTGTTGA+AGG 0.428537 1_4:-57812090 None:intergenic
AGTGTTGTTGGAGGTGGAGG+AGG 0.433876 1_4:+57812560 Msa0172640:CDS
AGAGCACTTGGTGATCTTAC+TGG 0.434819 1_4:+57810215 Msa0172640:CDS
ATAAAAGGAGGAGAGTTTCC+TGG 0.442673 1_4:+57808757 Msa0172640:five_prime_UTR
AAAACACCACTTAGTTTGGT+TGG 0.442676 1_4:+57812823 Msa0172640:exon
ATGATGGCGCCGCCGCAGTT+TGG 0.444697 1_4:+57812332 Msa0172640:CDS
AACAGAAAAGATGGGATTTG+TGG 0.453016 1_4:-57808799 None:intergenic
CAAGTGCTCTTGCAGGGTTA+TGG 0.453384 1_4:-57810202 None:intergenic
TGGTGAACACATGAAGGTTC+AGG 0.454246 1_4:-57810182 None:intergenic
TCCGAACGTGAATATTGAAC+AGG 0.454569 1_4:-57813125 None:intergenic
AACCACCTCTTAAGAAGAAA+AGG 0.456615 1_4:+57809144 Msa0172640:CDS
CAACAAACTGATCAAATACT+TGG 0.458193 1_4:+57809047 Msa0172640:CDS
AGTTCATGAGGTGATGATAA+TGG 0.461625 1_4:-57812044 None:intergenic
GAGGAAACGAGATTTGAGAT+TGG 0.462517 1_4:-57811942 None:intergenic
ACTATCACCAACAACACTAA+TGG 0.464691 1_4:+57810016 Msa0172640:CDS
AAGGCAAGTTGTGTCCTCTT+CGG 0.471989 1_4:-57810106 None:intergenic
TGTAATGACACTGGATTTGT+TGG 0.477727 1_4:+57812643 Msa0172640:CDS
ATAATAAATGGAGGAGGCGT+TGG 0.478927 1_4:+57812443 Msa0172640:CDS
AAACGAGATTTGAGATTGGT+TGG 0.484142 1_4:-57811938 None:intergenic
AGAAATTGGTCAACCAAGAA+TGG 0.485147 1_4:+57812764 Msa0172640:CDS
GATTTGTTGGGGATAGGAGG+AGG 0.488522 1_4:+57812656 Msa0172640:CDS
TAATTCACTCAACAACATCT+TGG 0.492063 1_4:+57812163 Msa0172640:CDS
AGGAACCTACCAGGAAATCC+AGG 0.493855 1_4:+57809164 Msa0172640:CDS
CTGGATTTGTTGGGGATAGG+AGG 0.495376 1_4:+57812653 Msa0172640:CDS
GATACAGTTCCACCAATTTG+AGG 0.499903 1_4:-57811990 None:intergenic
CATAACCCTGCAAGAGCACT+TGG 0.500225 1_4:+57810203 Msa0172640:CDS
ATACAGTTCCACCAATTTGA+GGG 0.501003 1_4:-57811989 None:intergenic
ATTATTAACCCTCAAATTGG+TGG 0.502270 1_4:+57811981 Msa0172640:CDS
GTGTCATGGTCGAAAGGAAG+GGG 0.506196 1_4:-57812956 None:intergenic
AGTGCTATCACTTCAGCACT+TGG 0.507626 1_4:-57809996 None:intergenic
CTACAGAAAGCTGCTCAAAT+GGG 0.511542 1_4:+57812248 Msa0172640:CDS
AACACCACTTAGTTTGGTTG+GGG 0.514202 1_4:+57812825 Msa0172640:three_prime_UTR
ATTAATAGTGTTGTTGGAGG+TGG 0.514406 1_4:+57812554 Msa0172640:CDS
CGCCATCATGTTTGTAGCAA+CGG 0.514892 1_4:-57812318 None:intergenic
TTTGAACTGAGTTGAAGAGA+AGG 0.515296 1_4:-57812140 None:intergenic
AACATCACAAGTTGTGATAG+TGG 0.515608 1_4:+57808981 Msa0172640:CDS
ATGTGTCATGGTCGAAAGGA+AGG 0.516071 1_4:-57812958 None:intergenic
GTTGTTGCTGTTGCATGTAG+TGG 0.516364 1_4:-57812388 None:intergenic
GTAATGACACTGGATTTGTT+GGG 0.517404 1_4:+57812644 Msa0172640:CDS
GAGATGAAGTTGATGATGAA+AGG 0.518078 1_4:-57812118 None:intergenic
GTGAATATGTTGTTGTTGAA+GGG 0.519299 1_4:-57812089 None:intergenic
AATTACCTGGATTTCCTGGT+AGG 0.520896 1_4:-57809169 None:intergenic
ATTCCAAAATATGTGGTACA+AGG 0.520937 1_4:+57810333 Msa0172640:CDS
TTAATTGAAACAGAAAAGAT+GGG 0.527878 1_4:-57808807 None:intergenic
GAAGAGGACACAACTTGCCT+TGG 0.527889 1_4:+57810108 Msa0172640:CDS
ATAATAATTTCAATGCAAAT+GGG 0.528123 1_4:+57812420 Msa0172640:CDS
ACATACATACCTGGAAAAGA+TGG 0.528499 1_4:-57810378 None:intergenic
AACATTAGTGGAAGTAAGGG+TGG 0.535289 1_4:+57812614 Msa0172640:CDS
CACATAGTAATTTCTATGGA+GGG 0.535940 1_4:+57812684 Msa0172640:CDS
TTTGTTGAAGTATGAGAATG+TGG 0.537355 1_4:-57809083 None:intergenic
ACTGTGAGTAATAATAATGC+AGG 0.538175 1_4:+57812275 Msa0172640:CDS
CAATTTGAGGGTTAATAATG+GGG 0.543880 1_4:-57811977 None:intergenic
CTAGTGCAGAAATTAGAAAG+AGG 0.551088 1_4:+57810147 Msa0172640:CDS
AATGGGGTTTAAAGGTAATG+AGG 0.552566 1_4:-57811961 None:intergenic
ATTAGTGGAAGTAAGGGTGG+TGG 0.553858 1_4:+57812617 Msa0172640:CDS
AGCATTAATAGTGTTGTTGG+AGG 0.564315 1_4:+57812551 Msa0172640:CDS
GCACATAGTAATTTCTATGG+AGG 0.570101 1_4:+57812683 Msa0172640:CDS
