AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1405050

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1405050 MtrunA17_Chr1g0191991 98.493 531 4 3 1 528 1 530 0.0 1070
Msa1405050 MtrunA17_Chr7g0268961 53.721 430 163 12 71 480 32 445 1.87e-134 399
Msa1405050 MtrunA17_Chr2g0330171 60.841 309 89 5 49 347 34 320 2.80e-122 367
Msa1405050 MtrunA17_Chr8g0342421 61.238 307 88 6 59 348 60 352 2.37e-118 358
Msa1405050 MtrunA17_Chr1g0185121 57.225 346 107 11 61 387 40 363 1.11e-117 354
Msa1405050 MtrunA17_Chr8g0373541 81.915 188 30 1 41 224 9 196 4.74e-116 352
Msa1405050 MtrunA17_Chr4g0040571 51.752 371 101 10 58 350 36 406 1.07e-113 346
Msa1405050 MtrunA17_Chr7g0263941 55.821 335 116 5 30 357 3 312 4.31e-113 341
Msa1405050 MtrunA17_Chr4g0029521 44.396 455 187 15 59 479 36 458 7.99e-109 333
Msa1405050 MtrunA17_Chr1g0162971 51.190 336 103 6 61 346 41 365 9.33e-107 326
Msa1405050 MtrunA17_Chr1g0181381 80.925 173 32 1 61 232 38 210 2.60e-106 327
Msa1405050 MtrunA17_Chr1g0151351 80.702 171 32 1 52 221 32 202 2.05e-105 323
Msa1405050 MtrunA17_Chr1g0199101 83.333 162 27 0 59 220 45 206 2.19e-105 326
Msa1405050 MtrunA17_Chr1g0163001 74.866 187 45 1 39 223 19 205 1.09e-104 323
Msa1405050 MtrunA17_Chr1g0210821 84.076 157 25 0 64 220 47 203 4.07e-103 321
Msa1405050 MtrunA17_Chr2g0326381 68.116 207 60 4 60 260 31 237 5.12e-101 310
Msa1405050 MtrunA17_Chr3g0141431 78.882 161 33 1 58 217 19 179 5.50e-97 300
Msa1405050 MtrunA17_Chr2g0324991 90.076 131 13 0 72 202 3 133 6.56e-88 267
Msa1405050 MtrunA17_Chr6g0479921 46.043 278 125 6 61 335 29 284 1.90e-74 243
Msa1405050 MtrunA17_Chr8g0375521 64.744 156 53 2 61 214 27 182 4.18e-72 236
Msa1405050 MtrunA17_Chr1g0200421 33.929 168 95 4 73 224 234 401 9.18e-24 104
Msa1405050 MtrunA17_Chr2g0320341 29.126 206 131 4 19 210 174 378 1.63e-23 102
Msa1405050 MtrunA17_Chr3g0123941 33.696 184 98 4 71 230 118 301 4.60e-23 100
Msa1405050 MtrunA17_Chr8g0352011 35.106 188 96 8 57 226 152 331 2.89e-21 95.1
Msa1405050 MtrunA17_Chr8g0382121 35.811 148 84 5 74 210 109 256 6.38e-21 93.6
Msa1405050 MtrunA17_Chr7g0258011 35.404 161 84 7 84 226 183 341 9.48e-21 93.6
Msa1405050 MtrunA17_Chr5g0422571 35.542 166 88 7 72 226 102 259 4.94e-20 90.5
Msa1405050 MtrunA17_Chr1g0173841 34.104 173 88 8 72 226 216 380 4.23e-19 89.4
Msa1405050 MtrunA17_Chr1g0198651 33.520 179 90 8 72 229 103 273 5.70e-19 87.4
Msa1405050 MtrunA17_Chr2g0328921 35.542 166 87 8 58 213 127 282 2.17e-18 86.3
Msa1405050 MtrunA17_Chr4g0053341 33.113 151 91 4 72 213 69 218 6.46e-18 83.2
Msa1405050 MtrunA17_Chr4g0026521 36.429 140 77 6 84 213 125 262 7.30e-18 84.3
Msa1405050 MtrunA17_Chr2g0321291 36.054 147 82 6 77 213 133 277 1.08e-17 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1405050 AT3G50700.1 58.044 317 99 5 61 349 34 344 2.50e-116 351
Msa1405050 AT5G44160.1 56.698 321 106 7 56 345 31 349 8.20e-114 345
Msa1405050 AT1G03840.1 88.304 171 19 1 56 225 35 205 3.13e-113 345
Msa1405050 AT1G03840.2 87.135 171 19 2 56 225 35 203 1.32e-109 336
Msa1405050 AT2G02080.1 41.719 477 174 14 58 480 51 477 4.29e-106 327
Msa1405050 AT2G02080.4 41.719 477 174 14 58 480 51 477 4.29e-106 327
Msa1405050 AT2G02080.5 41.719 477 174 14 58 480 51 477 4.29e-106 327
Msa1405050 AT2G02080.3 41.719 477 174 14 58 480 51 477 4.29e-106 327
Msa1405050 AT1G14580.1 79.412 170 35 0 54 223 46 215 9.90e-106 325
Msa1405050 AT1G14580.2 79.412 170 35 0 54 223 46 215 9.90e-106 325
Msa1405050 AT1G55110.3 74.742 194 39 2 41 224 34 227 1.07e-105 324
Msa1405050 AT1G55110.1 74.742 194 39 2 41 224 34 227 1.07e-105 324
Msa1405050 AT1G55110.2 74.742 194 39 2 41 224 34 227 1.07e-105 324
Msa1405050 AT1G14580.3 79.412 170 35 0 54 223 60 229 1.95e-105 324
Msa1405050 AT3G13810.6 85.294 170 22 2 61 229 51 218 8.50e-105 323
Msa1405050 AT3G13810.5 85.294 170 22 2 61 229 65 232 1.13e-104 323
Msa1405050 AT3G13810.4 85.294 170 22 2 61 229 70 237 1.36e-104 323
Msa1405050 AT3G13810.1 85.294 170 22 2 61 229 70 237 1.36e-104 323
Msa1405050 AT5G44160.2 55.410 305 104 6 71 345 8 310 1.43e-104 320
Msa1405050 AT4G02670.2 77.473 182 39 2 52 232 47 227 1.49e-104 319
Msa1405050 AT4G02670.1 77.473 182 39 2 52 232 45 225 1.54e-104 319
Msa1405050 AT3G45260.2 83.537 164 26 1 61 223 39 202 1.86e-104 321
Msa1405050 AT3G45260.1 83.537 164 26 1 61 223 39 202 1.86e-104 321
Msa1405050 AT5G03150.1 84.472 161 24 1 61 220 53 213 2.49e-101 315
Msa1405050 AT5G60470.2 81.098 164 30 1 61 223 44 207 2.64e-101 313
Msa1405050 AT5G60470.3 81.098 164 30 1 61 223 40 203 2.72e-101 313
Msa1405050 AT2G02070.2 80.368 163 32 0 61 223 52 214 5.96e-101 317
Msa1405050 AT2G02070.1 80.368 163 32 0 61 223 52 214 5.96e-101 317
