AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0190500

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190500 MtrunA17_Chr2g0280141 88.875 827 58 4 1 802 1 818 0.0 1394
Msa0190500 MtrunA17_Chr2g0325811 45.018 833 365 15 19 800 40 830 1.99e-180 539
Msa0190500 MtrunA17_Chr2g0325711 42.892 816 394 14 10 782 31 817 2.09e-178 533
Msa0190500 MtrunA17_Chr2g0325661 42.986 884 354 18 16 801 45 876 1.62e-166 504
Msa0190500 MtrunA17_Chr2g0325861 44.233 737 287 11 94 726 130 846 9.17e-159 483
Msa0190500 MtrunA17_Chr2g0325361 48.339 602 263 7 247 801 477 1077 1.16e-135 429
Msa0190500 MtrunA17_Chr2g0325361 35.636 550 294 9 25 528 13 548 1.13e-57 213
Msa0190500 MtrunA17_Chr6g0471251 39.204 829 346 20 19 801 51 767 3.45e-134 417
Msa0190500 MtrunA17_Chr2g0325321 41.365 747 351 12 41 703 18 761 1.95e-126 396
Msa0190500 MtrunA17_Chr2g0325231 36.303 898 359 21 19 720 16 896 2.90e-123 392
Msa0190500 MtrunA17_Chr2g0325611 51.173 469 189 9 358 801 137 590 7.18e-123 382
Msa0190500 MtrunA17_Chr2g0325611 37.262 263 108 7 187 426 93 321 3.48e-25 111
Msa0190500 MtrunA17_Chr2g0325351 45.629 572 268 11 246 801 144 688 4.29e-116 367
Msa0190500 MtrunA17_Chr2g0325351 36.336 333 142 6 92 413 142 415 1.88e-31 131
Msa0190500 MtrunA17_Chr2g0325621 42.831 551 284 8 271 801 121 660 1.15e-107 344
Msa0190500 MtrunA17_Chr2g0325621 34.554 437 200 8 12 414 3 387 8.59e-39 153
Msa0190500 MtrunA17_Chr5g0434761 61.132 265 99 4 540 801 2 265 6.03e-92 289
Msa0190500 MtrunA17_Chr2g0325701 38.213 526 238 11 182 700 144 589 6.43e-77 260
Msa0190500 MtrunA17_Chr2g0325631 59.245 265 104 4 540 801 2 265 1.36e-76 249
Msa0190500 MtrunA17_Chr3g0140591 74.405 168 42 1 536 703 6 172 2.56e-75 242
Msa0190500 MtrunA17_Chr2g0325281 37.607 585 276 10 41 538 18 600 5.21e-73 250
Msa0190500 MtrunA17_Chr5g0416271 38.241 489 246 10 215 700 47 482 4.58e-70 239
Msa0190500 MtrunA17_Chr5g0416271 33.832 334 178 8 42 363 14 316 2.45e-26 113
Msa0190500 MtrunA17_Chr5g0416271 34.444 270 148 5 186 452 78 321 8.65e-24 105
Msa0190500 MtrunA17_Chr2g0325291 64.878 205 69 3 555 757 3 206 5.30e-70 229
Msa0190500 MtrunA17_Chr2g0325221 35.387 568 295 8 19 529 45 597 8.04e-70 243
Msa0190500 MtrunA17_Chr2g0325221 61.386 101 38 1 701 800 599 699 3.79e-28 120
Msa0190500 MtrunA17_Chr2g0333341 38.758 467 237 14 269 720 189 621 2.80e-64 226
Msa0190500 MtrunA17_Chr2g0333341 35.917 387 194 10 25 366 72 449 5.85e-36 144
Msa0190500 MtrunA17_Chr4g0044221 57.672 189 79 1 81 268 1 189 3.58e-55 192
Msa0190500 MtrunA17_Chr4g0044221 63.226 155 28 2 485 638 168 294 2.14e-54 191
Msa0190500 MtrunA17_Chr2g0325241 41.538 325 162 6 311 626 120 425 2.36e-51 185
Msa0190500 MtrunA17_Chr2g0325241 36.585 205 125 2 247 448 126 328 7.33e-20 93.2
Msa0190500 MtrunA17_Chr2g0332961 43.522 301 128 9 383 673 85 353 9.52e-47 170
Msa0190500 MtrunA17_Chr2g0325721 35.217 531 238 12 22 504 29 501 1.47e-46 174
Msa0190500 MtrunA17_Chr2g0325761 30.769 650 331 12 21 605 53 648 6.45e-45 172
Msa0190500 MtrunA17_Chr2g0325761 62.245 98 36 1 700 796 591 688 2.48e-28 121
Msa0190500 MtrunA17_Chr2g0332951 47.807 228 94 8 482 703 2 210 2.37e-44 159
Msa0190500 MtrunA17_Chr2g0325771 58.046 174 61 3 533 703 11 175 8.27e-42 150
Msa0190500 MtrunA17_Chr8g0361651 34.673 398 231 11 416 799 23 405 1.00e-40 156
Msa0190500 MtrunA17_Chr4g0024061 30.395 658 345 26 190 799 59 651 1.20e-38 154
Msa0190500 MtrunA17_Chr2g0325331 58.170 153 62 2 541 692 3 154 2.04e-38 140
Msa0190500 MtrunA17_Chr2g0325651 32.547 424 183 10 21 408 30 386 2.25e-37 144
Msa0190500 MtrunA17_Chr2g0325651 45.833 144 68 2 389 525 237 377 4.31e-22 99.4
Msa0190500 MtrunA17_Chr2g0325651 45.033 151 72 2 374 513 236 386 3.70e-21 96.7
Msa0190500 MtrunA17_Chr2g0325691 33.906 466 219 13 121 526 283 719 6.39e-37 148
Msa0190500 MtrunA17_Chr2g0325691 32.189 466 266 9 52 511 63 484 4.43e-30 127
Msa0190500 MtrunA17_Chr2g0325691 37.132 272 149 4 295 551 223 487 1.23e-24 110
Msa0190500 MtrunA17_Chr7g0235901 36.034 358 170 5 60 414 10 311 1.90e-35 137
Msa0190500 MtrunA17_Chr7g0235901 35.484 217 102 2 295 511 132 310 6.54e-19 88.6
Msa0190500 MtrunA17_Chr7g0235901 34.694 196 123 2 338 529 92 286 2.15e-12 68.9
Msa0190500 MtrunA17_Chr2g0325311 63.107 103 36 2 701 801 17 119 1.60e-31 119
Msa0190500 MtrunA17_Chr3g0140581 55.147 136 59 1 91 226 21 154 1.54e-29 114
Msa0190500 MtrunA17_Chr2g0325641 36.336 333 189 5 211 537 3 318 7.79e-29 118
Msa0190500 MtrunA17_Chr2g0325641 36.364 275 168 1 248 522 1 268 1.63e-28 117
Msa0190500 MtrunA17_Chr2g0325641 37.795 254 133 5 295 541 6 241 4.48e-23 101
Msa0190500 MtrunA17_Chr2g0325641 34.615 260 132 5 189 417 58 310 5.64e-21 95.1
Msa0190500 MtrunA17_Chr2g0325271 54.808 104 45 2 700 801 40 143 5.94e-27 107
Msa0190500 MtrunA17_Chr2g0325201 35.865 237 137 2 117 353 2 223 3.33e-26 107
Msa0190500 MtrunA17_Chr2g0325781 32.011 353 125 9 16 340 40 305 1.87e-22 99.0
Msa0190500 MtrunA17_Chr5g0434741 31.373 306 123 7 55 325 46 299 3.00e-22 98.2
Msa0190500 MtrunA17_Chr5g0434751 37.143 280 148 2 248 513 1 266 1.28e-21 96.3
Msa0190500 MtrunA17_Chr5g0434751 35.146 239 137 3 187 418 35 262 6.06e-15 76.3
Msa0190500 MtrunA17_Chr5g0434751 37.156 218 123 2 247 457 56 266 9.02e-14 72.8
