AlfalfaGEDB | Msa0179160

M. sativa cultivar zhongmu-4 / Msa0179160

KEGG: K02980|K20308
GO: GO:0000184 GO:0000956 GO:0002181 GO:0003674 GO:0003735 GO:0005198 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012505 GO:0015031 GO:0015833 GO:0015935 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0019538 GO:0022626 GO:0022627 GO:0030867 GO:0031090 GO:0031974 GO:0031981 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0042175 GO:0042788 GO:0042886 GO:0042981 GO:0043043 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046872 GO:0046907 GO:0046914 GO:0048518 GO:0048519 GO:0048522 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1990904
PFAM: PF00253.24

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0179160	XP_023772684.2	100.000	56	0	0	1	56	31	86	1.46e-34	122

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0179160	sp\|Q680P8\|RS29_ARATH	92.857	56	4	0	1	56	1	56	6.73e-33	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0179160	A0A6S7LBD4	96.429	56	2	0	1	56	88	143	3.48e-34	122

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0136220	Msa0179160	0.807511	4.877769e-50	-8.615850e-47
Msa0179160	Msa0373350	0.841336	5.054393e-58	-8.615850e-47
Msa0179160	Msa0388520	0.804590	2.007911e-49	-8.615850e-47
Msa0179160	Msa1130680	0.808556	2.922220e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
Msa0179160	MtrunA17_Chr3g0143891	100.000	56	1	56	1	56	7.63e-38	119
Msa0179160	MtrunA17_Chr5g0393701	100.000	56	1	56	1	56	7.63e-38	119
Msa0179160	MtrunA17_Chr1g0207501	100.000	56	1	56	1	56	7.63e-38	119

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0179160	AT3G44010.1	92.857	56	4	1	56	1	56	6.84e-34	109
Msa0179160	AT3G44010.2	92.857	56	4	1	56	1	56	6.84e-34	109
Msa0179160	AT3G43980.1	92.857	56	4	1	56	1	56	6.84e-34	109
Msa0179160	AT4G33865.1	92.857	56	4	1	56	1	56	6.84e-34	109

Find 56 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTCTGTTTCTTTGTTCTTT+TGG	0.118055	1_4:-66590611	None:intergenic
TTATACAGAGAATCAAAATT+AGG	0.181591	1_4:-66590636	None:intergenic
TTGTCAGAATTTATGAATTA+AGG	0.214442	1_4:+66591705	Msa0179160:three_prime_UTR
CACAGCAATGCCAAGGAAAT+TGG	0.216340	1_4:+66591460	Msa0179160:CDS
ATACTATTAATGTTTGAATT+AGG	0.219534	1_4:+66591791	Msa0179160:three_prime_UTR
CAAGGAAATTGGCTTCATTA+AGG	0.276234	1_4:+66591471	Msa0179160:CDS
TATACAGAGAATCAAAATTA+GGG	0.302066	1_4:-66590635	None:intergenic
AGCAAGGAAGTCATAGTAAA+AGG	0.306874	1_4:-66591756	None:intergenic
TTCTTTGCCCAGCCAATCTT+AGG	0.336932	1_4:+66591596	Msa0179160:three_prime_UTR
GCCCAGCCAATCTTAGGTTT+CGG	0.337680	1_4:+66591602	Msa0179160:three_prime_UTR
CATGGATTGATAAGGAAGTA+TGG	0.378864	1_4:+66591412	Msa0179160:CDS