Msa1405050 AT3G13810.3 85.535 159 20 2 72 229 68 224 6.88e-97 303
Msa1405050 AT3G13810.2 85.535 159 20 2 72 229 82 238 8.08e-97 303
Msa1405050 AT5G60470.1 74.566 173 39 2 56 223 26 198 6.96e-95 296
Msa1405050 AT2G02080.6 39.778 450 167 14 85 480 1 400 2.42e-90 284
Msa1405050 AT2G02080.2 39.778 450 167 14 85 480 1 400 2.42e-90 284
Msa1405050 AT1G68130.1 66.026 156 51 2 61 214 41 196 1.23e-73 240
Msa1405050 AT2G01940.1 61.538 169 63 2 50 216 33 201 3.88e-72 237
Msa1405050 AT1G25250.3 63.694 157 54 2 61 214 33 189 1.47e-71 233
Msa1405050 AT2G01940.3 60.588 170 64 3 50 216 33 202 3.11e-68 226
Msa1405050 AT1G25250.1 64.626 147 49 2 71 214 20 166 8.47e-68 223
Msa1405050 AT1G68130.2 65.455 110 36 2 107 214 1 110 9.21e-47 166
Msa1405050 AT2G01940.2 63.393 112 39 2 107 216 1 112 1.76e-45 164
Msa1405050 AT1G25250.2 62.162 111 39 2 107 214 1 111 1.44e-44 159
Msa1405050 AT5G22890.1 31.667 180 106 5 46 210 174 351 3.27e-23 101
Msa1405050 AT1G34370.3 33.951 162 85 4 70 213 14 171 5.44e-23 99.8
Msa1405050 AT1G34370.2 33.544 158 91 3 70 213 224 381 7.34e-23 102
Msa1405050 AT1G34370.1 33.544 158 91 3 70 213 224 381 7.34e-23 102
Msa1405050 AT1G13290.1 34.591 159 84 7 72 218 89 239 1.07e-18 87.4
Msa1405050 AT3G57670.1 32.370 173 91 7 72 226 205 369 1.71e-18 87.8
Msa1405050 AT3G20880.1 33.125 160 91 5 84 228 250 408 1.83e-18 88.2
Msa1405050 AT1G51220.1 33.113 151 85 5 72 213 166 309 4.79e-18 85.9
Msa1405050 AT1G08290.1 35.294 153 80 7 72 213 168 312 5.36e-17 82.8
Msa1405050 AT1G34790.1 32.667 150 89 5 74 213 129 276 7.89e-16 79.0

Find 141 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTTGTACCACATATTT+TGG 0.125409 tig0023097:+77249 None:intergenic
AACATTTGAATACACATTTA+TGG 0.140693 tig0023097:+74349 None:intergenic
TTCGGTGCAGTTGAAGATTT+TGG 0.237456 tig0023097:+77497 None:intergenic
ACATAGCCAAATTAAGAAAA+AGG 0.238010 tig0023097:+78629 None:intergenic
TGATATGTTTAATGCTATAT+TGG 0.247245 tig0023097:-74940 Msa1405050:CDS
ATCAAATCATTGATATTAAT+TGG 0.250521 tig0023097:-74439 Msa1405050:three_prime_UTR
TACTATTATTAACTATTTCT+TGG 0.251790 tig0023097:+74568 None:intergenic
CCCATTATTAACCCTCAAAT+TGG 0.254900 tig0023097:-75440 Msa1405050:CDS
AACAACATCTTGGAAGAAAA+TGG 0.256543 tig0023097:-75245 Msa1405050:CDS
AATGCAAATGGGATAATAAA+TGG 0.269963 tig0023097:-74987 Msa1405050:CDS
CCTCATCTCCACACCATAAA+AGG 0.270952 tig0023097:-78843 Msa1405050:five_prime_UTR
ACCAATTTGAGGGTTAATAA+TGG 0.277668 tig0023097:+75439 None:intergenic
ACATTTGAATACACATTTAT+GGG 0.280310 tig0023097:+74350 None:intergenic
AGATTCAAATCCTCCATTCT+TGG 0.287816 tig0023097:+74641 None:intergenic
ATGTTGTTGTTGAAGGGTTT+TGG 0.296073 tig0023097:+75335 None:intergenic
CCAATTTGAGGGTTAATAAT+GGG 0.297467 tig0023097:+75440 None:intergenic
GGGTTAATAATGGGGTTTAA+AGG 0.300726 tig0023097:+75449 None:intergenic
TTTGAAAACACCACTTAGTT+TGG 0.303887 tig0023097:-74599 Msa1405050:exon
GTGAAGTTGCAGCAGATAAA+TGG 0.308637 tig0023097:+75216 None:intergenic
AATAAATTACCTGGATTTCC+TGG 0.317784 tig0023097:+78412 None:intergenic
AATAATAATTTCAATGCAAA+TGG 0.327404 tig0023097:-74999 Msa1405050:CDS
CTGATGAGGTTTGGAGAAAT+TGG 0.331929 tig0023097:-74668 Msa1405050:CDS
TTTGTAGCCATTAGTGTTGT+TGG 0.339715 tig0023097:+77562 None:intergenic
ACTCTTGTCTTGAGAAACTA+TGG 0.354103 tig0023097:+78718 None:intergenic
CAGCAGCAGCTGATGAGGTT+TGG 0.362969 tig0023097:-74677 Msa1405050:CDS
CAAAGCATTAATAGTGTTGT+TGG 0.386850 tig0023097:-74870 Msa1405050:CDS
AAAATACAAGAAACAGGCTT+TGG 0.391584 tig0023097:+78882 None:intergenic
GGTGCACATAGTAATTTCTA+TGG 0.395859 tig0023097:-74738 Msa1405050:CDS
CTTAATTGAAACAGAAAAGA+TGG 0.398347 tig0023097:+78777 None:intergenic
AGATCACCAAGTGCTCTTGC+AGG 0.399590 tig0023097:+77376 None:intergenic
GTTGTTGGAGGTGGAGGAGG+AGG 0.400532 tig0023097:-74855 Msa1405050:CDS
GGAGGAGGCGTTGGAATGAA+TGG 0.403069 tig0023097:-74966 Msa1405050:CDS
AAACACCACTTAGTTTGGTT+GGG 0.406309 tig0023097:-74594 Msa1405050:three_prime_UTR
TGTGTATTCAAATGTTATAA+TGG 0.406710 tig0023097:-74342 Msa1405050:three_prime_UTR
GCTACAGAAAGCTGCTCAAA+TGG 0.409352 tig0023097:-75171 Msa1405050:CDS
TGCTATATTGGATCAAAGTA+AGG 0.411463 tig0023097:-74928 Msa1405050:CDS
AATAATGTTATGAACATTAG+TGG 0.411466 tig0023097:-74816 Msa1405050:CDS
TGGAGATGAGGGGTGCTTGT+TGG 0.412864 tig0023097:+78855 None:intergenic
TATGAACATTAGTGGAAGTA+AGG 0.415131 tig0023097:-74808 Msa1405050:CDS
AATAGTGTTGTTGGAGGTGG+AGG 0.417136 tig0023097:-74861 Msa1405050:CDS
AATATGCTGTTCAATCTGAT+TGG 0.427517 tig0023097:-77282 Msa1405050:CDS
AGTGAATATGTTGTTGTTGA+AGG 0.428537 tig0023097:+75328 None:intergenic
AGTGTTGTTGGAGGTGGAGG+AGG 0.433876 tig0023097:-74858 Msa1405050:CDS
AGAGCACTTGGTGATCTTAC+TGG 0.434819 tig0023097:-77370 Msa1405050:CDS