Msa0190500 MtrunA17_Chr7g0235921 59.794 97 38 1 617 713 1 96 2.10e-20 87.0
Msa0190500 MtrunA17_Chr2g0325261 29.859 355 121 7 55 374 45 306 3.85e-20 92.0
Msa0190500 MtrunA17_Chr2g0325261 51.220 82 40 0 374 455 222 303 4.95e-12 67.8
Msa0190500 MtrunA17_Chr2g0325341 29.859 355 121 7 55 374 45 306 3.85e-20 92.0
Msa0190500 MtrunA17_Chr2g0325341 51.220 82 40 0 374 455 222 303 4.95e-12 67.8
Msa0190500 MtrunA17_Chr4g0053711 32.143 252 145 8 575 802 194 443 4.54e-20 94.7
Msa0190500 MtrunA17_Chr2g0325741 29.480 346 117 11 16 317 42 304 1.84e-17 84.3
Msa0190500 MtrunA17_Chr2g0325791 46.032 126 58 1 309 434 41 156 4.77e-16 80.1
Msa0190500 MtrunA17_Chr2g0325791 38.889 126 67 1 351 476 41 156 1.54e-12 69.7
Msa0190500 MtrunA17_Chr2g0325751 36.242 149 94 1 402 549 7 155 5.75e-16 75.9
Msa0190500 MtrunA17_Chr2g0325751 40.764 157 44 3 248 404 7 114 3.99e-15 73.6
Msa0190500 MtrunA17_Chr2g0325751 45.217 115 56 2 304 418 7 114 4.40e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0190500 AT5G27220.1 28.079 203 134 7 580 777 872 1067 4.37e-15 80.5
Msa0190500 AT5G27220.1 25.542 646 406 20 75 696 208 802 1.11e-13 75.9
Msa0190500 AT5G27220.3 28.079 203 134 7 580 777 872 1067 4.37e-15 80.5
Msa0190500 AT5G27220.3 25.542 646 406 20 75 696 208 802 1.11e-13 75.9
Msa0190500 AT5G27220.4 25.542 646 406 20 75 696 208 802 6.32e-14 76.6
Msa0190500 AT5G27220.4 27.815 151 101 4 629 777 868 1012 5.66e-12 70.1
Msa0190500 AT5G27220.2 25.542 646 406 20 75 696 208 802 6.32e-14 76.6
Msa0190500 AT5G27220.2 27.815 151 101 4 629 777 868 1012 5.66e-12 70.1
Msa0190500 AT1G31814.1 26.786 224 150 6 568 782 117 335 1.69e-13 74.3
Msa0190500 AT5G48385.1 27.333 150 108 1 654 802 308 457 2.38e-13 73.9
Msa0190500 AT5G27230.1 27.056 377 212 16 422 754 38 395 3.56e-13 73.9

Find 161 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCATTGTCAACTTTGAATT+TGG 0.089700 2_1:+5999450 Msa0190500:CDS
TTGCTCCTTCTGTCAATTTA+TGG 0.102499 2_1:+6001411 Msa0190500:CDS
GATAGATAGTATTCTAATTT+TGG 0.129006 2_1:+6002660 Msa0190500:three_prime_UTR
CCAAAGTGAAACACTGTATT+TGG 0.182293 2_1:-5998714 None:intergenic
GGTTTGGCACATAAAGTTTC+TGG 0.195386 2_1:+6001525 Msa0190500:CDS
GCTCTTCTTCATGAGCTTAA+TGG 0.209076 2_1:-5999599 None:intergenic
CAGGATTCTGCGTTAAGATT+TGG 0.231497 2_1:+6002614 Msa0190500:three_prime_UTR
TGTAGAGCTGTTTGGGACTA+TGG 0.236978 2_1:+6001503 Msa0190500:CDS
TTTCTTATTTAGAAGCTCTT+TGG 0.268782 2_1:-5999689 None:intergenic
TCAAATGAGAAGCTCTATAA+AGG 0.280877 2_1:+6000201 Msa0190500:CDS
GTAGTTCAATAATGGTTGTT+AGG 0.295009 2_1:+5999350 Msa0190500:exon
AAGATTTGGGATTCAGTTCT+TGG 0.304088 2_1:+6002628 Msa0190500:three_prime_UTR
AGTTCATCAATTTCCTCAAT+TGG 0.304511 2_1:-6001111 None:intergenic
AGGATTCTGCGTTAAGATTT+GGG 0.318411 2_1:+6002615 Msa0190500:three_prime_UTR
TGCAAATCCAGTACCAGTTC+TGG 0.327369 2_1:+6001383 Msa0190500:CDS
CAAATACAGTGTTTCACTTT+GGG 0.336751 2_1:+5998715 Msa0190500:five_prime_UTR
ATGCAGAACAAAATCGAAAA+AGG 0.349065 2_1:+5999757 Msa0190500:CDS
CCTTCTGTCAATTTATGGAT+TGG 0.349385 2_1:+6001416 Msa0190500:CDS
TCATAATCAAAACATCAAAA+TGG 0.358355 2_1:-6002832 None:intergenic
CTTCCATCAATGGCAGGACT+TGG 0.360593 2_1:-6001072 None:intergenic
TCGGTCACACTGCTTCATTT+TGG 0.361776 2_1:-6000160 None:intergenic
TCATTGTCAACTTTGAATTT+GGG 0.364609 2_1:+5999451 Msa0190500:CDS
TCATCAAAAGGCCTCTTTGC+TGG 0.367743 2_1:-5999526 None:intergenic
AGATATCTGTAGAGCTGTTT+GGG 0.367948 2_1:+6001496 Msa0190500:CDS
AAGATATCTGTAGAGCTGTT+TGG 0.368664 2_1:+6001495 Msa0190500:CDS
GCTGTTTGGGACTATGGGTT+TGG 0.369030 2_1:+6001509 Msa0190500:CDS
CCCCATCATTCCACAGTGTA+AGG 0.370588 2_1:+6001218 Msa0190500:CDS
CAACCAAGTCCTGCCATTGA+TGG 0.374788 2_1:+6001069 Msa0190500:CDS
AGAAGAGCATACTCGGATTA+AGG 0.374809 2_1:+5999614 Msa0190500:CDS
TTTGAAGAGGAAATTGAATC+AGG 0.376038 2_1:+6000930 Msa0190500:CDS
TCTGCATTTCCTCGTAATAT+TGG 0.377971 2_1:-5999741 None:intergenic
TTCTCAGACTAGCAATTTCC+TGG 0.380055 2_1:-6002442 None:intergenic
TGGGATTCAGTTCTTGGATG+TGG 0.380091 2_1:+6002634 Msa0190500:three_prime_UTR
GAGAGAAAGGCGAGTAGCTT+TGG 0.383992 2_1:+6002549 Msa0190500:exon
CCCTTACACTGTGGAATGAT+GGG 0.386689 2_1:-6001219 None:intergenic
GTTCATCAATTTCCTCAATT+GGG 0.386900 2_1:-6001110 None:intergenic
TGATTTGTGTAGTTCAATAA+TGG 0.390713 2_1:+5999342 Msa0190500:exon
TCCCTTACACTGTGGAATGA+TGG 0.397453 2_1:-6001220 None:intergenic
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 2_1:+6000757 Msa0190500:CDS
AGGACTTTGAAAGCCGAGTT+AGG 0.398394 2_1:+6000799 Msa0190500:CDS
GACTTTGAAAGCCGAGTAAA+GGG 0.402680 2_1:+6000633 Msa0190500:CDS
CAGTGTGACCGACGACTAGA+AGG 0.410719 2_1:+6000171 Msa0190500:CDS
CCAAATACAGTGTTTCACTT+TGG 0.420774 2_1:+5998714 Msa0190500:five_prime_UTR
TAGTTCAATAATGGTTGTTA+GGG 0.427649 2_1:+5999351 Msa0190500:exon
GTAGAGCTGTTTGGGACTAT+GGG 0.430125 2_1:+6001504 Msa0190500:CDS
AAGGCGAGTAGCTTTGGTAT+TGG 0.430765 2_1:+6002555 Msa0190500:exon
TCAATCAGTTGATCTATTGC+GGG 0.431042 2_1:+6002246 Msa0190500:CDS
CTCTTTGCTGGCAGTCTAGT+AGG 0.431702 2_1:-5999514 None:intergenic
AACCTTGATTTCAATGGAGT+TGG 0.432488 2_1:-6002317 None:intergenic
TTGATTTGAGTCCCTTCAGT+TGG 0.434408 2_1:-6000392 None:intergenic