CCATGGGACACTCAAATGTT+TGG	0.382308	1_4:+66590751	Msa0179160:exon
TAAGGCCATGGTATACAGAT+TGG	0.383167	1_4:+66591859	Msa0179160:three_prime_UTR
CTATACTGAGATTCTATTGT+TGG	0.405937	1_4:+66592068	Msa0179160:three_prime_UTR
GAACCAGGACCATAGGTCTT+GGG	0.410602	1_4:-66590783	None:intergenic
TGCCGAAACCTAAGATTGGC+TGG	0.421303	1_4:-66591604	None:intergenic
CTTAATGAAGCCAATTTCCT+TGG	0.421882	1_4:-66591470	None:intergenic
AAGGCCATGGTATACAGATT+GGG	0.453002	1_4:+66591860	Msa0179160:three_prime_UTR
AGCATTGCCTGCAGCACATA+AGG	0.453112	1_4:-66591435	None:intergenic
GGAACCAGGACCATAGGTCT+TGG	0.464100	1_4:-66590784	None:intergenic
ATGGTCCTGGTTCCCGCACT+TGG	0.475887	1_4:+66590793	Msa0179160:CDS
AGACTCAAATGCTCTGCAGC+TGG	0.485140	1_4:-66590706	None:intergenic
CACCCCAAGACCTATGGTCC+TGG	0.491604	1_4:+66590780	Msa0179160:CDS
TTCTTAAGTTTCTTCAACAT+AGG	0.492218	1_4:-66591661	None:intergenic
GCAGTCACAAACTACATTGT+CGG	0.501456	1_4:-66592034	None:intergenic
AACTCTCACCCCAAGACCTA+TGG	0.505813	1_4:+66590774	Msa0179160:CDS
CTAATGCCGAAACCTAAGAT+TGG	0.515463	1_4:-66591608	None:intergenic
GGAATTCACATGGATTGATA+AGG	0.515737	1_4:+66591404	Msa0179160:CDS
ATATACCAAGTGCGGGAACC+AGG	0.531737	1_4:-66590798	None:intergenic
AGTATGGCCTTATGTGCTGC+AGG	0.540472	1_4:+66591428	Msa0179160:CDS
CCAAACATTTGAGTGTCCCA+TGG	0.543494	1_4:-66590751	None:intergenic
TGGTTTGTTGATTTGATCGC+CGG	0.550088	1_4:-66590731	None:intergenic
TGGCCTTAAAATTGCTGTGT+GGG	0.563277	1_4:-66591844	None:intergenic
GGCATTAGAGTGTTGTGAGG+TGG	0.565017	1_4:+66591623	Msa0179160:three_prime_UTR
TAACTTGTAGCCGTGTGTGC+GGG	0.573381	1_4:+66591383	Msa0179160:intron
GCGATCAAATCAACAAACCA+TGG	0.573789	1_4:+66590734	Msa0179160:exon
CATGTGAATTCCCGCACACA+CGG	0.574250	1_4:-66591393	None:intergenic
AAGAATGAATATACCAAGTG+CGG	0.579658	1_4:-66590806	None:intergenic
AGTGCGGGAACCAGGACCAT+AGG	0.585453	1_4:-66590790	None:intergenic
GCCGAAACCTAAGATTGGCT+GGG	0.586364	1_4:-66591603	None:intergenic
GCAGAGCATTTGAGTCTCAC+CGG	0.586976	1_4:+66590712	Msa0179160:five_prime_UTR
CAATTTCCTTGGCATTGCTG+TGG	0.593734	1_4:-66591459	None:intergenic
AACCAGGACCATAGGTCTTG+GGG	0.597207	1_4:-66590782	None:intergenic
CGTGTGTGCGGGAATTCACA+TGG	0.601988	1_4:+66591394	Msa0179160:CDS
AGAATGAATATACCAAGTGC+GGG	0.603783	1_4:-66590805	None:intergenic
CAGCTGGAAAATTGAGTGGA+TGG	0.611569	1_4:-66590690	None:intergenic
CAAAGAAAATAGCTCTTCAA+CGG	0.615426	1_4:-66591580	None:intergenic
CGATCAAATCAACAAACCAT+GGG	0.616523	1_4:+66590735	Msa0179160:exon
ATGCTTCCACAGCAATGCCA+AGG	0.620494	1_4:+66591453	Msa0179160:CDS
TTCGGCATTAGAGTGTTGTG+AGG	0.626041	1_4:+66591620	Msa0179160:three_prime_UTR
