AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0183330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0183330 MtrunA17_Chr1g0211251 98.489 397 6 0 1 397 1 397 0.0 814
Msa0183330 MtrunA17_Chr7g0250061 81.395 387 72 0 6 392 1 387 0.0 643
Msa0183330 MtrunA17_Chr8g0371321 75.000 388 95 1 1 388 1 386 0.0 605
Msa0183330 MtrunA17_Chr1g0213351 68.852 366 106 3 12 377 13 370 0.0 519
Msa0183330 MtrunA17_Chr7g0263281 63.934 366 128 2 11 376 4 365 2.29e-166 470
Msa0183330 MtrunA17_Chr2g0294291 58.621 377 144 4 1 374 1 368 4.28e-161 457
Msa0183330 MtrunA17_Chr1g0181901 56.359 401 143 4 8 377 1 400 2.48e-154 441
Msa0183330 MtrunA17_Chr1g0175381 53.927 382 157 5 1 375 1 370 3.69e-149 427
Msa0183330 MtrunA17_Chr1g0207571 55.125 361 147 3 9 367 2 349 4.98e-143 411
Msa0183330 MtrunA17_Chr3g0089711 45.109 368 181 6 1 362 1 353 3.90e-103 309
Msa0183330 MtrunA17_Chr3g0089721 44.837 368 182 6 1 362 1 353 1.48e-102 308
Msa0183330 MtrunA17_Chr7g0256751 49.221 321 146 6 43 356 38 348 2.00e-97 297
Msa0183330 MtrunA17_Chr7g0256771 47.601 271 131 4 39 307 35 296 9.49e-81 250
Msa0183330 MtrunA17_Chr3g0089691 48.387 248 121 3 120 362 22 267 2.84e-78 244
Msa0183330 MtrunA17_Chr4g0063111 35.843 332 190 7 31 356 33 347 5.96e-60 202
Msa0183330 MtrunA17_Chr4g0063051 34.104 346 181 12 35 375 22 325 2.76e-50 172
Msa0183330 MtrunA17_Chr7g0238501 30.872 298 177 7 81 359 263 550 4.35e-42 155
Msa0183330 MtrunA17_Chr2g0295991 32.967 273 171 4 101 366 220 487 1.77e-40 149
Msa0183330 MtrunA17_Chr4g0039061 31.973 294 178 7 80 359 201 486 1.26e-39 147
Msa0183330 MtrunA17_Chr7g0256761 41.294 201 95 5 167 362 5 187 3.31e-39 139
Msa0183330 MtrunA17_Chr4g0067301 33.977 259 156 5 117 365 245 498 1.70e-38 144
Msa0183330 MtrunA17_Chr4g0031521 28.929 280 157 5 117 359 425 699 3.57e-32 128
Msa0183330 MtrunA17_Chr4g0054811 28.094 299 156 5 115 359 478 771 8.91e-32 127
Msa0183330 MtrunA17_Chr1g0193691 38.202 178 101 3 186 359 715 887 3.19e-29 120
Msa0183330 MtrunA17_Chr4g0062951 35.638 188 103 7 115 289 1 183 2.48e-27 107
Msa0183330 MtrunA17_Chr5g0430481 30.667 225 114 6 80 303 153 336 3.86e-25 105
Msa0183330 MtrunA17_Chr1g0212961 30.579 242 118 8 81 318 126 321 2.60e-24 102
Msa0183330 MtrunA17_Chr3g0105371 28.159 277 143 9 80 355 117 338 3.19e-24 102
Msa0183330 MtrunA17_Chr3g0105351 26.619 278 146 7 80 355 93 314 1.10e-23 100
Msa0183330 MtrunA17_Chr2g0317021 27.640 322 154 11 42 355 82 332 6.09e-23 98.6
Msa0183330 MtrunA17_Chr4g0050381 29.126 309 150 13 48 353 32 274 1.15e-20 91.3
Msa0183330 MtrunA17_Chr4g0051661 28.689 244 123 10 81 318 183 381 6.50e-20 90.9
Msa0183330 MtrunA17_Chr3g0115851 28.013 307 156 10 48 353 37 279 8.59e-20 88.6
Msa0183330 MtrunA17_Chr4g0070871 27.667 300 169 10 15 303 185 447 1.01e-19 90.9
Msa0183330 MtrunA17_Chr8g0360351 26.498 317 160 10 42 353 31 279 1.48e-19 88.2
Msa0183330 MtrunA17_Chr3g0126511 24.691 243 140 6 59 301 107 306 2.62e-19 88.6
Msa0183330 MtrunA17_Chr1g0150451 28.099 242 128 8 83 320 110 309 3.43e-19 87.8
Msa0183330 MtrunA17_Chr2g0279151 28.159 277 132 10 81 353 65 278 6.19e-19 86.3
Msa0183330 MtrunA17_Chr3g0136711 26.102 295 151 10 83 372 67 299 9.45e-19 85.9
Msa0183330 MtrunA17_Chr7g0223641 30.973 226 111 8 145 358 7 199 1.42e-18 83.6
Msa0183330 MtrunA17_Chr5g0426141 28.339 307 155 11 48 353 37 279 3.44e-18 84.0
Msa0183330 MtrunA17_Chr3g0135961 27.152 302 145 12 81 355 286 539 5.23e-18 85.9
Msa0183330 MtrunA17_Chr3g0138281 27.915 283 143 12 76 353 117 343 6.97e-18 84.7
Msa0183330 MtrunA17_Chr2g0293811 26.621 293 138 11 80 355 99 331 1.32e-17 83.6
Msa0183330 MtrunA17_Chr5g0407161 26.502 283 144 10 77 353 119 343 4.24e-17 82.4
Msa0183330 MtrunA17_Chr1g0158781 28.622 283 164 8 30 312 96 340 4.49e-17 82.4
Msa0183330 MtrunA17_Chr7g0272061 32.227 211 95 9 161 361 195 367 6.59e-17 81.6
Msa0183330 MtrunA17_Chr2g0299981 26.800 250 122 7 81 303 267 482 2.76e-16 80.5
Msa0183330 MtrunA17_Chr3g0112291 26.689 296 153 10 81 355 123 375 3.30e-16 79.7
Msa0183330 MtrunA17_Chr5g0435721 26.695 236 122 5 81 303 223 420 1.86e-15 77.8
Msa0183330 MtrunA17_Chr1g0194191 26.400 250 144 9 80 320 117 335 3.83e-15 76.6
Msa0183330 MtrunA17_Chr1g0150071 25.461 271 122 11 80 303 51 288 3.95e-15 76.3
Msa0183330 MtrunA17_Chr4g0065141 25.874 286 148 12 80 357 123 352 6.25e-15 75.9
Msa0183330 MtrunA17_Chr1g0171571 25.606 289 155 11 72 355 411 644 7.70e-14 73.2
Msa0183330 MtrunA17_Chr3g0144111 24.483 290 133 8 79 362 25 234 7.87e-14 71.2
Msa0183330 MtrunA17_Chr3g0136811 25.279 269 123 10 80 302 51 287 9.69e-14 72.0
Msa0183330 MtrunA17_Chr1g0150781 25.839 298 154 11 81 355 290 543 1.40e-13 72.4
Msa0183330 MtrunA17_Chr6g0483411 25.746 268 140 10 84 332 82 309 1.48e-13 71.2
Msa0183330 MtrunA17_Chr4g0001761 25.275 273 131 8 72 302 46 287 1.73e-13 71.2
Msa0183330 MtrunA17_Chr5g0396731 26.280 293 172 10 74 359 99 354 6.92e-13 69.3
Msa0183330 MtrunA17_Chr8g0342431 29.379 177 105 5 162 336 210 368 8.99e-13 69.3
Msa0183330 MtrunA17_Chr7g0221691 28.643 199 108 8 162 358 245 411 1.96e-12 68.6
Msa0183330 MtrunA17_Chr1g0192421 25.987 304 158 14 81 357 89 352 3.66e-12 67.0
Msa0183330 MtrunA17_Chr3g0127091 25.101 247 149 6 80 307 88 317 4.26e-12 67.0
Msa0183330 MtrunA17_Chr2g0295401 26.797 306 153 15 81 357 92 355 6.30e-12 66.6
Msa0183330 MtrunA17_Chr4g0004971 27.208 283 149 14 81 332 78 334 4.43e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0183330 AT4G38520.2 73.214 392 105 0 1 392 1 392 0.0 619
Msa0183330 AT4G38520.1 73.214 392 105 0 1 392 1 392 0.0 619