ATAAAAGGAGGAGAGTTTCC+TGG 0.442673 tig0023097:-78828 Msa1405050:five_prime_UTR
AAAACACCACTTAGTTTGGT+TGG 0.442676 tig0023097:-74595 Msa1405050:three_prime_UTR
ATGATGGCGCCGCCGCAGTT+TGG 0.444697 tig0023097:-75086 Msa1405050:CDS
AACAGAAAAGATGGGATTTG+TGG 0.453016 tig0023097:+78786 None:intergenic
CAAGTGCTCTTGCAGGGTTA+TGG 0.453384 tig0023097:+77383 None:intergenic
TGGTGAACACATGAAGGTTC+AGG 0.454246 tig0023097:+77403 None:intergenic
AACCACCTCTTAAGAAGAAA+AGG 0.456615 tig0023097:-78441 Msa1405050:CDS
CAACAAACTGATCAAATACT+TGG 0.458193 tig0023097:-78538 Msa1405050:CDS
AGTTCATGAGGTGATGATAA+TGG 0.461625 tig0023097:+75374 None:intergenic
GAGGAAACGAGATTTGAGAT+TGG 0.462517 tig0023097:+75476 None:intergenic
ACTATCACCAACAACACTAA+TGG 0.464691 tig0023097:-77569 Msa1405050:CDS
AAGGCAAGTTGTGTCCTCTT+CGG 0.471989 tig0023097:+77479 None:intergenic
TGTAATGACACTGGATTTGT+TGG 0.477727 tig0023097:-74775 Msa1405050:CDS
ATAATAAATGGAGGAGGCGT+TGG 0.478927 tig0023097:-74975 Msa1405050:CDS
AAACGAGATTTGAGATTGGT+TGG 0.484142 tig0023097:+75480 None:intergenic
AGAAATTGGTCAACCAAGAA+TGG 0.485147 tig0023097:-74654 Msa1405050:CDS
GATTTGTTGGGGATAGGAGG+AGG 0.488522 tig0023097:-74762 Msa1405050:CDS
TAATTCACTCAACAACATCT+TGG 0.492063 tig0023097:-75255 Msa1405050:CDS
AGGAACCTACCAGGAAATCC+AGG 0.493855 tig0023097:-78421 Msa1405050:intron
CTGGATTTGTTGGGGATAGG+AGG 0.495376 tig0023097:-74765 Msa1405050:CDS
GATACAGTTCCACCAATTTG+AGG 0.499903 tig0023097:+75428 None:intergenic
CATAACCCTGCAAGAGCACT+TGG 0.500225 tig0023097:-77382 Msa1405050:CDS
ATACAGTTCCACCAATTTGA+GGG 0.501003 tig0023097:+75429 None:intergenic
ATTATTAACCCTCAAATTGG+TGG 0.502270 tig0023097:-75437 Msa1405050:CDS
GTGTCATGGTCGAAAGGAAG+GGG 0.506196 tig0023097:+74462 None:intergenic
AGTGCTATCACTTCAGCACT+TGG 0.507626 tig0023097:+77589 None:intergenic
CTACAGAAAGCTGCTCAAAT+GGG 0.511542 tig0023097:-75170 Msa1405050:CDS
AACACCACTTAGTTTGGTTG+GGG 0.514202 tig0023097:-74593 Msa1405050:three_prime_UTR
ATTAATAGTGTTGTTGGAGG+TGG 0.514406 tig0023097:-74864 Msa1405050:CDS
CGCCATCATGTTTGTAGCAA+CGG 0.514892 tig0023097:+75100 None:intergenic
TTTGAACTGAGTTGAAGAGA+AGG 0.515296 tig0023097:+75278 None:intergenic
AACATCACAAGTTGTGATAG+TGG 0.515608 tig0023097:-78604 Msa1405050:CDS
ATGTGTCATGGTCGAAAGGA+AGG 0.516071 tig0023097:+74460 None:intergenic
GTTGTTGCTGTTGCATGTAG+TGG 0.516364 tig0023097:+75030 None:intergenic
GTAATGACACTGGATTTGTT+GGG 0.517404 tig0023097:-74774 Msa1405050:CDS
GAGATGAAGTTGATGATGAA+AGG 0.518078 tig0023097:+75300 None:intergenic
GTGAATATGTTGTTGTTGAA+GGG 0.519299 tig0023097:+75329 None:intergenic
AATTACCTGGATTTCCTGGT+AGG 0.520896 tig0023097:+78416 None:intergenic
ATTCCAAAATATGTGGTACA+AGG 0.520937 tig0023097:-77252 Msa1405050:CDS
TTAATTGAAACAGAAAAGAT+GGG 0.527878 tig0023097:+78778 None:intergenic
GAAGAGGACACAACTTGCCT+TGG 0.527889 tig0023097:-77477 Msa1405050:CDS
ATAATAATTTCAATGCAAAT+GGG 0.528123 tig0023097:-74998 Msa1405050:CDS
ACATACATACCTGGAAAAGA+TGG 0.528499 tig0023097:+77207 None:intergenic
AACATTAGTGGAAGTAAGGG+TGG 0.535289 tig0023097:-74804 Msa1405050:CDS
CACATAGTAATTTCTATGGA+GGG 0.535940 tig0023097:-74734 Msa1405050:CDS
TTTGTTGAAGTATGAGAATG+TGG 0.537355 tig0023097:+78502 None:intergenic
ACTGTGAGTAATAATAATGC+AGG 0.538175 tig0023097:-75143 Msa1405050:CDS
CAATTTGAGGGTTAATAATG+GGG 0.543880 tig0023097:+75441 None:intergenic
CTAGTGCAGAAATTAGAAAG+AGG 0.551088 tig0023097:-77438 Msa1405050:CDS
AATGGGGTTTAAAGGTAATG+AGG 0.552566 tig0023097:+75457 None:intergenic
ATTAGTGGAAGTAAGGGTGG+TGG 0.553858 tig0023097:-74801 Msa1405050:CDS
AGCATTAATAGTGTTGTTGG+AGG 0.564315 tig0023097:-74867 Msa1405050:CDS
GCACATAGTAATTTCTATGG+AGG 0.570101 tig0023097:-74735 Msa1405050:CDS
TTGTTGGGGATAGGAGGAGG+AGG 0.571045 tig0023097:-74759 Msa1405050:CDS
TGCGTCACAGAATGCTCTGT+GGG 0.571848 tig0023097:+75556 None:intergenic
AAAGCTCATTCCAAAATATG+TGG 0.572953 tig0023097:-77259 Msa1405050:CDS
ACACTGGATTTGTTGGGGAT+AGG 0.573986 tig0023097:-74768 Msa1405050:CDS
TAGTGCAGAAATTAGAAAGA+GGG 0.575321 tig0023097:-77437 Msa1405050:CDS
TACCGTTGCTACAAACATGA+TGG 0.575806 tig0023097:-75102 Msa1405050:CDS
CATAGTAATTTCTATGGAGG+GGG 0.576896 tig0023097:-74732 Msa1405050:CDS
CGTATGTGAAATATGCAACA+AGG 0.589045 tig0023097:-77533 Msa1405050:CDS
ATGAACATTAGTGGAAGTAA+GGG 0.589532 tig0023097:-74807 Msa1405050:CDS
TGTGTCATGGTCGAAAGGAA+GGG 0.590566 tig0023097:+74461 None:intergenic
ACATAGTAATTTCTATGGAG+GGG 0.591190 tig0023097:-74733 Msa1405050:CDS
GATCACCAAGTGCTCTTGCA+GGG 0.594376 tig0023097:+77377 None:intergenic
TGTCATGGTCGAAAGGAAGG+GGG 0.594893 tig0023097:+74463 None:intergenic
ACATCACAAGTTGTGATAGT+GGG 0.595444 tig0023097:-78603 Msa1405050:CDS
GGGTTATGGTGAACACATGA+AGG 0.595455 tig0023097:+77397 None:intergenic