TCTGCAGCTGGAAAATTGAG+TGG	0.660954	1_4:-66590694	None:intergenic
ATAACTTGTAGCCGTGTGTG+CGG	0.665136	1_4:+66591382	Msa0179160:intron
CAAACCCAATCTGTATACCA+TGG	0.668308	1_4:-66591864	None:intergenic
GAAAATTGAGTGGATGGTGA+AGG	0.668610	1_4:-66590684	None:intergenic
GGCCTTAAAATTGCTGTGTG+GGG	0.685788	1_4:-66591843	None:intergenic
ATGGCCTTAAAATTGCTGTG+TGG	0.697221	1_4:-66591845	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGTTATTGATAATATGTTTT+GGG	+	chr1_4:66591022-66591041	Msa0179160:intron	15.0%
!!!	TTATGTTATTGCTATTATAT+CGG	+	chr1_4:66591209-66591228	Msa0179160:intron	15.0%
!!	AGGAAATGTATATATAAAAA+AGG	-	chr1_4:66591739-66591758	None:intergenic	15.0%
!!	ATACTATTAATGTTTGAATT+AGG	+	chr1_4:66591791-66591810	Msa0179160:three_prime_UTR	15.0%
!!!	TGTTATTGATAATATGTTTT+GGG	+	chr1_4:66591022-66591041	Msa0179160:intron	15.0%
!!!	TTATGTTATTGCTATTATAT+CGG	+	chr1_4:66591209-66591228	Msa0179160:intron	15.0%
!!	AGGAAATGTATATATAAAAA+AGG	-	chr1_4:66591739-66591758	None:intergenic	15.0%
!!	ATACTATTAATGTTTGAATT+AGG	+	chr1_4:66591791-66591810	Msa0179160:three_prime_UTR	15.0%
!!	TATACAGAGAATCAAAATTA+GGG	-	chr1_4:66590638-66590657	None:intergenic	20.0%
!!	TTATACAGAGAATCAAAATT+AGG	-	chr1_4:66590639-66590658	None:intergenic	20.0%
!!!	TTTTGATTCTCTGTATAAAT+TGG	+	chr1_4:66590641-66590660	Msa0179160:five_prime_UTR	20.0%
!!!	TAACCTTTTTCAATTCATTT+AGG	+	chr1_4:66590845-66590864	Msa0179160:intron	20.0%
!!	AAACCTAAATGAATTGAAAA+AGG	-	chr1_4:66590851-66590870	None:intergenic	20.0%
!!!	ATTTAGGTTTCTAGTATTTT+GGG	+	chr1_4:66590861-66590880	Msa0179160:intron	20.0%
!!	GATTCAAAATTCATAAAAGA+GGG	-	chr1_4:66590921-66590940	None:intergenic	20.0%
!!!	GTGTTATTGATAATATGTTT+TGG	+	chr1_4:66591021-66591040	Msa0179160:intron	20.0%
!!!	GTTATTGATAATATGTTTTG+GGG	+	chr1_4:66591023-66591042	Msa0179160:intron	20.0%
!!!	TTTGGGGATTTTTATTTTTT+TGG	+	chr1_4:66591039-66591058	Msa0179160:intron	20.0%
!!!	TTGGGGATTTTTATTTTTTT+GGG	+	chr1_4:66591040-66591059	Msa0179160:intron	20.0%
!!	TAATTAAGATTTGATTCAGA+TGG	+	chr1_4:66591104-66591123	Msa0179160:intron	20.0%
!!	GATATAATAGCAATAACATA+AGG	-	chr1_4:66591210-66591229	None:intergenic	20.0%
!!!	GATCAGTTGTTTTTTAATTT+AGG	+	chr1_4:66591328-66591347	Msa0179160:intron	20.0%
!!	GCAACAAAAATAAAAACAAA+AGG	-	chr1_4:66591556-66591575	None:intergenic	20.0%
!!	TTGTCAGAATTTATGAATTA+AGG	+	chr1_4:66591705-66591724	Msa0179160:three_prime_UTR	20.0%
!!	TATACAGAGAATCAAAATTA+GGG	-	chr1_4:66590638-66590657	None:intergenic	20.0%
!!	TTATACAGAGAATCAAAATT+AGG	-	chr1_4:66590639-66590658	None:intergenic	20.0%
!!!	TTTTGATTCTCTGTATAAAT+TGG	+	chr1_4:66590641-66590660	Msa0179160:five_prime_UTR	20.0%