Msa0183330 AT5G66080.1 74.346 382 97 1 1 381 1 382 0.0 608
Msa0183330 AT3G51370.1 73.491 381 96 2 1 381 1 376 0.0 597
Msa0183330 AT3G51370.3 73.491 381 96 2 1 381 1 376 0.0 597
Msa0183330 AT3G12620.2 67.200 375 122 1 3 377 5 378 0.0 533
Msa0183330 AT3G12620.1 67.200 375 122 1 3 377 5 378 0.0 533
Msa0183330 AT3G55050.1 63.874 382 127 4 8 385 9 383 0.0 518
Msa0183330 AT3G55050.2 63.874 382 127 4 8 385 9 383 0.0 518
Msa0183330 AT5G66080.2 74.818 274 69 0 108 381 5 278 2.04e-161 455
Msa0183330 AT5G02760.1 61.247 369 137 4 8 376 1 363 4.13e-157 447
Msa0183330 AT3G51370.2 70.748 294 82 2 88 381 2 291 5.10e-157 444
Msa0183330 AT3G17090.1 55.703 377 142 5 1 363 1 366 7.02e-150 429
Msa0183330 AT4G33920.1 52.083 384 156 5 1 381 1 359 7.76e-142 409
Msa0183330 AT5G06750.3 55.780 346 139 4 34 375 43 378 2.58e-138 400
Msa0183330 AT5G06750.1 55.780 346 139 4 34 375 43 378 2.58e-138 400
Msa0183330 AT3G17090.2 52.761 326 129 5 1 312 1 315 1.62e-118 347
Msa0183330 AT4G33920.2 54.643 280 119 3 105 381 2 276 1.49e-110 326
Msa0183330 AT5G06750.2 52.805 303 131 5 77 375 2 296 5.11e-107 318
Msa0183330 AT4G33920.3 55.378 251 104 3 134 381 1 246 2.27e-100 299
Msa0183330 AT3G16560.1 30.341 323 181 8 81 365 175 491 6.92e-40 148
Msa0183330 AT3G16560.4 30.341 323 181 8 81 365 175 491 6.92e-40 148
Msa0183330 AT3G16560.2 30.341 323 181 8 81 365 175 491 6.92e-40 148
Msa0183330 AT5G02400.1 32.258 279 147 6 118 359 396 669 7.56e-38 145
Msa0183330 AT3G09400.1 32.609 276 143 8 118 359 379 645 1.49e-37 144
Msa0183330 AT1G07630.1 29.643 280 155 5 117 359 383 657 1.24e-35 138
Msa0183330 AT2G28890.1 29.930 284 154 6 116 359 371 649 1.30e-34 135
Msa0183330 AT2G35350.1 28.716 296 160 5 115 364 489 779 3.27e-34 135
Msa0183330 AT3G16560.3 30.208 288 157 8 81 330 175 456 6.71e-32 126
Msa0183330 AT1G07160.1 29.537 281 143 7 77 355 149 376 4.10e-29 117
Msa0183330 AT3G09400.2 31.818 242 122 8 118 325 379 611 3.49e-28 117
Msa0183330 AT2G40180.1 28.912 294 146 11 73 359 152 389 1.10e-27 113
Msa0183330 AT2G46920.3 35.393 178 106 3 186 359 667 839 3.22e-27 114
Msa0183330 AT2G46920.1 35.393 178 106 3 186 359 667 839 3.22e-27 114
Msa0183330 AT2G46920.2 35.393 178 106 3 186 359 667 839 3.22e-27 114
Msa0183330 AT2G46920.4 35.393 178 106 3 186 359 667 839 3.22e-27 114
Msa0183330 AT2G30020.1 28.727 275 140 8 83 355 172 392 8.96e-25 105
Msa0183330 AT1G67820.2 29.333 225 116 5 80 302 114 297 3.68e-23 100
Msa0183330 AT1G67820.1 29.333 225 116 5 80 302 150 333 4.38e-23 100
Msa0183330 AT4G28400.3 28.374 289 153 12 42 324 10 250 4.11e-21 92.4
Msa0183330 AT4G28400.1 28.374 289 153 12 42 324 32 272 7.10e-21 92.0
Msa0183330 AT2G20630.1 30.165 242 119 10 84 319 66 263 7.40e-21 92.0
Msa0183330 AT1G43900.1 29.433 282 133 10 75 353 148 366 1.00e-20 93.2
Msa0183330 AT4G31750.2 27.642 246 131 8 83 323 66 269 1.09e-20 92.0
Msa0183330 AT4G31750.1 27.642 246 131 7 83 323 66 269 1.12e-20 92.0
Msa0183330 AT4G31750.3 27.642 246 131 7 83 323 66 269 1.12e-20 92.0
Msa0183330 AT1G34750.2 26.923 338 173 12 19 355 17 281 1.31e-20 91.3
Msa0183330 AT1G34750.1 26.923 338 173 12 19 355 17 281 1.31e-20 91.3
Msa0183330 AT1G34750.3 26.923 338 173 12 19 355 17 281 1.31e-20 91.3
Msa0183330 AT2G20630.2 30.165 242 119 10 84 319 66 263 1.84e-20 91.3
Msa0183330 AT4G26080.1 26.829 287 162 9 81 355 172 422 2.34e-20 92.8
Msa0183330 AT5G53140.1 24.126 286 169 9 36 319 93 332 2.09e-19 89.7
Msa0183330 AT5G53140.2 24.126 286 169 9 36 319 93 332 2.09e-19 89.7
Msa0183330 AT5G10740.1 27.572 243 128 8 83 320 66 265 2.32e-19 89.0
Msa0183330 AT5G10740.2 27.572 243 128 8 83 320 66 265 2.32e-19 89.0
Msa0183330 AT1G22280.3 28.846 312 153 11 48 353 36 284 2.43e-19 87.8
Msa0183330 AT5G24940.1 27.347 245 130 9 81 320 64 265 6.73e-19 88.6
Msa0183330 AT1G78200.1 27.915 283 153 10 48 324 35 272 6.91e-19 86.7
Msa0183330 AT1G78200.3 27.915 283 153 10 48 324 35 272 6.91e-19 86.7
Msa0183330 AT1G78200.2 27.915 283 153 10 48 324 35 272 6.91e-19 86.7
Msa0183330 AT1G22280.1 29.355 310 148 12 48 353 36 278 8.38e-19 86.3
Msa0183330 AT2G25620.1 25.914 301 156 11 65 355 113 356 9.16e-19 87.8
Msa0183330 AT2G25620.2 25.914 301 156 11 65 355 113 356 9.16e-19 87.8
Msa0183330 AT2G33700.1 27.915 283 135 11 79 353 121 342 3.47e-18 85.9
Msa0183330 AT1G78200.5 27.574 272 146 10 59 324 1 227 4.81e-18 83.2
Msa0183330 AT1G78200.4 27.574 272 146 10 59 324 1 227 4.81e-18 83.2
Msa0183330 AT1G07430.1 28.270 237 118 6 81 303 157 355 6.63e-18 85.5
Msa0183330 AT3G15260.2 26.844 339 171 13 19 353 21 286 3.21e-17 82.0
Msa0183330 AT3G15260.1 26.844 339 171 13 19 353 21 286 3.21e-17 82.0
Msa0183330 AT3G51470.2 24.749 299 156 8 79 369 60 297 5.58e-17 81.6
Msa0183330 AT3G51470.1 24.749 299 156 8 79 369 104 341 5.80e-17 82.0
Msa0183330 AT5G57050.1 25.965 285 168 7 81 355 160 411 1.04e-16 81.6
Msa0183330 AT5G57050.2 25.965 285 168 7 81 355 120 371 1.30e-16 81.3
Msa0183330 AT4G08260.1 26.007 273 117 8 83 355 21 208 2.78e-16 77.8
Msa0183330 AT1G18030.1 27.626 257 133 9 83 320 111 333 3.73e-16 79.7
Msa0183330 AT3G16800.2 27.243 301 150 13 80 355 92 348 4.10e-16 79.3
Msa0183330 AT3G16800.1 27.243 301 150 13 80 355 92 348 4.10e-16 79.3
Msa0183330 AT3G16800.3 27.243 301 150 13 80 355 92 348 4.10e-16 79.3
Msa0183330 AT1G17550.1 26.646 319 156 13 72 355 226 501 4.71e-16 80.1
Msa0183330 AT1G17550.2 26.646 319 156 13 72 355 226 501 4.71e-16 80.1
Msa0183330 AT1G72770.4 26.710 307 154 10 79 355 236 501 5.30e-16 80.1