TTGTTGGGGATAGGAGGAGG+AGG 0.571045 1_4:+57812659 Msa0172640:CDS
TGCGTCACAGAATGCTCTGT+GGG 0.571848 1_4:-57811862 None:intergenic
AAAGCTCATTCCAAAATATG+TGG 0.572953 1_4:+57810326 Msa0172640:CDS
ACACTGGATTTGTTGGGGAT+AGG 0.573986 1_4:+57812650 Msa0172640:CDS
TAGTGCAGAAATTAGAAAGA+GGG 0.575321 1_4:+57810148 Msa0172640:CDS
TACCGTTGCTACAAACATGA+TGG 0.575806 1_4:+57812316 Msa0172640:CDS
CATAGTAATTTCTATGGAGG+GGG 0.576896 1_4:+57812686 Msa0172640:CDS
CGTATGTGAAATATGCAACA+AGG 0.589045 1_4:+57810052 Msa0172640:CDS
ATGAACATTAGTGGAAGTAA+GGG 0.589532 1_4:+57812611 Msa0172640:CDS
GAACGTGAATATTGAACAGG+AGG 0.590078 1_4:-57813122 None:intergenic
TGTGTCATGGTCGAAAGGAA+GGG 0.590566 1_4:-57812957 None:intergenic
ACATAGTAATTTCTATGGAG+GGG 0.591190 1_4:+57812685 Msa0172640:CDS
GATCACCAAGTGCTCTTGCA+GGG 0.594376 1_4:-57810208 None:intergenic
TGTCATGGTCGAAAGGAAGG+GGG 0.594893 1_4:-57812955 None:intergenic
ACATCACAAGTTGTGATAGT+GGG 0.595444 1_4:+57808982 Msa0172640:CDS
GGGTTATGGTGAACACATGA+AGG 0.595455 1_4:-57810188 None:intergenic
AAGAAGAAAAGGAACCTACC+AGG 0.596110 1_4:+57809155 Msa0172640:CDS
ATCAATGATTTGATGTGTCA+TGG 0.599292 1_4:-57812970 None:intergenic
GGCTCCCCAACCAAACTAAG+TGG 0.601479 1_4:-57812829 None:intergenic
AGCTTTCTGTAGCAATGCAG+TGG 0.610000 1_4:-57812237 None:intergenic
TTCCAAAATATGTGGTACAA+GGG 0.611826 1_4:+57810334 Msa0172640:CDS
ACAAGCTATCTAGGAAGCAG+AGG 0.613878 1_4:-57808834 None:intergenic
ATGGAAGCTTGTGATCTGAA+TGG 0.617097 1_4:-57812025 None:intergenic
AATCTTCAACTGCACCGAAG+AGG 0.621618 1_4:+57810092 Msa0172640:CDS
TTATGTGTAGACTCTTATGA+TGG 0.635701 1_4:+57813044 Msa0172640:three_prime_UTR
TAATGACACTGGATTTGTTG+GGG 0.637157 1_4:+57812645 Msa0172640:CDS
AATTGGTCAACCAAGAATGG+AGG 0.638927 1_4:+57812767 Msa0172640:CDS
GTATGTGAAATATGCAACAA+GGG 0.639897 1_4:+57810053 Msa0172640:CDS
AATGGGATAATAAATGGAGG+AGG 0.644754 1_4:+57812437 Msa0172640:CDS
TGGTGGAGATGTAATGACAC+TGG 0.649106 1_4:+57812634 Msa0172640:CDS
GTCATGGTCGAAAGGAAGGG+GGG 0.655496 1_4:-57812954 None:intergenic
TGCACTACACCAAACTGCGG+CGG 0.655717 1_4:-57812341 None:intergenic
GCAAATGGGATAATAAATGG+AGG 0.656300 1_4:+57812434 Msa0172640:CDS
TCTACACATAACACTTACAC+TGG 0.657671 1_4:-57813032 None:intergenic
GATGGGATTTGTGGAACCAG+AGG 0.657823 1_4:-57808790 None:intergenic
TTTGATGTGTCATGGTCGAA+AGG 0.659881 1_4:-57812962 None:intergenic
TAATAATGCAGGTATCATGA+CGG 0.660595 1_4:+57812286 Msa0172640:CDS
CATCTCCACACCATAAAAGG+AGG 0.660797 1_4:+57808745 Msa0172640:five_prime_UTR
AGGGAATACAAATGTGACTG+TGG 0.667636 1_4:+57810353 Msa0172640:CDS
ATGCGTCACAGAATGCTCTG+TGG 0.669753 1_4:-57811863 None:intergenic
TTTGGTGTAGTGCAACAACA+AGG 0.680990 1_4:+57812350 Msa0172640:CDS
GTTGCAGCAGATAAATGGAG+TGG 0.688832 1_4:-57812197 None:intergenic
CTCTTGACTTGAGAACCCAA+AGG 0.695930 1_4:+57813213 Msa0172640:three_prime_UTR
GAGAACAACAAAGCAAACGA+AGG 0.700937 1_4:+57811894 Msa0172640:CDS
ACTACTGACATGAGTTCATG+AGG 0.716939 1_4:-57812056 None:intergenic
TGTTGCACTACACCAAACTG+CGG 0.718918 1_4:-57812344 None:intergenic
AACGTGAATATTGAACAGGA+GGG 0.719567 1_4:-57813121 None:intergenic
AATGCAGTGGCTGACATGTG+AGG 0.731584 1_4:-57812224 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACTAAATAGAAAAAAA+AGG + chr1_4:57808917-57808936 Msa0172640:five_prime_UTR 10.0%
!! TAAACTAAATAGAAAAAAAA+GGG + chr1_4:57808918-57808937 Msa0172640:five_prime_UTR 10.0%
!! AATAATAATAATAATAGCAA+TGG + chr1_4:57809104-57809123 Msa0172640:CDS 10.0%
!! TAAAATAAATGATGAATTAT+TGG + chr1_4:57809816-57809835 Msa0172640:intron 10.0%
!! TAAAAGAAAAAAGATATAAA+AGG - chr1_4:57810897-57810916 None:intergenic 10.0%
!!! TTTTATTTTTAAAGATAAGT+TGG + chr1_4:57811105-57811124 Msa0172640:intron 10.0%