GATATTTGAAAGACGGATGA+AGG 0.437782 2_1:+6000422 Msa0190500:CDS
CTGTTTCAAACTTCGTCACT+CGG 0.440261 2_1:-6000686 None:intergenic
AACCAACTCCATTGAAATCA+AGG 0.448934 2_1:+6002315 Msa0190500:CDS
TCAAAGACAAGTAGATGATC+TGG 0.452367 2_1:+6000344 Msa0190500:CDS
GAAATATTGAGATAACATTA+CGG 0.455477 2_1:+6002914 Msa0190500:three_prime_UTR
TTTGCAGTTTCTCCCAATTG+AGG 0.456677 2_1:+6001098 Msa0190500:CDS
CTGATTTGAACTTCCTCACT+CGG 0.460825 2_1:-6000728 None:intergenic
GCCTTTACTATTGCTATCCA+TGG 0.462841 2_1:-5999884 None:intergenic
GTAGCTTTGGTATTGGAAGT+AGG 0.465926 2_1:+6002562 Msa0190500:exon
TAATCAAACCCTCGATGAAA+CGG 0.467409 2_1:-5999813 None:intergenic
TACTTACCTTTGTGTGGCTG+AGG 0.468990 2_1:-5998822 None:intergenic
ACTGTGGAATGATGGGGTTC+TGG 0.470582 2_1:-6001212 None:intergenic
GTAATGTCAGAGAGGAGAAC+TGG 0.471534 2_1:+6000066 Msa0190500:CDS
GTTGTCTCATTTACAAACTT+CGG 0.474404 2_1:-6002592 None:intergenic
AGGATGACAGTAATGAGAAC+TGG 0.474459 2_1:+6002759 Msa0190500:three_prime_UTR
GTAGTAAATCTATTGAGAAT+AGG 0.474756 2_1:+5999491 Msa0190500:CDS
GCAAATCCAGTACCAGTTCT+GGG 0.480024 2_1:+6001384 Msa0190500:CDS
GCAGGAGGAATTCGAAAGTC+AGG 0.484190 2_1:+6000248 Msa0190500:CDS
GCAATTTGAAAGCCAGGAAA+AGG 0.485355 2_1:+6000296 Msa0190500:CDS
CTCATGAAGAAGAGCATACT+CGG 0.485463 2_1:+5999607 Msa0190500:CDS
CAAATGAGAAGCTCTATAAA+GGG 0.486869 2_1:+6000202 Msa0190500:CDS
CTGCAAACTTCTTCCATCAA+TGG 0.487566 2_1:-6001082 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.487972 2_1:-6000812 None:intergenic
GCTTGTTCAAATGAAGGACT+TGG 0.488868 2_1:+6000092 Msa0190500:CDS
AAGTAGGAATGAAATGAGAA+AGG 0.489576 2_1:-5998785 None:intergenic
CTGATTCAAACTTCCTAACT+CGG 0.490086 2_1:-6000770 None:intergenic
AAAGAAGCAATTTGAAAGCC+AGG 0.492303 2_1:+6000290 Msa0190500:CDS
GAAGTTTGAATCAGTAGAGA+AGG 0.504108 2_1:+6000779 Msa0190500:CDS
TTGATTCAAACCCCTTTACT+CGG 0.505676 2_1:-6000644 None:intergenic
AAGTTTGTAAATGAGACAAC+AGG 0.507654 2_1:+6002595 Msa0190500:three_prime_UTR
GGGATTCAGTTCTTGGATGT+GGG 0.508374 2_1:+6002635 Msa0190500:three_prime_UTR
TAGCTTTGGTATTGGAAGTA+GGG 0.508548 2_1:+6002563 Msa0190500:exon
GAAGTTTGAATCAGCAGAGA+AGG 0.509904 2_1:+6000821 Msa0190500:CDS
GGACTTTGAAAGCCGAGTAA+AGG 0.510913 2_1:+6000632 Msa0190500:CDS
TAAAGGCCGTGGAAGATTGA+AGG 0.511019 2_1:+5999900 Msa0190500:CDS
ATCAATCAGTTGATCTATTG+CGG 0.512272 2_1:+6002245 Msa0190500:CDS
TTGATTCGAAGTCCTTCCCC+TGG 0.514391 2_1:-6000518 None:intergenic
GGAGAGTGAACTAAGAAAGT+AGG 0.514540 2_1:-5998801 None:intergenic
GAAGTTCAAATCAGTAGAGA+AGG 0.514891 2_1:+6000737 Msa0190500:CDS
GTTAATAAAATCATTTGAAG+AGG 0.515437 2_1:+6000917 Msa0190500:CDS
TTGATTCAAACTTCTTCACT+CGG 0.515510 2_1:-6000854 None:intergenic
TCAAAATTGAAGCAATATGA+AGG 0.517246 2_1:+6000537 Msa0190500:CDS
GCGCATGTCCGTTTCATCGA+GGG 0.520061 2_1:+5999805 Msa0190500:CDS
TCAATATTTCAAAGCAATTG+AGG 0.523088 2_1:-6002901 None:intergenic
GAGGAAATGCTGTCATCAAA+AGG 0.524403 2_1:-5999538 None:intergenic
CTTGAGCTAAATAGAGAGAA+AGG 0.526158 2_1:+6002536 Msa0190500:CDS
ATCCCTTGAGAGAGATATTG+AGG 0.530827 2_1:+5999660 Msa0190500:CDS
CAGCATTTCCTCGCAAACTT+CGG 0.531960 2_1:+5999549 Msa0190500:CDS
TAAAAGAACTAATGTCAGAA+AGG 0.536181 2_1:+6000028 Msa0190500:CDS
AAAGGTGTCGGAGTCAAAGC+AGG 0.537649 2_1:+6000230 Msa0190500:CDS
GAAGTTTGAATCAAAAGAAG+AGG 0.538867 2_1:+6000863 Msa0190500:CDS
TGGTCAGCTTGTTCAAATGA+AGG 0.542980 2_1:+6000086 Msa0190500:CDS
ATTCAACCCCTTCTAGTCGT+CGG 0.546192 2_1:-6000179 None:intergenic
CTTAAACTGAAAGCTTACAT+TGG 0.548401 2_1:+6001351 Msa0190500:CDS
AGAGTTTAAATCACAAGAGA+AGG 0.548434 2_1:+6000611 Msa0190500:CDS
TCAAAGAGAGAGGAATTTGA+AGG 0.551665 2_1:+6000579 Msa0190500:CDS
GACAATGACTTTGCTCTCAA+AGG 0.551969 2_1:-5999436 None:intergenic
ACTCCTCAATATCTCTCTCA+AGG 0.552126 2_1:-5999663 None:intergenic
TCAGGACAAGGCAAGAGACC+AGG 0.554657 2_1:+6002424 Msa0190500:intron
AAACTTCTTCCATCAATGGC+AGG 0.555447 2_1:-6001078 None:intergenic
ACAATTTACTTACCTTTGTG+TGG 0.555870 2_1:-5998828 None:intergenic
AGATAACAATATTGAATCTG+CGG 0.559408 2_1:+6002484 Msa0190500:CDS
AAGAGAAGATATTTGAAAGA+CGG 0.560688 2_1:+6000415 Msa0190500:CDS
TCAACGGAAAAGCAATTTGA+AGG 0.560873 2_1:+6000117 Msa0190500:CDS
ATTGCTTCTTCTCGCACATG+AGG 0.561232 2_1:-6001318 None:intergenic
GCAATATGAAGGACAAGTGA+AGG 0.566613 2_1:+6000548 Msa0190500:CDS
GAAAGCCAGGAAAAGGTGCT+CGG 0.567009 2_1:+6000303 Msa0190500:CDS
AAGTGAAACACTGTATTTGG+TGG 0.570485 2_1:-5998711 None:intergenic
TCAGGAAATTAAGACGAGAA+AGG 0.571467 2_1:+5999966 Msa0190500:CDS
GAAGTTTGAAACAGTAGAGA+AGG 0.571865 2_1:+6000695 Msa0190500:CDS
GGAATTCGAAAGTCAGGTGA+AGG 0.576331 2_1:+6000254 Msa0190500:CDS
GTGATTTAAACTCTTCCACT+CGG 0.576446 2_1:-6000602 None:intergenic
GATAGCAATAGTAAAGGCCG+TGG 0.579238 2_1:+5999889 Msa0190500:CDS
CGGATTAAGGAGCGTTGAGG+AGG 0.584542 2_1:+5999627 Msa0190500:CDS
TTTCTGCCCAGGATTTGAAG+TGG 0.585302 2_1:+5999319 Msa0190500:intron
GAAGGAGTTGAAGTCAAAAG+AGG 0.587532 2_1:+6000440 Msa0190500:CDS
CGCACATGAGGTTTAACATG+TGG 0.590019 2_1:-6001306 None:intergenic
CCAATCCATAAATTGACAGA+AGG 0.591953 2_1:-6001416 None:intergenic
GGAGTTACAATCAAAGAGAG+AGG 0.595701 2_1:+6000569 Msa0190500:CDS
GCCATGGATAGCAATAGTAA+AGG 0.596670 2_1:+5999883 Msa0190500:CDS