Msa0183330 AT1G72770.1 26.710 307 154 10 79 355 236 501 5.30e-16 80.1
Msa0183330 AT1G72770.3 26.710 307 154 10 79 355 236 501 5.30e-16 80.1
Msa0183330 AT4G28400.2 27.559 254 133 10 42 291 32 238 2.45e-15 75.9
Msa0183330 AT3G62260.3 27.692 260 145 10 71 319 17 244 2.71e-15 76.3
Msa0183330 AT3G05640.2 27.797 295 155 13 81 355 93 349 3.94e-15 76.6
Msa0183330 AT3G05640.1 27.797 295 155 13 81 355 93 349 3.94e-15 76.6
Msa0183330 AT3G05640.3 27.797 295 155 13 81 355 93 349 3.94e-15 76.6
Msa0183330 AT5G26010.1 27.027 296 161 11 78 355 66 324 4.70e-15 76.3
Msa0183330 AT5G59220.1 27.984 243 122 8 81 310 148 350 6.03e-15 76.3
Msa0183330 AT3G62260.1 27.692 260 145 10 71 319 106 333 8.98e-15 75.9
Msa0183330 AT3G62260.2 27.692 260 145 10 71 319 107 334 9.20e-15 75.9
Msa0183330 AT4G31860.3 25.926 270 120 11 80 302 51 287 1.07e-14 74.7
Msa0183330 AT3G16800.4 29.515 227 110 9 141 355 65 253 1.58e-14 73.6
Msa0183330 AT3G16800.6 29.515 227 110 9 141 355 65 253 1.58e-14 73.6
Msa0183330 AT4G31860.1 25.926 270 120 11 80 302 51 287 1.77e-14 74.7
Msa0183330 AT5G27930.1 28.837 215 106 8 149 354 177 353 5.31e-14 73.2
Msa0183330 AT5G27930.2 28.837 215 106 8 149 354 177 353 5.31e-14 73.2
Msa0183330 AT1G34750.4 24.818 274 152 9 19 291 17 237 2.74e-13 69.7
Msa0183330 AT2G34740.1 24.176 273 144 9 83 353 121 332 4.83e-13 70.1
Msa0183330 AT1G48040.1 25.904 332 162 14 49 357 81 351 8.68e-13 69.7
Msa0183330 AT1G79630.6 28.750 240 98 10 154 365 106 300 4.31e-12 67.8
Msa0183330 AT1G79630.3 28.750 240 98 10 154 365 106 300 4.31e-12 67.8
Msa0183330 AT1G79630.4 28.750 240 98 10 154 365 106 300 4.31e-12 67.8
Msa0183330 AT1G79630.5 28.750 240 98 10 154 365 106 300 4.31e-12 67.8
Msa0183330 AT1G79630.1 28.750 240 98 10 154 365 216 410 4.35e-12 68.2
Msa0183330 AT1G79630.8 28.750 240 98 10 154 365 212 406 4.40e-12 67.8
Msa0183330 AT1G79630.2 28.750 240 98 10 154 365 146 340 6.21e-12 67.4
Msa0183330 AT1G79630.7 28.750 240 98 10 154 365 146 340 6.21e-12 67.4
Msa0183330 AT1G18030.2 26.577 222 115 8 84 291 112 299 1.51e-11 65.5
Msa0183330 AT4G32950.1 26.255 259 151 10 79 325 70 300 4.68e-11 63.9
Msa0183330 AT2G40860.4 25.448 279 147 10 83 355 327 550 8.37e-11 63.9

Find 132 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTTAATTTAATTTAAT+TGG 0.103600 1_4:-72203627 Msa0183330:three_prime_UTR
TAGATCTGGGTATTTATTTA+TGG 0.142679 1_4:+72206932 None:intergenic
GATCTTACCAAAGAATTTAT+TGG 0.192729 1_4:+72206862 None:intergenic
TGGTGATTCCAGGGCTGTTT+TGG 0.205002 1_4:-72205067 Msa0183330:CDS
CACCAAGATTTGCTATATAA+AGG 0.237162 1_4:+72205085 None:intergenic
TCTTCATCCAATAAATTCTT+TGG 0.257491 1_4:-72206869 Msa0183330:intron
CTGATCTTCCAGCAAGTTAT+TGG 0.273921 1_4:+72206106 None:intergenic
TTGAATGAGTCTGGCCCTTA+TGG 0.294051 1_4:-72206056 Msa0183330:CDS
CTACTACTTTACTTGTGAAA+TGG 0.300020 1_4:+72207020 None:intergenic
TTCCATGGTATTTCTATTAG+AGG 0.311289 1_4:-72203726 Msa0183330:CDS
GGCCTGCTTTCGGCCGGGTT+CGG 0.311713 1_4:-72206240 Msa0183330:CDS
TACTCTTAAGATGTTGAAAT+AGG 0.323585 1_4:+72205970 None:intergenic
GGCCCTTATGGAACCTTTGT+TGG 0.326218 1_4:-72206044 Msa0183330:CDS
GCTGCAATTTGCGGTGAAAT+TGG 0.328123 1_4:+72205150 None:intergenic
TTATCTGTTGTAGTAGTAAA+AGG 0.336468 1_4:+72203669 None:intergenic
ATTGTTGTTGTGAGGATTGC+AGG 0.346192 1_4:-72206382 Msa0183330:five_prime_UTR
CAAATATAATAAATTGATCT+TGG 0.350049 1_4:+72204485 None:intergenic
TAGCAAATCTTGGTGATTCC+AGG 0.350723 1_4:-72205077 Msa0183330:CDS
TAATTTAATTTAATTTAATT+GGG 0.357456 1_4:-72203626 Msa0183330:three_prime_UTR
TACTACTTTACTTGTGAAAT+GGG 0.361108 1_4:+72207021 None:intergenic
ACCAAGATTTGCTATATAAA+GGG 0.370067 1_4:+72205086 None:intergenic
ACAACAATATTTGAATCATC+AGG 0.370480 1_4:+72204943 None:intergenic
TTAATGTCCACTTCATAAAC+AGG 0.370496 1_4:-72203490 Msa0183330:three_prime_UTR
CTTGTTGGTGTTATTTGTAA+TGG 0.371085 1_4:-72205111 Msa0183330:CDS
AAATATAATAAATTGATCTT+GGG 0.373950 1_4:+72204486 None:intergenic
ATAAAATTGACCGTGGAGTT+CGG 0.374935 1_4:-72203827 Msa0183330:CDS
GTTGGGTCTTGCTGTCTTGT+TGG 0.378018 1_4:-72205126 Msa0183330:CDS
ACCCTTTATATAGCAAATCT+TGG 0.384018 1_4:-72205087 Msa0183330:CDS
GCGTGTGAAATCATTAGATC+TGG 0.385126 1_4:+72206918 None:intergenic
TTTGAAGGCCTGCTTTCGGC+CGG 0.392079 1_4:-72206246 Msa0183330:CDS
GGAAGATTAGTTAAAGCAAC+TGG 0.395251 1_4:-72205045 Msa0183330:CDS
TTTATTATATTTGCATCTGA+TGG 0.398456 1_4:-72204475 Msa0183330:CDS
TATTTGCATCTGATGGGCTC+TGG 0.400975 1_4:-72204468 Msa0183330:CDS
TTGGTGAACCTCTGCTTAGC+TGG 0.401287 1_4:-72207102 Msa0183330:five_prime_UTR
GTAAACAAGATGGACTATTA+TGG 0.406307 1_4:-72206184 Msa0183330:CDS
AGTCGAAACTTGGCATATAA+TGG 0.416306 1_4:+72204574 None:intergenic
TTCCTCTCTCTATCTGATAG+TGG 0.417575 1_4:-72207179 Msa0183330:five_prime_UTR
TAATTGGGATCTATACTAGT+AGG 0.418748 1_4:-72203611 Msa0183330:three_prime_UTR
TGCTTTCGGCCGGGTTCGGA+TGG 0.422995 1_4:-72206236 Msa0183330:CDS
AGTTTGAGTTTGAATGAGTC+TGG 0.426624 1_4:-72206065 Msa0183330:CDS
TTGATAAACCGCGACGTCTC+AGG 0.427072 1_4:+72205999 None:intergenic
TGAAATCCTTGATGGTTGTT+TGG 0.428633 1_4:-72206303 Msa0183330:five_prime_UTR
GGTGAAATGAGGTATGTTAT+CGG 0.430907 1_4:-72206282 Msa0183330:exon
TTAGATGTTGAAATCCTTGA+TGG 0.431122 1_4:-72206311 Msa0183330:five_prime_UTR
GACCCAACTGCTGCAATTTG+CGG 0.431869 1_4:+72205141 None:intergenic
TTCAGACAGATTGTTAAAGA+TGG 0.434159 1_4:+72203527 None:intergenic
AGAAGAGGGGTTTATGTCAC+TGG 0.439976 1_4:-72205187 Msa0183330:CDS
AACTGATGCACCAGTATTGA+TGG 0.445954 1_4:+72204514 None:intergenic
TCACCGCAAATTGCAGCAGT+TGG 0.449592 1_4:-72205144 Msa0183330:CDS