!!!	TAACCTTTTTCAATTCATTT+AGG	+	chr1_4:66590845-66590864	Msa0179160:intron	20.0%
!!	AAACCTAAATGAATTGAAAA+AGG	-	chr1_4:66590851-66590870	None:intergenic	20.0%
!!!	ATTTAGGTTTCTAGTATTTT+GGG	+	chr1_4:66590861-66590880	Msa0179160:intron	20.0%
!!	GATTCAAAATTCATAAAAGA+GGG	-	chr1_4:66590921-66590940	None:intergenic	20.0%
!!!	GTGTTATTGATAATATGTTT+TGG	+	chr1_4:66591021-66591040	Msa0179160:intron	20.0%
!!!	GTTATTGATAATATGTTTTG+GGG	+	chr1_4:66591023-66591042	Msa0179160:intron	20.0%
!!!	TTTGGGGATTTTTATTTTTT+TGG	+	chr1_4:66591039-66591058	Msa0179160:intron	20.0%
!!!	TTGGGGATTTTTATTTTTTT+GGG	+	chr1_4:66591040-66591059	Msa0179160:intron	20.0%
!!	TAATTAAGATTTGATTCAGA+TGG	+	chr1_4:66591104-66591123	Msa0179160:intron	20.0%
!!	GATATAATAGCAATAACATA+AGG	-	chr1_4:66591210-66591229	None:intergenic	20.0%
!!!	GATCAGTTGTTTTTTAATTT+AGG	+	chr1_4:66591328-66591347	Msa0179160:intron	20.0%
!!	GCAACAAAAATAAAAACAAA+AGG	-	chr1_4:66591556-66591575	None:intergenic	20.0%
!!	TTGTCAGAATTTATGAATTA+AGG	+	chr1_4:66591705-66591724	Msa0179160:three_prime_UTR	20.0%
!!!	TTCTGTTTCTTTGTTCTTTT+GGG	-	chr1_4:66590613-66590632	None:intergenic	25.0%
!!!	TTTCTGTTTCTTTGTTCTTT+TGG	-	chr1_4:66590614-66590633	None:intergenic	25.0%
!!!	CATTTAGGTTTCTAGTATTT+TGG	+	chr1_4:66590860-66590879	Msa0179160:intron	25.0%
!	CAAAATGTAAACTTTAACAG+CGG	-	chr1_4:66590896-66590915	None:intergenic	25.0%
!	CGATTCAAAATTCATAAAAG+AGG	-	chr1_4:66590922-66590941	None:intergenic	25.0%
!!	TAATGGTTTTCTATTGCTTT+GGG	+	chr1_4:66590967-66590986	Msa0179160:intron	25.0%
!!	ATTTTACATGATTGTATCTG+AGG	-	chr1_4:66591517-66591536	None:intergenic	25.0%
!	TTCTTAAGTTTCTTCAACAT+AGG	-	chr1_4:66591664-66591683	None:intergenic	25.0%
!	GTATATCACAAAAAATAGCA+AGG	-	chr1_4:66591775-66591794	None:intergenic	25.0%
!!!	ATCAAAACATGCTGTTTTTT+CGG	+	chr1_4:66591984-66592003	Msa0179160:three_prime_UTR	25.0%
!!!	TTCTGTTTCTTTGTTCTTTT+GGG	-	chr1_4:66590613-66590632	None:intergenic	25.0%
!!!	TTTCTGTTTCTTTGTTCTTT+TGG	-	chr1_4:66590614-66590633	None:intergenic	25.0%
!!!	CATTTAGGTTTCTAGTATTT+TGG	+	chr1_4:66590860-66590879	Msa0179160:intron	25.0%
!	CAAAATGTAAACTTTAACAG+CGG	-	chr1_4:66590896-66590915	None:intergenic	25.0%
!	CGATTCAAAATTCATAAAAG+AGG	-	chr1_4:66590922-66590941	None:intergenic	25.0%
!!	TAATGGTTTTCTATTGCTTT+GGG	+	chr1_4:66590967-66590986	Msa0179160:intron	25.0%
!!	ATTTTACATGATTGTATCTG+AGG	-	chr1_4:66591517-66591536	None:intergenic	25.0%
!	TTCTTAAGTTTCTTCAACAT+AGG	-	chr1_4:66591664-66591683	None:intergenic	25.0%
!	GTATATCACAAAAAATAGCA+AGG	-	chr1_4:66591775-66591794	None:intergenic	25.0%
!!!	ATCAAAACATGCTGTTTTTT+CGG	+	chr1_4:66591984-66592003	Msa0179160:three_prime_UTR	25.0%
	AAGAATGAATATACCAAGTG+CGG	-	chr1_4:66590809-66590828	None:intergenic	30.0%