AAGAAGAAAAGGAACCTACC+AGG 0.596110 tig0023097:-78430 Msa1405050:CDS
ATCAATGATTTGATGTGTCA+TGG 0.599292 tig0023097:+74448 None:intergenic
GGCTCCCCAACCAAACTAAG+TGG 0.601479 tig0023097:+74589 None:intergenic
AGCTTTCTGTAGCAATGCAG+TGG 0.610000 tig0023097:+75181 None:intergenic
TTCCAAAATATGTGGTACAA+GGG 0.611826 tig0023097:-77251 Msa1405050:CDS
ACAAGCTATCTAGGAAGCAG+AGG 0.613878 tig0023097:+78751 None:intergenic
ATGGAAGCTTGTGATCTGAA+TGG 0.617097 tig0023097:+75393 None:intergenic
AATCTTCAACTGCACCGAAG+AGG 0.621618 tig0023097:-77493 Msa1405050:CDS
TTATGTGTAGACTCTTATGA+TGG 0.635701 tig0023097:-74374 Msa1405050:three_prime_UTR
TAATGACACTGGATTTGTTG+GGG 0.637157 tig0023097:-74773 Msa1405050:CDS
AATTGGTCAACCAAGAATGG+AGG 0.638927 tig0023097:-74651 Msa1405050:CDS
GTATGTGAAATATGCAACAA+GGG 0.639897 tig0023097:-77532 Msa1405050:CDS
AATGGGATAATAAATGGAGG+AGG 0.644754 tig0023097:-74981 Msa1405050:CDS
TGGTGGAGATGTAATGACAC+TGG 0.649106 tig0023097:-74784 Msa1405050:CDS
GTCATGGTCGAAAGGAAGGG+GGG 0.655496 tig0023097:+74464 None:intergenic
TGCACTACACCAAACTGCGG+CGG 0.655717 tig0023097:+75077 None:intergenic
GCAAATGGGATAATAAATGG+AGG 0.656300 tig0023097:-74984 Msa1405050:CDS
TCTACACATAACACTTACAC+TGG 0.657671 tig0023097:+74386 None:intergenic
GATGGGATTTGTGGAACCAG+AGG 0.657823 tig0023097:+78795 None:intergenic
TTTGATGTGTCATGGTCGAA+AGG 0.659881 tig0023097:+74456 None:intergenic
TAATAATGCAGGTATCATGA+CGG 0.660595 tig0023097:-75132 Msa1405050:CDS
CATCTCCACACCATAAAAGG+AGG 0.660797 tig0023097:-78840 Msa1405050:five_prime_UTR
AGGGAATACAAATGTGACTG+TGG 0.667636 tig0023097:-77232 Msa1405050:CDS
ATGCGTCACAGAATGCTCTG+TGG 0.669753 tig0023097:+75555 None:intergenic
TTTGGTGTAGTGCAACAACA+AGG 0.680990 tig0023097:-75068 Msa1405050:CDS
GAGAACAACAAAGCAAACGA+AGG 0.700937 tig0023097:-75524 Msa1405050:CDS
ACTACTGACATGAGTTCATG+AGG 0.716939 tig0023097:+75362 None:intergenic
TGTTGCACTACACCAAACTG+CGG 0.718918 tig0023097:+75074 None:intergenic
AATGCAGTGGCTGACATGTG+AGG 0.731584 tig0023097:+75194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAACTAAATAGAAAAAAA+AGG - tig0023097:74558-74577 Msa1405050:three_prime_UTR 10.0%
!! TAAACTAAATAGAAAAAAAA+GGG - tig0023097:74559-74578 Msa1405050:three_prime_UTR 10.0%
!! AATAATAATAATAATAGCAA+TGG - tig0023097:74745-74764 Msa1405050:CDS 10.0%
!! TAAAATAAATGATGAATTAT+TGG - tig0023097:75457-75476 Msa1405050:CDS 10.0%
!! TAAAAGAAAAAAGATATAAA+AGG + tig0023097:76538-76557 None:intergenic 10.0%
!!! TTTTATTTTTAAAGATAAGT+TGG - tig0023097:76746-76765 Msa1405050:intron 10.0%
!! AACATAAATGTAAATTATTT+GGG + tig0023097:76867-76886 None:intergenic 10.0%
!! AAACATAAATGTAAATTATT+TGG + tig0023097:76868-76887 None:intergenic 10.0%
!! TTAAGAAAATGTATAAATAA+AGG + tig0023097:77387-77406 None:intergenic 10.0%
!! CAATATGCAAATAAAATTAA+AGG + tig0023097:74899-74918 None:intergenic 15.0%
!! TGTTACATAATTAAAATCAA+GGG + tig0023097:74937-74956 None:intergenic 15.0%
!! TTGTTACATAATTAAAATCA+AGG + tig0023097:74938-74957 None:intergenic 15.0%
!!! TACATAATTATTGTTATTCA+TGG - tig0023097:75025-75044 Msa1405050:CDS 15.0%
!!! ACATAATTATTGTTATTCAT+GGG - tig0023097:75026-75045 Msa1405050:CDS 15.0%
!!! TATTTTTTTTTACCTCATAT+TGG + tig0023097:75254-75273 None:intergenic 15.0%
!! TTTAATTAAGACATTTCTAT+AGG - tig0023097:75304-75323 Msa1405050:CDS 15.0%
!!! TTTTTAACTTTTTGTAGTTT+AGG - tig0023097:76251-76270 Msa1405050:intron 15.0%
!!! TTCTTTAAGAAATTGTATAA+TGG - tig0023097:76586-76605 Msa1405050:intron 15.0%
!! CTAATTAATAATAAGAGTTT+AGG - tig0023097:76639-76658 Msa1405050:intron 15.0%
!! TAAAGAAAAATACTTACTTA+GGG - tig0023097:76680-76699 Msa1405050:intron 15.0%
!!! TCTTCATTGTTTTTTTTTTA+TGG - tig0023097:76815-76834 Msa1405050:intron 15.0%
!!! GCAATTTGTTATTTTTTTTA+AGG + tig0023097:77047-77066 None:intergenic 15.0%
!! AATAAAGGAATATATTGTAT+TGG + tig0023097:77372-77391 None:intergenic 15.0%
!! ATATATTATAATTACTCATG+AGG + tig0023097:77532-77551 None:intergenic 15.0%
!! AATAATAATTTCAATGCAAA+TGG - tig0023097:78227-78246 Msa1405050:intron 15.0%
!! ATAATAATTTCAATGCAAAT+GGG - tig0023097:78228-78247 Msa1405050:intron 15.0%
!!! TACTATTATTAACTATTTCT+TGG + tig0023097:78661-78680 None:intergenic 15.0%
!! ATCAAATCATTGATATTAAT+TGG - tig0023097:78787-78806 Msa1405050:five_prime_UTR 15.0%
!! TTAATTGAAACAGAAAAGAT+GGG + tig0023097:74451-74470 None:intergenic 20.0%
!! AGAATACAAAATAAATTACC+TGG + tig0023097:74826-74845 None:intergenic 20.0%
!!! TTTTTGTGAGATATCATTTT+TGG + tig0023097:75229-75248 None:intergenic 20.0%
!! AAAAATGATATCTCACAAAA+AGG - tig0023097:75228-75247 Msa1405050:CDS 20.0%
!! AGGTAAAAAAAAATAGACAA+TGG - tig0023097:75259-75278 Msa1405050:CDS 20.0%
!! CTTCACTTAAAAGAATAATT+AGG + tig0023097:75379-75398 None:intergenic 20.0%