ATTTGCATCTGATGGGCTCT+GGG 0.451624 1_4:-72204467 Msa0183330:CDS
GATGGTTCTGAGCTCAGTAT+TGG 0.453050 1_4:+72204532 None:intergenic
GATCAGAGCCAGATTGAATC+TGG 0.453389 1_4:-72206089 Msa0183330:CDS
TAAACCCCTCTTCTGTTGCT+TGG 0.457911 1_4:+72205196 None:intergenic
CACCGCAAATTGCAGCAGTT+GGG 0.458676 1_4:-72205143 Msa0183330:CDS
ATCCGAACCCGGCCGAAAGC+AGG 0.463029 1_4:+72206238 None:intergenic
CTTTACTTGTGAAATGGGAA+TGG 0.463533 1_4:+72207026 None:intergenic
AGTTCAAGCCAATAACTTGC+TGG 0.463611 1_4:-72206114 Msa0183330:CDS
TTGAAGGCCTGCTTTCGGCC+GGG 0.465199 1_4:-72206245 Msa0183330:CDS
CGGCTGAGGAAGTCGAAACT+TGG 0.465922 1_4:+72204564 None:intergenic
TTTACTTGTGAAATGGGAAT+GGG 0.467549 1_4:+72207027 None:intergenic
GAGCTCAGAACCATCAATAC+TGG 0.468725 1_4:-72204524 Msa0183330:CDS
ACCTTTGTTGGCGTCTATGA+TGG 0.469469 1_4:-72206032 Msa0183330:CDS
ACGCCAACAAAGGTTCCATA+AGG 0.475151 1_4:+72206041 None:intergenic
CCTCAGATTTCTAGATGCAT+TGG 0.482645 1_4:-72204634 Msa0183330:intron
TCAAACTACCAGATTCAATC+TGG 0.487886 1_4:+72206081 None:intergenic
GCGCGCACAACGTCAGATGC+TGG 0.493872 1_4:-72206209 Msa0183330:CDS
GGTATTTCTATTAGAGGAGG+TGG 0.499198 1_4:-72203720 Msa0183330:CDS
TTATTATATTTGCATCTGAT+GGG 0.499462 1_4:-72204474 Msa0183330:CDS
CTAAGCAGAGGTTCACCAAT+GGG 0.500506 1_4:+72207106 None:intergenic
CGCGCATATCCATCCGAACC+CGG 0.515703 1_4:+72206227 None:intergenic
TAATCTTCCCAAAACAGCCC+TGG 0.516311 1_4:+72205059 None:intergenic
CATGTTGTTGTAGTTTGTGG+TGG 0.520500 1_4:-72206423 Msa0183330:intron
CGCCAACAAAGGTTCCATAA+GGG 0.521754 1_4:+72206042 None:intergenic
ATCAATAGAATCTATAAGAC+AGG 0.522108 1_4:-72204983 Msa0183330:CDS
CTCAGTATTGGCCTCTTGAA+CGG 0.523336 1_4:+72204544 None:intergenic
ACTACTACAACAGATAATGA+TGG 0.526156 1_4:-72203663 Msa0183330:CDS
GAATGGGCAGGCAAGGAAGA+AGG 0.527492 1_4:+72207043 None:intergenic
GCATAATGTTTGGCGTGTGA+AGG 0.529364 1_4:-72204914 Msa0183330:CDS
CTATTATGGTATAAAGACTC+AGG 0.532513 1_4:-72206170 Msa0183330:CDS
CATAATGTTTGGCGTGTGAA+GGG 0.533025 1_4:-72204913 Msa0183330:CDS
GATGGTTGTTTGGTGAAATG+AGG 0.537890 1_4:-72206293 Msa0183330:five_prime_UTR
GCATTCCAAGCAACAGAAGA+GGG 0.537905 1_4:-72205201 Msa0183330:CDS
GACTCAGGGCAGCACATATG+TGG 0.544741 1_4:-72206155 Msa0183330:CDS
ATTTCACCAAACAACCATCA+AGG 0.547233 1_4:+72206297 None:intergenic
GACTTCCTCAGCCGTTCAAG+AGG 0.548513 1_4:-72204555 Msa0183330:CDS
ATTGGCCTCTTGAACGGCTG+AGG 0.549999 1_4:+72204550 None:intergenic
GGAGTAACATAGCATAGCAT+TGG 0.553344 1_4:-72207143 Msa0183330:five_prime_UTR
AAGAAGGAGTGAGGCAGACA+AGG 0.556916 1_4:+72207059 None:intergenic
TGTCACTGGTTTCGCAGCTG+TGG 0.558786 1_4:-72205173 Msa0183330:CDS
CATGGTATTTCTATTAGAGG+AGG 0.562847 1_4:-72203723 Msa0183330:CDS
TCAGGGAATTGCAAGGAGGA+TGG 0.563559 1_4:-72203907 Msa0183330:intron
CTATAGTGAGTGAAAGCTGT+AGG 0.567762 1_4:-72206334 Msa0183330:five_prime_UTR
CGTGTGAAATCATTAGATCT+GGG 0.567844 1_4:+72206919 None:intergenic
TCATCATCATTGTTGTTGTG+AGG 0.570191 1_4:-72206390 Msa0183330:five_prime_UTR
GCAAGCACTGTGAAGTTCCA+TGG 0.571337 1_4:-72203741 Msa0183330:CDS
CAATCACAGTGATATCATCA+TGG 0.573397 1_4:+72203796 None:intergenic
AGCAAATCTTGGTGATTCCA+GGG 0.574596 1_4:-72205076 Msa0183330:CDS
TAATGGTTCTCTATTGAACT+CGG 0.575111 1_4:+72204591 None:intergenic
CAACAATATTTGAATCATCA+GGG 0.575478 1_4:+72204944 None:intergenic
GTCAGTTGATGTAATTCGCA+AGG 0.576319 1_4:-72205223 Msa0183330:CDS
GATGCTGGAGGTAAACAAGA+TGG 0.576681 1_4:-72206194 Msa0183330:CDS
GATAGTGGTTCAATAAACAT+TGG 0.577778 1_4:-72207164 Msa0183330:five_prime_UTR
AATATAATAAATTGATCTTG+GGG 0.578298 1_4:+72204487 None:intergenic
GTGCTCTGTTGATAATTGCA+TGG 0.581373 1_4:+72205011 None:intergenic
CTTGTGAAATGGGAATGGGC+AGG 0.586442 1_4:+72207031 None:intergenic
GAAATGGGAATGGGCAGGCA+AGG 0.588709 1_4:+72207036 None:intergenic
TATTGTTTCAGGGAATTGCA+AGG 0.591708 1_4:-72203914 Msa0183330:intron
AATGAGTATCTTACAGCGCG+AGG 0.594025 1_4:+72204400 None:intergenic
CTCCTCTAATAGAAATACCA+TGG 0.594551 1_4:+72203724 None:intergenic
GGCATTCCAAGCAACAGAAG+AGG 0.600647 1_4:-72205202 Msa0183330:CDS
ATTAGTTAAAGCAACTGGTG+AGG 0.614946 1_4:-72205040 Msa0183330:CDS
GGAAATGTCGCCGAACTCCA+CGG 0.621058 1_4:+72203817 None:intergenic
GCTAAGCAGAGGTTCACCAA+TGG 0.624209 1_4:+72207105 None:intergenic
GATTTAAATAAAATTGACCG+TGG 0.627009 1_4:-72203834 Msa0183330:CDS
AACCACTATCAGATAGAGAG+AGG 0.632781 1_4:+72207177 None:intergenic
CATAGCATAGCATTGGAATG+TGG 0.634142 1_4:-72207136 Msa0183330:five_prime_UTR
GAATGTGGTGTGGAGCCCAT+TGG 0.634998 1_4:-72207121 Msa0183330:five_prime_UTR
GGTTGGAGCCAGCTAAGCAG+AGG 0.640777 1_4:+72207094 None:intergenic
CAGGCAAGGAAGAAGGAGTG+AGG 0.652455 1_4:+72207050 None:intergenic
TAGCATCCCTCACAACACAT+TGG 0.653156 1_4:-72203694 Msa0183330:CDS
CATTCCAAGCAACAGAAGAG+GGG 0.653186 1_4:-72205200 Msa0183330:CDS
TCCATCATAGACGCCAACAA+AGG 0.655109 1_4:+72206031 None:intergenic
CATAGCATTGGAATGTGGTG+TGG 0.657707 1_4:-72207131 Msa0183330:five_prime_UTR
GTTGGCGTCTATGATGGACA+TGG 0.658289 1_4:-72206026 Msa0183330:CDS
TATTATGGTATAAAGACTCA+GGG 0.658621 1_4:-72206169 Msa0183330:CDS
AAGGTGCCAATGTGTTGTGA+GGG 0.673166 1_4:+72203688 None:intergenic
AAAGGTGCCAATGTGTTGTG+AGG 0.683532 1_4:+72203687 None:intergenic
ATGGAGGTCCTGAGACGTCG+CGG 0.688834 1_4:-72206007 Msa0183330:CDS
CGCACAACGTCAGATGCTGG+AGG 0.694925 1_4:-72206206 Msa0183330:CDS
CAATCTGTCTGAAAGAACTG+TGG 0.707917 1_4:-72203517 Msa0183330:three_prime_UTR
TGTTTCAGGGAATTGCAAGG+AGG 0.712929 1_4:-72203911 Msa0183330:intron