!! AACATAAATGTAAATTATTT+GGG - chr1_4:57811226-57811245 None:intergenic 10.0%
!! AAACATAAATGTAAATTATT+TGG - chr1_4:57811227-57811246 None:intergenic 10.0%
!! CAATATGCAAATAAAATTAA+AGG - chr1_4:57809258-57809277 None:intergenic 15.0%
!! TGTTACATAATTAAAATCAA+GGG - chr1_4:57809296-57809315 None:intergenic 15.0%
!! TTGTTACATAATTAAAATCA+AGG - chr1_4:57809297-57809316 None:intergenic 15.0%
!!! TACATAATTATTGTTATTCA+TGG + chr1_4:57809384-57809403 Msa0172640:intron 15.0%
!!! ACATAATTATTGTTATTCAT+GGG + chr1_4:57809385-57809404 Msa0172640:intron 15.0%
!!! TATTTTTTTTTACCTCATAT+TGG - chr1_4:57809613-57809632 None:intergenic 15.0%
!! TTTAATTAAGACATTTCTAT+AGG + chr1_4:57809663-57809682 Msa0172640:intron 15.0%
!!! TTTTTAACTTTTTGTAGTTT+AGG + chr1_4:57810610-57810629 Msa0172640:intron 15.0%
!!! TTCTTTAAGAAATTGTATAA+TGG + chr1_4:57810945-57810964 Msa0172640:intron 15.0%
!! CTAATTAATAATAAGAGTTT+AGG + chr1_4:57810998-57811017 Msa0172640:intron 15.0%
!! TAAAGAAAAATACTTACTTA+GGG + chr1_4:57811039-57811058 Msa0172640:intron 15.0%
!!! TCTTCATTGTTTTTTTTTTA+TGG + chr1_4:57811174-57811193 Msa0172640:intron 15.0%
!!! GCAATTTGTTATTTTTTTTA+AGG - chr1_4:57811406-57811425 None:intergenic 15.0%
!! ATATATTATAATTACTCATG+AGG - chr1_4:57811724-57811743 None:intergenic 15.0%
!! AATAATAATTTCAATGCAAA+TGG + chr1_4:57812419-57812438 Msa0172640:CDS 15.0%
!! ATAATAATTTCAATGCAAAT+GGG + chr1_4:57812420-57812439 Msa0172640:CDS 15.0%
!!! TACTATTATTAACTATTTCT+TGG - chr1_4:57812853-57812872 None:intergenic 15.0%
!! ATCAAATCATTGATATTAAT+TGG + chr1_4:57812979-57812998 Msa0172640:three_prime_UTR 15.0%
!! TTAATTGAAACAGAAAAGAT+GGG - chr1_4:57808810-57808829 None:intergenic 20.0%
!! AGAATACAAAATAAATTACC+TGG - chr1_4:57809185-57809204 None:intergenic 20.0%
!!! TTTTTGTGAGATATCATTTT+TGG - chr1_4:57809588-57809607 None:intergenic 20.0%
!! AAAAATGATATCTCACAAAA+AGG + chr1_4:57809587-57809606 Msa0172640:intron 20.0%
!! AGGTAAAAAAAAATAGACAA+TGG + chr1_4:57809618-57809637 Msa0172640:intron 20.0%
!! CTTCACTTAAAAGAATAATT+AGG - chr1_4:57809738-57809757 None:intergenic 20.0%
!!! AATAACGTGTTTAATTTGTT+TGG + chr1_4:57809966-57809985 Msa0172640:intron 20.0%
!! GTAAAGAAAAATACTTACTT+AGG + chr1_4:57811038-57811057 Msa0172640:intron 20.0%
!! TGATATGTTTAATGCTATAT+TGG + chr1_4:57812478-57812497 Msa0172640:CDS 20.0%
!! AATAATGTTATGAACATTAG+TGG + chr1_4:57812602-57812621 Msa0172640:CDS 20.0%
!!! TATTCAACTTTTTGAATTAG+TGG + chr1_4:57812871-57812890 Msa0172640:three_prime_UTR 20.0%
!!! ATTCAACTTTTTGAATTAGT+GGG + chr1_4:57812872-57812891 Msa0172640:three_prime_UTR 20.0%
!! ACATTTGAATACACATTTAT+GGG - chr1_4:57813071-57813090 None:intergenic 20.0%
!! AACATTTGAATACACATTTA+TGG - chr1_4:57813072-57813091 None:intergenic 20.0%
!! TGTGTATTCAAATGTTATAA+TGG + chr1_4:57813076-57813095 Msa0172640:three_prime_UTR 20.0%
!!! ATTTTAATTTGTGCATTCAT+TGG - chr1_4:57813181-57813200 None:intergenic 20.0%
! CTTAATTGAAACAGAAAAGA+TGG - chr1_4:57808811-57808830 None:intergenic 25.0%
! AAGAAAAAAACAAGCTATCT+AGG - chr1_4:57808846-57808865 None:intergenic 25.0%
!! CTACTTCCTTTTTCTTAATT+TGG + chr1_4:57808950-57808969 Msa0172640:five_prime_UTR 25.0%
! ACATAGCCAAATTAAGAAAA+AGG - chr1_4:57808959-57808978 None:intergenic 25.0%
! TTGTTATAGAATATGATGCT+TGG - chr1_4:57809431-57809450 None:intergenic 25.0%
!! TTTAGAATTAACCAATGAGA+AGG - chr1_4:57809506-57809525 None:intergenic 25.0%
!! AGTCTTATCCTTTAATTTCA+TGG - chr1_4:57809691-57809710 None:intergenic 25.0%
! AAGACAAAGATCTATATAGT+GGG + chr1_4:57809755-57809774 Msa0172640:intron 25.0%
! GTGTGAAACAAAGAAAAAAT+AGG - chr1_4:57809927-57809946 None:intergenic 25.0%
! GAAATGTGAATAAAAACTTG+TGG + chr1_4:57810403-57810422 Msa0172640:intron 25.0%