GAGACAACTTCTTACAGCCA+TGG 0.598031 2_1:+5999867 Msa0190500:CDS
CCCATCATTCCACAGTGTAA+GGG 0.604052 2_1:+6001219 Msa0190500:CDS
AATGAAGGACTTGGAGTCAA+CGG 0.606158 2_1:+6000101 Msa0190500:CDS
CCTTACACTGTGGAATGATG+GGG 0.610080 2_1:-6001218 None:intergenic
CTGATCGAGACACAAGGGGA+AGG 0.618054 2_1:+6002739 Msa0190500:three_prime_UTR
TAAAGGGCAGACAAAGGTGT+CGG 0.621702 2_1:+6000218 Msa0190500:CDS
CTCCTCAATATCTCTCTCAA+GGG 0.622421 2_1:-5999662 None:intergenic
TAGATTCATTTGTTATAACA+CGG 0.623887 2_1:+6002213 Msa0190500:CDS
AGTGTGACCGACGACTAGAA+GGG 0.627275 2_1:+6000172 Msa0190500:CDS
TTTATCTGATCGAGACACAA+GGG 0.627670 2_1:+6002734 Msa0190500:three_prime_UTR
ATTTATCTGATCGAGACACA+AGG 0.627865 2_1:+6002733 Msa0190500:three_prime_UTR
GAGGAACTTGAGCTAAGAGA+CGG 0.629178 2_1:+6000882 Msa0190500:CDS
AGAGGAATTTGAAGGCCGAG+TGG 0.631001 2_1:+6000587 Msa0190500:CDS
GCTCTATAAAGGGCAGACAA+AGG 0.633152 2_1:+6000212 Msa0190500:CDS
AGATGATCTGGAATCATGCA+AGG 0.638630 2_1:+6000356 Msa0190500:CDS
CACTCTCCTCAGCCACACAA+AGG 0.642678 2_1:+5998816 Msa0190500:five_prime_UTR
ACGGAAAAGCAATTTGAAGG+TGG 0.647692 2_1:+6000120 Msa0190500:CDS
AGCGCATGTCCGTTTCATCG+AGG 0.649306 2_1:+5999804 Msa0190500:CDS
CAAATCCAGTACCAGTTCTG+GGG 0.656039 2_1:+6001385 Msa0190500:CDS
GTGTGACCGACGACTAGAAG+GGG 0.661302 2_1:+6000173 Msa0190500:CDS
CTACTAGACTGCCAGCAAAG+AGG 0.670048 2_1:+5999515 Msa0190500:CDS
CTTTGGTATTGGAAGTAGGG+CGG 0.670313 2_1:+6002566 Msa0190500:three_prime_UTR
TACTAAATGTAATGTCAGAG+AGG 0.673630 2_1:+6000058 Msa0190500:CDS
AGGACTTTGAAATCCGAGTG+AGG 0.681019 2_1:+6000715 Msa0190500:CDS
ACTTTGAAAGCCGAGTAAAG+GGG 0.687533 2_1:+6000634 Msa0190500:CDS
ACTCGGATTAAGGAGCGTTG+AGG 0.688313 2_1:+5999624 Msa0190500:CDS
GGTGTCGGAGTCAAAGCAGG+AGG 0.695615 2_1:+6000233 Msa0190500:CDS
TTATCTGATCGAGACACAAG+GGG 0.696466 2_1:+6002735 Msa0190500:three_prime_UTR
ATTGATGAACTTGAGTCACA+CGG 0.701580 2_1:+6001123 Msa0190500:CDS
ACGAACTAACCAATATTACG+AGG 0.703439 2_1:+5999732 Msa0190500:CDS
GTTGTTCGAGCAGATCAATG+TGG 0.704073 2_1:-6001269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATTAAAAATTTATTGAA+TGG - chr2_1:5998928-5998947 None:intergenic 10.0%
!! ATCAAATGTATAAATATATT+TGG + chr2_1:6001665-6001684 Msa0190500:intron 10.0%
!! TTATAAAAAAGTTAGCATAT+TGG + chr2_1:6001812-6001831 Msa0190500:intron 15.0%
!! ATAACATCTCAAAATAAATT+GGG - chr2_1:6002098-6002117 None:intergenic 15.0%
!! AATAACATCTCAAAATAAAT+TGG - chr2_1:6002099-6002118 None:intergenic 15.0%
!! ATTATTTATAAATAACATGC+AGG - chr2_1:6002392-6002411 None:intergenic 15.0%
!!! AATAATTTCAATTTGAAATC+AGG + chr2_1:6002406-6002425 Msa0190500:intron 15.0%
!!! TTTTATCATTTACATTTCCA+TGG + chr2_1:5998892-5998911 Msa0190500:intron 20.0%
!! ATTTGCAGCTTATATATAAA+CGG - chr2_1:5999224-5999243 None:intergenic 20.0%
!!! ATTAAGTTGTTTATCCAAAA+AGG + chr2_1:5999247-5999266 Msa0190500:intron 20.0%
!!! TTAAGTTGTTTATCCAAAAA+GGG + chr2_1:5999248-5999267 Msa0190500:intron 20.0%
!! GTTAATAAAATCATTTGAAG+AGG + chr2_1:6000917-6000936 Msa0190500:CDS 20.0%
!!! TATCTAAAACATCTTTTGAT+GGG - chr2_1:6001188-6001207 None:intergenic 20.0%
!!! ATATCTAAAACATCTTTTGA+TGG - chr2_1:6001189-6001208 None:intergenic 20.0%
!!! ATGAAGTTTTAAAGCTTTTT+GGG + chr2_1:6001457-6001476 Msa0190500:CDS 20.0%
!!! ACAAATCAGAATTAACTTTT+GGG - chr2_1:6001793-6001812 None:intergenic 20.0%
!!! AACAAATCAGAATTAACTTT+TGG - chr2_1:6001794-6001813 None:intergenic 20.0%
!! ATATATCTTTCTAATTCAAG+TGG + chr2_1:6001867-6001886 Msa0190500:intron 20.0%
!! TAACATCTCAAAATAAATTG+GGG - chr2_1:6002097-6002116 None:intergenic 20.0%
!!! TAGATTCATTTGTTATAACA+CGG + chr2_1:6002213-6002232 Msa0190500:CDS 20.0%
!! AAAAAAAACCTTGATTTCAA+TGG - chr2_1:6002326-6002345 None:intergenic 20.0%
!!! GATAGATAGTATTCTAATTT+TGG + chr2_1:6002660-6002679 Msa0190500:three_prime_UTR 20.0%
!!! ATAGTATTCTAATTTTGGAA+TGG + chr2_1:6002665-6002684 Msa0190500:three_prime_UTR 20.0%
!! TCATAATCAAAACATCAAAA+TGG - chr2_1:6002835-6002854 None:intergenic 20.0%
!! GAAATATTGAGATAACATTA+CGG + chr2_1:6002914-6002933 Msa0190500:three_prime_UTR 20.0%
!! TGATTTGTGTAGTTCAATAA+TGG + chr2_1:5999342-5999361 Msa0190500:exon 25.0%
! TAGTTCAATAATGGTTGTTA+GGG + chr2_1:5999351-5999370 Msa0190500:exon 25.0%
! TCATTGTCAACTTTGAATTT+GGG + chr2_1:5999451-5999470 Msa0190500:CDS 25.0%
! GTAGTAAATCTATTGAGAAT+AGG + chr2_1:5999491-5999510 Msa0190500:CDS 25.0%
!! TTTCTTATTTAGAAGCTCTT+TGG - chr2_1:5999692-5999711 None:intergenic 25.0%
! TAAAAGAACTAATGTCAGAA+AGG + chr2_1:6000028-6000047 Msa0190500:CDS 25.0%
! AAGAGAAGATATTTGAAAGA+CGG + chr2_1:6000415-6000434 Msa0190500:CDS 25.0%
! TCAAAATTGAAGCAATATGA+AGG + chr2_1:6000537-6000556 Msa0190500:CDS 25.0%
!!! GATGAAGTTTTAAAGCTTTT+TGG + chr2_1:6001456-6001475 Msa0190500:CDS 25.0%
! AAATTGAACTGATATGGAAT+TGG + chr2_1:6001726-6001745 Msa0190500:intron 25.0%
!! AAATCAGAATTAACTTTTGG+GGG - chr2_1:6001791-6001810 None:intergenic 25.0%
!! CAAATCAGAATTAACTTTTG+GGG - chr2_1:6001792-6001811 None:intergenic 25.0%
! TGGTTAAAGAGTTTCAATTA+AGG + chr2_1:6001887-6001906 Msa0190500:intron 25.0%
!!! CATATGTGCTGTTTTTTTAA+TGG + chr2_1:6002050-6002069 Msa0190500:intron 25.0%
!!! TAGATCAACTGATTGATTTT+TGG - chr2_1:6002242-6002261 None:intergenic 25.0%