GGCGTCTATGATGGACATGG+AGG 0.748970 1_4:-72206023 Msa0183330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTAATTTAATTTAAT+TGG - chr1_4:72207007-72207026 Msa0183330:five_prime_UTR 0.0%
!!! TAATTTAATTTAATTTAATT+GGG - chr1_4:72207008-72207027 Msa0183330:five_prime_UTR 0.0%
!! ACATATAAAAAAATCAATTA+TGG + chr1_4:72204180-72204199 None:intergenic 10.0%
!! AAATATAATAAATTGATCTT+GGG + chr1_4:72206151-72206170 None:intergenic 10.0%
!!! TTTTTAATCTTAACTAATAA+TGG + chr1_4:72206295-72206314 None:intergenic 10.0%
!!! GATTAAAATCTTGATTTTTA+TGG - chr1_4:72203831-72203850 Msa0183330:CDS 15.0%
!! ATAAACTGAAATATAAACTA+TGG + chr1_4:72204141-72204160 None:intergenic 15.0%
!!! ATAAATTCATATTTGGTAAT+CGG + chr1_4:72205337-72205356 None:intergenic 15.0%
!! AGATTGTAAAATTTATTACA+AGG + chr1_4:72205976-72205995 None:intergenic 15.0%
!! AATATAATAAATTGATCTTG+GGG + chr1_4:72206150-72206169 None:intergenic 15.0%
!! CAAATATAATAAATTGATCT+TGG + chr1_4:72206152-72206171 None:intergenic 15.0%
!! AAAAGAAGAAGAAGAAAAAA+GGG + chr1_4:72203678-72203697 None:intergenic 20.0%
!! AAAAAGAAGAAGAAGAAAAA+AGG + chr1_4:72203679-72203698 None:intergenic 20.0%
!! AGAAAGAAAATTGAGAAAAA+GGG + chr1_4:72203797-72203816 None:intergenic 20.0%
!!! TTCAGTTCTTTTTAATTGAA+AGG + chr1_4:72203962-72203981 None:intergenic 20.0%
!! GTTCTCTAATCATAAATAAA+AGG + chr1_4:72204077-72204096 None:intergenic 20.0%
!! TCTAGAAAGATTCAAAAAAT+AGG - chr1_4:72204802-72204821 Msa0183330:intron 20.0%
!!! TAAATTCATATTTGGTAATC+GGG + chr1_4:72205336-72205355 None:intergenic 20.0%
!!! TTGTTTTAAAGCATAATGTT+TGG - chr1_4:72205710-72205729 Msa0183330:intron 20.0%
!! ATCGCAAACAATTATAATAT+TGG - chr1_4:72205870-72205889 Msa0183330:intron 20.0%
!! TTTATTATATTTGCATCTGA+TGG - chr1_4:72206159-72206178 Msa0183330:CDS 20.0%
!! TTATTATATTTGCATCTGAT+GGG - chr1_4:72206160-72206179 Msa0183330:CDS 20.0%
!!! TTTTTAGACTGAAAACTTAA+GGG - chr1_4:72206330-72206349 Msa0183330:five_prime_UTR 20.0%
!!! TTTTTTGACTGAAAACTTAA+GGG - chr1_4:72206496-72206515 Msa0183330:intron 20.0%
!!! GATCACATATAATTTGTTAT+AGG - chr1_4:72206640-72206659 Msa0183330:intron 20.0%
!!! ATGTTTTATGTTATTGTTTC+AGG - chr1_4:72206709-72206728 Msa0183330:intron 20.0%
!!! TGTTTTATGTTATTGTTTCA+GGG - chr1_4:72206710-72206729 Msa0183330:intron 20.0%
!!! TTAAATTGCATATCTTTTGA+AGG - chr1_4:72207070-72207089 Msa0183330:five_prime_UTR 20.0%
! TACTACTTTACTTGTGAAAT+GGG + chr1_4:72203616-72203635 None:intergenic 25.0%
! AAAGAAGAAGAAGAAAAAAG+GGG + chr1_4:72203677-72203696 None:intergenic 25.0%
! TAGATCTGGGTATTTATTTA+TGG + chr1_4:72203705-72203724 None:intergenic 25.0%
! TCTTCATCCAATAAATTCTT+TGG - chr1_4:72203765-72203784 Msa0183330:CDS 25.0%
! GATCTTACCAAAGAATTTAT+TGG + chr1_4:72203775-72203794 None:intergenic 25.0%
! GAGAAAGAAAATTGAGAAAA+AGG + chr1_4:72203798-72203817 None:intergenic 25.0%
! CAATGTAAAAATGTCAACAA+TGG - chr1_4:72203882-72203901 Msa0183330:CDS 25.0%
!!! TTTTTTCTCTGACCAATTTT+TGG + chr1_4:72203928-72203947 None:intergenic 25.0%
! TATTATGGTATAAAGACTCA+GGG - chr1_4:72204465-72204484 Msa0183330:CDS 25.0%
! TACTCTTAAGATGTTGAAAT+AGG + chr1_4:72204667-72204686 None:intergenic 25.0%
!!! TTTTCTTGGTTCTAAAAGAT+GGG + chr1_4:72204775-72204794 None:intergenic 25.0%
!!! ATTTTCTTGGTTCTAAAAGA+TGG + chr1_4:72204776-72204795 None:intergenic 25.0%
!! TTCTAGATAAGTGATTTTCT+TGG + chr1_4:72204789-72204808 None:intergenic 25.0%
! ATGAGACAGAAATATTATCT+CGG + chr1_4:72204863-72204882 None:intergenic 25.0%
!! ATTCTTTTGTTGGTATATAC+TGG + chr1_4:72204892-72204911 None:intergenic 25.0%
!! TAAGAGAATGATTCTTTTGT+TGG + chr1_4:72204902-72204921 None:intergenic 25.0%
!! TTTTTGTGGCATTAATTCAA+TGG + chr1_4:72205018-72205037 None:intergenic 25.0%
!!! TGTATATCTGCTATTTTTTG+TGG + chr1_4:72205032-72205051 None:intergenic 25.0%
!!! GATTACAAAGTACTTTTCTT+GGG + chr1_4:72205123-72205142 None:intergenic 25.0%
!!! ACCAAGATTTGCTATATAAA+GGG + chr1_4:72205551-72205570 None:intergenic 25.0%
! ATCAATAGAATCTATAAGAC+AGG - chr1_4:72205651-72205670 Msa0183330:intron 25.0%
! CAACAATATTTGAATCATCA+GGG + chr1_4:72205693-72205712 None:intergenic 25.0%
! ACAACAATATTTGAATCATC+AGG + chr1_4:72205694-72205713 None:intergenic 25.0%
!! CTTTTTAGACTGAAAACTTA+AGG - chr1_4:72206329-72206348 Msa0183330:five_prime_UTR 25.0%
!! AACGTTTTTATTGCAAGTAT+CGG - chr1_4:72206366-72206385 Msa0183330:five_prime_UTR 25.0%
! TGAGTAATCTTGACTAATAA+TGG + chr1_4:72206429-72206448 None:intergenic 25.0%
!!! CTTTTTTGACTGAAAACTTA+AGG - chr1_4:72206495-72206514 Msa0183330:intron 25.0%
! AAAATTTGTGCGGATAATTT+AGG - chr1_4:72206592-72206611 Msa0183330:intron 25.0%
! TATGTGATCTCTATAAATCA+TGG + chr1_4:72206629-72206648 None:intergenic 25.0%
! GATTTAAATAAAATTGACCG+TGG - chr1_4:72206800-72206819 Msa0183330:intron 25.0%
! TTATCTGTTGTAGTAGTAAA+AGG + chr1_4:72206968-72206987 None:intergenic 25.0%
!!! ATAAACAGGATTTTTGTTTG+TGG - chr1_4:72207158-72207177 Msa0183330:five_prime_UTR 25.0%
! GATAGTGGTTCAATAAACAT+TGG - chr1_4:72203470-72203489 Msa0183330:three_prime_UTR 30.0%
TTTACTTGTGAAATGGGAAT+GGG + chr1_4:72203610-72203629 None:intergenic 30.0%
CTACTACTTTACTTGTGAAA+TGG + chr1_4:72203617-72203636 None:intergenic 30.0%
AAGAAGAAGAAGAAAAAAGG+GGG + chr1_4:72203676-72203695 None:intergenic 30.0%
ATATTGAGCTGACCAAAAAT+TGG - chr1_4:72203913-72203932 Msa0183330:intron 30.0%
AATTGGTCAGAGAAAAAAGT+TGG - chr1_4:72203930-72203949 Msa0183330:intron 30.0%
!! AGAACCCAATTTCTGTTTTT+GGG - chr1_4:72204092-72204111 Msa0183330:intron 30.0%
TTAGATGTTGAAATCCTTGA+TGG - chr1_4:72204323-72204342 Msa0183330:intron 30.0%