!!! TTATTTTCCTTTTTGAGCAA+TGG + chr1_4:57810692-57810711 Msa0172640:intron 25.0%
!!! TCACAAAAGTTGTTGATTTT+TGG - chr1_4:57810729-57810748 None:intergenic 25.0%
!!! TCTACTTTTCCTTTGAAAAT+TGG - chr1_4:57810758-57810777 None:intergenic 25.0%
! AAACACAGATGTAAAATGTT+TGG + chr1_4:57811064-57811083 Msa0172640:intron 25.0%
!! ATATGAATGATGATGATTTG+CGG - chr1_4:57811290-57811309 None:intergenic 25.0%
! GTAACTTATAATTGTACTCA+AGG - chr1_4:57811592-57811611 None:intergenic 25.0%
! ATTAATAAGAAACACTAGCT+AGG - chr1_4:57811803-57811822 None:intergenic 25.0%
! AATGCAAATGGGATAATAAA+TGG + chr1_4:57812431-57812450 Msa0172640:CDS 25.0%
!! TTCAACTTTTTGAATTAGTG+GGG + chr1_4:57812873-57812892 Msa0172640:three_prime_UTR 25.0%
AAAATACAAGAAACAGGCTT+TGG - chr1_4:57808706-57808725 None:intergenic 30.0%
GGCATAAAAATACAAGAAAC+AGG - chr1_4:57808712-57808731 None:intergenic 30.0%
CAACAAACTGATCAAATACT+TGG + chr1_4:57809047-57809066 Msa0172640:CDS 30.0%
TTTGTTGAAGTATGAGAATG+TGG - chr1_4:57809086-57809105 None:intergenic 30.0%
AATAAATTACCTGGATTTCC+TGG - chr1_4:57809176-57809195 None:intergenic 30.0%
TAGAATATGATGCTTGGAAT+TGG - chr1_4:57809425-57809444 None:intergenic 30.0%
!! TTTTGGAAACGAAGATCATT+TGG - chr1_4:57809571-57809590 None:intergenic 30.0%
TATAGGCTCCATGAAATTAA+AGG + chr1_4:57809680-57809699 Msa0172640:intron 30.0%
TTAAAGGATAAGACTTGATC+TGG + chr1_4:57809696-57809715 Msa0172640:intron 30.0%
GAAGACAAAGATCTATATAG+TGG + chr1_4:57809754-57809773 Msa0172640:intron 30.0%
AGACAAAGATCTATATAGTG+GGG + chr1_4:57809756-57809775 Msa0172640:intron 30.0%
CAACAGTGTACAACAATTAT+TGG - chr1_4:57809891-57809910 None:intergenic 30.0%
GTATGTGAAATATGCAACAA+GGG + chr1_4:57810053-57810072 Msa0172640:CDS 30.0%
TAGTGCAGAAATTAGAAAGA+GGG + chr1_4:57810148-57810167 Msa0172640:CDS 30.0%
AATATGCTGTTCAATCTGAT+TGG + chr1_4:57810303-57810322 Msa0172640:CDS 30.0%
AAAGCTCATTCCAAAATATG+TGG + chr1_4:57810326-57810345 Msa0172640:CDS 30.0%
ATTCCAAAATATGTGGTACA+AGG + chr1_4:57810333-57810352 Msa0172640:CDS 30.0%
TTCCAAAATATGTGGTACAA+GGG + chr1_4:57810334-57810353 Msa0172640:CDS 30.0%
CGCACACACTTTATTATTAT+TGG - chr1_4:57810506-57810525 None:intergenic 30.0%
AGCTAAGAATAAGATAGACT+GGG + chr1_4:57810538-57810557 Msa0172640:intron 30.0%
!! GAAAAGTGTTCTTAGTTCAT+AGG - chr1_4:57810577-57810596 None:intergenic 30.0%
TAGAATACCATTGCTCAAAA+AGG - chr1_4:57810702-57810721 None:intergenic 30.0%
!! ACTTTTCCTTTGAAAATTGG+TGG - chr1_4:57810755-57810774 None:intergenic 30.0%
ACTTTCTCTTTCTATTGCTT+TGG - chr1_4:57810795-57810814 None:intergenic 30.0%
GTATATTATAATACCCATGC+AGG + chr1_4:57811697-57811716 Msa0172640:intron 30.0%
CAATTTGAGGGTTAATAATG+GGG - chr1_4:57811980-57811999 None:intergenic 30.0%
CCAATTTGAGGGTTAATAAT+GGG - chr1_4:57811981-57812000 None:intergenic 30.0%
ACCAATTTGAGGGTTAATAA+TGG - chr1_4:57811982-57812001 None:intergenic 30.0%
ATTATTAACCCTCAAATTGG+TGG + chr1_4:57811981-57812000 Msa0172640:CDS 30.0%
! GTGAATATGTTGTTGTTGAA+GGG - chr1_4:57812092-57812111 None:intergenic 30.0%
! AGTGAATATGTTGTTGTTGA+AGG - chr1_4:57812093-57812112 None:intergenic 30.0%
TAATTCACTCAACAACATCT+TGG + chr1_4:57812163-57812182 Msa0172640:CDS 30.0%
AACAACATCTTGGAAGAAAA+TGG + chr1_4:57812173-57812192 Msa0172640:CDS 30.0%
ACTGTGAGTAATAATAATGC+AGG + chr1_4:57812275-57812294 Msa0172640:CDS 30.0%
TAATAATGCAGGTATCATGA+CGG + chr1_4:57812286-57812305 Msa0172640:CDS 30.0%
TGCTATATTGGATCAAAGTA+AGG + chr1_4:57812490-57812509 Msa0172640:CDS 30.0%
! CAAAGCATTAATAGTGTTGT+TGG + chr1_4:57812548-57812567 Msa0172640:CDS 30.0%
TATGAACATTAGTGGAAGTA+AGG + chr1_4:57812610-57812629 Msa0172640:CDS 30.0%
ATGAACATTAGTGGAAGTAA+GGG + chr1_4:57812611-57812630 Msa0172640:CDS 30.0%
CACATAGTAATTTCTATGGA+GGG + chr1_4:57812684-57812703 Msa0172640:CDS 30.0%