! AGATAACAATATTGAATCTG+CGG + chr2_1:6002484-6002503 Msa0190500:CDS 25.0%
! AACAAACTGATGTAAAAATC+TGG + chr2_1:6002790-6002809 Msa0190500:three_prime_UTR 25.0%
! TCAATATTTCAAAGCAATTG+AGG - chr2_1:6002904-6002923 None:intergenic 25.0%
CAAATACAGTGTTTCACTTT+GGG + chr2_1:5998715-5998734 Msa0190500:five_prime_UTR 30.0%
AAGTAGGAATGAAATGAGAA+AGG - chr2_1:5998788-5998807 None:intergenic 30.0%
ACAATTTACTTACCTTTGTG+TGG - chr2_1:5998831-5998850 None:intergenic 30.0%
TATCAATCACAAATCAGCTA+GGG - chr2_1:5998982-5999001 None:intergenic 30.0%
! ATTAGTCTAAGAAAACATGG+GGG - chr2_1:5999011-5999030 None:intergenic 30.0%
! CATTAGTCTAAGAAAACATG+GGG - chr2_1:5999012-5999031 None:intergenic 30.0%
! GCATTAGTCTAAGAAAACAT+GGG - chr2_1:5999013-5999032 None:intergenic 30.0%
! TGCATTAGTCTAAGAAAACA+TGG - chr2_1:5999014-5999033 None:intergenic 30.0%
CTTATAACATGAGATCTGTT+TGG + chr2_1:5999048-5999067 Msa0190500:intron 30.0%
!! AATAGTATCAAAGGTGCTTA+TGG - chr2_1:5999101-5999120 None:intergenic 30.0%
! TCAACTATTAAGACCCTTTT+TGG - chr2_1:5999264-5999283 None:intergenic 30.0%
GTAGTTCAATAATGGTTGTT+AGG + chr2_1:5999350-5999369 Msa0190500:exon 30.0%
GTCATTGTCAACTTTGAATT+TGG + chr2_1:5999450-5999469 Msa0190500:CDS 30.0%
ATGCAGAACAAAATCGAAAA+AGG + chr2_1:5999757-5999776 Msa0190500:CDS 30.0%
AAAAATTGCTGAATGTACTC+AGG + chr2_1:5999948-5999967 Msa0190500:CDS 30.0%
TACTAAATGTAATGTCAGAG+AGG + chr2_1:6000058-6000077 Msa0190500:CDS 30.0%
TCAAATGAGAAGCTCTATAA+AGG + chr2_1:6000201-6000220 Msa0190500:CDS 30.0%
CAAATGAGAAGCTCTATAAA+GGG + chr2_1:6000202-6000221 Msa0190500:CDS 30.0%
!! TTAAGAGAGTGTGATTTTGA+AGG + chr2_1:6000495-6000514 Msa0190500:CDS 30.0%
AGAGTTTAAATCACAAGAGA+AGG + chr2_1:6000611-6000630 Msa0190500:CDS 30.0%
TTGATTCAAACTTCTTCACT+CGG - chr2_1:6000857-6000876 None:intergenic 30.0%
GAAGTTTGAATCAAAAGAAG+AGG + chr2_1:6000863-6000882 Msa0190500:CDS 30.0%
TTTGAAGAGGAAATTGAATC+AGG + chr2_1:6000930-6000949 Msa0190500:CDS 30.0%
GATGAAATTGCATAATTAGC+TGG - chr2_1:6001036-6001055 None:intergenic 30.0%
GTTCATCAATTTCCTCAATT+GGG - chr2_1:6001113-6001132 None:intergenic 30.0%
AGTTCATCAATTTCCTCAAT+TGG - chr2_1:6001114-6001133 None:intergenic 30.0%
CTTAAACTGAAAGCTTACAT+TGG + chr2_1:6001351-6001370 Msa0190500:CDS 30.0%
CAATGTGAAGAAAGACAAAA+AGG - chr2_1:6001591-6001610 None:intergenic 30.0%
TTCTTATCACTTCTAAGTGT+AGG + chr2_1:6001627-6001646 Msa0190500:intron 30.0%
CCATATCAGTTCAATTTCTT+AGG - chr2_1:6001723-6001742 None:intergenic 30.0%
CCTAAGAAATTGAACTGATA+TGG + chr2_1:6001720-6001739 Msa0190500:intron 30.0%
TCAATTAAGGACGAATCATT+TGG + chr2_1:6001900-6001919 Msa0190500:intron 30.0%
CAATTAAGGACGAATCATTT+GGG + chr2_1:6001901-6001920 Msa0190500:intron 30.0%
ATATAAGAAAAGCTTGACCA+AGG - chr2_1:6001959-6001978 None:intergenic 30.0%
! GTCAAGCTTTTCTTATATCT+TGG + chr2_1:6001961-6001980 Msa0190500:intron 30.0%
!!! GCACATATGCATAGTTTTTT+TGG - chr2_1:6002039-6002058 None:intergenic 30.0%
!!! GCTGTTTTTTTAATGGTTTG+AGG + chr2_1:6002057-6002076 Msa0190500:intron 30.0%
ACTTCATCAAACTAACATAC+GGG - chr2_1:6002125-6002144 None:intergenic 30.0%
CACTTCATCAAACTAACATA+CGG - chr2_1:6002126-6002145 None:intergenic 30.0%
!! ATCAACTGATTGATTTTTGG+TGG - chr2_1:6002239-6002258 None:intergenic 30.0%
ATCAATCAGTTGATCTATTG+CGG + chr2_1:6002245-6002264 Msa0190500:CDS 30.0%
! TTCAATTTGAAATCAGGACA+AGG + chr2_1:6002412-6002431 Msa0190500:intron 30.0%
GTTGTCTCATTTACAAACTT+CGG - chr2_1:6002595-6002614 None:intergenic 30.0%
AAGTTTGTAAATGAGACAAC+AGG + chr2_1:6002595-6002614 Msa0190500:three_prime_UTR 30.0%
!!! GCTAACTCTTTTGTTTTTGT+TGG - chr2_1:6002882-6002901 None:intergenic 30.0%
CCAAAGTGAAACACTGTATT+TGG - chr2_1:5998717-5998736 None:intergenic 35.0%
CCAAATACAGTGTTTCACTT+TGG + chr2_1:5998714-5998733 Msa0190500:five_prime_UTR 35.0%
!!! TGAATGGATGCTATTTTCCA+TGG - chr2_1:5998912-5998931 None:intergenic 35.0%
CTATCAATCACAAATCAGCT+AGG - chr2_1:5998983-5999002 None:intergenic 35.0%
GTTCGCTCAAATAGTATCAA+AGG - chr2_1:5999110-5999129 None:intergenic 35.0%
TTTGAGCGAACTTATGAAGT+TGG + chr2_1:5999119-5999138 Msa0190500:intron 35.0%
!!! TCTTTTTTCTTTTTCTGCCC+AGG + chr2_1:5999308-5999327 Msa0190500:intron 35.0%
AAATCACCACTTCAAATCCT+GGG - chr2_1:5999328-5999347 None:intergenic 35.0%
!!! TTTTTTAACCGAAGTTTGCG+AGG - chr2_1:5999560-5999579 None:intergenic 35.0%
ACGAACTAACCAATATTACG+AGG + chr2_1:5999732-5999751 Msa0190500:CDS 35.0%
TCTGCATTTCCTCGTAATAT+TGG - chr2_1:5999744-5999763 None:intergenic 35.0%
TAATCAAACCCTCGATGAAA+CGG - chr2_1:5999816-5999835 None:intergenic 35.0%
! CTTTTTCCTTCAATCTTCCA+CGG - chr2_1:5999909-5999928 None:intergenic 35.0%
TCAGGAAATTAAGACGAGAA+AGG + chr2_1:5999966-5999985 Msa0190500:CDS 35.0%
TCAACGGAAAAGCAATTTGA+AGG + chr2_1:6000117-6000136 Msa0190500:CDS 35.0%
AAAGAAGCAATTTGAAAGCC+AGG + chr2_1:6000290-6000309 Msa0190500:CDS 35.0%
TCAAAGACAAGTAGATGATC+TGG + chr2_1:6000344-6000363 Msa0190500:CDS 35.0%
!! CATTTTGAAAGCCAACTGAA+GGG + chr2_1:6000381-6000400 Msa0190500:CDS 35.0%
GATATTTGAAAGACGGATGA+AGG + chr2_1:6000422-6000441 Msa0190500:CDS 35.0%
! ACGTTTTCAAAGACGAATGA+AGG + chr2_1:6000464-6000483 Msa0190500:CDS 35.0%
TCAAAGAGAGAGGAATTTGA+AGG + chr2_1:6000579-6000598 Msa0190500:CDS 35.0%