!! GTAAACAAGATGGACTATTA+TGG - chr1_4:72204450-72204469 Msa0183330:CDS 30.0%
CTATTATGGTATAAAGACTC+AGG - chr1_4:72204464-72204483 Msa0183330:CDS 30.0%
TCTCTTATCAGATAAACTGT+TGG - chr1_4:72204915-72204934 Msa0183330:CDS 30.0%
! CTGTTTAATTGCATGCATTT+TGG - chr1_4:72205081-72205100 Msa0183330:CDS 30.0%
!! GGATTACAAAGTACTTTTCT+TGG + chr1_4:72205124-72205143 None:intergenic 30.0%
TGACCAAACTTTACTTATCT+CGG + chr1_4:72205156-72205175 None:intergenic 30.0%
AGTAAAGTTTGGTCAAAGAT+TGG - chr1_4:72205161-72205180 Msa0183330:CDS 30.0%
CAGTTAATGTCGAATAATTC+AGG + chr1_4:72205214-72205233 None:intergenic 30.0%
AAACAAATGATTGAGCTAAG+TGG + chr1_4:72205248-72205267 None:intergenic 30.0%
CTTTCTCTACTTCAAATTTC+AGG - chr1_4:72205364-72205383 Msa0183330:intron 30.0%
!! CTTGTTGGTGTTATTTGTAA+TGG - chr1_4:72205523-72205542 Msa0183330:intron 30.0%
ACCCTTTATATAGCAAATCT+TGG - chr1_4:72205547-72205566 Msa0183330:intron 30.0%
!! CACCAAGATTTGCTATATAA+AGG + chr1_4:72205552-72205571 None:intergenic 30.0%
GTTCTTCATTTCATGAAACT+TGG - chr1_4:72205942-72205961 Msa0183330:intron 30.0%
ATTTCATGAAACTTGGAAGT+TGG - chr1_4:72205949-72205968 Msa0183330:intron 30.0%
TTTCATGAAACTTGGAAGTT+GGG - chr1_4:72205950-72205969 Msa0183330:intron 30.0%
TAATGGTTCTCTATTGAACT+CGG + chr1_4:72206046-72206065 None:intergenic 30.0%
! ATCGTTTTCATTGCAAGTAT+CGG - chr1_4:72206544-72206563 Msa0183330:intron 30.0%
GTTTGTGTACAAAATTTGTG+CGG - chr1_4:72206582-72206601 Msa0183330:intron 30.0%
GATAAGAGTTTAGTGCAAAA+TGG - chr1_4:72206681-72206700 Msa0183330:intron 30.0%
! TTCCATGGTATTTCTATTAG+AGG - chr1_4:72206908-72206927 Msa0183330:five_prime_UTR 30.0%
ACTACTACAACAGATAATGA+TGG - chr1_4:72206971-72206990 Msa0183330:five_prime_UTR 30.0%
TAATTGGGATCTATACTAGT+AGG - chr1_4:72207023-72207042 Msa0183330:five_prime_UTR 30.0%
TTCAGACAGATTGTTAAAGA+TGG + chr1_4:72207110-72207129 None:intergenic 30.0%
TTAATGTCCACTTCATAAAC+AGG - chr1_4:72207144-72207163 Msa0183330:five_prime_UTR 30.0%
CAAAAATCCTGTTTATGAAG+TGG + chr1_4:72207154-72207173 None:intergenic 30.0%
CTTTACTTGTGAAATGGGAA+TGG + chr1_4:72203611-72203630 None:intergenic 35.0%
AGAAGAAGAAGAAAAAAGGG+GGG + chr1_4:72203675-72203694 None:intergenic 35.0%
CGTGTGAAATCATTAGATCT+GGG + chr1_4:72203718-72203737 None:intergenic 35.0%
! ATACTTCTGTGACTTGTGTT+TGG - chr1_4:72203854-72203873 Msa0183330:CDS 35.0%
! GAGAACCCAATTTCTGTTTT+TGG - chr1_4:72204091-72204110 Msa0183330:intron 35.0%
GACACCCAAAAACAGAAATT+GGG + chr1_4:72204099-72204118 None:intergenic 35.0%
TGACACCCAAAAACAGAAAT+TGG + chr1_4:72204100-72204119 None:intergenic 35.0%
! TCATCATCATTGTTGTTGTG+AGG - chr1_4:72204244-72204263 Msa0183330:intron 35.0%
! TGAAATCCTTGATGGTTGTT+TGG - chr1_4:72204331-72204350 Msa0183330:intron 35.0%
ATTTCACCAAACAACCATCA+AGG + chr1_4:72204340-72204359 None:intergenic 35.0%
GGTGAAATGAGGTATGTTAT+CGG - chr1_4:72204352-72204371 Msa0183330:intron 35.0%
! GGAGTTAGTGAACTTTTTGA+AGG - chr1_4:72204373-72204392 Msa0183330:intron 35.0%
!! CATATGTGGTGATTTTTCGA+TGG - chr1_4:72204493-72204512 Msa0183330:CDS 35.0%
TCAAACTACCAGATTCAATC+TGG + chr1_4:72204556-72204575 None:intergenic 35.0%
! AGTTTGAGTTTGAATGAGTC+TGG - chr1_4:72204569-72204588 Msa0183330:CDS 35.0%
! ATAGAGTCTACTTGTTGCTT+TGG - chr1_4:72204741-72204760 Msa0183330:intron 35.0%
TACACGTACTAAGAAAACAG+TGG - chr1_4:72205048-72205067 Msa0183330:CDS 35.0%
GTACTTTGTAATCCAGAGTT+TGG - chr1_4:72205130-72205149 Msa0183330:CDS 35.0%
GAAAACACAAGTTCAAATCC+TGG + chr1_4:72205192-72205211 None:intergenic 35.0%
! GGAAGATTAGTTAAAGCAAC+TGG - chr1_4:72205589-72205608 Msa0183330:intron 35.0%
ATTAGTTAAAGCAACTGGTG+AGG - chr1_4:72205594-72205613 Msa0183330:intron 35.0%
CTATCAGAGACTATCTGTTA+TGG + chr1_4:72205827-72205846 None:intergenic 35.0%
AGAAAAGCCTTAAATTCCCT+TGG - chr1_4:72205905-72205924 Msa0183330:intron 35.0%
CACAAATCCAAGGGAATTTA+AGG + chr1_4:72205915-72205934 None:intergenic 35.0%
CAATGCATCTAGAAATCTGA+GGG + chr1_4:72206002-72206021 None:intergenic 35.0%
AGTCGAAACTTGGCATATAA+TGG + chr1_4:72206063-72206082 None:intergenic 35.0%
! TGAAAACTTAAGGGTATGTG+TGG - chr1_4:72206339-72206358 Msa0183330:five_prime_UTR 35.0%
TGAAAACTTAAGGGTCTGTA+TGG - chr1_4:72206505-72206524 Msa0183330:intron 35.0%
ATCTCTATAAATCATGGCAC+AGG + chr1_4:72206623-72206642 None:intergenic 35.0%
TATTGTTTCAGGGAATTGCA+AGG - chr1_4:72206720-72206739 Msa0183330:intron 35.0%
ATAAAATTGACCGTGGAGTT+CGG - chr1_4:72206807-72206826 Msa0183330:intron 35.0%
CAATCACAGTGATATCATCA+TGG + chr1_4:72206841-72206860 None:intergenic 35.0%
CTCCTCTAATAGAAATACCA+TGG + chr1_4:72206913-72206932 None:intergenic 35.0%
! CATGGTATTTCTATTAGAGG+AGG - chr1_4:72206911-72206930 Msa0183330:five_prime_UTR 35.0%
TTCCTCTCTCTATCTGATAG+TGG - chr1_4:72203455-72203474 Msa0183330:three_prime_UTR 40.0%
AACCACTATCAGATAGAGAG+AGG + chr1_4:72203460-72203479 None:intergenic 40.0%
GGAGTAACATAGCATAGCAT+TGG - chr1_4:72203491-72203510 Msa0183330:three_prime_UTR 40.0%
! CATAGCATAGCATTGGAATG+TGG - chr1_4:72203498-72203517 Msa0183330:three_prime_UTR 40.0%
CAAGGTCCATAAAAAGAGGT+TGG + chr1_4:72203560-72203579 None:intergenic 40.0%
CAGACAAGGTCCATAAAAAG+AGG + chr1_4:72203564-72203583 None:intergenic 40.0%
GAAGAAGAAGAAAAAAGGGG+GGG + chr1_4:72203674-72203693 None:intergenic 40.0%
GCGTGTGAAATCATTAGATC+TGG + chr1_4:72203719-72203738 None:intergenic 40.0%
TCAACTTTAGCTGCTGTAGA+TGG - chr1_4:72203996-72204015 Msa0183330:intron 40.0%
GTCCATGTTGTTGTAGTTTG+TGG - chr1_4:72204208-72204227 Msa0183330:intron 40.0%
CACCACAAACTACAACAACA+TGG + chr1_4:72204213-72204232 None:intergenic 40.0%