ACATAGTAATTTCTATGGAG+GGG + chr1_4:57812685-57812704 Msa0172640:CDS 30.0%
TTTGAAAACACCACTTAGTT+TGG + chr1_4:57812819-57812838 Msa0172640:exon 30.0%
ATCAATGATTTGATGTGTCA+TGG - chr1_4:57812973-57812992 None:intergenic 30.0%
TTATGTGTAGACTCTTATGA+TGG + chr1_4:57813044-57813063 Msa0172640:three_prime_UTR 30.0%
AACAGAAAAGATGGGATTTG+TGG - chr1_4:57808802-57808821 None:intergenic 35.0%
ACTCTTGTCTTGAGAAACTA+TGG - chr1_4:57808870-57808889 None:intergenic 35.0%
AACATCACAAGTTGTGATAG+TGG + chr1_4:57808981-57809000 Msa0172640:CDS 35.0%
ACATCACAAGTTGTGATAGT+GGG + chr1_4:57808982-57809001 Msa0172640:CDS 35.0%
AACCACCTCTTAAGAAGAAA+AGG + chr1_4:57809144-57809163 Msa0172640:CDS 35.0%
! TTCCTTTTCTTCTTAAGAGG+TGG - chr1_4:57809149-57809168 None:intergenic 35.0%
! AGGTTCCTTTTCTTCTTAAG+AGG - chr1_4:57809152-57809171 None:intergenic 35.0%
ACTTCTTTCAACCTTCTCAT+TGG + chr1_4:57809492-57809511 Msa0172640:intron 35.0%
ACTATCACCAACAACACTAA+TGG + chr1_4:57810016-57810035 Msa0172640:CDS 35.0%
TTTGTAGCCATTAGTGTTGT+TGG - chr1_4:57810026-57810045 None:intergenic 35.0%
CGTATGTGAAATATGCAACA+AGG + chr1_4:57810052-57810071 Msa0172640:CDS 35.0%
!!! GTTCTTTGTTTTAGCTTCCA+AGG - chr1_4:57810128-57810147 None:intergenic 35.0%
CTAGTGCAGAAATTAGAAAG+AGG + chr1_4:57810147-57810166 Msa0172640:CDS 35.0%
! AAGCACTTTTGTAGAAAGCA+TGG + chr1_4:57810245-57810264 Msa0172640:CDS 35.0%
! TTCCCTTGTACCACATATTT+TGG - chr1_4:57810339-57810358 None:intergenic 35.0%
ACATACATACCTGGAAAAGA+TGG - chr1_4:57810381-57810400 None:intergenic 35.0%
ACATTTCACACATACATACC+TGG - chr1_4:57810390-57810409 None:intergenic 35.0%
CTTATTCTTAGCTGCCAAAA+CGG - chr1_4:57810531-57810550 None:intergenic 35.0%
CAGCTAAGAATAAGATAGAC+TGG + chr1_4:57810537-57810556 Msa0172640:intron 35.0%
! TGACAACCACCAATTTTCAA+AGG + chr1_4:57810746-57810765 Msa0172640:intron 35.0%
GAGAGATAAAGTTGTCACAT+AGG + chr1_4:57810834-57810853 Msa0172640:intron 35.0%
TCACATAGGTTGTCACAAAA+TGG + chr1_4:57810848-57810867 Msa0172640:intron 35.0%
TCAAAGATTCCTACGTATGT+AGG + chr1_4:57811648-57811667 Msa0172640:intron 35.0%
CAAAGATTCCTACGTATGTA+GGG + chr1_4:57811649-57811668 Msa0172640:intron 35.0%
TTTATACACCCTACATACGT+AGG - chr1_4:57811660-57811679 None:intergenic 35.0%
! AAACGAGATTTGAGATTGGT+TGG - chr1_4:57811941-57811960 None:intergenic 35.0%
AATGGGGTTTAAAGGTAATG+AGG - chr1_4:57811964-57811983 None:intergenic 35.0%
! GGGTTAATAATGGGGTTTAA+AGG - chr1_4:57811972-57811991 None:intergenic 35.0%
CCCATTATTAACCCTCAAAT+TGG + chr1_4:57811978-57811997 Msa0172640:CDS 35.0%
ATACAGTTCCACCAATTTGA+GGG - chr1_4:57811992-57812011 None:intergenic 35.0%
AGTTCATGAGGTGATGATAA+TGG - chr1_4:57812047-57812066 None:intergenic 35.0%
!! ATGTTGTTGTTGAAGGGTTT+TGG - chr1_4:57812086-57812105 None:intergenic 35.0%
!! GAGATGAAGTTGATGATGAA+AGG - chr1_4:57812121-57812140 None:intergenic 35.0%
! TTTGAACTGAGTTGAAGAGA+AGG - chr1_4:57812143-57812162 None:intergenic 35.0%
GCAAATGGGATAATAAATGG+AGG + chr1_4:57812434-57812453 Msa0172640:CDS 35.0%
AATGGGATAATAAATGGAGG+AGG + chr1_4:57812437-57812456 Msa0172640:CDS 35.0%
! AGCATTAATAGTGTTGTTGG+AGG + chr1_4:57812551-57812570 Msa0172640:CDS 35.0%
! ATTAATAGTGTTGTTGGAGG+TGG + chr1_4:57812554-57812573 Msa0172640:CDS 35.0%
TGTAATGACACTGGATTTGT+TGG + chr1_4:57812643-57812662 Msa0172640:CDS 35.0%
! GTAATGACACTGGATTTGTT+GGG + chr1_4:57812644-57812663 Msa0172640:CDS 35.0%
!! TAATGACACTGGATTTGTTG+GGG + chr1_4:57812645-57812664 Msa0172640:CDS 35.0%
GGTGCACATAGTAATTTCTA+TGG + chr1_4:57812680-57812699 Msa0172640:CDS 35.0%
GCACATAGTAATTTCTATGG+AGG + chr1_4:57812683-57812702 Msa0172640:CDS 35.0%
CATAGTAATTTCTATGGAGG+GGG + chr1_4:57812686-57812705 Msa0172640:CDS 35.0%