!!! AATAACGTGTTTAATTTGTT+TGG - tig0023097:75607-75626 Msa1405050:intron 20.0%
!! GTAAAGAAAAATACTTACTT+AGG - tig0023097:76679-76698 Msa1405050:intron 20.0%
!! TGATATGTTTAATGCTATAT+TGG - tig0023097:78286-78305 Msa1405050:intron 20.0%
!! AATAATGTTATGAACATTAG+TGG - tig0023097:78410-78429 Msa1405050:intron 20.0%
!!! TATTCAACTTTTTGAATTAG+TGG - tig0023097:78679-78698 Msa1405050:five_prime_UTR 20.0%
!!! ATTCAACTTTTTGAATTAGT+GGG - tig0023097:78680-78699 Msa1405050:five_prime_UTR 20.0%
!! ACATTTGAATACACATTTAT+GGG + tig0023097:78879-78898 None:intergenic 20.0%
!! AACATTTGAATACACATTTA+TGG + tig0023097:78880-78899 None:intergenic 20.0%
!! TGTGTATTCAAATGTTATAA+TGG - tig0023097:78884-78903 Msa1405050:five_prime_UTR 20.0%
! CTTAATTGAAACAGAAAAGA+TGG + tig0023097:74452-74471 None:intergenic 25.0%
! AAGAAAAAAACAAGCTATCT+AGG + tig0023097:74487-74506 None:intergenic 25.0%
!! CTACTTCCTTTTTCTTAATT+TGG - tig0023097:74591-74610 Msa1405050:three_prime_UTR 25.0%
! ACATAGCCAAATTAAGAAAA+AGG + tig0023097:74600-74619 None:intergenic 25.0%
! TTGTTATAGAATATGATGCT+TGG + tig0023097:75072-75091 None:intergenic 25.0%
!! TTTAGAATTAACCAATGAGA+AGG + tig0023097:75147-75166 None:intergenic 25.0%
!! AGTCTTATCCTTTAATTTCA+TGG + tig0023097:75332-75351 None:intergenic 25.0%
! AAGACAAAGATCTATATAGT+GGG - tig0023097:75396-75415 Msa1405050:CDS 25.0%
! GTGTGAAACAAAGAAAAAAT+AGG + tig0023097:75568-75587 None:intergenic 25.0%
! GAAATGTGAATAAAAACTTG+TGG - tig0023097:76044-76063 Msa1405050:intron 25.0%
! TAGAATATCATTGCTCAAAA+AGG + tig0023097:76343-76362 None:intergenic 25.0%
!!! TCACAAAAGTTGTTGATTTT+TGG + tig0023097:76370-76389 None:intergenic 25.0%
!!! TCTACTTTTCCTTTGAAAAT+TGG + tig0023097:76399-76418 None:intergenic 25.0%
!! ACTTTCTCTTTCTATTGTTT+TGG + tig0023097:76436-76455 None:intergenic 25.0%
! AAACACAGATGTAAAATGTT+TGG - tig0023097:76705-76724 Msa1405050:intron 25.0%
!! ATATGAATGATGATGATTTG+CGG + tig0023097:76931-76950 None:intergenic 25.0%
! GTAACTTATAATTGTACTCA+AGG + tig0023097:77233-77252 None:intergenic 25.0%
! ATTAATAAGAAACACTAGCT+AGG + tig0023097:77611-77630 None:intergenic 25.0%
! AATGCAAATGGGATAATAAA+TGG - tig0023097:78239-78258 Msa1405050:intron 25.0%
!! TTCAACTTTTTGAATTAGTG+GGG - tig0023097:78681-78700 Msa1405050:five_prime_UTR 25.0%
AAAATACAAGAAACAGGCTT+TGG + tig0023097:74347-74366 None:intergenic 30.0%
GGCATAAAAATACAAGAAAC+AGG + tig0023097:74353-74372 None:intergenic 30.0%
CAACAAACTGATCAAATACT+TGG - tig0023097:74688-74707 Msa1405050:CDS 30.0%
TTTGTTGAAGTATGAGAATG+TGG + tig0023097:74727-74746 None:intergenic 30.0%
AATAAATTACCTGGATTTCC+TGG + tig0023097:74817-74836 None:intergenic 30.0%
TAGAATATGATGCTTGGAAT+TGG + tig0023097:75066-75085 None:intergenic 30.0%
!! TTTTGGAAACGAAGATCATT+TGG + tig0023097:75212-75231 None:intergenic 30.0%
TATAGGCTCCATGAAATTAA+AGG - tig0023097:75321-75340 Msa1405050:CDS 30.0%
TTAAAGGATAAGACTTGATC+TGG - tig0023097:75337-75356 Msa1405050:CDS 30.0%
GAAGACAAAGATCTATATAG+TGG - tig0023097:75395-75414 Msa1405050:CDS 30.0%
AGACAAAGATCTATATAGTG+GGG - tig0023097:75397-75416 Msa1405050:CDS 30.0%
CAACAGTGTACAACAATTAT+TGG + tig0023097:75532-75551 None:intergenic 30.0%
GTATGTGAAATATGCAACAA+GGG - tig0023097:75694-75713 Msa1405050:intron 30.0%
TAGTGCAGAAATTAGAAAGA+GGG - tig0023097:75789-75808 Msa1405050:intron 30.0%
AATATGCTGTTCAATCTGAT+TGG - tig0023097:75944-75963 Msa1405050:intron 30.0%
AAAGCTCATTCCAAAATATG+TGG - tig0023097:75967-75986 Msa1405050:intron 30.0%
ATTCCAAAATATGTGGTACA+AGG - tig0023097:75974-75993 Msa1405050:intron 30.0%
TTCCAAAATATGTGGTACAA+GGG - tig0023097:75975-75994 Msa1405050:intron 30.0%
CGCACACACTTTATTATTAT+TGG + tig0023097:76147-76166 None:intergenic 30.0%
AGCTAAGAATAAGATAGACT+GGG - tig0023097:76179-76198 Msa1405050:intron 30.0%
!! GAAAAGTGTTCTTAGTTCAT+AGG + tig0023097:76218-76237 None:intergenic 30.0%
!! ACTTTTCCTTTGAAAATTGG+TGG + tig0023097:76396-76415 None:intergenic 30.0%
GTATATTATAATACCCATGC+AGG - tig0023097:77505-77524 Msa1405050:CDS 30.0%
CAATTTGAGGGTTAATAATG+GGG + tig0023097:77788-77807 None:intergenic 30.0%
CCAATTTGAGGGTTAATAAT+GGG + tig0023097:77789-77808 None:intergenic 30.0%
ACCAATTTGAGGGTTAATAA+TGG + tig0023097:77790-77809 None:intergenic 30.0%
ATTATTAACCCTCAAATTGG+TGG - tig0023097:77789-77808 Msa1405050:intron 30.0%
! GTGAATATGTTGTTGTTGAA+GGG + tig0023097:77900-77919 None:intergenic 30.0%
! AGTGAATATGTTGTTGTTGA+AGG + tig0023097:77901-77920 None:intergenic 30.0%
TAATTCACTCAACAACATCT+TGG - tig0023097:77971-77990 Msa1405050:intron 30.0%
AACAACATCTTGGAAGAAAA+TGG - tig0023097:77981-78000 Msa1405050:intron 30.0%
ACTGTGAGTAATAATAATGC+AGG - tig0023097:78083-78102 Msa1405050:intron 30.0%
TAATAATGCAGGTATCATGA+CGG - tig0023097:78094-78113 Msa1405050:intron 30.0%