! CATGTTGTTGTAGTTTGTGG+TGG - chr1_4:72204211-72204230 Msa0183330:intron 40.0%
! ATTGTTGTTGTGAGGATTGC+AGG - chr1_4:72204252-72204271 Msa0183330:intron 40.0%
CTATAGTGAGTGAAAGCTGT+AGG - chr1_4:72204300-72204319 Msa0183330:intron 40.0%
! GATGGTTGTTTGGTGAAATG+AGG - chr1_4:72204341-72204360 Msa0183330:intron 40.0%
!! ACTTTTTGAAGGCCTGCTTT+CGG - chr1_4:72204384-72204403 Msa0183330:intron 40.0%
! AGTTCAAGCCAATAACTTGC+TGG - chr1_4:72204520-72204539 Msa0183330:CDS 40.0%
CTGATCTTCCAGCAAGTTAT+TGG + chr1_4:72204531-72204550 None:intergenic 40.0%
GGAGTTATGACCAATTGACT+AGG - chr1_4:72204986-72205005 Msa0183330:CDS 40.0%
ATGGTATGCTCCTAGTCAAT+TGG + chr1_4:72204999-72205018 None:intergenic 40.0%
! TACTTTTCTTGGGAACCTGA+GGG + chr1_4:72205113-72205132 None:intergenic 40.0%
TGGCCGAGATAAGTAAAGTT+TGG - chr1_4:72205150-72205169 Msa0183330:CDS 40.0%
GATTGAGCTAAGTGGTTGAT+GGG + chr1_4:72205240-72205259 None:intergenic 40.0%
TGATTGAGCTAAGTGGTTGA+TGG + chr1_4:72205241-72205260 None:intergenic 40.0%
! GTCAGTTGATGTAATTCGCA+AGG - chr1_4:72205411-72205430 Msa0183330:intron 40.0%
! TAGCAAATCTTGGTGATTCC+AGG - chr1_4:72205557-72205576 Msa0183330:intron 40.0%
! AGCAAATCTTGGTGATTCCA+GGG - chr1_4:72205558-72205577 Msa0183330:intron 40.0%
!! GTGCTCTGTTGATAATTGCA+TGG + chr1_4:72205626-72205645 None:intergenic 40.0%
CATAATGTTTGGCGTGTGAA+GGG - chr1_4:72205721-72205740 Msa0183330:intron 40.0%
CCAATGCATCTAGAAATCTG+AGG + chr1_4:72206003-72206022 None:intergenic 40.0%
! CCTCAGATTTCTAGATGCAT+TGG - chr1_4:72206000-72206019 Msa0183330:CDS 40.0%
! AACTGATGCACCAGTATTGA+TGG + chr1_4:72206123-72206142 None:intergenic 40.0%
! GGTATTTCTATTAGAGGAGG+TGG - chr1_4:72206914-72206933 Msa0183330:five_prime_UTR 40.0%
CAATCTGTCTGAAAGAACTG+TGG - chr1_4:72207117-72207136 Msa0183330:five_prime_UTR 40.0%
! CATAGCATTGGAATGTGGTG+TGG - chr1_4:72203503-72203522 Msa0183330:three_prime_UTR 45.0%
CTAAGCAGAGGTTCACCAAT+GGG + chr1_4:72203531-72203550 None:intergenic 45.0%
! CTGGCTCCAACCTCTTTTTA+TGG - chr1_4:72203551-72203570 Msa0183330:three_prime_UTR 45.0%
CTTTAGCTGCTGTAGATGGA+TGG - chr1_4:72204000-72204019 Msa0183330:intron 45.0%
GATGCTGGAGGTAAACAAGA+TGG - chr1_4:72204440-72204459 Msa0183330:CDS 45.0%
GATCAGAGCCAGATTGAATC+TGG - chr1_4:72204545-72204564 Msa0183330:CDS 45.0%
TTGAATGAGTCTGGCCCTTA+TGG - chr1_4:72204578-72204597 Msa0183330:CDS 45.0%
CGCCAACAAAGGTTCCATAA+GGG + chr1_4:72204595-72204614 None:intergenic 45.0%
ACGCCAACAAAGGTTCCATA+AGG + chr1_4:72204596-72204615 None:intergenic 45.0%
ACCTTTGTTGGCGTCTATGA+TGG - chr1_4:72204602-72204621 Msa0183330:CDS 45.0%
TCCATCATAGACGCCAACAA+AGG + chr1_4:72204606-72204625 None:intergenic 45.0%
! GAGTTCGAGTTTCAGCTCAA+TGG - chr1_4:72204961-72204980 Msa0183330:CDS 45.0%
TCGAGTTTCAGCTCAATGGT+TGG - chr1_4:72204965-72204984 Msa0183330:CDS 45.0%
!!! GCATTTTGGTCTTAACCCTC+AGG - chr1_4:72205095-72205114 Msa0183330:CDS 45.0%
! GTACTTTTCTTGGGAACCTG+AGG + chr1_4:72205114-72205133 None:intergenic 45.0%
TACTTATCTCGGCCAAACTC+TGG + chr1_4:72205145-72205164 None:intergenic 45.0%
GCATTCCAAGCAACAGAAGA+GGG - chr1_4:72205433-72205452 Msa0183330:intron 45.0%
CATTCCAAGCAACAGAAGAG+GGG - chr1_4:72205434-72205453 Msa0183330:intron 45.0%
TAAACCCCTCTTCTGTTGCT+TGG + chr1_4:72205441-72205460 None:intergenic 45.0%
AGAAGAGGGGTTTATGTCAC+TGG - chr1_4:72205447-72205466 Msa0183330:intron 45.0%
GCTGCAATTTGCGGTGAAAT+TGG + chr1_4:72205487-72205506 None:intergenic 45.0%
TAATCTTCCCAAAACAGCCC+TGG + chr1_4:72205578-72205597 None:intergenic 45.0%
GCATAATGTTTGGCGTGTGA+AGG - chr1_4:72205720-72205739 Msa0183330:intron 45.0%
ACACAAGCGCACAAATCCAA+GGG + chr1_4:72205924-72205943 None:intergenic 45.0%
AACACAAGCGCACAAATCCA+AGG + chr1_4:72205925-72205944 None:intergenic 45.0%
!! CTCAGTATTGGCCTCTTGAA+CGG + chr1_4:72206093-72206112 None:intergenic 45.0%
!! GATGGTTCTGAGCTCAGTAT+TGG + chr1_4:72206105-72206124 None:intergenic 45.0%
GAGCTCAGAACCATCAATAC+TGG - chr1_4:72206110-72206129 Msa0183330:CDS 45.0%
!! TATTTGCATCTGATGGGCTC+TGG - chr1_4:72206166-72206185 Msa0183330:CDS 45.0%
!! ATTTGCATCTGATGGGCTCT+GGG - chr1_4:72206167-72206186 Msa0183330:CDS 45.0%
AATGAGTATCTTACAGCGCG+AGG + chr1_4:72206237-72206256 None:intergenic 45.0%
!! TTGCAAGTATCGGTCATGCT+TGG - chr1_4:72206376-72206395 Msa0183330:five_prime_UTR 45.0%
!! TGCAAGTATCGGTCATGCTT+GGG - chr1_4:72206377-72206396 Msa0183330:five_prime_UTR 45.0%
TGTTTCAGGGAATTGCAAGG+AGG - chr1_4:72206723-72206742 Msa0183330:intron 45.0%
TAGCATCCCTCACAACACAT+TGG - chr1_4:72206940-72206959 Msa0183330:five_prime_UTR 45.0%
! AAGGTGCCAATGTGTTGTGA+GGG + chr1_4:72206949-72206968 None:intergenic 45.0%
!! AAAGGTGCCAATGTGTTGTG+AGG + chr1_4:72206950-72206969 None:intergenic 45.0%
!! AATATATATAAATTCATATT+TGG + chr1_4:72205344-72205363 None:intergenic 5.0%
GCTAAGCAGAGGTTCACCAA+TGG + chr1_4:72203532-72203551 None:intergenic 50.0%
TTGGTGAACCTCTGCTTAGC+TGG - chr1_4:72203532-72203551 Msa0183330:three_prime_UTR 50.0%
AAGAAGGAGTGAGGCAGACA+AGG + chr1_4:72203578-72203597 None:intergenic 50.0%
CTTGTGAAATGGGAATGGGC+AGG + chr1_4:72203606-72203625 None:intergenic 50.0%
GGCCCTTATGGAACCTTTGT+TGG - chr1_4:72204590-72204609 Msa0183330:CDS 50.0%
!! GTTGGCGTCTATGATGGACA+TGG - chr1_4:72204608-72204627 Msa0183330:CDS 50.0%
TTGATAAACCGCGACGTCTC+AGG + chr1_4:72204638-72204657 None:intergenic 50.0%
CTGTTGGAGCATAGATAGCG+AGG - chr1_4:72204931-72204950 Msa0183330:CDS 50.0%
!! GGTCAAAGATTGGTCTAGCC+AGG - chr1_4:72205171-72205190 Msa0183330:CDS 50.0%
GGCATTCCAAGCAACAGAAG+AGG - chr1_4:72205432-72205451 Msa0183330:intron 50.0%