AGAAATTGGTCAACCAAGAA+TGG + chr1_4:57812764-57812783 Msa0172640:CDS 35.0%
AGATTCAAATCCTCCATTCT+TGG - chr1_4:57812780-57812799 None:intergenic 35.0%
! AAAACACCACTTAGTTTGGT+TGG + chr1_4:57812823-57812842 Msa0172640:exon 35.0%
! AAACACCACTTAGTTTGGTT+GGG + chr1_4:57812824-57812843 Msa0172640:three_prime_UTR 35.0%
TCTACACATAACACTTACAC+TGG - chr1_4:57813035-57813054 None:intergenic 35.0%
AACGTGAATATTGAACAGGA+GGG - chr1_4:57813124-57813143 None:intergenic 35.0%
TCCTGTTCAATATTCACGTT+CGG + chr1_4:57813124-57813143 Msa0172640:three_prime_UTR 35.0%
AGAGCCACAAAAACTAGATA+AGG + chr1_4:57813241-57813260 Msa0172640:three_prime_UTR 35.0%
! TTTTATGGTGTGGAGATGAG+GGG - chr1_4:57808743-57808762 None:intergenic 40.0%
! CTTTTATGGTGTGGAGATGA+GGG - chr1_4:57808744-57808763 None:intergenic 40.0%
! AGGAAACTCTCCTCCTTTTA+TGG - chr1_4:57808758-57808777 None:intergenic 40.0%
ATAAAAGGAGGAGAGTTTCC+TGG + chr1_4:57808757-57808776 Msa0172640:five_prime_UTR 40.0%
AAGAAGAAAAGGAACCTACC+AGG + chr1_4:57809155-57809174 Msa0172640:CDS 40.0%
AATTACCTGGATTTCCTGGT+AGG - chr1_4:57809172-57809191 None:intergenic 40.0%
CTCACAAAAAGGCCAATATG+AGG + chr1_4:57809598-57809617 Msa0172640:intron 40.0%
GTGTGTGTAAGTAAGTAGCT+AGG + chr1_4:57809838-57809857 Msa0172640:intron 40.0%
!! TTCGGTGCAGTTGAAGATTT+TGG - chr1_4:57810091-57810110 None:intergenic 40.0%
! GAAAGCATGGTGAGAAAAAG+TGG + chr1_4:57810258-57810277 Msa0172640:CDS 40.0%
AGGGAATACAAATGTGACTG+TGG + chr1_4:57810353-57810372 Msa0172640:CDS 40.0%
GTTCTTAGTTCATAGGTGTG+TGG - chr1_4:57810570-57810589 None:intergenic 40.0%
CGGTGTAGAGCATATTAAAG+TGG - chr1_4:57811270-57811289 None:intergenic 40.0%
GAGAACAACAAAGCAAACGA+AGG + chr1_4:57811894-57811913 Msa0172640:CDS 40.0%
! GAGGAAACGAGATTTGAGAT+TGG - chr1_4:57811945-57811964 None:intergenic 40.0%
GATACAGTTCCACCAATTTG+AGG - chr1_4:57811993-57812012 None:intergenic 40.0%
ATGGAAGCTTGTGATCTGAA+TGG - chr1_4:57812028-57812047 None:intergenic 40.0%
ACTACTGACATGAGTTCATG+AGG - chr1_4:57812059-57812078 None:intergenic 40.0%
!! TTGTTGTTGAAGGGTTTTGG+TGG - chr1_4:57812083-57812102 None:intergenic 40.0%
GTGAAGTTGCAGCAGATAAA+TGG - chr1_4:57812205-57812224 None:intergenic 40.0%
CTACAGAAAGCTGCTCAAAT+GGG + chr1_4:57812248-57812267 Msa0172640:CDS 40.0%
TACCGTTGCTACAAACATGA+TGG + chr1_4:57812316-57812335 Msa0172640:CDS 40.0%
! TTTGGTGTAGTGCAACAACA+AGG + chr1_4:57812350-57812369 Msa0172640:CDS 40.0%
! ATAATAAATGGAGGAGGCGT+TGG + chr1_4:57812443-57812462 Msa0172640:CDS 40.0%
AACATTAGTGGAAGTAAGGG+TGG + chr1_4:57812614-57812633 Msa0172640:CDS 40.0%
CTGATGAGGTTTGGAGAAAT+TGG + chr1_4:57812750-57812769 Msa0172640:CDS 40.0%
AATTGGTCAACCAAGAATGG+AGG + chr1_4:57812767-57812786 Msa0172640:CDS 40.0%
! AACACCACTTAGTTTGGTTG+GGG + chr1_4:57812825-57812844 Msa0172640:three_prime_UTR 40.0%
TTTGATGTGTCATGGTCGAA+AGG - chr1_4:57812965-57812984 None:intergenic 40.0%
GAACGTGAATATTGAACAGG+AGG - chr1_4:57813125-57813144 None:intergenic 40.0%
TCCGAACGTGAATATTGAAC+AGG - chr1_4:57813128-57813147 None:intergenic 40.0%
TTGTGGCTCTCATTTCCTTT+GGG - chr1_4:57813231-57813250 Msa0172650:three_prime_UTR 40.0%
TTTGTGGCTCTCATTTCCTT+TGG - chr1_4:57813232-57813251 Msa0172650:three_prime_UTR 40.0%
! CCTTTTATGGTGTGGAGATG+AGG - chr1_4:57808745-57808764 None:intergenic 45.0%
CCTCATCTCCACACCATAAA+AGG + chr1_4:57808742-57808761 Msa0172640:five_prime_UTR 45.0%
CATCTCCACACCATAAAAGG+AGG + chr1_4:57808745-57808764 Msa0172640:five_prime_UTR 45.0%
! ACTCTCCTCCTTTTATGGTG+TGG - chr1_4:57808753-57808772 None:intergenic 45.0%
!! TCCTGGTTTTTGTCTTCCTC+TGG + chr1_4:57808774-57808793 Msa0172640:five_prime_UTR 45.0%
ACCAGAGGAAGACAAAAACC+AGG - chr1_4:57808778-57808797 None:intergenic 45.0%