!!	GTGTTGTTTCTATGTTCTAA+TGG	+	chr1_4:66590950-66590969	Msa0179160:intron	30.0%
!	CTAATGGTTTTCTATTGCTT+TGG	+	chr1_4:66590966-66590985	Msa0179160:intron	30.0%
	ATAACATCAGCTGATATCAA+AGG	-	chr1_4:66591069-66591088	None:intergenic	30.0%
	TCTGAACATATGTAGTTTAG+TGG	+	chr1_4:66591180-66591199	Msa0179160:intron	30.0%
	CAAAGAAAATAGCTCTTCAA+CGG	-	chr1_4:66591583-66591602	None:intergenic	30.0%
	AAGAATGAATATACCAAGTG+CGG	-	chr1_4:66590809-66590828	None:intergenic	30.0%
!!	GTGTTGTTTCTATGTTCTAA+TGG	+	chr1_4:66590950-66590969	Msa0179160:intron	30.0%
!	CTAATGGTTTTCTATTGCTT+TGG	+	chr1_4:66590966-66590985	Msa0179160:intron	30.0%
	ATAACATCAGCTGATATCAA+AGG	-	chr1_4:66591069-66591088	None:intergenic	30.0%
	TCTGAACATATGTAGTTTAG+TGG	+	chr1_4:66591180-66591199	Msa0179160:intron	30.0%
	CAAAGAAAATAGCTCTTCAA+CGG	-	chr1_4:66591583-66591602	None:intergenic	30.0%
!	TGTTTCTTTGTTCTTTTGGG+TGG	-	chr1_4:66590610-66590629	None:intergenic	35.0%
	CGATCAAATCAACAAACCAT+GGG	+	chr1_4:66590735-66590754	Msa0179160:exon	35.0%
	AGAATGAATATACCAAGTGC+GGG	-	chr1_4:66590808-66590827	None:intergenic	35.0%
	TGCTATTATATCGGTACTAG+AGG	+	chr1_4:66591218-66591237	Msa0179160:intron	35.0%
!	GGAATTCACATGGATTGATA+AGG	+	chr1_4:66591404-66591423	Msa0179160:CDS	35.0%
!	CATGGATTGATAAGGAAGTA+TGG	+	chr1_4:66591412-66591431	Msa0179160:CDS	35.0%
	CTTAATGAAGCCAATTTCCT+TGG	-	chr1_4:66591473-66591492	None:intergenic	35.0%
!	CAAGGAAATTGGCTTCATTA+AGG	+	chr1_4:66591471-66591490	Msa0179160:CDS	35.0%
	AGCAAGGAAGTCATAGTAAA+AGG	-	chr1_4:66591759-66591778	None:intergenic	35.0%
!	TGTTTCTTTGTTCTTTTGGG+TGG	-	chr1_4:66590610-66590629	None:intergenic	35.0%
	CGATCAAATCAACAAACCAT+GGG	+	chr1_4:66590735-66590754	Msa0179160:exon	35.0%
	AGAATGAATATACCAAGTGC+GGG	-	chr1_4:66590808-66590827	None:intergenic	35.0%
	TGCTATTATATCGGTACTAG+AGG	+	chr1_4:66591218-66591237	Msa0179160:intron	35.0%
!	GGAATTCACATGGATTGATA+AGG	+	chr1_4:66591404-66591423	Msa0179160:CDS	35.0%
!	CATGGATTGATAAGGAAGTA+TGG	+	chr1_4:66591412-66591431	Msa0179160:CDS	35.0%
	CTTAATGAAGCCAATTTCCT+TGG	-	chr1_4:66591473-66591492	None:intergenic	35.0%
!	CAAGGAAATTGGCTTCATTA+AGG	+	chr1_4:66591471-66591490	Msa0179160:CDS	35.0%
	AGCAAGGAAGTCATAGTAAA+AGG	-	chr1_4:66591759-66591778	None:intergenic	35.0%
!!	TTCTTTGTTCTTTTGGGTGG+TGG	-	chr1_4:66590607-66590626	None:intergenic	40.0%
	GAAAATTGAGTGGATGGTGA+AGG	-	chr1_4:66590687-66590706	None:intergenic	40.0%
!!	TGGTTTGTTGATTTGATCGC+CGG	-	chr1_4:66590734-66590753	None:intergenic	40.0%
	GCGATCAAATCAACAAACCA+TGG	+	chr1_4:66590734-66590753	Msa0179160:exon	40.0%
!!	TGCTTTGGGTTGTACTGTTA+TGG	+	chr1_4:66590981-66591000	Msa0179160:intron	40.0%