TCACCGCAAATTGCAGCAGT+TGG - chr1_4:72205490-72205509 Msa0183330:intron 50.0%
CACCGCAAATTGCAGCAGTT+GGG - chr1_4:72205491-72205510 Msa0183330:intron 50.0%
GACCCAACTGCTGCAATTTG+CGG + chr1_4:72205496-72205515 None:intergenic 50.0%
! GTTGGGTCTTGCTGTCTTGT+TGG - chr1_4:72205508-72205527 Msa0183330:intron 50.0%
! TGGTGATTCCAGGGCTGTTT+TGG - chr1_4:72205567-72205586 Msa0183330:intron 50.0%
!! GGTGATTCCAGGGCTGTTTT+GGG - chr1_4:72205568-72205587 Msa0183330:intron 50.0%
!! AAGTATCGGTCATGCTTGGG+TGG - chr1_4:72206380-72206399 Msa0183330:five_prime_UTR 50.0%
TCAGGGAATTGCAAGGAGGA+TGG - chr1_4:72206727-72206746 Msa0183330:intron 50.0%
! GCAAGCACTGTGAAGTTCCA+TGG - chr1_4:72206893-72206912 Msa0183330:five_prime_UTR 50.0%
GAATGTGGTGTGGAGCCCAT+TGG - chr1_4:72203513-72203532 Msa0183330:three_prime_UTR 55.0%
CAGGCAAGGAAGAAGGAGTG+AGG + chr1_4:72203587-72203606 None:intergenic 55.0%
GAATGGGCAGGCAAGGAAGA+AGG + chr1_4:72203594-72203613 None:intergenic 55.0%
GAAATGGGAATGGGCAGGCA+AGG + chr1_4:72203601-72203620 None:intergenic 55.0%
TTTGAAGGCCTGCTTTCGGC+CGG - chr1_4:72204388-72204407 Msa0183330:intron 55.0%
GACTCAGGGCAGCACATATG+TGG - chr1_4:72204479-72204498 Msa0183330:CDS 55.0%
!! GGCGTCTATGATGGACATGG+AGG - chr1_4:72204611-72204630 Msa0183330:CDS 55.0%
!! TGTCACTGGTTTCGCAGCTG+TGG - chr1_4:72205461-72205480 Msa0183330:intron 55.0%
CGGCTGAGGAAGTCGAAACT+TGG + chr1_4:72206073-72206092 None:intergenic 55.0%
GACTTCCTCAGCCGTTCAAG+AGG - chr1_4:72206079-72206098 Msa0183330:CDS 55.0%
! ATTGGCCTCTTGAACGGCTG+AGG + chr1_4:72206087-72206106 None:intergenic 55.0%
GGAAATGTCGCCGAACTCCA+CGG + chr1_4:72206820-72206839 None:intergenic 55.0%
GGTTGGAGCCAGCTAAGCAG+AGG + chr1_4:72203543-72203562 None:intergenic 60.0%
TTGAAGGCCTGCTTTCGGCC+GGG - chr1_4:72204389-72204408 Msa0183330:intron 60.0%
CGCGCATATCCATCCGAACC+CGG + chr1_4:72204410-72204429 None:intergenic 60.0%
CGCACAACGTCAGATGCTGG+AGG - chr1_4:72204428-72204447 Msa0183330:CDS 60.0%
ATGGAGGTCCTGAGACGTCG+CGG - chr1_4:72204627-72204646 Msa0183330:CDS 60.0%
ATCCGAACCCGGCCGAAAGC+AGG + chr1_4:72204399-72204418 None:intergenic 65.0%
TGCTTTCGGCCGGGTTCGGA+TGG - chr1_4:72204398-72204417 Msa0183330:intron 65.0%
GCGCGCACAACGTCAGATGC+TGG - chr1_4:72204425-72204444 Msa0183330:CDS 65.0%
GGCCTGCTTTCGGCCGGGTT+CGG - chr1_4:72204394-72204413 Msa0183330:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 72203454 72207202 72203454 ID=Msa0183330;Name=Msa0183330
chr1_4 mRNA 72203454 72207202 72203454 ID=Msa0183330-mRNA-1;Parent=Msa0183330;Name=Msa0183330-mRNA-1;_AED=0.17;_eAED=0.17;_QI=474|1|1|1|0.75|0.6|5|202|397
chr1_4 exon 72203454 72203925 72203454 ID=Msa0183330-mRNA-1:exon:24386;Parent=Msa0183330-mRNA-1
chr1_4 exon 72204414 72204650 72204414 ID=Msa0183330-mRNA-1:exon:24385;Parent=Msa0183330-mRNA-1
chr1_4 exon 72204903 72205270 72204903 ID=Msa0183330-mRNA-1:exon:24384;Parent=Msa0183330-mRNA-1
chr1_4 exon 72205973 72206432 72205973 ID=Msa0183330-mRNA-1:exon:24383;Parent=Msa0183330-mRNA-1
chr1_4 exon 72206870 72207202 72206870 ID=Msa0183330-mRNA-1:exon:24382;Parent=Msa0183330-mRNA-1
chr1_4 five_prime_UTR 72206870 72207202 72206870 ID=Msa0183330-mRNA-1:five_prime_utr;Parent=Msa0183330-mRNA-1
chr1_4 five_prime_UTR 72206292 72206432 72206292 ID=Msa0183330-mRNA-1:five_prime_utr;Parent=Msa0183330-mRNA-1
chr1_4 CDS 72205973 72206291 72205973 ID=Msa0183330-mRNA-1:cds;Parent=Msa0183330-mRNA-1
chr1_4 CDS 72204903 72205270 72204903 ID=Msa0183330-mRNA-1:cds;Parent=Msa0183330-mRNA-1
chr1_4 CDS 72204414 72204650 72204414 ID=Msa0183330-mRNA-1:cds;Parent=Msa0183330-mRNA-1
chr1_4 CDS 72203656 72203925 72203656 ID=Msa0183330-mRNA-1:cds;Parent=Msa0183330-mRNA-1
chr1_4 three_prime_UTR 72203454 72203655 72203454 ID=Msa0183330-mRNA-1:three_prime_utr;Parent=Msa0183330-mRNA-1
Gene Sequence

>Msa0183330

ATGTTATCGGAGTTAGTGAACTTTTTGAAGGCCTGCTTTCGGCCGGGTTCGGATGGATATGCGCGCACAACGTCAGATGCTGGAGGTAAACAAGATGGACTATTATGGTATAAAGACTCAGGGCAGCACATATGTGGTGATTTTTCGATGGCTGTAGTTCAAGCCAATAACTTGCTGGAAGATCAGAGCCAGATTGAATCTGGTAGTTTGAGTTTGAATGAGTCTGGCCCTTATGGAACCTTTGTTGGCGTCTATGATGGACATGGAGGTCCTGAGACGTCGCGGTTTATCAACAATCACCTATTTCAACATCTTAAGAGATTTACTTCTGAGCAACAATCTATGTCAGTTGATGTAATTCGCAAGGCATTCCAAGCAACAGAAGAGGGGTTTATGTCACTGGTTTCGCAGCTGTGGCCAATTTCACCGCAAATTGCAGCAGTTGGGTCTTGCTGTCTTGTTGGTGTTATTTGTAATGGAACCCTTTATATAGCAAATCTTGGTGATTCCAGGGCTGTTTTGGGAAGATTAGTTAAAGCAACTGGTGAGGTTTTAGCCATGCAATTATCAACAGAGCACAATGCATCAATAGAATCTATAAGACAGGAGCTTCATTCTATGCACCCTGATGATTCAAATATTGTTGTTTTAAAGCATAATGTTTGGCGTGTGAAGGGAATTATTCAAATTTCTAGATGCATTGGCGATGTATATTTGAAAAAAGCCGAGTTCAATAGAGAACCATTATATGCCAAGTTTCGACTTCCTCAGCCGTTCAAGAGGCCAATACTGAGCTCAGAACCATCAATACTGGTGCATCAGTTGCAGCCCCAAGATCAATTTATTATATTTGCATCTGATGGGCTCTGGGAGCACTTCAGCAATCAAGAAGCAGTTGATATAGTTCAAAACAATCCTCGCGCTGGAATTGCAAGGAGGATGGTGAAAACTGCGCTTAAAGCAGCAGCAAAGAAGAGAGAAATGAGATATTCAGATTTAAATAAAATTGACCGTGGAGTTCGGCGACATTTCCATGATGATATCACTGTGATTGTTGTGTTTCTTGACTCAAATCTTATGAGTAGAGCAAGCACTGTGAAGTTCCATGGTATTTCTATTAGAGGAGGTGGTGTTAGCATCCCTCACAACACATTGGCACCTTTTACTACTACAACAGATAATGATGGTACTTGA
Protein sequence

>Msa0183330

MLSELVNFLKACFRPGSDGYARTTSDAGGKQDGLLWYKDSGQHICGDFSMAVVQANNLLEDQSQIESGSLSLNESGPYGTFVGVYDGHGGPETSRFINNHLFQHLKRFTSEQQSMSVDVIRKAFQATEEGFMSLVSQLWPISPQIAAVGSCCLVGVICNGTLYIANLGDSRAVLGRLVKATGEVLAMQLSTEHNASIESIRQELHSMHPDDSNIVVLKHNVWRVKGIIQISRCIGDVYLKKAEFNREPLYAKFRLPQPFKRPILSSEPSILVHQLQPQDQFIIFASDGLWEHFSNQEAVDIVQNNPRAGIARRMVKTALKAAAKKREMRYSDLNKIDRGVRRHFHDDITVIVVFLDSNLMSRASTVKFHGISIRGGGVSIPHNTLAPFTTTTDNDGT*