TGCTATATTGGATCAAAGTA+AGG - tig0023097:78298-78317 Msa1405050:intron 30.0%
! CAAAGCATTAATAGTGTTGT+TGG - tig0023097:78356-78375 Msa1405050:intron 30.0%
TATGAACATTAGTGGAAGTA+AGG - tig0023097:78418-78437 Msa1405050:intron 30.0%
ATGAACATTAGTGGAAGTAA+GGG - tig0023097:78419-78438 Msa1405050:intron 30.0%
CACATAGTAATTTCTATGGA+GGG - tig0023097:78492-78511 Msa1405050:CDS 30.0%
ACATAGTAATTTCTATGGAG+GGG - tig0023097:78493-78512 Msa1405050:CDS 30.0%
TTTGAAAACACCACTTAGTT+TGG - tig0023097:78627-78646 Msa1405050:exon 30.0%
ATCAATGATTTGATGTGTCA+TGG + tig0023097:78781-78800 None:intergenic 30.0%
TTATGTGTAGACTCTTATGA+TGG - tig0023097:78852-78871 Msa1405050:five_prime_UTR 30.0%
AACAGAAAAGATGGGATTTG+TGG + tig0023097:74443-74462 None:intergenic 35.0%
ACTCTTGTCTTGAGAAACTA+TGG + tig0023097:74511-74530 None:intergenic 35.0%
AACATCACAAGTTGTGATAG+TGG - tig0023097:74622-74641 Msa1405050:CDS 35.0%
ACATCACAAGTTGTGATAGT+GGG - tig0023097:74623-74642 Msa1405050:CDS 35.0%
AACCACCTCTTAAGAAGAAA+AGG - tig0023097:74785-74804 Msa1405050:CDS 35.0%
! TTCCTTTTCTTCTTAAGAGG+TGG + tig0023097:74790-74809 None:intergenic 35.0%
! AGGTTCCTTTTCTTCTTAAG+AGG + tig0023097:74793-74812 None:intergenic 35.0%
ACTTCTTTCAACCTTCTCAT+TGG - tig0023097:75133-75152 Msa1405050:CDS 35.0%
ACTATCACCAACAACACTAA+TGG - tig0023097:75657-75676 Msa1405050:intron 35.0%
TTTGTAGCCATTAGTGTTGT+TGG + tig0023097:75667-75686 None:intergenic 35.0%
CGTATGTGAAATATGCAACA+AGG - tig0023097:75693-75712 Msa1405050:intron 35.0%
!!! GTTCTTTGTTTTAGCTTCCA+AGG + tig0023097:75769-75788 None:intergenic 35.0%
CTAGTGCAGAAATTAGAAAG+AGG - tig0023097:75788-75807 Msa1405050:intron 35.0%
! AAGCACTTTTGTAGAAAGCA+TGG - tig0023097:75886-75905 Msa1405050:intron 35.0%
! TTCCCTTGTACCACATATTT+TGG + tig0023097:75980-75999 None:intergenic 35.0%
ACATACATACCTGGAAAAGA+TGG + tig0023097:76022-76041 None:intergenic 35.0%
ACATTTCACACATACATACC+TGG + tig0023097:76031-76050 None:intergenic 35.0%
CTTATTCTTAGCTGCCAAAA+CGG + tig0023097:76172-76191 None:intergenic 35.0%
CAGCTAAGAATAAGATAGAC+TGG - tig0023097:76178-76197 Msa1405050:intron 35.0%
! TGACAACCACCAATTTTCAA+AGG - tig0023097:76387-76406 Msa1405050:intron 35.0%
GAGAGATAAAGTTGTCACAT+AGG - tig0023097:76475-76494 Msa1405050:intron 35.0%
TCACATAGGTTGTCACAAAA+TGG - tig0023097:76489-76508 Msa1405050:intron 35.0%
TCAAAGATTCCTACGTATGT+AGG - tig0023097:77456-77475 Msa1405050:CDS 35.0%
CAAAGATTCCTACGTATGTA+GGG - tig0023097:77457-77476 Msa1405050:CDS 35.0%
TTTATACACCCTACATACGT+AGG + tig0023097:77468-77487 None:intergenic 35.0%
! AAACGAGATTTGAGATTGGT+TGG + tig0023097:77749-77768 None:intergenic 35.0%
AATGGGGTTTAAAGGTAATG+AGG + tig0023097:77772-77791 None:intergenic 35.0%
! GGGTTAATAATGGGGTTTAA+AGG + tig0023097:77780-77799 None:intergenic 35.0%
CCCATTATTAACCCTCAAAT+TGG - tig0023097:77786-77805 Msa1405050:intron 35.0%
ATACAGTTCCACCAATTTGA+GGG + tig0023097:77800-77819 None:intergenic 35.0%
AGTTCATGAGGTGATGATAA+TGG + tig0023097:77855-77874 None:intergenic 35.0%
!! ATGTTGTTGTTGAAGGGTTT+TGG + tig0023097:77894-77913 None:intergenic 35.0%
!! GAGATGAAGTTGATGATGAA+AGG + tig0023097:77929-77948 None:intergenic 35.0%
! TTTGAACTGAGTTGAAGAGA+AGG + tig0023097:77951-77970 None:intergenic 35.0%
GCAAATGGGATAATAAATGG+AGG - tig0023097:78242-78261 Msa1405050:intron 35.0%
AATGGGATAATAAATGGAGG+AGG - tig0023097:78245-78264 Msa1405050:intron 35.0%
! AGCATTAATAGTGTTGTTGG+AGG - tig0023097:78359-78378 Msa1405050:intron 35.0%
! ATTAATAGTGTTGTTGGAGG+TGG - tig0023097:78362-78381 Msa1405050:intron 35.0%
TGTAATGACACTGGATTTGT+TGG - tig0023097:78451-78470 Msa1405050:CDS 35.0%
! GTAATGACACTGGATTTGTT+GGG - tig0023097:78452-78471 Msa1405050:CDS 35.0%
!! TAATGACACTGGATTTGTTG+GGG - tig0023097:78453-78472 Msa1405050:CDS 35.0%
GGTGCACATAGTAATTTCTA+TGG - tig0023097:78488-78507 Msa1405050:CDS 35.0%
GCACATAGTAATTTCTATGG+AGG - tig0023097:78491-78510 Msa1405050:CDS 35.0%
CATAGTAATTTCTATGGAGG+GGG - tig0023097:78494-78513 Msa1405050:CDS 35.0%
AGAAATTGGTCAACCAAGAA+TGG - tig0023097:78572-78591 Msa1405050:CDS 35.0%
AGATTCAAATCCTCCATTCT+TGG + tig0023097:78588-78607 None:intergenic 35.0%
! AAAACACCACTTAGTTTGGT+TGG - tig0023097:78631-78650 Msa1405050:exon 35.0%
! AAACACCACTTAGTTTGGTT+GGG - tig0023097:78632-78651 Msa1405050:exon 35.0%
TCTACACATAACACTTACAC+TGG + tig0023097:78843-78862 None:intergenic 35.0%
! TTTTATGGTGTGGAGATGAG+GGG + tig0023097:74384-74403 None:intergenic 40.0%
! CTTTTATGGTGTGGAGATGA+GGG + tig0023097:74385-74404 None:intergenic 40.0%
! AGGAAACTCTCCTCCTTTTA+TGG + tig0023097:74399-74418 None:intergenic 40.0%
ATAAAAGGAGGAGAGTTTCC+TGG - tig0023097:74398-74417 Msa1405050:three_prime_UTR 40.0%
AAGAAGAAAAGGAACCTACC+AGG - tig0023097:74796-74815 Msa1405050:CDS 40.0%