ACAAGCTATCTAGGAAGCAG+AGG - chr1_4:57808837-57808856 None:intergenic 45.0%
AGTGCTATCACTTCAGCACT+TGG - chr1_4:57809999-57810018 None:intergenic 45.0%
AATCTTCAACTGCACCGAAG+AGG + chr1_4:57810092-57810111 Msa0172640:CDS 45.0%
AAGGCAAGTTGTGTCCTCTT+CGG - chr1_4:57810109-57810128 None:intergenic 45.0%
TGGTGAACACATGAAGGTTC+AGG - chr1_4:57810185-57810204 None:intergenic 45.0%
GGGTTATGGTGAACACATGA+AGG - chr1_4:57810191-57810210 None:intergenic 45.0%
! AGAGCACTTGGTGATCTTAC+TGG + chr1_4:57810215-57810234 Msa0172640:CDS 45.0%
! ACTGTGGAACCATCTTTTCC+AGG + chr1_4:57810369-57810388 Msa0172640:CDS 45.0%
TTACTCATGAGGACCTGCAT+GGG - chr1_4:57811713-57811732 None:intergenic 45.0%
ATTACTCATGAGGACCTGCA+TGG - chr1_4:57811714-57811733 None:intergenic 45.0%
GTTGCAGCAGATAAATGGAG+TGG - chr1_4:57812200-57812219 None:intergenic 45.0%
AGCTTTCTGTAGCAATGCAG+TGG - chr1_4:57812240-57812259 None:intergenic 45.0%
GCTACAGAAAGCTGCTCAAA+TGG + chr1_4:57812247-57812266 Msa0172640:CDS 45.0%
CGCCATCATGTTTGTAGCAA+CGG - chr1_4:57812321-57812340 None:intergenic 45.0%
TGTTGCACTACACCAAACTG+CGG - chr1_4:57812347-57812366 None:intergenic 45.0%
! GTTGTTGCTGTTGCATGTAG+TGG - chr1_4:57812391-57812410 None:intergenic 45.0%
! AATAGTGTTGTTGGAGGTGG+AGG + chr1_4:57812557-57812576 Msa0172640:CDS 45.0%
ATTAGTGGAAGTAAGGGTGG+TGG + chr1_4:57812617-57812636 Msa0172640:CDS 45.0%
TGGTGGAGATGTAATGACAC+TGG + chr1_4:57812634-57812653 Msa0172640:CDS 45.0%
!! ACACTGGATTTGTTGGGGAT+AGG + chr1_4:57812650-57812669 Msa0172640:CDS 45.0%
TGTGTCATGGTCGAAAGGAA+GGG - chr1_4:57812960-57812979 None:intergenic 45.0%
ATGTGTCATGGTCGAAAGGA+AGG - chr1_4:57812961-57812980 None:intergenic 45.0%
! CTCTTGACTTGAGAACCCAA+AGG + chr1_4:57813213-57813232 Msa0172640:three_prime_UTR 45.0%
!! TATATAGTAATAAATTTATT+TGG + chr1_4:57811620-57811639 Msa0172640:intron 5.0%
GATGGGATTTGTGGAACCAG+AGG - chr1_4:57808793-57808812 None:intergenic 50.0%
AGGAACCTACCAGGAAATCC+AGG + chr1_4:57809164-57809183 Msa0172640:CDS 50.0%
GAAGAGGACACAACTTGCCT+TGG + chr1_4:57810108-57810127 Msa0172640:CDS 50.0%
CAAGTGCTCTTGCAGGGTTA+TGG - chr1_4:57810205-57810224 None:intergenic 50.0%
!! CATAACCCTGCAAGAGCACT+TGG + chr1_4:57810203-57810222 Msa0172640:CDS 50.0%
GATCACCAAGTGCTCTTGCA+GGG - chr1_4:57810211-57810230 None:intergenic 50.0%
AGATCACCAAGTGCTCTTGC+AGG - chr1_4:57810212-57810231 None:intergenic 50.0%
!!! AAAGTGTGTGCGTGCCGTTT+TGG + chr1_4:57810514-57810533 Msa0172640:intron 50.0%
TGCGTCACAGAATGCTCTGT+GGG - chr1_4:57811865-57811884 None:intergenic 50.0%
ATGCGTCACAGAATGCTCTG+TGG - chr1_4:57811866-57811885 None:intergenic 50.0%
AATGCAGTGGCTGACATGTG+AGG - chr1_4:57812227-57812246 None:intergenic 50.0%
!! CTGGATTTGTTGGGGATAGG+AGG + chr1_4:57812653-57812672 Msa0172640:CDS 50.0%
!! GATTTGTTGGGGATAGGAGG+AGG + chr1_4:57812656-57812675 Msa0172640:CDS 50.0%
! AGGGGGTACACAACAACAAC+AGG + chr1_4:57812703-57812722 Msa0172640:CDS 50.0%
TGTCATGGTCGAAAGGAAGG+GGG - chr1_4:57812958-57812977 None:intergenic 50.0%
GTGTCATGGTCGAAAGGAAG+GGG - chr1_4:57812959-57812978 None:intergenic 50.0%
!! TGGAGATGAGGGGTGCTTGT+TGG - chr1_4:57808733-57808752 None:intergenic 55.0%
TGCACTACACCAAACTGCGG+CGG - chr1_4:57812344-57812363 None:intergenic 55.0%
!! GGAGGAGGCGTTGGAATGAA+TGG + chr1_4:57812452-57812471 Msa0172640:CDS 55.0%
! AGTGTTGTTGGAGGTGGAGG+AGG + chr1_4:57812560-57812579 Msa0172640:CDS 55.0%
TTGTTGGGGATAGGAGGAGG+AGG + chr1_4:57812659-57812678 Msa0172640:CDS 55.0%
CAGCAGCAGCTGATGAGGTT+TGG + chr1_4:57812741-57812760 Msa0172640:CDS 55.0%
GGCTCCCCAACCAAACTAAG+TGG - chr1_4:57812832-57812851 None:intergenic 55.0%
GTCATGGTCGAAAGGAAGGG+GGG - chr1_4:57812957-57812976 None:intergenic 55.0%
GTTGTTGGAGGTGGAGGAGG+AGG + chr1_4:57812563-57812582 Msa0172640:CDS 60.0%