GTGATTTAAACTCTTCCACT+CGG - chr2_1:6000605-6000624 None:intergenic 35.0%
TTGATTCAAACCCCTTTACT+CGG - chr2_1:6000647-6000666 None:intergenic 35.0%
GGGGTTTGAATCAAAAAAGA+AGG + chr2_1:6000653-6000672 Msa0190500:CDS 35.0%
! GAAGTTTGAAACAGTAGAGA+AGG + chr2_1:6000695-6000714 Msa0190500:CDS 35.0%
GAAGTTCAAATCAGTAGAGA+AGG + chr2_1:6000737-6000756 Msa0190500:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr2_1:6000773-6000792 None:intergenic 35.0%
GAAGTTTGAATCAGTAGAGA+AGG + chr2_1:6000779-6000798 Msa0190500:CDS 35.0%
CTGATTCAAACTTCCTAACT+CGG - chr2_1:6000773-6000792 None:intergenic 35.0%
ATTGATGAACTTGAGTCACA+CGG + chr2_1:6001123-6001142 Msa0190500:CDS 35.0%
TTGCTCCTTCTGTCAATTTA+TGG + chr2_1:6001411-6001430 Msa0190500:CDS 35.0%
CCAATCCATAAATTGACAGA+AGG - chr2_1:6001419-6001438 None:intergenic 35.0%
CCTTCTGTCAATTTATGGAT+TGG + chr2_1:6001416-6001435 Msa0190500:CDS 35.0%
AAGATATCTGTAGAGCTGTT+TGG + chr2_1:6001495-6001514 Msa0190500:CDS 35.0%
AGATATCTGTAGAGCTGTTT+GGG + chr2_1:6001496-6001515 Msa0190500:CDS 35.0%
TAAGTGTAGGTTACATCAAG+TGG + chr2_1:6001640-6001659 Msa0190500:intron 35.0%
!!! TTTTTTTGGTCGTAACACTC+CGG - chr2_1:6002025-6002044 None:intergenic 35.0%
GATTCTGAACTAAATCTGCA+AGG - chr2_1:6002162-6002181 None:intergenic 35.0%
TCAATCAGTTGATCTATTGC+GGG + chr2_1:6002246-6002265 Msa0190500:CDS 35.0%
! ATTTTATGAGAGCAGTTGCA+AGG + chr2_1:6002291-6002310 Msa0190500:CDS 35.0%
AACCAACTCCATTGAAATCA+AGG + chr2_1:6002315-6002334 Msa0190500:CDS 35.0%
AACCTTGATTTCAATGGAGT+TGG - chr2_1:6002320-6002339 None:intergenic 35.0%
CTTGAGCTAAATAGAGAGAA+AGG + chr2_1:6002536-6002555 Msa0190500:CDS 35.0%
!! TAGCTTTGGTATTGGAAGTA+GGG + chr2_1:6002563-6002582 Msa0190500:exon 35.0%
AGGATTCTGCGTTAAGATTT+GGG + chr2_1:6002615-6002634 Msa0190500:three_prime_UTR 35.0%
! AAGATTTGGGATTCAGTTCT+TGG + chr2_1:6002628-6002647 Msa0190500:three_prime_UTR 35.0%
ATTTATCTGATCGAGACACA+AGG + chr2_1:6002733-6002752 Msa0190500:three_prime_UTR 35.0%
TTTATCTGATCGAGACACAA+GGG + chr2_1:6002734-6002753 Msa0190500:three_prime_UTR 35.0%
GGAGAGTGAACTAAGAAAGT+AGG - chr2_1:5998804-5998823 None:intergenic 40.0%
CAAATCACCACTTCAAATCC+TGG - chr2_1:5999329-5999348 None:intergenic 40.0%
GACAATGACTTTGCTCTCAA+AGG - chr2_1:5999439-5999458 None:intergenic 40.0%
GAGGAAATGCTGTCATCAAA+AGG - chr2_1:5999541-5999560 None:intergenic 40.0%
GCTCTTCTTCATGAGCTTAA+TGG - chr2_1:5999602-5999621 None:intergenic 40.0%
! CTCATGAAGAAGAGCATACT+CGG + chr2_1:5999607-5999626 Msa0190500:CDS 40.0%
! AGAAGAGCATACTCGGATTA+AGG + chr2_1:5999614-5999633 Msa0190500:CDS 40.0%
ATCCCTTGAGAGAGATATTG+AGG + chr2_1:5999660-5999679 Msa0190500:CDS 40.0%
CTCCTCAATATCTCTCTCAA+GGG - chr2_1:5999665-5999684 None:intergenic 40.0%
ACTCCTCAATATCTCTCTCA+AGG - chr2_1:5999666-5999685 None:intergenic 40.0%
GCCATGGATAGCAATAGTAA+AGG + chr2_1:5999883-5999902 Msa0190500:CDS 40.0%
GCCTTTACTATTGCTATCCA+TGG - chr2_1:5999887-5999906 None:intergenic 40.0%
! TGGTCAGCTTGTTCAAATGA+AGG + chr2_1:6000086-6000105 Msa0190500:CDS 40.0%
GCTTGTTCAAATGAAGGACT+TGG + chr2_1:6000092-6000111 Msa0190500:CDS 40.0%
AATGAAGGACTTGGAGTCAA+CGG + chr2_1:6000101-6000120 Msa0190500:CDS 40.0%
ACGGAAAAGCAATTTGAAGG+TGG + chr2_1:6000120-6000139 Msa0190500:CDS 40.0%
GCAATTTGAAAGCCAGGAAA+AGG + chr2_1:6000296-6000315 Msa0190500:CDS 40.0%
! AGATGATCTGGAATCATGCA+AGG + chr2_1:6000356-6000375 Msa0190500:CDS 40.0%
!! GCATTTTGAAAGCCAACTGA+AGG + chr2_1:6000380-6000399 Msa0190500:CDS 40.0%
! TTGATTTGAGTCCCTTCAGT+TGG - chr2_1:6000395-6000414 None:intergenic 40.0%
GAAGGAGTTGAAGTCAAAAG+AGG + chr2_1:6000440-6000459 Msa0190500:CDS 40.0%
GCAATATGAAGGACAAGTGA+AGG + chr2_1:6000548-6000567 Msa0190500:CDS 40.0%
GGAGTTACAATCAAAGAGAG+AGG + chr2_1:6000569-6000588 Msa0190500:CDS 40.0%
! GACTTTGAAAGCCGAGTAAA+GGG + chr2_1:6000633-6000652 Msa0190500:CDS 40.0%
! ACTTTGAAAGCCGAGTAAAG+GGG + chr2_1:6000634-6000653 Msa0190500:CDS 40.0%
CTGTTTCAAACTTCGTCACT+CGG - chr2_1:6000689-6000708 None:intergenic 40.0%
! CTGATTTGAACTTCCTCACT+CGG - chr2_1:6000731-6000750 None:intergenic 40.0%
GAAGTTTGAATCAGCAGAGA+AGG + chr2_1:6000821-6000840 Msa0190500:CDS 40.0%
AAACTTCTTCCATCAATGGC+AGG - chr2_1:6001081-6001100 None:intergenic 40.0%
CTGCAAACTTCTTCCATCAA+TGG - chr2_1:6001085-6001104 None:intergenic 40.0%
TTTGCAGTTTCTCCCAATTG+AGG + chr2_1:6001098-6001117 Msa0190500:CDS 40.0%
! ACATTTTCTCCCTTACACTG+TGG - chr2_1:6001231-6001250 None:intergenic 40.0%
GGTTTGGCACATAAAGTTTC+TGG + chr2_1:6001525-6001544 Msa0190500:CDS 40.0%
ATCTTGGCAAGAACAATCCT+TGG + chr2_1:6001939-6001958 Msa0190500:intron 40.0%
CTTATATCTTGGTCGAACTC+TGG + chr2_1:6001972-6001991 Msa0190500:intron 40.0%
! TACCAAAACCCTTTTCCCTT+AGG + chr2_1:6001997-6002016 Msa0190500:intron 40.0%
! TTCCTAAGGGAAAAGGGTTT+TGG - chr2_1:6002002-6002021 None:intergenic 40.0%
TTCTCAGACTAGCAATTTCC+TGG - chr2_1:6002445-6002464 None:intergenic 40.0%
!! GTAGCTTTGGTATTGGAAGT+AGG + chr2_1:6002562-6002581 Msa0190500:exon 40.0%
CAGGATTCTGCGTTAAGATT+TGG + chr2_1:6002614-6002633 Msa0190500:three_prime_UTR 40.0%
TTATCTGATCGAGACACAAG+GGG + chr2_1:6002735-6002754 Msa0190500:three_prime_UTR 40.0%
AGGATGACAGTAATGAGAAC+TGG + chr2_1:6002759-6002778 Msa0190500:three_prime_UTR 40.0%