	CTAATGCCGAAACCTAAGAT+TGG	-	chr1_4:66591611-66591630	None:intergenic	40.0%
!	TGGCCTTAAAATTGCTGTGT+GGG	-	chr1_4:66591847-66591866	None:intergenic	40.0%
!	ATGGCCTTAAAATTGCTGTG+TGG	-	chr1_4:66591848-66591867	None:intergenic	40.0%
!!!	ACACAGCAATTTTAAGGCCA+TGG	+	chr1_4:66591847-66591866	Msa0179160:three_prime_UTR	40.0%
	TAAGGCCATGGTATACAGAT+TGG	+	chr1_4:66591859-66591878	Msa0179160:three_prime_UTR	40.0%
	AAGGCCATGGTATACAGATT+GGG	+	chr1_4:66591860-66591879	Msa0179160:three_prime_UTR	40.0%
	CAAACCCAATCTGTATACCA+TGG	-	chr1_4:66591867-66591886	None:intergenic	40.0%
	GCAGTCACAAACTACATTGT+CGG	-	chr1_4:66592037-66592056	None:intergenic	40.0%
!!	TTCTTTGTTCTTTTGGGTGG+TGG	-	chr1_4:66590607-66590626	None:intergenic	40.0%
	GAAAATTGAGTGGATGGTGA+AGG	-	chr1_4:66590687-66590706	None:intergenic	40.0%
!!	TGGTTTGTTGATTTGATCGC+CGG	-	chr1_4:66590734-66590753	None:intergenic	40.0%
	GCGATCAAATCAACAAACCA+TGG	+	chr1_4:66590734-66590753	Msa0179160:exon	40.0%
!!	TGCTTTGGGTTGTACTGTTA+TGG	+	chr1_4:66590981-66591000	Msa0179160:intron	40.0%
	CTAATGCCGAAACCTAAGAT+TGG	-	chr1_4:66591611-66591630	None:intergenic	40.0%
!	TGGCCTTAAAATTGCTGTGT+GGG	-	chr1_4:66591847-66591866	None:intergenic	40.0%
!	ATGGCCTTAAAATTGCTGTG+TGG	-	chr1_4:66591848-66591867	None:intergenic	40.0%
!!!	ACACAGCAATTTTAAGGCCA+TGG	+	chr1_4:66591847-66591866	Msa0179160:three_prime_UTR	40.0%
	TAAGGCCATGGTATACAGAT+TGG	+	chr1_4:66591859-66591878	Msa0179160:three_prime_UTR	40.0%
	AAGGCCATGGTATACAGATT+GGG	+	chr1_4:66591860-66591879	Msa0179160:three_prime_UTR	40.0%
	CAAACCCAATCTGTATACCA+TGG	-	chr1_4:66591867-66591886	None:intergenic	40.0%
	GCAGTCACAAACTACATTGT+CGG	-	chr1_4:66592037-66592056	None:intergenic	40.0%
	CAGCTGGAAAATTGAGTGGA+TGG	-	chr1_4:66590693-66590712	None:intergenic	45.0%
	TCTGCAGCTGGAAAATTGAG+TGG	-	chr1_4:66590697-66590716	None:intergenic	45.0%
	CCAAACATTTGAGTGTCCCA+TGG	-	chr1_4:66590754-66590773	None:intergenic	45.0%
	CCATGGGACACTCAAATGTT+TGG	+	chr1_4:66590751-66590770	Msa0179160:exon	45.0%
!	ATAACTTGTAGCCGTGTGTG+CGG	+	chr1_4:66591382-66591401	Msa0179160:intron	45.0%
	CAATTTCCTTGGCATTGCTG+TGG	-	chr1_4:66591462-66591481	None:intergenic	45.0%
	CACAGCAATGCCAAGGAAAT+TGG	+	chr1_4:66591460-66591479	Msa0179160:CDS	45.0%
	TTCTTTGCCCAGCCAATCTT+AGG	+	chr1_4:66591596-66591615	Msa0179160:three_prime_UTR	45.0%
!!	TTCGGCATTAGAGTGTTGTG+AGG	+	chr1_4:66591620-66591639	Msa0179160:three_prime_UTR	45.0%
!	TGCCCCACACAGCAATTTTA+AGG	+	chr1_4:66591841-66591860	Msa0179160:three_prime_UTR	45.0%
	GGCCTTAAAATTGCTGTGTG+GGG	-	chr1_4:66591846-66591865	None:intergenic	45.0%
	CAGCTGGAAAATTGAGTGGA+TGG	-	chr1_4:66590693-66590712	None:intergenic	45.0%