AATTACCTGGATTTCCTGGT+AGG + tig0023097:74813-74832 None:intergenic 40.0%
CTCACAAAAAGGCCAATATG+AGG - tig0023097:75239-75258 Msa1405050:CDS 40.0%
GTGTGTGTAAGTAAGTAGCT+AGG - tig0023097:75479-75498 Msa1405050:CDS 40.0%
!! TTCGGTGCAGTTGAAGATTT+TGG + tig0023097:75732-75751 None:intergenic 40.0%
! GAAAGCATGGTGAGAAAAAG+TGG - tig0023097:75899-75918 Msa1405050:intron 40.0%
AGGGAATACAAATGTGACTG+TGG - tig0023097:75994-76013 Msa1405050:intron 40.0%
GTTCTTAGTTCATAGGTGTG+TGG + tig0023097:76211-76230 None:intergenic 40.0%
CGGTGTAGAGCATATTAAAG+TGG + tig0023097:76911-76930 None:intergenic 40.0%
GAGAACAACAAAGCAAACGA+AGG - tig0023097:77702-77721 Msa1405050:intron 40.0%
! GAGGAAACGAGATTTGAGAT+TGG + tig0023097:77753-77772 None:intergenic 40.0%
GATACAGTTCCACCAATTTG+AGG + tig0023097:77801-77820 None:intergenic 40.0%
ATGGAAGCTTGTGATCTGAA+TGG + tig0023097:77836-77855 None:intergenic 40.0%
ACTACTGACATGAGTTCATG+AGG + tig0023097:77867-77886 None:intergenic 40.0%
!! TTGTTGTTGAAGGGTTTTGG+TGG + tig0023097:77891-77910 None:intergenic 40.0%
GTGAAGTTGCAGCAGATAAA+TGG + tig0023097:78013-78032 None:intergenic 40.0%
CTACAGAAAGCTGCTCAAAT+GGG - tig0023097:78056-78075 Msa1405050:intron 40.0%
TACCGTTGCTACAAACATGA+TGG - tig0023097:78124-78143 Msa1405050:intron 40.0%
! TTTGGTGTAGTGCAACAACA+AGG - tig0023097:78158-78177 Msa1405050:intron 40.0%
! ATAATAAATGGAGGAGGCGT+TGG - tig0023097:78251-78270 Msa1405050:intron 40.0%
AACATTAGTGGAAGTAAGGG+TGG - tig0023097:78422-78441 Msa1405050:CDS 40.0%
CTGATGAGGTTTGGAGAAAT+TGG - tig0023097:78558-78577 Msa1405050:CDS 40.0%
AATTGGTCAACCAAGAATGG+AGG - tig0023097:78575-78594 Msa1405050:CDS 40.0%
! AACACCACTTAGTTTGGTTG+GGG - tig0023097:78633-78652 Msa1405050:five_prime_UTR 40.0%
TTTGATGTGTCATGGTCGAA+AGG + tig0023097:78773-78792 None:intergenic 40.0%
! CCTTTTATGGTGTGGAGATG+AGG + tig0023097:74386-74405 None:intergenic 45.0%
CCTCATCTCCACACCATAAA+AGG - tig0023097:74383-74402 Msa1405050:three_prime_UTR 45.0%
CATCTCCACACCATAAAAGG+AGG - tig0023097:74386-74405 Msa1405050:three_prime_UTR 45.0%
! ACTCTCCTCCTTTTATGGTG+TGG + tig0023097:74394-74413 None:intergenic 45.0%
!! TCCTGGTTTTTGTCTTCCTC+TGG - tig0023097:74415-74434 Msa1405050:three_prime_UTR 45.0%
ACCAGAGGAAGACAAAAACC+AGG + tig0023097:74419-74438 None:intergenic 45.0%
ACAAGCTATCTAGGAAGCAG+AGG + tig0023097:74478-74497 None:intergenic 45.0%
AGTGCTATCACTTCAGCACT+TGG + tig0023097:75640-75659 None:intergenic 45.0%
AATCTTCAACTGCACCGAAG+AGG - tig0023097:75733-75752 Msa1405050:intron 45.0%
AAGGCAAGTTGTGTCCTCTT+CGG + tig0023097:75750-75769 None:intergenic 45.0%
TGGTGAACACATGAAGGTTC+AGG + tig0023097:75826-75845 None:intergenic 45.0%
GGGTTATGGTGAACACATGA+AGG + tig0023097:75832-75851 None:intergenic 45.0%
! AGAGCACTTGGTGATCTTAC+TGG - tig0023097:75856-75875 Msa1405050:intron 45.0%
! ACTGTGGAACCATCTTTTCC+AGG - tig0023097:76010-76029 Msa1405050:intron 45.0%
TTACTCATGAGGACCTGCAT+GGG + tig0023097:77521-77540 None:intergenic 45.0%
ATTACTCATGAGGACCTGCA+TGG + tig0023097:77522-77541 None:intergenic 45.0%
AGCTTTCTGTAGCAATGCAG+TGG + tig0023097:78048-78067 None:intergenic 45.0%
GCTACAGAAAGCTGCTCAAA+TGG - tig0023097:78055-78074 Msa1405050:intron 45.0%
CGCCATCATGTTTGTAGCAA+CGG + tig0023097:78129-78148 None:intergenic 45.0%
TGTTGCACTACACCAAACTG+CGG + tig0023097:78155-78174 None:intergenic 45.0%
! GTTGTTGCTGTTGCATGTAG+TGG + tig0023097:78199-78218 None:intergenic 45.0%
! AATAGTGTTGTTGGAGGTGG+AGG - tig0023097:78365-78384 Msa1405050:intron 45.0%
ATTAGTGGAAGTAAGGGTGG+TGG - tig0023097:78425-78444 Msa1405050:CDS 45.0%
TGGTGGAGATGTAATGACAC+TGG - tig0023097:78442-78461 Msa1405050:CDS 45.0%
!! ACACTGGATTTGTTGGGGAT+AGG - tig0023097:78458-78477 Msa1405050:CDS 45.0%
TGTGTCATGGTCGAAAGGAA+GGG + tig0023097:78768-78787 None:intergenic 45.0%
ATGTGTCATGGTCGAAAGGA+AGG + tig0023097:78769-78788 None:intergenic 45.0%
!! TATATAGTAATAAATTTATT+TGG - tig0023097:77428-77447 Msa1405050:CDS 5.0%
GATGGGATTTGTGGAACCAG+AGG + tig0023097:74434-74453 None:intergenic 50.0%
AGGAACCTACCAGGAAATCC+AGG - tig0023097:74805-74824 Msa1405050:CDS 50.0%
GAAGAGGACACAACTTGCCT+TGG - tig0023097:75749-75768 Msa1405050:intron 50.0%
CAAGTGCTCTTGCAGGGTTA+TGG + tig0023097:75846-75865 None:intergenic 50.0%
!! CATAACCCTGCAAGAGCACT+TGG - tig0023097:75844-75863 Msa1405050:intron 50.0%
GATCACCAAGTGCTCTTGCA+GGG + tig0023097:75852-75871 None:intergenic 50.0%
AGATCACCAAGTGCTCTTGC+AGG + tig0023097:75853-75872 None:intergenic 50.0%
!!! AAAGTGTGTGCGTGCCGTTT+TGG - tig0023097:76155-76174 Msa1405050:intron 50.0%
TGCGTCACAGAATGCTCTGT+GGG + tig0023097:77673-77692 None:intergenic 50.0%
ATGCGTCACAGAATGCTCTG+TGG + tig0023097:77674-77693 None:intergenic 50.0%