TACTTACCTTTGTGTGGCTG+AGG - chr2_1:5998825-5998844 None:intergenic 45.0%
TTTCTGCCCAGGATTTGAAG+TGG + chr2_1:5999319-5999338 Msa0190500:intron 45.0%
TCATCAAAAGGCCTCTTTGC+TGG - chr2_1:5999529-5999548 None:intergenic 45.0%
CAGCATTTCCTCGCAAACTT+CGG + chr2_1:5999549-5999568 Msa0190500:CDS 45.0%
GAGACAACTTCTTACAGCCA+TGG + chr2_1:5999867-5999886 Msa0190500:CDS 45.0%
GATAGCAATAGTAAAGGCCG+TGG + chr2_1:5999889-5999908 Msa0190500:CDS 45.0%
TAAAGGCCGTGGAAGATTGA+AGG + chr2_1:5999900-5999919 Msa0190500:CDS 45.0%
GTAATGTCAGAGAGGAGAAC+TGG + chr2_1:6000066-6000085 Msa0190500:CDS 45.0%
! TCGGTCACACTGCTTCATTT+TGG - chr2_1:6000163-6000182 None:intergenic 45.0%
ATTCAACCCCTTCTAGTCGT+CGG - chr2_1:6000182-6000201 None:intergenic 45.0%
GCTCTATAAAGGGCAGACAA+AGG + chr2_1:6000212-6000231 Msa0190500:CDS 45.0%
! TAAAGGGCAGACAAAGGTGT+CGG + chr2_1:6000218-6000237 Msa0190500:CDS 45.0%
GGAATTCGAAAGTCAGGTGA+AGG + chr2_1:6000254-6000273 Msa0190500:CDS 45.0%
! TTAATCCGAGCACCTTTTCC+TGG - chr2_1:6000311-6000330 None:intergenic 45.0%
!! AGAGTGTGATTTTGAAGGCC+AGG + chr2_1:6000500-6000519 Msa0190500:CDS 45.0%
!! GAGTGTGATTTTGAAGGCCA+GGG + chr2_1:6000501-6000520 Msa0190500:CDS 45.0%
!! AGTGTGATTTTGAAGGCCAG+GGG + chr2_1:6000502-6000521 Msa0190500:CDS 45.0%
! GGACTTTGAAAGCCGAGTAA+AGG + chr2_1:6000632-6000651 Msa0190500:CDS 45.0%
!! AGGACTTTGAAATCCGAGTG+AGG + chr2_1:6000715-6000734 Msa0190500:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr2_1:6000757-6000776 Msa0190500:CDS 45.0%
! AGGACTTTGAAAGCCGAGTT+AGG + chr2_1:6000757-6000776 Msa0190500:CDS 45.0%
GAGGAACTTGAGCTAAGAGA+CGG + chr2_1:6000882-6000901 Msa0190500:CDS 45.0%
CCTTACACTGTGGAATGATG+GGG - chr2_1:6001221-6001240 None:intergenic 45.0%
CCCTTACACTGTGGAATGAT+GGG - chr2_1:6001222-6001241 None:intergenic 45.0%
CCCATCATTCCACAGTGTAA+GGG + chr2_1:6001219-6001238 Msa0190500:CDS 45.0%
TCCCTTACACTGTGGAATGA+TGG - chr2_1:6001223-6001242 None:intergenic 45.0%
GTTGTTCGAGCAGATCAATG+TGG - chr2_1:6001272-6001291 None:intergenic 45.0%
CGCACATGAGGTTTAACATG+TGG - chr2_1:6001309-6001328 None:intergenic 45.0%
ATTGCTTCTTCTCGCACATG+AGG - chr2_1:6001321-6001340 None:intergenic 45.0%
TGCAAATCCAGTACCAGTTC+TGG + chr2_1:6001383-6001402 Msa0190500:CDS 45.0%
GCAAATCCAGTACCAGTTCT+GGG + chr2_1:6001384-6001403 Msa0190500:CDS 45.0%
CAAATCCAGTACCAGTTCTG+GGG + chr2_1:6001385-6001404 Msa0190500:CDS 45.0%
AAAAACCCCAGAACTGGTAC+TGG - chr2_1:6001393-6001412 None:intergenic 45.0%
! TGTAGAGCTGTTTGGGACTA+TGG + chr2_1:6001503-6001522 Msa0190500:CDS 45.0%
! GTAGAGCTGTTTGGGACTAT+GGG + chr2_1:6001504-6001523 Msa0190500:CDS 45.0%
! GAGCATTCGAGTTCGAATCT+TGG + chr2_1:6001923-6001942 Msa0190500:intron 45.0%
! AACCCTTTTCCCTTAGGAAC+CGG + chr2_1:6002003-6002022 Msa0190500:intron 45.0%
GAACTAAATCTGCAAGGTGC+AGG - chr2_1:6002156-6002175 None:intergenic 45.0%
AACATGCAGGTGCATGAAGA+TGG - chr2_1:6002379-6002398 None:intergenic 45.0%
!! AAGGCGAGTAGCTTTGGTAT+TGG + chr2_1:6002555-6002574 Msa0190500:exon 45.0%
! CTTTGGTATTGGAAGTAGGG+CGG + chr2_1:6002566-6002585 Msa0190500:three_prime_UTR 45.0%
TGGGATTCAGTTCTTGGATG+TGG + chr2_1:6002634-6002653 Msa0190500:three_prime_UTR 45.0%
GGGATTCAGTTCTTGGATGT+GGG + chr2_1:6002635-6002654 Msa0190500:three_prime_UTR 45.0%
!! GATAAAAAAAAAAAAAAAAA+GGG - chr2_1:5998879-5998898 None:intergenic 5.0%
!! TGATAAAAAAAAAAAAAAAA+AGG - chr2_1:5998880-5998899 None:intergenic 5.0%
CTCTTTGCTGGCAGTCTAGT+AGG - chr2_1:5999517-5999536 None:intergenic 50.0%
CTACTAGACTGCCAGCAAAG+AGG + chr2_1:5999515-5999534 Msa0190500:CDS 50.0%
!! ACTCGGATTAAGGAGCGTTG+AGG + chr2_1:5999624-5999643 Msa0190500:CDS 50.0%
AGTGTGACCGACGACTAGAA+GGG + chr2_1:6000172-6000191 Msa0190500:CDS 50.0%
!! AAAGGTGTCGGAGTCAAAGC+AGG + chr2_1:6000230-6000249 Msa0190500:CDS 50.0%
GCAGGAGGAATTCGAAAGTC+AGG + chr2_1:6000248-6000267 Msa0190500:CDS 50.0%
!! GAAAGCCAGGAAAAGGTGCT+CGG + chr2_1:6000303-6000322 Msa0190500:CDS 50.0%
!! TGATTTTGAAGGCCAGGGGA+AGG + chr2_1:6000506-6000525 Msa0190500:CDS 50.0%
! TTGATTCGAAGTCCTTCCCC+TGG - chr2_1:6000521-6000540 None:intergenic 50.0%
AGAGGAATTTGAAGGCCGAG+TGG + chr2_1:6000587-6000606 Msa0190500:CDS 50.0%
CAACCAAGTCCTGCCATTGA+TGG + chr2_1:6001069-6001088 Msa0190500:CDS 50.0%
CTTCCATCAATGGCAGGACT+TGG - chr2_1:6001075-6001094 None:intergenic 50.0%
! ACTGTGGAATGATGGGGTTC+TGG - chr2_1:6001215-6001234 None:intergenic 50.0%
CCCCATCATTCCACAGTGTA+AGG + chr2_1:6001218-6001237 Msa0190500:CDS 50.0%
AGGAGCAAAAACCCCAGAAC+TGG - chr2_1:6001399-6001418 None:intergenic 50.0%
! GCTGTTTGGGACTATGGGTT+TGG + chr2_1:6001509-6001528 Msa0190500:CDS 50.0%
CTCCGGTTCCTAAGGGAAAA+GGG - chr2_1:6002008-6002027 None:intergenic 50.0%
ACTCCGGTTCCTAAGGGAAA+AGG - chr2_1:6002009-6002028 None:intergenic 50.0%
CGTAACACTCCGGTTCCTAA+GGG - chr2_1:6002015-6002034 None:intergenic 50.0%
TCGTAACACTCCGGTTCCTA+AGG - chr2_1:6002016-6002035 None:intergenic 50.0%
GAGAGAAAGGCGAGTAGCTT+TGG + chr2_1:6002549-6002568 Msa0190500:exon 50.0%
CACTCTCCTCAGCCACACAA+AGG + chr2_1:5998816-5998835 Msa0190500:five_prime_UTR 55.0%
!! CGGATTAAGGAGCGTTGAGG+AGG + chr2_1:5999627-5999646 Msa0190500:CDS 55.0%
AGCGCATGTCCGTTTCATCG+AGG + chr2_1:5999804-5999823 Msa0190500:CDS 55.0%