	TCTGCAGCTGGAAAATTGAG+TGG	-	chr1_4:66590697-66590716	None:intergenic	45.0%
	CCAAACATTTGAGTGTCCCA+TGG	-	chr1_4:66590754-66590773	None:intergenic	45.0%
	CCATGGGACACTCAAATGTT+TGG	+	chr1_4:66590751-66590770	Msa0179160:exon	45.0%
!	ATAACTTGTAGCCGTGTGTG+CGG	+	chr1_4:66591382-66591401	Msa0179160:intron	45.0%
	CAATTTCCTTGGCATTGCTG+TGG	-	chr1_4:66591462-66591481	None:intergenic	45.0%
	CACAGCAATGCCAAGGAAAT+TGG	+	chr1_4:66591460-66591479	Msa0179160:CDS	45.0%
	TTCTTTGCCCAGCCAATCTT+AGG	+	chr1_4:66591596-66591615	Msa0179160:three_prime_UTR	45.0%
!!	TTCGGCATTAGAGTGTTGTG+AGG	+	chr1_4:66591620-66591639	Msa0179160:three_prime_UTR	45.0%
!	TGCCCCACACAGCAATTTTA+AGG	+	chr1_4:66591841-66591860	Msa0179160:three_prime_UTR	45.0%
	GGCCTTAAAATTGCTGTGTG+GGG	-	chr1_4:66591846-66591865	None:intergenic	45.0%
	AGACTCAAATGCTCTGCAGC+TGG	-	chr1_4:66590709-66590728	None:intergenic	50.0%
!	GCAGAGCATTTGAGTCTCAC+CGG	+	chr1_4:66590712-66590731	Msa0179160:five_prime_UTR	50.0%
	AACTCTCACCCCAAGACCTA+TGG	+	chr1_4:66590774-66590793	Msa0179160:CDS	50.0%
	AACCAGGACCATAGGTCTTG+GGG	-	chr1_4:66590785-66590804	None:intergenic	50.0%
	GAACCAGGACCATAGGTCTT+GGG	-	chr1_4:66590786-66590805	None:intergenic	50.0%
	ATATACCAAGTGCGGGAACC+AGG	-	chr1_4:66590801-66590820	None:intergenic	50.0%
!	TAACTTGTAGCCGTGTGTGC+GGG	+	chr1_4:66591383-66591402	Msa0179160:intron	50.0%
	CATGTGAATTCCCGCACACA+CGG	-	chr1_4:66591396-66591415	None:intergenic	50.0%
!!	AGTATGGCCTTATGTGCTGC+AGG	+	chr1_4:66591428-66591447	Msa0179160:CDS	50.0%
	AGCATTGCCTGCAGCACATA+AGG	-	chr1_4:66591438-66591457	None:intergenic	50.0%
	ATGCTTCCACAGCAATGCCA+AGG	+	chr1_4:66591453-66591472	Msa0179160:CDS	50.0%
	GCCCAGCCAATCTTAGGTTT+CGG	+	chr1_4:66591602-66591621	Msa0179160:three_prime_UTR	50.0%
!	GCCGAAACCTAAGATTGGCT+GGG	-	chr1_4:66591606-66591625	None:intergenic	50.0%
	TGCCGAAACCTAAGATTGGC+TGG	-	chr1_4:66591607-66591626	None:intergenic	50.0%
!!	GGCATTAGAGTGTTGTGAGG+TGG	+	chr1_4:66591623-66591642	Msa0179160:three_prime_UTR	50.0%
	AGACTCAAATGCTCTGCAGC+TGG	-	chr1_4:66590709-66590728	None:intergenic	50.0%
!	GCAGAGCATTTGAGTCTCAC+CGG	+	chr1_4:66590712-66590731	Msa0179160:five_prime_UTR	50.0%
	AACTCTCACCCCAAGACCTA+TGG	+	chr1_4:66590774-66590793	Msa0179160:CDS	50.0%
	AACCAGGACCATAGGTCTTG+GGG	-	chr1_4:66590785-66590804	None:intergenic	50.0%
	GAACCAGGACCATAGGTCTT+GGG	-	chr1_4:66590786-66590805	None:intergenic	50.0%
	ATATACCAAGTGCGGGAACC+AGG	-	chr1_4:66590801-66590820	None:intergenic	50.0%
!	TAACTTGTAGCCGTGTGTGC+GGG	+	chr1_4:66591383-66591402	Msa0179160:intron	50.0%
	CATGTGAATTCCCGCACACA+CGG	-	chr1_4:66591396-66591415	None:intergenic	50.0%