AGCAGCAGCAGCAGCTGATG+AGG + chr1_4:57812736-57812755 Msa0172640:CDS 60.0%
! TCGAAAGGAAGGGGGGTGTG+GGG - chr1_4:57812950-57812969 None:intergenic 60.0%
! GTCGAAAGGAAGGGGGGTGT+GGG - chr1_4:57812951-57812970 None:intergenic 60.0%
! ATGATGGCGCCGCCGCAGTT+TGG + chr1_4:57812332-57812351 Msa0172640:CDS 65.0%
GGTCGAAAGGAAGGGGGGTG+TGG - chr1_4:57812952-57812971 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 57808685 57813264 57808685 ID=Msa0172640;Name=Msa0172640
chr1_4 mRNA 57808685 57813264 57808685 ID=Msa0172640-mRNA-1;Parent=Msa0172640;Name=Msa0172640-mRNA-1;_AED=0.27;_eAED=0.27;_QI=290|1|1|1|1|1|3|441|528
chr1_4 exon 57808685 57809185 57808685 ID=Msa0172640-mRNA-1:exon:17722;Parent=Msa0172640-mRNA-1
chr1_4 exon 57809994 57810390 57809994 ID=Msa0172640-mRNA-1:exon:17723;Parent=Msa0172640-mRNA-1
chr1_4 exon 57811845 57813264 57811845 ID=Msa0172640-mRNA-1:exon:17724;Parent=Msa0172640-mRNA-1
chr1_4 five_prime_UTR 57808685 57808974 57808685 ID=Msa0172640-mRNA-1:five_prime_utr;Parent=Msa0172640-mRNA-1
chr1_4 CDS 57808975 57809185 57808975 ID=Msa0172640-mRNA-1:cds;Parent=Msa0172640-mRNA-1
chr1_4 CDS 57809994 57810390 57809994 ID=Msa0172640-mRNA-1:cds;Parent=Msa0172640-mRNA-1
chr1_4 CDS 57811845 57812823 57811845 ID=Msa0172640-mRNA-1:cds;Parent=Msa0172640-mRNA-1
chr1_4 three_prime_UTR 57812824 57813264 57812824 ID=Msa0172640-mRNA-1:three_prime_utr;Parent=Msa0172640-mRNA-1
Gene Sequence

>Msa0172640

ATGTCAAACATCACAAGTTGTGATAGTGGGAGTTTCTCTACAGAGAATACAAGAGAAGATGCAGTTAAACAGCAACAAACTGATCAAATACTTGGTCAATTTCATAGTCCACATTCTCATACTTCAACAAATAATAATAATAATAGCAATGGTTCTAACACAGATTTACAACCACCTCTTAAGAAGAAAAGGAACCTACCAGGAAATCCAGATCCAAGTGCTGAAGTGATAGCACTATCACCAACAACACTAATGGCTACAAACAGATTCGTATGTGAAATATGCAACAAGGGTTTTCAAAGAGACCAAAATCTTCAACTGCACCGAAGAGGACACAACTTGCCTTGGAAGCTAAAACAAAGAACTAGTGCAGAAATTAGAAAGAGGGTTTATGTTTGTCCTGAACCTTCATGTGTTCACCATAACCCTGCAAGAGCACTTGGTGATCTTACTGGTATCAAAAAGCACTTTTGTAGAAAGCATGGTGAGAAAAAGTGGAAATGTGATAAGTGTTCTAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCATTCCAAAATATGTGGTACAAGGGAATACAAATGTGACTGTGGAACCATCTTTTCCAGAAGAGACAGCTTCATTACCCACAGAGCATTCTGTGACGCATTAGCTGAAGAGAACAACAAAGCAAACGAAGGAGTACTATCAAATTTGCAACACCAACCAATCTCAAATCTCGTTTCCTCATTACCTTTAAACCCCATTATTAACCCTCAAATTGGTGGAACTGTATCAGAATTCAACAACCATTCAGATCACAAGCTTCCATTATCATCACCTCATGAACTCATGTCAGTAGTGCCACCAAAACCCTTCAACAACAACATATTCACTAGAAGCCTTTCATCATCAACTTCATCTCCTTCTCTTCAACTCAGTTCAAATAATTCACTCAACAACATCTTGGAAGAAAATGGACCACTCCATTTATCTGCTGCAACTTCACCTCACATGTCAGCCACTGCATTGCTACAGAAAGCTGCTCAAATGGGTGCAACTGTGAGTAATAATAATGCAGGTATCATGACGGATAAAACTACCGTTGCTACAAACATGATGGCGCCGCCGCAGTTTGGTGTAGTGCAACAACAAGGACATGCTTTCATGAACCACTACATGCAACAGCAACAACAACACTACAATAATAATTTCAATGCAAATGGGATAATAAATGGAGGAGGCGTTGGAATGAATGGAGTTGATATGTTTAATGCTATATTGGATCAAAGTAAGGCTTTATCGAAGATCATTGAACAAAACAATCAAACTCAAAGCATTAATAGTGTTGTTGGAGGTGGAGGAGGAGGTGCATCAAGTAATAGTAATAATGTTATGAACATTAGTGGAAGTAAGGGTGGTGGAGATGTAATGACACTGGATTTGTTGGGGATAGGAGGAGGAGGTGCACATAGTAATTTCTATGGAGGGGGTACACAACAACAACAGGCAGAAAGTGCAGCAGCAGCAGCAGCTGATGAGGTTTGGAGAAATTGGTCAACCAAGAATGGAGGATTTGAATCTTTTTCAGCAACAAGCAACATTTGA
Protein sequence

>Msa0172640

MSNITSCDSGSFSTENTREDAVKQQQTDQILGQFHSPHSHTSTNNNNNSNGSNTDLQPPLKKKRNLPGNPDPSAEVIALSPTTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRTSAEIRKRVYVCPEPSCVHHNPARALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKICGTREYKCDCGTIFSRRDSFITHRAFCDALAEENNKANEGVLSNLQHQPISNLVSSLPLNPIINPQIGGTVSEFNNHSDHKLPLSSPHELMSVVPPKPFNNNIFTRSLSSSTSSPSLQLSSNNSLNNILEENGPLHLSAATSPHMSATALLQKAAQMGATVSNNNAGIMTDKTTVATNMMAPPQFGVVQQQGHAFMNHYMQQQQQHYNNNFNANGIINGGGVGMNGVDMFNAILDQSKALSKIIEQNNQTQSINSVVGGGGGGASSNSNNVMNISGSKGGGDVMTLDLLGIGGGGAHSNFYGGGTQQQQAESAAAAAADEVWRNWSTKNGGFESFSATSNI*