GCGCATGTCCGTTTCATCGA+GGG + chr2_1:5999805-5999824 Msa0190500:CDS 55.0%
CAGTGTGACCGACGACTAGA+AGG + chr2_1:6000171-6000190 Msa0190500:CDS 55.0%
GTGTGACCGACGACTAGAAG+GGG + chr2_1:6000173-6000192 Msa0190500:CDS 55.0%
TCAGGACAAGGCAAGAGACC+AGG + chr2_1:6002424-6002443 Msa0190500:intron 55.0%
CTGATCGAGACACAAGGGGA+AGG + chr2_1:6002739-6002758 Msa0190500:three_prime_UTR 55.0%
! GGTGTCGGAGTCAAAGCAGG+AGG + chr2_1:6000233-6000252 Msa0190500:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 5998713 6002945 5998713 ID=Msa0190500;Name=Msa0190500
chr2_1 mRNA 5998713 6002945 5998713 ID=Msa0190500-mRNA-1;Parent=Msa0190500;Name=Msa0190500-mRNA-1;_AED=0.11;_eAED=0.11;_QI=156|0.75|0.8|1|0.75|0.6|5|380|802
chr2_1 exon 5998713 5998837 5998713 ID=Msa0190500-mRNA-1:exon:2714;Parent=Msa0190500-mRNA-1
chr2_1 exon 5999330 6000951 5999330 ID=Msa0190500-mRNA-1:exon:2715;Parent=Msa0190500-mRNA-1
chr2_1 exon 6001037 6001546 6001037 ID=Msa0190500-mRNA-1:exon:2716;Parent=Msa0190500-mRNA-1
chr2_1 exon 6002167 6002336 6002167 ID=Msa0190500-mRNA-1:exon:2717;Parent=Msa0190500-mRNA-1
chr2_1 exon 6002428 6002945 6002428 ID=Msa0190500-mRNA-1:exon:2718;Parent=Msa0190500-mRNA-1
chr2_1 five_prime_UTR 5998713 5998837 5998713 ID=Msa0190500-mRNA-1:five_prime_utr;Parent=Msa0190500-mRNA-1
chr2_1 five_prime_UTR 5999330 5999360 5999330 ID=Msa0190500-mRNA-1:five_prime_utr;Parent=Msa0190500-mRNA-1
chr2_1 CDS 5999361 6000951 5999361 ID=Msa0190500-mRNA-1:cds;Parent=Msa0190500-mRNA-1
chr2_1 CDS 6001037 6001546 6001037 ID=Msa0190500-mRNA-1:cds;Parent=Msa0190500-mRNA-1
chr2_1 CDS 6002167 6002336 6002167 ID=Msa0190500-mRNA-1:cds;Parent=Msa0190500-mRNA-1
chr2_1 CDS 6002428 6002565 6002428 ID=Msa0190500-mRNA-1:cds;Parent=Msa0190500-mRNA-1
chr2_1 three_prime_UTR 6002566 6002945 6002566 ID=Msa0190500-mRNA-1:three_prime_utr;Parent=Msa0190500-mRNA-1
Gene Sequence

>Msa0190500

ATGGTTGTTAGGGCTTCTAAAAGAAAAAAGTTGCTGAATTTATATGACTCTAAGTCTGATGATGATTATGATGTACCTTTGAGAGCAAAGTCATTGTCAACTTTGAATTTGGGAGCAAGTAGTAGTAGTAGTAGTAAATCTATTGAGAATAGGCCTACTAGACTGCCAGCAAAGAGGCCTTTTGATGACAGCATTTCCTCGCAAACTTCGGTTAAAAAATCAAAGTTGTCATCGTTTTCCATTAAGCTCATGAAGAAGAGCATACTCGGATTAAGGAGCGTTGAGGAGGAAAAATTGCAATCCCTTGAGAGAGATATTGAGGAGTGCTCCAAAGAGCTTCTAAATAAGAAAAAGCAAGCTAGTGATGTTAGACGAACTAACCAATATTACGAGGAAATGCAGAACAAAATCGAAAAAGGTGTCAAAGATCTTGCAGCAAACGAAGCGCATGTCCGTTTCATCGAGGGTTTGATTAAAAACAAAACACTTGAGCTCAAGAAAAATAAGAGACAACTTCTTACAGCCATGGATAGCAATAGTAAAGGCCGTGGAAGATTGAAGGAAAAAGAGCTAGAGACTCTTTCACAAAAAATTGCTGAATGTACTCAGGAAATTAAGACGAGAAAGGAAGAGCTTGATGCTTCGAAAATATCTGTTAATCATAAAATAAAAGAACTAATGTCAGAAAGGAGTAACATACTAAATGTAATGTCAGAGAGGAGAACTGGTCAGCTTGTTCAAATGAAGGACTTGGAGTCAACGGAAAAGCAATTTGAAGGTGGAGCTACAGAGTTTGATTCCAAAATGAAGCAGTGTGACCGACGACTAGAAGGGGTTGAATCAAATGAGAAGCTCTATAAAGGGCAGACAAAGGTGTCGGAGTCAAAGCAGGAGGAATTCGAAAGTCAGGTGAAGGAGCTTGAATCAAAAAAGAAGCAATTTGAAAGCCAGGAAAAGGTGCTCGGATTAAAAGAGAAGCTATTTCAAAGACAAGTAGATGATCTGGAATCATGCAAGGAGCATTTTGAAAGCCAACTGAAGGGACTCAAATCAAAAGAGAAGATATTTGAAAGACGGATGAAGGAGTTGAAGTCAAAAGAGGAACGTTTTCAAAGACGAATGAAGGAGTTTGAATTAAGAGAGTGTGATTTTGAAGGCCAGGGGAAGGACTTCGAATCAAAATTGAAGCAATATGAAGGACAAGTGAAGGAGTTACAATCAAAGAGAGAGGAATTTGAAGGCCGAGTGGAAGAGTTTAAATCACAAGAGAAGGACTTTGAAAGCCGAGTAAAGGGGTTTGAATCAAAAAAGAAGGACTTTGAAAGCCGAGTGACGAAGTTTGAAACAGTAGAGAAGGACTTTGAAATCCGAGTGAGGAAGTTCAAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGTAGAGAAGGACTTTGAAAGCCGAGTTAGGAAGTTTGAATCAGCAGAGAAGGACTTTGAAAGCCGAGTGAAGAAGTTTGAATCAAAAGAAGAGGAACTTGAGCTAAGAGACGGTCAATATGAAACGTTAATAAAATCATTTGAAGAGGAAATTGAATCAGCTAATTATGCAATTTCATCTTTCACAGATGATCAACCAAGTCCTGCCATTGATGGAAGAAGTTTGCAGTTTCTCCCAATTGAGGAAATTGATGAACTTGAGTCACACGGCAATGACAGTCTAGCTAATCTGCTAGCATCATCATCAGACCCATCAAAAGATGTTTTAGATATAATCCAGAACCCCATCATTCCACAGTGTAAGGGAGAAAATGTTGTAATTATTGATGATCACCACATTGATCTGCTCGAACAACTGATGAGAATCTCACCACATGTTAAACCTCATGTGCGAGAAGAAGCAATGAAGCTAGCACTTAAACTGAAAGCTTACATTGGAGAAAATACTGCAAATCCAGTACCAGTTCTGGGGTTTTTGCTCCTTCTGTCAATTTATGGATTGGTTTCTTCTTTCGATGAAGATGAAGTTTTAAAGCTTTTTGGGTTTGCTGCTCAACACAAGATATCTGTAGAGCTGTTTGGGACTATGGGTTTGGCACATAAAGTTTCTGATTTAGTTCAGAATCTTATCATGAAGCAGCAATATATTGAAGCTGTTAGATTCATTTGTTATAACACGGCCACCAAAAATCAATCAGTTGATCTATTGCGGGAACATGTGCAGAATGCAAGAACATTTTATGAGAGCAGTTGCAAGGCAACCAACTCCATTGAAATCAAGGACAAGGCAAGAGACCAGGAAATTGCTAGTCTGAGAACTGTTCTACAGTGTCTTTCAGATAACAATATTGAATCTGCGGATTTACTCAACGAGATTCACAGTAGAATTCTTGAGCTAAATAGAGAGAAAGGCGAGTAG
Protein sequence

>Msa0190500

MVVRASKRKKLLNLYDSKSDDDYDVPLRAKSLSTLNLGASSSSSSKSIENRPTRLPAKRPFDDSISSQTSVKKSKLSSFSIKLMKKSILGLRSVEEEKLQSLERDIEECSKELLNKKKQASDVRRTNQYYEEMQNKIEKGVKDLAANEAHVRFIEGLIKNKTLELKKNKRQLLTAMDSNSKGRGRLKEKELETLSQKIAECTQEIKTRKEELDASKISVNHKIKELMSERSNILNVMSERRTGQLVQMKDLESTEKQFEGGATEFDSKMKQCDRRLEGVESNEKLYKGQTKVSESKQEEFESQVKELESKKKQFESQEKVLGLKEKLFQRQVDDLESCKEHFESQLKGLKSKEKIFERRMKELKSKEERFQRRMKEFELRECDFEGQGKDFESKLKQYEGQVKELQSKREEFEGRVEEFKSQEKDFESRVKGFESKKKDFESRVTKFETVEKDFEIRVRKFKSVEKDFESRVRKFESVEKDFESRVRKFESAEKDFESRVKKFESKEEELELRDGQYETLIKSFEEEIESANYAISSFTDDQPSPAIDGRSLQFLPIEEIDELESHGNDSLANLLASSSDPSKDVLDIIQNPIIPQCKGENVVIIDDHHIDLLEQLMRISPHVKPHVREEAMKLALKLKAYIGENTANPVPVLGFLLLLSIYGLVSSFDEDEVLKLFGFAAQHKISVELFGTMGLAHKVSDLVQNLIMKQQYIEAVRFICYNTATKNQSVDLLREHVQNARTFYESSCKATNSIEIKDKARDQEIASLRTVLQCLSDNNIESADLLNEIHSRILELNREKGE*