!!	AGTATGGCCTTATGTGCTGC+AGG	+	chr1_4:66591428-66591447	Msa0179160:CDS	50.0%
	AGCATTGCCTGCAGCACATA+AGG	-	chr1_4:66591438-66591457	None:intergenic	50.0%
	ATGCTTCCACAGCAATGCCA+AGG	+	chr1_4:66591453-66591472	Msa0179160:CDS	50.0%
	GCCCAGCCAATCTTAGGTTT+CGG	+	chr1_4:66591602-66591621	Msa0179160:three_prime_UTR	50.0%
!	GCCGAAACCTAAGATTGGCT+GGG	-	chr1_4:66591606-66591625	None:intergenic	50.0%
	TGCCGAAACCTAAGATTGGC+TGG	-	chr1_4:66591607-66591626	None:intergenic	50.0%
!!	GGCATTAGAGTGTTGTGAGG+TGG	+	chr1_4:66591623-66591642	Msa0179160:three_prime_UTR	50.0%
	GGAACCAGGACCATAGGTCT+TGG	-	chr1_4:66590787-66590806	None:intergenic	55.0%
	CGTGTGTGCGGGAATTCACA+TGG	+	chr1_4:66591394-66591413	Msa0179160:CDS	55.0%
	GGAACCAGGACCATAGGTCT+TGG	-	chr1_4:66590787-66590806	None:intergenic	55.0%
	CGTGTGTGCGGGAATTCACA+TGG	+	chr1_4:66591394-66591413	Msa0179160:CDS	55.0%
	CACCCCAAGACCTATGGTCC+TGG	+	chr1_4:66590780-66590799	Msa0179160:CDS	60.0%
	AGTGCGGGAACCAGGACCAT+AGG	-	chr1_4:66590793-66590812	None:intergenic	60.0%
	ATGGTCCTGGTTCCCGCACT+TGG	+	chr1_4:66590793-66590812	Msa0179160:CDS	60.0%
	CACCCCAAGACCTATGGTCC+TGG	+	chr1_4:66590780-66590799	Msa0179160:CDS	60.0%
	AGTGCGGGAACCAGGACCAT+AGG	-	chr1_4:66590793-66590812	None:intergenic	60.0%
	ATGGTCCTGGTTCCCGCACT+TGG	+	chr1_4:66590793-66590812	Msa0179160:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	66590600	66592089	66590600	ID=Msa0179160;Name=Msa0179160
chr1_4	mRNA	66590600	66592089	66590600	ID=Msa0179160-mRNA-1;Parent=Msa0179160;Name=Msa0179160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=153\|1\|1\|1\|0.5\|0.66\|3\|503\|56
chr1_4	exon	66590600	66590814	66590600	ID=Msa0179160-mRNA-1:exon:21819;Parent=Msa0179160-mRNA-1
chr1_4	exon	66591393	66591492	66591393	ID=Msa0179160-mRNA-1:exon:21820;Parent=Msa0179160-mRNA-1
chr1_4	exon	66591578	66592089	66591578	ID=Msa0179160-mRNA-1:exon:21821;Parent=Msa0179160-mRNA-1
chr1_4	five_prime_UTR	66590600	66590752	66590600	ID=Msa0179160-mRNA-1:five_prime_utr;Parent=Msa0179160-mRNA-1
chr1_4	CDS	66590753	66590814	66590753	ID=Msa0179160-mRNA-1:cds;Parent=Msa0179160-mRNA-1
chr1_4	CDS	66591393	66591492	66591393	ID=Msa0179160-mRNA-1:cds;Parent=Msa0179160-mRNA-1
chr1_4	CDS	66591578	66591586	66591578	ID=Msa0179160-mRNA-1:cds;Parent=Msa0179160-mRNA-1
chr1_4	three_prime_UTR	66591587	66592089	66591587	ID=Msa0179160-mRNA-1:three_prime_utr;Parent=Msa0179160-mRNA-1

Gene Sequence	>Msa0179160 ATGGGACACTCAAATGTTTGGAACTCTCACCCCAAGACCTATGGTCCTGGTTCCCGCACTTGCCGTGTGTGCGGGAATTCACATGGATTGATAAGGAAGTATGGCCTTATGTGCTGCAGGCAATGCTTCCACAGCAATGCCAAGGAAATTGGCTTCATTAAGTACCGTTGA
Protein sequence	>Msa0179160 MGHSNVWNSHPKTYGPGSRTCRVCGNSHGLIRKYGLMCCRQCFHSNAKEIGFIKYR*