AATGCAGTGGCTGACATGTG+AGG + tig0023097:78035-78054 None:intergenic 50.0%
!! CTGGATTTGTTGGGGATAGG+AGG - tig0023097:78461-78480 Msa1405050:CDS 50.0%
!! GATTTGTTGGGGATAGGAGG+AGG - tig0023097:78464-78483 Msa1405050:CDS 50.0%
! AGGGGGTACACAACAACAAC+AGG - tig0023097:78511-78530 Msa1405050:CDS 50.0%
TGTCATGGTCGAAAGGAAGG+GGG + tig0023097:78766-78785 None:intergenic 50.0%
GTGTCATGGTCGAAAGGAAG+GGG + tig0023097:78767-78786 None:intergenic 50.0%
!! TGGAGATGAGGGGTGCTTGT+TGG + tig0023097:74374-74393 None:intergenic 55.0%
TGCACTACACCAAACTGCGG+CGG + tig0023097:78152-78171 None:intergenic 55.0%
!! GGAGGAGGCGTTGGAATGAA+TGG - tig0023097:78260-78279 Msa1405050:intron 55.0%
! AGTGTTGTTGGAGGTGGAGG+AGG - tig0023097:78368-78387 Msa1405050:intron 55.0%
TTGTTGGGGATAGGAGGAGG+AGG - tig0023097:78467-78486 Msa1405050:CDS 55.0%
CAGCAGCAGCTGATGAGGTT+TGG - tig0023097:78549-78568 Msa1405050:CDS 55.0%
GGCTCCCCAACCAAACTAAG+TGG + tig0023097:78640-78659 None:intergenic 55.0%
GTCATGGTCGAAAGGAAGGG+GGG + tig0023097:78765-78784 None:intergenic 55.0%
GTTGTTGGAGGTGGAGGAGG+AGG - tig0023097:78371-78390 Msa1405050:intron 60.0%
AGCAGCAGCAGCAGCTGATG+AGG - tig0023097:78544-78563 Msa1405050:CDS 60.0%
! TCGAAAGGAAGGGGGGTGTG+GGG + tig0023097:78758-78777 None:intergenic 60.0%
! GTCGAAAGGAAGGGGGGTGT+GGG + tig0023097:78759-78778 None:intergenic 60.0%
! ATGATGGCGCCGCCGCAGTT+TGG - tig0023097:78140-78159 Msa1405050:intron 65.0%
GGTCGAAAGGAAGGGGGGTG+TGG + tig0023097:78760-78779 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0023097 gene 74326 78922 74326 ID=Msa1405050;Name=Msa1405050
tig0023097 mRNA 74326 78922 74326 ID=Msa1405050-mRNA-1;Parent=Msa1405050;Name=Msa1405050-mRNA-1;_AED=0.27;_eAED=0.27;_QI=290|1|1|1|1|1|3|291|528
tig0023097 exon 74326 75595 74326 ID=Msa1405050-mRNA-1:exon:23917;Parent=Msa1405050-mRNA-1
tig0023097 exon 77217 77613 77217 ID=Msa1405050-mRNA-1:exon:23916;Parent=Msa1405050-mRNA-1
tig0023097 exon 78422 78922 78422 ID=Msa1405050-mRNA-1:exon:23915;Parent=Msa1405050-mRNA-1
tig0023097 five_prime_UTR 78633 78922 78633 ID=Msa1405050-mRNA-1:five_prime_utr;Parent=Msa1405050-mRNA-1
tig0023097 CDS 78422 78632 78422 ID=Msa1405050-mRNA-1:cds;Parent=Msa1405050-mRNA-1
tig0023097 CDS 77217 77613 77217 ID=Msa1405050-mRNA-1:cds;Parent=Msa1405050-mRNA-1
tig0023097 CDS 74617 75595 74617 ID=Msa1405050-mRNA-1:cds;Parent=Msa1405050-mRNA-1
tig0023097 three_prime_UTR 74326 74616 74326 ID=Msa1405050-mRNA-1:three_prime_utr;Parent=Msa1405050-mRNA-1
Gene Sequence

>Msa1405050

ATGTCAAACATCACAAGTTGTGATAGTGGGAGTTTCTCTACAGAGAATACAAGAGAAGATGCAGTTAAACAGCAACAAACTGATCAAATACTTGGTCAATTTCATAGTCCACATTCTCATACTTCAACAAATAATAATAATAATAGCAATGGTTCTAACACAGATTTACAACCACCTCTTAAGAAGAAAAGGAACCTACCAGGAAATCCAGATCCAAGTGCTGAAGTGATAGCACTATCACCAACAACACTAATGGCTACAAACAGATTCGTATGTGAAATATGCAACAAGGGTTTTCAAAGAGACCAAAATCTTCAACTGCACCGAAGAGGACACAACTTGCCTTGGAAGCTAAAACAAAGAACTAGTGCAGAAATTAGAAAGAGGGTTTATGTTTGTCCTGAACCTTCATGTGTTCACCATAACCCTGCAAGAGCACTTGGTGATCTTACTGGTATCAAAAAGCACTTTTGTAGAAAGCATGGTGAGAAAAAGTGGAAATGTGATAAGTGTTCTAAGAAATATGCTGTTCAATCTGATTGGAAAGCTCATTCCAAAATATGTGGTACAAGGGAATACAAATGTGACTGTGGAACCATCTTTTCCAGAAGAGACAGCTTCATTACCCACAGAGCATTCTGTGACGCATTAGCTGAAGAGAACAACAAAGCAAACGAAGGAGTACTATCAAATTTGCAACACCAACCAATCTCAAATCTCGTTTCCTCATTACCTTTAAACCCCATTATTAACCCTCAAATTGGTGGAACTGTATCAGAATTCAACAACCATTCAGATCACAAGCTTCCATTATCATCACCTCATGAACTCATGTCAGTAGTGCCACCAAAACCCTTCAACAACAACATATTCACTAGAAGCCTTTCATCATCAACTTCATCTCCTTCTCTTCAACTCAGTTCAAATAATTCACTCAACAACATCTTGGAAGAAAATGGATCACTCCATTTATCTGCTGCAACTTCACCTCACATGTCAGCCACTGCATTGCTACAGAAAGCTGCTCAAATGGGTGCAACTGTGAGTAATAATAATGCAGGTATCATGACGGATAAAACTACCGTTGCTACAAACATGATGGCGCCGCCGCAGTTTGGTGTAGTGCAACAACAAGGACATGCTTTCATGAACCACTACATGCAACAGCAACAACAACACTACAATAATAATTTCAATGCAAATGGGATAATAAATGGAGGAGGCGTTGGAATGAATGGAGTTGATATGTTTAATGCTATATTGGATCAAAGTAAGGCTTTATCGAAGATCATTGAACAAAACAATCAAACTCAAAGCATTAATAGTGTTGTTGGAGGTGGAGGAGGAGGTGCATCAAGTAATAGTAATAATGTTATGAACATTAGTGGAAGTAAGGGTGGTGGAGATGTAATGACACTGGATTTGTTGGGGATAGGAGGAGGAGGTGCACATAGTAATTTCTATGGAGGGGGTACACAACAACAACAGGCAGAAAGTGCAGCAGCAGCAGCAGCTGATGAGGTTTGGAGAAATTGGTCAACCAAGAATGGAGGATTTGAATCTTTTTCAGCAACAAGCAACATTTGA
Protein sequence

>Msa1405050

MSNITSCDSGSFSTENTREDAVKQQQTDQILGQFHSPHSHTSTNNNNNSNGSNTDLQPPLKKKRNLPGNPDPSAEVIALSPTTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRTSAEIRKRVYVCPEPSCVHHNPARALGDLTGIKKHFCRKHGEKKWKCDKCSKKYAVQSDWKAHSKICGTREYKCDCGTIFSRRDSFITHRAFCDALAEENNKANEGVLSNLQHQPISNLVSSLPLNPIINPQIGGTVSEFNNHSDHKLPLSSPHELMSVVPPKPFNNNIFTRSLSSSTSSPSLQLSSNNSLNNILEENGSLHLSAATSPHMSATALLQKAAQMGATVSNNNAGIMTDKTTVATNMMAPPQFGVVQQQGHAFMNHYMQQQQQHYNNNFNANGIINGGGVGMNGVDMFNAILDQSKALSKIIEQNNQTQSINSVVGGGGGGASSNSNNVMNISGSKGGGDVMTLDLLGIGGGGAHSNFYGGGTQQQQAESAAAAAADEVWRNWSTKNGGFESFSATSNI*